RchiOBHm_Chr1g0377661

Signal peptide peptidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014178
Physical Location & Seq
Forward (+)
64466055 .. 64469050
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60110

Sequence Viewer

Length: 1116 bp
ATGGGGTCTATATGGAATCTTTTGTATTTGCTGGAGCCTGCACCTGTTTCTCTGATTTTAACGGCAGTAGGTGTTACTTTTGGGTCTGCATTTCGTGCTCTAAATTATGGAAAAGAAATGGAGCGGAATCGTGACTTTTCAGAAGCATCGATTACGTTAGATAGGTCACAAGCACTCATGATCCCTGTAATGAGCTCCCTAAGCTTGCTTTTGATGTTCTACCTCTTCACTTCTGTTTCACAACTCCTGACTGTATTCACAGCCATTGCATCCGTCTCCTCCCTGTTTTTCTGCCTATCTCCTTCAGTAGCCTATTTGAAGTCACAGCTTGGTTTGCCCGACCCCTATGTGTCGAGGTGTTGTTCGAAGTCGTTTACACGCACCCAAGGATCGTTACTGGTTTTATGCATTGGTACAGTTGCTGCTTGGCTTGTTACTGGACATTGGATACTGAACAACTTGTTGGGCATTTCTATATGTATTGCCTTTGTGAGTCATGTCCGTCTTCCAAATATCAAGGTTTGTGCAATGCTTCTTGTCTGTCTATTCGTATACGACATATTCTGGGTCTTCTTCTCTGAGAGATTTTTTGGGGCGAATGTCATGGTATCGGTAGCAACACAACAAGCCTCAAACCCCGTTCACACGGTGGCAAATAGTTTGAGTCTTCCTGGGCTGCAAATGGTGACAAAGAAGTTGGAGTTGCCCGTGAAGATTGTCTTCCCCAGGAACTTATTTGGTGGAGTGACTCCCGGAGGAAGTACCAGAGACTTCATGATGCTCGGTCTGGGTGACATGGCAATTCCTGCCATGCTTCTGGCATTAGTCCTATGTTTTGATTATCGAAAAAGCAGGGACACAGTAAATTTGCTAGATATGCATTCCTCAAAGGGGCACAAATATATTTGGTATGCCCTTCCTGGATATGCCTTTGGACTGGTCACAGCTTTAGCAGCTGGAGTTTTGACCCAGTCACCCCAACCTGCTCTTCTCTATTTGGTGCCCTCAACGCTAGGACCAATTGTCATCATTTCTTGGATTAGGAAGGAACTAGCAGAATTATGGGATGGCCCTTTGCCAAGCTTCAACGATAAAGTTCACCAGATAGAAGTATGA

Protein Analysis

371

Amino Acids

40.77

Weight (kDa)

8.74

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_A22B PF04258 49 - 357 2.2e-73 Signal peptide peptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014178)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G33410
fragaria_vesca FvH4_7g29650
malus_domestica MD01G1203500.v1.1 MD07G1272800.v1.1
prunus_persica Prupe.2G296100_v2.0.a1 Prupe.2G296100_v2.0.a1
pyrus_communis pycom01g21260 pycom07g24920
rosa_chinensis RchiOBHm_Chr1g0377661
rosa_laevigata RLG00000026507
rosa_rugosa Rorug01G0404000 Rorug01G0404100
rosa_samantha Rh1AG428400 Rh1BG380900 Rh1CG394900 Rh1DG411900
rosa_wichuraiana Rw1G037030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 991
AccB1I GGYRCC 1 cut(s) 1000
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 552
AciI CCGC 1 cut(s) 124
AclWI GGATC 2 cut(s) 175, 397
AcsI RAATTY 1 cut(s) 865
AcuI CTGAAG 1 cut(s) 288
AdeI CACNNNGTG 1 cut(s) 649
AfaI GTAC 2 cut(s) 415, 763
AfiI CCNNNNNNNGG 1 cut(s) 891
AgsI TTSAA 2 cut(s) 319, 1087
AhdI GACNNNNNGTC 1 cut(s) 1022
AjnI CCWGG 3 cut(s) 670, 725, 919
AjuI GAANNNNNNNTTGG 4 cut(s) 446, 478, 972, 1004
AluBI AGCT 6 cut(s) 195, 204, 328, 947, 956, 1083
AluI AGCT 6 cut(s) 195, 204, 328, 947, 956, 1083
Alw21I GWGCWC 2 cut(s) 100, 197
Alw26I GTCTC 2 cut(s) 280, 762
AlwI GGATC 2 cut(s) 175, 397
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 1 cut(s) 1069
ApeKI GCWGC 3 cut(s) 422, 676, 953
ApoI RAATTY 1 cut(s) 865
AspS9I GGNCC 2 cut(s) 1016, 1070
AsuC2I CCSGG 1 cut(s) 753
AsuHPI GGTGA 4 cut(s) 697, 803, 966, 1091
AsuII TTCGAA 1 cut(s) 365
AvaII GGWCC 1 cut(s) 1016
BaeGI GKGCMC 2 cut(s) 897, 1005
BanI GGYRCC 1 cut(s) 1000
BanII GRGCYC 1 cut(s) 197
BbsI GAAGAC 4 cut(s) 497, 562, 659, 712
Bbv12I GWGCWC 2 cut(s) 100, 197
BbvI GCAGC 3 cut(s) 409, 663, 965
BccI CCATC 1 cut(s) 1061
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 3 cut(s) 672, 727, 921
BciVI GTATCC 1 cut(s) 441
BcnI CCSGG 1 cut(s) 753
BcoDI GTCTC 2 cut(s) 280, 762
BfaI CTAG 3 cut(s) 872, 1013, 1052
BfuAI ACCTGC 1 cut(s) 991
BfuI GTATCC 1 cut(s) 441
BisI GCNGC 3 cut(s) 423, 677, 954
BlsI GCNGC 3 cut(s) 424, 678, 955
Bme1390I CCNGG 4 cut(s) 672, 727, 753, 921
Bme18I GGWCC 1 cut(s) 1016
BmeRI GACNNNNNGTC 1 cut(s) 1022
BmgT120I GGNCC 2 cut(s) 1016, 1070
BmiI GGNNCC 2 cut(s) 36, 1002
BmrFI CCNGG 4 cut(s) 672, 727, 753, 921
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 3 cut(s) 155, 278, 768
BmuI ACTGGG 1 cut(s) 964
BpiI GAAGAC 4 cut(s) 497, 562, 659, 712
BpmI CTGGAG 2 cut(s) 53, 978
Bpu10I CCTNAGC 1 cut(s) 200
Bpu14I TTCGAA 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 753
Bsa29I ATCGAT 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 385, 671, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 891
Bse1I ACTGG 4 cut(s) 402, 442, 942, 970
Bse3DI GCAATG 2 cut(s) 264, 534
BseBI CCWGG 3 cut(s) 672, 727, 921
BseCI ATCGAT 1 cut(s) 149
BseDI CCNNGG 3 cut(s) 385, 671, 725
BseGI GGATG 2 cut(s) 269, 1072
BseLI CCNNNNNNNGG 1 cut(s) 891
BseMI GCAATG 2 cut(s) 264, 534
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 4 cut(s) 402, 442, 942, 970
BseRI GAGGAG 1 cut(s) 268
BseSI GKGCMC 2 cut(s) 897, 1005
BseXI GCAGC 3 cut(s) 409, 663, 965
BsgI GTGCAG 1 cut(s) 24
BshFI GGCC 1 cut(s) 1071
BshNI GGYRCC 1 cut(s) 1000
BshVI ATCGAT 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 100, 197
BsiSI CCGG 1 cut(s) 753
BslFI GGGAC 1 cut(s) 869
BslI CCNNNNNNNGG 1 cut(s) 891
BsmAI GTCTC 2 cut(s) 280, 762
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 869
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 1 cut(s) 1071
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 4 cut(s) 100, 197, 897, 1005
Bsp143I GATC 2 cut(s) 180, 389
BspACI CCGC 1 cut(s) 124
BspANI GGCC 1 cut(s) 1071
BspCNI CTCAG 1 cut(s) 571
BspDI ATCGAT 1 cut(s) 149
BspHI TCATGA 2 cut(s) 177, 774
BspLI GGNNCC 2 cut(s) 36, 1002
BspMI ACCTGC 1 cut(s) 991
BspPI GGATC 2 cut(s) 175, 397
BspQI GCTCTTC 1 cut(s) 993
BspT104I TTCGAA 1 cut(s) 365
BspT107I GGYRCC 1 cut(s) 1000
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 2 cut(s) 264, 534
BsrI ACTGG 4 cut(s) 402, 442, 942, 970
BssECI CCNNGG 3 cut(s) 385, 671, 725
BssMI GATC 2 cut(s) 180, 389
BssNAI GTATAC 1 cut(s) 553
BssT1I CCWWGG 1 cut(s) 385
Bst1107I GTATAC 1 cut(s) 553
Bst2UI CCWGG 3 cut(s) 672, 727, 921
Bst4CI ACNGT 4 cut(s) 253, 418, 649, 862
Bst6I CTCTTC 2 cut(s) 230, 993
BstAPI GCANNNNNTGC 2 cut(s) 95, 806
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 39, 206
BstDEI CTNAG 2 cut(s) 200, 579
BstF5I GGATG 2 cut(s) 269, 1072
BstKTI GATC 2 cut(s) 183, 392
BstMAI GTCTC 2 cut(s) 280, 762
BstMBI GATC 2 cut(s) 180, 389
BstMWI GCNNNNNNNGC 7 cut(s) 95, 201, 334, 806, 877, 953, 1009
BstNI CCWGG 3 cut(s) 672, 727, 921
BstSCI CCNGG 4 cut(s) 670, 725, 751, 919
BstSLI GKGCMC 2 cut(s) 897, 1005
BstV1I GCAGC 3 cut(s) 409, 663, 965
BstV2I GAAGAC 4 cut(s) 497, 562, 659, 712
BstXI CCANNNNNNTGG 1 cut(s) 817
BstZ17I GTATAC 1 cut(s) 553
Bsu15I ATCGAT 1 cut(s) 149
BsuI GTATCC 1 cut(s) 441
BsuRI GGCC 1 cut(s) 1071
BsuTUI ATCGAT 1 cut(s) 149
BtsCI GGATG 2 cut(s) 269, 1072
BveI ACCTGC 1 cut(s) 991
Cac8I GCNNGC 2 cut(s) 39, 206
CaiI CAGNNNCTG 1 cut(s) 422
CciI TCATGA 2 cut(s) 177, 774
Cfr13I GGNCC 2 cut(s) 1016, 1070
ClaI ATCGAT 1 cut(s) 149
Csp6I GTAC 2 cut(s) 414, 762
CviAII CATG 6 cut(s) 178, 497, 604, 775, 796, 811
CviQI GTAC 2 cut(s) 414, 762
DdeI CTNAG 2 cut(s) 200, 579
DpnI GATC 2 cut(s) 182, 391
DpnII GATC 2 cut(s) 180, 389
DraIII CACNNNGTG 1 cut(s) 649
DriI GACNNNNNGTC 1 cut(s) 1022
Eam1104I CTCTTC 2 cut(s) 230, 993
Eam1105I GACNNNNNGTC 1 cut(s) 1022
EarI CTCTTC 2 cut(s) 230, 993
Ecl136II GAGCTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 1016
Eco53kI GAGCTC 1 cut(s) 195
Eco57I CTGAAG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 195
EcoRII CCWGG 3 cut(s) 670, 725, 919
EcoT14I CCWWGG 1 cut(s) 385
EcoT22I ATGCAT 2 cut(s) 410, 882
EcoT38I GRGCYC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 6 cut(s) 181, 500, 607, 778, 799, 814
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 1 cut(s) 869
FatI CATG 6 cut(s) 177, 496, 603, 774, 795, 810
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 3 cut(s) 423, 677, 954
FokI GGATG 2 cut(s) 256, 1079
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 423, 677, 954
FspBI CTAG 3 cut(s) 872, 1013, 1052
GluI GCNGC 3 cut(s) 423, 677, 954
GsuI CTGGAG 2 cut(s) 53, 978
HaeIII GGCC 1 cut(s) 1071
HapII CCGG 1 cut(s) 753
Hin1II CATG 6 cut(s) 181, 500, 607, 778, 799, 814
HindIII AAGCTT 2 cut(s) 202, 1081
HinfI GANTC 5 cut(s) 16, 127, 493, 664, 748
HpaII CCGG 1 cut(s) 753
HphI GGTGA 4 cut(s) 697, 803, 966, 1091
Hpy166II GTNNAC 4 cut(s) 375, 553, 643, 1099
Hpy188I TCNGA 3 cut(s) 54, 142, 580
Hpy188III TCNNGA 4 cut(s) 131, 178, 247, 775
Hpy8I GTNNAC 4 cut(s) 375, 553, 643, 1099
HpyAV CCTTC 3 cut(s) 312, 926, 1039
HpyCH4III ACNGT 4 cut(s) 253, 418, 649, 862
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 7 cut(s) 41, 89, 269, 408, 527, 679, 880
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 201, 334, 806, 877, 953, 1009
HpyF3I CTNAG 2 cut(s) 200, 579
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 6 cut(s) 181, 500, 607, 778, 799, 814
Kzo9I GATC 2 cut(s) 180, 389
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 3 cut(s) 34, 121, 200
Lsp1109I GCAGC 3 cut(s) 409, 663, 965
LweI GCATC 3 cut(s) 155, 278, 768
MaeI CTAG 3 cut(s) 872, 1013, 1052
MaeII ACGT 1 cut(s) 155
MalI GATC 2 cut(s) 182, 391
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 180, 389
MboII GAAGA 8 cut(s) 217, 497, 562, 565, 659, 712, 724, 980
MfeI CAATTG 1 cut(s) 1020
MhlI GDGCHC 4 cut(s) 100, 197, 897, 1005
MluCI AATT 5 cut(s) 103, 801, 865, 1020, 1058
MlyI GAGTC 3 cut(s) 502, 673, 742
MmeI TCCRAC 1 cut(s) 678
MnlI CCTC 7 cut(s) 233, 289, 348, 640, 749, 895, 1015
Mph1103I ATGCAT 2 cut(s) 410, 882
MseI TTAA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 956
MspI CCGG 1 cut(s) 753
MspR9I CCNGG 4 cut(s) 672, 727, 753, 921
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 3 cut(s) 672, 727, 921
MwoI GCNNNNNNNGC 7 cut(s) 95, 201, 334, 806, 877, 953, 1009
NciI CCSGG 1 cut(s) 753
NdeII GATC 2 cut(s) 180, 389
NlaIII CATG 6 cut(s) 181, 500, 607, 778, 799, 814
NlaIV GGNNCC 2 cut(s) 36, 1002
NmuCI GTSAC 8 cut(s) 131, 165, 321, 685, 745, 791, 940, 972
NsiI ATGCAT 2 cut(s) 410, 882
NspV TTCGAA 1 cut(s) 365
PagI TCATGA 2 cut(s) 177, 774
PciSI GCTCTTC 1 cut(s) 993
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 2 cut(s) 16, 127
PflFI GACNNNGTC 1 cut(s) 970
PfoI TCCNGGA 2 cut(s) 751, 919
PkrI GCNGC 3 cut(s) 424, 678, 955
PleI GAGTC 3 cut(s) 501, 672, 742
PpsI GAGTC 3 cut(s) 501, 672, 742
Psp124BI GAGCTC 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 670, 725, 919
PspGI CCWGG 3 cut(s) 670, 725, 919
PspN4I GGNNCC 2 cut(s) 36, 1002
PspPI GGNCC 2 cut(s) 1016, 1070
PstNI CAGNNNCTG 1 cut(s) 422
PsyI GACNNNGTC 1 cut(s) 970
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 2 cut(s) 415, 763
RsaNI GTAC 2 cut(s) 414, 762
SacI GAGCTC 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 3 cut(s) 423, 677, 954
Sau3AI GATC 2 cut(s) 180, 389
Sau96I GGNCC 2 cut(s) 1016, 1070
SchI GAGTC 3 cut(s) 502, 673, 742
ScrFI CCNGG 4 cut(s) 672, 727, 753, 921
SduI GDGCHC 4 cut(s) 100, 197, 897, 1005
SfaNI GCATC 3 cut(s) 155, 278, 768
SfuI TTCGAA 1 cut(s) 365
SinI GGWCC 1 cut(s) 1016
Sse9I AATT 5 cut(s) 103, 801, 865, 1020, 1058
SsiI CCGC 1 cut(s) 124
SspMI CTAG 3 cut(s) 872, 1013, 1052
SstI GAGCTC 1 cut(s) 197
StyD4I CCNGG 4 cut(s) 670, 725, 751, 919
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 4 cut(s) 253, 418, 649, 862
TaiI ACGT 1 cut(s) 158
TaqI TCGA 4 cut(s) 149, 353, 365, 844
TaqII GACCGA 1 cut(s) 773
TasI AATT 5 cut(s) 103, 801, 865, 1020, 1058
TfiI GAWTC 2 cut(s) 16, 127
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TseFI GTSAC 8 cut(s) 131, 165, 321, 685, 745, 791, 940, 972
TseI GCWGC 3 cut(s) 422, 676, 953
Tsp45I GTSAC 8 cut(s) 131, 165, 321, 685, 745, 791, 940, 972
TspDTI ATGAA 1 cut(s) 763
TspGWI ACGGA 2 cut(s) 262, 491
Tth111I GACNNNGTC 1 cut(s) 970
VpaK11BI GGWCC 1 cut(s) 1016
XapI RAATTY 1 cut(s) 865
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 3 cut(s) 872, 1013, 1052
Zsp2I ATGCAT 2 cut(s) 410, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.