AT4G35190
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013802
Physical Location & Seq
Forward (+)
16746189 .. 16748455
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35190.2

Sequence Viewer

Length: 687 bp
ATGGAAATAGTGAAGTCGAGGTTCAAGAGGGTTTGTGTGTTCTGTGGTAGCAGCAGCGGAAAGAGAGAGTGCTACAGTGATGCCGCCACTGATCTAGCTCAAGAGCTGGTGACGAGGAGATTGAATCTTGTGTATGGAGGAGGAAGCATTGGTCTCATGGGTTTGGTCTCACAAGCTGTTCATGAAGCTGGAGGACATGTACTAGGGATCATACCAAGGACTCTTATGGACAAAGAGATAACCGGAGAAACATATGGTGAGGTAATAGCTGTGGCGGATATGCATGAAAGAAAAGCCGAAATGGCACGCCACTCGGATTGTTTCATTGCTTTACCAGGTGGGTATGGAACACTGGAGGAGTTATTGGAAGTAATAGCATGGGCACAACTTGGAATTCACGACAAGCCTGTGGGTTTGTTAAATGTGGATGGTTATTACAATTACCTCCTCACTTTCATTGATAAAGCCGTTGATGATGGCTTTATCAAACCATCTCAGCGTCACATCTTTGTCTCAGCCCCCAATGCCAAAGAGCTTGTCCAAAAACTTGAGGCATACAAGCCAGTGAATGATGGAGTCATAGCTAAATCTAGGTGGGAGGTTGAGAAGAAAGTGCAACAGCCGCAACAACAGCAACAAGTAGTGTTCTGTTCTAACACAAGCATGCAGACTGAGATTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

228

Amino Acids

25.19

Weight (kDa)

5.89

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 16 - 141 3.5e-16 SLOG cluster4 family
Lysine_decarbox PF03641 53 - 183 4.5e-50 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35190 AT4G35190
malus_domestica MD05G1130400.v1.1 MD10G1133300.v1.1
prunus_persica Prupe.8G174600_v2.0.a1
pyrus_communis pycom05g12710
rosa_chinensis RchiOBHm_Chr6g0279791
rosa_laevigata RLG00000013119
rosa_multiflora Rmu_sc0002340.1_g000021
rosa_roxburghii Rroxscaffold_7G00188650
rosa_rugosa Rorug06G0128200 Rorug06G0128300
rosa_samantha Rh6AG236400 Rh6BG240500 Rh6CG242800 Rh6DG234200
rosa_wichuraiana Rw6G020600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 57, 84, 275, 623
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 393
AfaI GTAC 1 cut(s) 201
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 2 cut(s) 25, 124
AjnI CCWGG 1 cut(s) 334
AluBI AGCT 7 cut(s) 98, 106, 176, 188, 269, 535, 584
AluI AGCT 7 cut(s) 98, 106, 176, 188, 269, 535, 584
Alw26I GTCTC 3 cut(s) 158, 172, 517
AlwI GGATC 1 cut(s) 215
ApeKI GCWGC 2 cut(s) 51, 54
ApoI RAATTY 1 cut(s) 393
ArsI GACNNNNNNTTYG 2 cut(s) 522, 554
AsuHPI GGTGA 2 cut(s) 121, 269
BaeGI GKGCMC 1 cut(s) 385
BbvI GCAGC 2 cut(s) 63, 66
BccI CCATC 4 cut(s) 422, 470, 499, 566
BceAI ACGGC 1 cut(s) 452
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 1 cut(s) 336
BcoDI GTCTC 3 cut(s) 158, 172, 517
BfaI CTAG 3 cut(s) 95, 203, 591
BfmI CTRYAG 1 cut(s) 73
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 4 cut(s) 52, 55, 84, 623
BlsI GCNGC 4 cut(s) 53, 56, 85, 624
Bme1390I CCNGG 1 cut(s) 336
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 1 cut(s) 70
BpmI CTGGAG 2 cut(s) 210, 374
BpuEI CTTGAG 2 cut(s) 84, 569
BsaI GGTCTC 2 cut(s) 158, 172
BsaJI CCNNGG 1 cut(s) 215
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bse1I ACTGG 2 cut(s) 357, 563
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 1 cut(s) 433
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 509, 528, 663
BseNI ACTGG 2 cut(s) 357, 563
BseRI GAGGAG 4 cut(s) 130, 153, 371, 437
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 2 cut(s) 63, 66
BsiSI CCGG 1 cut(s) 243
BsmAI GTCTC 3 cut(s) 158, 172, 517
Bso31I GGTCTC 2 cut(s) 158, 172
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 2 cut(s) 91, 207
BspACI CCGC 4 cut(s) 57, 84, 275, 623
BspCNI CTCAG 3 cut(s) 508, 527, 664
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 215
BspTNI GGTCTC 2 cut(s) 158, 172
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 2 cut(s) 357, 563
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 2 cut(s) 91, 207
BssT1I CCWWGG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 307, 665
BstDEI CTNAG 3 cut(s) 495, 514, 672
BstF5I GGATG 1 cut(s) 433
BstKTI GATC 2 cut(s) 94, 210
BstMAI GTCTC 3 cut(s) 158, 172, 517
BstMBI GATC 2 cut(s) 91, 207
BstMWI GCNNNNNNNGC 4 cut(s) 302, 524, 622, 631
BstNI CCWGG 1 cut(s) 336
BstNSI RCATGY 2 cut(s) 200, 667
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 73
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 63, 66
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 4 cut(s) 82, 87, 350, 570
Cac8I GCNNGC 2 cut(s) 307, 665
CciI TCATGA 1 cut(s) 181
CseI GACGC 1 cut(s) 488
CsiI ACCWGGT 1 cut(s) 334
Csp6I GTAC 1 cut(s) 200
CspCI CAANNNNNGTGG 2 cut(s) 299, 334
CviAII CATG 6 cut(s) 157, 182, 197, 284, 378, 664
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 495, 514, 672
DpnI GATC 2 cut(s) 93, 209
DpnII GATC 2 cut(s) 91, 207
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 215
Eco31I GGTCTC 2 cut(s) 158, 172
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 215
EcoT22I ATGCAT 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 6 cut(s) 160, 185, 200, 287, 381, 667
FatI CATG 6 cut(s) 156, 181, 196, 283, 377, 663
FauNDI CATATG 1 cut(s) 253
Fnu4HI GCNGC 4 cut(s) 52, 55, 84, 623
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 4 cut(s) 52, 55, 84, 623
FspBI CTAG 3 cut(s) 95, 203, 591
GluI GCNGC 4 cut(s) 52, 55, 84, 623
GsuI CTGGAG 2 cut(s) 210, 374
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 488
Hin1II CATG 6 cut(s) 160, 185, 200, 287, 381, 667
HinfI GANTC 3 cut(s) 124, 220, 576
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 121, 269
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 4 cut(s) 25, 101, 182, 398
HpyCH4III ACNGT 1 cut(s) 77
HpyCH4V TGCA 3 cut(s) 283, 616, 667
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 524, 622, 631
HpyF3I CTNAG 3 cut(s) 495, 514, 672
Hsp92II CATG 6 cut(s) 160, 185, 200, 287, 381, 667
Kzo9I GATC 2 cut(s) 91, 207
LpnPI CCDG 8 cut(s) 92, 174, 256, 321, 338, 348, 420, 576
Lsp1109I GCAGC 2 cut(s) 63, 66
LweI GCATC 1 cut(s) 70
MabI ACCWGGT 1 cut(s) 334
MaeI CTAG 3 cut(s) 95, 203, 591
MaeIII GTNAC 2 cut(s) 109, 500
MalI GATC 2 cut(s) 93, 209
MboI GATC 2 cut(s) 91, 207
MboII GAAGA 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 2 cut(s) 393, 439
MlyI GAGTC 2 cut(s) 214, 585
Mph1103I ATGCAT 1 cut(s) 285
MseI TTAA 1 cut(s) 419
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 57
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 302, 524, 622, 631
NdeI CATATG 1 cut(s) 253
NdeII GATC 2 cut(s) 91, 207
NlaIII CATG 6 cut(s) 160, 185, 200, 287, 381, 667
NmuCI GTSAC 2 cut(s) 109, 500
NsiI ATGCAT 1 cut(s) 285
NspI RCATGY 2 cut(s) 200, 667
PaeI GCATGC 1 cut(s) 667
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 196
PfeI GAWTC 1 cut(s) 124
PkrI GCNGC 4 cut(s) 53, 56, 85, 624
PleI GAGTC 2 cut(s) 214, 584
PpsI GAGTC 2 cut(s) 214, 584
PscI ACATGT 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 662
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 4 cut(s) 52, 55, 84, 623
Sau3AI GATC 2 cut(s) 91, 207
SchI GAGTC 2 cut(s) 214, 585
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 1 cut(s) 385
SexAI ACCWGGT 1 cut(s) 334
SfaNI GCATC 1 cut(s) 70
SfcI CTRYAG 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 2 cut(s) 99, 548
SmoI CTYRAG 2 cut(s) 99, 548
SphI GCATGC 1 cut(s) 667
Sse9I AATT 2 cut(s) 393, 439
SsiI CCGC 4 cut(s) 57, 84, 275, 623
SspMI CTAG 3 cut(s) 95, 203, 591
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 1 cut(s) 77
TaqI TCGA 1 cut(s) 17
TasI AATT 2 cut(s) 393, 439
TatI WGTACW 1 cut(s) 199
TauI GCSGC 2 cut(s) 86, 625
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 4 cut(s) 82, 94, 357, 570
TseFI GTSAC 2 cut(s) 109, 500
TseI GCWGC 2 cut(s) 51, 54
Tsp45I GTSAC 2 cut(s) 109, 500
TspDTI ATGAA 5 cut(s) 170, 198, 300, 313, 445
TspRI CASTG 4 cut(s) 82, 94, 357, 570
XapI RAATTY 1 cut(s) 393
XceI RCATGY 2 cut(s) 200, 667
XspI CTAG 3 cut(s) 95, 203, 591
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.