Rroxscaffold_7G00162490

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013097
Physical Location & Seq
Reverse (-)
4972209 .. 4986379
14171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162490.1

Sequence Viewer

Length: 6546 bp
ATGGCGTCTGGTTCGCCTCCTCCGTCGCGCATCAATCAGTTTTTTGCTTCGAAGAAACGGAAGCCTGTATCACCTGCTTTGAAGTCGGGGAGAAATGAAAAGGATGTAAAAACTGTAGAAGGTTCACCTAGTGCTAAGGCCACATTGGACAGTTATCTGAGGACATCGCCGGAGAGTTATAATGTGGCGAAACCTTTGTGCAAATTTGGTGATTCAGCGGCTCGGCAAGATCAAGTTAGAAGGAATTTGGCATCCGAGATGGATAATTCTTCGAAAAATGAATGTATGCAGCACCGTTTCTCTTCTCAACTGCACACTCATGCAAGTGAGGCTTCTGGAGCAAATCAGAAGGAAATAGCTAAGGGATTAACCGAGGCGGGTGATGTTGTAGCTGAAGGTTCTTCCAAGGATTGCTCAGATTTTGCAGGAGGTGTGGAGAATGTAGAGCTGAAGAAGTTTGCAGCCAACTTTTTGTCTTTGTATTGCAGTGATGTTCAATCAAACAAAAGTTCTATGTCAGAGCTCAAAGTGAATGGTCATAAGAGATACCCTAGCCCATCTTTGCTTGATGGGGAGAGTCAGACATTTAAAAAGAGGCACTGCGGTAGTGATGAATCCTATGAAGTACGTGAAACAATTCGTTCTACTGAGAAGAGCTATGAAGCAATGCAATCTGCTTATGTTGATAAAAATAGGGCTACCATTCCTAATGAATTGCTCGAGCTTCAACAAACCTTGAAAACATGCAGTAATGCACCTAAACTGTCTCTAGACGTAGTTGATTGTTATACACCAGGCTCATTGATTAAATCATGTGTTCGTCAGACCCCCAATTCAATACGTGGTAGCTCATTTTCTTCACCTGGTGAAGCTTTTTGGAATGATGCAATTCAACTTGCTGATGGTCTATGTGCCCAGGCAGCAGAAGCAACTACAGCTTCAGATAGCCAATATCATGGTATAAACTCTTGCAATTTGAGAAACGCACACTGTGATGAAAAGTCGAAGGAAATGATGGATGAAGGTGAGCGTATGGGTAATGTTGCTGATTCAGAGCCAATGGTAAAGCATAGGAAGGACTTGCGTAAAGAAGTATCCCCACTGCCTGTTAAGCATTTTGATTTCTCTCGTGAGGACAAGATTTCGAATGAAAGTATGCCACATCATATAGATTCTTACAATTTGAAAAGCACGGCTCATGGAGGTGGCGAGCAATCTGAATCCAGTCTTATAGATCCTAGAGGTCTAAGACATGGTATTACTATGGTCCGTTGCAGCAAATCTCATGCAAATCAAGGGCAAGATGTTGATTCTGTTACTGCAGTGACCAAAATGAAAGTGGATTTGTCTAGCAAGGATAATGGTGGCCCGACATTTTCTTCACCAGGTGATGATGTCGTTAAGTTGAATGGTAAAGGTGAATCTAGCGAAGCAAGTACTCCTTCAAGTTTTCTGCCACTGAAAGATTGTCAGGATCTAGATGGTTGGCTTGCACCAGAAATATGCAGCATATATAGAAAGAAAGGAATTTCCAAGCTTTATCCTTGGCAGGTTGAGTGCCTTCAGGTTGATGGTGTATTGCAGAGAAGGAATCTTGTGTATTGTGCATCAACAAGTGCAGGTAAAAGTTTCGTTGCAGAAATTCTGATGCTGCGGCAGGTCTTGTCATCTGGAAAAATAGCTTTACTTGTACTTCCTTATGTATCACTTTGTGCAGAAAAGGCAGAACATCTGGAGCTACTTCTTGAACCACTTGGTAAGCGTGTTCGTAGTTATTATGGGAACCAAGGTGGTGGTACACTTCCTAAAGACACTACTGTAGCTGTCTGCACAATAGAAAAGGCAAACTTTTTAGTAAACAAGCTGCTGGAAGAGGGCCGTCTTTCAGAAATTGGAATTGTTGTGATAGATGAACTGCACATGGTTGGCGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACAAAACTTCGTTATGCAGCTGGTGAAGGCATTTCTGAATCAAGTAGTGGAGAAAGTTCAGGCATGAGCAGCGGTAAGGCTGACCCTGCTCATGGTTTGCAAATTGTTGGTATGAGTGCCACTATGCCAAATGTGGCAGCAGTTGCTGATTGGCTTCAAGCTGCCCTTTACCAGACAGAATTTCGACCGGTACCACTAGAAGAATACATTAAAGTGGGGAACACCATTTATAACAAGAAAATGGAGATTATTAGAACAATCCCAAAAGCAGTTGACCTTGGTGGTAAAGATCCAGATCAAGTTGTGGAACTGTGCAATGAGGTTGTACAAGAAGGTCTTTCCGTATTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCGAGGCATGTTTCAAGATTCCTTAAGAAATTTGCAGTCAACACTCGCACTGATGACAGTGAATTTAATGATGTGACATTGGCTATTGATTCCTTACGAAGGTGTCCTGCTGGTTTGGATCCAGTATTAGAAGAAACCCTACCTTCTGGAGTTGCCTACCACCATGCTGGGCTCACGGTTGAGGAAAGAGAGATTGTTGAAACCTGCTACCGTAAAGGCCATGTACGCGTCTTAACTGCCACATCTACCTTAGCAGCTGGTGTTAATCTGCCAGCTAGGAGAGTCATTTTTCGTCAGCCCCGGATTGGTCGCGATTTTATCGATGGGACAAGGTACAGGCAAATGGCTGGTCGGGCTGGTCGTACTGGCATAGATACAAAAGGAGAAAGTGTACTAATTTGCAAGCCAGAAGAGATCAAAAGGATTATGGGAGTTCTTAATGAAAGCTGTCTGCCATTGCGCTCTTGTTTATCTGAAGATATGAATGGAATGACTCATGCAATCTTAGAAGTTGTAGCAGGGGGAATTGTTCAAACTGCTAATGACATTCATCGCTATGTTAGGTGCACACTTTTAAACTCCACTAAACCTTTTCAGGATGTGGTGAAATCAGCACAGGAATCTCTCCGTTGGTTGTGCCACAAGAAATTTCTAGAATGGAATGACGACACTAAGTTATACAATACCACACCCCTTGGACGTGCAGCTTTTGGCAGTTCTCTATGTCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCAAGAGAAGGCTTTGTGCTTGCTTCTGATTTGCATCTGGTTTACCTAGTAACACCAATAAATGTTGATGTTGAGCCTGATTGGGAACTGTATTATGAACAGTTCATGGAATTGTCTGCTCTTGACCAGTCCGTTGGCAATCGCGTTGGGGTGACAGAACCATTTTTGATGCGAATGGCACATGGTGCACCTATGCGGTCATCAAACAGGATGAGAGAAAATATGAAAGCGTTGCATGGCAATTATGAAAGCCGACTTGGGACTACAAAGAGTACTGTGCTTCCGGATGATCAAACAGTTCGAGTTTGTAAACGGTTCTATGTTGCTCTCATCTTGTCAAGACTAGTTCAGGAAGTTGCTATCAATGAGGTCTGTGAAGCTTTTAAAGTGGCTAGAGGTATGGTCCAGGCCTTACAAGAGAATGCTGGAAGGTTTGCATCTATGGTAACTATGTTTTGTGAAAGGCTCGGATGGCATGATCTGGAAGGCTTGGTTGGAAAATTTCAAAACCGTGTTTCATTTGGAGTGAGAGCAGAGATTGTAGAGCTGACTACAATTCCATTTGTCAAGGGTTCTCGAGCAAGGGCACTCTATAAAGCTGGCTTGCGTACGCCTTTAGCTATTGCTGAAGCATCCATCCCTGAAATTGTCAAAGCTCTTTTCGAATCTTCATCGTGGGCAGCACAAGAAGGTTCAGCTCAAAGACGCATCCAAGTAGGAGTAGCCAAGAAGATCAAGAAGGGTGCACACAAAATTGTTCTTGAAAAGGCAGAAGAGGCCAGAGTTGCTGCCTTCTCAACTTTTAAATCACTTGGATTGGAGGTCCCACAGTTTTCTCAACCATTATTTCCAACTGCTGGTGGAAGTCCCACTATGCAAGGAGCAACAAATTCCTCTGGTGATGAAAACACTAGTAGCTTTGTTAATGACCATGTGGAGGATGCTCCTAAGCCATCTTTGGAAGGAAGGGTTCCTTCTGATAAGGTTGTATCAGGTGCGAAGTTGACAAAAACATCAGATATTGGTATGTTGGTGGCTTCAGGAGAAGTAAATTCAAGTGGTGTCATGCAAATTAAGTTTGGGGCTGAAAATTCTGTAGTACCAATTGATGGGTCTGGTGCCCTTGCTGATGAGCTAAATGCTACTGTTCATCAAACTAAAATTGCTGACTTGACCAACTCAATTAAGGTAGACAGCTTAGGTGATAGAAATAGGATATCCAGCGAGTCTTTAGATCCTAAAAAGCAGGGAAGCTGTGAAGGGGAAAATTTGTCTGTTGGTGTTAAGGGCAATGCTTGTGAAAAAGGCCCTATTCATGCAATTTACACTCCCGGAGGAATTGATTCATTTTTGAATATTTGGGAAACCACCTCAGAGTTTCATTTTGATATCCACTACAACAAAAGATCAGAATTGAACTCTGTTGCCCCCTTTGAAATACATGGAATGGCCATCTGTTGGGAAGATTCTCCTGTGTACTATGTTAACATTCCTAAGGATCTGCTGTGGTCTGGCAGCAGCAGAAAAGACGAAGGGCTGTGTTTGACTGCATCAGGTGATAGAACTAATGTGTTACCTCTTGATGATATGTTGGAGCTGGCTAAACGTAGATGGAGCAGGATTGGTGAAATCATGGGGAAAAGAGGTGTCAGGAAATTTACTTGGATGTTGAAAAGTCAGATTCAGGCACTGAAGTGCCCTGCAGTTCAAATACAGAGATTTGGTTGCCAGAGTCTTGCGAGGAAAAGTATTTGTTTTGAAATTATAGACAACTCATTTTTATTGTTGCCACCAGTTCATATACAAGATGGCATTGACATGTGCATTGTGGCCTGGATTCTCTGGCCTGATGAGGAGAAAAATTCTAATCCAAACTTGGAAAAGGAAGTTAAGAACAGGTTATCCAGTGACGCTGCAGCAGCTGCAAATCGAAATGGCAGGTGGAAAAATCAGATGCGAAGAGCTGCACACAATGGTTGCTGCCGTCGAGTTGCTCAAACACGGGCCTTATGTTCTGTTCTGTGGAAGTTGCTTGTTTCTGAAGACCTTGCTGAGTCACTTATAAATATAGAAGTTCCATTGGTTAATATTCTTGCTGACATGGAGCTTTGGGGAGTGGGTCTTGATATGGAGGGATGTCTGCAAGCACGTAATGTGCTGGGGAAAAAACTAAGACATCTTGAGAAAGAAGCTTATAAACTGGCTGGCATGACATTTTCATTATATACAGCAGCAGATATTGCTAATGTGCTTTATGGACACTTAAAGCTGTCCATACCAGAGGTGCGCAACAAGGGAAAACAACATCCAAGTACTGACAAGCATTGCTTGGATTTACTAAGGGACGAGCATCCTATTATTCCCATCATCAAAGAGCATCGTACATTGGCAAAACTCTTAAACTGTACGTTGGGATCAATTTGCTCACTGGCTAGGCTATCTGTGAAGACACAGAAGTACACATTACATGGTCATTGGCTCCAAACATCAACAGCTACTGGTCGGCTTTCAATGGAGGAACCTAATCTCCAGTGTGTGGAACATATGGTTGACTTCAAAATGAACAAGGATGAAAATGGAGGCAAAAGTGATGTTGATCATTACCAAATTAATGCTCGAGAATACTTTATACCTACTCAGGATAACTGGATACTGTTGACCGCAGATTATTCTCAGATAGAACTGCGGCTGATGGCTCATTTTTCTGAGGATTCCTCACTCATTGAACTCCTTAGTAAGCCCCATGGTGATGTCTTCACCATGATTGCAGCTAGATGGACTGGAATGTCAGAAGATTGTATTAGTTCTGATGTACGAGATCAGACCAAAAGGTTGGTGTATGGAATTCTCTACGGGATGGGTGCTAATAGCCTTGCAGAACAACTGGATTGCAGTCCCCAGGCAGCTTCTGAGAAAATACAAAACTTCAAAAGTTCTTTCCCCGGAGTTGCTTCTTGGCTCAATGAAGCAGTTGGATGTTGCCACAAGAAAGGATATGTGGAAACCCTTAAGGGAAGAAAACGATTTTTGTCAAAAATAAAATTTGGGAACAGCAAGGAAAAGTCAAAGGCGCAGAGACAGGCTGTAAATTCGATATGTCAGGGATCCGCTGCTGACATAATTAAGATTGCAATGATAAATATTTACTCCGTAATAGTTGAAGGAGCTGAAAAGCCTGATTCGACTTCTCCACTTCCAACAAAGTTTCACAAACTGAAGGGACGCTGCCGAATTCTATTGCAGGTGCATGATGAATTGGTACTTGAAGTTGATCCTTCTGTGATAAGGGAAGCTGCTTTGCTGTTACAAACAAGCATGGAAAGTGCTGTTTCGCTTCTTGTTCCTATGCATGTCAAATTGAAAGTTGGAAGAACTTGGGGTTCTTTGCAACCCTTTCAGGCGGACCAATATGAAGATCTGCCTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2181

Amino Acids

240.42

Weight (kDa)

7.0

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 525 - 701 1.4e-18 DEAD/DEAH box helicase
Helicase_C PF00271 831 - 906 1.2e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 911 - 1002 9.7e-25 Helix-turn-helix domain
POLQ_helical PF21099 1026 - 1210 9.9e-53 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1216 - 1290 6e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1753 - 2165 1.7e-109 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 180, 2196, 5177, 5310
AarI CACCTGC 3 cut(s) 82, 5043, 6346
AasI GACNNNNNNGTC 1 cut(s) 5899
Acc16I TGCGCA 1 cut(s) 5402
Acc36I ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5043, 6346
Acc65I GGTACC 1 cut(s) 2155
AccB1I GGYRCC 2 cut(s) 2155, 4232
AccB7I CCANNNNNTGG 4 cut(s) 3971, 4223, 4572, 5650
AccI GTMKAC 1 cut(s) 4305
AccII CGCG 4 cut(s) 28, 2577, 2661, 3258
AccIII TCCGGA 1 cut(s) 3397
AcoI YGGCCR 1 cut(s) 4563
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 6 cut(s) 131, 866, 992, 1388, 1712, 3299
AfiI CCNNNNNNNGG 9 cut(s) 1940, 2057, 2448, 2654, 3971, 4051, 4223, 4572, 5650
AflII CTTAAG 2 cut(s) 2372, 6122
AflIII ACRYGT 2 cut(s) 2575, 4932
AgeI ACCGGT 1 cut(s) 2152
AhlI ACTAGT 2 cut(s) 3457, 4025
AjiI CACGTC 1 cut(s) 3050
AjnI CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4946, 6012
AjuI GAANNNNNNNTTGG 4 cut(s) 3354, 3386, 3591, 3623
AleI CACNNNNGTG 1 cut(s) 4807
AloI GAACNNNNNNTCC 4 cut(s) 5000, 5032, 5625, 5657
Alw21I GWGCWC 4 cut(s) 525, 2918, 3304, 3862
Alw26I GTCTC 2 cut(s) 771, 6184
Alw44I GTGCAC 3 cut(s) 2914, 3300, 3858
AlwNI CAGNNNCTG 5 cut(s) 2111, 4804, 5036, 5612, 6023
Ama87I CYCGRG 3 cut(s) 719, 3690, 5730
Aor13HI TCCGGA 1 cut(s) 3397
ApaLI GTGCAC 3 cut(s) 2914, 3300, 3858
ArsI GACNNNNNNTTYG 2 cut(s) 2683, 2715
AseI ATTAAT 2 cut(s) 2312, 5724
AsiGI ACCGGT 1 cut(s) 2152
Asp700I GAANNNNTTC 2 cut(s) 636, 4456
Asp718I GGTACC 1 cut(s) 2155
AspLEI GCGC 4 cut(s) 30, 2811, 5403, 6187
AspS9I GGNCC 8 cut(s) 1267, 1367, 1876, 3517, 3937, 4421, 5118, 6517
AsuC2I CCSGG 3 cut(s) 2650, 4446, 6057
AsuII TTCGAA 4 cut(s) 50, 272, 1145, 3777
AvaI CYCGRG 3 cut(s) 719, 3690, 5730
AvaII GGWCC 4 cut(s) 1267, 3517, 3937, 6517
AxyI CCTNAGG 1 cut(s) 4608
BaeGI GKGCMC 7 cut(s) 916, 2918, 3304, 3703, 3862, 4237, 4814
BalI TGGCCA 1 cut(s) 4565
BamHI GGATCC 2 cut(s) 2467, 6218
BanI GGYRCC 2 cut(s) 2155, 4232
BanII GRGCYC 2 cut(s) 525, 2523
BarI GAAGNNNNNNTAC 2 cut(s) 2472, 2504
BauI CACGAG 1 cut(s) 1128
BbsI GAAGAC 3 cut(s) 5163, 5567, 5860
Bbv12I GWGCWC 4 cut(s) 525, 2918, 3304, 3862
BceAI ACGGC 3 cut(s) 1209, 1863, 5082
BciT130I CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4948, 6014
BciVI GTATCC 2 cut(s) 1105, 5757
BclI TGATCA 2 cut(s) 3403, 5710
BcnI CCSGG 3 cut(s) 2650, 4446, 6057
BcoDI GTCTC 2 cut(s) 771, 6184
BcuI ACTAGT 2 cut(s) 3457, 4025
BfmI CTRYAG 7 cut(s) 114, 933, 1320, 1818, 4209, 4815, 5028
BfrI CTTAAG 2 cut(s) 2372, 6122
BfuAI ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5043, 6346
BfuI GTATCC 2 cut(s) 1105, 5757
BglII AGATCT 1 cut(s) 6529
BmcAI AGTACT 3 cut(s) 1438, 3388, 5428
Bme18I GGWCC 4 cut(s) 1267, 3517, 3937, 6517
BmeT110I CYCGRG 3 cut(s) 719, 3690, 5730
BmgBI CACGTC 1 cut(s) 3050
BmgT120I GGNCC 8 cut(s) 1267, 1367, 1876, 3517, 3937, 4421, 5118, 6517
BmiI GGNNCC 9 cut(s) 1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
BpiI GAAGAC 3 cut(s) 5163, 5567, 5860
BplI GAGNNNNNCTC 2 cut(s) 5813, 5845
BpmI CTGGAG 4 cut(s) 357, 1754, 2517, 5627
Bpu10I CCTNAGC 5 cut(s) 135, 360, 2599, 4062, 4312
Bpu14I TTCGAA 4 cut(s) 50, 272, 1145, 3777
BpuEI CTTGAG 1 cut(s) 5315
BpuMI CCSGG 3 cut(s) 2650, 4446, 6057
Bsa29I ATCGAT 1 cut(s) 2670
BsaAI YACGTR 3 cut(s) 629, 842, 5264
BsaBI GATNNNNATC 4 cut(s) 2259, 2341, 3147, 5709
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 2 cut(s) 2152, 3397
BsaXI ACNNNNNCTCC 4 cut(s) 2724, 2754, 5625, 5655
Bsc4I CCNNNNNNNGG 9 cut(s) 1940, 2057, 2448, 2654, 3971, 4051, 4223, 4572, 5650
Bse118I RCCGGY 1 cut(s) 2152
Bse21I CCTNAGG 1 cut(s) 4608
Bse3DI GCAATG 6 cut(s) 672, 2287, 2804, 4411, 5437, 6252
Bse8I GATNNNNATC 4 cut(s) 2259, 2341, 3147, 5709
BseAI TCCGGA 1 cut(s) 3397
BseBI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4948, 6014
BseCI ATCGAT 1 cut(s) 2670
BseJI GATNNNNATC 4 cut(s) 2259, 2341, 3147, 5709
BseLI CCNNNNNNNGG 9 cut(s) 1940, 2057, 2448, 2654, 3971, 4051, 4223, 4572, 5650
BseMI GCAATG 6 cut(s) 672, 2287, 2804, 4411, 5437, 6252
BseMII CTCAG 9 cut(s) 149, 429, 639, 4500, 5157, 5765, 5801, 5811, 6015
BseRI GAGGAG 2 cut(s) 9, 4982
BseSI GKGCMC 7 cut(s) 916, 2918, 3304, 3703, 3862, 4237, 4814
BseYI CCCAGC 2 cut(s) 2516, 5272
BsgI GTGCAG 7 cut(s) 296, 1638, 1734, 1813, 1901, 3072, 5064
Bsh1236I CGCG 4 cut(s) 28, 2577, 2661, 3258
Bsh1285I CGRYCG 1 cut(s) 2153
BshNI GGYRCC 2 cut(s) 2155, 4232
BshTI ACCGGT 1 cut(s) 2152
BshVI ATCGAT 1 cut(s) 2670
BsiEI CGRYCG 1 cut(s) 2153
BsiHKAI GWGCWC 4 cut(s) 525, 2918, 3304, 3862
BsiHKCI CYCGRG 3 cut(s) 719, 3690, 5730
BsiSI CCGG 6 cut(s) 170, 2153, 2650, 3398, 4446, 6057
BsiWI CGTACG 1 cut(s) 3722
BslFI GGGAC 7 cut(s) 2689, 3388, 3923, 3966, 5471, 5994, 6348
BslI CCNNNNNNNGG 9 cut(s) 1940, 2057, 2448, 2654, 3971, 4051, 4223, 4572, 5650
BsmAI GTCTC 2 cut(s) 771, 6184
BsmFI GGGAC 7 cut(s) 2689, 3388, 3923, 3966, 5471, 5994, 6348
BsmI GAATGC 1 cut(s) 3541
BsoBI CYCGRG 3 cut(s) 719, 3690, 5730
Bsp119I TTCGAA 4 cut(s) 50, 272, 1145, 3777
Bsp1286I GDGCHC 9 cut(s) 525, 916, 2523, 2918, 3304, 3703, 3862, 4237, 4814
Bsp13I TCCGGA 1 cut(s) 3397
Bsp1407I TGTACA 1 cut(s) 2290
Bsp19I CCATGG 1 cut(s) 5857
Bsp68I TCGCGA 1 cut(s) 2661
BspCNI CTCAG 9 cut(s) 150, 428, 640, 4499, 5158, 5764, 5800, 5812, 6016
BspDI ATCGAT 1 cut(s) 2670
BspEI TCCGGA 1 cut(s) 3397
BspFNI CGCG 4 cut(s) 28, 2577, 2661, 3258
BspLI GGNNCC 9 cut(s) 1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
BspMAI CTGCAG 3 cut(s) 1324, 4819, 5032
BspMI ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5043, 6346
BspQI GCTCTTC 2 cut(s) 647, 5068
BspT104I TTCGAA 4 cut(s) 50, 272, 1145, 3777
BspT107I GGYRCC 2 cut(s) 2155, 4232
BspTI CTTAAG 2 cut(s) 2372, 6122
BsrDI GCAATG 6 cut(s) 672, 2287, 2804, 4411, 5437, 6252
BsrFI RCCGGY 1 cut(s) 2152
BsrGI TGTACA 1 cut(s) 2290
BssAI RCCGGY 1 cut(s) 2152
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1128
BssT1I CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5857
Bst2BI CACGAG 1 cut(s) 1128
Bst2UI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4948, 6014
Bst6I CTCTTC 6 cut(s) 307, 647, 1866, 2754, 3882, 5068
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 2372, 6122
BstAPI GCANNNNNTGC 4 cut(s) 2108, 3299, 5036, 5354
BstAUI TGTACA 1 cut(s) 2290
BstBAI YACGTR 3 cut(s) 629, 842, 5264
BstBI TTCGAA 4 cut(s) 50, 272, 1145, 3777
BstDSI CCRYGG 1 cut(s) 5857
BstENI CCTNNNNNAGG 1 cut(s) 2446
BstFNI CGCG 4 cut(s) 28, 2577, 2661, 3258
BstHHI GCGC 4 cut(s) 30, 2811, 5403, 6187
BstMAI GTCTC 2 cut(s) 771, 6184
BstMCI CGRYCG 1 cut(s) 2153
BstNI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4948, 6014
BstNSI RCATGY 4 cut(s) 747, 2360, 4936, 6467
BstSFI CTRYAG 7 cut(s) 114, 933, 1320, 1818, 4209, 4815, 5028
BstSLI GKGCMC 7 cut(s) 916, 2918, 3304, 3703, 3862, 4237, 4814
BstUI CGCG 4 cut(s) 28, 2577, 2661, 3258
BstV2I GAAGAC 3 cut(s) 5163, 5567, 5860
BstX2I RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4348, 4612, 6218, 6529
BstXI CCANNNNNNTGG 5 cut(s) 956, 1793, 2516, 3248, 5947
BstYI RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4348, 4612, 6218, 6529
Bsu15I ATCGAT 1 cut(s) 2670
Bsu36I CCTNAGG 1 cut(s) 4608
BsuI GTATCC 2 cut(s) 1105, 5757
BsuTUI ATCGAT 1 cut(s) 2670
BtgI CCRYGG 1 cut(s) 5857
BtgZI GCGATG 2 cut(s) 150, 2885
BtrI CACGTC 1 cut(s) 3050
BtsI GCAGTG 4 cut(s) 493, 598, 1100, 1329
BtuMI TCGCGA 1 cut(s) 2661
BveI ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5043, 6346
CaiI CAGNNNCTG 5 cut(s) 2111, 4804, 5036, 5612, 6023
CfoI GCGC 4 cut(s) 30, 2811, 5403, 6187
Cfr10I RCCGGY 1 cut(s) 2152
Cfr13I GGNCC 8 cut(s) 1267, 1367, 1876, 3517, 3937, 4421, 5118, 6517
ClaI ATCGAT 1 cut(s) 2670
CseI GACGC 4 cut(s) 2566, 3828, 5033, 6345
CsiI ACCWGGT 2 cut(s) 862, 1384
CspAI ACCGGT 1 cut(s) 2152
CspCI CAANNNNNGTGG 2 cut(s) 3025, 3060
DraI TTTAAA 4 cut(s) 589, 2925, 3499, 3919
DraIII CACNNNGTG 6 cut(s) 131, 866, 992, 1388, 1712, 3299
DrdI GACNNNNNNGTC 1 cut(s) 5899
DseDI GACNNNNNNGTC 1 cut(s) 5899
EaeI YGGCCR 1 cut(s) 4563
Eam1104I CTCTTC 6 cut(s) 307, 647, 1866, 2754, 3882, 5068
EarI CTCTTC 6 cut(s) 307, 647, 1866, 2754, 3882, 5068
EciI GGCGGA 1 cut(s) 6530
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5857
Eco147I AGGCCT 1 cut(s) 3524
Eco24I GRGCYC 2 cut(s) 525, 2523
Eco32I GATATC 2 cut(s) 4332, 4504
Eco47I GGWCC 4 cut(s) 1267, 3517, 3937, 6517
Eco53kI GAGCTC 1 cut(s) 523
Eco81I CCTNAGG 1 cut(s) 4608
Eco88I CYCGRG 3 cut(s) 719, 3690, 5730
EcoICRI GAGCTC 1 cut(s) 523
EcoNI CCTNNNNNAGG 1 cut(s) 2446
EcoO109I RGGNCCY 2 cut(s) 3937, 4421
EcoRI GAATTC 2 cut(s) 5958, 6345
EcoRII CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4946, 6012
EcoRV GATATC 2 cut(s) 4332, 4504
EcoT14I CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5857
EcoT22I ATGCAT 1 cut(s) 6465
EcoT38I GRGCYC 2 cut(s) 525, 2523
ErhI CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5857
FaqI GGGAC 7 cut(s) 2689, 3388, 3923, 3966, 5471, 5994, 6348
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 5658
FbaI TGATCA 2 cut(s) 3403, 5710
FblI GTMKAC 1 cut(s) 4305
FriOI GRGCYC 2 cut(s) 525, 2523
FspI TGCGCA 1 cut(s) 5402
GlaI GCGC 4 cut(s) 29, 2810, 5402, 6186
GsaI CCCAGC 2 cut(s) 2520, 5276
GsuI CTGGAG 4 cut(s) 357, 1754, 2517, 5627
HapII CCGG 6 cut(s) 170, 2153, 2650, 3398, 4446, 6057
HgaI GACGC 4 cut(s) 2566, 3828, 5033, 6345
HhaI GCGC 4 cut(s) 30, 2811, 5403, 6187
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 4 cut(s) 28, 2809, 5401, 6185
HinP1I GCGC 4 cut(s) 28, 2809, 5401, 6185
HincII GTYRAC 7 cut(s) 1965, 2239, 2389, 4119, 4600, 5665, 5772
HindII GTYRAC 7 cut(s) 1965, 2239, 2389, 4119, 4600, 5665, 5772
HindIII AAGCTT 4 cut(s) 870, 1535, 3492, 5304
HpaI GTTAAC 1 cut(s) 4600
HpaII CCGG 6 cut(s) 170, 2153, 2650, 3398, 4446, 6057
Hpy99I CGWCG 2 cut(s) 28, 5103
HpyCH4IV ACGT 7 cut(s) 628, 774, 841, 3049, 4720, 5263, 5522
HpySE526I ACGT 7 cut(s) 628, 774, 841, 3049, 4720, 5263, 5522
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 4 cut(s) 28, 2809, 5401, 6185
Kpn2I TCCGGA 1 cut(s) 3397
KpnI GGTACC 1 cut(s) 2159
Ksp22I TGATCA 2 cut(s) 3403, 5710
KspAI GTTAAC 1 cut(s) 4600
LguI GCTCTTC 2 cut(s) 647, 5068
LmnI GCTCC 9 cut(s) 338, 1735, 3995, 4063, 4708, 4728, 5218, 5598, 6278
MabI ACCWGGT 2 cut(s) 862, 1384
MaeII ACGT 7 cut(s) 628, 774, 841, 3049, 4720, 5263, 5522
MfeI CAATTG 1 cut(s) 4218
MflI RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4348, 4612, 6218, 6529
MhlI GDGCHC 9 cut(s) 525, 916, 2523, 2918, 3304, 3703, 3862, 4237, 4814
MlsI TGGCCA 1 cut(s) 4565
MluI ACGCGT 1 cut(s) 2575
MluNI TGGCCA 1 cut(s) 4565
MlyI GAGTC 6 cut(s) 586, 2640, 2836, 4349, 4855, 5177
MmeI TCCRAC 6 cut(s) 3589, 3989, 4686, 6067, 6335, 6460
Mox20I TGGCCA 1 cut(s) 4565
Mph1103I ATGCAT 1 cut(s) 6465
MroI TCCGGA 1 cut(s) 3397
MroXI GAANNNNTTC 2 cut(s) 636, 4456
MscI TGGCCA 1 cut(s) 4565
MslI CAYNNNNRTG 9 cut(s) 318, 324, 993, 1203, 2177, 2904, 4807, 4931, 5862
Msp20I TGGCCA 1 cut(s) 4565
MspA1I CMGCKG 6 cut(s) 218, 1985, 2037, 2606, 5036, 6224
MspCI CTTAAG 2 cut(s) 2372, 6122
MspI CCGG 6 cut(s) 170, 2153, 2650, 3398, 4446, 6057
MunI CAATTG 1 cut(s) 4218
Mva1269I GAATGC 1 cut(s) 3541
MvaI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4948, 6014
MvnI CGCG 4 cut(s) 28, 2577, 2661, 3258
NciI CCSGG 3 cut(s) 2650, 4446, 6057
NcoI CCATGG 1 cut(s) 5857
NdeI CATATG 1 cut(s) 5658
NlaIV GGNNCC 9 cut(s) 1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 5 cut(s) 1324, 2422, 3265, 5021, 5169
NruI TCGCGA 1 cut(s) 2661
NsbI TGCGCA 1 cut(s) 5402
NsiI ATGCAT 1 cut(s) 6465
NspI RCATGY 4 cut(s) 747, 2360, 4936, 6467
NspV TTCGAA 4 cut(s) 50, 272, 1145, 3777
OliI CACNNNNGTG 1 cut(s) 4807
PaeR7I CTCGAG 3 cut(s) 719, 3690, 5730
PaqCI CACCTGC 3 cut(s) 82, 5043, 6346
PceI AGGCCT 1 cut(s) 3524
PciI ACATGT 1 cut(s) 4932
PciSI GCTCTTC 2 cut(s) 647, 5068
PcsI WCGNNNNNNNCGW 2 cut(s) 20, 2707
PctI GAATGC 1 cut(s) 3541
PdmI GAANNNNTTC 2 cut(s) 636, 4456
Pfl23II CGTACG 1 cut(s) 3722
PflMI CCANNNNNTGG 4 cut(s) 3971, 4223, 4572, 5650
PfoI TCCNGGA 1 cut(s) 4444
PinAI ACCGGT 1 cut(s) 2152
PleI GAGTC 6 cut(s) 585, 2639, 2836, 4348, 4854, 5176
PpsI GAGTC 6 cut(s) 585, 2639, 2836, 4348, 4854, 5176
Ppu21I YACGTR 3 cut(s) 629, 842, 5264
PpuMI RGGWCCY 1 cut(s) 3937
PscI ACATGT 1 cut(s) 4932
PshBI ATTAAT 2 cut(s) 2312, 5724
PsiI TTATAA 4 cut(s) 180, 2196, 5177, 5310
Psp124BI GAGCTC 1 cut(s) 525
Psp5II RGGWCCY 1 cut(s) 3937
Psp6I CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4946, 6012
PspFI CCCAGC 2 cut(s) 2516, 5272
PspGI CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4946, 6012
PspLI CGTACG 1 cut(s) 3722
PspN4I GGNNCC 9 cut(s) 1784, 2157, 2469, 3939, 4086, 4234, 5594, 5634, 6220
PspPI GGNCC 8 cut(s) 1267, 1367, 1876, 3517, 3937, 4421, 5118, 6517
PspPPI RGGWCCY 1 cut(s) 3937
PspXI VCTCGAGB 1 cut(s) 719
PstI CTGCAG 3 cut(s) 1324, 4819, 5032
PstNI CAGNNNCTG 5 cut(s) 2111, 4804, 5036, 5612, 6023
PsuI RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4348, 4612, 6218, 6529
PvuII CAGCTG 3 cut(s) 1985, 2606, 5036
RruI TCGCGA 1 cut(s) 2661
RseI CAYNNNNRTG 9 cut(s) 318, 324, 993, 1203, 2177, 2904, 4807, 4931, 5862
SacI GAGCTC 1 cut(s) 525
SapI GCTCTTC 2 cut(s) 647, 5068
Sau96I GGNCC 8 cut(s) 1267, 1367, 1876, 3517, 3937, 4421, 5118, 6517
ScaI AGTACT 3 cut(s) 1438, 3388, 5428
SchI GAGTC 6 cut(s) 586, 2640, 2836, 4349, 4855, 5177
SduI GDGCHC 9 cut(s) 525, 916, 2523, 2918, 3304, 3703, 3862, 4237, 4814
SexAI ACCWGGT 2 cut(s) 862, 1384
SfcI CTRYAG 7 cut(s) 114, 933, 1320, 1818, 4209, 4815, 5028
Sfr274I CTCGAG 3 cut(s) 719, 3690, 5730
SfuI TTCGAA 4 cut(s) 50, 272, 1145, 3777
SinI GGWCC 4 cut(s) 1267, 3517, 3937, 6517
SlaI CTCGAG 3 cut(s) 719, 3690, 5730
SmiMI CAYNNNNRTG 9 cut(s) 318, 324, 993, 1203, 2177, 2904, 4807, 4931, 5862
SmlI CTYRAG 6 cut(s) 719, 2372, 3690, 5294, 5730, 6122
SmoI CTYRAG 6 cut(s) 719, 2372, 3690, 5294, 5730, 6122
SpeI ACTAGT 2 cut(s) 3457, 4025
SseBI AGGCCT 1 cut(s) 3524
SspI AATATT 4 cut(s) 2316, 4471, 5203, 6256
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 3524
StyI CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5857
TaiI ACGT 7 cut(s) 631, 777, 844, 3052, 4723, 5266, 5525
TatI WGTACW 8 cut(s) 1436, 1690, 2290, 2740, 3386, 4590, 5426, 5571
TauI GCSGC 3 cut(s) 221, 1657, 5803
TseFI GTSAC 5 cut(s) 1324, 2422, 3265, 5021, 5169
Tsp45I GTSAC 5 cut(s) 1324, 2422, 3265, 5021, 5169
TspGWI ACGGA 7 cut(s) 12, 73, 1259, 2296, 2966, 3235, 6253
Van91I CCANNNNNTGG 4 cut(s) 3971, 4223, 4572, 5650
Vha464I CTTAAG 2 cut(s) 2372, 6122
VneI GTGCAC 3 cut(s) 2914, 3300, 3858
VpaK11BI GGWCC 4 cut(s) 1267, 3517, 3937, 6517
VspI ATTAAT 2 cut(s) 2312, 5724
XagI CCTNNNNNAGG 1 cut(s) 2446
XbaI TCTAGA 3 cut(s) 769, 1477, 3001
XceI RCATGY 4 cut(s) 747, 2360, 4936, 6467
XcmI CCANNNNNNNNNTGG 1 cut(s) 1336
XhoI CTCGAG 3 cut(s) 719, 3690, 5730
XmiI GTMKAC 1 cut(s) 4305
XmnI GAANNNNTTC 2 cut(s) 636, 4456
ZrmI AGTACT 3 cut(s) 1438, 3388, 5428
Zsp2I ATGCAT 1 cut(s) 6465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.