FvH4_7g29650

Signal peptide peptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014178
Physical Location & Seq
Forward (+)
21775787 .. 21778938
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29650.t1

Sequence Viewer

Length: 1116 bp
ATGGGTTCTATATGGAATCTTTTGTACCTGCTGGAGCCTGCACCTGTTTCTCTAATTCTAACGGCAGTAGGTGTTACTTTTGGGTCTGCATTTCGTGCTCTAAATTATGGAAAAGAAATGGAGCGGAATCGTGACTTTTCAGAAGCATCAATTACGTTAGATAGGTCCCAAGCACTCATGATCCCTGTAATGAGCTCCTTAAGCTTGCTTTTGATGTTCTACCTCTTCACTTCTGTTTCACAACTACTGACTGTATTCACAGCCGTTGCATCCGTCTCCTCCCTGTTCTTTTGCCTATCTCCTTCGGTAGCCTATTTAAAGTCTCAACTTGGTTTGGCTGACCCCTATGTGTCAAGGTGTTGTTCGAAGTCATTTACGCGCACCCAAGGATCATTACTGGTTTTATGCATCGGTACAGTTGCTGCCTGGCTTGTTACTGGACATTGGATTCTGAACAACTTGTTGGGTATTTCTATCTGTATTGCCTTTGTGAGTCATGTCCGTCTTCCAAACATCAAAGTGTGTGCTATGCTTCTTGTCTGTCTATTTGTATATGACATATTCTGGGTCTTCTTCTCTGAGAGATTTTTTGGGGCAAATGTCATGGTATCGGTAGCAACACAGCAAGCCTCAAACCCTGTGCACACGGTTGCAAATAGTTTGAGTCTTCCTGGGCTGCAAATGGTGACAAAGAAGTTGGAATTGCCTGTGAAGATTGTCTTTCCAAGAAACTTATTTGGTGGAGTAACTCCCGGAGGAAGTACAAGAGACTTCATGATGCTCGGTCTGGGTGACATGGCAATTCCTGCTATGCTGCTAGCATTAGTGCTATGTTTTGATTATCGAAAGAGCAGTAACACAGTAAATTTGCTAGATATGCATTCCTCAAAGGGGCACAAGTATATTTGGTATGCCCTTCCTGGATATGCCATTGGATTGGTCACAGCTTTAGCAGCTGGAGTTTTGACGCAGTCACCCCAACCGGCTCTTCTCTATTTGGTGCCCTCAACGCTAGGACCAATTGTCGTCATTTCTTGGATACGGAAGGAACTAGCAGAACTATGGGATGGCCCTTTGCCAAGCTTCAATGATAAAGTTCACCAGATAGAAGTATGA

Protein Analysis

372

Amino Acids

40.61

Weight (kDa)

8.74

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_A22B PF04258 49 - 357 1.2e-73 Signal peptide peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33410
fragaria_vesca FvH4_7g29650
malus_domestica MD01G1203500.v1.1 MD07G1272800.v1.1
prunus_persica Prupe.2G296100_v2.0.a1 Prupe.2G296100_v2.0.a1
pyrus_communis pycom01g21260 pycom07g24920
rosa_chinensis RchiOBHm_Chr1g0377661
rosa_laevigata RLG00000026507
rosa_rugosa Rorug01G0404000 Rorug01G0404100
rosa_samantha Rh1AG428400 Rh1BG380900 Rh1CG394900 Rh1DG411900
rosa_wichuraiana Rw1G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 36
AccB1I GGYRCC 1 cut(s) 1000
AccBSI CCGCTC 1 cut(s) 124
AccII CGCG 1 cut(s) 379
AciI CCGC 1 cut(s) 124
AclWI GGATC 2 cut(s) 175, 397
AcsI RAATTY 1 cut(s) 865
AfaI GTAC 3 cut(s) 26, 415, 763
AfiI CCNNNNNNNGG 1 cut(s) 891
AflII CTTAAG 1 cut(s) 199
AgsI TTSAA 1 cut(s) 1087
AhdI GACNNNNNGTC 1 cut(s) 1022
AjnI CCWGG 3 cut(s) 425, 670, 919
AjuI GAANNNNNNNTTGG 4 cut(s) 446, 478, 972, 1004
AluBI AGCT 5 cut(s) 195, 204, 947, 956, 1083
AluI AGCT 5 cut(s) 195, 204, 947, 956, 1083
Alw21I GWGCWC 3 cut(s) 100, 197, 645
Alw26I GTCTC 3 cut(s) 280, 327, 762
Alw44I GTGCAC 1 cut(s) 641
AlwI GGATC 2 cut(s) 175, 397
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 1 cut(s) 1069
ApaLI GTGCAC 1 cut(s) 641
ApeKI GCWGC 4 cut(s) 422, 676, 814, 953
ApoI RAATTY 1 cut(s) 865
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 3 cut(s) 165, 1016, 1070
AsuC2I CCSGG 1 cut(s) 753
AsuHPI GGTGA 4 cut(s) 697, 803, 966, 1091
AsuII TTCGAA 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 817
AvaII GGWCC 2 cut(s) 165, 1016
BaeGI GKGCMC 3 cut(s) 645, 897, 1005
BanI GGYRCC 1 cut(s) 1000
BanII GRGCYC 1 cut(s) 197
BbsI GAAGAC 3 cut(s) 497, 562, 659
Bbv12I GWGCWC 3 cut(s) 100, 197, 645
BbvI GCAGC 4 cut(s) 409, 663, 801, 965
BccI CCATC 1 cut(s) 1061
BceAI ACGGC 2 cut(s) 78, 248
BciT130I CCWGG 3 cut(s) 427, 672, 921
BciVI GTATCC 1 cut(s) 1032
BcnI CCSGG 1 cut(s) 753
BcoDI GTCTC 3 cut(s) 280, 327, 762
BfaI CTAG 4 cut(s) 818, 872, 1013, 1052
BfrI CTTAAG 1 cut(s) 199
BfuAI ACCTGC 1 cut(s) 36
BfuI GTATCC 1 cut(s) 1032
BisI GCNGC 4 cut(s) 423, 677, 815, 954
BlsI GCNGC 4 cut(s) 424, 678, 816, 955
Bme1390I CCNGG 4 cut(s) 427, 672, 753, 921
Bme18I GGWCC 2 cut(s) 165, 1016
BmeRI GACNNNNNGTC 1 cut(s) 1022
BmgT120I GGNCC 3 cut(s) 165, 1016, 1070
BmiI GGNNCC 3 cut(s) 36, 167, 1002
BmrFI CCNGG 4 cut(s) 427, 672, 753, 921
BmsI GCATC 4 cut(s) 155, 278, 417, 768
BmtI GCTAGC 1 cut(s) 821
BpiI GAAGAC 3 cut(s) 497, 562, 659
BpmI CTGGAG 2 cut(s) 53, 978
Bpu14I TTCGAA 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 753
BsaJI CCNNGG 2 cut(s) 385, 671
Bsc4I CCNNNNNNNGG 1 cut(s) 891
Bse118I RCCGGY 1 cut(s) 982
Bse1I ACTGG 2 cut(s) 402, 442
BseBI CCWGG 3 cut(s) 427, 672, 921
BseDI CCNNGG 2 cut(s) 385, 671
BseGI GGATG 2 cut(s) 269, 1072
BseLI CCNNNNNNNGG 1 cut(s) 891
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 2 cut(s) 402, 442
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 3 cut(s) 645, 897, 1005
BseXI GCAGC 4 cut(s) 409, 663, 801, 965
BsgI GTGCAG 1 cut(s) 24
Bsh1236I CGCG 1 cut(s) 379
BshFI GGCC 1 cut(s) 1071
BshNI GGYRCC 1 cut(s) 1000
BsiHKAI GWGCWC 3 cut(s) 100, 197, 645
BsiSI CCGG 2 cut(s) 753, 983
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 1 cut(s) 891
BsmAI GTCTC 3 cut(s) 280, 327, 762
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 1 cut(s) 1071
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 5 cut(s) 100, 197, 645, 897, 1005
Bsp143I GATC 2 cut(s) 180, 389
BspACI CCGC 1 cut(s) 124
BspANI GGCC 1 cut(s) 1071
BspCNI CTCAG 1 cut(s) 571
BspFNI CGCG 1 cut(s) 379
BspHI TCATGA 2 cut(s) 177, 774
BspLI GGNNCC 3 cut(s) 36, 167, 1002
BspMI ACCTGC 1 cut(s) 36
BspOI GCTAGC 1 cut(s) 821
BspPI GGATC 2 cut(s) 175, 397
BspQI GCTCTTC 1 cut(s) 993
BspT104I TTCGAA 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 1000
BspTI CTTAAG 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 982
BsrI ACTGG 2 cut(s) 402, 442
BssAI RCCGGY 1 cut(s) 982
BssECI CCNNGG 2 cut(s) 385, 671
BssMI GATC 2 cut(s) 180, 389
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 3 cut(s) 427, 672, 921
Bst4CI ACNGT 4 cut(s) 253, 418, 649, 862
Bst6I CTCTTC 2 cut(s) 230, 993
BstAFI CTTAAG 1 cut(s) 199
BstAPI GCANNNNNTGC 2 cut(s) 95, 806
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 4 cut(s) 39, 206, 627, 819
BstDEI CTNAG 1 cut(s) 579
BstF5I GGATG 2 cut(s) 269, 1072
BstFNI CGCG 1 cut(s) 379
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 2 cut(s) 183, 392
BstMAI GTCTC 3 cut(s) 280, 327, 762
BstMBI GATC 2 cut(s) 180, 389
BstMWI GCNNNNNNNGC 6 cut(s) 95, 201, 806, 877, 953, 1009
BstNI CCWGG 3 cut(s) 427, 672, 921
BstSCI CCNGG 4 cut(s) 425, 670, 751, 919
BstSLI GKGCMC 3 cut(s) 645, 897, 1005
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 4 cut(s) 409, 663, 801, 965
BstV2I GAAGAC 3 cut(s) 497, 562, 659
BstXI CCANNNNNNTGG 1 cut(s) 937
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 1 cut(s) 1071
BtsCI GGATG 2 cut(s) 269, 1072
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 4 cut(s) 39, 206, 627, 819
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 2 cut(s) 177, 774
CfoI GCGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 982
Cfr13I GGNCC 3 cut(s) 165, 1016, 1070
CseI GACGC 1 cut(s) 976
Csp6I GTAC 3 cut(s) 25, 414, 762
CviAII CATG 5 cut(s) 178, 497, 604, 775, 796
CviQI GTAC 3 cut(s) 25, 414, 762
DdeI CTNAG 1 cut(s) 579
DpnI GATC 2 cut(s) 182, 391
DpnII GATC 2 cut(s) 180, 389
DraI TTTAAA 1 cut(s) 318
DriI GACNNNNNGTC 1 cut(s) 1022
Eam1104I CTCTTC 2 cut(s) 230, 993
Eam1105I GACNNNNNGTC 1 cut(s) 1022
EarI CTCTTC 2 cut(s) 230, 993
Ecl136II GAGCTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 197
Eco47I GGWCC 2 cut(s) 165, 1016
Eco53kI GAGCTC 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 165
EcoRII CCWGG 3 cut(s) 425, 670, 919
EcoT14I CCWWGG 1 cut(s) 385
EcoT22I ATGCAT 2 cut(s) 410, 882
EcoT38I GRGCYC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 5 cut(s) 181, 500, 607, 778, 799
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 1 cut(s) 151
FatI CATG 5 cut(s) 177, 496, 603, 774, 795
Fnu4HI GCNGC 4 cut(s) 423, 677, 815, 954
FokI GGATG 2 cut(s) 256, 1079
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 4 cut(s) 423, 677, 815, 954
FspBI CTAG 4 cut(s) 818, 872, 1013, 1052
GlaI GCGC 1 cut(s) 380
GluI GCNGC 4 cut(s) 423, 677, 815, 954
GsuI CTGGAG 2 cut(s) 53, 978
HaeIII GGCC 1 cut(s) 1071
HapII CCGG 2 cut(s) 753, 983
HgaI GACGC 1 cut(s) 976
HhaI GCGC 1 cut(s) 381
Hin1II CATG 5 cut(s) 181, 500, 607, 778, 799
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HindIII AAGCTT 2 cut(s) 202, 1081
HinfI GANTC 5 cut(s) 16, 127, 448, 493, 664
HpaII CCGG 2 cut(s) 753, 983
HphI GGTGA 4 cut(s) 697, 803, 966, 1091
Hpy166II GTNNAC 2 cut(s) 643, 1099
Hpy188I TCNGA 3 cut(s) 142, 453, 580
Hpy188III TCNNGA 3 cut(s) 131, 178, 775
Hpy8I GTNNAC 2 cut(s) 643, 1099
HpyAV CCTTC 3 cut(s) 312, 926, 1039
HpyCH4III ACNGT 4 cut(s) 253, 418, 649, 862
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 8 cut(s) 41, 89, 269, 408, 643, 653, 679, 880
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 201, 806, 877, 953, 1009
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 5 cut(s) 181, 500, 607, 778, 799
HspAI GCGC 1 cut(s) 379
Kzo9I GATC 2 cut(s) 180, 389
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 3 cut(s) 34, 121, 200
Lsp1109I GCAGC 4 cut(s) 409, 663, 801, 965
LweI GCATC 4 cut(s) 155, 278, 417, 768
MaeI CTAG 4 cut(s) 818, 872, 1013, 1052
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 9 cut(s) 73, 131, 433, 685, 745, 791, 854, 940, 972
MalI GATC 2 cut(s) 182, 391
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 180, 389
MboII GAAGA 7 cut(s) 217, 497, 562, 565, 659, 724, 980
MfeI CAATTG 1 cut(s) 1020
MhlI GDGCHC 5 cut(s) 100, 197, 645, 897, 1005
MluCI AATT 7 cut(s) 54, 103, 150, 701, 801, 865, 1020
MlyI GAGTC 2 cut(s) 502, 673
MmeI TCCRAC 1 cut(s) 678
MnlI CCTC 6 cut(s) 233, 289, 640, 749, 895, 1015
Mph1103I ATGCAT 2 cut(s) 410, 882
MseI TTAA 2 cut(s) 200, 317
MslI CAYNNNNRTG 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 956
MspCI CTTAAG 1 cut(s) 199
MspI CCGG 2 cut(s) 753, 983
MspR9I CCNGG 4 cut(s) 427, 672, 753, 921
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 3 cut(s) 427, 672, 921
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 6 cut(s) 95, 201, 806, 877, 953, 1009
NciI CCSGG 1 cut(s) 753
NdeII GATC 2 cut(s) 180, 389
NheI GCTAGC 1 cut(s) 817
NlaIII CATG 5 cut(s) 181, 500, 607, 778, 799
NlaIV GGNNCC 3 cut(s) 36, 167, 1002
NmuCI GTSAC 5 cut(s) 131, 685, 791, 940, 972
NsiI ATGCAT 2 cut(s) 410, 882
NspV TTCGAA 1 cut(s) 365
PagI TCATGA 2 cut(s) 177, 774
PciSI GCTCTTC 1 cut(s) 993
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 3 cut(s) 16, 127, 448
PflFI GACNNNGTC 1 cut(s) 970
PfoI TCCNGGA 2 cut(s) 751, 919
PkrI GCNGC 4 cut(s) 424, 678, 816, 955
PleI GAGTC 2 cut(s) 501, 672
PpsI GAGTC 2 cut(s) 501, 672
PpuMI RGGWCCY 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 3 cut(s) 425, 670, 919
PspGI CCWGG 3 cut(s) 425, 670, 919
PspN4I GGNNCC 3 cut(s) 36, 167, 1002
PspPI GGNCC 3 cut(s) 165, 1016, 1070
PspPPI RGGWCCY 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 422
PsyI GACNNNGTC 1 cut(s) 970
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 3 cut(s) 26, 415, 763
RsaNI GTAC 3 cut(s) 25, 414, 762
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 2 cut(s) 200, 317
SatI GCNGC 4 cut(s) 423, 677, 815, 954
Sau3AI GATC 2 cut(s) 180, 389
Sau96I GGNCC 3 cut(s) 165, 1016, 1070
SchI GAGTC 2 cut(s) 502, 673
ScrFI CCNGG 4 cut(s) 427, 672, 753, 921
SduI GDGCHC 5 cut(s) 100, 197, 645, 897, 1005
SfaNI GCATC 4 cut(s) 155, 278, 417, 768
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 2 cut(s) 165, 1016
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 7 cut(s) 54, 103, 150, 701, 801, 865, 1020
SsiI CCGC 1 cut(s) 124
SspMI CTAG 4 cut(s) 818, 872, 1013, 1052
SstI GAGCTC 1 cut(s) 197
StyD4I CCNGG 4 cut(s) 425, 670, 751, 919
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 4 cut(s) 253, 418, 649, 862
TaiI ACGT 1 cut(s) 158
TaqI TCGA 2 cut(s) 365, 844
TaqII GACCGA 1 cut(s) 773
TasI AATT 7 cut(s) 54, 103, 150, 701, 801, 865, 1020
TatI WGTACW 1 cut(s) 761
TfiI GAWTC 3 cut(s) 16, 127, 448
Tru1I TTAA 2 cut(s) 200, 317
Tru9I TTAA 2 cut(s) 200, 317
TseFI GTSAC 5 cut(s) 131, 685, 791, 940, 972
TseI GCWGC 4 cut(s) 422, 676, 814, 953
Tsp45I GTSAC 5 cut(s) 131, 685, 791, 940, 972
TspDTI ATGAA 1 cut(s) 763
TspGWI ACGGA 3 cut(s) 262, 491, 1057
Tth111I GACNNNGTC 1 cut(s) 970
Vha464I CTTAAG 1 cut(s) 199
VneI GTGCAC 1 cut(s) 641
VpaK11BI GGWCC 2 cut(s) 165, 1016
XapI RAATTY 1 cut(s) 865
XspI CTAG 4 cut(s) 818, 872, 1013, 1052
Zsp2I ATGCAT 2 cut(s) 410, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.