MD10G1094500.v1.1

Belongs to the CGI121 TPRKB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007405
Physical Location & Seq
Forward (+)
14692456 .. 14697359
4904 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1094500.v1.1.491

Sequence Viewer

Length: 393 bp
ATGAAGGACTTTCAGGGACTGCTTGATTTGATGCAATCAAGGACACTAAACGCAGAAGTCGCATTCCTGAATGCTTCGCTTATCCCAGATGTTTTCCCTTTACTTGCAGCTGCACATAAGACTCTTGTATCCAAGTCCCGAGAGTCACTGACAACACGCACTCTTCATTCTGAGCTTGTTTACAATTACTCAGGATCCAAGCATATCACAGAGTCCTTGAAAAGATGTGGGATTTCTGAAAGCTCAACCTATGTGCTTGCCGCTCGTTTCAATGCTTCTTTTGATGAGATAAGATCACTGTCTCATGAAATGCCTGGTAGATCAAGCGTTACATTGCTTCAGTATATCCGAAAATTGTTAGCACTTTCGACTTTAATCATGTTCATGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CGI-121

Protein Analysis

131

Amino Acids

14.56

Weight (kDa)

9.2

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGI-121 PF08617 7 - 102 2.8e-26 Kinase binding protein CGI-121
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 263
AciI CCGC 1 cut(s) 261
AclWI GGATC 2 cut(s) 189, 202
AcuI CTGAAG 1 cut(s) 323
AgsI TTSAA 2 cut(s) 220, 271
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 3 cut(s) 110, 175, 243
AluI AGCT 3 cut(s) 110, 175, 243
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 2 cut(s) 189, 202
AlwNI CAGNNNCTG 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 107, 110
AvaI CYCGRG 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BamHI GGATCC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 97, 119
BciT130I CCWGG 1 cut(s) 315
BciVI GTATCC 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 306
BfuI GTATCC 1 cut(s) 139
BisI GCNGC 3 cut(s) 108, 111, 261
BlsI GCNGC 3 cut(s) 109, 112, 262
Bme1390I CCNGG 1 cut(s) 315
BmeT110I CYCGRG 1 cut(s) 138
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 332
BseBI CCWGG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 332
BseMII CTCAG 2 cut(s) 162, 204
BseXI GCAGC 2 cut(s) 97, 119
BsgI GTGCAG 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 138
BslFI GGGAC 2 cut(s) 30, 121
BsmAI GTCTC 1 cut(s) 306
BsmFI GGGAC 2 cut(s) 30, 121
BsmI GAATGC 2 cut(s) 62, 76
BsoBI CYCGRG 1 cut(s) 138
Bsp143I GATC 3 cut(s) 194, 293, 320
BspACI CCGC 1 cut(s) 261
BspCNI CTCAG 2 cut(s) 163, 203
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 196
BspPI GGATC 2 cut(s) 189, 202
BsrBI CCGCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 332
BssMI GATC 3 cut(s) 194, 293, 320
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 1 cut(s) 300
Bst6I CTCTTC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 2 cut(s) 171, 190
BstKTI GATC 3 cut(s) 197, 296, 323
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 3 cut(s) 194, 293, 320
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstV1I GCAGC 2 cut(s) 97, 119
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BsuI GTATCC 1 cut(s) 139
BtsIMutI CAGTG 2 cut(s) 146, 296
Cac8I GCNNGC 1 cut(s) 258
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 304
CviAII CATG 3 cut(s) 305, 379, 385
CviJI RGCY 3 cut(s) 110, 175, 243
CviKI_1 RGCY 3 cut(s) 110, 175, 243
DdeI CTNAG 2 cut(s) 171, 190
DpnI GATC 3 cut(s) 196, 295, 322
DpnII GATC 3 cut(s) 194, 293, 320
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco57I CTGAAG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 313
FaeI CATG 3 cut(s) 308, 382, 388
FaiI YATR 8 cut(s) 117, 204, 252, 306, 345, 380, 386, 391
FaqI GGGAC 2 cut(s) 30, 121
FatI CATG 3 cut(s) 304, 378, 384
Fnu4HI GCNGC 3 cut(s) 108, 111, 261
Fsp4HI GCNGC 3 cut(s) 108, 111, 261
GluI GCNGC 3 cut(s) 108, 111, 261
Hin1II CATG 3 cut(s) 308, 382, 388
HinfI GANTC 3 cut(s) 121, 143, 212
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 3 cut(s) 172, 238, 350
Hpy188III TCNNGA 4 cut(s) 67, 138, 192, 305
Hpy8I GTNNAC 1 cut(s) 181
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 34, 107, 113
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 171, 190
Hsp92II CATG 3 cut(s) 308, 382, 388
Kzo9I GATC 3 cut(s) 194, 293, 320
LpnPI CCDG 5 cut(s) 80, 99, 177, 300, 327
Lsp1109I GCAGC 2 cut(s) 97, 119
LweI GCATC 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 144, 328
MalI GATC 3 cut(s) 196, 295, 322
MbiI CCGCTC 1 cut(s) 263
MboI GATC 3 cut(s) 194, 293, 320
MboII GAAGA 1 cut(s) 155
MflI RGATCY 1 cut(s) 194
MluCI AATT 2 cut(s) 184, 353
MlyI GAGTC 3 cut(s) 115, 152, 221
MseI TTAA 1 cut(s) 374
MslI CAYNNNNRTG 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 315
Mva1269I GAATGC 2 cut(s) 62, 76
MvaI CCWGG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeII GATC 3 cut(s) 194, 293, 320
NlaIII CATG 3 cut(s) 308, 382, 388
NlaIV GGNNCC 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 144
PagI TCATGA 1 cut(s) 304
PctI GAATGC 2 cut(s) 62, 76
PkrI GCNGC 3 cut(s) 109, 112, 262
PleI GAGTC 3 cut(s) 115, 151, 220
PpsI GAGTC 3 cut(s) 115, 151, 220
PsiI TTATAA 1 cut(s) 391
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 194
PvuII CAGCTG 1 cut(s) 110
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 374
SatI GCNGC 3 cut(s) 108, 111, 261
Sau3AI GATC 3 cut(s) 194, 293, 320
SchI GAGTC 3 cut(s) 115, 152, 221
ScrFI CCNGG 1 cut(s) 315
SetI ASST 4 cut(s) 112, 177, 245, 251
SfaNI GCATC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 383
Sse9I AATT 2 cut(s) 184, 353
SsiI CCGC 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 300
TaqI TCGA 1 cut(s) 368
TasI AATT 2 cut(s) 184, 353
TauI GCSGC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 2 cut(s) 153, 303
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 2 cut(s) 107, 110
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 4 cut(s) 17, 155, 321, 373
TspRI CASTG 2 cut(s) 153, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.