FvH4_2g36240

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013097
Physical Location & Seq
Reverse (-)
26568829 .. 26584913
16085 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g36240.t2

Sequence Viewer

Length: 6537 bp
ATGGCGTCCGGTTCGCCTCCTCCGTCGCGCATCAATCAGTTCTTTGCTTCGAAGAAACGGAAGCCTGTGTCGCCTGCTTTGAAGTCTGGGAGAAATGAAAAGGATGCTAAAGCTGTAGAGACTTCACCTAGTGCCAAGGCCACATTGGACAGTTATCTGCGGACGTCGCCGGACAGTCATAATGTTGCGAAAGCTTCGTGCAAAATTGGTGATTCTTTGGCTCGGCAAGATCAAGTTAGAAGGAATTTGGCTTCGGAGATGGATAATTCTTCGAGAAATGAACCTATGCAGCTCCGTTTGTCTTCTCAATTACACACTCGTGCAAGTGAGGCTTCCGGAGCAAATCAGAAGGAAATATCCGAGGGAGTAGTTAAGGTGGATGATGTTGCAGCTGGAGGTTCTTCCAAGGATCAGTTGGATATTGCGGGAGGTGTGGAGAATGTAGAGATGAAGAAGTTTGCAGCCGACTTTTTGTCTTTGTATTGCAGTGATGTTCAATCAAACAAAAGTTCTATGTCAGAGCTGAAAGTGAATGATCATAAGAGACACGCTAGCGCATCTTTGCTTGACGGGGAGGATGAGATGCTTAAAAAGAGGCATTGCAGTAGTGATAAATCTTATGAAGAAAGTGAAACAAGCGGTTCTACAGAGAAGCGCTGTGAAGCAGTGCAATCTGTTTGTGTTGATAAAAATGGGGTTACCATTGCTAATGAATCACGCGAGTTTCAACAAACCTTGAAACCGTGCAGTAATACACATAAATTGTCTCTAGACACAGTTGATTGTTATACACCAGGCTCATTGATTAAATCATGTGTTCGTCAGACCCCCAATTCAACACGTGGTAGCTCATTGTCTTCACCTGGTGAAGCTTTTTGGAATGATGCGATTCAAGTTGCTGATGGTCTATGTGCTCAGGCACCTGAAGCACCTACTGCCTCAGATAGCCAGTATCATGGTGAGAACTCGTGCAGTTTGAGAAATGCACACTTTGATGGAAAGTCAAAGGAAATGATGGATGAAGGTGAGCGTATGGCTAACGATGCTGATTCAGAGCCGACGGCGAAGCATAGGAAGGACTTCTGTAAAGAAGTATCCCCACTGCCTGTTAAGCATTTTGACTTCTCTCGTGAGGACAAGATTTCGAATGAAAGTATGCCACATCGTATGAATTCGTTCAATTCGAAAAGCATGGCTCATGGAGGTGGCCAACAATCTGAATCTAGTCTTACAGATTTGAGAGGTCTAAGACATGGTAATACTATGGTCCGTTGCAGCAAATCTCAGGAAAATAAGGGGCAAGATGTTGATTCTGTTACTGCAGTGACCAAAATGAAGAATTTGTCTAGCGAGGACAATGGTGGCCCAACATTTTCTTCACCAGGTGATGATGTCATTAATTTGAATGGTAAAGGTGAATCTAGTGAAGCAAGTACTCCTTCAAGTTTTATGCCACTGAAAGATTGTCTGGATCTAGATGGTTGGCTTGCACCAGAAATATGCAGCATATATAGAAAGAAAGGAATTTCAAAACTTTATCCTTGGCAGGTTGAGTGCCTTCAGGTTGATGGTGTATTGCAGAGAAGGAATCTTGTGTATTGTGCATCAACGAGTGCAGGTAAAAGTTTTGTTGCAGAAATTCTGATGCTGCGACAGGTCTTGTCATCTGGAAAAATAGCTTTACTTGTACTTCCTTATGTATCACTTTGTGCAGAAAAGGCAGAACACCTGGAGCTACTTCTTGAACCACTTGGTAAGCGTGTTCGTAGTTATTATGGCAACCAAGGTGGTGGTACACTTCCCAAAGACACTTCTGTAGCTGTCTGCACAATAGAAAAGGCAAACTTCTTAATAAACAAGCTGCTGGAAGAGGGCCGTCTTTCAGAAATTGGAATTGTTGTGATAGATGAACTGCACATGGTTGGCGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACAAAACTTCGTTATGCAGCTGGTGAAGGCATTTCTGAATCAAGTAGTGGAGAAAGTTCAGGCATGAGCAGCGGTAAGGCTGATCCTGCTCATGGTTTGCAAATTGTTGGTATGAGTGCCACTATGCCAAATGTGGCAGCAGTTGCTGATTGGCTTCAAGCTGCCCTCTACCAGACAGAATTTCGACCGGTACCACTAGAAGAATACATTAAAGTGGGGAACACCATTTATAACAAGAAAATGGAGATTATTAGAACAATCCCCAAAGCAGTTGACCTTGGTGGTAAAGATCCAGATCATGTTGTGGAACTGTGCAATGAGGTTGTACAAGAGGGTTTTTCCGTGTTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCGAGGCATGTTTCAAGATTCCTTAAGAAATTTTCGGTCAACACTCGCACTGATGACAGTGAATTTAATGACTTGACATTGGCTATTGATTCCTTAAGACGGTGTCCTGCTGGTTTGGATCCAGTATTAGAAGAAACCCTTCCTTCTGGAGTTGCCTACCACCATGCTGGTCTCACGGTTGAGGAAAGAGAGATTGTTGAAACCTGCTACCGTAAGGGCCTTGTACGCGTCTTAACTGCCACATCTACCTTAGCAGCTGGTGTTAATCTGCCAGCTAGGAGGGTCATTTTCCGTCAGCCTCGGATTGGTCGCGATTTTATAGATGGGACACGATACAGGCAAATGGCTGGTCGGGCTGGTCGTACTGGCATAGATACAAAAGGAGAAAGTGTACTTATTTGCAAGCCAGACGAGATCAAAAGGATTATGGGAGTTCTTAATGAAAGCTGTCTGCCATTGCGCTCTTGTTTATCTGAAGATATGAATGGAATGACTCATGCAATCTTAGAAGTAGTAGCGGGGGGAATTGTTCAAACTGCTAAGGACATTCATCGCTATGTTAGGTGCACATTGTTAAACTCCACTAAACCTTTCCAGGATGTGGTGAAATCAGCACAGGAATCTCTCCGGTGGTTGTGCCACAGGAAATTTCTAGAATGGAATGATGACACTAAGCTATACAGTACCACACCCCTTGGACGTGCAGCTTTTGGCAGTTCTCTGTGTCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCTAGAGAAGGATTTGTACTTGCATCTGATTTGCATTTGGTTTACCTAGTAACACCAATAAATGTTGATGTTGAGCCTGATTGGGAACTGTATTATGAAAGGTTCATGGAATTGTCTTCTCTTGACCAGTCCGTTGGCAACCGCGTTGGAGTGACAGAGCCATTTTTGATGAGAATGGCACATGGTGCACCTATGAGATCATCAAGCAGGATGAGAGAAAATATGAAAGCGTTGAATGGCAATTATGAAAACCGATTTGGGACTACGAAGATTGTGCTTCCGGATGATCAAACAGTTCGAGTGTGTAAACGGTTATATGTTGCTCTCATCTTGTCAAGACTAGTTCAGGAAGTTGCTATCAATGAGGTATGTGAAGCTTTTAAAGTGGCTAGAGGTATGGTCCAGGCCTTACAAGAGAATGCCGGAAGGTTTGCATCTATGGTAACTATGTTCTGTGAGAGGCTTGGATGGCATGACCTGGAAGGCTTGGTTGGAAAGTTCCAAAACCGTGTCTCATTTGGAGTGAGAGCAGAGATTGTAGAACTGACTACAATTCCATTTGTCAAGGGTTCTCGAGCAAGGGCACTATATAAAGCTGGCTTGCGTACACCTTTAGCTATTGCTGAAGCATCCATCCCTGAAATAGTCAAAGCTCTTTTTGAATCTTCATCCTGGGCAGCCCAAGAAGGTTCAGCTCAAAGACGCATCCACGTAGGAGTAGCCAAGAAGATCAAGAAGGGTGCACACAAAATAGTTCTGGAAAAGGCTGAAGAGGCTAGAGTTGCTGCCTTCTCGGCTTTTAAATCACTTGGATTGGAGGTCCCACAGTTTTCTCAACCATTATTTCCAACTGCTGGTGGAAGTCCCAGTATGCAAGGAACAACAAATTCCTCTGGTGATGAAAATACTTGTAGCTTTGTTAATGCCCATGTGGATGATGCTCCTAAGCCATCTTTGGAAGTAAGGGTTCCTTCTGAAAAGGTTGTATCAGGAGAGAAGTTGACAAAAACATCAGATAGTGGTATGGTGGTGGCTGTAGGAGAAGTAAATTCAAGTGGTGCCATGCAAATTCAGTTTGGGGCTGAAAGTTCTGTAGTAGCAATTGAAGGGTCTGGTGCCCTTAGGGATGAGCTAAATGCTACTGTTCATCAAACCAAAATTGCTGACCCTAGCATTTCAATTATGGTAGACAGCTTAGGTGATAGAAATAGGATATCCAGTGTGTCTTTAGATCCTAAAAAACCGGGAAGCTGTGAAGGGGAAAATTTGTGTGTTGGCAAGGGCAAAGTTTGTGAAAAAGGCCCCATTCATGCAATTTATACTCCTGGTGGAATTGATTCATTTTTGAATATTTGGGAAACCACCTCAGAGTTCCATTTTGATATCCACTACAACAAGAGATCAGAATTGAACTCTGTTGCCCCTTTTGAACTACATGGAATGGCCATCTGTTGGGAAGATTCTCCTGTTTACTATGTTGACATTCCTAAGGATCTGCTGTGGTCTGGCAGCAGAAGTAAAGATGAAAGCCTGCGTTTGACTGCATCAGGTGATAGAACTAATGTGTTACCTCTTGATAATATGTTGGAACTGGCTAAATGTAGATGGAGCAGGATCGGTGAAATCATGGGGAAAAGAGGTGTCAGGAAATTTACTTGGATGTTGAAAAGTCAGATTCAAGCACTGAAATGCCCTGCAGTTCCAATTCAGAGATTTGGTTGCCAGAGTCTTGCGGGGAAAAGTACTTGCTTTGAAATTATAGACACCTCATTTTTATTGTTGCCACCAGTTCATATACAAGATGGCATTGACATGTGCATTGTGGCCTGGATTCTTTGGCCTGATGAGGAGAAAAATTCTAGTCCTAACCTGGAAAAGGAAGTTAAGAAAAGGTTATCCAGCAATGCTGCTGCAGCTGCAAATCGAAACGGCAGGTGGAAAAATCAGATGCGAAGAGCTGCACACAATGGTTGCTGCCGTCGAGTTGCACAAACACGAGCCTTATGTTCTGTTTTGTGGAAGTTGCTTGTTTCTGAAAACCTTACCGAGTCACTGATAAATATTGAAGTTCCATTGGTTAATATTCTTGCTGACATGGAGCTTTGGGGAGTGGGTCTTGACATGGAGGGCTGTCTGCAAGCACGTAATGTACTGGGGAAAAAGCTGAGGCATCTCGAGAAAGAAGCTTGTAAACTGGCTGGCATGACATTTTCATTATATACAGCAGCGGATATTGCTAATGTGCTGTATGGACACTTAAAGCTGCCCATACCAGAAGTGCGCAACAAGGGAAAGCAACATCCAAGTACTGACAAGCATTGCTTGGATTTACTAAGGGACGAGCATCCTATTATACCCATCATCAAAGAGCATCGTACATTGGCAAAACTCTTAAACTGTACGATGGGATCAATTTGTTCACATGCTAGGCTATCTGTAAAGACACAGAAGTACACATTACACGGTCATTGGCTCCAAACATCAACAGCTACTGGTCGGCTTTCAATGGAGGAACCTAATCTCCAGTGTGTGGAACATATGGTTGACTTCAAAATGAACAAGGAGGAAAATGGTGGCAAAACTGATGTTGATCATTACAAAATTAATGCTCGCGAATACTTTACACCTACTCAGGACAACTGGATACTGTTGACCGCAGATTATTCTCAGATAGAACTGCGGGTGATGGCCCATTTTTCTGAGGATTCCTCACTCATTGAACTCCTTAGTAAGCCCCATGGGGATGTCTTCACCATGATTGCAGCTAGATGGACTGGAAAGTCAGAAGATTGTATTAGTTCTGACATACGAGATCAGACCAAAAGGTTGGTGTATGGAATTCTCTATGGAATGGGTGCTAATAGCCTTGCTGAACAACTGGATTGCAGTCCCCAGGAAGCTTCTGAGAAAATACAAAACTTCAAAAGTTCTTTCCCTGGAGTTGCTTCTTGGCTTAATGAAGCAGTTGCATATTGCCGCAAGAAAGGATATGTGGAAACCCTCAAGGGAAGAAAACGATTTTTATCAAAAATAAAATTTGGGAATAGCAAGGAAAAGGCAAAGGCCCAGAGACAGGCTGTAAATTCGATATGTCAGGGATCCGCTGCTGATATAATTAAGATTGCAATGATAAATATTTACTCGGTAATAGTTCATGGAGCTGAAAGGCCCAGTTCAACTTCTCCACTTGCAACAAAGTTTCACAAATTGAAGGGTCGCTGCAGAATTCTATTGCAGGTACATGATGAATTGGTACTGGAAGTTGATCCTTCAGTGATAAAAGAAGCTGCTTTGCTGTTACAAACAAGCATGGAAAATGCTGTTTCACTTCTTGTTCCTTTGCATGTCAAATTGAAAGTTGGAAGAACTTGGGGTTCTTTGCAACCCTTTCAGGCGGATCAATATGAAAATCTCCTTGTTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2179

Amino Acids

239.93

Weight (kDa)

7.67

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 524 - 700 7.2e-19 DEAD/DEAH box helicase
Helicase_C PF00271 830 - 905 5.9e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 910 - 1001 2.7e-24 Helix-turn-helix domain
POLQ_helical PF21099 1025 - 1208 1.8e-51 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1214 - 1288 7e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1750 - 2162 1.5e-110 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2193
AarI CACCTGC 1 cut(s) 5034
AasI GACNNNNNNGTC 1 cut(s) 5890
AatII GACGTC 1 cut(s) 167
Acc16I TGCGCA 1 cut(s) 5393
Acc36I ACCTGC 5 cut(s) 1537, 1607, 2558, 5034, 6337
Acc65I GGTACC 1 cut(s) 2152
AccB1I GGYRCC 4 cut(s) 919, 2152, 4169, 4226
AccB7I CCANNNNNTGG 4 cut(s) 2947, 3965, 4563, 5641
AccI GTMKAC 1 cut(s) 4299
AccII CGCG 6 cut(s) 28, 720, 2574, 2658, 3255, 5724
AccIII TCCGGA 2 cut(s) 335, 3391
AcoI YGGCCR 2 cut(s) 1207, 4554
AcuI CTGAAG 7 cut(s) 945, 1544, 2841, 3096, 3756, 3900, 6366
AcvI CACGTG 1 cut(s) 842
AcyI GRCGYC 2 cut(s) 5, 164
AdeI CACNNNGTG 5 cut(s) 131, 866, 1385, 1709, 3296
AfeI AGCGCT 1 cut(s) 656
AflII CTTAAG 2 cut(s) 2369, 2440
AflIII ACRYGT 3 cut(s) 839, 2572, 4923
AgeI ACCGGT 1 cut(s) 2149
AhdI GACNNNNNGTC 1 cut(s) 472
AhlI ACTAGT 1 cut(s) 3451
AjiI CACGTC 1 cut(s) 3047
AjuI GAANNNNNNNTTGG 6 cut(s) 1360, 1392, 3351, 3383, 3585, 3617
AleI CACNNNNGTG 1 cut(s) 318
AloI GAACNNNNNNTCC 2 cut(s) 5616, 5648
Alw21I GWGCWC 4 cut(s) 916, 2915, 3301, 3856
Alw26I GTCTC 6 cut(s) 113, 538, 771, 2522, 3628, 6175
Alw44I GTGCAC 3 cut(s) 2911, 3297, 3852
AlwNI CAGNNNCTG 2 cut(s) 2108, 5603
Ama87I CYCGRG 2 cut(s) 3684, 5285
Aor13HI TCCGGA 2 cut(s) 335, 3391
Aor51HI AGCGCT 1 cut(s) 656
ApaLI GTGCAC 3 cut(s) 2911, 3297, 3852
ArsI GACNNNNNNTTYG 2 cut(s) 2680, 2712
AseI ATTAAT 2 cut(s) 1398, 5715
AsiGI ACCGGT 1 cut(s) 2149
Asp700I GAANNNNTTC 4 cut(s) 1079, 1175, 2484, 4447
Asp718I GGTACC 1 cut(s) 2152
AspLEI GCGC 5 cut(s) 30, 557, 657, 2808, 5394
AsuC2I CCSGG 1 cut(s) 4356
AsuII TTCGAA 3 cut(s) 50, 1145, 1184
AsuNHI GCTAGC 1 cut(s) 551
AvaI CYCGRG 2 cut(s) 3684, 5285
AvaII GGWCC 3 cut(s) 1267, 3511, 3931
AxyI CCTNAGG 2 cut(s) 4232, 4599
BaeGI GKGCMC 5 cut(s) 2915, 3301, 3697, 3856, 4231
BalI TGGCCA 2 cut(s) 1209, 4556
BamHI GGATCC 2 cut(s) 2464, 6209
BanI GGYRCC 4 cut(s) 919, 2152, 4169, 4226
BarI GAAGNNNNNNTAC 2 cut(s) 3111, 3143
BauI CACGAG 4 cut(s) 318, 967, 1128, 5106
BbrPI CACGTG 1 cut(s) 842
BbsI GAAGAC 4 cut(s) 294, 849, 3219, 5851
Bbv12I GWGCWC 4 cut(s) 916, 2915, 3301, 3856
BbvCI CCTCAGC 1 cut(s) 5276
BceAI ACGGC 4 cut(s) 1077, 1860, 5056, 5073
BcgI CGANNNNNNTGC 2 cut(s) 3367, 3401
BciVI GTATCC 2 cut(s) 1105, 5748
BclI TGATCA 3 cut(s) 535, 3397, 5701
BcnI CCSGG 1 cut(s) 4356
BcoDI GTCTC 6 cut(s) 113, 538, 771, 2522, 3628, 6175
BcuI ACTAGT 1 cut(s) 3451
BfmI CTRYAG 9 cut(s) 114, 645, 1320, 1815, 4146, 4203, 4806, 5022, 6331
BfoI RGCGCY 1 cut(s) 658
BfrI CTTAAG 2 cut(s) 2369, 2440
BfuAI ACCTGC 5 cut(s) 1537, 1607, 2558, 5034, 6337
BfuI GTATCC 2 cut(s) 1105, 5748
BglI GCCNNNNNGGC 1 cut(s) 3905
BmcAI AGTACT 3 cut(s) 1435, 4855, 5419
Bme18I GGWCC 3 cut(s) 1267, 3511, 3931
BmeRI GACNNNNNGTC 1 cut(s) 472
BmeT110I CYCGRG 2 cut(s) 3684, 5285
BmgBI CACGTC 1 cut(s) 3047
BmrI ACTGGG 3 cut(s) 3972, 5273, 6276
BmtI GCTAGC 1 cut(s) 555
BmuI ACTGGG 3 cut(s) 3972, 5273, 6276
BpiI GAAGAC 4 cut(s) 294, 849, 3219, 5851
BplI GAGNNNNNCTC 2 cut(s) 5804, 5836
BpmI CTGGAG 5 cut(s) 414, 1751, 2514, 5618, 6069
Bpu10I CCTNAGC 6 cut(s) 915, 2596, 2886, 4056, 4306, 5276
Bpu14I TTCGAA 3 cut(s) 50, 1145, 1184
BpuEI CTTGAG 1 cut(s) 6098
BpuMI CCSGG 1 cut(s) 4356
BsaAI YACGTR 3 cut(s) 842, 3823, 5255
BsaBI GATNNNNATC 4 cut(s) 2256, 2338, 5700, 6133
BsaHI GRCGYC 2 cut(s) 5, 164
BsaI GGTCTC 1 cut(s) 2522
BsaWI WCCGGW 5 cut(s) 8, 335, 2149, 2973, 3391
BsaXI ACNNNNNCTCC 4 cut(s) 2721, 2751, 5616, 5646
Bse118I RCCGGY 1 cut(s) 2149
Bse21I CCTNAGG 2 cut(s) 4232, 4599
Bse3DI GCAATG 7 cut(s) 598, 702, 2284, 2801, 5020, 5428, 6243
Bse8I GATNNNNATC 4 cut(s) 2256, 2338, 5700, 6133
BseAI TCCGGA 2 cut(s) 335, 3391
BseJI GATNNNNATC 4 cut(s) 2256, 2338, 5700, 6133
BseMI GCAATG 7 cut(s) 598, 702, 2284, 2801, 5020, 5428, 6243
BseMII CTCAG 9 cut(s) 929, 954, 1298, 4491, 5267, 5756, 5792, 5802, 6006
BseRI GAGGAG 2 cut(s) 9, 4973
BseSI GKGCMC 5 cut(s) 2915, 3301, 3697, 3856, 4231
BsgI GTGCAG 8 cut(s) 766, 991, 1635, 1731, 1810, 1898, 3069, 5055
Bsh1236I CGCG 6 cut(s) 28, 720, 2574, 2658, 3255, 5724
Bsh1285I CGRYCG 1 cut(s) 2150
BshNI GGYRCC 4 cut(s) 919, 2152, 4169, 4226
BshTI ACCGGT 1 cut(s) 2149
BsiEI CGRYCG 1 cut(s) 2150
BsiHKAI GWGCWC 4 cut(s) 916, 2915, 3301, 3856
BsiHKCI CYCGRG 2 cut(s) 3684, 5285
BsiSI CCGG 8 cut(s) 9, 170, 336, 2150, 2974, 3392, 3534, 4355
BslFI GGGAC 6 cut(s) 2686, 3385, 3917, 3960, 5462, 5985
BsmAI GTCTC 6 cut(s) 113, 538, 771, 2522, 3628, 6175
BsmFI GGGAC 6 cut(s) 2686, 3385, 3917, 3960, 5462, 5985
BsmI GAATGC 1 cut(s) 3535
Bso31I GGTCTC 1 cut(s) 2522
BsoBI CYCGRG 2 cut(s) 3684, 5285
Bsp119I TTCGAA 3 cut(s) 50, 1145, 1184
Bsp1286I GDGCHC 6 cut(s) 916, 2915, 3301, 3697, 3856, 4231
Bsp13I TCCGGA 2 cut(s) 335, 3391
Bsp1407I TGTACA 1 cut(s) 2287
Bsp19I CCATGG 1 cut(s) 5848
Bsp68I TCGCGA 2 cut(s) 2658, 5724
BspCNI CTCAG 9 cut(s) 928, 953, 1297, 4490, 5268, 5755, 5791, 5803, 6007
BspEI TCCGGA 2 cut(s) 335, 3391
BspFNI CGCG 6 cut(s) 28, 720, 2574, 2658, 3255, 5724
BspMAI CTGCAG 4 cut(s) 1324, 4810, 5026, 6335
BspMI ACCTGC 5 cut(s) 1537, 1607, 2558, 5034, 6337
BspOI GCTAGC 1 cut(s) 555
BspQI GCTCTTC 1 cut(s) 5059
BspT104I TTCGAA 3 cut(s) 50, 1145, 1184
BspT107I GGYRCC 4 cut(s) 919, 2152, 4169, 4226
BspTI CTTAAG 2 cut(s) 2369, 2440
BspTNI GGTCTC 1 cut(s) 2522
BsrDI GCAATG 7 cut(s) 598, 702, 2284, 2801, 5020, 5428, 6243
BsrFI RCCGGY 1 cut(s) 2149
BsrGI TGTACA 1 cut(s) 2287
BssAI RCCGGY 1 cut(s) 2149
BssNI GRCGYC 2 cut(s) 5, 164
BssSI CACGAG 4 cut(s) 318, 967, 1128, 5106
BssT1I CCWWGG 7 cut(s) 135, 405, 1541, 1783, 2239, 3040, 5848
Bst2BI CACGAG 4 cut(s) 318, 967, 1128, 5106
Bst6I CTCTTC 3 cut(s) 1863, 3876, 5059
BstACI GRCGYC 2 cut(s) 5, 164
BstAFI CTTAAG 2 cut(s) 2369, 2440
BstAPI GCANNNNNTGC 3 cut(s) 935, 2105, 3296
BstAUI TGTACA 1 cut(s) 2287
BstBAI YACGTR 3 cut(s) 842, 3823, 5255
BstBI TTCGAA 3 cut(s) 50, 1145, 1184
BstDSI CCRYGG 1 cut(s) 5848
BstEII GGTNACC 1 cut(s) 697
BstENI CCTNNNNNAGG 1 cut(s) 4985
BstFNI CGCG 6 cut(s) 28, 720, 2574, 2658, 3255, 5724
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 5 cut(s) 30, 557, 657, 2808, 5394
BstMAI GTCTC 6 cut(s) 113, 538, 771, 2522, 3628, 6175
BstMCI CGRYCG 1 cut(s) 2150
BstNSI RCATGY 4 cut(s) 2357, 4927, 5537, 6458
BstPI GGTNACC 1 cut(s) 697
BstSFI CTRYAG 9 cut(s) 114, 645, 1320, 1815, 4146, 4203, 4806, 5022, 6331
BstSLI GKGCMC 5 cut(s) 2915, 3301, 3697, 3856, 4231
BstUI CGCG 6 cut(s) 28, 720, 2574, 2658, 3255, 5724
BstV2I GAAGAC 4 cut(s) 294, 849, 3219, 5851
BstX2I RGATCY 6 cut(s) 1471, 2251, 2464, 4342, 4603, 6209
BstXI CCANNNNNNTGG 5 cut(s) 956, 1790, 2513, 3245, 5938
BstYI RGATCY 6 cut(s) 1471, 2251, 2464, 4342, 4603, 6209
Bsu36I CCTNAGG 2 cut(s) 4232, 4599
BsuI GTATCC 2 cut(s) 1105, 5748
BtgI CCRYGG 1 cut(s) 5848
BtgZI GCGATG 1 cut(s) 2882
BtrI CACGTC 1 cut(s) 3047
BtsI GCAGTG 4 cut(s) 493, 672, 1100, 1329
BtuMI TCGCGA 2 cut(s) 2658, 5724
BveI ACCTGC 5 cut(s) 1537, 1607, 2558, 5034, 6337
CaiI CAGNNNCTG 2 cut(s) 2108, 5603
CfoI GCGC 5 cut(s) 30, 557, 657, 2808, 5394
Cfr10I RCCGGY 1 cut(s) 2149
CseI GACGC 2 cut(s) 2563, 3822
CsiI ACCWGGT 2 cut(s) 862, 1381
CspAI ACCGGT 1 cut(s) 2149
CspCI CAANNNNNGTGG 4 cut(s) 1768, 1803, 3022, 3057
DraI TTTAAA 2 cut(s) 3493, 3913
DraIII CACNNNGTG 5 cut(s) 131, 866, 1385, 1709, 3296
DrdI GACNNNNNNGTC 1 cut(s) 5890
DriI GACNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 5890
EaeI YGGCCR 2 cut(s) 1207, 4554
Eam1104I CTCTTC 3 cut(s) 1863, 3876, 5059
Eam1105I GACNNNNNGTC 1 cut(s) 472
EarI CTCTTC 3 cut(s) 1863, 3876, 5059
EciI GGCGGA 1 cut(s) 6521
Eco130I CCWWGG 7 cut(s) 135, 405, 1541, 1783, 2239, 3040, 5848
Eco147I AGGCCT 1 cut(s) 3518
Eco31I GGTCTC 1 cut(s) 2522
Eco32I GATATC 2 cut(s) 4326, 4495
Eco47I GGWCC 3 cut(s) 1267, 3511, 3931
Eco47III AGCGCT 1 cut(s) 656
Eco57I CTGAAG 7 cut(s) 945, 1544, 2841, 3096, 3756, 3900, 6366
Eco72I CACGTG 1 cut(s) 842
Eco81I CCTNAGG 2 cut(s) 4232, 4599
Eco88I CYCGRG 2 cut(s) 3684, 5285
Eco91I GGTNACC 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 4985
EcoO109I RGGNCCY 3 cut(s) 2563, 3931, 4412
EcoO65I GGTNACC 1 cut(s) 697
EcoRI GAATTC 3 cut(s) 1171, 5949, 6336
EcoRV GATATC 2 cut(s) 4326, 4495
EcoT14I CCWWGG 7 cut(s) 135, 405, 1541, 1783, 2239, 3040, 5848
ErhI CCWWGG 7 cut(s) 135, 405, 1541, 1783, 2239, 3040, 5848
FaqI GGGAC 6 cut(s) 2686, 3385, 3917, 3960, 5462, 5985
FauI CCCGC 4 cut(s) 418, 2857, 4837, 5784
FauNDI CATATG 1 cut(s) 5649
FbaI TGATCA 3 cut(s) 535, 3397, 5701
FblI GTMKAC 1 cut(s) 4299
FspI TGCGCA 1 cut(s) 5393
GlaI GCGC 5 cut(s) 29, 556, 656, 2807, 5393
GsuI CTGGAG 5 cut(s) 414, 1751, 2514, 5618, 6069
HaeII RGCGCY 1 cut(s) 658
HapII CCGG 8 cut(s) 9, 170, 336, 2150, 2974, 3392, 3534, 4355
HgaI GACGC 2 cut(s) 2563, 3822
HhaI GCGC 5 cut(s) 30, 557, 657, 2808, 5394
Hin1I GRCGYC 2 cut(s) 5, 164
Hin6I GCGC 5 cut(s) 28, 555, 655, 2806, 5392
HinP1I GCGC 5 cut(s) 28, 555, 655, 2806, 5392
HincII GTYRAC 7 cut(s) 1962, 2236, 2386, 4113, 4591, 5656, 5763
HindII GTYRAC 7 cut(s) 1962, 2236, 2386, 4113, 4591, 5656, 5763
HindIII AAGCTT 5 cut(s) 192, 870, 3486, 5295, 6009
HpaII CCGG 8 cut(s) 9, 170, 336, 2150, 2974, 3392, 3534, 4355
Hpy99I CGWCG 4 cut(s) 28, 169, 1063, 5094
HpyCH4IV ACGT 5 cut(s) 164, 841, 3046, 3822, 5254
HpySE526I ACGT 5 cut(s) 164, 841, 3046, 3822, 5254
Hsp92I GRCGYC 2 cut(s) 5, 164
HspAI GCGC 5 cut(s) 28, 555, 655, 2806, 5392
Kpn2I TCCGGA 2 cut(s) 335, 3391
KpnI GGTACC 1 cut(s) 2156
Ksp22I TGATCA 3 cut(s) 535, 3397, 5701
LguI GCTCTTC 1 cut(s) 5059
LmnI GCTCC 8 cut(s) 297, 338, 1732, 4057, 4719, 5209, 5589, 6269
MabI ACCWGGT 2 cut(s) 862, 1381
MaeII ACGT 5 cut(s) 164, 841, 3046, 3822, 5254
MaeIII GTNAC 9 cut(s) 697, 1315, 1324, 3160, 3262, 3553, 4677, 5160, 6408
MfeI CAATTG 1 cut(s) 4212
MflI RGATCY 6 cut(s) 1471, 2251, 2464, 4342, 4603, 6209
MhlI GDGCHC 6 cut(s) 916, 2915, 3301, 3697, 3856, 4231
MlsI TGGCCA 2 cut(s) 1209, 4556
MluI ACGCGT 1 cut(s) 2572
MluNI TGGCCA 2 cut(s) 1209, 4556
MlyI GAGTC 3 cut(s) 2833, 4846, 5168
MmeI TCCRAC 6 cut(s) 396, 3238, 3583, 3983, 4677, 6451
Mox20I TGGCCA 2 cut(s) 1209, 4556
MroI TCCGGA 2 cut(s) 335, 3391
MroXI GAANNNNTTC 4 cut(s) 1079, 1175, 2484, 4447
MscI TGGCCA 2 cut(s) 1209, 4556
MslI CAYNNNNRTG 9 cut(s) 318, 993, 1203, 2174, 2901, 4044, 4798, 4922, 5853
Msp20I TGGCCA 2 cut(s) 1209, 4556
MspA1I CMGCKG 7 cut(s) 392, 1982, 2034, 2603, 5027, 5339, 6215
MspCI CTTAAG 2 cut(s) 2369, 2440
MspI CCGG 8 cut(s) 9, 170, 336, 2150, 2974, 3392, 3534, 4355
MunI CAATTG 1 cut(s) 4212
Mva1269I GAATGC 1 cut(s) 3535
MvnI CGCG 6 cut(s) 28, 720, 2574, 2658, 3255, 5724
NciI CCSGG 1 cut(s) 4356
NcoI CCATGG 1 cut(s) 5848
NdeI CATATG 1 cut(s) 5649
NheI GCTAGC 1 cut(s) 551
NmeAIII GCCGAG 2 cut(s) 202, 3884
NmuCI GTSAC 3 cut(s) 1324, 3262, 5160
NruI TCGCGA 2 cut(s) 2658, 5724
NsbI TGCGCA 1 cut(s) 5393
NspI RCATGY 4 cut(s) 2357, 4927, 5537, 6458
NspV TTCGAA 3 cut(s) 50, 1145, 1184
OliI CACNNNNGTG 1 cut(s) 318
PaeR7I CTCGAG 2 cut(s) 3684, 5285
PaqCI CACCTGC 1 cut(s) 5034
PceI AGGCCT 1 cut(s) 3518
PciI ACATGT 1 cut(s) 4923
PciSI GCTCTTC 1 cut(s) 5059
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 1181, 2704
PctI GAATGC 1 cut(s) 3535
PdmI GAANNNNTTC 4 cut(s) 1079, 1175, 2484, 4447
PflFI GACNNNGTC 1 cut(s) 2448
PflMI CCANNNNNTGG 4 cut(s) 2947, 3965, 4563, 5641
PfoI TCCNGGA 1 cut(s) 2940
PinAI ACCGGT 1 cut(s) 2149
PleI GAGTC 3 cut(s) 2833, 4845, 5167
PmaCI CACGTG 1 cut(s) 842
PmlI CACGTG 1 cut(s) 842
PpsI GAGTC 3 cut(s) 2833, 4845, 5167
Ppu21I YACGTR 3 cut(s) 842, 3823, 5255
PpuMI RGGWCCY 1 cut(s) 3931
PscI ACATGT 1 cut(s) 4923
PshBI ATTAAT 2 cut(s) 1398, 5715
PsiI TTATAA 1 cut(s) 2193
Psp5II RGGWCCY 1 cut(s) 3931
PspCI CACGTG 1 cut(s) 842
PspEI GGTNACC 1 cut(s) 697
PspPPI RGGWCCY 1 cut(s) 3931
PstI CTGCAG 4 cut(s) 1324, 4810, 5026, 6335
PstNI CAGNNNCTG 2 cut(s) 2108, 5603
PsuI RGATCY 6 cut(s) 1471, 2251, 2464, 4342, 4603, 6209
PsyI GACNNNGTC 1 cut(s) 2448
PvuII CAGCTG 4 cut(s) 392, 1982, 2603, 5027
RruI TCGCGA 2 cut(s) 2658, 5724
RseI CAYNNNNRTG 9 cut(s) 318, 993, 1203, 2174, 2901, 4044, 4798, 4922, 5853
SapI GCTCTTC 1 cut(s) 5059
ScaI AGTACT 3 cut(s) 1435, 4855, 5419
SchI GAGTC 3 cut(s) 2833, 4846, 5168
SduI GDGCHC 6 cut(s) 916, 2915, 3301, 3697, 3856, 4231
SexAI ACCWGGT 2 cut(s) 862, 1381
SfcI CTRYAG 9 cut(s) 114, 645, 1320, 1815, 4146, 4203, 4806, 5022, 6331
Sfr274I CTCGAG 2 cut(s) 3684, 5285
SfuI TTCGAA 3 cut(s) 50, 1145, 1184
SinI GGWCC 3 cut(s) 1267, 3511, 3931
SlaI CTCGAG 2 cut(s) 3684, 5285
SmiMI CAYNNNNRTG 9 cut(s) 318, 993, 1203, 2174, 2901, 4044, 4798, 4922, 5853
SmlI CTYRAG 5 cut(s) 2369, 2440, 3684, 5285, 6113
SmoI CTYRAG 5 cut(s) 2369, 2440, 3684, 5285, 6113
SpeI ACTAGT 1 cut(s) 3451
SseBI AGGCCT 1 cut(s) 3518
SspI AATATT 5 cut(s) 2313, 4462, 5173, 5194, 6247
StuI AGGCCT 1 cut(s) 3518
StyI CCWWGG 7 cut(s) 135, 405, 1541, 1783, 2239, 3040, 5848
TaiI ACGT 5 cut(s) 167, 844, 3049, 3825, 5257
TaqII GACCGA 1 cut(s) 2371
TatI WGTACW 9 cut(s) 1433, 1687, 2287, 2737, 3127, 4853, 5260, 5417, 5562
TauI GCSGC 1 cut(s) 6090
TseFI GTSAC 3 cut(s) 1324, 3262, 5160
Tsp45I GTSAC 3 cut(s) 1324, 3262, 5160
TspGWI ACGGA 7 cut(s) 12, 73, 284, 1259, 2293, 2627, 3232
Tth111I GACNNNGTC 1 cut(s) 2448
Van91I CCANNNNNTGG 4 cut(s) 2947, 3965, 4563, 5641
Vha464I CTTAAG 2 cut(s) 2369, 2440
VneI GTGCAC 3 cut(s) 2911, 3297, 3852
VpaK11BI GGWCC 3 cut(s) 1267, 3511, 3931
VspI ATTAAT 2 cut(s) 1398, 5715
XagI CCTNNNNNAGG 1 cut(s) 4985
XbaI TCTAGA 3 cut(s) 769, 1474, 2998
XceI RCATGY 4 cut(s) 2357, 4927, 5537, 6458
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 412
XhoI CTCGAG 2 cut(s) 3684, 5285
XmiI GTMKAC 1 cut(s) 4299
XmnI GAANNNNTTC 4 cut(s) 1079, 1175, 2484, 4447
ZraI GACGTC 1 cut(s) 165
ZrmI AGTACT 3 cut(s) 1435, 4855, 5419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.