Rorug06G0128300
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013802
Physical Location & Seq
Forward (+)
17811063 .. 17813655
2593 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0128300.1

Sequence Viewer

Length: 1203 bp
ATGAATGATCTAATGACCAAGTCGTTCCTCAGTTACGTTGATCTTAAGAAACAGGCCATGAAAGACATTGAAGCAGAACCTGACTTGGAAATGGGGAAACTTGAGCCTGCTGAAGAACAGAATCTTGCGAAATTTTTTGAAGAAGTGACGGCAATCAAGGCTGACATGGAAGAGATTACGAACCTGCTTCTTGATCTTCAGGACCTGAATGAAGAAACCAAGTCCACTCACAGTGCTAAAGTTCTCAGGGGGCTGAGAGACCGAATCAATGCTGACATGGTTAGTATTCTGAGGAAAGCTAAGAACATTAAAGCAAGATTGGAATCGCTTGATCAGTCTAATATTGCTAACCGAGAGGTGGCTGGGTACAAGGAAGGAAGTCCTGTTGATCGAATGAGGATTTCTGTGACTGGTGGACTGAGAATCAAGCTGAGAGATATGATGAATGATTTTCTGGCATTGAGAGAACAGATTGTTAAGGAGCACAAAGACGGTCTCAAGAGAAGGTACTATAATGCCACCGGAGAGGAAGCAACTGAAGAAGTGATTGAGAAGATTTTGAGAAGTGGGGAGGTCAAAGTTTTTGAAGGGAAAACAGAGCTGGTGATGGAGAATCAGGAAAGAGATGAGGCCTTGAAGGACTTAAAGAGAAGCTTGACAGAACTGCATCAAGTTTTCCTGGACATGGCGGTGTTGGTTGAAGCACAAGGTGAACAGATTAATGATATCGAAAAGAATGTGGCTGATGCTGGGATTTACATTAGTGGCGGAAACAAGGAACTTCTTCATGCCAAGCAGTTGAAAAAGAAAAGAGGAAAGTGGATGGAAGGGTGCTCTAGCGGAGTTTTGAGCTTACTAGAACTATTGGTTGGTTCATTCGGTACAGCTGATATGGCAGTATCTCTCGTGGACTGGGGAAGTGTTCCAAACTCCCAAGTCTTTCATGCTGGTAACAATAACCTCTCAGGACTACTTTCAAACGATATTTATAATGCAACAGCACTTCAAGAAATTTCAATGGCTCACAATTCGCTTTATGGAGTAGTTGATGAGAGAATTGTCAACCTCACAAACCTCATAATCCTTGACCTCAACTCTAACCAACCGAGTGGCAACATTCCTAGTAATACTGGGACGCCCTCCAGTTTAAAATTTATGATCCTTCATACCAACAACCTAGCAGGTGTTGTAAAACGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

400

Amino Acids

44.59

Weight (kDa)

5.35

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 39 - 196 5.2e-45 Syntaxin
SNARE PF05739 238 - 275 2.8e-06 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35190 AT4G35190
malus_domestica MD05G1130400.v1.1 MD10G1133300.v1.1
prunus_persica Prupe.8G174600_v2.0.a1
pyrus_communis pycom05g12710
rosa_chinensis RchiOBHm_Chr6g0279791
rosa_laevigata RLG00000013119
rosa_multiflora Rmu_sc0002340.1_g000021
rosa_roxburghii Rroxscaffold_7G00188650
rosa_rugosa Rorug06G0128200 Rorug06G0128300
rosa_samantha Rh6AG236400 Rh6BG240500 Rh6CG242800 Rh6DG234200
rosa_wichuraiana Rw6G020600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 990
AarI CACCTGC 1 cut(s) 1172
Acc36I ACCTGC 2 cut(s) 192, 1172
AciI CCGC 3 cut(s) 689, 768, 840
AclWI GGATC 1 cut(s) 1153
AcsI RAATTY 3 cut(s) 131, 1011, 1151
AcuI CTGAAG 3 cut(s) 132, 182, 558
AcyI GRCGYC 1 cut(s) 1136
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 3 cut(s) 368, 509, 883
AfiI CCNNNNNNNGG 2 cut(s) 358, 685
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 9 cut(s) 71, 140, 587, 637, 701, 802, 978, 1007, 1017
AjnI CCWGG 1 cut(s) 678
AjuI GAANNNNNNNTTGG 2 cut(s) 852, 884
AluBI AGCT 6 cut(s) 299, 430, 601, 654, 852, 887
AluI AGCT 6 cut(s) 299, 430, 601, 654, 852, 887
Alw21I GWGCWC 2 cut(s) 486, 836
Alw26I GTCTC 2 cut(s) 252, 500
AlwI GGATC 1 cut(s) 1153
AlwNI CAGNNNCTG 2 cut(s) 80, 205
AoxI GGCC 2 cut(s) 54, 630
ApoI RAATTY 3 cut(s) 131, 1011, 1151
AseI ATTAAT 1 cut(s) 720
Asp700I GAANNNNTTC 1 cut(s) 783
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 616, 722
AvaII GGWCC 1 cut(s) 202
BauI CACGAG 1 cut(s) 905
Bbv12I GWGCWC 2 cut(s) 486, 836
BccI CCATC 2 cut(s) 601, 817
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 1 cut(s) 680
BclI TGATCA 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 252, 500
BfaI CTAG 4 cut(s) 837, 857, 1122, 1178
BfrI CTTAAG 1 cut(s) 44
BfuAI ACCTGC 2 cut(s) 192, 1172
Bme1390I CCNGG 1 cut(s) 680
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 680
BmrI ACTGGG 2 cut(s) 922, 1140
BmsI GCATC 2 cut(s) 676, 736
BmuI ACTGGG 2 cut(s) 922, 1140
BpmI CTGGAG 1 cut(s) 1126
BpuEI CTTGAG 2 cut(s) 122, 482
BsaBI GATNNNNATC 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 1136
BsaI GGTCTC 2 cut(s) 252, 500
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 685
Bse1I ACTGG 4 cut(s) 415, 917, 1135, 1143
Bse8I GATNNNNATC 1 cut(s) 322
BseBI CCWGG 1 cut(s) 680
BseGI GGATG 1 cut(s) 828
BseJI GATNNNNATC 1 cut(s) 322
BseLI CCNNNNNNNGG 2 cut(s) 358, 685
BseMII CTCAG 7 cut(s) 43, 245, 259, 281, 410, 422, 978
BseNI ACTGG 4 cut(s) 415, 917, 1135, 1143
BseYI CCCAGC 2 cut(s) 362, 749
BshFI GGCC 2 cut(s) 56, 632
BsiHKAI GWGCWC 2 cut(s) 486, 836
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 1 cut(s) 1147
BslI CCNNNNNNNGG 2 cut(s) 358, 685
BsmAI GTCTC 2 cut(s) 252, 500
BsmFI GGGAC 1 cut(s) 1147
BsnI GGCC 2 cut(s) 56, 632
Bso31I GGTCTC 2 cut(s) 252, 500
Bsp1286I GDGCHC 2 cut(s) 486, 836
Bsp143I GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
BspACI CCGC 3 cut(s) 689, 768, 840
BspANI GGCC 2 cut(s) 56, 632
BspCNI CTCAG 7 cut(s) 42, 246, 258, 282, 411, 423, 977
BspMI ACCTGC 2 cut(s) 192, 1172
BspPI GGATC 1 cut(s) 1153
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 2 cut(s) 252, 500
BsrI ACTGG 4 cut(s) 415, 917, 1135, 1143
BssMI GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
BssNI GRCGYC 1 cut(s) 1136
BssSI CACGAG 1 cut(s) 905
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 2 cut(s) 233, 494
Bst6I CTCTTC 1 cut(s) 165
BstACI GRCGYC 1 cut(s) 1136
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 8 cut(s) 29, 245, 254, 290, 300, 419, 431, 964
BstF5I GGATG 1 cut(s) 828
BstKTI GATC 6 cut(s) 10, 43, 196, 334, 391, 1161
BstMAI GTCTC 2 cut(s) 252, 500
BstMBI GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
BstMWI GCNNNNNNNGC 2 cut(s) 158, 893
BstNI CCWGG 1 cut(s) 680
BstSCI CCNGG 1 cut(s) 678
BstXI CCANNNNNNTGG 1 cut(s) 1109
BsuRI GGCC 2 cut(s) 56, 632
BtsCI GGATG 1 cut(s) 828
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 2 cut(s) 192, 1172
Cac8I GCNNGC 1 cut(s) 108
CaiI CAGNNNCTG 2 cut(s) 80, 205
Cfr13I GGNCC 1 cut(s) 202
CseI GACGC 1 cut(s) 1144
Csp6I GTAC 3 cut(s) 367, 508, 882
CviAII CATG 6 cut(s) 58, 166, 277, 685, 788, 944
CviQI GTAC 3 cut(s) 367, 508, 882
DdeI CTNAG 8 cut(s) 29, 245, 254, 290, 300, 419, 431, 964
DpnI GATC 6 cut(s) 9, 42, 195, 333, 390, 1160
DpnII GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
DraI TTTAAA 1 cut(s) 1149
DraIII CACNNNGTG 1 cut(s) 710
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
EciI GGCGGA 1 cut(s) 783
Eco147I AGGCCT 1 cut(s) 632
Eco31I GGTCTC 2 cut(s) 252, 500
Eco32I GATATC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 202
Eco57I CTGAAG 3 cut(s) 132, 182, 558
EcoO109I RGGNCCY 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 678
EcoRV GATATC 1 cut(s) 727
FaeI CATG 6 cut(s) 61, 169, 280, 688, 791, 947
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 1 cut(s) 1147
FatI CATG 6 cut(s) 57, 165, 276, 684, 787, 943
FbaI TGATCA 1 cut(s) 331
FokI GGATG 1 cut(s) 835
FspBI CTAG 4 cut(s) 837, 857, 1122, 1178
GsaI CCCAGC 2 cut(s) 366, 753
GsuI CTGGAG 1 cut(s) 1126
HaeIII GGCC 2 cut(s) 56, 632
HapII CCGG 1 cut(s) 522
HgaI GACGC 1 cut(s) 1144
Hin1I GRCGYC 1 cut(s) 1136
Hin1II CATG 6 cut(s) 61, 169, 280, 688, 791, 947
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HindIII AAGCTT 1 cut(s) 652
HinfI GANTC 5 cut(s) 121, 264, 323, 423, 613
HpaII CCGG 1 cut(s) 522
HphI GGTGA 2 cut(s) 616, 722
Hpy166II GTNNAC 5 cut(s) 225, 416, 713, 910, 1063
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 6 cut(s) 191, 200, 499, 617, 966, 1007
Hpy8I GTNNAC 5 cut(s) 225, 416, 713, 910, 1063
HpyAV CCTTC 6 cut(s) 368, 498, 581, 631, 821, 1172
HpyCH4III ACNGT 2 cut(s) 233, 494
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 2 cut(s) 667, 995
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 893
HpyF3I CTNAG 8 cut(s) 29, 245, 254, 290, 300, 419, 431, 964
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 1 cut(s) 1136
Hsp92II CATG 6 cut(s) 61, 169, 280, 688, 791, 947
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
LmnI GCTCC 1 cut(s) 481
LweI GCATC 2 cut(s) 676, 736
MaeI CTAG 4 cut(s) 837, 857, 1122, 1178
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 4 cut(s) 32, 145, 406, 950
MalI GATC 6 cut(s) 9, 42, 195, 333, 390, 1160
MboI GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
MboII GAAGA 8 cut(s) 125, 152, 182, 188, 224, 551, 565, 776
MhlI GDGCHC 2 cut(s) 486, 836
MluCI AATT 5 cut(s) 131, 1011, 1027, 1056, 1151
MroXI GAANNNNTTC 1 cut(s) 783
MseI TTAA 6 cut(s) 45, 309, 477, 644, 720, 1148
MslI CAYNNNNRTG 1 cut(s) 689
MspA1I CMGCKG 1 cut(s) 887
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 1 cut(s) 680
MvaI CCWGG 1 cut(s) 680
MwoI GCNNNNNNNGC 2 cut(s) 158, 893
NdeII GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
NlaIII CATG 6 cut(s) 61, 169, 280, 688, 791, 947
NmuCI GTSAC 2 cut(s) 145, 406
PaqCI CACCTGC 1 cut(s) 1172
PceI AGGCCT 1 cut(s) 632
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 5 cut(s) 121, 264, 323, 423, 613
PflFI GACNNNGTC 1 cut(s) 19
PfoI TCCNGGA 1 cut(s) 678
PpuMI RGGWCCY 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 720
PsiI TTATAA 1 cut(s) 990
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 678
PspFI CCCAGC 2 cut(s) 362, 749
PspGI CCWGG 1 cut(s) 678
PspPI GGNCC 1 cut(s) 202
PspPPI RGGWCCY 1 cut(s) 202
PstNI CAGNNNCTG 2 cut(s) 80, 205
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 3 cut(s) 368, 509, 883
RsaNI GTAC 3 cut(s) 367, 508, 882
RseI CAYNNNNRTG 1 cut(s) 689
SaqAI TTAA 6 cut(s) 45, 309, 477, 644, 720, 1148
Sau3AI GATC 6 cut(s) 7, 40, 193, 331, 388, 1158
Sau96I GGNCC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 2 cut(s) 486, 836
SfaNI GCATC 2 cut(s) 676, 736
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 3 cut(s) 44, 101, 497
SmoI CTYRAG 3 cut(s) 44, 101, 497
Sse9I AATT 5 cut(s) 131, 1011, 1027, 1056, 1151
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 3 cut(s) 689, 768, 840
SspI AATATT 1 cut(s) 343
SspMI CTAG 4 cut(s) 837, 857, 1122, 1178
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 1 cut(s) 678
TaaI ACNGT 2 cut(s) 233, 494
TaiI ACGT 1 cut(s) 39
TaqI TCGA 2 cut(s) 391, 729
TaqII GACCGA 1 cut(s) 276
TasI AATT 5 cut(s) 131, 1011, 1027, 1056, 1151
TfiI GAWTC 5 cut(s) 121, 264, 323, 423, 613
Tru1I TTAA 6 cut(s) 45, 309, 477, 644, 720, 1148
Tru9I TTAA 6 cut(s) 45, 309, 477, 644, 720, 1148
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 145, 406
Tsp45I GTSAC 2 cut(s) 145, 406
TspDTI ATGAA 8 cut(s) 17, 74, 225, 458, 776, 864, 932, 1154
TspRI CASTG 1 cut(s) 238
Tth111I GACNNNGTC 1 cut(s) 19
Vha464I CTTAAG 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 202
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 3 cut(s) 131, 1011, 1151
XmnI GAANNNNTTC 1 cut(s) 783
XspI CTAG 4 cut(s) 837, 857, 1122, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.