MD05G1080500.v1.1

Belongs to the CGI121 TPRKB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007405
Physical Location & Seq
Forward (+)
16811926 .. 16813912
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1080500.v1.1.491

Sequence Viewer

Length: 582 bp
ATGAAGGAGTTTCAGGTCAATGGGAACACCCTCTCTCTTGCTCTCTTCACTGAAGTCACCAACTCCAAGGAGCTTCTTGATTTGATGCAATCAGGGACACTAAACCCGGAAGTTGCATTCCTTAATGCTTCACTTATCCCGGATGTTTTCCCTTTGCTTGCAGCTGCACATAAGACGCTCGTATCTAAGTCCCGAGAGTCATTGACGACGCGCACTCTTCATTCTGAGCTTGTTTACAATTACTCGGGATCCAAGCATATCACAGAGTCCTTGAAAAGATGTGGCATCTCTGAAAGCTCGACTTATGTGCTTGCCGCTCGTTTCAATGCTTCTTCTGAGGAGATAGAACAAAAGGCAGTAGAGAAACTTGTCAATGGAAAAGAGATTGACCTGGAGGAGTTAGCAGCGAGAGCAGATCAAGCCCAAATACAAAAGCACTATAAGATAACAAGCGTGGAGCTAGGGATATCGTCACTTGCCGATGCTATTACGTGCAGGATTGCTTCTCGCGATGCCTTGGAATCGAGGGCAGAATCGCAGAGCTATGTTTGTAAGGTGGCAAAGGAAACAGAAAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CGI-121

Protein Analysis

194

Amino Acids

21.23

Weight (kDa)

5.65

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGI-121 PF08617 16 - 171 2e-45 Kinase binding protein CGI-121
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 317
AccII CGCG 2 cut(s) 211, 510
AciI CCGC 1 cut(s) 315
AclWI GGATC 2 cut(s) 243, 256
AcuI CTGAAG 1 cut(s) 72
AgsI TTSAA 2 cut(s) 274, 325
AjnI CCWGG 1 cut(s) 390
AluBI AGCT 7 cut(s) 73, 164, 229, 297, 460, 543, 577
AluI AGCT 7 cut(s) 73, 164, 229, 297, 460, 543, 577
AlwI GGATC 2 cut(s) 243, 256
Ama87I CYCGRG 2 cut(s) 192, 244
ApeKI GCWGC 3 cut(s) 161, 164, 404
AspLEI GCGC 1 cut(s) 213
AsuC2I CCSGG 2 cut(s) 107, 140
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 2 cut(s) 192, 244
BaeI ACNNNNGTAYC 2 cut(s) 165, 198
BamHI GGATCC 1 cut(s) 248
BbvI GCAGC 3 cut(s) 151, 173, 416
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 1 cut(s) 392
BcnI CCSGG 2 cut(s) 107, 140
BfaI CTAG 1 cut(s) 461
BfmI CTRYAG 1 cut(s) 578
BisI GCNGC 4 cut(s) 162, 165, 315, 405
BlsI GCNGC 4 cut(s) 163, 166, 316, 406
Bme1390I CCNGG 3 cut(s) 107, 140, 392
BmeT110I CYCGRG 2 cut(s) 192, 244
BmiI GGNNCC 1 cut(s) 250
BmrFI CCNGG 3 cut(s) 107, 140, 392
BmsI GCATC 4 cut(s) 75, 294, 472, 502
BpmI CTGGAG 1 cut(s) 413
BpuMI CCSGG 2 cut(s) 107, 140
BsaAI YACGTR 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 66, 516
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 2 cut(s) 66, 516
BseGI GGATG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 216, 327
BseRI GAGGAG 2 cut(s) 353, 410
BseXI GCAGC 3 cut(s) 151, 173, 416
BsgI GTGCAG 2 cut(s) 150, 514
Bsh1236I CGCG 2 cut(s) 211, 510
BsiHKCI CYCGRG 2 cut(s) 192, 244
BsiSI CCGG 2 cut(s) 107, 140
BslFI GGGAC 2 cut(s) 109, 175
BsmFI GGGAC 2 cut(s) 109, 175
BsmI GAATGC 1 cut(s) 116
BsoBI CYCGRG 2 cut(s) 192, 244
Bsp143I GATC 2 cut(s) 248, 415
Bsp68I TCGCGA 1 cut(s) 510
BspACI CCGC 1 cut(s) 315
BspCNI CTCAG 2 cut(s) 217, 328
BspFNI CGCG 2 cut(s) 211, 510
BspLI GGNNCC 1 cut(s) 250
BspPI GGATC 2 cut(s) 243, 256
BsrBI CCGCTC 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 66, 516
BssMI GATC 2 cut(s) 248, 415
BssT1I CCWWGG 2 cut(s) 66, 516
Bst2UI CCWGG 1 cut(s) 392
Bst6I CTCTTC 2 cut(s) 50, 222
BstBAI YACGTR 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 159, 312
BstDEI CTNAG 3 cut(s) 186, 225, 336
BstF5I GGATG 1 cut(s) 148
BstFNI CGCG 2 cut(s) 211, 510
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 2 cut(s) 251, 418
BstMBI GATC 2 cut(s) 248, 415
BstMWI GCNNNNNNNGC 2 cut(s) 410, 419
BstNI CCWGG 1 cut(s) 392
BstSCI CCNGG 3 cut(s) 105, 138, 390
BstSFI CTRYAG 1 cut(s) 578
BstUI CGCG 2 cut(s) 211, 510
BstV1I GCAGC 3 cut(s) 151, 173, 416
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BtgZI GCGATG 1 cut(s) 525
BtsCI GGATG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 48
BtuMI TCGCGA 1 cut(s) 510
Cac8I GCNNGC 2 cut(s) 159, 312
CfoI GCGC 1 cut(s) 213
CseI GACGC 2 cut(s) 184, 217
CviJI RGCY 8 cut(s) 73, 164, 229, 297, 422, 460, 543, 577
CviKI_1 RGCY 8 cut(s) 73, 164, 229, 297, 422, 460, 543, 577
DdeI CTNAG 3 cut(s) 186, 225, 336
DpnI GATC 2 cut(s) 250, 417
DpnII GATC 2 cut(s) 248, 415
Eam1104I CTCTTC 2 cut(s) 50, 222
EarI CTCTTC 2 cut(s) 50, 222
Eco130I CCWWGG 2 cut(s) 66, 516
Eco32I GATATC 1 cut(s) 468
Eco57I CTGAAG 1 cut(s) 72
Eco88I CYCGRG 2 cut(s) 192, 244
EcoRII CCWGG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 66, 516
ErhI CCWWGG 2 cut(s) 66, 516
FaiI YATR 5 cut(s) 171, 258, 306, 441, 546
FalI AAGNNNNNCTT 2 cut(s) 286, 318
FaqI GGGAC 2 cut(s) 109, 175
Fnu4HI GCNGC 4 cut(s) 162, 165, 315, 405
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 4 cut(s) 162, 165, 315, 405
FspBI CTAG 1 cut(s) 461
GlaI GCGC 1 cut(s) 212
GluI GCNGC 4 cut(s) 162, 165, 315, 405
GsuI CTGGAG 1 cut(s) 413
HapII CCGG 2 cut(s) 107, 140
HgaI GACGC 2 cut(s) 184, 217
HhaI GCGC 1 cut(s) 213
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 4 cut(s) 197, 266, 521, 533
HpaII CCGG 2 cut(s) 107, 140
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 3 cut(s) 226, 292, 337
Hpy188III TCNNGA 4 cut(s) 77, 192, 246, 509
Hpy8I GTNNAC 1 cut(s) 235
Hpy99I CGWCG 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 5 cut(s) 88, 116, 161, 167, 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 419
HpyF3I CTNAG 3 cut(s) 186, 225, 336
HpySE526I ACGT 1 cut(s) 491
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 248, 415
LmnI GCTCC 2 cut(s) 70, 457
LpnPI CCDG 6 cut(s) 78, 120, 153, 377, 404, 481
Lsp1109I GCAGC 3 cut(s) 151, 173, 416
LweI GCATC 4 cut(s) 75, 294, 472, 502
MaeI CTAG 1 cut(s) 461
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 2 cut(s) 55, 471
MalI GATC 2 cut(s) 250, 417
MbiI CCGCTC 1 cut(s) 317
MboI GATC 2 cut(s) 248, 415
MboII GAAGA 3 cut(s) 37, 209, 324
MflI RGATCY 1 cut(s) 248
MluCI AATT 1 cut(s) 238
MlyI GAGTC 2 cut(s) 206, 275
MnlI CCTC 4 cut(s) 41, 331, 388, 519
MseI TTAA 1 cut(s) 123
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 2 cut(s) 107, 140
MspR9I CCNGG 3 cut(s) 107, 140, 392
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 2 cut(s) 211, 510
MwoI GCNNNNNNNGC 2 cut(s) 410, 419
NciI CCSGG 2 cut(s) 107, 140
NdeII GATC 2 cut(s) 248, 415
NlaIV GGNNCC 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 55, 471
NruI TCGCGA 1 cut(s) 510
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 521, 533
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 4 cut(s) 163, 166, 316, 406
PleI GAGTC 2 cut(s) 205, 274
PpsI GAGTC 2 cut(s) 205, 274
Ppu21I YACGTR 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 390
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 250
PsuI RGATCY 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 164
RruI TCGCGA 1 cut(s) 510
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 4 cut(s) 162, 165, 315, 405
Sau3AI GATC 2 cut(s) 248, 415
SchI GAGTC 2 cut(s) 206, 275
ScrFI CCNGG 3 cut(s) 107, 140, 392
SfaNI GCATC 4 cut(s) 75, 294, 472, 502
SfcI CTRYAG 1 cut(s) 578
Sse9I AATT 1 cut(s) 238
SsiI CCGC 1 cut(s) 315
SspMI CTAG 1 cut(s) 461
StyD4I CCNGG 3 cut(s) 105, 138, 390
StyI CCWWGG 2 cut(s) 66, 516
TaiI ACGT 1 cut(s) 494
TaqI TCGA 2 cut(s) 299, 524
TasI AATT 1 cut(s) 238
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 2 cut(s) 521, 533
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 2 cut(s) 55, 471
TseI GCWGC 3 cut(s) 161, 164, 404
Tsp45I GTSAC 2 cut(s) 55, 471
TspDTI ATGAA 2 cut(s) 17, 209
TspRI CASTG 1 cut(s) 55
XspI CTAG 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.