AT4G36180
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015871
Physical Location & Seq
Reverse (-)
17119973 .. 17123855
3883 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G36180.1

Sequence Viewer

Length: 3411 bp
ATGGCCATGGATATATCTCTGTTCTTCATTTTCCTGGTCATTTACGCGCCGTTAGTCTCATACGCCGATGAATCTCAAGCCGAGATTGATGCTCTCACGGCGTTTAAACTCAACCTCCATGACCCTCTCGGTGCTTTAACTTCATGGGATCCTTCAACACCAGCAGCTCCATGTGACTGGCGTGGCGTTGGCTGTACTAACCACCGTGTCACTGAGATCCGTCTTCCTCGTCTCCAACTCTCCGGACGAATCTCCGATCGTATCTCCGGTCTACGCATGCTACGGAAGCTCAGTCTCCGGTCTAACTCTTTCAACGGTACTATCCCTACTTCACTCGCTTACTGCACGCGCCTACTCTCCGTCTTTCTTCAGTACAACTCACTCTCCGGTAAACTACCTCCGGCGATGAGGAATCTCACCTCGCTTGAGGTCTTTAACGTCGCTGGAAACCGTCTCTCCGGTGAGATCCCCGTTGGTTTACCATCGAGTCTCCAGTTCCTTGACATTTCGTCGAACACCTTCTCTGGTCAGATACCGAGTGGACTCGCTAACTTGACTCAGCTTCAGCTTCTTAATCTCTCGTATAATCAGTTAACCGGCGAGATTCCGGCGAGTCTAGGTAACCTCCAAAGTCTTCAGTACTTATGGCTTGATTTCAATCTCTTGCAAGGAACATTACCGTCTGCTATCTCAAACTGCTCCTCCTTAGTTCACTTGAGCGCGTCGGAGAACGAAATCGGCGGCGTGATTCCCGCCGCGTACGGTGCTCTTCCGAAACTGGAGGTTCTCTCGCTCTCCAATAACAATTTTTCCGGTACTGTGCCGTTTTCTCTCTTCTGCAACACATCGCTTACGATTGTTCAGTTAGGGTTCAATGCTTTCTCCGATATCGTACGGCCTGAGACGACGGCGAACTGTCGTACCGGTTTACAGGTCTTGGATCTCCAGGAAAATCGAATCTCCGGTCGTTTTCCGTTGTGGTTGACTAATATTTTGTCACTAAAGAATCTTGATGTTTCTGGAAACTTGTTTTCCGGTGAGATTCCGCCGGATATTGGTAACTTGAAGAGATTAGAGGAACTGAAATTGGCTAATAACTCTCTTACTGGTGAGATTCCTGTTGAGATTAAGCAATGTGGTTCGTTAGATGTTTTAGATTTCGAAGGAAATAGTTTAAAAGGTCAGATTCCAGAATTTCTTGGCTACATGAAGGCTTTAAAGGTTTTATCTTTAGGCAGAAACAGCTTCTCAGGTTATGTTCCTTCGTCTATGGTGAATCTGCAGCAGCTTGAGAGGCTAAACTTAGGTGAAAACAACTTAAATGGGAGTTTTCCGGTGGAGTTGATGGCGTTGACAAGTCTGTCTGAGCTGGATCTGAGTGGAAATCGGTTTTCCGGGGCAGTTCCGGTTAGTATCAGCAACTTAAGTAACCTTAGTTTTTTGAATTTGAGTGGGAATGGATTTTCTGGTGAGATACCAGCTAGTGTAGGCAATCTGTTTAAGTTAACAGCTCTTGATTTGAGCAAGCAGAATATGTCTGGTGAGGTTCCGGTTGAGCTCTCTGGTTTGCCCAACGTGCAAGTGATTGCATTGCAGGGAAACAACTTCTCTGGGGTTGTACCTGAAGGGTTTAGTAGCTTGGTGAGTTTAAGGTATGTGAACCTTAGCTCGAACTCATTCTCCGGGGAAATACCGCAGACTTTTGGGTTTCTTCGGTTGTTGGTTTCTTTGTCACTGTCAGATAATCACATCTCTGGGTCTATTCCACCTGAAATTGGAAACTGCTCAGCTCTTGAAGTTCTTGAGTTAAGATCTAACCGGCTAATGGGTCATATTCCAGCTGATCTCTCTCGTCTTCCGCGTTTGAAGGTGCTTGACTTGGGTCAGAACAACTTGTCAGGCGAAATCCCACCAGAAATCTCTCAGAGCTCGTCTCTCAACTCATTGTCACTTGACCACAATCATCTTTCAGGAGTCATACCTGGATCTTTTTCTGGTTTATCAAACTTGACAAAGATGGATCTTTCTGTTAATAACTTAACCGGGGAGATTCCGGCTAGCCTCGCCCTTATTTCTAGTAACCTGGTATACTTCAACGTCTCAAGCAACAACCTTAAAGGAGAGATTCCAGCTTCTTTAGGCTCAAGAATCAACAACACATCAGAATTTTCAGGCAACACAGAGCTCTGCGGGAAGCCATTGAACAGAAGATGTGAAAGCAGCACAGCGGAGGGGAAGAAGAAGAAAAGGAAAATGATTCTGATGATAGTTATGGCTGCAATTGGTGCATTCCTCTTGTCACTCTTCTGCTGCTTCTACGTTTACACACTCTTGAAATGGAGGAAGAAGCTGAAACAACAGTCTACAACGGGAGAGAAGAAACGCAGTCCTGGTAGAACAAGCGCAGGAAGTAGAGTTCGTAGCAGCACAAGCAGATCAAGCACAGAAAACGGAGAACCAAAGCTAGTGATGTTCAATAACAAGATCACTCTAGCTGAAACAATTGAAGCAACACGACAGTTCGATGAAGAAAACGTTCTTAGCAGAACCCGATATGGATTGCTATTTAAAGCCAATTACAATGACGGAATGGTTCTCTCTATCCGCCGCTTACCCAATGGATCACTCTTAAATGAGAACTTGTTCAAAAAGGAAGCCGAAGTTCTTGGTAAAGTCAAGCACCGAAACATCACAGTCCTTAGAGGTTACTACGCAGGTCCACCAGATTTAAGGCTTTTGGTATATGACTACATGCCCAATGGGAACTTATCTACCCTGCTCCAAGAAGCTTCCCACCAAGATGGCCATGTCCTAAACTGGCCAATGCGCCACCTCATAGCTCTCGGGATCGCGCGCGGACTCGGATTCCTCCATCAATCCAACATGGTTCATGGTGATATCAAGCCACAGAATGTACTCTTCGACGCTGACTTTGAAGCCCATATATCTGATTTCGGGCTTGACCGTCTCACAATTCGATCCCCATCAAGATCAGCCGTCACAGCAAACACCATTGGGACACTAGGATATGTCTCTCCAGAAGCCACATTGTCCGGGGAAATCACAAGAGAATCTGACATCTACAGCTTCGGCATTGTCCTACTCGAAATCCTAACTGGAAAAAGACCAGTAATGTTCACACAAGACGAGGATATAGTGAAATGGGTAAAGAAACAGCTTCAGAGAGGTCAAGTTACAGAGCTACTAGAACCAGGACTACTCGAACTTGACCCGGAATCATCAGAATGGGAAGAGTTCTTATTAGGAATCAAAGTCGGACTTCTTTGTACTGCAACAGATCCTCTAGACCGTCCAACAATGTCCGATGTTGTTTTCATGCTTGAAGGCTGTCGTGTTGGTCCTGACGTTCCTTCCTCCGCCGACCCAACCTCTCAGCCTTCACCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1136

Amino Acids

123.66

Weight (kDa)

5.99

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 66 1.8e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 163 - 222 7.5e-06 Leucine rich repeat
LRR_14 PF23598 179 - 267 4.9e-08 Leucine-rich repeat region
LRR_8 PF13855 211 - 270 2.6e-07 Leucine rich repeat
LRR_14 PF23598 308 - 433 4.4e-09 Leucine-rich repeat region
LRR_8 PF13855 405 - 464 1.3e-09 Leucine rich repeat
LRR_8 PF13855 524 - 584 2.5e-09 Leucine rich repeat
LRR_8 PF13855 574 - 632 2.8e-06 Leucine rich repeat
LRR_8 PF13855 645 - 705 3.6e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 849 - 1061 9.2e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 880 - 1110 3.3e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36180
fragaria_vesca FvH4_5g31360
prunus_persica Prupe.7G166100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0226621
rosa_laevigata RLG00000001713
rosa_multiflora Rmu_co8455755.1_g000001 Rmu_sc0002043.1_g000006 Rmu_sc0004294.1_g000002
rosa_roxburghii Rroxscaffold_3G00232650
rosa_rugosa Rorug07G0238700
rosa_samantha Rh7AG386200 Rh7BG372900 Rh7CG405500 Rh7DG384900
rosa_wichuraiana Rw7G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1360
Acc36I ACCTGC 1 cut(s) 2705
AccI GTMKAC 3 cut(s) 271, 2088, 2363
AccII CGCG 8 cut(s) 47, 349, 722, 758, 1861, 2852, 2854, 2856
AccIII TCCGGA 1 cut(s) 242
AclI AACGTT 1 cut(s) 2535
AcoI YGGCCR 3 cut(s) 3, 2803, 2819
AcsI RAATTY 3 cut(s) 1193, 1444, 2165
AcuI CTGAAG 5 cut(s) 353, 548, 620, 1644, 3164
AfiI CCNNNNNNNGG 3 cut(s) 1055, 1775, 1825
AflII CTTAAG 1 cut(s) 1423
AgeI ACCGGT 1 cut(s) 923
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 6 cut(s) 33, 945, 1981, 2082, 2389, 3211
AloI GAACNNNNNNTCC 4 cut(s) 1664, 1696, 2400, 2432
Alw21I GWGCWC 4 cut(s) 769, 1560, 1931, 2187
Ama87I CYCGRG 1 cut(s) 2843
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 5 cut(s) 3, 896, 2803, 2819, 3405
ApeKI GCWGC 7 cut(s) 164, 1282, 1285, 2220, 2276, 2310, 2424
ApoI RAATTY 3 cut(s) 1193, 1444, 2165
ArsI GACNNNNNNTTYG 6 cut(s) 949, 976, 981, 1008, 2915, 2947
AsiGI ACCGGT 1 cut(s) 923
Asp700I GAANNNNTTC 4 cut(s) 518, 1676, 2535, 2642
AspLEI GCGC 7 cut(s) 49, 351, 722, 2405, 2829, 2854, 2856
AspS9I GGNCC 2 cut(s) 2717, 3359
AsuC2I CCSGG 5 cut(s) 1396, 1684, 2044, 3055, 3233
AsuII TTCGAA 1 cut(s) 1161
AsuNHI GCTAGC 1 cut(s) 2057
AvaI CYCGRG 1 cut(s) 2843
AvaII GGWCC 2 cut(s) 2717, 3359
BaeI ACNNNNGTAYC 2 cut(s) 904, 937
BalI TGGCCA 3 cut(s) 5, 2805, 2821
BamHI GGATCC 1 cut(s) 148
BanII GRGCYC 3 cut(s) 1560, 1931, 2187
BbsI GAAGAC 3 cut(s) 215, 626, 1847
Bbv12I GWGCWC 4 cut(s) 769, 1560, 1931, 2187
BbvI GCAGC 7 cut(s) 176, 1294, 1297, 2232, 2263, 2297, 2436
BccI CCATC 6 cut(s) 490, 1339, 2011, 2795, 2880, 2992
BceAI ACGGC 6 cut(s) 34, 114, 808, 911, 924, 2981
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 6 cut(s) 35, 947, 1983, 2084, 2391, 3213
BcnI CCSGG 5 cut(s) 1396, 1684, 2044, 3055, 3233
BfaI CTAG 9 cut(s) 617, 1482, 2058, 2076, 2465, 2492, 3023, 3206, 3305
BfmI CTRYAG 2 cut(s) 1280, 3082
BfrI CTTAAG 1 cut(s) 1423
BfuAI ACCTGC 1 cut(s) 2705
BglII AGATCT 1 cut(s) 1811
BlpI GCTNAGC 1 cut(s) 1786
BmcAI AGTACT 1 cut(s) 641
Bme18I GGWCC 2 cut(s) 2717, 3359
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 2843
BmgT120I GGNCC 2 cut(s) 2717, 3359
BmiI GGNNCC 2 cut(s) 150, 1548
BmsI GCATC 1 cut(s) 79
BmtI GCTAGC 1 cut(s) 2061
BoxI GACNNNNGTC 1 cut(s) 1881
BpiI GAAGAC 3 cut(s) 215, 626, 1847
BplI GAGNNNNNCTC 4 cut(s) 773, 805, 1918, 1950
BpmI CTGGAG 4 cut(s) 476, 800, 929, 3021
Bpu10I CCTNAGC 1 cut(s) 1664
Bpu1102I GCTNAGC 1 cut(s) 1786
Bpu14I TTCGAA 1 cut(s) 1161
BpuEI CTTGAG 7 cut(s) 60, 446, 736, 1310, 1823, 2086, 2128
BpuMI CCSGG 5 cut(s) 1396, 1684, 2044, 3055, 3233
BsaBI GATNNNNATC 2 cut(s) 657, 2983
BsaJI CCNNGG 5 cut(s) 6, 1395, 1683, 2043, 3054
Bsc4I CCNNNNNNNGG 3 cut(s) 1055, 1775, 1825
Bse118I RCCGGY 4 cut(s) 596, 923, 1818, 3403
Bse1I ACTGG 7 cut(s) 182, 493, 783, 1111, 2822, 3121, 3128
Bse3DI GCAATG 2 cut(s) 1139, 1589
Bse8I GATNNNNATC 2 cut(s) 657, 2983
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 6 cut(s) 35, 947, 1983, 2084, 2391, 3213
BseDI CCNNGG 5 cut(s) 6, 1395, 1683, 2043, 3054
BseJI GATNNNNATC 2 cut(s) 657, 2983
BseLI CCNNNNNNNGG 3 cut(s) 1055, 1775, 1825
BseMI GCAATG 2 cut(s) 1139, 1589
BseNI ACTGG 7 cut(s) 182, 493, 783, 1111, 2822, 3121, 3128
BsePI GCGCGC 1 cut(s) 2852
BseRI GAGGAG 1 cut(s) 691
BseXI GCAGC 7 cut(s) 176, 1294, 1297, 2232, 2263, 2297, 2436
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 8 cut(s) 47, 349, 722, 758, 1861, 2852, 2854, 2856
Bsh1285I CGRYCG 2 cut(s) 259, 967
BshFI GGCC 5 cut(s) 5, 898, 2805, 2821, 3407
BshTI ACCGGT 1 cut(s) 923
BsiEI CGRYCG 2 cut(s) 259, 967
BsiHKAI GWGCWC 4 cut(s) 769, 1560, 1931, 2187
BsiHKCI CYCGRG 1 cut(s) 2843
BsiWI CGTACG 2 cut(s) 759, 892
BslFI GGGAC 1 cut(s) 3031
BslI CCNNNNNNNGG 3 cut(s) 1055, 1775, 1825
BsmBI CGTCTC 6 cut(s) 236, 458, 896, 1938, 2104, 2972
BsmFI GGGAC 1 cut(s) 3031
BsmI GAATGC 1 cut(s) 2288
BsnI GGCC 5 cut(s) 5, 898, 2805, 2821, 3407
BsoBI CYCGRG 1 cut(s) 2843
Bsp119I TTCGAA 1 cut(s) 1161
Bsp1286I GDGCHC 4 cut(s) 769, 1560, 1931, 2187
Bsp13I TCCGGA 1 cut(s) 242
Bsp1720I GCTNAGC 1 cut(s) 1786
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 5 cut(s) 5, 898, 2805, 2821, 3407
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 8 cut(s) 47, 349, 722, 758, 1861, 2852, 2854, 2856
BspLI GGNNCC 2 cut(s) 150, 1548
BspMAI CTGCAG 1 cut(s) 1284
BspMI ACCTGC 1 cut(s) 2705
BspOI GCTAGC 1 cut(s) 2061
BspQI GCTCTTC 1 cut(s) 774
BspT104I TTCGAA 1 cut(s) 1161
BspTI CTTAAG 1 cut(s) 1423
BsrDI GCAATG 2 cut(s) 1139, 1589
BsrFI RCCGGY 4 cut(s) 596, 923, 1818, 3403
BsrI ACTGG 7 cut(s) 182, 493, 783, 1111, 2822, 3121, 3128
BssAI RCCGGY 4 cut(s) 596, 923, 1818, 3403
BssECI CCNNGG 5 cut(s) 6, 1395, 1683, 2043, 3054
BssHII GCGCGC 1 cut(s) 2852
BssNAI GTATAC 1 cut(s) 2089
BssT1I CCWWGG 1 cut(s) 6
Bst1107I GTATAC 1 cut(s) 2089
Bst2UI CCWGG 6 cut(s) 35, 947, 1983, 2084, 2391, 3213
Bst6I CTCTTC 6 cut(s) 774, 839, 1061, 2309, 2924, 3246
BstAFI CTTAAG 1 cut(s) 1423
BstAPI GCANNNNNTGC 1 cut(s) 2285
BstBI TTCGAA 1 cut(s) 1161
BstC8I GCNNGC 5 cut(s) 278, 347, 1526, 2059, 2854
BstDSI CCRYGG 1 cut(s) 6
BstEII GGTNACC 1 cut(s) 620
BstFNI CGCG 8 cut(s) 47, 349, 722, 758, 1861, 2852, 2854, 2856
BstHHI GCGC 7 cut(s) 49, 351, 722, 2405, 2829, 2854, 2856
BstMCI CGRYCG 2 cut(s) 259, 967
BstNI CCWGG 6 cut(s) 35, 947, 1983, 2084, 2391, 3213
BstNSI RCATGY 2 cut(s) 280, 2755
BstPAI GACNNNNGTC 1 cut(s) 1881
BstPI GGTNACC 1 cut(s) 620
BstSFI CTRYAG 2 cut(s) 1280, 3082
BstUI CGCG 8 cut(s) 47, 349, 722, 758, 1861, 2852, 2854, 2856
BstV1I GCAGC 7 cut(s) 176, 1294, 1297, 2232, 2263, 2297, 2436
BstV2I GAAGAC 3 cut(s) 215, 626, 1847
BstX2I RGATCY 9 cut(s) 148, 216, 465, 940, 1372, 1811, 1985, 2020, 3298
BstXI CCANNNNNNTGG 2 cut(s) 177, 2801
BstYI RGATCY 9 cut(s) 148, 216, 465, 940, 1372, 1811, 1985, 2020, 3298
BstZ17I GTATAC 1 cut(s) 2089
BsuRI GGCC 5 cut(s) 5, 898, 2805, 2821, 3407
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 2 cut(s) 419, 831
BtsIMutI CAGTG 2 cut(s) 210, 1733
BveI ACCTGC 1 cut(s) 2705
Cac8I GCNNGC 5 cut(s) 278, 347, 1526, 2059, 2854
CfoI GCGC 7 cut(s) 49, 351, 722, 2405, 2829, 2854, 2856
Cfr10I RCCGGY 4 cut(s) 596, 923, 1818, 3403
Cfr13I GGNCC 2 cut(s) 2717, 3359
CseI GACGC 2 cut(s) 711, 2933
CsiI ACCWGGT 1 cut(s) 2082
CspAI ACCGGT 1 cut(s) 923
DraI TTTAAA 4 cut(s) 106, 1176, 1218, 2569
DrdI GACNNNNNNGTC 1 cut(s) 1360
DriI GACNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 1360
EaeI YGGCCR 3 cut(s) 3, 2803, 2819
Eam1104I CTCTTC 6 cut(s) 774, 839, 1061, 2309, 2924, 3246
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 6 cut(s) 774, 839, 1061, 2309, 2924, 3246
EciI GGCGGA 3 cut(s) 1035, 2594, 3367
Ecl136II GAGCTC 3 cut(s) 1558, 1929, 2185
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 3 cut(s) 1560, 1931, 2187
Eco32I GATATC 2 cut(s) 889, 2899
Eco47I GGWCC 2 cut(s) 2717, 3359
Eco53kI GAGCTC 3 cut(s) 1558, 1929, 2185
Eco57I CTGAAG 5 cut(s) 353, 548, 620, 1644, 3164
Eco88I CYCGRG 1 cut(s) 2843
Eco91I GGTNACC 1 cut(s) 620
EcoICRI GAGCTC 3 cut(s) 1558, 1929, 2185
EcoO65I GGTNACC 1 cut(s) 620
EcoRII CCWGG 6 cut(s) 33, 945, 1981, 2082, 2389, 3211
EcoRV GATATC 2 cut(s) 889, 2899
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 1560, 1931, 2187
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 6 cut(s) 236, 458, 896, 1938, 2104, 2972
FalI AAGNNNNNCTT 2 cut(s) 3264, 3296
FaqI GGGAC 1 cut(s) 3031
FauI CCCGC 2 cut(s) 760, 2183
FblI GTMKAC 3 cut(s) 271, 2088, 2363
FriOI GRGCYC 3 cut(s) 1560, 1931, 2187
FspBI CTAG 9 cut(s) 617, 1482, 2058, 2076, 2465, 2492, 3023, 3206, 3305
GlaI GCGC 7 cut(s) 48, 350, 721, 2404, 2828, 2853, 2855
GsuI CTGGAG 4 cut(s) 476, 800, 929, 3021
HaeIII GGCC 5 cut(s) 5, 898, 2805, 2821, 3407
HgaI GACGC 2 cut(s) 711, 2933
HhaI GCGC 7 cut(s) 49, 351, 722, 2405, 2829, 2854, 2856
Hin6I GCGC 7 cut(s) 47, 349, 720, 2403, 2827, 2852, 2854
HinP1I GCGC 7 cut(s) 47, 349, 720, 2403, 2827, 2852, 2854
HincII GTYRAC 4 cut(s) 594, 984, 1353, 1506
HindII GTYRAC 4 cut(s) 594, 984, 1353, 1506
HindIII AAGCTT 1 cut(s) 2787
HpaI GTTAAC 2 cut(s) 594, 1506
Hpy99I CGWCG 5 cut(s) 443, 514, 727, 910, 2927
HpyCH4IV ACGT 6 cut(s) 438, 1575, 2097, 2319, 2535, 3366
HpySE526I ACGT 6 cut(s) 438, 1575, 2097, 2319, 2535, 3366
HspAI GCGC 7 cut(s) 47, 349, 720, 2403, 2827, 2852, 2854
Kpn2I TCCGGA 1 cut(s) 242
KspAI GTTAAC 2 cut(s) 594, 1506
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 3 cut(s) 172, 704, 2784
Lsp1109I GCAGC 7 cut(s) 176, 1294, 1297, 2232, 2263, 2297, 2436
LweI GCATC 1 cut(s) 79
MabI ACCWGGT 1 cut(s) 2082
MaeI CTAG 9 cut(s) 617, 1482, 2058, 2076, 2465, 2492, 3023, 3206, 3305
MaeII ACGT 6 cut(s) 438, 1575, 2097, 2319, 2535, 3366
MauBI CGCGCGCG 1 cut(s) 2852
MfeI CAATTG 2 cut(s) 2280, 2502
MflI RGATCY 9 cut(s) 148, 216, 465, 940, 1372, 1811, 1985, 2020, 3298
MhlI GDGCHC 4 cut(s) 769, 1560, 1931, 2187
MlsI TGGCCA 3 cut(s) 5, 2805, 2821
MluNI TGGCCA 3 cut(s) 5, 2805, 2821
MlyI GAGTC 6 cut(s) 496, 537, 550, 622, 1983, 2853
MmeI TCCRAC 5 cut(s) 259, 705, 2904, 3256, 3338
Mox20I TGGCCA 3 cut(s) 5, 2805, 2821
MroI TCCGGA 1 cut(s) 242
MroXI GAANNNNTTC 4 cut(s) 518, 1676, 2535, 2642
MscI TGGCCA 3 cut(s) 5, 2805, 2821
MslI CAYNNNNRTG 2 cut(s) 2799, 3244
Msp20I TGGCCA 3 cut(s) 5, 2805, 2821
MspA1I CMGCKG 2 cut(s) 1841, 2228
MspCI CTTAAG 1 cut(s) 1423
MssI GTTTAAAC 1 cut(s) 106
MunI CAATTG 2 cut(s) 2280, 2502
Mva1269I GAATGC 1 cut(s) 2288
MvaI CCWGG 6 cut(s) 35, 947, 1983, 2084, 2391, 3213
MvnI CGCG 8 cut(s) 47, 349, 722, 758, 1861, 2852, 2854, 2856
NciI CCSGG 5 cut(s) 1396, 1684, 2044, 3055, 3233
NcoI CCATGG 1 cut(s) 6
NheI GCTAGC 1 cut(s) 2057
NlaIV GGNNCC 2 cut(s) 150, 1548
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 7 cut(s) 173, 208, 996, 1731, 1947, 2298, 2998
NspI RCATGY 2 cut(s) 280, 2755
NspV TTCGAA 1 cut(s) 1161
PaeI GCATGC 1 cut(s) 280
PauI GCGCGC 1 cut(s) 2852
PciSI GCTCTTC 1 cut(s) 774
PcsI WCGNNNNNNNCGW 1 cut(s) 1858
PctI GAATGC 1 cut(s) 2288
PdmI GAANNNNTTC 4 cut(s) 518, 1676, 2535, 2642
Pfl23II CGTACG 2 cut(s) 759, 892
PfoI TCCNGGA 1 cut(s) 945
PinAI ACCGGT 1 cut(s) 923
Ple19I CGATCG 1 cut(s) 259
PleI GAGTC 6 cut(s) 495, 537, 550, 621, 1982, 2853
PmeI GTTTAAAC 1 cut(s) 106
PpsI GAGTC 6 cut(s) 495, 537, 550, 621, 1982, 2853
PshAI GACNNNNGTC 1 cut(s) 1881
Psp124BI GAGCTC 3 cut(s) 1560, 1931, 2187
Psp1406I AACGTT 1 cut(s) 2535
Psp6I CCWGG 6 cut(s) 33, 945, 1981, 2082, 2389, 3211
PspEI GGTNACC 1 cut(s) 620
PspGI CCWGG 6 cut(s) 33, 945, 1981, 2082, 2389, 3211
PspLI CGTACG 2 cut(s) 759, 892
PspN4I GGNNCC 2 cut(s) 150, 1548
PspPI GGNCC 2 cut(s) 2717, 3359
PsrI GAACNNNNNNTAC 2 cut(s) 3201, 3233
PstI CTGCAG 1 cut(s) 1284
PsuI RGATCY 9 cut(s) 148, 216, 465, 940, 1372, 1811, 1985, 2020, 3298
PteI GCGCGC 1 cut(s) 2852
PvuI CGATCG 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 1841
RseI CAYNNNNRTG 2 cut(s) 2799, 3244
SacI GAGCTC 3 cut(s) 1560, 1931, 2187
SapI GCTCTTC 1 cut(s) 774
Sau96I GGNCC 2 cut(s) 2717, 3359
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 6 cut(s) 496, 537, 550, 622, 1983, 2853
SduI GDGCHC 4 cut(s) 769, 1560, 1931, 2187
SexAI ACCWGGT 1 cut(s) 2082
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 2 cut(s) 1280, 3082
SfuI TTCGAA 1 cut(s) 1161
SinI GGWCC 2 cut(s) 2717, 3359
SmiMI CAYNNNNRTG 2 cut(s) 2799, 3244
SmlI CTYRAG 8 cut(s) 75, 425, 715, 1289, 1423, 1802, 2101, 2143
SmoI CTYRAG 8 cut(s) 75, 425, 715, 1289, 1423, 1802, 2101, 2143
SphI GCATGC 1 cut(s) 280
SspI AATATT 1 cut(s) 991
SspMI CTAG 9 cut(s) 617, 1482, 2058, 2076, 2465, 2492, 3023, 3206, 3305
SstI GAGCTC 3 cut(s) 1560, 1931, 2187
StyI CCWWGG 1 cut(s) 6
TaiI ACGT 6 cut(s) 441, 1578, 2100, 2322, 2538, 3369
TatI WGTACW 5 cut(s) 194, 372, 639, 2914, 3287
TauI GCSGC 3 cut(s) 744, 758, 2610
TscAI CASTG 2 cut(s) 217, 1740
TseFI GTSAC 7 cut(s) 173, 208, 996, 1731, 1947, 2298, 2998
TseI GCWGC 7 cut(s) 164, 1282, 1285, 2220, 2276, 2310, 2424
Tsp45I GTSAC 7 cut(s) 173, 208, 996, 1731, 1947, 2298, 2998
TspDTI ATGAA 7 cut(s) 16, 84, 132, 1223, 2541, 2879, 3325
TspGWI ACGGA 6 cut(s) 209, 298, 349, 963, 2466, 2601
TspRI CASTG 2 cut(s) 217, 1740
Vha464I CTTAAG 1 cut(s) 1423
VpaK11BI GGWCC 2 cut(s) 2717, 3359
XapI RAATTY 3 cut(s) 1193, 1444, 2165
XbaI TCTAGA 1 cut(s) 3304
XceI RCATGY 2 cut(s) 280, 2755
XmiI GTMKAC 3 cut(s) 271, 2088, 2363
XmnI GAANNNNTTC 4 cut(s) 518, 1676, 2535, 2642
XspI CTAG 9 cut(s) 617, 1482, 2058, 2076, 2465, 2492, 3023, 3206, 3305
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.