AT4G34412

Belongs to the CGI121 TPRKB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007405
Physical Location & Seq
Reverse (-)
16452960 .. 16454633
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G34412.1

Sequence Viewer

Length: 519 bp
ATGAAGGTTTTTAATCTTGACCGTGGAAACACTCTCTCTGTTTCCCTCTTCTCCGGTGTCACCAACTCCAAGGAGCTCTTGAATTCAATGCTTGACGGAAGTCTGAAGCTGGAGGTCTCATTTCTCAATGCATCACTTATTCCAGACATTTTTCCTCTATTGGCTGCTGCTCAGAAAGCACTTATCTCCAAATCACGCGATTCCTTATCAACCAGAACTCTTCATTCTGAGCTTGTCTACAATTACTCAGGATCTAAGCACATTACAGAATCATTGAAACGATGTGGGATTTCTGAGAACACTACTTACATTCTAGCTGCCCGGTTCAATGCTTCTCCCGTTGAGATGGAGGAGGTGGCGAAATTGATCAACGGGAAGGAGATTGATTTGGAGGAATTGAAGACACATGCAAATCAAGCCAATATACTAAAGCATTACAAGATAACTAGCCAAGAGCTCGGAATTTCCTCTCTGGGAGATGCCATTGTTTGTCGAATAGCTGCACGCGATGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CGI-121

Protein Analysis

172

Amino Acids

18.83

Weight (kDa)

6.91

Isoelectric Point (pI)

29.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGI-121 PF08617 17 - 170 9.2e-43 Kinase binding protein CGI-121
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 237
AccII CGCG 2 cut(s) 198, 507
AclWI GGATC 1 cut(s) 259
AcsI RAATTY 2 cut(s) 82, 462
AcuI CTGAAG 1 cut(s) 125
AgsI TTSAA 5 cut(s) 82, 87, 277, 328, 400
AluBI AGCT 6 cut(s) 76, 109, 232, 317, 457, 500
AluI AGCT 6 cut(s) 76, 109, 232, 317, 457, 500
Alw21I GWGCWC 2 cut(s) 78, 459
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 259
ApeKI GCWGC 4 cut(s) 164, 167, 317, 500
ApoI RAATTY 2 cut(s) 82, 462
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 52
BanII GRGCYC 2 cut(s) 78, 459
BbsI GAAGAC 1 cut(s) 407
Bbv12I GWGCWC 2 cut(s) 78, 459
BbvI GCAGC 4 cut(s) 151, 154, 304, 487
BccI CCATC 1 cut(s) 340
BclI TGATCA 1 cut(s) 366
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 121
BfaI CTAG 2 cut(s) 314, 447
BisI GCNGC 4 cut(s) 165, 168, 318, 501
BlsI GCNGC 4 cut(s) 166, 169, 319, 502
Bme1390I CCNGG 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 3 cut(s) 140, 469, 499
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 407
BpmI CTGGAG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 322
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 2 cut(s) 22, 69
BsaWI WCCGGW 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 22, 69
BseMII CTCAG 4 cut(s) 185, 219, 261, 285
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 4 cut(s) 151, 154, 304, 487
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 2 cut(s) 198, 507
BsiHKAI GWGCWC 2 cut(s) 78, 459
BsiSI CCGG 2 cut(s) 54, 322
BsmAI GTCTC 1 cut(s) 121
Bso31I GGTCTC 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 78, 459
Bsp143I GATC 2 cut(s) 251, 366
BspCNI CTCAG 4 cut(s) 184, 220, 260, 286
BspFNI CGCG 2 cut(s) 198, 507
BspPI GGATC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 22, 69
BssMI GATC 2 cut(s) 251, 366
BssT1I CCWWGG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 23
Bst6I CTCTTC 2 cut(s) 53, 225
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 1 cut(s) 505
BstDEI CTNAG 5 cut(s) 171, 228, 247, 255, 294
BstDSI CCRYGG 1 cut(s) 22
BstFNI CGCG 2 cut(s) 198, 507
BstKTI GATC 2 cut(s) 254, 369
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 251, 366
BstMWI GCNNNNNNNGC 3 cut(s) 176, 416, 509
BstNSI RCATGY 1 cut(s) 410
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 320
BstUI CGCG 2 cut(s) 198, 507
BstV1I GCAGC 4 cut(s) 151, 154, 304, 487
BstV2I GAAGAC 1 cut(s) 407
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BtgI CCRYGG 1 cut(s) 22
Cac8I GCNNGC 1 cut(s) 505
CviAII CATG 1 cut(s) 407
CviJI RGCY 9 cut(s) 76, 109, 164, 232, 317, 419, 450, 457, 500
CviKI_1 RGCY 9 cut(s) 76, 109, 164, 232, 317, 419, 450, 457, 500
DdeI CTNAG 5 cut(s) 171, 228, 247, 255, 294
DpnI GATC 2 cut(s) 253, 368
DpnII GATC 2 cut(s) 251, 366
Eam1104I CTCTTC 2 cut(s) 53, 225
EarI CTCTTC 2 cut(s) 53, 225
Ecl136II GAGCTC 2 cut(s) 76, 457
Eco130I CCWWGG 1 cut(s) 69
Eco24I GRGCYC 2 cut(s) 78, 459
Eco31I GGTCTC 1 cut(s) 121
Eco53kI GAGCTC 2 cut(s) 76, 457
Eco57I CTGAAG 1 cut(s) 125
EcoICRI GAGCTC 2 cut(s) 76, 457
EcoRI GAATTC 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 69
EcoT22I ATGCAT 1 cut(s) 133
EcoT38I GRGCYC 2 cut(s) 78, 459
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 1 cut(s) 410
FaiI YATR 2 cut(s) 408, 425
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FatI CATG 1 cut(s) 406
FbaI TGATCA 1 cut(s) 366
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 4 cut(s) 165, 168, 318, 501
FriOI GRGCYC 2 cut(s) 78, 459
Fsp4HI GCNGC 4 cut(s) 165, 168, 318, 501
FspBI CTAG 2 cut(s) 314, 447
GluI GCNGC 4 cut(s) 165, 168, 318, 501
GsuI CTGGAG 1 cut(s) 131
HapII CCGG 2 cut(s) 54, 322
Hin1II CATG 1 cut(s) 410
HinfI GANTC 2 cut(s) 200, 269
HpaII CCGG 2 cut(s) 54, 322
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 5 cut(s) 105, 174, 229, 295, 461
Hpy188III TCNNGA 4 cut(s) 17, 79, 143, 249
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 131, 410, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 416, 509
HpyF3I CTNAG 5 cut(s) 171, 228, 247, 255, 294
Hsp92II CATG 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 2 cut(s) 251, 366
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 7 cut(s) 67, 95, 156, 226, 234, 335, 458
Lsp1109I GCAGC 4 cut(s) 151, 154, 304, 487
LweI GCATC 3 cut(s) 140, 469, 499
MaeI CTAG 2 cut(s) 314, 447
MaeIII GTNAC 1 cut(s) 58
MalI GATC 2 cut(s) 253, 368
MboI GATC 2 cut(s) 251, 366
MboII GAAGA 3 cut(s) 40, 212, 412
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 78, 459
MluCI AATT 5 cut(s) 82, 241, 362, 395, 462
MnlI CCTC 7 cut(s) 56, 106, 165, 343, 346, 385, 478
Mph1103I ATGCAT 1 cut(s) 133
MseI TTAA 1 cut(s) 12
MspI CCGG 2 cut(s) 54, 322
MspR9I CCNGG 1 cut(s) 322
MvnI CGCG 2 cut(s) 198, 507
MwoI GCNNNNNNNGC 3 cut(s) 176, 416, 509
NciI CCSGG 1 cut(s) 322
NdeII GATC 2 cut(s) 251, 366
NlaIII CATG 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 1 cut(s) 133
NspI RCATGY 1 cut(s) 410
PfeI GAWTC 2 cut(s) 200, 269
PkrI GCNGC 4 cut(s) 166, 169, 319, 502
PshAI GACNNNNGTC 1 cut(s) 99
Psp124BI GAGCTC 2 cut(s) 78, 459
PsrI GAACNNNNNNTAC 2 cut(s) 290, 322
PsuI RGATCY 1 cut(s) 251
SacI GAGCTC 2 cut(s) 78, 459
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 4 cut(s) 165, 168, 318, 501
Sau3AI GATC 2 cut(s) 251, 366
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 2 cut(s) 78, 459
SetI ASST 9 cut(s) 9, 78, 111, 117, 234, 319, 357, 459, 502
SfaNI GCATC 3 cut(s) 140, 469, 499
Sse9I AATT 5 cut(s) 82, 241, 362, 395, 462
SspMI CTAG 2 cut(s) 314, 447
SstI GAGCTC 2 cut(s) 78, 459
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 493
TasI AATT 5 cut(s) 82, 241, 362, 395, 462
TfiI GAWTC 2 cut(s) 200, 269
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 4 cut(s) 164, 167, 317, 500
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 2 cut(s) 17, 212
TspGWI ACGGA 1 cut(s) 111
XapI RAATTY 2 cut(s) 82, 462
XceI RCATGY 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 237
XspI CTAG 2 cut(s) 314, 447
Zsp2I ATGCAT 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.