Rw3G009270

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013085
Physical Location & Seq
Forward (+)
8863993 .. 8865523
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009270.1

Sequence Viewer

Length: 1485 bp
ATGGAGCCTAATACAGTCAAGCCGAGGTTCCTGAAGAACACTCTGGTGCGGTGTTTCCTGGTCGGCGTCGTCATGGTACTGTTCCGGTTCGCTTACGTCATCACCGTCACCGGCGAGTCGTGTAAAGTCGGCAACTTTTGCTTTCTTAATCTTCCGGAGAATCTCAACTTCGTCATCGCCAGCACCGGAGCTTCCGCCGCCAGGAACGACATGTACACCAGCAAGGACTGGATCAAGGCCGTCCATTTTTACTCCTCCATTTTCCAAGATCTGATCTCGCAAGGGTTTCTGTCGCCGAAATCCAAGTCTCTGTGCGTCGAAACCCCGACGGGGCAGGAAGTCTACGCCTTGAAAGAGAGCGGCGTCAAGGACGCGGTCGGGATTTTCAAGAAAGCCTCGAGGCCTTTGGTGATTCGCGGCGAGGCGCACCGGATGCCGTTTGCCGATAACTCGTTCGACTTCGTTTTCTCCGGCAACGGCCGCCTCGACAAGTCGCCGAAGGCGGCGGATTTCGCGGCGGAGATTGTACGGACGCTCAGGCTCGAAGGGTTTGCGGTGGTCCACATCGGAGCCAAGGATACTTATAGCTTTCATTCGTTTATTGATTTGTTCAATTGCTGCAAGTTAGTTACTTCACGCGACATCGACGGCTTTAATTCGTCGATGCCGCACATACGTGAAATGGTGTTGAGGAAGGAGTGTGATGCTACTAATGAGGTTGTGAATTCCGGTAAGAAGTGCTCGGTTCCGGCGCATAAGCTTGAGTTGGTGAGGAAAGCGGAGCCAATTATTCAGATTGAGCCAATGAAGCCGTGGATTACTTTGAAGAAGAATATACAGAATGTGAAGTACCTTCCGTCGATGGCGGATATTAGCTTTAAGAGGAGGTATGCTTATGTTGATGTTGGAGCTAGGAGCTATGGTTCGAGTATTGGTAGCTGGTTCAAGAAGCAGTACCCGAAGCAGAATAAGACATTTGAAGTTTTCGCTATTGAGGCTGATAAGATTTTTCATGAACAGTACAAGCTGAAGAAGGGGATTACTTTGCTGCCGTATGCTGCTTGGGTGAGGAATGAGACATTGTCGTTTGAGATTAATGGAGACCCTGGCGAGAAGGTGAAGGAGAAAGGTAGAGGAATGGGGAGGATTCAGCCGGTGAAGTCGGTTAATGGGGGGTTTGATGGTGAGGTGGATAGGATTCAGGGGTTTGATTTTGCGGATTGGGTGAAGAATACTTTCACAGAGAAGGATTTCGTCGTGATGAAGATGGATGTGGAAGGGACGGAATTTGATTTGATTCCGAGGCTGTTTGAGACCGGAGCAATTTGTTTGATCGATGAGGTGTTTCTTGAATGCCATTACAATAGGTGGCAGAGGACACGCCCTGGTGAGAGAAGTGCAAAGTATGAAAAAACTTATGGCCAGTGCTTGGACTTGTTTACTTCACTAAGGCAAAGTGGGGTTCTTGTTCATCAGTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

494

Amino Acids

56.19

Weight (kDa)

9.02

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 135 - 186 9.9e-06 Methyltransferase domain
DUF7870 PF25276 267 - 338 3.9e-09 Domain of unknown function (DUF7870)
DUF7870 PF25276 400 - 494 4.5e-20 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01710
fragaria_vesca FvH4_6g10540
malus_domestica MD04G1167500.v1.1 MD12G1180800.v1.1
prunus_persica Prupe.6G285700_v2.0.a1
pyrus_communis pycom04g14860 pycom12g17040
rosa_chinensis RchiOBHm_Chr3g0461501
rosa_laevigata RLG00000024927
rosa_multiflora Rmu_sc0002677.1_g000002
rosa_roxburghii Rroxscaffold_6G00418230 Rroxscaffold_6G00418560
rosa_rugosa Rorug03G0051300
rosa_samantha Rh3AG109100 Rh3BG112900 Rh3CG114400 Rh3DG114500
rosa_wichuraiana Rw3G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 397
AccB7I CCANNNNNTGG 1 cut(s) 1429
AccBSI CCGCTC 1 cut(s) 360
AccI GTMKAC 1 cut(s) 342
AccII CGCG 4 cut(s) 374, 417, 515, 639
AccIII TCCGGA 1 cut(s) 154
AclWI GGATC 1 cut(s) 239
AcoI YGGCCR 2 cut(s) 478, 1420
AcsI RAATTY 2 cut(s) 724, 1286
AcuI CTGAAG 2 cut(s) 53, 1049
AcyI GRCGYC 2 cut(s) 66, 363
AfaI GTAC 6 cut(s) 78, 215, 528, 851, 956, 1022
AfiI CCNNNNNNNGG 3 cut(s) 201, 330, 1429
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 7 cut(s) 352, 388, 613, 826, 946, 980, 1352
AjnI CCWGG 4 cut(s) 57, 200, 1105, 1384
AleI CACNNNNGTG 2 cut(s) 44, 675
AloI GAACNNNNNNTCC 2 cut(s) 907, 939
AluBI AGCT 8 cut(s) 191, 588, 760, 876, 911, 918, 939, 1027
AluI AGCT 8 cut(s) 191, 588, 760, 876, 911, 918, 939, 1027
Alw21I GWGCWC 1 cut(s) 743
Alw26I GTCTC 4 cut(s) 312, 1070, 1095, 1307
AlwI GGATC 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 397
Aor13HI TCCGGA 1 cut(s) 154
AoxI GGCC 4 cut(s) 237, 401, 478, 1420
ApeKI GCWGC 3 cut(s) 618, 1048, 1058
ApoI RAATTY 2 cut(s) 724, 1286
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AseI ATTAAT 1 cut(s) 1095
Asp700I GAANNNNTTC 2 cut(s) 1235, 1250
AspLEI GCGC 2 cut(s) 427, 754
AspS9I GGNCC 1 cut(s) 559
AvaI CYCGRG 1 cut(s) 397
AvaII GGWCC 1 cut(s) 559
BalI TGGCCA 1 cut(s) 1422
Bbv12I GWGCWC 1 cut(s) 743
BbvI GCAGC 3 cut(s) 605, 1035, 1045
BccI CCATC 3 cut(s) 856, 1175, 1261
BceAI ACGGC 6 cut(s) 224, 421, 493, 664, 796, 1036
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 4 cut(s) 59, 202, 1107, 1386
BciVI GTATCC 1 cut(s) 571
BcoDI GTCTC 4 cut(s) 312, 1070, 1095, 1307
BfaI CTAG 1 cut(s) 912
BfuI GTATCC 1 cut(s) 571
BglII AGATCT 1 cut(s) 268
Bme1390I CCNGG 4 cut(s) 59, 202, 1107, 1386
Bme18I GGWCC 1 cut(s) 559
BmeT110I CYCGRG 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 559
BmiI GGNNCC 5 cut(s) 6, 29, 571, 747, 783
BmrFI CCNGG 4 cut(s) 59, 202, 1107, 1386
BmsI GCATC 3 cut(s) 423, 654, 694
Bpu10I CCTNAGC 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 782
Bsa29I ATCGAT 1 cut(s) 1335
BsaAI YACGTR 1 cut(s) 677
BsaHI GRCGYC 2 cut(s) 66, 363
BsaI GGTCTC 2 cut(s) 1095, 1307
BsaJI CCNNGG 6 cut(s) 23, 573, 812, 1105, 1301, 1384
BsaWI WCCGGW 6 cut(s) 84, 154, 185, 429, 728, 1316
BsaXI ACNNNNNCTCC 2 cut(s) 907, 937
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 330, 1429
Bse118I RCCGGY 2 cut(s) 110, 1153
Bse1I ACTGG 2 cut(s) 233, 1423
BseAI TCCGGA 1 cut(s) 154
BseBI CCWGG 4 cut(s) 59, 202, 1107, 1386
BseCI ATCGAT 1 cut(s) 1335
BseDI CCNNGG 6 cut(s) 23, 573, 812, 1105, 1301, 1384
BseGI GGATG 2 cut(s) 438, 1276
BseLI CCNNNNNNNGG 3 cut(s) 201, 330, 1429
BseMII CTCAG 1 cut(s) 550
BseNI ACTGG 2 cut(s) 233, 1423
BseRI GAGGAG 2 cut(s) 244, 898
BseX3I CGGCCG 1 cut(s) 478
BseXI GCAGC 3 cut(s) 605, 1035, 1045
Bsh1236I CGCG 4 cut(s) 374, 417, 515, 639
Bsh1285I CGRYCG 2 cut(s) 378, 481
BshFI GGCC 4 cut(s) 239, 403, 480, 1422
BshVI ATCGAT 1 cut(s) 1335
BsiEI CGRYCG 2 cut(s) 378, 481
BsiHKAI GWGCWC 1 cut(s) 743
BsiHKCI CYCGRG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 1294
BslI CCNNNNNNNGG 3 cut(s) 201, 330, 1429
BsmAI GTCTC 4 cut(s) 312, 1070, 1095, 1307
BsmFI GGGAC 1 cut(s) 1294
BsmI GAATGC 1 cut(s) 1358
BsnI GGCC 4 cut(s) 239, 403, 480, 1422
Bso31I GGTCTC 2 cut(s) 1095, 1307
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 743
Bsp13I TCCGGA 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 213
Bsp143I GATC 4 cut(s) 231, 268, 273, 1332
BspANI GGCC 4 cut(s) 239, 403, 480, 1422
BspCNI CTCAG 1 cut(s) 549
BspDI ATCGAT 1 cut(s) 1335
BspEI TCCGGA 1 cut(s) 154
BspFNI CGCG 4 cut(s) 374, 417, 515, 639
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 5 cut(s) 6, 29, 571, 747, 783
BspPI GGATC 1 cut(s) 239
BspTNI GGTCTC 2 cut(s) 1095, 1307
BsrBI CCGCTC 1 cut(s) 360
BsrFI RCCGGY 2 cut(s) 110, 1153
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 2 cut(s) 233, 1423
BssAI RCCGGY 2 cut(s) 110, 1153
BssECI CCNNGG 6 cut(s) 23, 573, 812, 1105, 1301, 1384
BssMI GATC 4 cut(s) 231, 268, 273, 1332
BssNI GRCGYC 2 cut(s) 66, 363
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 4 cut(s) 59, 202, 1107, 1386
Bst4CI ACNGT 4 cut(s) 16, 81, 106, 1020
BstACI GRCGYC 2 cut(s) 66, 363
BstAPI GCANNNNNTGC 2 cut(s) 138, 433
BstAUI TGTACA 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 2 cut(s) 536, 1448
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 2 cut(s) 438, 1276
BstFNI CGCG 4 cut(s) 374, 417, 515, 639
BstHHI GCGC 2 cut(s) 427, 754
BstKTI GATC 4 cut(s) 234, 271, 276, 1335
BstMAI GTCTC 4 cut(s) 312, 1070, 1095, 1307
BstMBI GATC 4 cut(s) 231, 268, 273, 1332
BstMCI CGRYCG 2 cut(s) 378, 481
BstMWI GCNNNNNNNGC 7 cut(s) 138, 197, 433, 480, 512, 808, 995
BstNI CCWGG 4 cut(s) 59, 202, 1107, 1386
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 4 cut(s) 57, 200, 1105, 1384
BstUI CGCG 4 cut(s) 374, 417, 515, 639
BstV1I GCAGC 3 cut(s) 605, 1035, 1045
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BstZI CGGCCG 1 cut(s) 478
Bsu15I ATCGAT 1 cut(s) 1335
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 4 cut(s) 239, 403, 480, 1422
BsuTUI ATCGAT 1 cut(s) 1335
BtgI CCRYGG 1 cut(s) 812
BtgZI GCGATG 1 cut(s) 160
BtsCI GGATG 2 cut(s) 438, 1276
BtsIMutI CAGTG 2 cut(s) 1430, 1481
Cac8I GCNNGC 1 cut(s) 181
CciI TCATGA 1 cut(s) 1012
CfoI GCGC 2 cut(s) 427, 754
Cfr10I RCCGGY 2 cut(s) 110, 1153
Cfr13I GGNCC 1 cut(s) 559
ClaI ATCGAT 1 cut(s) 1335
CseI GACGC 5 cut(s) 55, 304, 352, 380, 541
Csp6I GTAC 6 cut(s) 77, 214, 527, 850, 955, 1021
CviAII CATG 3 cut(s) 73, 211, 1013
CviQI GTAC 6 cut(s) 77, 214, 527, 850, 955, 1021
DdeI CTNAG 2 cut(s) 536, 1448
DpnI GATC 4 cut(s) 233, 270, 275, 1334
DpnII GATC 4 cut(s) 231, 268, 273, 1332
EaeI YGGCCR 2 cut(s) 478, 1420
EagI CGGCCG 1 cut(s) 478
EciI GGCGGA 4 cut(s) 184, 521, 533, 881
EclXI CGGCCG 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 573
Eco147I AGGCCT 1 cut(s) 403
Eco31I GGTCTC 2 cut(s) 1095, 1307
Eco47I GGWCC 1 cut(s) 559
Eco52I CGGCCG 1 cut(s) 478
Eco57I CTGAAG 2 cut(s) 53, 1049
Eco88I CYCGRG 1 cut(s) 397
EcoRI GAATTC 1 cut(s) 724
EcoRII CCWGG 4 cut(s) 57, 200, 1105, 1384
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 3 cut(s) 76, 214, 1016
FaqI GGGAC 1 cut(s) 1294
FatI CATG 3 cut(s) 72, 210, 1012
FblI GTMKAC 1 cut(s) 342
FokI GGATG 2 cut(s) 445, 1283
FspBI CTAG 1 cut(s) 912
GlaI GCGC 2 cut(s) 426, 753
HaeIII GGCC 4 cut(s) 239, 403, 480, 1422
HgaI GACGC 5 cut(s) 55, 304, 352, 380, 541
HhaI GCGC 2 cut(s) 427, 754
Hin1I GRCGYC 2 cut(s) 66, 363
Hin1II CATG 3 cut(s) 76, 214, 1016
Hin6I GCGC 2 cut(s) 425, 752
HinP1I GCGC 2 cut(s) 425, 752
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 6 cut(s) 116, 160, 412, 1147, 1198, 1297
Hpy166II GTNNAC 4 cut(s) 216, 343, 562, 1440
Hpy188I TCNGA 4 cut(s) 273, 569, 795, 1302
Hpy188III TCNNGA 8 cut(s) 31, 155, 379, 388, 946, 1013, 1258, 1349
Hpy8I GTNNAC 4 cut(s) 216, 343, 562, 1440
Hpy99I CGWCG 7 cut(s) 71, 320, 331, 650, 664, 862, 1259
HpyAV CCTTC 9 cut(s) 493, 539, 688, 863, 1027, 1108, 1114, 1240, 1271
HpyCH4III ACNGT 4 cut(s) 16, 81, 106, 1020
HpyCH4IV ACGT 2 cut(s) 96, 676
HpyCH4V TGCA 2 cut(s) 621, 1400
HpyF10VI GCNNNNNNNGC 7 cut(s) 138, 197, 433, 480, 512, 808, 995
HpyF3I CTNAG 2 cut(s) 536, 1448
HpySE526I ACGT 2 cut(s) 96, 676
Hsp92I GRCGYC 2 cut(s) 66, 363
Hsp92II CATG 3 cut(s) 76, 214, 1016
HspAI GCGC 2 cut(s) 425, 752
Kpn2I TCCGGA 1 cut(s) 154
Kzo9I GATC 4 cut(s) 231, 268, 273, 1332
LmnI GCTCC 7 cut(s) 4, 188, 569, 781, 908, 915, 1319
Lsp1109I GCAGC 3 cut(s) 605, 1035, 1045
LweI GCATC 3 cut(s) 423, 654, 694
MaeI CTAG 1 cut(s) 912
MaeII ACGT 2 cut(s) 96, 676
MaeIII GTNAC 2 cut(s) 106, 628
MalI GATC 4 cut(s) 233, 270, 275, 1334
MbiI CCGCTC 1 cut(s) 360
MboI GATC 4 cut(s) 231, 268, 273, 1332
MboII GAAGA 7 cut(s) 46, 143, 838, 841, 1042, 1240, 1276
MfeI CAATTG 1 cut(s) 613
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 743
MlsI TGGCCA 1 cut(s) 1422
MluCI AATT 6 cut(s) 613, 655, 724, 786, 1286, 1323
MluNI TGGCCA 1 cut(s) 1422
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 886
Mox20I TGGCCA 1 cut(s) 1422
MroI TCCGGA 1 cut(s) 154
MroXI GAANNNNTTC 2 cut(s) 1235, 1250
MscI TGGCCA 1 cut(s) 1422
MseI TTAA 5 cut(s) 147, 654, 879, 1095, 1167
MslI CAYNNNNRTG 2 cut(s) 44, 675
Msp20I TGGCCA 1 cut(s) 1422
MspR9I CCNGG 4 cut(s) 59, 202, 1107, 1386
MunI CAATTG 1 cut(s) 613
Mva1269I GAATGC 1 cut(s) 1358
MvaI CCWGG 4 cut(s) 59, 202, 1107, 1386
MvnI CGCG 4 cut(s) 374, 417, 515, 639
MwoI GCNNNNNNNGC 7 cut(s) 138, 197, 433, 480, 512, 808, 995
NdeII GATC 4 cut(s) 231, 268, 273, 1332
NlaIII CATG 3 cut(s) 76, 214, 1016
NlaIV GGNNCC 5 cut(s) 6, 29, 571, 747, 783
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 106
NspI RCATGY 1 cut(s) 214
OliI CACNNNNGTG 2 cut(s) 44, 675
PaeR7I CTCGAG 1 cut(s) 397
PagI TCATGA 1 cut(s) 1012
PceI AGGCCT 1 cut(s) 403
PciI ACATGT 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 483
PctI GAATGC 1 cut(s) 1358
PdmI GAANNNNTTC 2 cut(s) 1235, 1250
PfeI GAWTC 5 cut(s) 160, 412, 1147, 1198, 1297
PflFI GACNNNGTC 2 cut(s) 374, 1081
PflMI CCANNNNNTGG 1 cut(s) 1429
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 677
PscI ACATGT 1 cut(s) 210
PshBI ATTAAT 1 cut(s) 1095
Psp6I CCWGG 4 cut(s) 57, 200, 1105, 1384
PspGI CCWGG 4 cut(s) 57, 200, 1105, 1384
PspN4I GGNNCC 5 cut(s) 6, 29, 571, 747, 783
PspPI GGNCC 1 cut(s) 559
PspXI VCTCGAGB 1 cut(s) 397
PsrI GAACNNNNNNTAC 2 cut(s) 197, 229
PsuI RGATCY 1 cut(s) 268
PsyI GACNNNGTC 2 cut(s) 374, 1081
RsaI GTAC 6 cut(s) 78, 215, 528, 851, 956, 1022
RsaNI GTAC 6 cut(s) 77, 214, 527, 850, 955, 1021
RseI CAYNNNNRTG 2 cut(s) 44, 675
SaqAI TTAA 5 cut(s) 147, 654, 879, 1095, 1167
Sau3AI GATC 4 cut(s) 231, 268, 273, 1332
Sau96I GGNCC 1 cut(s) 559
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 4 cut(s) 59, 202, 1107, 1386
SduI GDGCHC 1 cut(s) 743
SfaNI GCATC 3 cut(s) 423, 654, 694
Sfr274I CTCGAG 1 cut(s) 397
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 1 cut(s) 559
SlaI CTCGAG 1 cut(s) 397
SmiMI CAYNNNNRTG 2 cut(s) 44, 675
SmlI CTYRAG 2 cut(s) 397, 761
SmoI CTYRAG 2 cut(s) 397, 761
Sse9I AATT 6 cut(s) 613, 655, 724, 786, 1286, 1323
SseBI AGGCCT 1 cut(s) 403
SspMI CTAG 1 cut(s) 912
StuI AGGCCT 1 cut(s) 403
StyD4I CCNGG 4 cut(s) 57, 200, 1105, 1384
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 4 cut(s) 16, 81, 106, 1020
TaiI ACGT 2 cut(s) 99, 679
TasI AATT 6 cut(s) 613, 655, 724, 786, 1286, 1323
TatI WGTACW 2 cut(s) 213, 1020
TauI GCSGC 7 cut(s) 200, 363, 420, 483, 506, 518, 670
TfiI GAWTC 5 cut(s) 160, 412, 1147, 1198, 1297
Tru1I TTAA 5 cut(s) 147, 654, 879, 1095, 1167
Tru9I TTAA 5 cut(s) 147, 654, 879, 1095, 1167
TscAI CASTG 2 cut(s) 1430, 1481
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 3 cut(s) 618, 1048, 1058
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 7 cut(s) 581, 821, 1001, 1029, 1277, 1422, 1460
TspGWI ACGGA 3 cut(s) 544, 846, 1298
TspRI CASTG 2 cut(s) 1430, 1481
Tth111I GACNNNGTC 2 cut(s) 374, 1081
Van91I CCANNNNNTGG 1 cut(s) 1429
VpaK11BI GGWCC 1 cut(s) 559
VspI ATTAAT 1 cut(s) 1095
XapI RAATTY 2 cut(s) 724, 1286
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 810
XhoI CTCGAG 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 2 cut(s) 1235, 1250
XspI CTAG 1 cut(s) 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.