Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014178

Physical Location & Seq

Genomic Coordinates Forward (+)

29930972 .. 29933782

Gene Structure 2811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1203500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1113 bp

ATGGAGCCTCTCTGGAAGCTTTTTTATTTGCTGGAGCCTGCACCTTTTACTCTAGTTGTAACGGCAGTAGGCGTGACTTTTGGATCTGCATTTCGGGCTCTAAATTATGGGAAAGAAATGGAGAAAAACCATGATTTATCCGAAACATCAATTACGTTAGATAGGTCCCAAGCACTCATGATCCCGGTGATGAGCTCTGTAAGCTTGCTTTTGATGTTCTACTTGTTCACTTCCGTCTCACAACTCCTCACTGTATTCACAGCCGTTGCCTCCGTTTCCTCCCTGTTTTTCTTCCTATCTCCTTATGCAGCCTATTTGAAGTCACAATTTGGTCTTGCGGATCCATATGTGTCAAGGTGTTGTTCCAAGTCATTTACGCGAATCCAAGCCCTGTTATTGCTGTTATGCATTGGTACAGTTGCTGCTTGGCTTGTTTCTGGGCACTGGGTATTGAACAATTTGTTGGGCATTTCCATATGTATTGCCTTTGTGAGTCATGTTCGTCTTCCAAACATCAAAATTTGTGCAATGCTCCTTGTCTGCCTATTTGTATATGACATATTCTGGGTTTTCTTCTCTGAGAGATTTTTTGGGGCAAATGTCATGGTATCAGTGGCAACACAACAAGCCTCAAACCCAGTTCATACTGTTGCAAATAGTTTGAGTCTTCCTGGGCTGCAAATGGTAACGAAGAAGTTGGAATTGCCTGTGAAGATTGTCTTTCCTAGGAACTTATTGGGTGGAGAGATTCCTGGAGGGGCCAGAGACTTCATGATGCTTGGTCTCGGTGACATGGCAATTCCTTCCATGCTGCTGGCATTAGTCCTATGTTTTGATCATCGAAGGAGCAAGGATACGGTAAATCTGTTAGATATGCATTCCTCGAAGGGGCACAAATATATTTGGTATGCCCTTCCTGGATATGCCATTGGGCTGGTTACGGCTTTAGCAGCTGGCATCTTGACAAACTCTCCTCAACCTGCGCTTCTTTATCTGGTGCCTTCGACGCTGGGACCAATTGTTTTCATTTCTTGGATAAGGAAGGAACTAGCGGAGCTATGGGACGGACCCTTGCCAAACATGAACGATAAAGCTCACCAGATTGAAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005768 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005794 GO:0005798 GO:0005802 GO:0012505 GO:0016020 GO:0016021 GO:0030176 GO:0030660 GO:0031090 GO:0031224 GO:0031227 GO:0031410 GO:0031982 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071458 GO:0071556 GO:0097708 GO:0098552 GO:0098553 GO:0098554 GO:0098562 GO:0098588 GO:0098791 GO:0098805 GO:0098827 GO:0098852

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_A22B

Protein Analysis

371

Amino Acids

40.87

Weight (kDa)

8.41

Isoelectric Point (pI)

38.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_A22B	PF04258	48 - 356	8.4e-72	Signal peptide peptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014178)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33410
fragaria_vesca	FvH4_7g29650
malus_domestica	MD01G1203500.v1.1 MD07G1272800.v1.1
prunus_persica	Prupe.2G296100_v2.0.a1 Prupe.2G296100_v2.0.a1
pyrus_communis	pycom01g21260 pycom07g24920
rosa_chinensis	RchiOBHm_Chr1g0377661
rosa_laevigata	RLG00000026507
rosa_rugosa	Rorug01G0404000 Rorug01G0404100
rosa_samantha	Rh1AG428400 Rh1BG380900 Rh1CG394900 Rh1DG411900
rosa_wichuraiana	Rw1G037030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	988
AccB1I	GGYRCC	1 cut(s)	997
AccII	CGCG	1 cut(s)	379
AciI	CCGC	2 cut(s)	338, 1052
AclWI	GGATC	4 cut(s)	91, 175, 335, 348
AcsI	RAATTY	1 cut(s)	519
AfaI	GTAC	1 cut(s)	415
AfiI	CCNNNNNNNGG	1 cut(s)	888
AgsI	TTSAA	3 cut(s)	319, 454, 1106
AjnI	CCWGG	3 cut(s)	670, 751, 916
AjuI	GAANNNNNNNTTGG	2 cut(s)	446, 478
AluBI	AGCT	6 cut(s)	19, 195, 204, 953, 1057, 1094
AluI	AGCT	6 cut(s)	19, 195, 204, 953, 1057, 1094
Alw21I	GWGCWC	1 cut(s)	197
Alw26I	GTCTC	3 cut(s)	241, 759, 788
AlwI	GGATC	4 cut(s)	91, 175, 335, 348
AlwNI	CAGNNNCTG	1 cut(s)	422
AoxI	GGCC	1 cut(s)	759
ApeKI	GCWGC	5 cut(s)	308, 422, 676, 811, 950
ApoI	RAATTY	1 cut(s)	519
AspA2I	CCTAGG	1 cut(s)	725
AspLEI	GCGC	1 cut(s)	985
AspS9I	GGNCC	4 cut(s)	165, 759, 1013, 1067
AsuC2I	CCSGG	1 cut(s)	185
AsuHPI	GGTGA	3 cut(s)	199, 800, 1088
AvaII	GGWCC	3 cut(s)	165, 1013, 1067
AvrII	CCTAGG	1 cut(s)	725
BaeGI	GKGCMC	2 cut(s)	444, 894
BamHI	GGATCC	1 cut(s)	340
BanI	GGYRCC	1 cut(s)	997
BanII	GRGCYC	2 cut(s)	100, 197
BbsI	GAAGAC	2 cut(s)	497, 659
Bbv12I	GWGCWC	1 cut(s)	197
BbvI	GCAGC	5 cut(s)	320, 409, 663, 798, 962
BceAI	ACGGC	3 cut(s)	78, 248, 957
BciT130I	CCWGG	3 cut(s)	672, 753, 918
BciVI	GTATCC	1 cut(s)	847
BclI	TGATCA	1 cut(s)	835
BcnI	CCSGG	1 cut(s)	185
BcoDI	GTCTC	3 cut(s)	241, 759, 788
BfaI	CTAG	3 cut(s)	53, 726, 1049
BfuAI	ACCTGC	1 cut(s)	988
BfuI	GTATCC	1 cut(s)	847
BisI	GCNGC	5 cut(s)	309, 423, 677, 812, 951
BlnI	CCTAGG	1 cut(s)	725
BlsI	GCNGC	5 cut(s)	310, 424, 678, 813, 952
Bme1390I	CCNGG	4 cut(s)	185, 672, 753, 918
Bme18I	GGWCC	3 cut(s)	165, 1013, 1067
BmgT120I	GGNCC	4 cut(s)	165, 759, 1013, 1067
BmiI	GGNNCC	8 cut(s)	6, 36, 167, 342, 760, 999, 1014, 1069
BmrFI	CCNGG	4 cut(s)	185, 672, 753, 918
BmrI	ACTGGG	2 cut(s)	454, 632
BmsI	GCATC	2 cut(s)	765, 966
BmuI	ACTGGG	2 cut(s)	454, 632
BpiI	GAAGAC	2 cut(s)	497, 659
BpmI	CTGGAG	2 cut(s)	53, 774
BpuMI	CCSGG	1 cut(s)	185
BsaI	GGTCTC	1 cut(s)	788
BsaJI	CCNNGG	2 cut(s)	671, 725
BsaXI	ACNNNNNCTCC	2 cut(s)	955, 985
Bsc4I	CCNNNNNNNGG	1 cut(s)	888
Bse1I	ACTGG	2 cut(s)	449, 638
Bse3DI	GCAATG	1 cut(s)	534
BseBI	CCWGG	3 cut(s)	672, 753, 918
BseDI	CCNNGG	2 cut(s)	671, 725
BseLI	CCNNNNNNNGG	1 cut(s)	888
BseMI	GCAATG	1 cut(s)	534
BseMII	CTCAG	1 cut(s)	570
BseNI	ACTGG	2 cut(s)	449, 638
BseRI	GAGGAG	2 cut(s)	236, 963
BseSI	GKGCMC	2 cut(s)	444, 894
BseXI	GCAGC	5 cut(s)	320, 409, 663, 798, 962
BseYI	CCCAGC	1 cut(s)	1009
BsgI	GTGCAG	1 cut(s)	24
Bsh1236I	CGCG	1 cut(s)	379
BshFI	GGCC	1 cut(s)	761
BshNI	GGYRCC	1 cut(s)	997
BsiHKAI	GWGCWC	1 cut(s)	197
BsiSI	CCGG	1 cut(s)	185
BslFI	GGGAC	3 cut(s)	151, 1026, 1076
BslI	CCNNNNNNNGG	1 cut(s)	888
BsmAI	GTCTC	3 cut(s)	241, 759, 788
BsmBI	CGTCTC	1 cut(s)	241
BsmFI	GGGAC	3 cut(s)	151, 1026, 1076
BsmI	GAATGC	1 cut(s)	877
BsnI	GGCC	1 cut(s)	761
Bso31I	GGTCTC	1 cut(s)	788
Bsp1286I	GDGCHC	4 cut(s)	100, 197, 444, 894
Bsp143I	GATC	4 cut(s)	83, 180, 340, 835
BspACI	CCGC	2 cut(s)	338, 1052
BspANI	GGCC	1 cut(s)	761
BspCNI	CTCAG	1 cut(s)	571
BspFNI	CGCG	1 cut(s)	379
BspHI	TCATGA	2 cut(s)	177, 771
BspLI	GGNNCC	8 cut(s)	6, 36, 167, 342, 760, 999, 1014, 1069
BspMI	ACCTGC	1 cut(s)	988
BspPI	GGATC	4 cut(s)	91, 175, 335, 348
BspT107I	GGYRCC	1 cut(s)	997
BspTNI	GGTCTC	1 cut(s)	788
BsrDI	GCAATG	1 cut(s)	534
BsrI	ACTGG	2 cut(s)	449, 638
BssECI	CCNNGG	2 cut(s)	671, 725
BssMI	GATC	4 cut(s)	83, 180, 340, 835
BssT1I	CCWWGG	1 cut(s)	725
Bst2UI	CCWGG	3 cut(s)	672, 753, 918
Bst4CI	ACNGT	4 cut(s)	253, 418, 649, 859
BstC8I	GCNNGC	4 cut(s)	39, 206, 816, 955
BstDEI	CTNAG	1 cut(s)	579
BstFNI	CGCG	1 cut(s)	379
BstHHI	GCGC	1 cut(s)	985
BstKTI	GATC	4 cut(s)	86, 183, 343, 838
BstMAI	GTCTC	3 cut(s)	241, 759, 788
BstMBI	GATC	4 cut(s)	83, 180, 340, 835
BstMWI	GCNNNNNNNGC	4 cut(s)	95, 201, 950, 1006
BstNI	CCWGG	3 cut(s)	672, 753, 918
BstSCI	CCNGG	4 cut(s)	183, 670, 751, 916
BstSLI	GKGCMC	2 cut(s)	444, 894
BstUI	CGCG	1 cut(s)	379
BstV1I	GCAGC	5 cut(s)	320, 409, 663, 798, 962
BstV2I	GAAGAC	2 cut(s)	497, 659
BstX2I	RGATCY	2 cut(s)	83, 340
BstXI	CCANNNNNNTGG	2 cut(s)	814, 934
BstYI	RGATCY	2 cut(s)	83, 340
BsuI	GTATCC	1 cut(s)	847
BsuRI	GGCC	1 cut(s)	761
BtsIMutI	CAGTG	3 cut(s)	249, 442, 618
BveI	ACCTGC	1 cut(s)	988
Cac8I	GCNNGC	4 cut(s)	39, 206, 816, 955
CaiI	CAGNNNCTG	1 cut(s)	422
CciI	TCATGA	2 cut(s)	177, 771
CfoI	GCGC	1 cut(s)	985
Cfr13I	GGNCC	4 cut(s)	165, 759, 1013, 1067
CseI	GACGC	1 cut(s)	1015
Csp6I	GTAC	1 cut(s)	414
CviAII	CATG	8 cut(s)	131, 178, 497, 604, 772, 793, 808, 1081
CviQI	GTAC	1 cut(s)	414
DdeI	CTNAG	1 cut(s)	579
DpnI	GATC	4 cut(s)	85, 182, 342, 837
DpnII	GATC	4 cut(s)	83, 180, 340, 835
Ecl136II	GAGCTC	1 cut(s)	195
Eco130I	CCWWGG	1 cut(s)	725
Eco24I	GRGCYC	2 cut(s)	100, 197
Eco31I	GGTCTC	1 cut(s)	788
Eco47I	GGWCC	3 cut(s)	165, 1013, 1067
Eco53kI	GAGCTC	1 cut(s)	195
EcoICRI	GAGCTC	1 cut(s)	195
EcoO109I	RGGNCCY	1 cut(s)	165
EcoRII	CCWGG	3 cut(s)	670, 751, 916
EcoT14I	CCWWGG	1 cut(s)	725
EcoT22I	ATGCAT	2 cut(s)	410, 879
EcoT38I	GRGCYC	2 cut(s)	100, 197
ErhI	CCWWGG	1 cut(s)	725
Esp3I	CGTCTC	1 cut(s)	241
FaeI	CATG	8 cut(s)	134, 181, 500, 607, 775, 796, 811, 1084
FalI	AAGNNNNNCTT	2 cut(s)	704, 736
FaqI	GGGAC	3 cut(s)	151, 1026, 1076
FatI	CATG	8 cut(s)	130, 177, 496, 603, 771, 792, 807, 1080
FauNDI	CATATG	2 cut(s)	346, 476
FbaI	TGATCA	1 cut(s)	835
Fnu4HI	GCNGC	5 cut(s)	309, 423, 677, 812, 951
FriOI	GRGCYC	2 cut(s)	100, 197
Fsp4HI	GCNGC	5 cut(s)	309, 423, 677, 812, 951
FspBI	CTAG	3 cut(s)	53, 726, 1049
GlaI	GCGC	1 cut(s)	984
GluI	GCNGC	5 cut(s)	309, 423, 677, 812, 951
GsaI	CCCAGC	1 cut(s)	1013
GsuI	CTGGAG	2 cut(s)	53, 774
HaeIII	GGCC	1 cut(s)	761
HapII	CCGG	1 cut(s)	185
HgaI	GACGC	1 cut(s)	1015
HhaI	GCGC	1 cut(s)	985
Hin1II	CATG	8 cut(s)	134, 181, 500, 607, 775, 796, 811, 1084
Hin6I	GCGC	1 cut(s)	983
HinP1I	GCGC	1 cut(s)	983
HindIII	AAGCTT	2 cut(s)	17, 202
HinfI	GANTC	4 cut(s)	381, 493, 664, 748
HpaII	CCGG	1 cut(s)	185
HphI	GGTGA	3 cut(s)	199, 800, 1088
Hpy166II	GTNNAC	1 cut(s)	228
Hpy188I	TCNGA	2 cut(s)	142, 580
Hpy188III	TCNNGA	4 cut(s)	13, 178, 772, 961
Hpy8I	GTNNAC	1 cut(s)	228
Hpy99I	CGWCG	1 cut(s)	1009
HpyAV	CCTTC	6 cut(s)	813, 837, 880, 923, 1011, 1036
HpyCH4III	ACNGT	4 cut(s)	253, 418, 649, 859
HpyCH4IV	ACGT	1 cut(s)	155
HpyCH4V	TGCA	8 cut(s)	41, 89, 308, 408, 527, 653, 679, 877
HpyF10VI	GCNNNNNNNGC	4 cut(s)	95, 201, 950, 1006
HpyF3I	CTNAG	1 cut(s)	579
HpySE526I	ACGT	1 cut(s)	155
Hsp92II	CATG	8 cut(s)	134, 181, 500, 607, 775, 796, 811, 1084
HspAI	GCGC	1 cut(s)	983
Ksp22I	TGATCA	1 cut(s)	835
Kzo9I	GATC	4 cut(s)	83, 180, 340, 835
LmnI	GCTCC	5 cut(s)	4, 34, 537, 846, 1054
Lsp1109I	GCAGC	5 cut(s)	320, 409, 663, 798, 962
LweI	GCATC	2 cut(s)	765, 966
MaeI	CTAG	3 cut(s)	53, 726, 1049
MaeII	ACGT	1 cut(s)	155
MaeIII	GTNAC	6 cut(s)	58, 73, 321, 685, 788, 937
MalI	GATC	4 cut(s)	85, 182, 342, 837
MboI	GATC	4 cut(s)	83, 180, 340, 835
MboII	GAAGA	6 cut(s)	283, 497, 565, 659, 703, 724
MfeI	CAATTG	1 cut(s)	1017
MflI	RGATCY	2 cut(s)	83, 340
MhlI	GDGCHC	4 cut(s)	100, 197, 444, 894
MluCI	AATT	8 cut(s)	103, 150, 326, 457, 519, 701, 798, 1017
MlyI	GAGTC	2 cut(s)	502, 673
MmeI	TCCRAC	1 cut(s)	678
MnlI	CCTC	8 cut(s)	18, 257, 280, 289, 640, 749, 892, 984
Mph1103I	ATGCAT	2 cut(s)	410, 879
MspA1I	CMGCKG	1 cut(s)	953
MspI	CCGG	1 cut(s)	185
MspR9I	CCNGG	4 cut(s)	185, 672, 753, 918
MunI	CAATTG	1 cut(s)	1017
Mva1269I	GAATGC	1 cut(s)	877
MvaI	CCWGG	3 cut(s)	672, 753, 918
MvnI	CGCG	1 cut(s)	379
MwoI	GCNNNNNNNGC	4 cut(s)	95, 201, 950, 1006
NciI	CCSGG	1 cut(s)	185
NdeI	CATATG	2 cut(s)	346, 476
NdeII	GATC	4 cut(s)	83, 180, 340, 835
NlaIII	CATG	8 cut(s)	134, 181, 500, 607, 775, 796, 811, 1084
NlaIV	GGNNCC	8 cut(s)	6, 36, 167, 342, 760, 999, 1014, 1069
NmuCI	GTSAC	3 cut(s)	73, 321, 788
NsiI	ATGCAT	2 cut(s)	410, 879
PagI	TCATGA	2 cut(s)	177, 771
PctI	GAATGC	1 cut(s)	877
PfeI	GAWTC	2 cut(s)	381, 748
PfoI	TCCNGGA	2 cut(s)	751, 916
PkrI	GCNGC	5 cut(s)	310, 424, 678, 813, 952
PleI	GAGTC	2 cut(s)	501, 672
PpsI	GAGTC	2 cut(s)	501, 672
PpuMI	RGGWCCY	1 cut(s)	165
Psp124BI	GAGCTC	1 cut(s)	197
Psp5II	RGGWCCY	1 cut(s)	165
Psp6I	CCWGG	3 cut(s)	670, 751, 916
PspFI	CCCAGC	1 cut(s)	1009
PspGI	CCWGG	3 cut(s)	670, 751, 916
PspN4I	GGNNCC	8 cut(s)	6, 36, 167, 342, 760, 999, 1014, 1069
PspPI	GGNCC	4 cut(s)	165, 759, 1013, 1067
PspPPI	RGGWCCY	1 cut(s)	165
PstNI	CAGNNNCTG	1 cut(s)	422
PsuI	RGATCY	2 cut(s)	83, 340
PvuII	CAGCTG	1 cut(s)	953
RsaI	GTAC	1 cut(s)	415
RsaNI	GTAC	1 cut(s)	414
SacI	GAGCTC	1 cut(s)	197
SatI	GCNGC	5 cut(s)	309, 423, 677, 812, 951
Sau3AI	GATC	4 cut(s)	83, 180, 340, 835
Sau96I	GGNCC	4 cut(s)	165, 759, 1013, 1067
SchI	GAGTC	2 cut(s)	502, 673
ScrFI	CCNGG	4 cut(s)	185, 672, 753, 918
SduI	GDGCHC	4 cut(s)	100, 197, 444, 894
SfaNI	GCATC	2 cut(s)	765, 966
SinI	GGWCC	3 cut(s)	165, 1013, 1067
Sse9I	AATT	8 cut(s)	103, 150, 326, 457, 519, 701, 798, 1017
SsiI	CCGC	2 cut(s)	338, 1052
SspMI	CTAG	3 cut(s)	53, 726, 1049
SstI	GAGCTC	1 cut(s)	197
StyD4I	CCNGG	4 cut(s)	183, 670, 751, 916
StyI	CCWWGG	1 cut(s)	725
TaaI	ACNGT	4 cut(s)	253, 418, 649, 859
TaiI	ACGT	1 cut(s)	158
TaqI	TCGA	3 cut(s)	841, 884, 1004
TasI	AATT	8 cut(s)	103, 150, 326, 457, 519, 701, 798, 1017
TfiI	GAWTC	2 cut(s)	381, 748
TscAI	CASTG	3 cut(s)	256, 449, 618
TseFI	GTSAC	3 cut(s)	73, 321, 788
TseI	GCWGC	5 cut(s)	308, 422, 676, 811, 950
Tsp45I	GTSAC	3 cut(s)	73, 321, 788
TspDTI	ATGAA	4 cut(s)	632, 760, 1015, 1097
TspGWI	ACGGA	3 cut(s)	223, 262, 1080
TspRI	CASTG	3 cut(s)	256, 449, 618
VpaK11BI	GGWCC	3 cut(s)	165, 1013, 1067
XapI	RAATTY	1 cut(s)	519
XmaJI	CCTAGG	1 cut(s)	725
XspI	CTAG	3 cut(s)	53, 726, 1049
Zsp2I	ATGCAT	2 cut(s)	410, 879

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.