RchiOBHm_Chr7g0226621
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0015871
Physical Location & Seq
Forward (+)
49888564 .. 49892697
4134 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20297

Sequence Viewer

Length: 3441 bp
ATGAAACCCATTATGCCTGCGCTTCTTCTTCCTCTCTTTCTTCTGGTGCTCTGCGGCGCACCCTTCTTCTCCTGCGCCGCCGCGCAGCGCAGCCCCGAAAGCCTAGCCGAGATCGACGCCCTCACCACCTTCAGGCTCGACCTCCAGGATCCGATTGGCGCATTGAACGGCTGGGATTCCTCCACGCCTTCCGCTCCCTGCGACTGGCGCGGCGTCTTTTGCGACAGTAGTAGTCGGGTCTCCGAGCTCCGCTTGCCTCGCCTCCAACTCGGCGGCCGACTCAGTGACTCGCTCGCCAAGCTCACCATGCTGGGGAAGCTCAGCCTCCGGTCAAACTCCTTCAACGGGAGCATTCCTGACTCGCTTTCCCAATGCACGCGCCTACGCGCCGTCTTCCTGCAGAACAACTCTCTCTCCGGGAAGCTGCCCCCGGAGATCGGGAACCTGACCGGGCTGCAGATTCTCAACGTCGCCGGTAACCGCCTCTCCGGCGAAATCTCCGGCGAGCTCCCTCCGAGCCTCGTGTACCTCGATCTCTCCTCGAACGATTTCTCCGGCGAGGTTCCGAGGAGCATCGCGAACCTGACTCAGCTTCAGCTCATCAACCTGTCGTACAACCAGTTTTCCGGTCAGGTTCCGGCGAGCTTGGGCCAGCTCCGGCAGCTGCAGTTCCTCTGGCTGGACCACAACCTCCTGGAAGGGACGCTGCCGTCGGCGATAGCCAATTGTGCGGCGCTGGTGCATTTCAGCGTCGAAGGGAATGCGCTCGGCGGCGTGATTCCGGCGGCTGTCGGAGCTCTGCCGAAGCTTCAGGTGCTGTCGCTTTCACAGAATAATTTCTCTGGTATGGTCCCATCCTCGTTGTTTTGCAATGCCTCATTCAATACGGCGTCGCTTAGGATTGTCCAGCTAGGGTTTAATTCGTTCACGGATATAGTTAAGCCTGAGACAGCCACATGTTTTAGTGCTCTGGTGGTTTTGGATCTTCAGCACAATCAGATTGGCGGCGATTTTCCCTGGTGGATAATAACACAGGTACCAAACTTGACAGTGTTGGATGTTTCTAGTAACTCATTCTCCGGCGTGGTGCCGCCGGGGATTGGAAACCTCTCCAGGTTACAGGAGCTTAAGATGGCTAATAATTCATTTAGCGGTCCGATTCCGCAAGAGATTAAGCAATGCAGCTCACTAAGCGTGCTTGATCTCCAAGGAAATCGATTTTCGGGGGAAATTCCTGTGTTTCTGGGGGATTTGACAGGTTTGAAGGTGCTATCTTTAGGGGAAAATCAGTTCTCGGGTTCCATTCCTCCGAGTTTTGGTAATCTTACGAGGCTGGAGACTTTGGGATTGGGAGGGAACAACTTGACCGGAGCTATGCCTGAGGAGTTGTTGATAGGGTTGGGTAATTTGACAACATTGGACCTCAGTGGGAACAGGTTTTTTGGTGAAGTTGCCATCAGCATTGGGAGTTTGAGTCAGTTAATGGTGCTGAATCTGAGTGGCAATGGCTTTTCTGGGAGGGTGCCGACTAGCTTGGGTAGTCTTTTCAGGCTTACTACTATTGATCTGAGCAAACAAAACTTCTCTGGTGAGGTGCCTTCTGAGCTTTTGGGGTTACCCAATTTACAAGTCATTGCATTGCAAGAGAACAGGTTGTCTGGAGACGTTCCTGAAGGTTTTAGTAGCTTGATGGGTCTGCATTATTTGAACCTCAGTTCAAATGCATTTTCTGGTCACATTCCGGAGAATTATGGTTTTCTTCGGTCATTGGTTGTTCTTTCATTGTCCAACAATCACATTTCAGGACCAATTCCACCCGAGCTTGGAAACTGCTCTGATCTTCAAGTTCTAGAGCTGCAAATGAATTCCTTGACTGGCACCATTCCAGCCGATCTCTCTCGCCTAACAGTGTTAAAGGATCTTGATTTGGGTAGCAACAACTTAACAGGTAGAATCCCAGAGGAAATGTCTAAATGCTCATCATTAACTGCTTTGTTGTTGGACTCCAATCATCTCTCAGGTGGTATACCAGACTCGTTGTCTAAGCTATTGAACCTAACTACACTGGATCTCTCCAATAACAATTTGAGCGGGCAAATTCCTGGTGATCTTGCATCTATTCCTGGCCTAGTTAACTTCAATGTCTCAATGAATAACCTCGACGGTGAGATTCCAGTGACGCTTGGTTCTCGGTTCAACAATGCATCAGACTTTTCAGGGAATCAAAACTTATGTGGGCCGCCATTGGAAAAGAAGTGCAAGGATCTAGACAAGCAAGATAGAAATAAGAGACTAATTCTTTTGATTGTGATTGTTGTTAGTGGAGCTTGCCTATTAGCTTTGTGCTGCTGCTTCTACATTTTCAGCCTCTTGAGATGGCGCAAAAAGCTCAAACAGGGATCATCTGGAGAGAAGACTAGAAGCAGTGCGAGGGCAAGCTCAGGAACTAGTGAGGGCCGTGGAAGCTCTGATAATGGAGTGCCAAAGCTTGTCATGTTCAACAGCAAAATCACACTTGCAGAGACAATTGCAGCCACAAGGCAATTCGACGAAGAAAATGTGCTGAGCAGATCACCATATGGCTTGGTTTTCAAGGCTAGTTATGAGGACGGTATGGTGATGTCCATCCGTCGCCTCCCCGATGCAACACTCGATGAAAACATGTTCCGAAAAGAAGCAGAATCACTAGGCAGAGTCAAGCACCGGAATTTAACAGTTCTTCGAGGCTACTATGCCGGTCCACCTGACCTCAGGCTTCTAATCTATGACTACATGCCCAACGGTAACCTAGCCACCCTTCTGCAAGAAGCATCACACCAAGACGGCCATGTCCTCAATTGGCCAATGCGCCATCTCATTGCACTTGGAATCGCTCGTGGCTTGGCTTTTCTACACACATCTTCACTCGTACACGGCGACGTGAAGCCACAAAGCGTGCTATTCGATGCAGATTTCGAAGCCCATTTGTCTGATTTCGGCCTAGACAAACTCACATCAGCAACCAGCACTACTACTCAAACCGAAGCCTCAACCTCAACCACCGTCGGGACATTAGGCTACGTCTCTCACGAAGCAACACTAACAGGCAGAGTCACAAAGGAATCCGATGTTTACAGCTACGGGATCGTCTTGCTCGAGCTCTTAACAGGAAAGAGGCCGGTGATGTTCACCGAAGACGAAGACATAGTGAAGTGGGTGAAGAAGCAGCTTCAAAGAGGCCAAGTCACGGAGCTACTAGAGCCAGGACTGCTCGAGCTTGACCCCGAGTCATCCGAGTGGGAAGAGTTCTTGCTTGGCGTGAAAGTCGGGTTGCTTTGCACGGCACCCGACCCGCTGGACCGGCCCACCATGTCCGACATTGTTTTCATGCTCGAAGGCTGCCGGGTCGGACCTGATCTCCCGTCCTCCGCCGACCCGACAACGCAACCGTCGCCGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009791 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048731 GO:0048856 GO:0071944 GO:0090696 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1146

Amino Acids

123.29

Weight (kDa)

5.15

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 75 1.4e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 172 - 232 1.1e-07 Leucine rich repeat
LRR_14 PF23598 174 - 354 3.5e-09 Leucine-rich repeat region
LRR_14 PF23598 320 - 472 3.2e-09 Leucine-rich repeat region
LRR_8 PF13855 347 - 407 8.3e-08 Leucine rich repeat
LRR_14 PF23598 439 - 548 1.4e-06 Leucine-rich repeat region
LRR_14 PF23598 497 - 739 2.2e-12 Leucine-rich repeat region
LRR_8 PF13855 517 - 575 5.1e-07 Leucine rich repeat
LRR_8 PF13855 540 - 600 2.1e-07 Leucine rich repeat
LRR_8 PF13855 614 - 672 8.7e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 853 - 1071 8.9e-30 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 860 - 1120 2.6e-29 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015871)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36180
fragaria_vesca FvH4_5g31360
prunus_persica Prupe.7G166100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0226621
rosa_laevigata RLG00000001713
rosa_multiflora Rmu_co8455755.1_g000001 Rmu_sc0002043.1_g000006 Rmu_sc0004294.1_g000002
rosa_roxburghii Rroxscaffold_3G00232650
rosa_rugosa Rorug07G0238700
rosa_samantha Rh7AG386200 Rh7BG372900 Rh7CG405500 Rh7DG384900
rosa_wichuraiana Rw7G032420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 709, 2828
Acc65I GGTACC 1 cut(s) 1036
AccB1I GGYRCC 6 cut(s) 1036, 1087, 1522, 1594, 1877, 3322
AccBSI CCGCTC 2 cut(s) 194, 2091
AccI GTMKAC 1 cut(s) 2026
AccII CGCG 5 cut(s) 83, 210, 379, 387, 578
AccIII TCCGGA 1 cut(s) 1741
AclWI GGATC 8 cut(s) 143, 156, 990, 1926, 2076, 2271, 2407, 3131
AcoI YGGCCR 3 cut(s) 274, 2824, 2840
AcsI RAATTY 4 cut(s) 1230, 1864, 2097, 2707
AcuI CTGAAG 5 cut(s) 115, 578, 794, 971, 1692
AcyI GRCGYC 3 cut(s) 117, 213, 890
AfaI GTAC 4 cut(s) 527, 614, 1038, 2910
AflII CTTAAG 1 cut(s) 1127
AflIII ACRYGT 2 cut(s) 956, 2661
AhdI GACNNNNNGTC 2 cut(s) 2038, 3265
AhlI ACTAGT 1 cut(s) 2447
AjiI CACGTC 1 cut(s) 2920
AjnI CCWGG 7 cut(s) 144, 693, 1016, 1112, 2101, 2122, 3241
AloI GAACNNNNNNTCC 4 cut(s) 536, 568, 1274, 1306
Alw21I GWGCWC 6 cut(s) 51, 249, 510, 799, 970, 3141
Alw26I GTCTC 8 cut(s) 244, 941, 1331, 1656, 2149, 2284, 2516, 3067
AlwI GGATC 8 cut(s) 143, 156, 990, 1926, 2076, 2271, 2407, 3131
AlwNI CAGNNNCTG 1 cut(s) 817
Ama87I CYCGRG 5 cut(s) 1294, 1817, 3134, 3251, 3263
Aor13HI TCCGGA 1 cut(s) 1741
ApoI RAATTY 4 cut(s) 1230, 1864, 2097, 2707
Asp700I GAANNNNTTC 2 cut(s) 548, 836
Asp718I GGTACC 1 cut(s) 1036
AsuC2I CCSGG 5 cut(s) 418, 431, 451, 1095, 3383
AsuII TTCGAA 1 cut(s) 2955
AvaI CYCGRG 5 cut(s) 1294, 1817, 3134, 3251, 3263
AvaII GGWCC 8 cut(s) 682, 850, 1154, 1420, 1805, 2738, 3337, 3389
AxyI CCTNAGG 2 cut(s) 1380, 2750
BaeI ACNNNNGTAYC 2 cut(s) 1020, 1053
BalI TGGCCA 1 cut(s) 2842
BamHI GGATCC 1 cut(s) 148
BanI GGYRCC 6 cut(s) 1036, 1087, 1522, 1594, 1877, 3322
BanII GRGCYC 4 cut(s) 249, 510, 799, 3141
BauI CACGAG 2 cut(s) 521, 2874
BbsI GAAGAC 4 cut(s) 385, 2420, 3180, 3186
Bbv12I GWGCWC 6 cut(s) 51, 249, 510, 799, 970, 3141
BccI CCATC 7 cut(s) 862, 1126, 1463, 1684, 2370, 2633, 2859
BceAI ACGGC 8 cut(s) 184, 374, 694, 903, 2442, 2839, 2929, 3336
BcgI CGANNNNNNTGC 2 cut(s) 743, 777
BciT130I CCWGG 7 cut(s) 146, 695, 1018, 1114, 2103, 2124, 3243
BcnI CCSGG 5 cut(s) 418, 431, 451, 1095, 3383
BcoDI GTCTC 8 cut(s) 244, 941, 1331, 1656, 2149, 2284, 2516, 3067
BcuI ACTAGT 1 cut(s) 2447
BfmI CTRYAG 3 cut(s) 398, 455, 665
BfoI RGCGCY 1 cut(s) 737
BfrI CTTAAG 1 cut(s) 1127
BglI GCCNNNNNGGC 1 cut(s) 489
BlpI GCTNAGC 2 cut(s) 320, 2564
Bme18I GGWCC 8 cut(s) 682, 850, 1154, 1420, 1805, 2738, 3337, 3389
BmeRI GACNNNNNGTC 2 cut(s) 2038, 3265
BmeT110I CYCGRG 5 cut(s) 1294, 1817, 3134, 3251, 3263
BmgBI CACGTC 1 cut(s) 2920
BmsI GCATC 6 cut(s) 582, 2123, 2213, 2632, 2819, 2935
BpiI GAAGAC 4 cut(s) 385, 2420, 3180, 3186
BpmI CTGGAG 5 cut(s) 128, 1096, 1355, 1680, 2427
Bpu10I CCTNAGC 2 cut(s) 896, 2440
Bpu1102I GCTNAGC 2 cut(s) 320, 2564
Bpu14I TTCGAA 1 cut(s) 2955
BpuEI CTTGAG 1 cut(s) 2392
BpuMI CCSGG 5 cut(s) 418, 431, 451, 1095, 3383
Bsa29I ATCGAT 1 cut(s) 1216
BsaBI GATNNNNATC 1 cut(s) 2624
BsaHI GRCGYC 3 cut(s) 117, 213, 890
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 6 cut(s) 429, 566, 1016, 1094, 1207, 2458
BsaWI WCCGGW 5 cut(s) 327, 626, 1367, 1741, 2703
Bse118I RCCGGY 5 cut(s) 473, 2735, 3157, 3339, 3433
Bse1I ACTGG 5 cut(s) 209, 619, 1879, 2070, 2174
Bse21I CCTNAGG 2 cut(s) 1380, 2750
Bse3DI GCAATG 6 cut(s) 877, 1184, 1510, 1632, 1637, 2856
Bse8I GATNNNNATC 1 cut(s) 2624
BseAI TCCGGA 1 cut(s) 1741
BseBI CCWGG 7 cut(s) 146, 695, 1018, 1114, 2103, 2124, 3243
BseCI ATCGAT 1 cut(s) 1216
BseDI CCNNGG 6 cut(s) 429, 566, 1016, 1094, 1207, 2458
BseGI GGATG 4 cut(s) 854, 1063, 2625, 3269
BseJI GATNNNNATC 1 cut(s) 2624
BseMI GCAATG 6 cut(s) 877, 1184, 1510, 1632, 1637, 2856
BseNI ACTGG 5 cut(s) 209, 619, 1879, 2070, 2174
BseRI GAGGAG 3 cut(s) 529, 583, 1397
BseX3I CGGCCG 1 cut(s) 274
BseYI CCCAGC 2 cut(s) 171, 310
Bsh1236I CGCG 5 cut(s) 83, 210, 379, 387, 578
Bsh1285I CGRYCG 1 cut(s) 277
BshNI GGYRCC 6 cut(s) 1036, 1087, 1522, 1594, 1877, 3322
BshVI ATCGAT 1 cut(s) 1216
BsiEI CGRYCG 1 cut(s) 277
BsiHKAI GWGCWC 6 cut(s) 51, 249, 510, 799, 970, 3141
BsiHKCI CYCGRG 5 cut(s) 1294, 1817, 3134, 3251, 3263
BslFI GGGAC 3 cut(s) 715, 836, 3061
BsmAI GTCTC 8 cut(s) 244, 941, 1331, 1656, 2149, 2284, 2516, 3067
BsmBI CGTCTC 2 cut(s) 1656, 3067
BsmFI GGGAC 3 cut(s) 715, 836, 3061
BsmI GAATGC 2 cut(s) 351, 766
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 5 cut(s) 1294, 1817, 3134, 3251, 3263
Bsp119I TTCGAA 1 cut(s) 2955
Bsp1286I GDGCHC 6 cut(s) 51, 249, 510, 799, 970, 3141
Bsp13I TCCGGA 1 cut(s) 1741
Bsp1720I GCTNAGC 2 cut(s) 320, 2564
Bsp68I TCGCGA 1 cut(s) 578
BspDI ATCGAT 1 cut(s) 1216
BspEI TCCGGA 1 cut(s) 1741
BspFNI CGCG 5 cut(s) 83, 210, 379, 387, 578
BspMAI CTGCAG 3 cut(s) 402, 459, 669
BspPI GGATC 8 cut(s) 143, 156, 990, 1926, 2076, 2271, 2407, 3131
BspT104I TTCGAA 1 cut(s) 2955
BspT107I GGYRCC 6 cut(s) 1036, 1087, 1522, 1594, 1877, 3322
BspTI CTTAAG 1 cut(s) 1127
BspTNI GGTCTC 1 cut(s) 244
BsrBI CCGCTC 2 cut(s) 194, 2091
BsrDI GCAATG 6 cut(s) 877, 1184, 1510, 1632, 1637, 2856
BsrFI RCCGGY 5 cut(s) 473, 2735, 3157, 3339, 3433
BsrI ACTGG 5 cut(s) 209, 619, 1879, 2070, 2174
BssAI RCCGGY 5 cut(s) 473, 2735, 3157, 3339, 3433
BssECI CCNNGG 6 cut(s) 429, 566, 1016, 1094, 1207, 2458
BssNAI GTATAC 1 cut(s) 2027
BssNI GRCGYC 3 cut(s) 117, 213, 890
BssSI CACGAG 2 cut(s) 521, 2874
BssT1I CCWWGG 1 cut(s) 1207
Bst1107I GTATAC 1 cut(s) 2027
Bst2BI CACGAG 2 cut(s) 521, 2874
Bst2UI CCWGG 7 cut(s) 146, 695, 1018, 1114, 2103, 2124, 3243
Bst4CI ACNGT 9 cut(s) 227, 1051, 1909, 2165, 2612, 2716, 2783, 3043, 3429
Bst6I CTCTTC 1 cut(s) 3276
BstACI GRCGYC 3 cut(s) 117, 213, 890
BstAFI CTTAAG 1 cut(s) 1127
BstBI TTCGAA 1 cut(s) 2955
BstDSI CCRYGG 1 cut(s) 2458
BstEII GGTNACC 3 cut(s) 476, 1614, 2783
BstF5I GGATG 4 cut(s) 854, 1063, 2625, 3269
BstFNI CGCG 5 cut(s) 83, 210, 379, 387, 578
BstH2I RGCGCY 1 cut(s) 737
BstMAI GTCTC 8 cut(s) 244, 941, 1331, 1656, 2149, 2284, 2516, 3067
BstMCI CGRYCG 1 cut(s) 277
BstNI CCWGG 7 cut(s) 146, 695, 1018, 1114, 2103, 2124, 3243
BstNSI RCATGY 3 cut(s) 960, 2665, 2776
BstPI GGTNACC 3 cut(s) 476, 1614, 2783
BstSFI CTRYAG 3 cut(s) 398, 455, 665
BstUI CGCG 5 cut(s) 83, 210, 379, 387, 578
BstV2I GAAGAC 4 cut(s) 385, 2420, 3180, 3186
BstX2I RGATCY 5 cut(s) 148, 982, 1918, 2068, 2263
BstYI RGATCY 5 cut(s) 148, 982, 1918, 2068, 2263
BstZ17I GTATAC 1 cut(s) 2027
BstZI CGGCCG 1 cut(s) 274
Bsu15I ATCGAT 1 cut(s) 1216
Bsu36I CCTNAGG 2 cut(s) 1380, 2750
BsuTUI ATCGAT 1 cut(s) 1216
BtgI CCRYGG 1 cut(s) 2458
BtgZI GCGATG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 2920
BtsCI GGATG 4 cut(s) 854, 1063, 2625, 3269
BtsI GCAGTG 1 cut(s) 2431
BtsIMutI CAGTG 7 cut(s) 289, 1056, 1432, 1914, 2063, 2181, 2431
BtuMI TCGCGA 1 cut(s) 578
CaiI CAGNNNCTG 1 cut(s) 817
Cfr10I RCCGGY 5 cut(s) 473, 2735, 3157, 3339, 3433
ClaI ATCGAT 1 cut(s) 1216
CpoI CGGWCCG 1 cut(s) 1154
CseI GACGC 6 cut(s) 125, 202, 712, 739, 879, 2188
Csp6I GTAC 4 cut(s) 526, 613, 1037, 2909
CspI CGGWCCG 1 cut(s) 1154
CviAII CATG 9 cut(s) 307, 957, 2494, 2662, 2773, 2828, 3349, 3367, 3438
CviQI GTAC 4 cut(s) 526, 613, 1037, 2909
DrdI GACNNNNNNGTC 2 cut(s) 709, 2828
DriI GACNNNNNGTC 2 cut(s) 2038, 3265
DseDI GACNNNNNNGTC 2 cut(s) 709, 2828
EaeI YGGCCR 3 cut(s) 274, 2824, 2840
EagI CGGCCG 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 3276
Eam1105I GACNNNNNGTC 2 cut(s) 2038, 3265
EarI CTCTTC 1 cut(s) 3276
EciI GGCGGA 1 cut(s) 3397
Ecl136II GAGCTC 4 cut(s) 247, 508, 797, 3139
EclXI CGGCCG 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 1207
Eco24I GRGCYC 4 cut(s) 249, 510, 799, 3141
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 8 cut(s) 682, 850, 1154, 1420, 1805, 2738, 3337, 3389
Eco52I CGGCCG 1 cut(s) 274
Eco53kI GAGCTC 4 cut(s) 247, 508, 797, 3139
Eco57I CTGAAG 5 cut(s) 115, 578, 794, 971, 1692
Eco81I CCTNAGG 2 cut(s) 1380, 2750
Eco88I CYCGRG 5 cut(s) 1294, 1817, 3134, 3251, 3263
Eco91I GGTNACC 3 cut(s) 476, 1614, 2783
EcoICRI GAGCTC 4 cut(s) 247, 508, 797, 3139
EcoO65I GGTNACC 3 cut(s) 476, 1614, 2783
EcoRI GAATTC 1 cut(s) 1864
EcoRII CCWGG 7 cut(s) 144, 693, 1016, 1112, 2101, 2122, 3241
EcoT14I CCWWGG 1 cut(s) 1207
EcoT22I ATGCAT 2 cut(s) 1726, 2206
EcoT38I GRGCYC 4 cut(s) 249, 510, 799, 3141
ErhI CCWWGG 1 cut(s) 1207
Esp3I CGTCTC 2 cut(s) 1656, 3067
FaeI CATG 9 cut(s) 310, 960, 2497, 2665, 2776, 2831, 3352, 3370, 3441
FaqI GGGAC 3 cut(s) 715, 836, 3061
FatI CATG 9 cut(s) 306, 956, 2493, 2661, 2772, 2827, 3348, 3366, 3437
FauI CCCGC 2 cut(s) 2084, 3339
FauNDI CATATG 1 cut(s) 2578
FblI GTMKAC 1 cut(s) 2026
FokI GGATG 4 cut(s) 841, 1070, 2612, 3256
FriOI GRGCYC 4 cut(s) 249, 510, 799, 3141
GsaI CCCAGC 2 cut(s) 175, 314
GsuI CTGGAG 5 cut(s) 128, 1096, 1355, 1680, 2427
HaeII RGCGCY 1 cut(s) 737
HgaI GACGC 6 cut(s) 125, 202, 712, 739, 879, 2188
Hin1I GRCGYC 3 cut(s) 117, 213, 890
Hin1II CATG 9 cut(s) 310, 960, 2497, 2665, 2776, 2831, 3352, 3370, 3441
HincII GTYRAC 1 cut(s) 2134
HindII GTYRAC 1 cut(s) 2134
HindIII AAGCTT 2 cut(s) 806, 2486
HpaI GTTAAC 1 cut(s) 2134
Hpy166II GTNNAC 8 cut(s) 526, 927, 2027, 2134, 2741, 2911, 3112, 3168
Hpy8I GTNNAC 8 cut(s) 526, 927, 2027, 2134, 2741, 2911, 3112, 3168
HpyCH4III ACNGT 9 cut(s) 227, 1051, 1909, 2165, 2612, 2716, 2783, 3043, 3429
HpyCH4IV ACGT 4 cut(s) 468, 1665, 2919, 3060
HpySE526I ACGT 4 cut(s) 468, 1665, 2919, 3060
Hsp92I GRCGYC 3 cut(s) 117, 213, 890
Hsp92II CATG 9 cut(s) 310, 960, 2497, 2665, 2776, 2831, 3352, 3370, 3441
Kpn2I TCCGGA 1 cut(s) 1741
KpnI GGTACC 1 cut(s) 1040
KroI GCCGGC 1 cut(s) 3433
KroNI GCCGGC 1 cut(s) 3435
KspAI GTTAAC 1 cut(s) 2134
LweI GCATC 6 cut(s) 582, 2123, 2213, 2632, 2819, 2935
MaeII ACGT 4 cut(s) 468, 1665, 2919, 3060
MbiI CCGCTC 2 cut(s) 194, 2091
MfeI CAATTG 3 cut(s) 724, 2526, 2836
MflI RGATCY 5 cut(s) 148, 982, 1918, 2068, 2263
MhlI GDGCHC 6 cut(s) 51, 249, 510, 799, 970, 3141
MlsI TGGCCA 1 cut(s) 2842
MluNI TGGCCA 1 cut(s) 2842
MmeI TCCRAC 7 cut(s) 289, 772, 1035, 1812, 1980, 3367, 3378
Mox20I TGGCCA 1 cut(s) 2842
Mph1103I ATGCAT 2 cut(s) 1726, 2206
MroI TCCGGA 1 cut(s) 1741
MroNI GCCGGC 1 cut(s) 3433
MroXI GAANNNNTTC 2 cut(s) 548, 836
MscI TGGCCA 1 cut(s) 2842
MslI CAYNNNNRTG 1 cut(s) 3274
Msp20I TGGCCA 1 cut(s) 2842
MspA1I CMGCKG 2 cut(s) 664, 3334
MspCI CTTAAG 1 cut(s) 1127
MteI GCGCNGCGC 1 cut(s) 86
MunI CAATTG 3 cut(s) 724, 2526, 2836
Mva1269I GAATGC 2 cut(s) 351, 766
MvaI CCWGG 7 cut(s) 146, 695, 1018, 1114, 2103, 2124, 3243
MvnI CGCG 5 cut(s) 83, 210, 379, 387, 578
NaeI GCCGGC 1 cut(s) 3435
NciI CCSGG 5 cut(s) 418, 431, 451, 1095, 3383
NdeI CATATG 1 cut(s) 2578
NgoMIV GCCGGC 1 cut(s) 3433
NlaIII CATG 9 cut(s) 310, 960, 2497, 2665, 2776, 2831, 3352, 3370, 3441
NmeAIII GCCGAG 3 cut(s) 133, 249, 747
NmuCI GTSAC 5 cut(s) 284, 1733, 2176, 3091, 3223
NruI TCGCGA 1 cut(s) 578
NsiI ATGCAT 2 cut(s) 1726, 2206
NspI RCATGY 3 cut(s) 960, 2665, 2776
NspV TTCGAA 1 cut(s) 2955
PaeR7I CTCGAG 2 cut(s) 3134, 3251
PciI ACATGT 2 cut(s) 956, 2661
PcsI WCGNNNNNNNCGW 4 cut(s) 528, 2913, 3066, 3132
PctI GAATGC 2 cut(s) 351, 766
PdiI GCCGGC 1 cut(s) 3435
PdmI GAANNNNTTC 2 cut(s) 548, 836
PfoI TCCNGGA 3 cut(s) 144, 416, 693
PscI ACATGT 2 cut(s) 956, 2661
Psp124BI GAGCTC 4 cut(s) 249, 510, 799, 3141
Psp6I CCWGG 7 cut(s) 144, 693, 1016, 1112, 2101, 2122, 3241
PspEI GGTNACC 3 cut(s) 476, 1614, 2783
PspFI CCCAGC 2 cut(s) 171, 310
PspGI CCWGG 7 cut(s) 144, 693, 1016, 1112, 2101, 2122, 3241
PspXI VCTCGAGB 2 cut(s) 3134, 3251
PstI CTGCAG 3 cut(s) 402, 459, 669
PstNI CAGNNNCTG 1 cut(s) 817
PsuI RGATCY 5 cut(s) 148, 982, 1918, 2068, 2263
PvuII CAGCTG 1 cut(s) 664
RruI TCGCGA 1 cut(s) 578
RsaI GTAC 4 cut(s) 527, 614, 1038, 2910
RsaNI GTAC 4 cut(s) 526, 613, 1037, 2909
RseI CAYNNNNRTG 1 cut(s) 3274
Rsr2I CGGWCCG 1 cut(s) 1154
RsrII CGGWCCG 1 cut(s) 1154
SacI GAGCTC 4 cut(s) 249, 510, 799, 3141
SduI GDGCHC 6 cut(s) 51, 249, 510, 799, 970, 3141
SfaNI GCATC 6 cut(s) 582, 2123, 2213, 2632, 2819, 2935
SfcI CTRYAG 3 cut(s) 398, 455, 665
Sfr274I CTCGAG 2 cut(s) 3134, 3251
SfuI TTCGAA 1 cut(s) 2955
SinI GGWCC 8 cut(s) 682, 850, 1154, 1420, 1805, 2738, 3337, 3389
SlaI CTCGAG 2 cut(s) 3134, 3251
SmiMI CAYNNNNRTG 1 cut(s) 3274
SmlI CTYRAG 4 cut(s) 1127, 2371, 3134, 3251
SmoI CTYRAG 4 cut(s) 1127, 2371, 3134, 3251
SpeI ACTAGT 1 cut(s) 2447
SstI GAGCTC 4 cut(s) 249, 510, 799, 3141
StyI CCWWGG 1 cut(s) 1207
TaaI ACNGT 9 cut(s) 227, 1051, 1909, 2165, 2612, 2716, 2783, 3043, 3429
TaiI ACGT 4 cut(s) 471, 1668, 2922, 3063
TaqII GACCGA 1 cut(s) 1752
TscAI CASTG 7 cut(s) 289, 1056, 1432, 1914, 2070, 2181, 2431
TseFI GTSAC 5 cut(s) 284, 1733, 2176, 3091, 3223
Tsp45I GTSAC 5 cut(s) 284, 1733, 2176, 3091, 3223
TspDTI ATGAA 7 cut(s) 17, 1134, 1770, 1877, 2165, 2670, 3355
TspGWI ACGGA 3 cut(s) 944, 2618, 3242
TspRI CASTG 7 cut(s) 289, 1056, 1432, 1914, 2070, 2181, 2431
Vha464I CTTAAG 1 cut(s) 1127
VpaK11BI GGWCC 8 cut(s) 682, 850, 1154, 1420, 1805, 2738, 3337, 3389
XapI RAATTY 4 cut(s) 1230, 1864, 2097, 2707
XbaI TCTAGA 2 cut(s) 1849, 2266
XceI RCATGY 3 cut(s) 960, 2665, 2776
XcmI CCANNNNNNNNNTGG 1 cut(s) 152
XhoI CTCGAG 2 cut(s) 3134, 3251
XmiI GTMKAC 1 cut(s) 2026
XmnI GAANNNNTTC 2 cut(s) 548, 836
Zsp2I ATGCAT 2 cut(s) 1726, 2206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.