AT4G33410

signal peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014178
Physical Location & Seq
Forward (+)
16080760 .. 16083398
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G33410.1

Sequence Viewer

Length: 1119 bp
ATGGAGACTCTGTGGACTCTTTTGTATTTGCTAGAACCTGCTCCAGCTACTCTCATTGTCACAGCTGTCACCGTGACATTTGCATCCGCTTTCCGTGCACTTAACTACGGGAAAGAGATGGAGAGAAACCGTGACTTCTCTGAAGCATCCATAACACTAGATAGCTCTCAGGCCTTGATGATCCCAGTTATGAGCTCTTGCAGTTTGCTTCTCATGTTCTATTTGTTCTCTTCTGTTTCTCAGCTCCTGACCGCGTTTACAGCCATCGCCTCTGTTTCTTCTCTCTTCTATTGGTTATCACCATATGCGGTATATATGAAGACGCAGCTTGGTCTATCTGATCCCTTTCTTTCACGCTGCTGCTCCAAATCGTTTACAAGAATCCAAGGATTGCTACTTGTGGCTTGTGCTATGACAGTTGTGGCATGGCTCATCTCTGGCCATTGGGTGTTGAACAATTTGCTCGGAATCTCCATTTGTATTGCGTTTGTTAGCCATGTTCGTCTTCCTAATATCAAAATCTGTGCAATGCTCCTCGTGTGTCTCTTTGTATATGACATATTCTGGGTTTTCTTCTCTGAGAGATTCTTTGGTGCTAACGTTATGGTGGCCGTGGCAACCCAGCAAGCATCAAACCCAGTTCACACAGTGGCTAACAGTTTGAATCTTCCTGGACTGCAATTGATCACAAAGAAACTGGAACTACCTGTTAAAATTGTGTTTCCTCGGAACCTATTGGGCGGTGTGGTGCCCGGTGTGAGTGCCTCAGATTTCATGATGCTTGGTCTTGGTGACATGGCTATCCCTGCAATGCTTTTGGCTTTGGTCCTCTGCTTTGACCATCGAAAAACTAGAGATGTGGTGAATATTTTTGATCTAAAATCTTCCAAGGGACACAAATACATTTGGTATGCACTTCCCGGATATGCCATTGGCTTGGTGGCGGCTCTAGCTGCAGGTGTCTTGACCCACTCACCTCAGCCAGCTCTACTTTATCTGGTACCATCTACGTTAGGACCGGTGATCTTCATGTCATGGCGGAGAAAGGATCTTGCGGAGCTGTGGGAAGGACCTGCATTATCTAATCCCATTGAGAAATCTCATGAAATAGAGATCTGA

Protein Analysis

372

Amino Acids

41.0

Weight (kDa)

8.37

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_A22B PF04258 49 - 357 1.4e-70 Signal peptide peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33410
fragaria_vesca FvH4_7g29650
malus_domestica MD01G1203500.v1.1 MD07G1272800.v1.1
prunus_persica Prupe.2G296100_v2.0.a1 Prupe.2G296100_v2.0.a1
pyrus_communis pycom01g21260 pycom07g24920
rosa_chinensis RchiOBHm_Chr1g0377661
rosa_laevigata RLG00000026507
rosa_rugosa Rorug01G0404000 Rorug01G0404100
rosa_samantha Rh1AG428400 Rh1BG380900 Rh1CG394900 Rh1DG411900
rosa_wichuraiana Rw1G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 947
Acc36I ACCTGC 3 cut(s) 46, 947, 1081
Acc65I GGTACC 1 cut(s) 1000
AccB1I GGYRCC 2 cut(s) 748, 1000
AccII CGCG 1 cut(s) 254
AciI CCGC 7 cut(s) 87, 252, 308, 741, 944, 1039, 1055
AclI AACGTT 1 cut(s) 600
AclWI GGATC 3 cut(s) 175, 335, 1056
AcoI YGGCCR 2 cut(s) 439, 609
AcuI CTGAAG 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 1 cut(s) 1002
AgeI ACCGGT 1 cut(s) 1018
AgsI TTSAA 2 cut(s) 454, 664
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 9 cut(s) 47, 65, 165, 195, 244, 328, 953, 986, 1060
AluI AGCT 9 cut(s) 47, 65, 165, 195, 244, 328, 953, 986, 1060
Alw21I GWGCWC 2 cut(s) 100, 197
Alw26I GTCTC 1 cut(s) 548
Alw44I GTGCAC 1 cut(s) 96
AlwI GGATC 3 cut(s) 175, 335, 1056
AlwNI CAGNNNCTG 1 cut(s) 247
AoxI GGCC 3 cut(s) 171, 439, 609
ApaLI GTGCAC 1 cut(s) 96
ApeKI GCWGC 4 cut(s) 325, 357, 360, 953
AsiGI ACCGGT 1 cut(s) 1018
Asp718I GGTACC 1 cut(s) 1000
AspS9I GGNCC 3 cut(s) 826, 1016, 1070
AsuC2I CCSGG 2 cut(s) 753, 921
AsuHPI GGTGA 6 cut(s) 61, 291, 803, 874, 966, 1033
AvaII GGWCC 3 cut(s) 826, 1016, 1070
BaeGI GKGCMC 2 cut(s) 100, 753
BalI TGGCCA 1 cut(s) 441
BanI GGYRCC 2 cut(s) 748, 1000
BanII GRGCYC 1 cut(s) 197
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 2 cut(s) 326, 497
Bbv12I GWGCWC 2 cut(s) 100, 197
BbvCI CCTCAGC 1 cut(s) 978
BbvI GCAGC 4 cut(s) 337, 344, 347, 940
BccI CCATC 4 cut(s) 112, 272, 849, 1012
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 1 cut(s) 672
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 2 cut(s) 753, 921
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 4 cut(s) 32, 158, 852, 950
BfmI CTRYAG 1 cut(s) 954
BfuAI ACCTGC 3 cut(s) 46, 947, 1081
BglII AGATCT 1 cut(s) 1113
BisI GCNGC 5 cut(s) 326, 358, 361, 945, 954
BlsI GCNGC 5 cut(s) 327, 359, 362, 946, 955
Bme1390I CCNGG 3 cut(s) 672, 753, 921
Bme18I GGWCC 3 cut(s) 826, 1016, 1070
BmgT120I GGNCC 3 cut(s) 826, 1016, 1070
BmiI GGNNCC 3 cut(s) 731, 750, 1002
BmrFI CCNGG 3 cut(s) 672, 753, 921
BmrI ACTGGG 2 cut(s) 179, 632
BmsI GCATC 4 cut(s) 92, 155, 638, 768
BmuI ACTGGG 2 cut(s) 179, 632
BpiI GAAGAC 2 cut(s) 326, 497
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 978
BpuMI CCSGG 2 cut(s) 753, 921
BsaJI CCNNGG 4 cut(s) 385, 612, 725, 888
BsaWI WCCGGW 1 cut(s) 1018
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 3 cut(s) 185, 638, 702
Bse3DI GCAATG 2 cut(s) 534, 816
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 4 cut(s) 385, 612, 725, 888
BseGI GGATG 2 cut(s) 83, 146
BseMI GCAATG 2 cut(s) 534, 816
BseMII CTCAG 5 cut(s) 182, 254, 570, 780, 992
BseNI ACTGG 3 cut(s) 185, 638, 702
BseRI GAGGAG 1 cut(s) 524
BseSI GKGCMC 2 cut(s) 100, 753
BseXI GCAGC 4 cut(s) 337, 344, 347, 940
BseYI CCCAGC 1 cut(s) 621
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 3 cut(s) 173, 441, 611
BshNI GGYRCC 2 cut(s) 748, 1000
BshTI ACCGGT 1 cut(s) 1018
BsiHKAI GWGCWC 2 cut(s) 100, 197
BsiSI CCGG 3 cut(s) 753, 921, 1019
BslFI GGGAC 1 cut(s) 906
BsmAI GTCTC 1 cut(s) 548
BsmFI GGGAC 1 cut(s) 906
BsnI GGCC 3 cut(s) 173, 441, 611
Bsp1286I GDGCHC 3 cut(s) 100, 197, 753
Bsp143I GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
BspACI CCGC 7 cut(s) 87, 252, 308, 741, 944, 1039, 1055
BspANI GGCC 3 cut(s) 173, 441, 611
BspCNI CTCAG 5 cut(s) 181, 253, 571, 779, 991
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 2 cut(s) 774, 1102
BspLI GGNNCC 3 cut(s) 731, 750, 1002
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 3 cut(s) 46, 947, 1081
BspPI GGATC 3 cut(s) 175, 335, 1056
BspT107I GGYRCC 2 cut(s) 748, 1000
BsrDI GCAATG 2 cut(s) 534, 816
BsrFI RCCGGY 1 cut(s) 1018
BsrI ACTGG 3 cut(s) 185, 638, 702
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 4 cut(s) 385, 612, 725, 888
BssMI GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 2 cut(s) 385, 888
Bst2BI CACGAG 1 cut(s) 536
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 5 cut(s) 73, 131, 418, 649, 659
Bst6I CTCTTC 2 cut(s) 235, 290
BstC8I GCNNGC 2 cut(s) 627, 984
BstDEI CTNAG 5 cut(s) 168, 240, 579, 766, 978
BstDSI CCRYGG 1 cut(s) 612
BstF5I GGATG 2 cut(s) 83, 146
BstFNI CGCG 1 cut(s) 254
BstKTI GATC 7 cut(s) 183, 343, 687, 877, 1026, 1051, 1116
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
BstMWI GCNNNNNNNGC 5 cut(s) 95, 260, 806, 950, 953
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 3 cut(s) 670, 751, 919
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 2 cut(s) 100, 753
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 4 cut(s) 337, 344, 347, 940
BstV2I GAAGAC 2 cut(s) 326, 497
BstX2I RGATCY 2 cut(s) 1048, 1113
BstXI CCANNNNNNTGG 1 cut(s) 937
BstYI RGATCY 2 cut(s) 1048, 1113
BsuRI GGCC 3 cut(s) 173, 441, 611
BtgI CCRYGG 1 cut(s) 612
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 2 cut(s) 83, 146
BtsIMutI CAGTG 1 cut(s) 654
BveI ACCTGC 3 cut(s) 46, 947, 1081
Cac8I GCNNGC 2 cut(s) 627, 984
CaiI CAGNNNCTG 1 cut(s) 247
CciI TCATGA 2 cut(s) 774, 1102
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 3 cut(s) 826, 1016, 1070
CseI GACGC 1 cut(s) 331
Csp6I GTAC 1 cut(s) 1001
CspAI ACCGGT 1 cut(s) 1018
CviAII CATG 8 cut(s) 214, 426, 497, 775, 796, 1030, 1035, 1103
CviQI GTAC 1 cut(s) 1001
DdeI CTNAG 5 cut(s) 168, 240, 579, 766, 978
DpnI GATC 7 cut(s) 182, 342, 686, 876, 1025, 1050, 1115
DpnII GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
DraIII CACNNNGTG 1 cut(s) 649
EaeI YGGCCR 2 cut(s) 439, 609
Eam1104I CTCTTC 2 cut(s) 235, 290
EarI CTCTTC 2 cut(s) 235, 290
EciI GGCGGA 1 cut(s) 1054
Ecl136II GAGCTC 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 385, 888
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 197
Eco47I GGWCC 3 cut(s) 826, 1016, 1070
Eco53kI GAGCTC 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 1070
EcoRII CCWGG 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 385, 888
EcoT38I GRGCYC 1 cut(s) 197
ErhI CCWWGG 2 cut(s) 385, 888
FaeI CATG 8 cut(s) 217, 429, 500, 778, 799, 1033, 1038, 1106
FaqI GGGAC 1 cut(s) 906
FatI CATG 8 cut(s) 213, 425, 496, 774, 795, 1029, 1034, 1102
FauNDI CATATG 1 cut(s) 304
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 5 cut(s) 326, 358, 361, 945, 954
FokI GGATG 2 cut(s) 70, 133
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 5 cut(s) 326, 358, 361, 945, 954
FspBI CTAG 4 cut(s) 32, 158, 852, 950
GluI GCNGC 5 cut(s) 326, 358, 361, 945, 954
GsaI CCCAGC 1 cut(s) 625
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 3 cut(s) 173, 441, 611
HapII CCGG 3 cut(s) 753, 921, 1019
HgaI GACGC 1 cut(s) 331
Hin1II CATG 8 cut(s) 217, 429, 500, 778, 799, 1033, 1038, 1106
HinfI GANTC 6 cut(s) 7, 16, 381, 468, 585, 664
HpaII CCGG 3 cut(s) 753, 921, 1019
HphI GGTGA 6 cut(s) 61, 291, 803, 874, 966, 1033
Hpy166II GTNNAC 5 cut(s) 15, 98, 258, 375, 643
Hpy188I TCNGA 7 cut(s) 142, 340, 467, 580, 729, 769, 1118
Hpy188III TCNNGA 4 cut(s) 247, 775, 964, 1103
Hpy8I GTNNAC 5 cut(s) 15, 98, 258, 375, 643
HpyAV CCTTC 1 cut(s) 1061
HpyCH4III ACNGT 5 cut(s) 73, 131, 418, 649, 659
HpyCH4IV ACGT 2 cut(s) 600, 1010
HpyCH4V TGCA 9 cut(s) 83, 98, 201, 527, 679, 809, 914, 956, 1076
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 260, 806, 950, 953
HpyF3I CTNAG 5 cut(s) 168, 240, 579, 766, 978
HpySE526I ACGT 2 cut(s) 600, 1010
Hsp92II CATG 8 cut(s) 217, 429, 500, 778, 799, 1033, 1038, 1106
KpnI GGTACC 1 cut(s) 1004
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
LmnI GCTCC 5 cut(s) 46, 249, 368, 537, 1057
Lsp1109I GCAGC 4 cut(s) 337, 344, 347, 940
LweI GCATC 4 cut(s) 92, 155, 638, 768
MaeI CTAG 4 cut(s) 32, 158, 852, 950
MaeII ACGT 2 cut(s) 600, 1010
MaeIII GTNAC 5 cut(s) 58, 67, 73, 131, 791
MalI GATC 7 cut(s) 182, 342, 686, 876, 1025, 1050, 1115
MboI GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
MboII GAAGA 9 cut(s) 222, 270, 277, 331, 497, 565, 659, 876, 1018
MfeI CAATTG 1 cut(s) 680
MflI RGATCY 2 cut(s) 1048, 1113
MhlI GDGCHC 3 cut(s) 100, 197, 753
MlsI TGGCCA 1 cut(s) 441
MluCI AATT 3 cut(s) 457, 680, 714
MluNI TGGCCA 1 cut(s) 441
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 6 cut(s) 280, 545, 735, 775, 839, 987
Mox20I TGGCCA 1 cut(s) 441
MscI TGGCCA 1 cut(s) 441
MseI TTAA 2 cut(s) 102, 711
Msp20I TGGCCA 1 cut(s) 441
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 3 cut(s) 753, 921, 1019
MspR9I CCNGG 3 cut(s) 672, 753, 921
MunI CAATTG 1 cut(s) 680
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 5 cut(s) 95, 260, 806, 950, 953
NciI CCSGG 2 cut(s) 753, 921
NdeI CATATG 1 cut(s) 304
NdeII GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
NlaIII CATG 8 cut(s) 217, 429, 500, 778, 799, 1033, 1038, 1106
NlaIV GGNNCC 3 cut(s) 731, 750, 1002
NmuCI GTSAC 5 cut(s) 58, 67, 73, 131, 791
PagI TCATGA 2 cut(s) 774, 1102
PaqCI CACCTGC 1 cut(s) 947
PceI AGGCCT 1 cut(s) 173
PfeI GAWTC 4 cut(s) 381, 468, 585, 664
PfoI TCCNGGA 2 cut(s) 670, 919
PinAI ACCGGT 1 cut(s) 1018
PkrI GCNGC 5 cut(s) 327, 359, 362, 946, 955
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 1070
Psp124BI GAGCTC 1 cut(s) 197
Psp1406I AACGTT 1 cut(s) 600
Psp5II RGGWCCY 1 cut(s) 1070
Psp6I CCWGG 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 621
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 731, 750, 1002
PspPI GGNCC 3 cut(s) 826, 1016, 1070
PspPPI RGGWCCY 1 cut(s) 1070
PstI CTGCAG 1 cut(s) 958
PstNI CAGNNNCTG 1 cut(s) 247
PsuI RGATCY 2 cut(s) 1048, 1113
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 1002
RsaNI GTAC 1 cut(s) 1001
SacI GAGCTC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 102, 711
SatI GCNGC 5 cut(s) 326, 358, 361, 945, 954
Sau3AI GATC 7 cut(s) 180, 340, 684, 874, 1023, 1048, 1113
Sau96I GGNCC 3 cut(s) 826, 1016, 1070
SchI GAGTC 1 cut(s) 10
ScrFI CCNGG 3 cut(s) 672, 753, 921
SduI GDGCHC 3 cut(s) 100, 197, 753
SfaNI GCATC 4 cut(s) 92, 155, 638, 768
SfcI CTRYAG 1 cut(s) 954
SinI GGWCC 3 cut(s) 826, 1016, 1070
Sse9I AATT 3 cut(s) 457, 680, 714
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 7 cut(s) 87, 252, 308, 741, 944, 1039, 1055
SspI AATATT 1 cut(s) 868
SspMI CTAG 4 cut(s) 32, 158, 852, 950
SstI GAGCTC 1 cut(s) 197
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 3 cut(s) 670, 751, 919
StyI CCWWGG 2 cut(s) 385, 888
TaaI ACNGT 5 cut(s) 73, 131, 418, 649, 659
TaiI ACGT 2 cut(s) 603, 1013
TaqI TCGA 1 cut(s) 844
TasI AATT 3 cut(s) 457, 680, 714
TauI GCSGC 1 cut(s) 947
TfiI GAWTC 4 cut(s) 381, 468, 585, 664
Tru1I TTAA 2 cut(s) 102, 711
Tru9I TTAA 2 cut(s) 102, 711
TscAI CASTG 1 cut(s) 654
TseFI GTSAC 5 cut(s) 58, 67, 73, 131, 791
TseI GCWGC 4 cut(s) 325, 357, 360, 953
Tsp45I GTSAC 5 cut(s) 58, 67, 73, 131, 791
TspDTI ATGAA 4 cut(s) 332, 763, 1018, 1119
TspGWI ACGGA 1 cut(s) 83
TspRI CASTG 1 cut(s) 654
VneI GTGCAC 1 cut(s) 96
VpaK11BI GGWCC 3 cut(s) 826, 1016, 1070
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XspI CTAG 4 cut(s) 32, 158, 852, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.