RLG00000001713
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015871
Physical Location & Seq
Reverse (-)
19428532 .. 19431963
3432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001713

Sequence Viewer

Length: 3432 bp
ATGAAACCCATTATGCCTGCGCTTCTTCTTTTCTTTCTTGTGCTTTCCGGCGCAACCTTCTTCTCCTGCGCCGCCCAGCGCAGCCCAGAAAGCCAAGCCGAGATCGACGCCCTCACCACCTTCAGGCTAGACCTCCAGGATCCGATTGGCGCATTGAACGGCTGGGATTCCTCCACGCCTTCCGCTCCCTGCGACTGGCGCGGCGTCTTTTGCGACAGCACCACTCGGGTCTCCGAGCTCCGCTTGCCTCGCCTCCAACTCGGCGGCCGACTCAGTGACTCGCTCGCGAAGCTCACCATGCTTGGAAAGCTCAGCCTCCGGTCGAACTCCTTCAACGGGAGTATTCCTGACTCGCTTTCCCAATGCACGCGCCTACGCGCCGTCTTCTTGCAGAACAACTCTCTCTCCGGGAAGCTGCCGCCGGAGATCGGGAACCTGACCGGGCTGCAGATTCTCAATGTCGCCGGTAACCGCCTCTCCGGCGAAATCTCCGGCGAGCTCCCTCCGAGCCTCGTGTACCTCGATCTCTCCTCGAACGATTTCGCCGGCGAGATTCCGAGGAGCATCGCGAACCTGACTCAGCTTCAGCTCATCAACCTGTCGTACAACCAGTTTTCCGGTCAGGTTCCGGCGAGCTTGGGCCAGCTCCAGCAGCTGCAGTTCCTCTGGATGGACCACAACCTCCTGGAAGGAACGCTGCCGTCGGCGATAGCCAATTGCGCGGCGCTGGTGCATTTCAGCGTCGAAGGGAATGCGCTCGGCGGCGTGATTCCGGCGGCCATCGGAGCTCTGCCGAAGCTTCAGGTGTTGTCGCTTTCACAGAATAATTTCTCTGGTATGGTCCCATCCTCGTTGTTTTGCAATGCCTCATTCAATACGGCGTCGCTTAGGATTGTCCAGCTAGGGTTTAATTCGTTCACGGATATTGTTAAGCCTGAGACAGCCACATGTTTTAGTGCTCTGGTGGTTTTGAATCTTCAGCACAATCAGATTGGCGGCGATTTTCCTTGGTGGATAATAACACAGGTACCAAACTTGACAGTGTTGGATGTTTCTAGTAACTCATTCTCCGGCGTGGTGCCGCCGGGGATTGGAAACCTCTCGAGGTTACAGGAGCTTAAGATGGCTAATAATTCATTTAGCGGTCCGATTCCGCAAGAGATTAAGCAATGCAGCTCACTAAGCGTGCTTGATCTCCAAGGAAATCGATTTTCGGGTGAAATTCCTGTGTTTTTGGGGGATTTGACAGGTTTGAAGGTGCTATCTTTAGGGGAAAATCAGTTCTCGGGTTCCATTCCTCCGAGTTTTGGTAATCTTACGAGGCTGGAGACTTTGGGATTGGGAGGGAACAACTTGACCGGAACTATGCCTGAGGAGTTGTTAATAGGGTTGGGTAATTTGACCACATTGGACCTCAGTGGGAACAGGTTTTTTGGTGAAGTTGCCATCAGCATTGGGAGTTTGAGTCAGTTAATGGTGCTGAATCTGAGTGGCAATGGCTTTTCTGGGAGGGTGCCGACTAGCTTGGGTAGTCTTTTCAGGCTTACTACTATTGATCTGAGCAAACAAAACTTCTCTGGTGAGGTGCCTTCTGAGCTTTTGGGGTTACCCAATTTACAAGTCATTGCATTGCAAGAGAACAGGTTGTCTGGAGACGTTCCTGAAGGTTTTAGTAGCTTGATGGGTCTGCATTATTTGAACCTCAGTTCAAATGCATTTTCTGGTCACATTCCGGAGAATTATGGTTTTCTTCGGTCATTGGTTGTTCTTTCATTGTCCAACAATCACATTTCAGGACCAATTCCACTGGAGCTTGGAAACTGCTCTGATCTTCAAGTTCTAGAGCTGCAAATGAATTCCTTGAGTGGCACCATTCCAGCCGATCTCTCTCGCCTATCAGTGTTAAAGGATCTTGATTTGGGTAGCAACAACTTAACAGGTAGAATCCCAGAGGAAATGTCTAAATGCTCATCATTAACTGCTTTGGTGTTGGACTCCAATCATCTCTCAGGTGGTATACCAGAATCGTTGTCTAAGCTATTGAACCTAACTACACTGGATCTCTCCAATAACAATTTGAGCGGGCAAATTCCTGGTGATCTTGCATCTATTCCTGGCCTAGTGAACTTCAATGTCTCGATGAATAACCTCAACGGTGAGATTCCAGTGACGCTTGGTTCTCGGTTCAACAATGCATCAGACTTTTCAGGAAATCAAAACTTATGTGGGCCGCCATTGGAAAAGAAGTGCAAGGATCTGGACAAGAAAGATAGAAATAAGAGATTAATTCTTTTGATTGTGATTGTTGTTAGTGGAGCTTGCCTATTAGCTTTGTGCTGCTGCTTCTACATTTTCAGCCTCTTGAGATGGCGCAAAAAGCTCAAACAGGGATCATCTGGAGAGAAGACTAGAAGCAGTGCGAGGGCAAGCTCAGGAACTAGTGAGGGCCGTGGAAGCGCTGATAATGGAGTGCCAAAGCTTGTCATGTTCAACAGCAAAATCACACTTGGCGAGACAATTGCAGCCACAAGGCAATTCGACGAAGAAAATGTGCTGAGCAGATCACCATATGGCTTGGTTTTCAAGGCTTGTTATGAGGATGGCATGGTGATGTCCATCCGTCGCCTCCCCGATGCAACACTCGATGAAAACATGTTCCGAAAAGAAGCAGAATCACTAGGCAGAGTCAAGCACCGGAATTTAACAGTTCTTCGAGGCTACTATGCCGGTCCACCTGACCTCAGGCTTCTTATCTATGACTACATGCCCAACGGTAACCTAGCCACCCTTCTGCAAGAAGCATCACACCAAGACGGCCATGTCCTCAATTGGCCAATGCGTCATCTCATTGCACTTGGAATTGCTCGTGGCTTGGCTTTTCTACACACATCTTCACTGGTACACGGCGACGTGAAGCCACAAAGCGTGCTATTCGATGCAGATTTCGAAGCCCATTTGTCTGATTTCGGCCTAGACAAACTCACATCAGCCACCAGCACTACGACTCCAACCGAAGCCTCAACCTCAACCACCGTCGGGACATTAGGCTACGTCTCTCACGAAGCAACACTAACGGGCAGAGTCACAAAGGAATCCGATGTTTACAGCTACGGGATCGTCTTGCTCGAACTCTTAACAGGAAGGAGGCCGGTGATGTTCACCGAAGACGAAGACATAGTGAAGTGGGTGAAGAAGCAGCTTCAAAAAGGCCAAGTCACGGAGCTACTAGAGCCAGGACTGCTCGAGCTTGACCCCGAGTCATCCGAGTGGGAAGAGTTTTTGCTTGGCGTGAAAGTCGGGTTGCTCTGCACGGCACCCGACCCGCTGGACCGGCCCACCATGTCCGACATTGTTTTCATGCTCGAAGGCTGCCGGGTCGGACCCGATCTCCCGTCCTCCGCCGACCCGACGTCGCAACCGTCGCCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1144

Amino Acids

123.04

Weight (kDa)

5.22

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 72 9.1e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 169 - 229 1.6e-06 Leucine rich repeat
LRR_14 PF23598 171 - 352 7.1e-10 Leucine-rich repeat region
LRR_8 PF13855 344 - 404 8.3e-08 Leucine rich repeat
LRR_14 PF23598 345 - 470 1.3e-08 Leucine-rich repeat region
LRR_14 PF23598 508 - 736 2.7e-12 Leucine-rich repeat region
LRR_8 PF13855 514 - 572 5.2e-07 Leucine rich repeat
LRR_8 PF13855 537 - 597 2.1e-07 Leucine rich repeat
LRR_8 PF13855 611 - 669 1.4e-06 Leucine rich repeat
LRR_8 PF13855 658 - 717 7.6e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 850 - 1068 3.4e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 856 - 1117 1.9e-29 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36180
fragaria_vesca FvH4_5g31360
prunus_persica Prupe.7G166100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0226621
rosa_laevigata RLG00000001713
rosa_multiflora Rmu_co8455755.1_g000001 Rmu_sc0002043.1_g000006 Rmu_sc0004294.1_g000002
rosa_roxburghii Rroxscaffold_3G00232650
rosa_rugosa Rorug07G0238700
rosa_samantha Rh7AG386200 Rh7BG372900 Rh7CG405500 Rh7DG384900
rosa_wichuraiana Rw7G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2819
AatII GACGTC 1 cut(s) 3413
Acc65I GGTACC 1 cut(s) 1027
AccB1I GGYRCC 6 cut(s) 1027, 1078, 1513, 1585, 1868, 3313
AccBSI CCGCTC 2 cut(s) 185, 2082
AccI GTMKAC 1 cut(s) 2017
AccII CGCG 6 cut(s) 201, 287, 370, 378, 569, 722
AccIII TCCGGA 1 cut(s) 1732
AclWI GGATC 7 cut(s) 134, 147, 1917, 2067, 2262, 2398, 3122
AcoI YGGCCR 4 cut(s) 265, 777, 2815, 2831
AcsI RAATTY 4 cut(s) 1221, 1855, 2088, 2698
AcuI CTGAAG 5 cut(s) 106, 569, 785, 962, 1683
AcyI GRCGYC 4 cut(s) 108, 204, 881, 3410
AfaI GTAC 4 cut(s) 518, 605, 1029, 2901
AfeI AGCGCT 1 cut(s) 2458
AfiI CCNNNNNNNGG 9 cut(s) 123, 195, 336, 428, 670, 1091, 1307, 3036, 3217
AflII CTTAAG 1 cut(s) 1118
AflIII ACRYGT 2 cut(s) 947, 2652
AhdI GACNNNNNGTC 2 cut(s) 3256, 3409
AhlI ACTAGT 1 cut(s) 2438
AjiI CACGTC 1 cut(s) 2911
AjnI CCWGG 5 cut(s) 135, 684, 2092, 2113, 3232
AloI GAACNNNNNNTCC 2 cut(s) 1265, 1297
Alw21I GWGCWC 4 cut(s) 240, 501, 790, 961
Alw26I GTCTC 7 cut(s) 235, 932, 1322, 1647, 2140, 2507, 3058
AlwI GGATC 7 cut(s) 134, 147, 1917, 2067, 2262, 2398, 3122
AlwNI CAGNNNCTG 1 cut(s) 655
Ama87I CYCGRG 5 cut(s) 225, 1102, 1285, 3242, 3254
Aor13HI TCCGGA 1 cut(s) 1732
Aor51HI AGCGCT 1 cut(s) 2458
ApoI RAATTY 4 cut(s) 1221, 1855, 2088, 2698
AseI ATTAAT 1 cut(s) 2285
Asp700I GAANNNNTTC 3 cut(s) 329, 539, 827
Asp718I GGTACC 1 cut(s) 1027
AsuC2I CCSGG 4 cut(s) 409, 442, 1086, 3374
AsuII TTCGAA 1 cut(s) 2946
AvaI CYCGRG 5 cut(s) 225, 1102, 1285, 3242, 3254
AvaII GGWCC 8 cut(s) 673, 841, 1145, 1411, 1796, 2729, 3328, 3380
AxyI CCTNAGG 2 cut(s) 1371, 2741
BaeI ACNNNNGTAYC 2 cut(s) 1011, 1044
BalI TGGCCA 1 cut(s) 2833
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 6 cut(s) 1027, 1078, 1513, 1585, 1868, 3313
BanII GRGCYC 3 cut(s) 240, 501, 790
BauI CACGAG 2 cut(s) 512, 2865
BbsI GAAGAC 4 cut(s) 376, 2411, 3171, 3177
Bbv12I GWGCWC 4 cut(s) 240, 501, 790, 961
BccI CCATC 9 cut(s) 664, 788, 853, 1117, 1454, 1675, 2361, 2594, 2624
BceAI ACGGC 8 cut(s) 175, 365, 685, 894, 2433, 2830, 2920, 3327
BcgI CGANNNNNNTGC 4 cut(s) 734, 768, 2501, 2535
BciT130I CCWGG 5 cut(s) 137, 686, 2094, 2115, 3234
BcnI CCSGG 4 cut(s) 409, 442, 1086, 3374
BcoDI GTCTC 7 cut(s) 235, 932, 1322, 1647, 2140, 2507, 3058
BcuI ACTAGT 1 cut(s) 2438
BfmI CTRYAG 2 cut(s) 446, 656
BfoI RGCGCY 2 cut(s) 728, 2460
BfrI CTTAAG 1 cut(s) 1118
BglI GCCNNNNNGGC 1 cut(s) 480
BlpI GCTNAGC 2 cut(s) 311, 2555
Bme1390I CCNGG 9 cut(s) 137, 409, 442, 686, 1086, 2094, 2115, 3234, 3374
Bme18I GGWCC 8 cut(s) 673, 841, 1145, 1411, 1796, 2729, 3328, 3380
BmeRI GACNNNNNGTC 2 cut(s) 3256, 3409
BmeT110I CYCGRG 5 cut(s) 225, 1102, 1285, 3242, 3254
BmgBI CACGTC 1 cut(s) 2911
BmrFI CCNGG 9 cut(s) 137, 409, 442, 686, 1086, 2094, 2115, 3234, 3374
BmsI GCATC 6 cut(s) 573, 2114, 2204, 2623, 2810, 2926
BpiI GAAGAC 4 cut(s) 376, 2411, 3171, 3177
BpmI CTGGAG 6 cut(s) 119, 632, 1346, 1671, 1829, 2418
Bpu10I CCTNAGC 2 cut(s) 887, 2431
Bpu1102I GCTNAGC 2 cut(s) 311, 2555
Bpu14I TTCGAA 1 cut(s) 2946
BpuEI CTTGAG 2 cut(s) 1882, 2383
BpuMI CCSGG 4 cut(s) 409, 442, 1086, 3374
Bsa29I ATCGAT 1 cut(s) 1207
BsaBI GATNNNNATC 1 cut(s) 2615
BsaHI GRCGYC 4 cut(s) 108, 204, 881, 3410
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 5 cut(s) 557, 1007, 1085, 1198, 2449
BsaWI WCCGGW 5 cut(s) 318, 617, 1358, 1732, 2694
Bsc4I CCNNNNNNNGG 9 cut(s) 123, 195, 336, 428, 670, 1091, 1307, 3036, 3217
Bse118I RCCGGY 6 cut(s) 464, 545, 2726, 3148, 3330, 3424
Bse1I ACTGG 6 cut(s) 200, 610, 1812, 2061, 2165, 2901
Bse21I CCTNAGG 2 cut(s) 1371, 2741
Bse3DI GCAATG 6 cut(s) 868, 1175, 1501, 1623, 1628, 2847
Bse8I GATNNNNATC 1 cut(s) 2615
BseAI TCCGGA 1 cut(s) 1732
BseBI CCWGG 5 cut(s) 137, 686, 2094, 2115, 3234
BseCI ATCGAT 1 cut(s) 1207
BseDI CCNNGG 5 cut(s) 557, 1007, 1085, 1198, 2449
BseGI GGATG 6 cut(s) 675, 845, 1054, 2605, 2616, 3260
BseJI GATNNNNATC 1 cut(s) 2615
BseLI CCNNNNNNNGG 9 cut(s) 123, 195, 336, 428, 670, 1091, 1307, 3036, 3217
BseMI GCAATG 6 cut(s) 868, 1175, 1501, 1623, 1628, 2847
BseNI ACTGG 6 cut(s) 200, 610, 1812, 2061, 2165, 2901
BseRI GAGGAG 3 cut(s) 520, 574, 1388
BseX3I CGGCCG 1 cut(s) 265
BseYI CCCAGC 2 cut(s) 75, 162
BsgI GTGCAG 1 cut(s) 3292
Bsh1236I CGCG 6 cut(s) 201, 287, 370, 378, 569, 722
Bsh1285I CGRYCG 2 cut(s) 268, 323
BshNI GGYRCC 6 cut(s) 1027, 1078, 1513, 1585, 1868, 3313
BshVI ATCGAT 1 cut(s) 1207
BsiEI CGRYCG 2 cut(s) 268, 323
BsiHKAI GWGCWC 4 cut(s) 240, 501, 790, 961
BsiHKCI CYCGRG 5 cut(s) 225, 1102, 1285, 3242, 3254
BslFI GGGAC 2 cut(s) 827, 3052
BslI CCNNNNNNNGG 9 cut(s) 123, 195, 336, 428, 670, 1091, 1307, 3036, 3217
BsmAI GTCTC 7 cut(s) 235, 932, 1322, 1647, 2140, 2507, 3058
BsmBI CGTCTC 2 cut(s) 1647, 3058
BsmFI GGGAC 2 cut(s) 827, 3052
BsmI GAATGC 1 cut(s) 757
Bso31I GGTCTC 1 cut(s) 235
BsoBI CYCGRG 5 cut(s) 225, 1102, 1285, 3242, 3254
Bsp119I TTCGAA 1 cut(s) 2946
Bsp1286I GDGCHC 4 cut(s) 240, 501, 790, 961
Bsp13I TCCGGA 1 cut(s) 1732
Bsp1720I GCTNAGC 2 cut(s) 311, 2555
Bsp68I TCGCGA 2 cut(s) 287, 569
BspDI ATCGAT 1 cut(s) 1207
BspEI TCCGGA 1 cut(s) 1732
BspFNI CGCG 6 cut(s) 201, 287, 370, 378, 569, 722
BspMAI CTGCAG 2 cut(s) 450, 660
BspPI GGATC 7 cut(s) 134, 147, 1917, 2067, 2262, 2398, 3122
BspT104I TTCGAA 1 cut(s) 2946
BspT107I GGYRCC 6 cut(s) 1027, 1078, 1513, 1585, 1868, 3313
BspTI CTTAAG 1 cut(s) 1118
BspTNI GGTCTC 1 cut(s) 235
BsrBI CCGCTC 2 cut(s) 185, 2082
BsrDI GCAATG 6 cut(s) 868, 1175, 1501, 1623, 1628, 2847
BsrFI RCCGGY 6 cut(s) 464, 545, 2726, 3148, 3330, 3424
BsrI ACTGG 6 cut(s) 200, 610, 1812, 2061, 2165, 2901
BssAI RCCGGY 6 cut(s) 464, 545, 2726, 3148, 3330, 3424
BssECI CCNNGG 5 cut(s) 557, 1007, 1085, 1198, 2449
BssNAI GTATAC 1 cut(s) 2018
BssNI GRCGYC 4 cut(s) 108, 204, 881, 3410
BssSI CACGAG 2 cut(s) 512, 2865
BssT1I CCWWGG 2 cut(s) 1007, 1198
Bst1107I GTATAC 1 cut(s) 2018
Bst2BI CACGAG 2 cut(s) 512, 2865
Bst2UI CCWGG 5 cut(s) 137, 686, 2094, 2115, 3234
Bst4CI ACNGT 6 cut(s) 1042, 2156, 2707, 2774, 3034, 3420
Bst6I CTCTTC 1 cut(s) 3267
BstACI GRCGYC 4 cut(s) 108, 204, 881, 3410
BstAFI CTTAAG 1 cut(s) 1118
BstBI TTCGAA 1 cut(s) 2946
BstDSI CCRYGG 1 cut(s) 2449
BstEII GGTNACC 3 cut(s) 467, 1605, 2774
BstF5I GGATG 6 cut(s) 675, 845, 1054, 2605, 2616, 3260
BstFNI CGCG 6 cut(s) 201, 287, 370, 378, 569, 722
BstH2I RGCGCY 2 cut(s) 728, 2460
BstMAI GTCTC 7 cut(s) 235, 932, 1322, 1647, 2140, 2507, 3058
BstMCI CGRYCG 2 cut(s) 268, 323
BstNI CCWGG 5 cut(s) 137, 686, 2094, 2115, 3234
BstNSI RCATGY 3 cut(s) 951, 2656, 2767
BstPI GGTNACC 3 cut(s) 467, 1605, 2774
BstSCI CCNGG 9 cut(s) 135, 407, 440, 684, 1084, 2092, 2113, 3232, 3372
BstSFI CTRYAG 2 cut(s) 446, 656
BstUI CGCG 6 cut(s) 201, 287, 370, 378, 569, 722
BstV2I GAAGAC 4 cut(s) 376, 2411, 3171, 3177
BstX2I RGATCY 4 cut(s) 139, 1909, 2059, 2254
BstYI RGATCY 4 cut(s) 139, 1909, 2059, 2254
BstZ17I GTATAC 1 cut(s) 2018
BstZI CGGCCG 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 1207
Bsu36I CCTNAGG 2 cut(s) 1371, 2741
BsuTUI ATCGAT 1 cut(s) 1207
BtgI CCRYGG 1 cut(s) 2449
BtgZI GCGATG 1 cut(s) 550
BtrI CACGTC 1 cut(s) 2911
BtsCI GGATG 6 cut(s) 675, 845, 1054, 2605, 2616, 3260
BtsI GCAGTG 1 cut(s) 2422
BtsIMutI CAGTG 9 cut(s) 280, 1047, 1423, 1805, 1905, 2054, 2172, 2422, 2894
BtuMI TCGCGA 2 cut(s) 287, 569
CaiI CAGNNNCTG 1 cut(s) 655
Cfr10I RCCGGY 6 cut(s) 464, 545, 2726, 3148, 3330, 3424
ClaI ATCGAT 1 cut(s) 1207
CpoI CGGWCCG 1 cut(s) 1145
CseI GACGC 6 cut(s) 116, 193, 730, 870, 2179, 2828
Csp6I GTAC 4 cut(s) 517, 604, 1028, 2900
CspI CGGWCCG 1 cut(s) 1145
CviQI GTAC 4 cut(s) 517, 604, 1028, 2900
DrdI GACNNNNNNGTC 1 cut(s) 2819
DriI GACNNNNNGTC 2 cut(s) 3256, 3409
DseDI GACNNNNNNGTC 1 cut(s) 2819
EaeI YGGCCR 4 cut(s) 265, 777, 2815, 2831
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 3267
Eam1105I GACNNNNNGTC 2 cut(s) 3256, 3409
EarI CTCTTC 1 cut(s) 3267
EciI GGCGGA 1 cut(s) 3388
Ecl136II GAGCTC 3 cut(s) 238, 499, 788
EclXI CGGCCG 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 1007, 1198
Eco24I GRGCYC 3 cut(s) 240, 501, 790
Eco31I GGTCTC 1 cut(s) 235
Eco47I GGWCC 8 cut(s) 673, 841, 1145, 1411, 1796, 2729, 3328, 3380
Eco47III AGCGCT 1 cut(s) 2458
Eco52I CGGCCG 1 cut(s) 265
Eco53kI GAGCTC 3 cut(s) 238, 499, 788
Eco57I CTGAAG 5 cut(s) 106, 569, 785, 962, 1683
Eco81I CCTNAGG 2 cut(s) 1371, 2741
Eco88I CYCGRG 5 cut(s) 225, 1102, 1285, 3242, 3254
Eco91I GGTNACC 3 cut(s) 467, 1605, 2774
EcoICRI GAGCTC 3 cut(s) 238, 499, 788
EcoO65I GGTNACC 3 cut(s) 467, 1605, 2774
EcoRI GAATTC 1 cut(s) 1855
EcoRII CCWGG 5 cut(s) 135, 684, 2092, 2113, 3232
EcoT14I CCWWGG 2 cut(s) 1007, 1198
EcoT22I ATGCAT 2 cut(s) 1717, 2197
EcoT38I GRGCYC 3 cut(s) 240, 501, 790
ErhI CCWWGG 2 cut(s) 1007, 1198
Esp3I CGTCTC 2 cut(s) 1647, 3058
FaqI GGGAC 2 cut(s) 827, 3052
FauI CCCGC 2 cut(s) 2075, 3330
FauNDI CATATG 1 cut(s) 2569
FblI GTMKAC 1 cut(s) 2017
FokI GGATG 6 cut(s) 682, 832, 1061, 2603, 2612, 3247
FriOI GRGCYC 3 cut(s) 240, 501, 790
GsaI CCCAGC 2 cut(s) 79, 166
GsuI CTGGAG 6 cut(s) 119, 632, 1346, 1671, 1829, 2418
HaeII RGCGCY 2 cut(s) 728, 2460
HgaI GACGC 6 cut(s) 116, 193, 730, 870, 2179, 2828
Hin1I GRCGYC 4 cut(s) 108, 204, 881, 3410
HindIII AAGCTT 2 cut(s) 797, 2477
Hpy166II GTNNAC 8 cut(s) 517, 918, 2018, 2125, 2732, 2902, 3103, 3159
Hpy8I GTNNAC 8 cut(s) 517, 918, 2018, 2125, 2732, 2902, 3103, 3159
HpyCH4III ACNGT 6 cut(s) 1042, 2156, 2707, 2774, 3034, 3420
HpyCH4IV ACGT 4 cut(s) 1656, 2910, 3051, 3410
HpySE526I ACGT 4 cut(s) 1656, 2910, 3051, 3410
Hsp92I GRCGYC 4 cut(s) 108, 204, 881, 3410
Kpn2I TCCGGA 1 cut(s) 1732
KpnI GGTACC 1 cut(s) 1031
KroI GCCGGC 2 cut(s) 545, 3424
KroNI GCCGGC 2 cut(s) 547, 3426
LweI GCATC 6 cut(s) 573, 2114, 2204, 2623, 2810, 2926
MaeII ACGT 4 cut(s) 1656, 2910, 3051, 3410
MbiI CCGCTC 2 cut(s) 185, 2082
MfeI CAATTG 3 cut(s) 715, 2517, 2827
MflI RGATCY 4 cut(s) 139, 1909, 2059, 2254
MhlI GDGCHC 4 cut(s) 240, 501, 790, 961
MlsI TGGCCA 1 cut(s) 2833
MluNI TGGCCA 1 cut(s) 2833
MmeI TCCRAC 7 cut(s) 280, 1026, 1803, 1971, 3032, 3358, 3369
Mox20I TGGCCA 1 cut(s) 2833
Mph1103I ATGCAT 2 cut(s) 1717, 2197
MreI CGCCGGCG 1 cut(s) 545
MroI TCCGGA 1 cut(s) 1732
MroNI GCCGGC 2 cut(s) 545, 3424
MroXI GAANNNNTTC 3 cut(s) 329, 539, 827
MscI TGGCCA 1 cut(s) 2833
MslI CAYNNNNRTG 2 cut(s) 2609, 3265
Msp20I TGGCCA 1 cut(s) 2833
MspA1I CMGCKG 2 cut(s) 655, 3325
MspCI CTTAAG 1 cut(s) 1118
MspR9I CCNGG 9 cut(s) 137, 409, 442, 686, 1086, 2094, 2115, 3234, 3374
MteI GCGCNGCGC 1 cut(s) 723
MunI CAATTG 3 cut(s) 715, 2517, 2827
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 5 cut(s) 137, 686, 2094, 2115, 3234
MvnI CGCG 6 cut(s) 201, 287, 370, 378, 569, 722
NaeI GCCGGC 2 cut(s) 547, 3426
NciI CCSGG 4 cut(s) 409, 442, 1086, 3374
NdeI CATATG 1 cut(s) 2569
NgoMIV GCCGGC 2 cut(s) 545, 3424
NmeAIII GCCGAG 3 cut(s) 124, 240, 738
NmuCI GTSAC 5 cut(s) 275, 1724, 2167, 3082, 3214
NruI TCGCGA 2 cut(s) 287, 569
NsiI ATGCAT 2 cut(s) 1717, 2197
NspI RCATGY 3 cut(s) 951, 2656, 2767
NspV TTCGAA 1 cut(s) 2946
PaeR7I CTCGAG 2 cut(s) 1102, 3242
PciI ACATGT 2 cut(s) 947, 2652
PcsI WCGNNNNNNNCGW 4 cut(s) 519, 3057, 3123, 3416
PctI GAATGC 1 cut(s) 757
PdiI GCCGGC 2 cut(s) 547, 3426
PdmI GAANNNNTTC 3 cut(s) 329, 539, 827
PfoI TCCNGGA 3 cut(s) 135, 407, 684
PscI ACATGT 2 cut(s) 947, 2652
PshBI ATTAAT 1 cut(s) 2285
Psp124BI GAGCTC 3 cut(s) 240, 501, 790
Psp6I CCWGG 5 cut(s) 135, 684, 2092, 2113, 3232
PspEI GGTNACC 3 cut(s) 467, 1605, 2774
PspFI CCCAGC 2 cut(s) 75, 162
PspGI CCWGG 5 cut(s) 135, 684, 2092, 2113, 3232
PspXI VCTCGAGB 1 cut(s) 3242
PstI CTGCAG 2 cut(s) 450, 660
PstNI CAGNNNCTG 1 cut(s) 655
PsuI RGATCY 4 cut(s) 139, 1909, 2059, 2254
PvuII CAGCTG 1 cut(s) 655
RruI TCGCGA 2 cut(s) 287, 569
RsaI GTAC 4 cut(s) 518, 605, 1029, 2901
RsaNI GTAC 4 cut(s) 517, 604, 1028, 2900
RseI CAYNNNNRTG 2 cut(s) 2609, 3265
Rsr2I CGGWCCG 1 cut(s) 1145
RsrII CGGWCCG 1 cut(s) 1145
SacI GAGCTC 3 cut(s) 240, 501, 790
ScrFI CCNGG 9 cut(s) 137, 409, 442, 686, 1086, 2094, 2115, 3234, 3374
SduI GDGCHC 4 cut(s) 240, 501, 790, 961
SfaNI GCATC 6 cut(s) 573, 2114, 2204, 2623, 2810, 2926
SfcI CTRYAG 2 cut(s) 446, 656
Sfr274I CTCGAG 2 cut(s) 1102, 3242
SfuI TTCGAA 1 cut(s) 2946
SgrAI CRCCGGYG 1 cut(s) 545
SinI GGWCC 8 cut(s) 673, 841, 1145, 1411, 1796, 2729, 3328, 3380
SlaI CTCGAG 2 cut(s) 1102, 3242
SmiMI CAYNNNNRTG 2 cut(s) 2609, 3265
SmlI CTYRAG 5 cut(s) 1102, 1118, 1861, 2362, 3242
SmoI CTYRAG 5 cut(s) 1102, 1118, 1861, 2362, 3242
SpeI ACTAGT 1 cut(s) 2438
SstI GAGCTC 3 cut(s) 240, 501, 790
StyD4I CCNGG 9 cut(s) 135, 407, 440, 684, 1084, 2092, 2113, 3232, 3372
StyI CCWWGG 2 cut(s) 1007, 1198
TaaI ACNGT 6 cut(s) 1042, 2156, 2707, 2774, 3034, 3420
TaiI ACGT 4 cut(s) 1659, 2913, 3054, 3413
TaqII GACCGA 1 cut(s) 1743
TscAI CASTG 9 cut(s) 280, 1047, 1423, 1812, 1905, 2061, 2172, 2422, 2901
TseFI GTSAC 5 cut(s) 275, 1724, 2167, 3082, 3214
Tsp45I GTSAC 5 cut(s) 275, 1724, 2167, 3082, 3214
TspDTI ATGAA 7 cut(s) 17, 1125, 1761, 1868, 2156, 2661, 3346
TspGWI ACGGA 3 cut(s) 935, 2609, 3233
TspRI CASTG 9 cut(s) 280, 1047, 1423, 1812, 1905, 2061, 2172, 2422, 2901
Vha464I CTTAAG 1 cut(s) 1118
VpaK11BI GGWCC 8 cut(s) 673, 841, 1145, 1411, 1796, 2729, 3328, 3380
VspI ATTAAT 1 cut(s) 2285
XapI RAATTY 4 cut(s) 1221, 1855, 2088, 2698
XbaI TCTAGA 1 cut(s) 1840
XceI RCATGY 3 cut(s) 951, 2656, 2767
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XhoI CTCGAG 2 cut(s) 1102, 3242
XmiI GTMKAC 1 cut(s) 2017
XmnI GAANNNNTTC 3 cut(s) 329, 539, 827
ZraI GACGTC 1 cut(s) 3411
Zsp2I ATGCAT 2 cut(s) 1717, 2197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.