RLG00000010870

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013097
Physical Location & Seq
Reverse (-)
4915796 .. 4929122
13327 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010870

Sequence Viewer

Length: 6543 bp
ATGGCGTCTGGTTCGCCTCCTCAGTCGCGCATCAATCAGTTTTTTGCTTCGAAGAAACGGAAGCCTGTATCACCTGCTTTGAAGTCTGGGAGAAATGAAAAGGACGTAAAAACTGTAGAAGGTTCACCTAGTGCTAAGCCCACATTGGACAGTTATTTGCGGACATCGCCGGAGAGTTATAATGTGGCGAAACCTTTGTGCAAATTTGGTGATTCAGCGGCTAGGCAAGATCAAGTTAGAAGGAATTTGGCATCCGAGATGGATAATTCTTCGAAAAATGAATGTATGCAGCACCGTTTCTCTTCTCAACTGCACACTCATGCAAGTGAGGCTTCTGGAGAAAATCAGAAGGAAATAGCTAAGGGATTAACCGAGGCGGGTGATGTTGCAGCTGGAGGTTCTTCCAAGGATTACTCAGATATTGCAGGAGGTGTGGAGAATGTAGAGCTGAAGAAGTTTGCAGCCAACTTTTTGTCTTTGTATTGCAGTGATGTTCAATCAAACAAAAATTCTATGTCAGAGGTGAAAGTGAATGGTCATAAGAGACACCCTAGCCCATCTTTGCTTGATGAGGAGAGTAAGACATTTAAAAAGAGGCATTGCGGTAGTGATAAATCCTATGAAGAACGTGAAACAATTGGTTCAACTGAGAAGAGCTATGAAGCAATGCAATCTTCTTGTGTTGATAAAAATAGGGTTACCATTCCTAATGAATTGCTTGAGCTTCAACAAACCTTGAAAACATGCAGTAATACACCTAAATTGTCTCTAGACGTAGTTGATTGTTATACACCAGGCTCATTGATTAAATCATGTGTTCGTCAGACCCCCAATTCAATACATGGTAGCTCATTTTCTTCACCTGGTGAAGCTTTTTGGAGTGATGCAATTCAACTTGCTGATGGTCTATGTGCCCAGGCAGCAGAAGCAACTACAGCTTCAGATAGACAAAATCATGGTATAAACTCTTGCAATTTGAGAAATGCACACTGTGATGACAAGTCAAAGGAAATGATGGATGAAGGTGAGCGTATGGGTAATGGTGCTGATTCAGAGCCAATGGTAAAGCATAGGAAGGACTTGCGTAAAGAAGTATCCCCACTGCCTGTTAAGCATTTTGATTTCTCTCGTGAGGACAAGATTTCGAATGAAAGTATGCCACATCATATAGATTCTTACAATTTGAAAAACACTGCTCATGGAGGTGGCAAGCAATCTGAATCCAGTCTTATAGATCCTAGAGGTCTAAGACATGGTATTACTATGGTCCGTTGCAGCAAATCTCAGGCAAAGCAAGGGCAAGATGCTGATTCTGTTACTGCTGTGGCCAAAATGAAAGTGGATTTGTCTAGCAAGGATAATGGTGGCCCGACATTTTCTTCACCAGGTGATGATGTCGTTAAGTTGAATGGTAAAGGTGAATCTAGCGAATCAAGTACTCCTTCAAGTTTTCTGCCACTGAAAGATTGTCTGGATCTAGATGGTTGGCTTGCACCAGAAATATGCAGCATATATAGAAAGAAAGGAATTTCCAAGCTTTATCCTTGGCAGGTTGAGTGCCTTCAGGTTGATGGTGTATTGCAGAGAAGGAATCTTGTGTATTGTGCATCAACGAGTGCAGGTAAAAGTTTCGTTGCAGAAATTCTGATGCTGCGGCAGGTCTTGTCATCTGGAAAAATAGCTTTACTTGTACTTCCTTATGTATCACTTTGTGCAGAAAAGGCAGAACATCTGGAGCTACTTCTTGAACCACTTGGTAAGCGTGTTCGTAGTTATTATGGGAACCAAGGTGGTGGTACACTTCCTAAAGACACTACTGTAGCTGTCTGCACAATAGAAAAGGCAAATTTTTTAGTAAACAAGCTGCTGGAAGAGGGCCGTCTTTCAGAAATTGGAATTGTTGTGATAGATGAACTGCACATGGTTGGCGATCCAAGTAGGGGTTATCTTCTGGAACTTCTGTTGACAAAACTTCGTTATGCAGCTGGTGAAGGCATTTCTGAATCAAGTAGTGGAGAAAGTTCAGGCATGAGCAGCGGTAAGGCTGACCCTGCTCATGGTTTGCAAATTGTTGGTATGAGTGCCACTATGCCAAATGTGGCAGCAGTTGCTGATTGGCTTCAAGCTGCCCTTTACCAGACAGAATTTCGACCGGTACCACTAGAAGAATACATTAAAGTGGGGAACACCATTTATAACAAGAAAATGGAGATTATTAGAACAATCCCAAAAGCAGTTGACCTTGGTGGTAAAGATCCAGATCATGTTGTGGAACTGTGCAATGAGGTTGTACAAGAAGGTCTTTCCGTGTTAATATTTTGCTCTAGTCGAAAAGGATGTGAATCAACAGCGAGGCATGTTTCAAGATTCCTTAAGAAATTTGCAGTCAACACTCGCACTGATGACAGTGAATTTAATAATGTGACATTGGCTATTGATTCCTTACGAAGGTGTCCTGCTGGTTTGGATCCAGTATTAGAAGAAACCCTACCTTCTGGAGTTGCCTACCACCATGCTGGGCTCACGGTTGAGGAAAGAGAGATTGTTGAAACCTGCTACCGTAAAGGCCATGTACGCGTCTTAACTGCCACATCTACCTTAGCAGCTGGTGTTAATCTGCCAGCTAGGAGAGTCATTTTTCGTCAGCCCCGGATTGGTCGCGATTTTATTGATGGGACAAGGTACAGGCAAATGGCTGGTCGGGCTGGTCGTACTGGCATAGATACAAAAGGAGAAAGTGTACTAATTTGCAAGCCAGAAGAGATCAAAAGGATTATGGGAGTTCTTAATGAAAGCTGTCTGCCATTGCGCTCTTGTTTATCTGAAGATATGAATGGAATGACTCATGCAATCTTAGAAGTTGTAGCAGGGGGAATTGTTCAAACTGCTAATGACATTCATCGCTATGTTAGGTGCACACTTTTAAACTCCACTAAACCTTTTCAGGATGTGGTGAAATCAGCACAGGAATCTCTCCGCTGGTTGTGCCACAAGAAATTTCTAGAATGGAATGACGACACTAAGTTATACAATACCACACCCCTTGGACGTGCAGCTTTTGGCAGTTCTCTATGTCCTGAAGAATCACTTATTGTGCTGGATGATCTTTCAAGAGCAAGAGAAGGCTTTGTGCTTGCTTCTGATTTGCATCTGGTTTACCTAGTAACACCAATAAATGTTGATGTTGAGCCTGATTGGGAACTGTATTATGAACAGTTCATGGAATTGTCTGCTCTTGACCAGTCCGTTGGCAATCGCGTTGGGGTGACAGAACCATTTTTGATGCGAATGGCACATGGTGCACCTATGCGGTTATCAAACAGGATGAGAGAAAATACGAAAGTGTTGCATGGCAATTATGAAAGCCGACTTGGGACTACAAAGAGTACTGTGCTTCCGGATGATCAAACAGTTCGAGTTTGTAAACGGTTCTATGTTGCTCTCATCTTGTCAAGACTAGTTCAGGAAGTTGCTATCAATGAGGTCTGTGAAGCTTTTAAAGTGGCTAGAGGTATGGTCCAGGGCTTACAAGAGAATGCTGGAAGGTTTGCATCTATGGTAACTATGTTTTGTGAAAGGCTCGGATGGCATGATCTGGAAGGCTTGGTTGGAAAATTTCAAAACCGTGTTTCATTTGGAGTGAGAGCAGAGATTGTAGAGCTGACTACAATTCCATTTGTCAAGGGTTCTCGAGCAAGGGCACTCTATAAAGCTGGCTTGCGTACGCCTTTAGCTATTGCTGAAGCATCCATCCCTGAAATTGTCAAAGCTCTTTTTGAATCTTCATCGTGGGCAGCACAAGGTTCAGCTCAAAGACGCATCCAAGTAGGAGTAGCCAAGAAGATCAAGAAGGGTGCACACAAAATTGTTCTTGAAAAGGCGGAAGAGGCCAGAGTTGCTGCCTTTTCAGCTTTTAAATCACTTGGATTGGAGGTCCCACAGTTTTCTCAACCATTATTTCCAACTGCTGGTGGAAGTCCCACTATGCAAGGAGCAACAAATTCCTCTGGTGATGAAAACACTAGTAGCTTTGTTAATGCCCACGTGGAGGATGCTCCTAAGCCATCTTTGGAAGGAAGGGTTCCTTCTGATAAGGTTGTATCAGGTGCGAAGTTGACAAAAACATCAGATATTGGTATGTTGGTGGCTTCAGGAGAAGTAAATTCAAGTGGTGTCATGCAAATTAAGTTTGGGGATGAAAATTCTGTAGTACCAATTGATGGGTCTGGTGCCCTTGCTGATGAGCTAAATGCTACTGTTCATCAAACTAAAATTGCTGACTTGACCAACTCGATTAAGGTAGACAGCTTAGGTGATAGAAATAGGATATCCAGCGAGTCTTTAGATCCTAAAAAGCAGGGACGCTGTGAAGGGGAAAATTTGTCTGTTGGTGTTAAGGGCAATGCTTGTGAAAAAGGCCCTATTCATGCAATTTATACTCCCGGTGGAATAGATTCATTTTTGAATATTTGGGAAACCACCTCAGAGTTTCATTTTGATATCCACTACAACAAAAGATCAGAATTGAACTCTGTTGCCCCCTTTGAAATACATGGAATGGCAATCTGTTGGGAAGATTCTCCTGTGTACTATGTTAACATTCCTAAGGATCTGCTGTGGTCTGGCAGCAGCAGAAAAGACGAAGGGCTGCGTTTGACTGCATCAGGTGATAGAACTAATGTGTTACCTCTTGATGATATGTTGGAGCTGGCTAAACGTAGATGGAGCAGGATTGGTGAAATCATGGGGAAAAGAGGTGTCAGGAAATTTACTTGGATGTTGAAAAGTCAGATTCAGGCACTGAAGTGCCCTGCAGTTCAAATACAAAGATTTGGTTGCCAGAGTCTTGCAGGGAAAAGTATTTGTTTTGAAATTATAGACACCTCATTTTTATTGTTGCCACCAGTTCATATACAAGATGGCATTGACATGTGCATTGTGGCCTGGATTCTTTGGCCTGATGAGGAGAAAAAGTCTAATCCAAACTTGGAAAAGGAAGTTAAGAAAAGGTTATCCAGCGATGCTGCAGCAGCTGCAAATCGAAATGGCAGGTGGAAAAATCAGATGCGAAGAGCTGCACACAATGGTTGCTGCCGTCGAGTTGCTCAAACACGGGCCTTATGTTCTGTTCTGTGGAAGTTGCTTGTTTCTGAAGACCTTGCTGAGTCACTTATAAATATAGAAGTTCCATTGGTTAATATTCTTGCTGACATGGAGCTTTGGGGAGTGGGTCTTGATATGGAGGGATGTCTGCAAGCACGTAATGTGCTGGGGAAAAAGCTAAGACATCTTGAGAAAGAAGCTTATAAACTGGCTGGCATGACATTTTCATTATATACAGCAGCAGATATTGCTAATGTGCTTTATGGACACTTAAAGCTGCCCATACCAGAGGTGCGCAACAAGGGAAAACAACATCCAAGTACTGACAAGCATTGCTTGGATTTACTAAGGGATGAGCATCCTATTATTCCCATCATCAAAGAGCATCGTACATTGGCAAAACTCTTAAACTGTACGTTGGGATCAATTTGCTCACTTGCTAGGCTATCTGTGAAGACACAGAAGTACACATTGCATGGTCATTGGCTCCAAACATCAACAGCTACTGGTCGGCTTTCAATGGAGGAACCTAATCTCCAGTGTGTGGAACATATGGTTGACTTCAAAATGAACAAGGATGAAAATGGAGGCAAAAGTGATGTTGATCATTACAAAATTAATGCTCGAGAATACTTTATACCTACTCAGGACAACTGGATACTGTTGACCGCAGATTATTCACAGATAGAACTGCGGCTGATGGCCCATTTTTCTGAGGATTCCTCACTCATTGAACTCCTTAGTAAGCCCCATGGTGATGTCTTCACCATGATTGCAGCTAGATGGACCGGAATGTCAGAAGATTGTATTAGTTCTGATGTACGAGATCAGACCAAAAGGTTGGTGTATGGAATTCTCTACGGAATGGGTGCTAATAGCCTTGCAGAACAACTGGATTGCAGTCCCCAGGCAGCTTCTGAGAAAATACAAAACTTCAAAAGTTCTTTTCCCGGAGTTGCTTCTTGGCTCAATGAAGCAGTTGAATGTTGCCGCAAGAAAGGATATGTGGAAACCCTTAAGGGAAGAAAACGATTTTTGTCAAAAATAAAATTTGGGAACAGCAAGGAAAAGTCAAAGGCGCAGAGACAGGCTGTAAATTCGATATGTCAGGGATCTGCTGCTGACATAATTAAGATTGCAATGATAAATATTTACTCCTTAATAGTTGATGGAGCTGAAAAGCCTGATTCAACTTCTCCACTTGCAACAAAGTTTCACAAACTGAAGGGCCGCTGCCGAATTCTGTTGCAGGTACATGATGAATTGGTACTTGAAGTTGATCCTTCTGTGATAAGGGAAGCTGCTTTGCTGTTACAAACAAGCATGGAAAGTGCTGTTTCGCTTCTTGTTCCTATGCATGTCAAATTGAAAGTTGGAAGAACTTGGGGTTCTTTGCAACCCTTTCAGGCTGACCAATATGAAGATCTGCCTGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000724 GO:0000725 GO:0000726 GO:0002200 GO:0002376 GO:0002377 GO:0002440 GO:0002520 GO:0002566 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003684 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006282 GO:0006284 GO:0006302 GO:0006303 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009640 GO:0009653 GO:0009791 GO:0009888 GO:0009892 GO:0009933 GO:0009987 GO:0010468 GO:0010564 GO:0010569 GO:0010605 GO:0016043 GO:0016445 GO:0016446 GO:0016462 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016829 GO:0016835 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0031323 GO:0031324 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0042623 GO:0042802 GO:0043142 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045738 GO:0045910 GO:0045934 GO:0046483 GO:0048507 GO:0048519 GO:0048523 GO:0048532 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051239 GO:0051259 GO:0051260 GO:0051301 GO:0051575 GO:0051716 GO:0051726 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:0097681 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901987 GO:1902749 GO:1990067 GO:2000011 GO:2000026 GO:2000042 GO:2000779 GO:2000780 GO:2001020 GO:2001021
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD DNA_pol_A Helicase_C

Protein Analysis

2181

Amino Acids

240.33

Weight (kDa)

7.85

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 525 - 701 1.4e-18 DEAD/DEAH box helicase
Helicase_C PF00271 831 - 906 1.2e-09 Helicase conserved C-terminal domain
HTH_61 PF20470 911 - 1002 9.7e-25 Helix-turn-helix domain
POLQ_helical PF21099 1026 - 1210 1.5e-52 DNA_pol_Q helicase like region helical domain
DUF7898 PF25453 1216 - 1290 4e-07 Domain of unknown function (DUF7898)
DNA_pol_A PF00476 1752 - 2164 1.6e-110 DNA polymerase family A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013097)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32700
fragaria_vesca FvH4_2g36240 FvH4_2g36240
malus_domestica MD08G1120000.v1.1
prunus_persica Prupe.1G453900_v2.0.a1 Prupe.1G453900_v2.0.a1
pyrus_communis pycom15g09100
rosa_chinensis RchiOBHm_Chr6g0305641
rosa_laevigata RLG00000010870
rosa_multiflora Rmu_sc0001809.1_g000026
rosa_roxburghii Rroxscaffold_7G00162490
rosa_rugosa Rorug06G0342400 Rorug06G0342500
rosa_samantha Rh6AG454300 Rh6BG447200 Rh6CG468000 Rh6DG454800
rosa_wichuraiana Rw6G039610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 180, 2196, 5174, 5307
AarI CACCTGC 2 cut(s) 82, 5040
AasI GACNNNNNNGTC 1 cut(s) 5896
Acc16I TGCGCA 1 cut(s) 5399
Acc36I ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5040, 6343
Acc65I GGTACC 1 cut(s) 2155
AccB1I GGYRCC 2 cut(s) 2155, 4229
AccB7I CCANNNNNTGG 3 cut(s) 3968, 4220, 5647
AccI GTMKAC 1 cut(s) 4302
AccII CGCG 4 cut(s) 28, 2577, 2661, 3258
AccIII TCCGGA 1 cut(s) 3397
AcoI YGGCCR 1 cut(s) 1326
AcvI CACGTG 1 cut(s) 4045
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 6 cut(s) 131, 866, 992, 1388, 1712, 3299
AfiI CCNNNNNNNGG 8 cut(s) 1940, 2057, 2448, 2654, 3968, 4048, 4220, 5647
AflII CTTAAG 2 cut(s) 2372, 6119
AflIII ACRYGT 2 cut(s) 2575, 4929
AgeI ACCGGT 1 cut(s) 2152
AhlI ACTAGT 2 cut(s) 3457, 4022
AjiI CACGTC 1 cut(s) 3050
AjnI CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4943, 6009
AjuI GAANNNNNNNTTGG 4 cut(s) 3354, 3386, 3591, 3623
AleI CACNNNNGTG 1 cut(s) 4804
AloI GAACNNNNNNTCC 2 cut(s) 5622, 5654
Alw21I GWGCWC 3 cut(s) 2918, 3304, 3859
Alw26I GTCTC 3 cut(s) 538, 771, 6181
Alw44I GTGCAC 3 cut(s) 2914, 3300, 3855
AlwNI CAGNNNCTG 5 cut(s) 2111, 4801, 5033, 5609, 6020
Ama87I CYCGRG 2 cut(s) 3690, 5727
Aor13HI TCCGGA 1 cut(s) 3397
ApaLI GTGCAC 3 cut(s) 2914, 3300, 3855
ArsI GACNNNNNNTTYG 2 cut(s) 2683, 2715
AseI ATTAAT 1 cut(s) 5721
AsiGI ACCGGT 1 cut(s) 2152
Asp700I GAANNNNTTC 1 cut(s) 4453
Asp718I GGTACC 1 cut(s) 2155
AspLEI GCGC 4 cut(s) 30, 2811, 5400, 6184
AsuC2I CCSGG 3 cut(s) 2650, 4443, 6054
AsuII TTCGAA 3 cut(s) 50, 272, 1145
AvaI CYCGRG 2 cut(s) 3690, 5727
AvaII GGWCC 4 cut(s) 1267, 3517, 3934, 5889
AxyI CCTNAGG 1 cut(s) 4605
BaeGI GKGCMC 7 cut(s) 916, 2918, 3304, 3703, 3859, 4234, 4811
BalI TGGCCA 1 cut(s) 1328
BamHI GGATCC 1 cut(s) 2467
BanI GGYRCC 2 cut(s) 2155, 4229
BanII GRGCYC 1 cut(s) 2523
BarI GAAGNNNNNNTAC 2 cut(s) 2472, 2504
BauI CACGAG 1 cut(s) 1128
BbrPI CACGTG 1 cut(s) 4045
BbsI GAAGAC 3 cut(s) 5160, 5564, 5857
Bbv12I GWGCWC 3 cut(s) 2918, 3304, 3859
BceAI ACGGC 2 cut(s) 1863, 5079
BcgI CGANNNNNNTGC 2 cut(s) 3328, 3362
BciT130I CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4945, 6011
BciVI GTATCC 2 cut(s) 1105, 5754
BclI TGATCA 2 cut(s) 3403, 5707
BcnI CCSGG 3 cut(s) 2650, 4443, 6054
BcoDI GTCTC 3 cut(s) 538, 771, 6181
BcuI ACTAGT 2 cut(s) 3457, 4022
BfmI CTRYAG 6 cut(s) 114, 933, 1818, 4206, 4812, 5025
BfrI CTTAAG 2 cut(s) 2372, 6119
BfuAI ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5040, 6343
BfuI GTATCC 2 cut(s) 1105, 5754
BglII AGATCT 1 cut(s) 6526
BlpI GCTNAGC 1 cut(s) 135
BmcAI AGTACT 3 cut(s) 1438, 3388, 5425
Bme18I GGWCC 4 cut(s) 1267, 3517, 3934, 5889
BmeT110I CYCGRG 2 cut(s) 3690, 5727
BmgBI CACGTC 1 cut(s) 3050
BmiI GGNNCC 8 cut(s) 1784, 2157, 2469, 3936, 4083, 4231, 5591, 5631
BpiI GAAGAC 3 cut(s) 5160, 5564, 5857
BplI GAGNNNNNCTC 2 cut(s) 5810, 5842
BpmI CTGGAG 5 cut(s) 357, 414, 1754, 2517, 5624
Bpu10I CCTNAGC 4 cut(s) 360, 2599, 4059, 4309
Bpu1102I GCTNAGC 1 cut(s) 135
Bpu14I TTCGAA 3 cut(s) 50, 272, 1145
BpuEI CTTGAG 2 cut(s) 740, 5312
BpuMI CCSGG 3 cut(s) 2650, 4443, 6054
BsaAI YACGTR 2 cut(s) 4045, 5261
BsaBI GATNNNNATC 5 cut(s) 2259, 2341, 3147, 5460, 5706
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 3 cut(s) 2152, 3397, 5891
BsaXI ACNNNNNCTCC 6 cut(s) 2724, 2754, 4958, 4988, 5622, 5652
Bsc4I CCNNNNNNNGG 8 cut(s) 1940, 2057, 2448, 2654, 3968, 4048, 4220, 5647
Bse118I RCCGGY 1 cut(s) 2152
Bse21I CCTNAGG 1 cut(s) 4605
Bse3DI GCAATG 8 cut(s) 598, 672, 2287, 2804, 4408, 5434, 5573, 6249
Bse8I GATNNNNATC 5 cut(s) 2259, 2341, 3147, 5460, 5706
BseAI TCCGGA 1 cut(s) 3397
BseBI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4945, 6011
BseJI GATNNNNATC 5 cut(s) 2259, 2341, 3147, 5460, 5706
BseLI CCNNNNNNNGG 8 cut(s) 1940, 2057, 2448, 2654, 3968, 4048, 4220, 5647
BseMI GCAATG 8 cut(s) 598, 672, 2287, 2804, 4408, 5434, 5573, 6249
BseMII CTCAG 9 cut(s) 35, 429, 639, 1298, 4497, 5154, 5762, 5808, 6012
BseRI GAGGAG 3 cut(s) 9, 587, 4979
BseSI GKGCMC 7 cut(s) 916, 2918, 3304, 3703, 3859, 4234, 4811
BseYI CCCAGC 2 cut(s) 2516, 5269
BsgI GTGCAG 7 cut(s) 296, 1638, 1734, 1813, 1901, 3072, 5061
Bsh1236I CGCG 4 cut(s) 28, 2577, 2661, 3258
Bsh1285I CGRYCG 1 cut(s) 2153
BshNI GGYRCC 2 cut(s) 2155, 4229
BshTI ACCGGT 1 cut(s) 2152
BsiEI CGRYCG 1 cut(s) 2153
BsiHKAI GWGCWC 3 cut(s) 2918, 3304, 3859
BsiHKCI CYCGRG 2 cut(s) 3690, 5727
BsiSI CCGG 7 cut(s) 170, 2153, 2650, 3398, 4443, 5892, 6054
BsiWI CGTACG 1 cut(s) 3722
BslFI GGGAC 6 cut(s) 2689, 3388, 3920, 3963, 4374, 5991
BslI CCNNNNNNNGG 8 cut(s) 1940, 2057, 2448, 2654, 3968, 4048, 4220, 5647
BsmAI GTCTC 3 cut(s) 538, 771, 6181
BsmFI GGGAC 6 cut(s) 2689, 3388, 3920, 3963, 4374, 5991
BsmI GAATGC 1 cut(s) 3541
BsoBI CYCGRG 2 cut(s) 3690, 5727
Bsp119I TTCGAA 3 cut(s) 50, 272, 1145
Bsp1286I GDGCHC 8 cut(s) 916, 2523, 2918, 3304, 3703, 3859, 4234, 4811
Bsp13I TCCGGA 1 cut(s) 3397
Bsp1407I TGTACA 1 cut(s) 2290
Bsp1720I GCTNAGC 1 cut(s) 135
Bsp19I CCATGG 1 cut(s) 5854
Bsp68I TCGCGA 1 cut(s) 2661
BspCNI CTCAG 9 cut(s) 34, 428, 640, 1297, 4496, 5155, 5761, 5809, 6013
BspEI TCCGGA 1 cut(s) 3397
BspFNI CGCG 4 cut(s) 28, 2577, 2661, 3258
BspLI GGNNCC 8 cut(s) 1784, 2157, 2469, 3936, 4083, 4231, 5591, 5631
BspMAI CTGCAG 2 cut(s) 4816, 5029
BspMI ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5040, 6343
BspQI GCTCTTC 2 cut(s) 647, 5065
BspT104I TTCGAA 3 cut(s) 50, 272, 1145
BspT107I GGYRCC 2 cut(s) 2155, 4229
BspTI CTTAAG 2 cut(s) 2372, 6119
BsrDI GCAATG 8 cut(s) 598, 672, 2287, 2804, 4408, 5434, 5573, 6249
BsrFI RCCGGY 1 cut(s) 2152
BsrGI TGTACA 1 cut(s) 2290
BssAI RCCGGY 1 cut(s) 2152
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1128
BssT1I CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5854
Bst2BI CACGAG 1 cut(s) 1128
Bst2UI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4945, 6011
Bst6I CTCTTC 6 cut(s) 307, 647, 1866, 2754, 3879, 5065
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 2372, 6119
BstAPI GCANNNNNTGC 4 cut(s) 2108, 3299, 5033, 5351
BstAUI TGTACA 1 cut(s) 2290
BstBAI YACGTR 2 cut(s) 4045, 5261
BstBI TTCGAA 3 cut(s) 50, 272, 1145
BstDSI CCRYGG 1 cut(s) 5854
BstEII GGTNACC 1 cut(s) 697
BstENI CCTNNNNNAGG 1 cut(s) 2446
BstFNI CGCG 4 cut(s) 28, 2577, 2661, 3258
BstHHI GCGC 4 cut(s) 30, 2811, 5400, 6184
BstMAI GTCTC 3 cut(s) 538, 771, 6181
BstMCI CGRYCG 1 cut(s) 2153
BstNI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4945, 6011
BstNSI RCATGY 4 cut(s) 747, 2360, 4933, 6464
BstPI GGTNACC 1 cut(s) 697
BstSFI CTRYAG 6 cut(s) 114, 933, 1818, 4206, 4812, 5025
BstSLI GKGCMC 7 cut(s) 916, 2918, 3304, 3703, 3859, 4234, 4811
BstUI CGCG 4 cut(s) 28, 2577, 2661, 3258
BstV2I GAAGAC 3 cut(s) 5160, 5564, 5857
BstX2I RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4345, 4609, 6215, 6526
BstXI CCANNNNNNTGG 4 cut(s) 1793, 2516, 3248, 5944
BstYI RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4345, 4609, 6215, 6526
Bsu36I CCTNAGG 1 cut(s) 4605
BsuI GTATCC 2 cut(s) 1105, 5754
BtgI CCRYGG 1 cut(s) 5854
BtgZI GCGATG 3 cut(s) 150, 2885, 5034
BtrI CACGTC 1 cut(s) 3050
BtsI GCAGTG 3 cut(s) 493, 1100, 1191
BtsIMutI CAGTG 9 cut(s) 493, 988, 1100, 1191, 1457, 2397, 2413, 4799, 5648
BtuMI TCGCGA 1 cut(s) 2661
BveI ACCTGC 7 cut(s) 82, 1540, 1610, 1648, 2561, 5040, 6343
CaiI CAGNNNCTG 5 cut(s) 2111, 4801, 5033, 5609, 6020
CfoI GCGC 4 cut(s) 30, 2811, 5400, 6184
Cfr10I RCCGGY 1 cut(s) 2152
CseI GACGC 3 cut(s) 2566, 3825, 4371
CsiI ACCWGGT 2 cut(s) 862, 1384
CspAI ACCGGT 1 cut(s) 2152
CspCI CAANNNNNGTGG 2 cut(s) 3025, 3060
DraI TTTAAA 4 cut(s) 589, 2925, 3499, 3916
DraIII CACNNNGTG 6 cut(s) 131, 866, 992, 1388, 1712, 3299
DrdI GACNNNNNNGTC 1 cut(s) 5896
DseDI GACNNNNNNGTC 1 cut(s) 5896
EaeI YGGCCR 1 cut(s) 1326
Eam1104I CTCTTC 6 cut(s) 307, 647, 1866, 2754, 3879, 5065
EarI CTCTTC 6 cut(s) 307, 647, 1866, 2754, 3879, 5065
EciI GGCGGA 1 cut(s) 3896
Eco130I CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5854
Eco24I GRGCYC 1 cut(s) 2523
Eco32I GATATC 2 cut(s) 4329, 4501
Eco47I GGWCC 4 cut(s) 1267, 3517, 3934, 5889
Eco72I CACGTG 1 cut(s) 4045
Eco81I CCTNAGG 1 cut(s) 4605
Eco88I CYCGRG 2 cut(s) 3690, 5727
Eco91I GGTNACC 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 2446
EcoO109I RGGNCCY 2 cut(s) 3934, 4418
EcoO65I GGTNACC 1 cut(s) 697
EcoRI GAATTC 2 cut(s) 5955, 6342
EcoRII CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4943, 6009
EcoRV GATATC 2 cut(s) 4329, 4501
EcoT14I CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5854
EcoT22I ATGCAT 1 cut(s) 6462
EcoT38I GRGCYC 1 cut(s) 2523
ErhI CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5854
FaqI GGGAC 6 cut(s) 2689, 3388, 3920, 3963, 4374, 5991
FauI CCCGC 1 cut(s) 370
FauNDI CATATG 1 cut(s) 5655
FbaI TGATCA 2 cut(s) 3403, 5707
FblI GTMKAC 1 cut(s) 4302
FriOI GRGCYC 1 cut(s) 2523
FspI TGCGCA 1 cut(s) 5399
GlaI GCGC 4 cut(s) 29, 2810, 5399, 6183
GsaI CCCAGC 2 cut(s) 2520, 5273
GsuI CTGGAG 5 cut(s) 357, 414, 1754, 2517, 5624
HapII CCGG 7 cut(s) 170, 2153, 2650, 3398, 4443, 5892, 6054
HgaI GACGC 3 cut(s) 2566, 3825, 4371
HhaI GCGC 4 cut(s) 30, 2811, 5400, 6184
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 4 cut(s) 28, 2809, 5398, 6182
HinP1I GCGC 4 cut(s) 28, 2809, 5398, 6182
HincII GTYRAC 7 cut(s) 1965, 2239, 2389, 4116, 4597, 5662, 5769
HindII GTYRAC 7 cut(s) 1965, 2239, 2389, 4116, 4597, 5662, 5769
HindIII AAGCTT 4 cut(s) 870, 1535, 3492, 5301
HpaI GTTAAC 1 cut(s) 4597
HpaII CCGG 7 cut(s) 170, 2153, 2650, 3398, 4443, 5892, 6054
Hpy99I CGWCG 1 cut(s) 5100
HpyCH4IV ACGT 8 cut(s) 105, 628, 774, 3049, 4044, 4717, 5260, 5519
HpySE526I ACGT 8 cut(s) 105, 628, 774, 3049, 4044, 4717, 5260, 5519
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 4 cut(s) 28, 2809, 5398, 6182
Kpn2I TCCGGA 1 cut(s) 3397
KpnI GGTACC 1 cut(s) 2159
Ksp22I TGATCA 2 cut(s) 3403, 5707
KspAI GTTAAC 1 cut(s) 4597
LguI GCTCTTC 2 cut(s) 647, 5065
LmnI GCTCC 8 cut(s) 1735, 3992, 4060, 4705, 4725, 5215, 5595, 6275
MabI ACCWGGT 2 cut(s) 862, 1384
MaeII ACGT 8 cut(s) 105, 628, 774, 3049, 4044, 4717, 5260, 5519
MaeIII GTNAC 9 cut(s) 697, 1315, 2422, 3163, 3265, 3559, 4683, 5166, 6414
MfeI CAATTG 2 cut(s) 636, 4215
MflI RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4345, 4609, 6215, 6526
MhlI GDGCHC 8 cut(s) 916, 2523, 2918, 3304, 3703, 3859, 4234, 4811
MlsI TGGCCA 1 cut(s) 1328
MluI ACGCGT 1 cut(s) 2575
MluNI TGGCCA 1 cut(s) 1328
MlyI GAGTC 5 cut(s) 2640, 2836, 4346, 4852, 5174
MmeI TCCRAC 4 cut(s) 3589, 3986, 4683, 6457
Mox20I TGGCCA 1 cut(s) 1328
Mph1103I ATGCAT 1 cut(s) 6462
MroI TCCGGA 1 cut(s) 3397
MroXI GAANNNNTTC 1 cut(s) 4453
MscI TGGCCA 1 cut(s) 1328
MslI CAYNNNNRTG 9 cut(s) 318, 324, 993, 1203, 2177, 2904, 4804, 4928, 5859
Msp20I TGGCCA 1 cut(s) 1328
MspA1I CMGCKG 8 cut(s) 218, 392, 1985, 2037, 2606, 2979, 5033, 6336
MspCI CTTAAG 2 cut(s) 2372, 6119
MspI CCGG 7 cut(s) 170, 2153, 2650, 3398, 4443, 5892, 6054
MunI CAATTG 2 cut(s) 636, 4215
Mva1269I GAATGC 1 cut(s) 3541
MvaI CCWGG 7 cut(s) 795, 864, 917, 1386, 3521, 4945, 6011
MvnI CGCG 4 cut(s) 28, 2577, 2661, 3258
NciI CCSGG 3 cut(s) 2650, 4443, 6054
NcoI CCATGG 1 cut(s) 5854
NdeI CATATG 1 cut(s) 5655
NlaIV GGNNCC 8 cut(s) 1784, 2157, 2469, 3936, 4083, 4231, 5591, 5631
NmuCI GTSAC 3 cut(s) 2422, 3265, 5166
NruI TCGCGA 1 cut(s) 2661
NsbI TGCGCA 1 cut(s) 5399
NsiI ATGCAT 1 cut(s) 6462
NspI RCATGY 4 cut(s) 747, 2360, 4933, 6464
NspV TTCGAA 3 cut(s) 50, 272, 1145
OliI CACNNNNGTG 1 cut(s) 4804
PaeR7I CTCGAG 2 cut(s) 3690, 5727
PaqCI CACCTGC 2 cut(s) 82, 5040
PciI ACATGT 1 cut(s) 4929
PciSI GCTCTTC 2 cut(s) 647, 5065
PcsI WCGNNNNNNNCGW 1 cut(s) 2707
PctI GAATGC 1 cut(s) 3541
PdmI GAANNNNTTC 1 cut(s) 4453
Pfl23II CGTACG 1 cut(s) 3722
PflMI CCANNNNNTGG 3 cut(s) 3968, 4220, 5647
PfoI TCCNGGA 1 cut(s) 6052
PinAI ACCGGT 1 cut(s) 2152
PleI GAGTC 5 cut(s) 2639, 2836, 4345, 4851, 5173
PmaCI CACGTG 1 cut(s) 4045
PmlI CACGTG 1 cut(s) 4045
PpsI GAGTC 5 cut(s) 2639, 2836, 4345, 4851, 5173
Ppu21I YACGTR 2 cut(s) 4045, 5261
PpuMI RGGWCCY 1 cut(s) 3934
PscI ACATGT 1 cut(s) 4929
PshBI ATTAAT 1 cut(s) 5721
PsiI TTATAA 4 cut(s) 180, 2196, 5174, 5307
Psp5II RGGWCCY 1 cut(s) 3934
Psp6I CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4943, 6009
PspCI CACGTG 1 cut(s) 4045
PspEI GGTNACC 1 cut(s) 697
PspFI CCCAGC 2 cut(s) 2516, 5269
PspGI CCWGG 7 cut(s) 793, 862, 915, 1384, 3519, 4943, 6009
PspLI CGTACG 1 cut(s) 3722
PspN4I GGNNCC 8 cut(s) 1784, 2157, 2469, 3936, 4083, 4231, 5591, 5631
PspPPI RGGWCCY 1 cut(s) 3934
PstI CTGCAG 2 cut(s) 4816, 5029
PstNI CAGNNNCTG 5 cut(s) 2111, 4801, 5033, 5609, 6020
PsuI RGATCY 8 cut(s) 1234, 1474, 2254, 2467, 4345, 4609, 6215, 6526
PvuII CAGCTG 4 cut(s) 392, 1985, 2606, 5033
RruI TCGCGA 1 cut(s) 2661
RseI CAYNNNNRTG 9 cut(s) 318, 324, 993, 1203, 2177, 2904, 4804, 4928, 5859
SapI GCTCTTC 2 cut(s) 647, 5065
ScaI AGTACT 3 cut(s) 1438, 3388, 5425
SchI GAGTC 5 cut(s) 2640, 2836, 4346, 4852, 5174
SduI GDGCHC 8 cut(s) 916, 2523, 2918, 3304, 3703, 3859, 4234, 4811
SexAI ACCWGGT 2 cut(s) 862, 1384
SfcI CTRYAG 6 cut(s) 114, 933, 1818, 4206, 4812, 5025
Sfr274I CTCGAG 2 cut(s) 3690, 5727
SfuI TTCGAA 3 cut(s) 50, 272, 1145
SinI GGWCC 4 cut(s) 1267, 3517, 3934, 5889
SlaI CTCGAG 2 cut(s) 3690, 5727
SmiMI CAYNNNNRTG 9 cut(s) 318, 324, 993, 1203, 2177, 2904, 4804, 4928, 5859
SmlI CTYRAG 6 cut(s) 719, 2372, 3690, 5291, 5727, 6119
SmoI CTYRAG 6 cut(s) 719, 2372, 3690, 5291, 5727, 6119
SpeI ACTAGT 2 cut(s) 3457, 4022
SspI AATATT 4 cut(s) 2316, 4468, 5200, 6253
StyI CCWWGG 6 cut(s) 405, 1544, 1786, 2242, 3043, 5854
TaiI ACGT 8 cut(s) 108, 631, 777, 3052, 4047, 4720, 5263, 5522
TatI WGTACW 8 cut(s) 1436, 1690, 2290, 2740, 3386, 4587, 5423, 5568
TauI GCSGC 5 cut(s) 221, 1657, 5800, 6096, 6336
TscAI CASTG 9 cut(s) 493, 995, 1107, 1198, 1464, 2404, 2413, 4806, 5648
TseFI GTSAC 3 cut(s) 2422, 3265, 5166
Tsp45I GTSAC 3 cut(s) 2422, 3265, 5166
TspGWI ACGGA 5 cut(s) 73, 1259, 2296, 3235, 5979
TspRI CASTG 9 cut(s) 493, 995, 1107, 1198, 1464, 2404, 2413, 4806, 5648
Van91I CCANNNNNTGG 3 cut(s) 3968, 4220, 5647
Vha464I CTTAAG 2 cut(s) 2372, 6119
VneI GTGCAC 3 cut(s) 2914, 3300, 3855
VpaK11BI GGWCC 4 cut(s) 1267, 3517, 3934, 5889
VspI ATTAAT 1 cut(s) 5721
XagI CCTNNNNNAGG 1 cut(s) 2446
XbaI TCTAGA 3 cut(s) 769, 1477, 3001
XceI RCATGY 4 cut(s) 747, 2360, 4933, 6464
XcmI CCANNNNNNNNNTGG 1 cut(s) 1336
XhoI CTCGAG 2 cut(s) 3690, 5727
XmiI GTMKAC 1 cut(s) 4302
XmnI GAANNNNTTC 1 cut(s) 4453
ZrmI AGTACT 3 cut(s) 1438, 3388, 5425
Zsp2I ATGCAT 1 cut(s) 6462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.