FvH4_5g31360
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015871
Physical Location & Seq
Forward (+)
22263852 .. 22267974
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31360.t1

Sequence Viewer

Length: 3420 bp
ATGGCCGCGCTGCTTCTCTCTCTTCTGGTGCTCTGCGCCGCACCGTTGCTCTCCTGCGCCGCCGCCCAGCGCGGCCCCGATAGGCAAGCCGAGATCGACGCTCTGACTACCTTCAAGCTCGACCTCCAGGATCCGATTGGCGCATTGAACGGCTGGGATGCCTCCACGCCTTCCGCCCCCTGCGACTGGCGCGGCGTCTTCTGCGACAATAGTTCTCGCGTCTCGGAGCTCCGTTTGCCTCGGCTTCAACTCGGCGGCCGACTCAGCGACTCGCTTTCCAAGCTCAAGATTCTGGGGAAGCTCAGTCTCCGGTCGAACTCCTTCAACGGCACTATCCCTGACTCACTCTCTCAATGCACGCGCCTACGCGCCGTCTTCTTGCAGAACAACTCTCTCTCCGGGAAGCTTCCGCCGGGGATCGGGAACCTCACTGCCTTGCAAATTCTCAATGTCGCAAGTAACCGCCTCTCCGGCGAAATCTCCGGCGAGCTACCTACGAGTCTTATTTACCTCGATCTCTCTTCCAACTCATTCTCCGGCGAGATTCCGAGGAGCATCGCGAACCTGACGCAGCTTCAGCTCATCAACCTGTCGTACAACCAGTTTTCCGGTCAGGTTCCGGCGAGCTTCGGCCAGCTCCAGAAGCTGCAGTTCCTCTGGCTGGACCATAACCTCTTGGAAGGGACGCTGCCGTCGGCGATAGCCAATTGCGCGGCGCTGGTGCATTTGAGCGTCGAAGGGAATGCGCTCGGCGGCGTGATTCCGGCGGCTATCGGAGCTCTGCCGAAGCTTCAGGTGCTGTCGCTGTCACAGAATAATTTCTCTGGTATGGTCCCATCCTCGTTGTTTTGCAATGCCTCATTCAATACGGCGTCGCTTAAGATTGTCCAGCTAGGGTTTAATTCGTTCACGGATATTGTTAAGCTTGAGACGGCCACATGTTTTAGTGGTCTGGTGGTTTTGGATCTTCAGCACAATCGGATTGGCGGCGATTTTCCTTGGTGGTTGACACAGGTGCCCAATTTGACAGTGTTGGATGTTTCTAGTAACTTATTTTCCGGCTTGGTGCCACCGGAGATTGGAAACCTTTCGAGGTTACAGGAGCTTAAGATGGCTAATAATTCATTTAGGGGTGCAATTCCGCAAGAGATTAAGCAATGCAGCTCACTAAGCGTGCTTGATCTCCAAGGAAATCAATTTTCGGGTGAAATTCCTGTGTTTTTGGGAGATTTGACAGGTTTGAAGGTGCTGTCTTTAGGGGAAAATCAGTTCTCGGGTTCCATTCCTCCGAGTATTGGTAAACTTTCCAGGCTGGAGACTTTGGGCTTGGGAGGGAATAACTTGACAGGAACTATGCCTGAGGAATTGTTAATAGGGTTGGGTAATTTGACCACACTGGACCTGAGTGGTAACAAGTTTTTTGGTGAAGTTGCCATCAGTATTGGGAGTTTGAGTCAGTTAATGGTGTTGAATTTAAGTGGCAATGGCTTTTCTGGAAGGGTTCCGACTAGCTTGGGTAGTCTTTTCAGGCTTACGACTATTGATCTGAGCAAACAAAACTTTTCTGGTGAGGTGCCCTCTGAGCTTTTGGGGTTACCTAATTTACAAGTCATTGCATTGCAAGAGAATAGGTTGTCTGGAGACATCCCTGAAGGTTTTAGCAGTTCGATGGGTCTGCATTATTTGAATCTCAGTTCAAATGCATTTTCTGGTCACATTCCAGAGAATTATGGTTTTCTTCGGTCATTGGTTGTTCTTTCATTGTCCAACAATCACATTTTAGGACCAATTCCACCGGAGCTTGGGAACTGCTCCGATATTCAAGTTCTGGAGCTGCAAATGAATTCTTTGACTGGCACCATTCCAGCCGATCTCTCTCGCTTATCAGTTTTGAAGGAACTTGATTTGGGTAGCAACAACTTAACAGGTGGAATCCCAGAGGAAATCTCCAAATGCTCATCATTAACTGCTTTGTTGTTGGACTCCAATCATCTCTCAGGCGGTATACCACACTCATTGTCTAAGCTATTGAACCTGACCACGCTGGATCTCTCCAATAACAATTTGAGTGGGCAAATTCCTGGTGATTTTGCATCTATTCCTGGCCTAGTGAACTTTGATGTCTCAATGAATAACCTCTATGGTGAGATTCCAGTAACACTTGGTTCTCGGTTCAACAATGCATCAGACTTTTCTGGGAATCAAAACTTATGTGGCCTGCCATTGGAAAAGAAGTGCAAGGATCTAGACAAGAAAGACAGAAATAAGAGGTTGATTCTGCTAATTGTGATTGTTGTCAGTGGAGCTTGCCTATTGTCTTTGTTGTGCTGCTTCTACATTTTCAGCCTCTTGAGGTGGCGCAAAAAGCTCCAACAGGGATCATCTGGGGAGAAGACTAGAAGTAGTGCAAGGGCAAGCTCAGGGACAAGTGAGGGCCGTGGAAGCTCTGATAGTGGAGTGCCAAAGCTTGTCATGTTCAACAGCAAAATCACACTTGCAGAGACAATTGCAGCCACCAGGCAATTTGATGAAGAAAATGTGCTGAGCAGAACACCATATGGCTTGGTTTTCAAGGCTTGTTATGAGGATGGTATGGTGATGTCCATCCGTCGGCTTCCTGATGCCACACTCAATGAAAACATATTCCGAAAAGAAGCAGAAGCACTAGGCAGAGTCAAGCACCGAAACTTAACAGTTCTTCGCGGCTACTATGCTGGTCCACCTGACCTCAGGCTTCTTATCTATGACTACATGCCCAACGGTAACCTAGCTACCCTTCTGCAAGAAGCATCACACCAAGACGGTCATGTCCTCAATTGGCCAATGCGCCATCTCATTGCACTTGGAATTGCTCGTGGCTTGGCTTTTCTACACACATCTACACTTGTACACGGCGATGTGAAGCCACAAAGCGTGCTATTCGATGCAGATTTTGAAGCCCATTTGTCTGATTTCGGCCTAGACAAACTAACATCAGCAACAAGCGCTACCACTCAAACCGAAGCCTCAACCTCAACCACAGTCGGCACATTGGGTTATGTCTCTCCAGAAGCAACACTAACAGGCAGAGTCACAAAGGAATCAGACGTTTACAGCTACGGGATCGTCTTGCTCGAGCTCCTAACAGGAAAGAAGCCAGTGATGTTCACAGAAGACGAAGACATAGTGAAGTGGGTGAAGAAGCAGCTTCAAAGAGGCCAAGTTGCCGAGCTACTAGAGCCAGGGTTGCTTGAGCTAGATCCTGAGTCATCCGAGTGGGAGGAGTTCTTGCTTGGAGTGAAAGTCGGGCTGCTTTGTACAGCACCGGACCCGCTGGATCGGCCCACCATGTCCGACATTGTTTTCATGCTCGAAGGCTGCCGGGTCGGACCTGACCTCCCCTCCTCCGCCGACCCGACATCGCTACCGTCGCCGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1140

Amino Acids

122.38

Weight (kDa)

5.34

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 69 3.9e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 167 - 226 1.2e-06 Leucine rich repeat
LRR_14 PF23598 168 - 270 7.6e-09 Leucine-rich repeat region
LRR_8 PF13855 317 - 375 2.9e-08 Leucine rich repeat
LRR_8 PF13855 340 - 400 2.5e-07 Leucine rich repeat
LRR_14 PF23598 341 - 466 1.7e-08 Leucine-rich repeat region
LRR_8 PF13855 389 - 448 2.5e-06 Leucine rich repeat
LRR_8 PF13855 413 - 473 1.4e-06 Leucine rich repeat
LRR_14 PF23598 432 - 544 2.7e-07 Leucine-rich repeat region
LRR_8 PF13855 533 - 593 3.1e-06 Leucine rich repeat
LRR_14 PF23598 534 - 709 1.5e-06 Leucine-rich repeat region
LRR_8 PF13855 608 - 665 1.1e-06 Leucine rich repeat
LRR_8 PF13855 654 - 713 2.2e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 847 - 1064 1.6e-30 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 853 - 1113 4.8e-31 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36180
fragaria_vesca FvH4_5g31360
prunus_persica Prupe.7G166100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0226621
rosa_laevigata RLG00000001713
rosa_multiflora Rmu_co8455755.1_g000001 Rmu_sc0002043.1_g000006 Rmu_sc0004294.1_g000002
rosa_roxburghii Rroxscaffold_3G00232650
rosa_rugosa Rorug07G0238700
rosa_samantha Rh7AG386200 Rh7BG372900 Rh7CG405500 Rh7DG384900
rosa_wichuraiana Rw7G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 691, 2807
AccB1I GGYRCC 4 cut(s) 1015, 1066, 1573, 1856
AccI GTMKAC 1 cut(s) 2005
AccII CGCG 9 cut(s) 8, 72, 192, 219, 361, 369, 560, 713, 2703
AcoI YGGCCR 5 cut(s) 3, 256, 631, 933, 2819
AcsI RAATTY 5 cut(s) 441, 1209, 1471, 1843, 2076
AcuI CTGAAG 4 cut(s) 560, 776, 953, 1671
AcyI GRCGYC 2 cut(s) 195, 872
AfaI GTAC 3 cut(s) 596, 2889, 3298
AfeI AGCGCT 1 cut(s) 2986
AfiI CCNNNNNNNGG 8 cut(s) 186, 419, 661, 1079, 1295, 1802, 2224, 2610
AflII CTTAAG 2 cut(s) 878, 1106
AflIII ACRYGT 1 cut(s) 938
AjnI CCWGG 6 cut(s) 126, 1307, 2080, 2101, 2516, 3220
AloI GAACNNNNNNTCC 2 cut(s) 1253, 1285
Alw21I GWGCWC 4 cut(s) 33, 231, 781, 3120
Alw26I GTCTC 8 cut(s) 226, 311, 923, 1310, 1635, 2128, 2495, 3046
AlwNI CAGNNNCTG 2 cut(s) 646, 799
Ama87I CYCGRG 2 cut(s) 1273, 3113
Aor51HI AGCGCT 1 cut(s) 2986
ApoI RAATTY 5 cut(s) 441, 1209, 1471, 1843, 2076
Asp700I GAANNNNTTC 3 cut(s) 320, 818, 1087
AsuC2I CCSGG 3 cut(s) 400, 414, 3362
AsuHPI GGTGA 7 cut(s) 1217, 1436, 1580, 2096, 2156, 2608, 3187
AvaI CYCGRG 2 cut(s) 1273, 3113
AvaII GGWCC 7 cut(s) 664, 832, 1399, 1784, 2717, 3307, 3368
AxyI CCTNAGG 2 cut(s) 1359, 2729
BaeGI GKGCMC 2 cut(s) 1020, 1578
BalI TGGCCA 1 cut(s) 2821
BamHI GGATCC 1 cut(s) 130
BanI GGYRCC 4 cut(s) 1015, 1066, 1573, 1856
BanII GRGCYC 3 cut(s) 231, 781, 3120
BauI CACGAG 1 cut(s) 2853
BbsI GAAGAC 5 cut(s) 190, 367, 2399, 3159, 3165
Bbv12I GWGCWC 4 cut(s) 33, 231, 781, 3120
BccI CCATC 7 cut(s) 844, 1105, 1442, 1663, 2582, 2612, 2838
BceAI ACGGC 8 cut(s) 166, 343, 356, 676, 885, 948, 2421, 2908
BcgI CGANNNNNNTGC 4 cut(s) 725, 759, 1657, 1691
BciT130I CCWGG 6 cut(s) 128, 1309, 2082, 2103, 2518, 3222
BcnI CCSGG 3 cut(s) 400, 414, 3362
BcoDI GTCTC 8 cut(s) 226, 311, 923, 1310, 1635, 2128, 2495, 3046
BfmI CTRYAG 1 cut(s) 647
BfoI RGCGCY 2 cut(s) 719, 2988
BfrI CTTAAG 2 cut(s) 878, 1106
BglI GCCNNNNNGGC 1 cut(s) 471
BlpI GCTNAGC 1 cut(s) 2543
Bme1390I CCNGG 9 cut(s) 128, 400, 414, 1309, 2082, 2103, 2518, 3222, 3362
Bme18I GGWCC 7 cut(s) 664, 832, 1399, 1784, 2717, 3307, 3368
BmeT110I CYCGRG 2 cut(s) 1273, 3113
BmrFI CCNGG 9 cut(s) 128, 400, 414, 1309, 2082, 2103, 2518, 3222, 3362
BmsI GCATC 7 cut(s) 148, 564, 2102, 2192, 2611, 2798, 2914
BpiI GAAGAC 5 cut(s) 190, 367, 2399, 3159, 3165
BplI GAGNNNNNCTC 4 cut(s) 1562, 1594, 1931, 1963
BpmI CTGGAG 6 cut(s) 110, 623, 1334, 1659, 1850, 3030
Bpu10I CCTNAGC 1 cut(s) 2419
Bpu1102I GCTNAGC 1 cut(s) 2543
BpuEI CTTGAG 4 cut(s) 269, 947, 2371, 3251
BpuMI CCSGG 3 cut(s) 400, 414, 3362
BsaBI GATNNNNATC 1 cut(s) 2603
BsaHI GRCGYC 2 cut(s) 195, 872
BsaJI CCNNGG 7 cut(s) 239, 413, 548, 998, 1186, 2437, 3221
BsaWI WCCGGW 5 cut(s) 309, 608, 1072, 1795, 3304
Bsc4I CCNNNNNNNGG 8 cut(s) 186, 419, 661, 1079, 1295, 1802, 2224, 2610
Bse118I RCCGGY 1 cut(s) 3412
Bse1I ACTGG 6 cut(s) 191, 601, 1401, 1858, 2153, 3137
Bse21I CCTNAGG 2 cut(s) 1359, 2729
Bse3DI GCAATG 6 cut(s) 859, 1163, 1489, 1611, 1616, 2835
Bse8I GATNNNNATC 1 cut(s) 2603
BseBI CCWGG 6 cut(s) 128, 1309, 2082, 2103, 2518, 3222
BseDI CCNNGG 7 cut(s) 239, 413, 548, 998, 1186, 2437, 3221
BseGI GGATG 7 cut(s) 163, 836, 1042, 1644, 2593, 2604, 3248
BseJI GATNNNNATC 1 cut(s) 2603
BseLI CCNNNNNNNGG 8 cut(s) 186, 419, 661, 1079, 1295, 1802, 2224, 2610
BseMI GCAATG 6 cut(s) 859, 1163, 1489, 1611, 1616, 2835
BseNI ACTGG 6 cut(s) 191, 601, 1401, 1858, 2153, 3137
BseRI GAGGAG 3 cut(s) 565, 3275, 3373
BseSI GKGCMC 2 cut(s) 1020, 1578
BseX3I CGGCCG 1 cut(s) 256
BseYI CCCAGC 2 cut(s) 66, 153
Bsh1236I CGCG 9 cut(s) 8, 72, 192, 219, 361, 369, 560, 713, 2703
Bsh1285I CGRYCG 2 cut(s) 259, 314
BshNI GGYRCC 4 cut(s) 1015, 1066, 1573, 1856
BsiEI CGRYCG 2 cut(s) 259, 314
BsiHKAI GWGCWC 4 cut(s) 33, 231, 781, 3120
BsiHKCI CYCGRG 2 cut(s) 1273, 3113
BslFI GGGAC 3 cut(s) 697, 818, 2437
BslI CCNNNNNNNGG 8 cut(s) 186, 419, 661, 1079, 1295, 1802, 2224, 2610
BsmAI GTCTC 8 cut(s) 226, 311, 923, 1310, 1635, 2128, 2495, 3046
BsmBI CGTCTC 2 cut(s) 226, 923
BsmFI GGGAC 3 cut(s) 697, 818, 2437
BsmI GAATGC 1 cut(s) 748
BsoBI CYCGRG 2 cut(s) 1273, 3113
Bsp1286I GDGCHC 6 cut(s) 33, 231, 781, 1020, 1578, 3120
Bsp1407I TGTACA 2 cut(s) 2887, 3296
Bsp1720I GCTNAGC 1 cut(s) 2543
Bsp68I TCGCGA 1 cut(s) 560
BspFNI CGCG 9 cut(s) 8, 72, 192, 219, 361, 369, 560, 713, 2703
BspMAI CTGCAG 1 cut(s) 651
BspT107I GGYRCC 4 cut(s) 1015, 1066, 1573, 1856
BspTI CTTAAG 2 cut(s) 878, 1106
BsrDI GCAATG 6 cut(s) 859, 1163, 1489, 1611, 1616, 2835
BsrFI RCCGGY 1 cut(s) 3412
BsrGI TGTACA 2 cut(s) 2887, 3296
BsrI ACTGG 6 cut(s) 191, 601, 1401, 1858, 2153, 3137
BssAI RCCGGY 1 cut(s) 3412
BssECI CCNNGG 7 cut(s) 239, 413, 548, 998, 1186, 2437, 3221
BssNAI GTATAC 1 cut(s) 2006
BssNI GRCGYC 2 cut(s) 195, 872
BssSI CACGAG 1 cut(s) 2853
BssT1I CCWWGG 2 cut(s) 998, 1186
Bst1107I GTATAC 1 cut(s) 2006
Bst2BI CACGAG 1 cut(s) 2853
Bst2UI CCWGG 6 cut(s) 128, 1309, 2082, 2103, 2518, 3222
Bst4CI ACNGT 7 cut(s) 45, 1030, 2695, 2762, 2804, 3022, 3408
Bst6I CTCTTC 2 cut(s) 27, 526
BstACI GRCGYC 2 cut(s) 195, 872
BstAFI CTTAAG 2 cut(s) 878, 1106
BstAUI TGTACA 2 cut(s) 2887, 3296
BstDSI CCRYGG 1 cut(s) 2437
BstEII GGTNACC 2 cut(s) 1593, 2762
BstF5I GGATG 7 cut(s) 163, 836, 1042, 1644, 2593, 2604, 3248
BstFNI CGCG 9 cut(s) 8, 72, 192, 219, 361, 369, 560, 713, 2703
BstH2I RGCGCY 2 cut(s) 719, 2988
BstMAI GTCTC 8 cut(s) 226, 311, 923, 1310, 1635, 2128, 2495, 3046
BstMCI CGRYCG 2 cut(s) 259, 314
BstNI CCWGG 6 cut(s) 128, 1309, 2082, 2103, 2518, 3222
BstNSI RCATGY 2 cut(s) 942, 2755
BstPI GGTNACC 2 cut(s) 1593, 2762
BstSCI CCNGG 9 cut(s) 126, 398, 412, 1307, 2080, 2101, 2516, 3220, 3360
BstSFI CTRYAG 1 cut(s) 647
BstSLI GKGCMC 2 cut(s) 1020, 1578
BstUI CGCG 9 cut(s) 8, 72, 192, 219, 361, 369, 560, 713, 2703
BstV2I GAAGAC 5 cut(s) 190, 367, 2399, 3159, 3165
BstX2I RGATCY 5 cut(s) 130, 964, 2047, 2242, 3238
BstYI RGATCY 5 cut(s) 130, 964, 2047, 2242, 3238
BstZ17I GTATAC 1 cut(s) 2006
BstZI CGGCCG 1 cut(s) 256
Bsu36I CCTNAGG 2 cut(s) 1359, 2729
BtgI CCRYGG 1 cut(s) 2437
BtgZI GCGATG 3 cut(s) 541, 2910, 3384
BtsCI GGATG 7 cut(s) 163, 836, 1042, 1644, 2593, 2604, 3248
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 5 cut(s) 429, 1035, 1394, 2305, 3144
BtuMI TCGCGA 1 cut(s) 560
CaiI CAGNNNCTG 2 cut(s) 646, 799
Cfr10I RCCGGY 1 cut(s) 3412
CseI GACGC 7 cut(s) 107, 184, 208, 577, 694, 721, 861
Csp6I GTAC 3 cut(s) 595, 2888, 3297
CspCI CAANNNNNGTGG 4 cut(s) 1998, 2033, 2050, 2085
CviAII CATG 6 cut(s) 939, 2473, 2752, 2807, 3328, 3346
CviQI GTAC 3 cut(s) 595, 2888, 3297
DrdI GACNNNNNNGTC 2 cut(s) 691, 2807
DseDI GACNNNNNNGTC 2 cut(s) 691, 2807
EaeI YGGCCR 5 cut(s) 3, 256, 631, 933, 2819
EagI CGGCCG 1 cut(s) 256
Eam1104I CTCTTC 2 cut(s) 27, 526
EarI CTCTTC 2 cut(s) 27, 526
EciI GGCGGA 3 cut(s) 163, 399, 3376
Ecl136II GAGCTC 3 cut(s) 229, 779, 3118
EclXI CGGCCG 1 cut(s) 256
Eco130I CCWWGG 2 cut(s) 998, 1186
Eco24I GRGCYC 3 cut(s) 231, 781, 3120
Eco47I GGWCC 7 cut(s) 664, 832, 1399, 1784, 2717, 3307, 3368
Eco47III AGCGCT 1 cut(s) 2986
Eco52I CGGCCG 1 cut(s) 256
Eco53kI GAGCTC 3 cut(s) 229, 779, 3118
Eco57I CTGAAG 4 cut(s) 560, 776, 953, 1671
Eco81I CCTNAGG 2 cut(s) 1359, 2729
Eco88I CYCGRG 2 cut(s) 1273, 3113
Eco91I GGTNACC 2 cut(s) 1593, 2762
EcoICRI GAGCTC 3 cut(s) 229, 779, 3118
EcoO65I GGTNACC 2 cut(s) 1593, 2762
EcoRI GAATTC 1 cut(s) 1843
EcoRII CCWGG 6 cut(s) 126, 1307, 2080, 2101, 2516, 3220
EcoT14I CCWWGG 2 cut(s) 998, 1186
EcoT22I ATGCAT 2 cut(s) 1705, 2185
EcoT38I GRGCYC 3 cut(s) 231, 781, 3120
ErhI CCWWGG 2 cut(s) 998, 1186
Esp3I CGTCTC 2 cut(s) 226, 923
FaeI CATG 6 cut(s) 942, 2476, 2755, 2810, 3331, 3349
FaqI GGGAC 3 cut(s) 697, 818, 2437
FatI CATG 6 cut(s) 938, 2472, 2751, 2806, 3327, 3345
FauI CCCGC 1 cut(s) 3318
FauNDI CATATG 1 cut(s) 2557
FblI GTMKAC 1 cut(s) 2005
FokI GGATG 7 cut(s) 170, 823, 1049, 1631, 2591, 2600, 3235
FriOI GRGCYC 3 cut(s) 231, 781, 3120
GsaI CCCAGC 2 cut(s) 70, 157
GsuI CTGGAG 6 cut(s) 110, 623, 1334, 1659, 1850, 3030
HaeII RGCGCY 2 cut(s) 719, 2988
HgaI GACGC 7 cut(s) 107, 184, 208, 577, 694, 721, 861
Hin1I GRCGYC 2 cut(s) 195, 872
Hin1II CATG 6 cut(s) 942, 2476, 2755, 2810, 3331, 3349
HincII GTYRAC 1 cut(s) 1008
HindII GTYRAC 1 cut(s) 1008
HindIII AAGCTT 4 cut(s) 404, 788, 923, 2465
HphI GGTGA 7 cut(s) 1217, 1436, 1580, 2096, 2156, 2608, 3187
Hpy166II GTNNAC 9 cut(s) 909, 1008, 1301, 2006, 2113, 2720, 2890, 3091, 3147
Hpy8I GTNNAC 9 cut(s) 909, 1008, 1301, 2006, 2113, 2720, 2890, 3091, 3147
Hpy99I CGWCG 6 cut(s) 101, 697, 737, 877, 2613, 3412
HpyCH4III ACNGT 7 cut(s) 45, 1030, 2695, 2762, 2804, 3022, 3408
HpyCH4IV ACGT 1 cut(s) 3087
HpySE526I ACGT 1 cut(s) 3087
Hsp92I GRCGYC 2 cut(s) 195, 872
Hsp92II CATG 6 cut(s) 942, 2476, 2755, 2810, 3331, 3349
LweI GCATC 7 cut(s) 148, 564, 2102, 2192, 2611, 2798, 2914
MaeII ACGT 1 cut(s) 3087
MfeI CAATTG 3 cut(s) 706, 2505, 2815
MflI RGATCY 5 cut(s) 130, 964, 2047, 2242, 3238
MhlI GDGCHC 6 cut(s) 33, 231, 781, 1020, 1578, 3120
MlsI TGGCCA 1 cut(s) 2821
MluNI TGGCCA 1 cut(s) 2821
MlyI GAGTC 9 cut(s) 255, 263, 335, 508, 1462, 1976, 2682, 3078, 3254
MmeI TCCRAC 8 cut(s) 549, 1014, 1529, 1791, 1959, 2395, 3346, 3357
Mox20I TGGCCA 1 cut(s) 2821
Mph1103I ATGCAT 2 cut(s) 1705, 2185
MroXI GAANNNNTTC 3 cut(s) 320, 818, 1087
MscI TGGCCA 1 cut(s) 2821
MslI CAYNNNNRTG 2 cut(s) 2895, 3253
Msp20I TGGCCA 1 cut(s) 2821
MspA1I CMGCKG 1 cut(s) 3313
MspCI CTTAAG 2 cut(s) 878, 1106
MspR9I CCNGG 9 cut(s) 128, 400, 414, 1309, 2082, 2103, 2518, 3222, 3362
MteI GCGCNGCGC 1 cut(s) 714
MunI CAATTG 3 cut(s) 706, 2505, 2815
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 6 cut(s) 128, 1309, 2082, 2103, 2518, 3222
MvnI CGCG 9 cut(s) 8, 72, 192, 219, 361, 369, 560, 713, 2703
NciI CCSGG 3 cut(s) 400, 414, 3362
NdeI CATATG 1 cut(s) 2557
NlaIII CATG 6 cut(s) 942, 2476, 2755, 2810, 3331, 3349
NmeAIII GCCGAG 5 cut(s) 115, 220, 231, 729, 3232
NmuCI GTSAC 3 cut(s) 807, 1712, 3070
NruI TCGCGA 1 cut(s) 560
NsiI ATGCAT 2 cut(s) 1705, 2185
NspI RCATGY 2 cut(s) 942, 2755
PaeR7I CTCGAG 1 cut(s) 3113
PciI ACATGT 1 cut(s) 938
PcsI WCGNNNNNNNCGW 1 cut(s) 3111
PctI GAATGC 1 cut(s) 748
PdmI GAANNNNTTC 3 cut(s) 320, 818, 1087
PfeI GAWTC 9 cut(s) 289, 544, 760, 1687, 1932, 2149, 2200, 2275, 3080
PfoI TCCNGGA 2 cut(s) 126, 398
PleI GAGTC 9 cut(s) 255, 263, 335, 507, 1461, 1976, 2681, 3077, 3253
PpsI GAGTC 9 cut(s) 255, 263, 335, 507, 1461, 1976, 2681, 3077, 3253
PscI ACATGT 1 cut(s) 938
Psp124BI GAGCTC 3 cut(s) 231, 781, 3120
Psp6I CCWGG 6 cut(s) 126, 1307, 2080, 2101, 2516, 3220
PspEI GGTNACC 2 cut(s) 1593, 2762
PspFI CCCAGC 2 cut(s) 66, 153
PspGI CCWGG 6 cut(s) 126, 1307, 2080, 2101, 2516, 3220
PspXI VCTCGAGB 1 cut(s) 3113
PstI CTGCAG 1 cut(s) 651
PstNI CAGNNNCTG 2 cut(s) 646, 799
PsuI RGATCY 5 cut(s) 130, 964, 2047, 2242, 3238
RruI TCGCGA 1 cut(s) 560
RsaI GTAC 3 cut(s) 596, 2889, 3298
RsaNI GTAC 3 cut(s) 595, 2888, 3297
RseI CAYNNNNRTG 2 cut(s) 2895, 3253
SacI GAGCTC 3 cut(s) 231, 781, 3120
SchI GAGTC 9 cut(s) 255, 263, 335, 508, 1462, 1976, 2682, 3078, 3254
ScrFI CCNGG 9 cut(s) 128, 400, 414, 1309, 2082, 2103, 2518, 3222, 3362
SduI GDGCHC 6 cut(s) 33, 231, 781, 1020, 1578, 3120
SfaNI GCATC 7 cut(s) 148, 564, 2102, 2192, 2611, 2798, 2914
SfcI CTRYAG 1 cut(s) 647
Sfr274I CTCGAG 1 cut(s) 3113
SgrAI CRCCGGYG 1 cut(s) 3412
SinI GGWCC 7 cut(s) 664, 832, 1399, 1784, 2717, 3307, 3368
SlaI CTCGAG 1 cut(s) 3113
SmiMI CAYNNNNRTG 2 cut(s) 2895, 3253
SmlI CTYRAG 7 cut(s) 284, 878, 926, 1106, 2350, 3113, 3230
SmoI CTYRAG 7 cut(s) 284, 878, 926, 1106, 2350, 3113, 3230
SstI GAGCTC 3 cut(s) 231, 781, 3120
StyD4I CCNGG 9 cut(s) 126, 398, 412, 1307, 2080, 2101, 2516, 3220, 3360
StyI CCWWGG 2 cut(s) 998, 1186
TaaI ACNGT 7 cut(s) 45, 1030, 2695, 2762, 2804, 3022, 3408
TaiI ACGT 1 cut(s) 3090
TaqII GACCGA 1 cut(s) 1731
TatI WGTACW 2 cut(s) 2887, 3296
TfiI GAWTC 9 cut(s) 289, 544, 760, 1687, 1932, 2149, 2200, 2275, 3080
TscAI CASTG 5 cut(s) 436, 1035, 1401, 2305, 3144
TseFI GTSAC 3 cut(s) 807, 1712, 3070
Tsp45I GTSAC 3 cut(s) 807, 1712, 3070
TspDTI ATGAA 7 cut(s) 1113, 1749, 1856, 2144, 2544, 2649, 3334
TspGWI ACGGA 3 cut(s) 221, 926, 2597
TspRI CASTG 5 cut(s) 436, 1035, 1401, 2305, 3144
Vha464I CTTAAG 2 cut(s) 878, 1106
VpaK11BI GGWCC 7 cut(s) 664, 832, 1399, 1784, 2717, 3307, 3368
XapI RAATTY 5 cut(s) 441, 1209, 1471, 1843, 2076
XbaI TCTAGA 1 cut(s) 2245
XceI RCATGY 2 cut(s) 942, 2755
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XhoI CTCGAG 1 cut(s) 3113
XmiI GTMKAC 1 cut(s) 2005
XmnI GAANNNNTTC 3 cut(s) 320, 818, 1087
Zsp2I ATGCAT 2 cut(s) 1705, 2185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.