MD08G1120000.v1.1

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013097
Physical Location & Seq
Reverse (-)
11023459 .. 11037356
13898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1120000.v1.1.491

Sequence Viewer

Length: 6456 bp
ATGGTAGAGGGTTCGCCCGATGCCAAAGGCACTTTGGAGAATTATTTGTTGGGTTCACAGGACAATAGTATCTCCCCAAAGCCTTCGCACAGAGTTTGTGATTCATTAGCCCAGCAAGATCAAGTTCAAAGGAATTTGACATCAGTGATGGATAGTTCCTTGAAAGATGAATTTAAACAGCTCCCCTTGTCTTATCAACTGCACTCTCAAGCTAATAATGCCTCCAAAGAAAATCAGAAGGAAACATCCGAGGGATTAACCAAGGTGGGTGATGGTGCAGTTGGAGATACGGTGAAGGATTACTTAGCTTTCACAGAAGGTGGGGAAAGTGCAGAACTTAAAGAGTTTGCAGCAGACTTTTTGTCTTTGTATTGCAGTGATGTTCAACCAAATGAAAGTACTCTGCCAGAGATGAAAGTAAATGACCATAAGAGACAAGCTAGCCCATCTCAGCTTGACCGGGAGGACAAGATATTTAAAAAGAGGCATCTCATTACCAATCAGTCACACATGGAACATGAATCAAGTTGCTGCAGTGAGAAGAGTTCTGAAGCCATGCAGTCTGGTCGTCCTGATGAAAATAGGGTTACCATCGTTAATGAACTGCTCGAGCTCCAGTCAACCTTGAAAACATGCAGTAATACACCAAAACAATTTTTAGACATGTTTAAATGCTATACACCAGGTTCGATGACTAGAAAATCTATTGTTAGTGAAACCCCCAAGTCACGTGGAAGCTCATCATTTTCACCTGGAGAAGCTTTTTGGAATGATGCCATTCAACTTGCTGATGGCCTGTGTGCACAAGCAGCAGAAGTAATTAGTGCTGCAGACATCCAATATCATAGTAAAAGTTCATGCAATTTGAGAAATGCACCCTGTGATGGCAAGTCAAAGGAAATGCTGAATGAAGGTGAGCGCATGGATAACAGTGCTAATTCAGGGCCAAAGGGGAAGTATAGAAAGGATTTAGATAAAGAAGTGTCCCCACTGCCGGTTAAACATTTTGATTTTTCTTGCGAAGACAAAAATTTGGGTGAAGTTGTACCACATCATTGTGATGCATACAATTTAAAAAGCGCAGCTCTTATAGGTGGTGAGCAATCTGAATCCAGCCTTGCAGACCATAGAGGTCTGAGAAATACTATCATGACCCGTTGCAGCAAATCTCAGGAAAATCAAGTCATATTTCGAGAACCAGATACAGATACTGTTAATGCAGTGACCAACATGAAAGTGGATTTGACAGGCAAAGATAATGATAGCATGACATCTTATTCACCAGTTGATGAAGTTGTGAAGTTGACTGGTAATCATGAATCTAGCGAAGCAAGTACTCCTTCAAGCTCTATGCCTCTCAAAGATCGCCTCGATCTGAACAGTTGGCTTCCACCAGAAATATGCAGCATATACAGAAAGAAAGGAATTTCAAAACTGTATCCTTGGCAGGTTGATTGCCTTCAGGTGGATGGTGTATTGCAGAGAAGGAATCTCGTATATTGTGCATCAACAAGTGCTGGCAAAAGTTTTGTTGCAGAAATTCTAATGTTGCGGCGGGTCTTATCAACTGGAGCAATGGCTTTACTTGTACTGCCTTATGTATCAATTTGTGCAGAAAAGGCAGAACATTTGGACGTACTTCTTGAACCACTTGGTAAGCATGTTCGTAGTTATTACGGAAACCAAGGTGGTGGAACACTTCCCAAAGATACTTCTGTAGCTGTTTGCACAATAGAAAAGGCAAACTTTTTGATAAACAGGCTGCTGGAAGAGGGTCGTCTTTCAGAAATTGGGATTATTGTGATTGATGAATTGCACATGGTTGGAGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACCAAACTTCGTTATGCAGCTGGTGAAGGCAATTCTGGATCAAGTAGTGGAGAAAGTTCAGGTATGAGTAGTGGTAAGGCTGACACTGCTCATGGTCTGCAAATTGTTGGGATGAGTGCAACTATGCCAAATGTGGCAGCCGTTGCTGATTGGCTTCAGGCTGCACTATACCAGACAGAATTTCGACCAGTACCATTAGAAGAATATATTAAAGTTGGTAACACCCTTTATAACAAGAAAATGGATATTGTTAGAACATTTCCTAAAGCAGCTGACCTTGGTGGTAAAGATCCTGATCATGTTGTGGAGCTGTGCAATGAGGTTGTTCAAGAGGGTCTTTCCGTGCTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCAAGGCATGTTTCAAGATTCCTCAAGAAATTTTCACCTAACATTCGCAGTGATGACAGTGAATTTCATGATGTTGCTTTGGCTATTGATGCCTTACGAAGGTGCCCTGCTGGTTTGGATCCAGTATTAGAAGAAACTCTTCCTGCTGGTGTTGCCTATCACCATGCTGGCCTCACGGTTGAGGAGAGAGAGATTGTCGAAAATTGCTACCGTAGAGGCCTGGTACGTGTCTTAACTGCTACATCTACCTTAGCAGCTGGGGTTAATCTACCAGCTAGGCGGGTCATTTTTCGTCAGCCCAAGATTGGTCGTGATTTTATTGATGGGACAAGGTACAGACAGATGGCTGGTCGGGCTGGTCGAACTGGAATAGATACAAAAGGAGAAAGTGTACTTATTTGCAAGCCAGAAGAGATCAAAAGAATTATGGGAGTTCTTACTGAAAGCTGTCTGCCATTGCGTTCTTGTCTATCTGAAGATATGAATGGAATGACCCATGCTATCTTAGAAGTTGTAGCTGGGGGAATGGTTCAAACTGCTAATGACATTCATCGATATGTTAGGTGCACACTTCTAAACTCCACTAAACCATTTCAAGATGTGGTGAAATCAGCACAGGAATCTTTGAGGTGGTTGTGCCACAGGAAATTTCTAGAATGGAATGAGGATACTAAGTTATACAGTATCACACCTCTTGGACGTGCAGCATTTGGCAGTTCCCTCTGCCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCAAGAGAAGGGTTTGTGCTTGCATCTGATTTGCATTTAGTTTACCTAGTAACACCAATAAATGTTGATGTTCAGCCTGATTGGGAACTGTATTATGAACGGTTCATGGAATTGTCTGCTCTTGACCAGTCTGTTGGCAGTCGTGTTGGAGTGACAGAACCATTTTTGATGCGCATGGCACATGGTGCACCTGTGCGTTCATCAAACAGGTTCAGAGAAAATCTGAAACCGCTGCATGGAAAATATGAAAGTGGACCTGGAATGAACAACGATACTGTACTTCAGGATGATCAAATACTTCGAGTGTGTAAACGGTTCTATGTTGCTCTCATCCTGTCAAGACTAGTTCAGGAAGCTGCTATCATTGAGGTTTGTGAAGCTTTCAAAGTGGCCAGAGGTATGGTGCAGGCCTTACAAGAGAATGCTGGAAGGTTTGCATCTATGGTAACCATGTTTTGTGAGAGGCTTGGATGGCATGATTTCGAGGGCTTGGTTTGCAAGTTCCAAAATCGTGTTTCATTTGGAGTGAGAGCCGAGATTGTAGAGCTTACTACAATTCCATATGTCAAGGGTTCTCGAGCAAGGGCACTCTATAAAGCTGGCTTGCGTACTTCTTTAGCAATTGCTGAAGCGTCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCATCATGGAATGAACAAGAAGGTTCAGCTCAAAGGCGCATCCAAGTAGGCGTAGCAAAGAAGATCAAGAATGGTGCACACAAAATAGTTCTTGAAAAAGCTGAAGAGGCAAGAGTTGCTGCCTTCTCAGCTTTCAAAGCTCTTGGATTAGATGTCCCACAATTTTCTCGACCGGTATTTTTAACTGGCGGTGGAAGTCCCAGTATGCAAGGAGCAGGAAATTCCTCTGGTGATAACTCCACTAGTAGCTTTCCTAATGTAGAGCACAGCACAGAGTATGCTGCTAAGCCATCTTTGGAAGGTAGGGTCCATTCTGAAAAGGTTGCAGAAGTAAGGTCAACTGGTGTCATGCAAATTAAGTCTGGTCCTGAAAATTCTAAAGTACATATTCGAGGGTCTGCTACCCCCGTAGATGAGCTTACTGTTGTGGTTGATCAAAACAAAAATACTGATTTGCCTGACCGGGTTCAGTTACAAAGCTTAGTTGATAGAACTAGGGCATCTGATGAGTCTCACGATCTTGAAAAACAGGAACGATGCAGAAGGGATAATTTGTCCTCTGGCTATAAGGGCAATGCTTGTGAGAAAGGCCCAATTCATGCAGTTAATACTCTTGGTGGTTTTGATTCCTTCTTGGATCTCTGGCAATCCACCTCAGAGTTCTATTTTGATATTCACTACAACAAAAGATCAGAATTCAATTCTGTTGCGCCCTTTGAAATACATGGCATAGCCATCTGTTGGGAAAACTCTCCTGTGTACTATGTCAATATTCCTAGGGATCTACTGTGCTCAGACAGCGGTAAAAATGAATGCTTGAATTTGAATGGATCCGGCAATAGAAGTAATGGCTTACCTCTTGATGATTTGTTGGAGATGGCTGAATGTAGATGGAACAGGATTGGTAAAATAATGGGAAAAAGAGGTGTTAGAAAAATTACTTGGAAGTTGAAAGTTCAGATTCAGGCACTTAAAAGTCCTGCAGTTCATACTCAGAGATTTGGTTGTCAGAATCTCGGAGGGAAAAGTGCTTGTTTTGAAATCATAGACAGCTCATTTTTATTGTTGTCTCCAGTTCATATAAAAGATGGAATTGACATGTGCATCGTGGCCTGGGTTCTTTGGCCTGATGAGGAGAGAAGCTCTAATCCTAACCTGGAAAAGGAAGTTAAGAAAAGGTTATCCAGTGAAGCTGCGGCAGCTGCTAATCGGAATGGAAGGTGGAAGAATCAGATGCGAAGAGCTGCACACAACGGTTGCTGCCGTCGGGCTGCACAAATACGGGCCTTATGTTCTGTTCTTTGGAAGTTACTTGTTGCTGAAGGCCTTACTGAAGCCCTCCTAAATATTGAAATCCCATTGGTTAATATTCTTGCTGACATGGAGCTATGGGGAGTGGGTCTTAACATGGAGGGATGCTTACAAGCACGCAAGGTGTTAGGAAAAAAGCTAGGACAGCTTGAGAAGGAAGCTTACAAGCTAGCTGGCATGAAATTTTCGTTATCTACGGCAGCAGAAATTGCTAATGTATTGTATGGACACTTAAAGCTGCCCATACCAGAGGGGCGCAATAAGGGGAAGCAACATCCAAGTACGGACAAGCATTGCTTGGATTTATTAAGGGATGAGCATCCTATAATTCCCGTCATTAAAGAGCATCGTACATTGGCAAAACTCTTAAACTGTACATTGGGATCAATTTGTTCACTGGGTAGGCTGTCCGTGAGGACACAAAAGTACACATTACATGGTCATTGGCTCCAAACATCAACAGCAACTGGTCGGCTTTCAATGGAGGAACCAAATCTCCAGTGTGTTGAACATATGGTTGACTTTAAAATGAATGAGGATGGAAATGGAAACAAAACTGATGTTGATCAATTCAACATCAATGCTCGTGACTACTTCATTCCTACTCAGGACAACTGGTTACTGTTAACTGCAGATTATTCTCAGATTGAATTGCGTCTGATGGCACATTTCTCTAAAGACTCTGCACTCACTGAACTCCTTAGTAATCCCCATGGTGATGTCTTCACCATGATAGCAGCTAGATGGACTGGGATTTCAGAAGAGTCTGTTAGTTCCCATGTACGAGATCAGACCAAAAGGTTGGTGTATGGCATTCTTTATGGAATGGGTGCTAATAGCCTTGCAGAACAACTGGACTGCAGTCCAGAAGAAGCTTCCGAAAAAATAAAGAACTTTAGAAGTTCTTTTCCTGGAGTTGCTTCTTGGCTTAATGAAGCAGTTGCATATTGCCGCACGAAAGGATACATCGAAACCCTTAAGGGAAGAAAGCGCTTTTTGTCAAAAATAAAACTAGGAAACAGTGAAGAAAAGTCGAAGGCTCAGAGACAAGCTGTGAATTCTATATGTCAGGGATCTGCTGCTGACATAATTAAGATTGCAATGATAAATATATATTCCGTGATAGTTGAAGGTGTTGAAAGGCCTGATTCTTCTTCTCATCCTGCAACAAACTTACACATACTGAAGGGCCGCTGCCGAATTTTATTACAGGTACATGATGAATTAGTACTAGAAGTTGATCCTGTTGTGATAAAGGAAGCTGCTTTGCTGCTTCAGACGAGCATGGAAAATGCTGTTTCGCTTCGTGTTCCATTGGATGTCAAAGTGAAAGTCGGAAGATCGTGGGGTTCCTTGCAACCTTTTCAGGCCGACCAATATAAAGATAAAGCTGTTGGAAATTCACTATATATTCTGGCTAAGCAATGCTTTCCTTGGAAAGATGCTGGACTGGACTGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2152

Amino Acids

238.15

Weight (kDa)

6.65

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 492 - 667 3.7e-17 DEAD/DEAH box helicase
Helicase_C PF00271 796 - 872 2.3e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 877 - 968 5.3e-24 Helix-turn-helix domain
POLQ_helical PF21099 992 - 1176 2e-51 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1182 - 1256 4.8e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1702 - 2114 2.3e-111 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2094
Acc16I TGCGCA 1 cut(s) 3185
Acc36I ACCTGC 1 cut(s) 1438
AccB1I GGYRCC 1 cut(s) 2349
AccB7I CCANNNNNTGG 1 cut(s) 4422
AciI CCGC 9 cut(s) 1552, 1555, 2527, 3242, 3870, 4482, 4877, 5944, 6184
AcoI YGGCCR 1 cut(s) 3402
AcvI CACGTG 1 cut(s) 731
AdeI CACNNNGTG 2 cut(s) 881, 3197
AfeI AGCGCT 1 cut(s) 5984
AfiI CCNNNNNNNGG 9 cut(s) 884, 994, 1465, 1838, 2346, 2552, 3248, 4422, 4843
AflII CTTAAG 1 cut(s) 5969
AflIII ACRYGT 3 cut(s) 663, 2473, 4779
AgeI ACCGGT 1 cut(s) 3853
AhdI GACNNNNNGTC 1 cut(s) 361
AhlI ACTAGT 2 cut(s) 3355, 3923
AjiI CACGTC 1 cut(s) 2948
AjnI CCWGG 7 cut(s) 682, 751, 2466, 3268, 4793, 4836, 5902
AjuI GAANNNNNNNTTGG 4 cut(s) 32, 64, 4606, 4638
AloI GAACNNNNNNTCC 2 cut(s) 5472, 5504
Alw21I GWGCWC 7 cut(s) 615, 805, 2816, 3202, 3760, 3948, 4475
Alw26I GTCTC 4 cut(s) 427, 4197, 4755, 6031
Alw44I GTGCAC 4 cut(s) 801, 2812, 3198, 3756
AlwNI CAGNNNCTG 2 cut(s) 1211, 5459
Ama87I CYCGRG 2 cut(s) 608, 3588
Aor51HI AGCGCT 1 cut(s) 5984
ApaLI GTGCAC 4 cut(s) 801, 2812, 3198, 3756
ArsI GACNNNNNNTTYG 2 cut(s) 6309, 6341
AsiGI ACCGGT 1 cut(s) 3853
Asp700I GAANNNNTTC 1 cut(s) 2385
AspA2I CCTAGG 1 cut(s) 4457
AspLEI GCGC 7 cut(s) 921, 1082, 3186, 3720, 4393, 5250, 5985
AspS9I GGNCC 7 cut(s) 944, 3266, 3988, 4046, 4271, 4965, 6181
AsuC2I CCSGG 2 cut(s) 461, 4145
AsuNHI GCTAGC 2 cut(s) 440, 5161
AvaI CYCGRG 2 cut(s) 608, 3588
AvaII GGWCC 3 cut(s) 3266, 3988, 4046
AvrII CCTAGG 1 cut(s) 4457
BaeGI GKGCMC 6 cut(s) 805, 2354, 2816, 3202, 3601, 3760
BaeI ACNNNNGTAYC 4 cut(s) 52, 85, 1701, 1734
BalI TGGCCA 1 cut(s) 3404
BamHI GGATCC 2 cut(s) 2365, 4511
BanI GGYRCC 1 cut(s) 2349
BanII GRGCYC 1 cut(s) 615
BarI GAAGNNNNNNTAC 2 cut(s) 5138, 5170
BauI CACGAG 1 cut(s) 5577
BbrPI CACGTG 1 cut(s) 731
BbsI GAAGAC 2 cut(s) 1029, 5707
Bbv12I GWGCWC 7 cut(s) 615, 805, 2816, 3202, 3760, 3948, 4475
BceAI ACGGC 3 cut(s) 1988, 4929, 5205
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 7 cut(s) 684, 753, 2468, 3270, 4795, 4838, 5904
BciVI GTATCC 3 cut(s) 1449, 2908, 5948
BclI TGATCA 4 cut(s) 2158, 3301, 4114, 5557
BcnI CCSGG 2 cut(s) 461, 4145
BcoDI GTCTC 4 cut(s) 427, 4197, 4755, 6031
BcuI ACTAGT 2 cut(s) 3355, 3923
BfmI CTRYAG 6 cut(s) 532, 828, 1716, 4662, 5622, 5851
BfoI RGCGCY 1 cut(s) 5986
BfrI CTTAAG 1 cut(s) 5969
BfuAI ACCTGC 1 cut(s) 1438
BfuI GTATCC 3 cut(s) 1449, 2908, 5948
BlnI CCTAGG 1 cut(s) 4457
BlpI GCTNAGC 2 cut(s) 3966, 6411
BmcAI AGTACT 3 cut(s) 400, 1336, 6222
Bme1390I CCNGG 9 cut(s) 461, 684, 753, 2468, 3270, 4145, 4795, 4838, 5904
Bme18I GGWCC 3 cut(s) 3266, 3988, 4046
BmeRI GACNNNNNGTC 1 cut(s) 361
BmeT110I CYCGRG 2 cut(s) 608, 3588
BmgBI CACGTC 1 cut(s) 2948
BmgT120I GGNCC 7 cut(s) 944, 3266, 3988, 4046, 4271, 4965, 6181
BmiI GGNNCC 7 cut(s) 2351, 2367, 3989, 4513, 5441, 5481, 6343
BmrFI CCNGG 9 cut(s) 461, 684, 753, 2468, 3270, 4145, 4795, 4838, 5904
BmrI ACTGGG 3 cut(s) 3876, 5399, 5751
BmtI GCTAGC 2 cut(s) 444, 5165
BmuI ACTGGG 3 cut(s) 3876, 5399, 5751
BoxI GACNNNNGTC 1 cut(s) 5853
BpiI GAAGAC 2 cut(s) 1029, 5707
BplI GAGNNNNNCTC 2 cut(s) 4808, 4840
BpmI CTGGAG 6 cut(s) 599, 774, 1590, 4737, 5474, 5925
Bpu10I CCTNAGC 1 cut(s) 2497
Bpu1102I GCTNAGC 2 cut(s) 3966, 6411
BpuEI CTTGAG 3 cut(s) 192, 2255, 5162
BpuMI CCSGG 2 cut(s) 461, 4145
Bsa29I ATCGAT 1 cut(s) 2800
BsaAI YACGTR 2 cut(s) 731, 2474
BsaBI GATNNNNATC 4 cut(s) 2157, 2239, 5310, 5556
BsaJI CCNNGG 9 cut(s) 249, 261, 1442, 1684, 2140, 4457, 4794, 5704, 6425
BsaWI WCCGGW 1 cut(s) 3853
BsaXI ACNNNNNCTCC 6 cut(s) 276, 306, 2622, 2652, 5472, 5502
Bsc4I CCNNNNNNNGG 9 cut(s) 884, 994, 1465, 1838, 2346, 2552, 3248, 4422, 4843
Bse118I RCCGGY 2 cut(s) 994, 3853
Bse3DI GCAATG 7 cut(s) 1581, 2185, 2702, 4261, 5284, 6099, 6422
Bse8I GATNNNNATC 4 cut(s) 2157, 2239, 5310, 5556
BseBI CCWGG 7 cut(s) 684, 753, 2468, 3270, 4795, 4838, 5904
BseCI ATCGAT 1 cut(s) 2800
BseDI CCNNGG 9 cut(s) 249, 261, 1442, 1684, 2140, 4457, 4794, 5704, 6425
BseJI GATNNNNATC 4 cut(s) 2157, 2239, 5310, 5556
BseLI CCNNNNNNNGG 9 cut(s) 884, 994, 1465, 1838, 2346, 2552, 3248, 4422, 4843
BseMI GCAATG 7 cut(s) 1581, 2185, 2702, 4261, 5284, 6099, 6422
BseRI GAGGAG 2 cut(s) 2444, 4829
BseSI GKGCMC 6 cut(s) 805, 2354, 2816, 3202, 3601, 3760
BseYI CCCAGC 3 cut(s) 111, 2504, 2765
Bsh1285I CGRYCG 1 cut(s) 3854
BshNI GGYRCC 1 cut(s) 2349
BshTI ACCGGT 1 cut(s) 3853
BshVI ATCGAT 1 cut(s) 2800
BsiEI CGRYCG 1 cut(s) 3854
BsiHKAI GWGCWC 7 cut(s) 615, 805, 2816, 3202, 3760, 3948, 4475
BsiHKCI CYCGRG 2 cut(s) 608, 3588
BsiSI CCGG 5 cut(s) 460, 995, 3854, 4144, 4515
BslFI GGGAC 4 cut(s) 970, 2587, 3821, 3864
BslI CCNNNNNNNGG 9 cut(s) 884, 994, 1465, 1838, 2346, 2552, 3248, 4422, 4843
BsmAI GTCTC 4 cut(s) 427, 4197, 4755, 6031
BsmFI GGGAC 4 cut(s) 970, 2587, 3821, 3864
BsmI GAATGC 3 cut(s) 3439, 4499, 5805
BsoBI CYCGRG 2 cut(s) 608, 3588
Bsp1286I GDGCHC 9 cut(s) 615, 805, 2354, 2816, 3202, 3601, 3760, 3948, 4475
Bsp1407I TGTACA 1 cut(s) 5366
Bsp1720I GCTNAGC 2 cut(s) 3966, 6411
Bsp19I CCATGG 1 cut(s) 5704
BspACI CCGC 9 cut(s) 1552, 1555, 2527, 3242, 3870, 4482, 4877, 5944, 6184
BspDI ATCGAT 1 cut(s) 2800
BspHI TCATGA 3 cut(s) 1149, 1315, 2314
BspLI GGNNCC 7 cut(s) 2351, 2367, 3989, 4513, 5441, 5481, 6343
BspMAI CTGCAG 5 cut(s) 536, 832, 4666, 5626, 5855
BspMI ACCTGC 1 cut(s) 1438
BspOI GCTAGC 2 cut(s) 444, 5165
BspQI GCTCTTC 1 cut(s) 4915
BspT107I GGYRCC 1 cut(s) 2349
BspTI CTTAAG 1 cut(s) 5969
BsrDI GCAATG 7 cut(s) 1581, 2185, 2702, 4261, 5284, 6099, 6422
BsrFI RCCGGY 2 cut(s) 994, 3853
BsrGI TGTACA 1 cut(s) 5366
BssAI RCCGGY 2 cut(s) 994, 3853
BssECI CCNNGG 9 cut(s) 249, 261, 1442, 1684, 2140, 4457, 4794, 5704, 6425
BssSI CACGAG 1 cut(s) 5577
BssT1I CCWWGG 7 cut(s) 261, 1442, 1684, 2140, 4457, 5704, 6425
Bst2BI CACGAG 1 cut(s) 5577
Bst2UI CCWGG 7 cut(s) 684, 753, 2468, 3270, 4795, 4838, 5904
Bst6I CTCTTC 7 cut(s) 536, 1764, 2391, 2652, 3780, 4915, 5748
BstAFI CTTAAG 1 cut(s) 5969
BstAPI GCANNNNNTGC 4 cut(s) 2006, 3197, 3797, 5201
BstAUI TGTACA 1 cut(s) 5366
BstBAI YACGTR 2 cut(s) 731, 2474
BstDSI CCRYGG 1 cut(s) 5704
BstEII GGTNACC 2 cut(s) 586, 3457
BstENI CCTNNNNNAGG 2 cut(s) 2344, 4841
BstH2I RGCGCY 1 cut(s) 5986
BstHHI GCGC 7 cut(s) 921, 1082, 3186, 3720, 4393, 5250, 5985
BstMAI GTCTC 4 cut(s) 427, 4197, 4755, 6031
BstMCI CGRYCG 1 cut(s) 3854
BstNI CCWGG 7 cut(s) 684, 753, 2468, 3270, 4795, 4838, 5904
BstNSI RCATGY 6 cut(s) 636, 667, 1664, 2258, 4783, 6454
BstPAI GACNNNNGTC 1 cut(s) 5853
BstPI GGTNACC 2 cut(s) 586, 3457
BstSCI CCNGG 9 cut(s) 459, 682, 751, 2466, 3268, 4143, 4793, 4836, 5902
BstSFI CTRYAG 6 cut(s) 532, 828, 1716, 4662, 5622, 5851
BstSLI GKGCMC 6 cut(s) 805, 2354, 2816, 3202, 3601, 3760
BstV2I GAAGAC 2 cut(s) 1029, 5707
BstX2I RGATCY 7 cut(s) 1828, 2152, 2365, 4318, 4462, 4511, 6065
BstXI CCANNNNNNTGG 4 cut(s) 1691, 3146, 3412, 5794
BstYI RGATCY 7 cut(s) 1828, 2152, 2365, 4318, 4462, 4511, 6065
Bsu15I ATCGAT 1 cut(s) 2800
BsuI GTATCC 3 cut(s) 1449, 2908, 5948
BsuTUI ATCGAT 1 cut(s) 2800
BtgI CCRYGG 1 cut(s) 5704
BtrI CACGTC 1 cut(s) 2948
BtsI GCAGTG 6 cut(s) 382, 541, 989, 1227, 1947, 2302
BveI ACCTGC 1 cut(s) 1438
CaiI CAGNNNCTG 2 cut(s) 1211, 5459
CciI TCATGA 3 cut(s) 1149, 1315, 2314
CfoI GCGC 7 cut(s) 921, 1082, 3186, 3720, 4393, 5250, 5985
Cfr10I RCCGGY 2 cut(s) 994, 3853
Cfr13I GGNCC 7 cut(s) 944, 3266, 3988, 4046, 4271, 4965, 6181
ClaI ATCGAT 1 cut(s) 2800
CseI GACGC 2 cut(s) 3633, 5636
CsiI ACCWGGT 1 cut(s) 682
CspAI ACCGGT 1 cut(s) 3853
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
DraI TTTAAA 5 cut(s) 175, 478, 670, 1074, 5518
DraIII CACNNNGTG 2 cut(s) 881, 3197
DriI GACNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 1 cut(s) 3402
Eam1104I CTCTTC 7 cut(s) 536, 1764, 2391, 2652, 3780, 4915, 5748
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 7 cut(s) 536, 1764, 2391, 2652, 3780, 4915, 5748
Ecl136II GAGCTC 1 cut(s) 613
Eco130I CCWWGG 7 cut(s) 261, 1442, 1684, 2140, 4457, 5704, 6425
Eco147I AGGCCT 4 cut(s) 2466, 3422, 5007, 6136
Eco24I GRGCYC 1 cut(s) 615
Eco47I GGWCC 3 cut(s) 3266, 3988, 4046
Eco47III AGCGCT 1 cut(s) 5984
Eco53kI GAGCTC 1 cut(s) 613
Eco72I CACGTG 1 cut(s) 731
Eco88I CYCGRG 2 cut(s) 608, 3588
Eco91I GGTNACC 2 cut(s) 586, 3457
EcoICRI GAGCTC 1 cut(s) 613
EcoNI CCTNNNNNAGG 2 cut(s) 2344, 4841
EcoO65I GGTNACC 2 cut(s) 586, 3457
EcoRI GAATTC 2 cut(s) 4376, 6049
EcoRII CCWGG 7 cut(s) 682, 751, 2466, 3268, 4793, 4836, 5902
EcoT14I CCWWGG 7 cut(s) 261, 1442, 1684, 2140, 4457, 5704, 6425
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 1 cut(s) 615
ErhI CCWWGG 7 cut(s) 261, 1442, 1684, 2140, 4457, 5704, 6425
FaqI GGGAC 4 cut(s) 970, 2587, 3821, 3864
FauI CCCGC 2 cut(s) 1548, 2520
FauNDI CATATG 2 cut(s) 3574, 5505
FbaI TGATCA 4 cut(s) 2158, 3301, 4114, 5557
FriOI GRGCYC 1 cut(s) 615
FspAI RTGCGCAY 1 cut(s) 3185
FspI TGCGCA 1 cut(s) 3185
GlaI GCGC 7 cut(s) 920, 1081, 3185, 3719, 4392, 5249, 5984
GsaI CCCAGC 3 cut(s) 115, 2508, 2769
GsuI CTGGAG 6 cut(s) 599, 774, 1590, 4737, 5474, 5925
HaeII RGCGCY 1 cut(s) 5986
HapII CCGG 5 cut(s) 460, 995, 3854, 4144, 4515
HgaI GACGC 2 cut(s) 3633, 5636
HhaI GCGC 7 cut(s) 921, 1082, 3186, 3720, 4393, 5250, 5985
Hin6I GCGC 7 cut(s) 919, 1080, 3184, 3718, 4391, 5248, 5983
HinP1I GCGC 7 cut(s) 919, 1080, 3184, 3718, 4391, 5248, 5983
HincII GTYRAC 6 cut(s) 621, 1305, 1863, 4020, 5512, 5619
HindII GTYRAC 6 cut(s) 621, 1305, 1863, 4020, 5512, 5619
HindIII AAGCTT 5 cut(s) 759, 3390, 4159, 5151, 5865
HpaI GTTAAC 1 cut(s) 5619
HpaII CCGG 5 cut(s) 460, 995, 3854, 4144, 4515
Hpy99I CGWCG 1 cut(s) 4950
HpyCH4IV ACGT 4 cut(s) 730, 1635, 2473, 2947
HpySE526I ACGT 4 cut(s) 730, 1635, 2473, 2947
HspAI GCGC 7 cut(s) 919, 1080, 3184, 3718, 4391, 5248, 5983
Ksp22I TGATCA 4 cut(s) 2158, 3301, 4114, 5557
KspAI GTTAAC 1 cut(s) 5619
LguI GCTCTTC 1 cut(s) 4915
LmnI GCTCC 7 cut(s) 186, 618, 1571, 2170, 3893, 5065, 5445
MabI ACCWGGT 1 cut(s) 682
MaeII ACGT 4 cut(s) 730, 1635, 2473, 2947
MfeI CAATTG 1 cut(s) 3633
MflI RGATCY 7 cut(s) 1828, 2152, 2365, 4318, 4462, 4511, 6065
MhlI GDGCHC 9 cut(s) 615, 805, 2354, 2816, 3202, 3601, 3760, 3948, 4475
MlsI TGGCCA 1 cut(s) 3404
MluNI TGGCCA 1 cut(s) 3404
MlyI GAGTC 3 cut(s) 4199, 5666, 5765
MmeI TCCRAC 6 cut(s) 262, 1804, 3139, 4533, 6307, 6367
Mox20I TGGCCA 1 cut(s) 3404
Mph1103I ATGCAT 1 cut(s) 1066
MroXI GAANNNNTTC 1 cut(s) 2385
MscI TGGCCA 1 cut(s) 3404
MslI CAYNNNNRTG 5 cut(s) 1056, 1059, 1235, 2802, 5709
Msp20I TGGCCA 1 cut(s) 3404
MspA1I CMGCKG 7 cut(s) 1883, 2135, 2504, 3244, 4482, 4883, 6186
MspCI CTTAAG 1 cut(s) 5969
MspI CCGG 5 cut(s) 460, 995, 3854, 4144, 4515
MspR9I CCNGG 9 cut(s) 461, 684, 753, 2468, 3270, 4145, 4795, 4838, 5904
MunI CAATTG 1 cut(s) 3633
Mva1269I GAATGC 3 cut(s) 3439, 4499, 5805
MvaI CCWGG 7 cut(s) 684, 753, 2468, 3270, 4795, 4838, 5904
NciI CCSGG 2 cut(s) 461, 4145
NcoI CCATGG 1 cut(s) 5704
NdeI CATATG 2 cut(s) 3574, 5505
NheI GCTAGC 2 cut(s) 440, 5161
NlaIV GGNNCC 7 cut(s) 2351, 2367, 3989, 4513, 5441, 5481, 6343
NmeAIII GCCGAG 1 cut(s) 3571
NmuCI GTSAC 5 cut(s) 504, 726, 1222, 3163, 5579
NsbI TGCGCA 1 cut(s) 3185
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 6 cut(s) 636, 667, 1664, 2258, 4783, 6454
PaeR7I CTCGAG 2 cut(s) 608, 3588
PagI TCATGA 3 cut(s) 1149, 1315, 2314
PceI AGGCCT 4 cut(s) 2466, 3422, 5007, 6136
PciI ACATGT 2 cut(s) 663, 4779
PciSI GCTCTTC 1 cut(s) 4915
PcsI WCGNNNNNNNCGW 1 cut(s) 2605
PctI GAATGC 3 cut(s) 3439, 4499, 5805
PdmI GAANNNNTTC 1 cut(s) 2385
PflMI CCANNNNNTGG 1 cut(s) 4422
PfoI TCCNGGA 1 cut(s) 5902
PinAI ACCGGT 1 cut(s) 3853
PleI GAGTC 3 cut(s) 4198, 5666, 5764
PmaCI CACGTG 1 cut(s) 731
PmlI CACGTG 1 cut(s) 731
PpsI GAGTC 3 cut(s) 4198, 5666, 5764
Ppu21I YACGTR 2 cut(s) 731, 2474
PscI ACATGT 2 cut(s) 663, 4779
PshAI GACNNNNGTC 1 cut(s) 5853
PsiI TTATAA 1 cut(s) 2094
Psp124BI GAGCTC 1 cut(s) 615
Psp6I CCWGG 7 cut(s) 682, 751, 2466, 3268, 4793, 4836, 5902
PspCI CACGTG 1 cut(s) 731
PspEI GGTNACC 2 cut(s) 586, 3457
PspFI CCCAGC 3 cut(s) 111, 2504, 2765
PspGI CCWGG 7 cut(s) 682, 751, 2466, 3268, 4793, 4836, 5902
PspN4I GGNNCC 7 cut(s) 2351, 2367, 3989, 4513, 5441, 5481, 6343
PspPI GGNCC 7 cut(s) 944, 3266, 3988, 4046, 4271, 4965, 6181
PspXI VCTCGAGB 1 cut(s) 608
PstI CTGCAG 5 cut(s) 536, 832, 4666, 5626, 5855
PstNI CAGNNNCTG 2 cut(s) 1211, 5459
PsuI RGATCY 7 cut(s) 1828, 2152, 2365, 4318, 4462, 4511, 6065
PvuII CAGCTG 4 cut(s) 1883, 2135, 2504, 4883
RseI CAYNNNNRTG 5 cut(s) 1056, 1059, 1235, 2802, 5709
SacI GAGCTC 1 cut(s) 615
SapI GCTCTTC 1 cut(s) 4915
Sau96I GGNCC 7 cut(s) 944, 3266, 3988, 4046, 4271, 4965, 6181
ScaI AGTACT 3 cut(s) 400, 1336, 6222
SchI GAGTC 3 cut(s) 4199, 5666, 5765
ScrFI CCNGG 9 cut(s) 461, 684, 753, 2468, 3270, 4145, 4795, 4838, 5904
SduI GDGCHC 9 cut(s) 615, 805, 2354, 2816, 3202, 3601, 3760, 3948, 4475
SexAI ACCWGGT 1 cut(s) 682
SfcI CTRYAG 6 cut(s) 532, 828, 1716, 4662, 5622, 5851
Sfr274I CTCGAG 2 cut(s) 608, 3588
SinI GGWCC 3 cut(s) 3266, 3988, 4046
SlaI CTCGAG 2 cut(s) 608, 3588
SmiMI CAYNNNNRTG 5 cut(s) 1056, 1059, 1235, 2802, 5709
SmlI CTYRAG 6 cut(s) 207, 608, 2270, 3588, 5141, 5969
SmoI CTYRAG 6 cut(s) 207, 608, 2270, 3588, 5141, 5969
SpeI ACTAGT 2 cut(s) 3355, 3923
SseBI AGGCCT 4 cut(s) 2466, 3422, 5007, 6136
SsiI CCGC 9 cut(s) 1552, 1555, 2527, 3242, 3870, 4482, 4877, 5944, 6184
SspI AATATT 4 cut(s) 2214, 4453, 5029, 5050
SstI GAGCTC 1 cut(s) 615
StuI AGGCCT 4 cut(s) 2466, 3422, 5007, 6136
StyD4I CCNGG 9 cut(s) 459, 682, 751, 2466, 3268, 4143, 4793, 4836, 5902
StyI CCWWGG 7 cut(s) 261, 1442, 1684, 2140, 4457, 5704, 6425
TaiI ACGT 4 cut(s) 733, 1638, 2476, 2950
TauI GCSGC 4 cut(s) 1555, 4880, 5946, 6186
TseFI GTSAC 5 cut(s) 504, 726, 1222, 3163, 5579
Tsp45I GTSAC 5 cut(s) 504, 726, 1222, 3163, 5579
TspGWI ACGGA 5 cut(s) 1692, 2194, 5291, 5392, 6100
Van91I CCANNNNNTGG 1 cut(s) 4422
Vha464I CTTAAG 1 cut(s) 5969
VneI GTGCAC 4 cut(s) 801, 2812, 3198, 3756
VpaK11BI GGWCC 3 cut(s) 3266, 3988, 4046
XagI CCTNNNNNAGG 2 cut(s) 2344, 4841
XbaI TCTAGA 1 cut(s) 2899
XceI RCATGY 6 cut(s) 636, 667, 1664, 2258, 4783, 6454
XcmI CCANNNNNNNNNTGG 2 cut(s) 31, 1234
XhoI CTCGAG 2 cut(s) 608, 3588
XmaJI CCTAGG 1 cut(s) 4457
XmnI GAANNNNTTC 1 cut(s) 2385
ZrmI AGTACT 3 cut(s) 400, 1336, 6222
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.