Rroxscaffold_3G00232650
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015871
Physical Location & Seq
Reverse (-)
18307808 .. 18311242
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232650.1

Sequence Viewer

Length: 3027 bp
ATGAAACCCATTATGCCTGCGCTTCTTCTTTTCTTTCTTCTGGTGCTTTCCGGCGCACCCTTCTTCTCCTGCGCCGCCCAGCGCAGCCCCGAAAGCCTAGCCGAGATCGACGCCCTCACCACCTTCAGGCTCGACCTCCAGGATCCGATTGGCGCATTGAACGGCTGGGATTCCTCCACGCCTTCCGCTCCCTGCGACTGGCGCGGCGTCTTTTGCGACAGTAGTAGTCGGGTCTCCGAGCTCCGCTTGCCTCGCCTCCAACTCGGCGGCCGACTCAGTGACTCGCTCGCGAAGCTCACCATGCTGGGGAAGCTCAGCCTCCGCTCAAACTCCTTCAACGGGAGCATTCCTGATTCGCTTTCCCAATGCACGCGCCTACGCGCCGTCTTCCTGCAGAACAACTCTCTCTCCGGGAAGCTCCCGCCGGGGATCGGGAACCTGACCGCGCTGCAGATTCTCAATCTCCAGCAGCTGCAGTTCCTCTGGCTGGACCACAACCTCCTGGAAGGAACGCTGCCGTCGGCGATAGCCAATTGCGCCGCGCTGGTGCATTTCAGCGTCGAAGGGAATGCGCTCGGCGGCGTGATTCCGGCGGCTATCGGAGCTCTGCCGAAGCTTCAGGTGTTGTCGCTTTCACAGAATAATTTCTCTGGTATGGTCCCATCCTCGTTGTTTTGCAATGCCTCATTCAATACGGCGTCGCTTAGGATTGTCCAGCTAGGGTTTAATTCGTTCACGGATATCGTTAAGCCTGAGACGGCCACATGTTTTAGTGCTCTGGTGGTTTTGGATCTTCAGCACAATCAGATTGGCGGCGATTTTCCCTGGTGGATAATAACGCAGGTACCAAACTTGACAGTGTTGGATGTTTCTAGTAACTCATTCTCCGGCGTGGTGCCGCCGGGGATTGGAAACCTCTCGAGGTTACAGGAGCTTAAGATGGCTAATAATTCATTTAGCGGTTTGAAGATGCTATCTTTAGGGGAAAATCAGTTCTGGGGTTCCATTCCTCCGAGTTTTGGTAATCTTACGAGGCTGGAGACGCTGGGATTGGGAGGGAACAACTTGACCGGAACTATGCCTGAGGAGTTGTTAATAGGGTTGGGGGTGCCGACTAGCTTGGGTAGTCTTTTCAGGCTTACTACTATTGATCTGAGCAAACAAAACTTCTCTGGTGAGGTGCCTTCTGAGCTTTTGGGGTTACCCAATTTACAAGTCATTGCATTGCAAGAGAACAGGTTGTCTGGAGACGTTCCTGAAGGTTTTAGTAGCTTGATGGGTCTGCATTATTTGAATCTCAGTTCAAATGCATTTTCTGGTCACATTCCGGAGAATTATGGTTTTCTTCGGTCATTGGTTGTTCTTTCATTGTCCAACAATCACATTTCAGGACCAATTCCACCGGAGCTTGGAAACTGCTCTGATCTCCAAGTTCTAGAGCTGCAAATGAATTCCTTGACTGGCACCATTCCAGCCGATCTCTCTCGCCTATCAGTGTTAAAGGATCTTGATTTGGGTAGCAACAACTTAACAGGTAGAATCCCAGAGGAAATGTCTAAATGCTCATCATTAACTGCTTTGTTGTTGGACTCCAATCATCTCTCAGGTGGTATACCAGACTCGTTGTCTAAGCTATTGAACCTAACCACACTGGATCTCTCCAATAACAATTTGAGCGGGCAAATTCCTGGTGATCTTGCATCTATTCCTGGCCTAGTGAACTTCAATGTCTCGATGAATAACCTCGACGGTGAGATTCCAGTGACGCTTGGTTCTCGGTTCAACAATGCATCAGACTTTTCAGGGAATCAAAACTTATGTGGGCCGCCATTGGAAAAGAAGTGCAAGGATCTAGACAAGAAAGATAGAAATAAGAGATTAATTCTTTTGATTGTGATTGTTGTTAGTGGAGCTTGCCTATTAGCTTTGTGCTGCTGCTTCTACATTTTCAGCCTCTTGAGATGGCGCAAAAAGCTCAAACAGGGATCATCTGGAGAGAAGACTAGAAGCAGTGCGAGGGCAAGCTCAGGAACTAGTGAGGGCCGTGGAAGCTCTGATAATGGAGTGCCAAAGCTTGTCATGTTCAACAGCAAAATCACACTTGCAGAGACAATTGCAGCCACAAGGCAATTCGATGAAGAAAATGTGCTGAGCAGATCACCATATGGCTTGGTTTTCAAGGCTTGTTATGAGGATGGTATGGTGATGTCCATCCGTCGCCTCCCCGATGTGACACTCGATGAAAACATGTTCCGGAAAGAAGCAGAATCACTAGGCAGAGTCAAGCACCGGAATTTAACAGTTCTTCGAGGCTACTATGCCGGTCCACCTGACCTCAGGCTTCTAATCTATGACTACATGCCCAACGGTAACCTAGCCACCCTTCTGCAAGAAGCATCACACCAAGATGGCCATGTCCTCAATTGGCCAATGCGCCATCTCATTGCACTTGGAATTGCTCGTGGCTTGGCTTTTCTACACACATCTTCACTGGTACACGGCGACGTGAAGCCACAAAGCGTGCTATTCGATGCAGATTTCGAAGCCCATTTGTCTGATTTCGGCCTAGACAAACTCACATCAGCAACCAGCACTACTACCCCAACCGAAGCCTCAACCTCAACCACAGTCGGGACATTAGGCTACGTCTCTCACGAAGCAACACTAACAGGCAGAGTCACAAAGGAATCCGATGTTTACAGCTACGGGATCGTCTTGCTTGAGCTCTTAACAGGAAAGAGGCCGGTGATGTTCACCGAAGACGAAGACATAGTGAAGTGGGTGAAGAAGCAGCTTCAAAGAGGCCAAGTCACGGAGCTACTAGAGCCAGGGCTGCTCGAGCTTGACCCCGAGTCATCCGAGTGGGAAGAGTTCTTGCTTGGCGTGAAAGTCGGGTTGCTTTGCACGGCACCCGACCCGCTGGACCGGCCCACCATGTCCGACATTGTTTTCATGCTCGAAGGCTGCCGGGTCGGACCCGATCTCCCGTCCTCCGCCGACCCGACAACGCAACCGTCGCCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1008

Amino Acids

108.88

Weight (kDa)

5.34

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 73 1.2e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 373 - 601 2.2e-12 Leucine-rich repeat region
LRR_8 PF13855 379 - 437 3e-07 Leucine rich repeat
LRR_8 PF13855 476 - 534 5.3e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 716 - 933 3.8e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 721 - 982 1.4e-29 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36180
fragaria_vesca FvH4_5g31360
prunus_persica Prupe.7G166100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0226621
rosa_laevigata RLG00000001713
rosa_multiflora Rmu_co8455755.1_g000001 Rmu_sc0002043.1_g000006 Rmu_sc0004294.1_g000002
rosa_roxburghii Rroxscaffold_3G00232650
rosa_rugosa Rorug07G0238700
rosa_samantha Rh7AG386200 Rh7BG372900 Rh7CG405500 Rh7DG384900
rosa_wichuraiana Rw7G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 832
Acc65I GGTACC 1 cut(s) 844
AccB1I GGYRCC 6 cut(s) 844, 895, 1108, 1180, 1463, 2908
AccBSI CCGCTC 3 cut(s) 188, 324, 1677
AccI GTMKAC 1 cut(s) 1612
AccII CGCG 6 cut(s) 204, 290, 373, 381, 446, 542
AccIII TCCGGA 2 cut(s) 1327, 2253
AclWI GGATC 9 cut(s) 137, 150, 437, 798, 1512, 1662, 1857, 1993, 2717
AcoI YGGCCR 4 cut(s) 268, 759, 2410, 2426
AcsI RAATTY 3 cut(s) 1450, 1683, 2293
AcuI CTGAAG 4 cut(s) 109, 602, 779, 1278
AcyI GRCGYC 3 cut(s) 111, 207, 698
AfaI GTAC 2 cut(s) 846, 2496
AflII CTTAAG 1 cut(s) 935
AflIII ACRYGT 2 cut(s) 764, 2247
AhdI GACNNNNNGTC 2 cut(s) 1624, 2851
AhlI ACTAGT 1 cut(s) 2033
AjiI CACGTC 1 cut(s) 2506
AjnI CCWGG 6 cut(s) 138, 501, 824, 1687, 1708, 2827
AloI GAACNNNNNNTCC 2 cut(s) 977, 1009
Alw21I GWGCWC 4 cut(s) 243, 607, 778, 2727
Alw26I GTCTC 7 cut(s) 238, 749, 1034, 1242, 1735, 2102, 2653
AlwI GGATC 9 cut(s) 137, 150, 437, 798, 1512, 1662, 1857, 1993, 2717
AlwNI CAGNNNCTG 1 cut(s) 472
Ama87I CYCGRG 3 cut(s) 919, 2837, 2849
Aor13HI TCCGGA 2 cut(s) 1327, 2253
ApoI RAATTY 3 cut(s) 1450, 1683, 2293
AseI ATTAAT 1 cut(s) 1880
Asp700I GAANNNNTTC 1 cut(s) 644
Asp718I GGTACC 1 cut(s) 844
AspS9I GGNCC 9 cut(s) 490, 658, 1391, 1823, 2041, 2324, 2923, 2928, 2975
AsuC2I CCSGG 4 cut(s) 412, 426, 903, 2969
AsuII TTCGAA 1 cut(s) 2541
AvaI CYCGRG 3 cut(s) 919, 2837, 2849
AvaII GGWCC 6 cut(s) 490, 658, 1391, 2324, 2923, 2975
AxyI CCTNAGG 2 cut(s) 1083, 2336
BaeI ACNNNNGTAYC 2 cut(s) 828, 861
BalI TGGCCA 2 cut(s) 2412, 2428
BamHI GGATCC 1 cut(s) 142
BanI GGYRCC 6 cut(s) 844, 895, 1108, 1180, 1463, 2908
BanII GRGCYC 3 cut(s) 243, 607, 2727
BauI CACGAG 1 cut(s) 2460
BbsI GAAGAC 4 cut(s) 379, 2006, 2766, 2772
Bbv12I GWGCWC 4 cut(s) 243, 607, 778, 2727
BccI CCATC 8 cut(s) 670, 934, 1270, 1956, 2189, 2219, 2402, 2445
BceAI ACGGC 8 cut(s) 178, 368, 502, 711, 774, 2028, 2515, 2922
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 6 cut(s) 140, 503, 826, 1689, 1710, 2829
BcnI CCSGG 4 cut(s) 412, 426, 903, 2969
BcoDI GTCTC 7 cut(s) 238, 749, 1034, 1242, 1735, 2102, 2653
BcuI ACTAGT 1 cut(s) 2033
BfmI CTRYAG 3 cut(s) 392, 449, 473
BfrI CTTAAG 1 cut(s) 935
BfuAI ACCTGC 1 cut(s) 832
BlpI GCTNAGC 2 cut(s) 314, 2150
Bme18I GGWCC 6 cut(s) 490, 658, 1391, 2324, 2923, 2975
BmeRI GACNNNNNGTC 2 cut(s) 1624, 2851
BmeT110I CYCGRG 3 cut(s) 919, 2837, 2849
BmgBI CACGTC 1 cut(s) 2506
BmgT120I GGNCC 9 cut(s) 490, 658, 1391, 1823, 2041, 2324, 2923, 2928, 2975
BmsI GCATC 5 cut(s) 960, 1709, 1799, 2405, 2521
BpiI GAAGAC 4 cut(s) 379, 2006, 2766, 2772
BpmI CTGGAG 5 cut(s) 122, 449, 1058, 1266, 2013
Bpu10I CCTNAGC 2 cut(s) 704, 2026
Bpu1102I GCTNAGC 2 cut(s) 314, 2150
Bpu14I TTCGAA 1 cut(s) 2541
BpuEI CTTGAG 2 cut(s) 1978, 2741
BpuMI CCSGG 4 cut(s) 412, 426, 903, 2969
BsaBI GATNNNNATC 1 cut(s) 2210
BsaHI GRCGYC 3 cut(s) 111, 207, 698
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 5 cut(s) 425, 824, 902, 2044, 2828
BsaWI WCCGGW 5 cut(s) 1070, 1327, 1402, 2253, 2289
BsaXI ACNNNNNCTCC 6 cut(s) 392, 422, 869, 899, 2967, 2997
Bse118I RCCGGY 4 cut(s) 2321, 2743, 2925, 3019
Bse1I ACTGG 5 cut(s) 203, 1465, 1656, 1760, 2496
Bse21I CCTNAGG 2 cut(s) 1083, 2336
Bse3DI GCAATG 4 cut(s) 685, 1218, 1223, 2442
Bse8I GATNNNNATC 1 cut(s) 2210
BseAI TCCGGA 2 cut(s) 1327, 2253
BseBI CCWGG 6 cut(s) 140, 503, 826, 1689, 1710, 2829
BseDI CCNNGG 5 cut(s) 425, 824, 902, 2044, 2828
BseGI GGATG 5 cut(s) 662, 871, 2200, 2211, 2855
BseJI GATNNNNATC 1 cut(s) 2210
BseMI GCAATG 4 cut(s) 685, 1218, 1223, 2442
BseNI ACTGG 5 cut(s) 203, 1465, 1656, 1760, 2496
BseRI GAGGAG 1 cut(s) 1100
BseX3I CGGCCG 1 cut(s) 268
BseYI CCCAGC 4 cut(s) 78, 165, 304, 1045
Bsh1236I CGCG 6 cut(s) 204, 290, 373, 381, 446, 542
Bsh1285I CGRYCG 1 cut(s) 271
BshNI GGYRCC 6 cut(s) 844, 895, 1108, 1180, 1463, 2908
BsiEI CGRYCG 1 cut(s) 271
BsiHKAI GWGCWC 4 cut(s) 243, 607, 778, 2727
BsiHKCI CYCGRG 3 cut(s) 919, 2837, 2849
BslFI GGGAC 2 cut(s) 644, 2647
BsmAI GTCTC 7 cut(s) 238, 749, 1034, 1242, 1735, 2102, 2653
BsmBI CGTCTC 4 cut(s) 749, 1034, 1242, 2653
BsmFI GGGAC 2 cut(s) 644, 2647
BsmI GAATGC 2 cut(s) 345, 574
Bso31I GGTCTC 1 cut(s) 238
BsoBI CYCGRG 3 cut(s) 919, 2837, 2849
Bsp119I TTCGAA 1 cut(s) 2541
Bsp1286I GDGCHC 4 cut(s) 243, 607, 778, 2727
Bsp13I TCCGGA 2 cut(s) 1327, 2253
Bsp1720I GCTNAGC 2 cut(s) 314, 2150
Bsp68I TCGCGA 1 cut(s) 290
BspEI TCCGGA 2 cut(s) 1327, 2253
BspFNI CGCG 6 cut(s) 204, 290, 373, 381, 446, 542
BspMAI CTGCAG 3 cut(s) 396, 453, 477
BspMI ACCTGC 1 cut(s) 832
BspPI GGATC 9 cut(s) 137, 150, 437, 798, 1512, 1662, 1857, 1993, 2717
BspT104I TTCGAA 1 cut(s) 2541
BspT107I GGYRCC 6 cut(s) 844, 895, 1108, 1180, 1463, 2908
BspTI CTTAAG 1 cut(s) 935
BspTNI GGTCTC 1 cut(s) 238
BsrBI CCGCTC 3 cut(s) 188, 324, 1677
BsrDI GCAATG 4 cut(s) 685, 1218, 1223, 2442
BsrFI RCCGGY 4 cut(s) 2321, 2743, 2925, 3019
BsrI ACTGG 5 cut(s) 203, 1465, 1656, 1760, 2496
BssAI RCCGGY 4 cut(s) 2321, 2743, 2925, 3019
BssECI CCNNGG 5 cut(s) 425, 824, 902, 2044, 2828
BssNAI GTATAC 1 cut(s) 1613
BssNI GRCGYC 3 cut(s) 111, 207, 698
BssSI CACGAG 1 cut(s) 2460
Bst1107I GTATAC 1 cut(s) 1613
Bst2BI CACGAG 1 cut(s) 2460
Bst2UI CCWGG 6 cut(s) 140, 503, 826, 1689, 1710, 2829
Bst4CI ACNGT 7 cut(s) 221, 859, 1751, 2302, 2369, 2629, 3015
Bst6I CTCTTC 1 cut(s) 2862
BstACI GRCGYC 3 cut(s) 111, 207, 698
BstAFI CTTAAG 1 cut(s) 935
BstBI TTCGAA 1 cut(s) 2541
BstC8I GCNNGC 9 cut(s) 18, 248, 288, 371, 1679, 1915, 2023, 2522, 3021
BstDSI CCRYGG 1 cut(s) 2044
BstEII GGTNACC 2 cut(s) 1200, 2369
BstF5I GGATG 5 cut(s) 662, 871, 2200, 2211, 2855
BstFNI CGCG 6 cut(s) 204, 290, 373, 381, 446, 542
BstMAI GTCTC 7 cut(s) 238, 749, 1034, 1242, 1735, 2102, 2653
BstMCI CGRYCG 1 cut(s) 271
BstNI CCWGG 6 cut(s) 140, 503, 826, 1689, 1710, 2829
BstNSI RCATGY 3 cut(s) 768, 2251, 2362
BstPI GGTNACC 2 cut(s) 1200, 2369
BstSFI CTRYAG 3 cut(s) 392, 449, 473
BstUI CGCG 6 cut(s) 204, 290, 373, 381, 446, 542
BstV2I GAAGAC 4 cut(s) 379, 2006, 2766, 2772
BstX2I RGATCY 5 cut(s) 142, 790, 1504, 1654, 1849
BstYI RGATCY 5 cut(s) 142, 790, 1504, 1654, 1849
BstZ17I GTATAC 1 cut(s) 1613
BstZI CGGCCG 1 cut(s) 268
Bsu36I CCTNAGG 2 cut(s) 1083, 2336
BtgI CCRYGG 1 cut(s) 2044
BtrI CACGTC 1 cut(s) 2506
BtsCI GGATG 5 cut(s) 662, 871, 2200, 2211, 2855
BtsI GCAGTG 1 cut(s) 2017
BtsIMutI CAGTG 7 cut(s) 283, 864, 1500, 1649, 1767, 2017, 2489
BtuMI TCGCGA 1 cut(s) 290
BveI ACCTGC 1 cut(s) 832
Cac8I GCNNGC 9 cut(s) 18, 248, 288, 371, 1679, 1915, 2023, 2522, 3021
CaiI CAGNNNCTG 1 cut(s) 472
Cfr10I RCCGGY 4 cut(s) 2321, 2743, 2925, 3019
Cfr13I GGNCC 9 cut(s) 490, 658, 1391, 1823, 2041, 2324, 2923, 2928, 2975
CseI GACGC 6 cut(s) 119, 196, 547, 687, 1051, 1774
Csp6I GTAC 2 cut(s) 845, 2495
CviAII CATG 9 cut(s) 301, 765, 2080, 2248, 2359, 2414, 2935, 2953, 3024
CviQI GTAC 2 cut(s) 845, 2495
DriI GACNNNNNGTC 2 cut(s) 1624, 2851
EaeI YGGCCR 4 cut(s) 268, 759, 2410, 2426
EagI CGGCCG 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 2862
Eam1105I GACNNNNNGTC 2 cut(s) 1624, 2851
EarI CTCTTC 1 cut(s) 2862
EciI GGCGGA 1 cut(s) 2983
Ecl136II GAGCTC 3 cut(s) 241, 605, 2725
EclXI CGGCCG 1 cut(s) 268
Eco24I GRGCYC 3 cut(s) 243, 607, 2727
Eco31I GGTCTC 1 cut(s) 238
Eco32I GATATC 1 cut(s) 742
Eco47I GGWCC 6 cut(s) 490, 658, 1391, 2324, 2923, 2975
Eco52I CGGCCG 1 cut(s) 268
Eco53kI GAGCTC 3 cut(s) 241, 605, 2725
Eco57I CTGAAG 4 cut(s) 109, 602, 779, 1278
Eco81I CCTNAGG 2 cut(s) 1083, 2336
Eco88I CYCGRG 3 cut(s) 919, 2837, 2849
Eco91I GGTNACC 2 cut(s) 1200, 2369
EcoICRI GAGCTC 3 cut(s) 241, 605, 2725
EcoO65I GGTNACC 2 cut(s) 1200, 2369
EcoRI GAATTC 1 cut(s) 1450
EcoRII CCWGG 6 cut(s) 138, 501, 824, 1687, 1708, 2827
EcoRV GATATC 1 cut(s) 742
EcoT22I ATGCAT 2 cut(s) 1312, 1792
EcoT38I GRGCYC 3 cut(s) 243, 607, 2727
Esp3I CGTCTC 4 cut(s) 749, 1034, 1242, 2653
FaeI CATG 9 cut(s) 304, 768, 2083, 2251, 2362, 2417, 2938, 2956, 3027
FaqI GGGAC 2 cut(s) 644, 2647
FatI CATG 9 cut(s) 300, 764, 2079, 2247, 2358, 2413, 2934, 2952, 3023
FauI CCCGC 3 cut(s) 429, 1670, 2925
FauNDI CATATG 1 cut(s) 2164
FblI GTMKAC 1 cut(s) 1612
FokI GGATG 5 cut(s) 649, 878, 2198, 2207, 2842
FriOI GRGCYC 3 cut(s) 243, 607, 2727
GsaI CCCAGC 4 cut(s) 82, 169, 308, 1049
GsuI CTGGAG 5 cut(s) 122, 449, 1058, 1266, 2013
HgaI GACGC 6 cut(s) 119, 196, 547, 687, 1051, 1774
Hin1I GRCGYC 3 cut(s) 111, 207, 698
Hin1II CATG 9 cut(s) 304, 768, 2083, 2251, 2362, 2417, 2938, 2956, 3027
HindIII AAGCTT 2 cut(s) 614, 2072
Hpy166II GTNNAC 7 cut(s) 735, 1613, 1720, 2327, 2497, 2698, 2754
Hpy8I GTNNAC 7 cut(s) 735, 1613, 1720, 2327, 2497, 2698, 2754
Hpy99I CGWCG 8 cut(s) 113, 523, 563, 703, 1751, 2220, 2507, 3019
HpyCH4III ACNGT 7 cut(s) 221, 859, 1751, 2302, 2369, 2629, 3015
HpyCH4IV ACGT 3 cut(s) 1251, 2505, 2646
HpySE526I ACGT 3 cut(s) 1251, 2505, 2646
Hsp92I GRCGYC 3 cut(s) 111, 207, 698
Hsp92II CATG 9 cut(s) 304, 768, 2083, 2251, 2362, 2417, 2938, 2956, 3027
Kpn2I TCCGGA 2 cut(s) 1327, 2253
KpnI GGTACC 1 cut(s) 848
KroI GCCGGC 1 cut(s) 3019
KroNI GCCGGC 1 cut(s) 3021
LmnI GCTCC 9 cut(s) 193, 246, 342, 423, 602, 931, 1405, 1910, 2815
LweI GCATC 5 cut(s) 960, 1709, 1799, 2405, 2521
MaeII ACGT 3 cut(s) 1251, 2505, 2646
MbiI CCGCTC 3 cut(s) 188, 324, 1677
MfeI CAATTG 3 cut(s) 532, 2112, 2422
MflI RGATCY 5 cut(s) 142, 790, 1504, 1654, 1849
MhlI GDGCHC 4 cut(s) 243, 607, 778, 2727
MlsI TGGCCA 2 cut(s) 2412, 2428
MluNI TGGCCA 2 cut(s) 2412, 2428
MlyI GAGTC 7 cut(s) 267, 275, 1583, 1613, 2289, 2685, 2861
MmeI TCCRAC 6 cut(s) 283, 843, 1398, 1566, 2953, 2964
Mox20I TGGCCA 2 cut(s) 2412, 2428
Mph1103I ATGCAT 2 cut(s) 1312, 1792
MroI TCCGGA 2 cut(s) 1327, 2253
MroNI GCCGGC 1 cut(s) 3019
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 2 cut(s) 2412, 2428
MslI CAYNNNNRTG 2 cut(s) 2406, 2860
Msp20I TGGCCA 2 cut(s) 2412, 2428
MspA1I CMGCKG 2 cut(s) 472, 2920
MspCI CTTAAG 1 cut(s) 935
MteI GCGCNGCGC 1 cut(s) 540
MunI CAATTG 3 cut(s) 532, 2112, 2422
Mva1269I GAATGC 2 cut(s) 345, 574
MvaI CCWGG 6 cut(s) 140, 503, 826, 1689, 1710, 2829
MvnI CGCG 6 cut(s) 204, 290, 373, 381, 446, 542
NaeI GCCGGC 1 cut(s) 3021
NciI CCSGG 4 cut(s) 412, 426, 903, 2969
NdeI CATATG 1 cut(s) 2164
NgoMIV GCCGGC 1 cut(s) 3019
NlaIII CATG 9 cut(s) 304, 768, 2083, 2251, 2362, 2417, 2938, 2956, 3027
NmeAIII GCCGAG 3 cut(s) 127, 243, 555
NmuCI GTSAC 6 cut(s) 278, 1319, 1762, 2230, 2677, 2809
NruI TCGCGA 1 cut(s) 290
NsiI ATGCAT 2 cut(s) 1312, 1792
NspI RCATGY 3 cut(s) 768, 2251, 2362
NspV TTCGAA 1 cut(s) 2541
PaeR7I CTCGAG 2 cut(s) 919, 2837
PciI ACATGT 2 cut(s) 764, 2247
PcsI WCGNNNNNNNCGW 1 cut(s) 2652
PctI GAATGC 2 cut(s) 345, 574
PdiI GCCGGC 1 cut(s) 3021
PdmI GAANNNNTTC 1 cut(s) 644
PfoI TCCNGGA 3 cut(s) 138, 410, 501
PleI GAGTC 7 cut(s) 267, 275, 1583, 1613, 2288, 2684, 2860
PpsI GAGTC 7 cut(s) 267, 275, 1583, 1613, 2288, 2684, 2860
PscI ACATGT 2 cut(s) 764, 2247
PshBI ATTAAT 1 cut(s) 1880
Psp124BI GAGCTC 3 cut(s) 243, 607, 2727
Psp6I CCWGG 6 cut(s) 138, 501, 824, 1687, 1708, 2827
PspEI GGTNACC 2 cut(s) 1200, 2369
PspFI CCCAGC 4 cut(s) 78, 165, 304, 1045
PspGI CCWGG 6 cut(s) 138, 501, 824, 1687, 1708, 2827
PspPI GGNCC 9 cut(s) 490, 658, 1391, 1823, 2041, 2324, 2923, 2928, 2975
PspXI VCTCGAGB 1 cut(s) 2837
PstI CTGCAG 3 cut(s) 396, 453, 477
PstNI CAGNNNCTG 1 cut(s) 472
PsuI RGATCY 5 cut(s) 142, 790, 1504, 1654, 1849
PvuII CAGCTG 1 cut(s) 472
RruI TCGCGA 1 cut(s) 290
RsaI GTAC 2 cut(s) 846, 2496
RsaNI GTAC 2 cut(s) 845, 2495
RseI CAYNNNNRTG 2 cut(s) 2406, 2860
SacI GAGCTC 3 cut(s) 243, 607, 2727
Sau96I GGNCC 9 cut(s) 490, 658, 1391, 1823, 2041, 2324, 2923, 2928, 2975
SchI GAGTC 7 cut(s) 267, 275, 1583, 1613, 2289, 2685, 2861
SduI GDGCHC 4 cut(s) 243, 607, 778, 2727
SfaNI GCATC 5 cut(s) 960, 1709, 1799, 2405, 2521
SfcI CTRYAG 3 cut(s) 392, 449, 473
Sfr274I CTCGAG 2 cut(s) 919, 2837
SfuI TTCGAA 1 cut(s) 2541
SinI GGWCC 6 cut(s) 490, 658, 1391, 2324, 2923, 2975
SlaI CTCGAG 2 cut(s) 919, 2837
SmiMI CAYNNNNRTG 2 cut(s) 2406, 2860
SmlI CTYRAG 5 cut(s) 919, 935, 1957, 2720, 2837
SmoI CTYRAG 5 cut(s) 919, 935, 1957, 2720, 2837
SpeI ACTAGT 1 cut(s) 2033
SstI GAGCTC 3 cut(s) 243, 607, 2727
TaaI ACNGT 7 cut(s) 221, 859, 1751, 2302, 2369, 2629, 3015
TaiI ACGT 3 cut(s) 1254, 2508, 2649
TaqII GACCGA 1 cut(s) 1338
TauI GCSGC 9 cut(s) 77, 207, 270, 542, 582, 596, 816, 901, 1828
TscAI CASTG 7 cut(s) 283, 864, 1500, 1656, 1767, 2017, 2496
TseFI GTSAC 6 cut(s) 278, 1319, 1762, 2230, 2677, 2809
Tsp45I GTSAC 6 cut(s) 278, 1319, 1762, 2230, 2677, 2809
TspDTI ATGAA 8 cut(s) 17, 942, 1356, 1463, 1751, 2151, 2256, 2941
TspGWI ACGGA 3 cut(s) 752, 2204, 2828
TspRI CASTG 7 cut(s) 283, 864, 1500, 1656, 1767, 2017, 2496
Vha464I CTTAAG 1 cut(s) 935
VpaK11BI GGWCC 6 cut(s) 490, 658, 1391, 2324, 2923, 2975
VspI ATTAAT 1 cut(s) 1880
XapI RAATTY 3 cut(s) 1450, 1683, 2293
XbaI TCTAGA 2 cut(s) 1435, 1852
XceI RCATGY 3 cut(s) 768, 2251, 2362
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XhoI CTCGAG 2 cut(s) 919, 2837
XmiI GTMKAC 1 cut(s) 1612
XmnI GAANNNNTTC 1 cut(s) 644
Zsp2I ATGCAT 2 cut(s) 1312, 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.