AT5G01710

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013085
Physical Location & Seq
Reverse (-)
263511 .. 265633
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01710.1

Sequence Viewer

Length: 1542 bp
ATGGAGACTTCCATGGGGAAACCCAGCTTCTTACGGAATATAATGGTTCGCGTGCTTCTCTTTGGTGTTCTTATCATCGTTGTGAGATTTGCTTATGTTGTCACCATCACCGGTGAATCATGCAATCGCGGCGACTTCTGCTTCTTCTCTCTCCCTGAGAATCTCAACTTTGTCATCTCCGGTGCTGCCTCCGGCTCTTCTGCTATCGACGCCATCAGATCTACTTCCCCTGGGGATGATCTCTACACCACCAGGGATTGGATCAAATCCGTTCAGTTTTACTCCTCCATCTTCCAGGATCTGATCGCCGACGGTTATCTATCGCCTGAATCGAAGACGCTTTGTGTAGAGACGGCGATTGGACAAGAGGTTCACTCGCTCAGAGAGATTGGGGTGAAGAACTCCGTCGGGATCTCTAAGAAAGCTTCTAGGCCGTTGGTGGTTAGAGGCGAGGGACATGCTATTCCCTTTGAGGATAACGCTTTCGATTTTGTATTCTCCGGTGGTGACCGCCTTGGGAAGTCGTTGAAGCAGTTGGAATTCGCCGATGAGATTACTCGAACACTGAAACCCGAAGGGTTTGCGGTGGTTCATGTTGGGGCTACAGATACGTACAGTTTCAATTCATTCCTTGATTTGTTTAATTCTTGCAGATTGGTGAAGATGCGTGATATCGATGGTTTTGATCCTTCGATGCCTCATATCAGAGAATTTGTTATTCAGAAATACTCAGAGATTGATGGTGGTGGTCATCACCACCGTGGTGGTGGTGATTCTGGTGGAAAGTGCTGGATTCCGGGTTACAAAAGAGATTTGATTCGGGATGCAGAGCCACTGATTCAAGAGGAGCCTCTTAAGCCATGGATTACACTTAAGAGGAACATCAAGAATATTAAATATGTGCCTTCTATGGTTGATATACGTTTCAAGAGTAGATACGTTTACGTTGATGTTGGTGCTAGAAGTTATGGATCCAGTATTGGGAGTTGGTTCAAGAAAGAGTACCCTAAGCAGAACAAAACTTTTGATGTTTTCGCCATTGAGGCTGACAAAGCCTTTCACGAAGAGTATAAGATAAAGAAGAAGGTGAACCTGTTGCCATACGCAGCGTGGGTGAGGAACGAGACGTTATCGTTTGAGATCAATCACGATCCGGGAAAGGAAGTGGAAGCCAAAGCAAAGGGCAGGGGGATGGGCAGAATCCAGCCGGTTAAAAAGTCTGACTCGAGTGACTTAGCTGGAGAGGTGAATTTGATTCAAGGGTTTGATTTCGCTGACTGGTTGAAGAAGAGTGTGAGAGAGAGGGACTTTGTTGTGATGAAGATGGATGTTGAAGGAACCGAATTCGATCTGATCCCGAGGCTAATCAAGACAGGAGCGATTTGTCTAATAGATGAGTTGTTCCTTGAATGCCATTACAACAGGTGGCAGAGATGTTGTCCTGGTCAAAGGAGCCAAAAGTACAACAAGACTTATAACCAATGCTTAGAGCTCTTCAATTCGCTCAGACAAAGAGGGGTGCTTGTCCATCAATGGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

513

Amino Acids

58.29

Weight (kDa)

8.5

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 277 - 374 5.2e-10 Domain of unknown function (DUF7870)
Methyltransf_21 PF05050 317 - 493 3.5e-06 Methyltransferase FkbM domain
DUF7870 PF25276 419 - 513 1.3e-23 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01710
fragaria_vesca FvH4_6g10540
malus_domestica MD04G1167500.v1.1 MD12G1180800.v1.1
prunus_persica Prupe.6G285700_v2.0.a1
pyrus_communis pycom04g14860 pycom12g17040
rosa_chinensis RchiOBHm_Chr3g0461501
rosa_laevigata RLG00000024927
rosa_multiflora Rmu_sc0002677.1_g000002
rosa_roxburghii Rroxscaffold_6G00418230 Rroxscaffold_6G00418560
rosa_rugosa Rorug03G0051300
rosa_samantha Rh3AG109100 Rh3BG112900 Rh3CG114400 Rh3DG114500
rosa_wichuraiana Rw3G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1476
AccB7I CCANNNNNTGG 1 cut(s) 258
AccII CGCG 2 cut(s) 51, 129
AciI CCGC 3 cut(s) 129, 511, 584
AclWI GGATC 8 cut(s) 269, 306, 419, 680, 966, 979, 1145, 1348
AcsI RAATTY 4 cut(s) 539, 710, 1249, 1343
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 3 cut(s) 614, 1004, 1463
AfiI CCNNNNNNNGG 2 cut(s) 258, 981
AflII CTTAAG 2 cut(s) 854, 872
AgeI ACCGGT 1 cut(s) 110
AjnI CCWGG 4 cut(s) 229, 251, 294, 1441
AleI CACNNNNGTG 2 cut(s) 759, 762
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
AluBI AGCT 4 cut(s) 27, 425, 1238, 1492
AluI AGCT 4 cut(s) 27, 425, 1238, 1492
Alw21I GWGCWC 1 cut(s) 1494
Alw26I GTCTC 2 cut(s) 344, 1118
AlwI GGATC 8 cut(s) 269, 306, 419, 680, 966, 979, 1145, 1348
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 2 cut(s) 1225, 1357
AoxI GGCC 1 cut(s) 431
ApeKI GCWGC 2 cut(s) 185, 1106
ApoI RAATTY 4 cut(s) 539, 710, 1249, 1343
AsiGI ACCGGT 1 cut(s) 110
AsuC2I CCSGG 2 cut(s) 798, 1155
AvaI CYCGRG 2 cut(s) 1225, 1357
BamHI GGATCC 1 cut(s) 971
BanII GRGCYC 1 cut(s) 1494
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 1494
BbvI GCAGC 2 cut(s) 172, 1118
BccI CCATC 8 cut(s) 113, 221, 296, 671, 734, 1186, 1318, 1536
BceAI ACGGC 2 cut(s) 369, 418
BcgI CGANNNNNNTGC 4 cut(s) 440, 474, 563, 597
BciT130I CCWGG 4 cut(s) 231, 253, 296, 1443
BcnI CCSGG 2 cut(s) 798, 1155
BcoDI GTCTC 2 cut(s) 344, 1118
BfaI CTAG 2 cut(s) 429, 960
BfmI CTRYAG 1 cut(s) 603
BfrI CTTAAG 2 cut(s) 854, 872
BglI GCCNNNNNGGC 1 cut(s) 1043
BglII AGATCT 1 cut(s) 218
BisI GCNGC 3 cut(s) 130, 186, 1107
BlsI GCNGC 3 cut(s) 131, 187, 1108
Bme1390I CCNGG 6 cut(s) 231, 253, 296, 798, 1155, 1443
BmeT110I CYCGRG 2 cut(s) 1225, 1357
BmiI GGNNCC 4 cut(s) 849, 973, 1339, 1454
BmrFI CCNGG 6 cut(s) 231, 253, 296, 798, 1155, 1443
BmsI GCATC 3 cut(s) 654, 684, 814
BpiI GAAGAC 1 cut(s) 341
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 1 cut(s) 1260
Bpu10I CCTNAGC 1 cut(s) 1008
BpuMI CCSGG 2 cut(s) 798, 1155
Bsa29I ATCGAT 1 cut(s) 675
BsaAI YACGTR 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 8 cut(s) 12, 229, 230, 252, 514, 760, 860, 1358
BsaWI WCCGGW 3 cut(s) 110, 179, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 981
Bse118I RCCGGY 2 cut(s) 110, 1207
Bse1I ACTGG 2 cut(s) 975, 1283
BseBI CCWGG 4 cut(s) 231, 253, 296, 1443
BseCI ATCGAT 1 cut(s) 675
BseDI CCNNGG 8 cut(s) 12, 229, 230, 252, 514, 760, 860, 1358
BseGI GGATG 4 cut(s) 241, 829, 1197, 1333
BseLI CCNNNNNNNGG 2 cut(s) 258, 981
BseMII CTCAG 4 cut(s) 147, 394, 744, 1519
BseNI ACTGG 2 cut(s) 975, 1283
BseRI GAGGAG 2 cut(s) 274, 860
BseXI GCAGC 2 cut(s) 172, 1118
BseYI CCCAGC 1 cut(s) 23
Bsh1236I CGCG 2 cut(s) 51, 129
BshFI GGCC 1 cut(s) 433
BshTI ACCGGT 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 675
BsiHKAI GWGCWC 1 cut(s) 1494
BsiHKCI CYCGRG 2 cut(s) 1225, 1357
BsiSI CCGG 7 cut(s) 111, 180, 192, 501, 797, 1154, 1208
BslFI GGGAC 2 cut(s) 468, 1319
BslI CCNNNNNNNGG 2 cut(s) 258, 981
BsmAI GTCTC 2 cut(s) 344, 1118
BsmBI CGTCTC 2 cut(s) 344, 1118
BsmFI GGGAC 2 cut(s) 468, 1319
BsmI GAATGC 1 cut(s) 1415
BsnI GGCC 1 cut(s) 433
BsoBI CYCGRG 2 cut(s) 1225, 1357
Bsp1286I GDGCHC 1 cut(s) 1494
Bsp19I CCATGG 2 cut(s) 12, 860
BspACI CCGC 3 cut(s) 129, 511, 584
BspANI GGCC 1 cut(s) 433
BspCNI CTCAG 4 cut(s) 148, 393, 743, 1518
BspDI ATCGAT 1 cut(s) 675
BspFNI CGCG 2 cut(s) 51, 129
BspLI GGNNCC 4 cut(s) 849, 973, 1339, 1454
BspPI GGATC 8 cut(s) 269, 306, 419, 680, 966, 979, 1145, 1348
BspQI GCTCTTC 2 cut(s) 202, 1499
BspTI CTTAAG 2 cut(s) 854, 872
BsrFI RCCGGY 2 cut(s) 110, 1207
BsrI ACTGG 2 cut(s) 975, 1283
BssAI RCCGGY 2 cut(s) 110, 1207
BssECI CCNNGG 8 cut(s) 12, 229, 230, 252, 514, 760, 860, 1358
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 3 cut(s) 12, 514, 860
Bst2UI CCWGG 4 cut(s) 231, 253, 296, 1443
Bst4CI ACNGT 3 cut(s) 314, 617, 761
Bst6I CTCTTC 4 cut(s) 202, 1059, 1283, 1499
BstACI GRCGYC 1 cut(s) 210
BstAFI CTTAAG 2 cut(s) 854, 872
BstBAI YACGTR 1 cut(s) 612
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 8 cut(s) 156, 380, 417, 730, 1008, 1234, 1486, 1505
BstDSI CCRYGG 3 cut(s) 12, 760, 860
BstEII GGTNACC 1 cut(s) 506
BstF5I GGATG 4 cut(s) 241, 829, 1197, 1333
BstFNI CGCG 2 cut(s) 51, 129
BstMAI GTCTC 2 cut(s) 344, 1118
BstMWI GCNNNNNNNGC 6 cut(s) 129, 138, 209, 856, 1043, 1052
BstNI CCWGG 4 cut(s) 231, 253, 296, 1443
BstNSI RCATGY 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 506
BstSCI CCNGG 6 cut(s) 229, 251, 294, 796, 1153, 1441
BstSFI CTRYAG 1 cut(s) 603
BstSNI TACGTA 1 cut(s) 612
BstUI CGCG 2 cut(s) 51, 129
BstV1I GCAGC 2 cut(s) 172, 1118
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 4 cut(s) 218, 298, 411, 971
BstXI CCANNNNNNTGG 1 cut(s) 764
BstYI RGATCY 4 cut(s) 218, 298, 411, 971
Bsu15I ATCGAT 1 cut(s) 675
BsuRI GGCC 1 cut(s) 433
BsuTUI ATCGAT 1 cut(s) 675
BtgI CCRYGG 3 cut(s) 12, 760, 860
BtsCI GGATG 4 cut(s) 241, 829, 1197, 1333
BtsIMutI CAGTG 2 cut(s) 563, 833
Cac8I GCNNGC 1 cut(s) 53
CaiI CAGNNNCTG 1 cut(s) 301
Cfr10I RCCGGY 2 cut(s) 110, 1207
ClaI ATCGAT 1 cut(s) 675
CseI GACGC 2 cut(s) 218, 346
Csp6I GTAC 3 cut(s) 613, 1003, 1462
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 5 cut(s) 13, 120, 458, 593, 861
CviQI GTAC 3 cut(s) 613, 1003, 1462
DdeI CTNAG 8 cut(s) 156, 380, 417, 730, 1008, 1234, 1486, 1505
Eam1104I CTCTTC 4 cut(s) 202, 1059, 1283, 1499
EarI CTCTTC 4 cut(s) 202, 1059, 1283, 1499
Ecl136II GAGCTC 1 cut(s) 1492
Eco105I TACGTA 1 cut(s) 612
Eco130I CCWWGG 3 cut(s) 12, 514, 860
Eco24I GRGCYC 1 cut(s) 1494
Eco32I GATATC 1 cut(s) 673
Eco53kI GAGCTC 1 cut(s) 1492
Eco88I CYCGRG 2 cut(s) 1225, 1357
Eco91I GGTNACC 1 cut(s) 506
EcoICRI GAGCTC 1 cut(s) 1492
EcoO65I GGTNACC 1 cut(s) 506
EcoRI GAATTC 2 cut(s) 539, 1343
EcoRII CCWGG 4 cut(s) 229, 251, 294, 1441
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 3 cut(s) 12, 514, 860
EcoT38I GRGCYC 1 cut(s) 1494
ErhI CCWWGG 3 cut(s) 12, 514, 860
Esp3I CGTCTC 2 cut(s) 344, 1118
FaeI CATG 5 cut(s) 16, 123, 461, 596, 864
FaqI GGGAC 2 cut(s) 468, 1319
FatI CATG 5 cut(s) 12, 119, 457, 592, 860
Fnu4HI GCNGC 3 cut(s) 130, 186, 1107
FokI GGATG 4 cut(s) 248, 836, 1204, 1340
FriOI GRGCYC 1 cut(s) 1494
Fsp4HI GCNGC 3 cut(s) 130, 186, 1107
FspBI CTAG 2 cut(s) 429, 960
GluI GCNGC 3 cut(s) 130, 186, 1107
GsaI CCCAGC 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 1260
HaeIII GGCC 1 cut(s) 433
HapII CCGG 7 cut(s) 111, 180, 192, 501, 797, 1154, 1208
HgaI GACGC 2 cut(s) 218, 346
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 5 cut(s) 16, 123, 461, 596, 864
HindIII AAGCTT 1 cut(s) 423
HpaII CCGG 7 cut(s) 111, 180, 192, 501, 797, 1154, 1208
Hpy166II GTNNAC 3 cut(s) 373, 943, 1090
Hpy188I TCNGA 9 cut(s) 218, 303, 383, 707, 723, 733, 1222, 1353, 1508
Hpy8I GTNNAC 3 cut(s) 373, 943, 1090
Hpy99I CGWCG 3 cut(s) 212, 314, 410
HpyAV CCTTC 5 cut(s) 569, 699, 915, 1078, 1328
HpyCH4III ACNGT 3 cut(s) 314, 617, 761
HpyCH4IV ACGT 5 cut(s) 611, 922, 939, 945, 1127
HpyCH4V TGCA 3 cut(s) 123, 651, 827
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 138, 209, 856, 1043, 1052
HpyF3I CTNAG 8 cut(s) 156, 380, 417, 730, 1008, 1234, 1486, 1505
HpySE526I ACGT 5 cut(s) 611, 922, 939, 945, 1127
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 5 cut(s) 16, 123, 461, 596, 864
LguI GCTCTTC 2 cut(s) 202, 1499
LmnI GCTCC 3 cut(s) 847, 1376, 1452
Lsp1109I GCAGC 2 cut(s) 172, 1118
LweI GCATC 3 cut(s) 654, 684, 814
MaeI CTAG 2 cut(s) 429, 960
MaeII ACGT 5 cut(s) 611, 922, 939, 945, 1127
MaeIII GTNAC 4 cut(s) 100, 506, 800, 1229
MflI RGATCY 4 cut(s) 218, 298, 411, 971
MhlI GDGCHC 1 cut(s) 1494
MluCI AATT 7 cut(s) 539, 622, 643, 710, 1249, 1343, 1498
MlyI GAGTC 1 cut(s) 1217
MmeI TCCRAC 1 cut(s) 516
MseI TTAA 5 cut(s) 642, 855, 873, 894, 1212
MslI CAYNNNNRTG 3 cut(s) 80, 759, 762
MspCI CTTAAG 2 cut(s) 854, 872
MspI CCGG 7 cut(s) 111, 180, 192, 501, 797, 1154, 1208
MspR9I CCNGG 6 cut(s) 231, 253, 296, 798, 1155, 1443
Mva1269I GAATGC 1 cut(s) 1415
MvaI CCWGG 4 cut(s) 231, 253, 296, 1443
MvnI CGCG 2 cut(s) 51, 129
MwoI GCNNNNNNNGC 6 cut(s) 129, 138, 209, 856, 1043, 1052
NciI CCSGG 2 cut(s) 798, 1155
NcoI CCATGG 2 cut(s) 12, 860
NlaIII CATG 5 cut(s) 16, 123, 461, 596, 864
NlaIV GGNNCC 4 cut(s) 849, 973, 1339, 1454
NmuCI GTSAC 3 cut(s) 100, 506, 1229
NspI RCATGY 1 cut(s) 461
OliI CACNNNNGTG 2 cut(s) 759, 762
PaeR7I CTCGAG 1 cut(s) 1225
PasI CCCWGGG 1 cut(s) 230
PciSI GCTCTTC 2 cut(s) 202, 1499
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PctI GAATGC 1 cut(s) 1415
PfeI GAWTC 9 cut(s) 116, 160, 329, 773, 793, 817, 838, 1200, 1255
PflMI CCANNNNNTGG 1 cut(s) 258
PfoI TCCNGGA 2 cut(s) 294, 1153
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 3 cut(s) 131, 187, 1108
PleI GAGTC 1 cut(s) 1217
PpsI GAGTC 1 cut(s) 1217
Ppu21I YACGTR 1 cut(s) 612
PsiI TTATAA 1 cut(s) 1476
Psp124BI GAGCTC 1 cut(s) 1494
Psp6I CCWGG 4 cut(s) 229, 251, 294, 1441
PspEI GGTNACC 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 4 cut(s) 229, 251, 294, 1441
PspN4I GGNNCC 4 cut(s) 849, 973, 1339, 1454
PspXI VCTCGAGB 1 cut(s) 1225
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 4 cut(s) 218, 298, 411, 971
RsaI GTAC 3 cut(s) 614, 1004, 1463
RsaNI GTAC 3 cut(s) 613, 1003, 1462
RseI CAYNNNNRTG 3 cut(s) 80, 759, 762
SacI GAGCTC 1 cut(s) 1494
SapI GCTCTTC 2 cut(s) 202, 1499
SaqAI TTAA 5 cut(s) 642, 855, 873, 894, 1212
SatI GCNGC 3 cut(s) 130, 186, 1107
SchI GAGTC 1 cut(s) 1217
ScrFI CCNGG 6 cut(s) 231, 253, 296, 798, 1155, 1443
SduI GDGCHC 1 cut(s) 1494
SfaNI GCATC 3 cut(s) 654, 684, 814
SfcI CTRYAG 1 cut(s) 603
Sfr274I CTCGAG 1 cut(s) 1225
SgrAI CRCCGGYG 1 cut(s) 110
SlaI CTCGAG 1 cut(s) 1225
SmiMI CAYNNNNRTG 3 cut(s) 80, 759, 762
SmlI CTYRAG 3 cut(s) 854, 872, 1225
SmoI CTYRAG 3 cut(s) 854, 872, 1225
SnaBI TACGTA 1 cut(s) 612
Sse9I AATT 7 cut(s) 539, 622, 643, 710, 1249, 1343, 1498
SsiI CCGC 3 cut(s) 129, 511, 584
SspI AATATT 1 cut(s) 892
SspMI CTAG 2 cut(s) 429, 960
SstI GAGCTC 1 cut(s) 1494
StyD4I CCNGG 6 cut(s) 229, 251, 294, 796, 1153, 1441
StyI CCWWGG 3 cut(s) 12, 514, 860
TaaI ACNGT 3 cut(s) 314, 617, 761
TaiI ACGT 5 cut(s) 614, 925, 942, 948, 1130
TaqI TCGA 8 cut(s) 207, 332, 486, 559, 675, 692, 1226, 1347
TasI AATT 7 cut(s) 539, 622, 643, 710, 1249, 1343, 1498
TatI WGTACW 1 cut(s) 1461
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 9 cut(s) 116, 160, 329, 773, 793, 817, 838, 1200, 1255
Tru1I TTAA 5 cut(s) 642, 855, 873, 894, 1212
Tru9I TTAA 5 cut(s) 642, 855, 873, 894, 1212
TscAI CASTG 2 cut(s) 570, 840
TseFI GTSAC 3 cut(s) 100, 506, 1229
TseI GCWGC 2 cut(s) 185, 1106
Tsp45I GTSAC 3 cut(s) 100, 506, 1229
TspDTI ATGAA 3 cut(s) 581, 615, 1334
TspGWI ACGGA 3 cut(s) 49, 259, 394
TspRI CASTG 2 cut(s) 570, 840
Van91I CCANNNNNTGG 1 cut(s) 258
Vha464I CTTAAG 2 cut(s) 854, 872
XapI RAATTY 4 cut(s) 539, 710, 1249, 1343
XceI RCATGY 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 2 cut(s) 764, 1107
XhoI CTCGAG 1 cut(s) 1225
XspI CTAG 2 cut(s) 429, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.