FvH4_6g10540

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013085
Physical Location & Seq
Forward (+)
6320216 .. 6322327
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g10540.t1

Sequence Viewer

Length: 1536 bp
ATGGAGCCTATTACTGCGAAGCCGGGGTTCCTCAAGAACACTCTGGTGCGGTGTTTCTTGTTCGGCGTTGTGCTGGTGCTGTTCCGGTTCGCTTACGTCATCACCGTGACCGGAGAGTCGTGTAGAGTTGGAAATTTCTGCTTCCTGAAAGAGAATCTCAACTTCGTCATCCCCGGCAGCGGCGCGTCGGCGGCGGTGGCGGCGAACGGCGCGGTTTCGACCGGTGCGGTGGGGCCGGAGAGGAAGGACGTGTACACCAGCAAGGACTGGATCAAGGCCGTCCATTTTTACTCCGCCGTTTTCCAAGATCTGATCTCGCAAGGGTTTCTCTCGCCGGAATCGAAGTCCTTGTGCGTGGAGACTCCGACGGGGCAGGAAGTTTACGCCTTGAAGGAGACCGGCGTGAAGAACGCCGTCGGGATTTTCAAGAAGGCGTCGAGGCCGTTGGTGGTTCGCGGCGAGGCGCACCGGATGCCGTTCGCCGATAACTCGTTCGACTTCGTTTTCTCCGGCAACGGGAGGCTGGACAAGTCGCCGAAGGCGGCGGAGTTCGCGGCGGAGATTGTTAGGACGCTCAGGCCCGAAGGGTTTGCGGTGGTCCATATCGGAGCCAAAGATACGTATAGCTTCCATTCTTTTATTGATTTGTTCAGTTGCTGTAAGTTAGTTACTTCACGCGACATCGACGGCTTTAATTCGTCGATGCCGCATATACGTGAGATGGTGTTGAAAAAGGAGTGTGATGCTTCTACTGAAGTGTCAAATTCCGGCGGAAGTTCCGGTAATGAGTGCTCGGTGCCGGGGCATAAGCTTGAGTTGGTGAGGAAGGCCGAGCCGATTATTCAAATCGAGCCGTTGAAGCCGTGGATTACATTGAAGAAGAATATACAGAATGTGAAGTATCTCCCTTCGATTGTGGATATTAGCTTTAAGAGGAGGTATGTTTATGTTGATGTTGGAGCTAGGAGCTATGGTTCGAGTATTGGGAGCTGGTTCAAGAAGCAGTACCCGAAGCAGAACAAGACTTTTGAAGTTTTCGCCATTGAGGCTGATAAGACTTTTCATGAGCAGTATAAGATGAAGAAGAGGATTACTTTGCTGCCGTATGCGGCGTGGGTGAGGAATGAGACATTGTCGTTTGAGATTAATGGAGACCCCGGGGAGAAGGTGAAGGAGAAGGGTAGGGGAATGGGGAGGATTCAGCCGGGGAAGTCGGCCAATGGAGGGTTTGATGGTCAGGTGGATAGGATTCAAGGGTTTGATTTTGCGGAGTGGGTGAAGAATACTTTCACAGAGAAGGATTTCGTGGTGATGAAGATGGATGTGGAAGGGACGGAATTTGATTTGATTCCGAGGCTGTTTGAGACCGGGGCAATTTGTTTGATCGATGAGGTGTTTCTTGAATGCCATTACAATAGGTGGCAGAGGACGCTCCCTGGTGAGAGGAGTGCAAAGTATGAGAAAACTTATGGCCAGTGCTTGGACTTGTTCACTTCTCTAAGGCAAAGTGGGGTTCTTGTTCATCAGTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

512

Amino Acids

57.33

Weight (kDa)

8.83

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 147 - 199 1.4e-07 Methyltransferase domain
DUF7870 PF25276 285 - 374 1.4e-10 Domain of unknown function (DUF7870)
Methyltransf_21 PF05050 318 - 473 9.2e-06 Methyltransferase FkbM domain
DUF7870 PF25276 417 - 511 1.1e-19 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01710
fragaria_vesca FvH4_6g10540
malus_domestica MD04G1167500.v1.1 MD12G1180800.v1.1
prunus_persica Prupe.6G285700_v2.0.a1
pyrus_communis pycom04g14860 pycom12g17040
rosa_chinensis RchiOBHm_Chr3g0461501
rosa_laevigata RLG00000024927
rosa_multiflora Rmu_sc0002677.1_g000002
rosa_roxburghii Rroxscaffold_6G00418230 Rroxscaffold_6G00418560
rosa_rugosa Rorug03G0051300
rosa_samantha Rh3AG109100 Rh3BG112900 Rh3CG114400 Rh3DG114500
rosa_wichuraiana Rw3G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 115
AccB1I GGYRCC 1 cut(s) 796
AccB7I CCANNNNNTGG 1 cut(s) 1480
AccII CGCG 5 cut(s) 185, 212, 456, 554, 678
AclWI GGATC 1 cut(s) 278
AcoI YGGCCR 2 cut(s) 1215, 1471
AcsI RAATTY 3 cut(s) 133, 763, 1337
AcuI CTGAAG 1 cut(s) 774
AcyI GRCGYC 1 cut(s) 434
AfaI GTAC 2 cut(s) 254, 1007
AfiI CCNNNNNNNGG 4 cut(s) 179, 516, 1224, 1480
AflIII ACRYGT 1 cut(s) 249
AgeI ACCGGT 1 cut(s) 221
AjiI CACGTC 1 cut(s) 250
AjnI CCWGG 1 cut(s) 1435
AleI CACNNNNGTG 1 cut(s) 44
AloI GAACNNNNNNTCC 2 cut(s) 958, 990
AluBI AGCT 6 cut(s) 627, 811, 927, 962, 969, 990
AluI AGCT 6 cut(s) 627, 811, 927, 962, 969, 990
Alw21I GWGCWC 1 cut(s) 794
Alw26I GTCTC 5 cut(s) 353, 389, 1121, 1146, 1358
AlwI GGATC 1 cut(s) 278
AlwNI CAGNNNCTG 1 cut(s) 657
Ama87I CYCGRG 1 cut(s) 1157
AoxI GGCC 7 cut(s) 233, 276, 440, 578, 828, 1215, 1471
ApeKI GCWGC 2 cut(s) 177, 1099
ApoI RAATTY 3 cut(s) 133, 763, 1337
AseI ATTAAT 1 cut(s) 1146
AsiGI ACCGGT 1 cut(s) 221
Asp700I GAANNNNTTC 2 cut(s) 1286, 1301
AspLEI GCGC 3 cut(s) 185, 212, 466
AspS9I GGNCC 3 cut(s) 233, 579, 598
AsuC2I CCSGG 7 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369
AsuHPI GGTGA 7 cut(s) 94, 832, 1129, 1180, 1288, 1321, 1451
AvaI CYCGRG 1 cut(s) 1157
AvaII GGWCC 1 cut(s) 598
BalI TGGCCA 1 cut(s) 1473
BanI GGYRCC 1 cut(s) 796
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
Bbv12I GWGCWC 1 cut(s) 794
BbvI GCAGC 2 cut(s) 189, 1086
BccI CCATC 3 cut(s) 715, 1226, 1312
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 1 cut(s) 1437
BcnI CCSGG 7 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369
BcoDI GTCTC 5 cut(s) 353, 389, 1121, 1146, 1358
BfaI CTAG 1 cut(s) 963
BglI GCCNNNNNGGC 1 cut(s) 1046
BglII AGATCT 1 cut(s) 307
Bme1390I CCNGG 8 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369, 1437
Bme18I GGWCC 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 1157
BmgBI CACGTC 1 cut(s) 250
BmgT120I GGNCC 3 cut(s) 233, 579, 598
BmiI GGNNCC 5 cut(s) 6, 29, 234, 610, 798
BmrFI CCNGG 8 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369, 1437
BmsI GCATC 3 cut(s) 462, 693, 733
Bpu10I CCTNAGC 1 cut(s) 575
BpuEI CTTGAG 2 cut(s) 17, 833
BpuMI CCSGG 7 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369
Bsa29I ATCGAT 1 cut(s) 1386
BsaAI YACGTR 2 cut(s) 621, 716
BsaHI GRCGYC 1 cut(s) 434
BsaI GGTCTC 3 cut(s) 389, 1146, 1358
BsaWI WCCGGW 5 cut(s) 84, 110, 221, 468, 779
BsaXI ACNNNNNCTCC 2 cut(s) 958, 988
Bsc4I CCNNNNNNNGG 4 cut(s) 179, 516, 1224, 1480
Bse118I RCCGGY 2 cut(s) 221, 398
Bse1I ACTGG 2 cut(s) 272, 1474
BseBI CCWGG 1 cut(s) 1437
BseCI ATCGAT 1 cut(s) 1386
BseGI GGATG 3 cut(s) 168, 477, 1327
BseLI CCNNNNNNNGG 4 cut(s) 179, 516, 1224, 1480
BseMII CTCAG 1 cut(s) 589
BseNI ACTGG 2 cut(s) 272, 1474
BseRI GAGGAG 2 cut(s) 949, 1459
BseXI GCAGC 2 cut(s) 189, 1086
Bsh1236I CGCG 5 cut(s) 185, 212, 456, 554, 678
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 7 cut(s) 235, 278, 442, 580, 830, 1217, 1473
BshNI GGYRCC 1 cut(s) 796
BshTI ACCGGT 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 1386
BsiEI CGRYCG 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 794
BsiHKCI CYCGRG 1 cut(s) 1157
BslFI GGGAC 1 cut(s) 1345
BslI CCNNNNNNNGG 4 cut(s) 179, 516, 1224, 1480
BsmAI GTCTC 5 cut(s) 353, 389, 1121, 1146, 1358
BsmFI GGGAC 1 cut(s) 1345
BsmI GAATGC 1 cut(s) 1409
BsnI GGCC 7 cut(s) 235, 278, 442, 580, 830, 1217, 1473
Bso31I GGTCTC 3 cut(s) 389, 1146, 1358
BsoBI CYCGRG 1 cut(s) 1157
Bsp1286I GDGCHC 1 cut(s) 794
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 4 cut(s) 270, 307, 312, 1383
BspANI GGCC 7 cut(s) 235, 278, 442, 580, 830, 1217, 1473
BspCNI CTCAG 1 cut(s) 588
BspDI ATCGAT 1 cut(s) 1386
BspFNI CGCG 5 cut(s) 185, 212, 456, 554, 678
BspHI TCATGA 1 cut(s) 1063
BspLI GGNNCC 5 cut(s) 6, 29, 234, 610, 798
BspPI GGATC 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 796
BspTNI GGTCTC 3 cut(s) 389, 1146, 1358
BsrFI RCCGGY 2 cut(s) 221, 398
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 2 cut(s) 272, 1474
BssAI RCCGGY 2 cut(s) 221, 398
BssMI GATC 4 cut(s) 270, 307, 312, 1383
BssNI GRCGYC 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 1437
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 1079
BstACI GRCGYC 1 cut(s) 434
BstAPI GCANNNNNTGC 1 cut(s) 472
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 2 cut(s) 621, 716
BstDEI CTNAG 2 cut(s) 575, 1499
BstDSI CCRYGG 1 cut(s) 863
BstF5I GGATG 3 cut(s) 168, 477, 1327
BstFNI CGCG 5 cut(s) 185, 212, 456, 554, 678
BstHHI GCGC 3 cut(s) 185, 212, 466
BstKTI GATC 4 cut(s) 273, 310, 315, 1386
BstMAI GTCTC 5 cut(s) 353, 389, 1121, 1146, 1358
BstMBI GATC 4 cut(s) 270, 307, 312, 1383
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 9 cut(s) 191, 197, 200, 209, 472, 551, 859, 1046, 1429
BstNI CCWGG 1 cut(s) 1437
BstSCI CCNGG 8 cut(s) 22, 172, 799, 1156, 1157, 1204, 1367, 1435
BstSNI TACGTA 1 cut(s) 621
BstUI CGCG 5 cut(s) 185, 212, 456, 554, 678
BstV1I GCAGC 2 cut(s) 189, 1086
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
Bsu15I ATCGAT 1 cut(s) 1386
BsuRI GGCC 7 cut(s) 235, 278, 442, 580, 830, 1217, 1473
BsuTUI ATCGAT 1 cut(s) 1386
BtgI CCRYGG 1 cut(s) 863
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 3 cut(s) 168, 477, 1327
BtsIMutI CAGTG 2 cut(s) 1481, 1532
CaiI CAGNNNCTG 1 cut(s) 657
CciI TCATGA 1 cut(s) 1063
CfoI GCGC 3 cut(s) 185, 212, 466
Cfr10I RCCGGY 2 cut(s) 221, 398
Cfr13I GGNCC 3 cut(s) 233, 579, 598
Cfr9I CCCGGG 1 cut(s) 1157
ClaI ATCGAT 1 cut(s) 1386
CseI GACGC 4 cut(s) 174, 423, 580, 1438
Csp6I GTAC 2 cut(s) 253, 1006
CspAI ACCGGT 1 cut(s) 221
CviAII CATG 1 cut(s) 1064
CviQI GTAC 2 cut(s) 253, 1006
DdeI CTNAG 2 cut(s) 575, 1499
DpnI GATC 4 cut(s) 272, 309, 314, 1385
DpnII GATC 4 cut(s) 270, 307, 312, 1383
DrdI GACNNNNNNGTC 1 cut(s) 115
DseDI GACNNNNNNGTC 1 cut(s) 115
EaeI YGGCCR 2 cut(s) 1215, 1471
Eam1104I CTCTTC 1 cut(s) 1079
EarI CTCTTC 1 cut(s) 1079
EciI GGCGGA 4 cut(s) 283, 560, 572, 786
Eco105I TACGTA 1 cut(s) 621
Eco31I GGTCTC 3 cut(s) 389, 1146, 1358
Eco47I GGWCC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 774
Eco88I CYCGRG 1 cut(s) 1157
EcoRII CCWGG 1 cut(s) 1435
FaeI CATG 1 cut(s) 1067
FaqI GGGAC 1 cut(s) 1345
FatI CATG 1 cut(s) 1063
FokI GGATG 3 cut(s) 155, 484, 1334
FspBI CTAG 1 cut(s) 963
GlaI GCGC 3 cut(s) 184, 211, 465
HaeIII GGCC 7 cut(s) 235, 278, 442, 580, 830, 1217, 1473
HgaI GACGC 4 cut(s) 174, 423, 580, 1438
HhaI GCGC 3 cut(s) 185, 212, 466
Hin1I GRCGYC 1 cut(s) 434
Hin1II CATG 1 cut(s) 1067
Hin6I GCGC 3 cut(s) 183, 210, 464
HinP1I GCGC 3 cut(s) 183, 210, 464
HindIII AAGCTT 1 cut(s) 809
HinfI GANTC 7 cut(s) 116, 154, 338, 361, 1198, 1249, 1348
HphI GGTGA 7 cut(s) 94, 832, 1129, 1180, 1288, 1321, 1451
Hpy166II GTNNAC 4 cut(s) 253, 255, 382, 1491
Hpy188I TCNGA 4 cut(s) 312, 366, 608, 1353
Hpy188III TCNNGA 7 cut(s) 34, 145, 418, 427, 997, 1064, 1400
Hpy8I GTNNAC 4 cut(s) 253, 255, 382, 1491
Hpy99I CGWCG 6 cut(s) 190, 370, 419, 439, 689, 703
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 4 cut(s) 96, 249, 620, 715
HpyCH4V TGCA 1 cut(s) 1451
HpyF10VI GCNNNNNNNGC 9 cut(s) 191, 197, 200, 209, 472, 551, 859, 1046, 1429
HpyF3I CTNAG 2 cut(s) 575, 1499
HpySE526I ACGT 4 cut(s) 96, 249, 620, 715
Hsp92I GRCGYC 1 cut(s) 434
Hsp92II CATG 1 cut(s) 1067
HspAI GCGC 3 cut(s) 183, 210, 464
Kzo9I GATC 4 cut(s) 270, 307, 312, 1383
LmnI GCTCC 6 cut(s) 4, 608, 959, 966, 987, 1437
Lsp1109I GCAGC 2 cut(s) 189, 1086
LweI GCATC 3 cut(s) 462, 693, 733
MaeI CTAG 1 cut(s) 963
MaeII ACGT 4 cut(s) 96, 249, 620, 715
MaeIII GTNAC 2 cut(s) 106, 667
MalI GATC 4 cut(s) 272, 309, 314, 1385
MboI GATC 4 cut(s) 270, 307, 312, 1383
MboII GAAGA 7 cut(s) 418, 889, 892, 1093, 1096, 1291, 1327
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 794
MlsI TGGCCA 1 cut(s) 1473
MluCI AATT 5 cut(s) 133, 694, 763, 1337, 1374
MluNI TGGCCA 1 cut(s) 1473
MlyI GAGTC 2 cut(s) 125, 355
MmeI TCCRAC 3 cut(s) 109, 389, 937
Mox20I TGGCCA 1 cut(s) 1473
MroXI GAANNNNTTC 2 cut(s) 1286, 1301
MscI TGGCCA 1 cut(s) 1473
MseI TTAA 3 cut(s) 693, 930, 1146
MslI CAYNNNNRTG 3 cut(s) 44, 104, 714
Msp20I TGGCCA 1 cut(s) 1473
MspA1I CMGCKG 1 cut(s) 180
MspR9I CCNGG 8 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369, 1437
Mva1269I GAATGC 1 cut(s) 1409
MvaI CCWGG 1 cut(s) 1437
MvnI CGCG 5 cut(s) 185, 212, 456, 554, 678
MwoI GCNNNNNNNGC 9 cut(s) 191, 197, 200, 209, 472, 551, 859, 1046, 1429
NciI CCSGG 7 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369
NdeII GATC 4 cut(s) 270, 307, 312, 1383
NlaIII CATG 1 cut(s) 1067
NlaIV GGNNCC 5 cut(s) 6, 29, 234, 610, 798
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 1 cut(s) 106
OliI CACNNNNGTG 1 cut(s) 44
PagI TCATGA 1 cut(s) 1063
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 338
PctI GAATGC 1 cut(s) 1409
PdmI GAANNNNTTC 2 cut(s) 1286, 1301
PfeI GAWTC 5 cut(s) 154, 338, 1198, 1249, 1348
PflFI GACNNNGTC 1 cut(s) 1132
PflMI CCANNNNNTGG 1 cut(s) 1480
PinAI ACCGGT 1 cut(s) 221
PleI GAGTC 2 cut(s) 124, 355
PpsI GAGTC 2 cut(s) 124, 355
Ppu21I YACGTR 2 cut(s) 621, 716
PshBI ATTAAT 1 cut(s) 1146
Psp6I CCWGG 1 cut(s) 1435
PspGI CCWGG 1 cut(s) 1435
PspN4I GGNNCC 5 cut(s) 6, 29, 234, 610, 798
PspPI GGNCC 3 cut(s) 233, 579, 598
PstNI CAGNNNCTG 1 cut(s) 657
PsuI RGATCY 1 cut(s) 307
PsyI GACNNNGTC 1 cut(s) 1132
RsaI GTAC 2 cut(s) 254, 1007
RsaNI GTAC 2 cut(s) 253, 1006
RseI CAYNNNNRTG 3 cut(s) 44, 104, 714
SaqAI TTAA 3 cut(s) 693, 930, 1146
Sau3AI GATC 4 cut(s) 270, 307, 312, 1383
Sau96I GGNCC 3 cut(s) 233, 579, 598
SchI GAGTC 2 cut(s) 125, 355
ScrFI CCNGG 8 cut(s) 24, 174, 801, 1158, 1159, 1206, 1369, 1437
SduI GDGCHC 1 cut(s) 794
SfaNI GCATC 3 cut(s) 462, 693, 733
SinI GGWCC 1 cut(s) 598
SmaI CCCGGG 1 cut(s) 1159
SmiMI CAYNNNNRTG 3 cut(s) 44, 104, 714
SmlI CTYRAG 2 cut(s) 32, 812
SmoI CTYRAG 2 cut(s) 32, 812
SnaBI TACGTA 1 cut(s) 621
Sse9I AATT 5 cut(s) 133, 694, 763, 1337, 1374
SspMI CTAG 1 cut(s) 963
StyD4I CCNGG 8 cut(s) 22, 172, 799, 1156, 1157, 1204, 1367, 1435
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 4 cut(s) 99, 252, 623, 718
TasI AATT 5 cut(s) 133, 694, 763, 1337, 1374
TatI WGTACW 1 cut(s) 252
TauI GCSGC 8 cut(s) 183, 194, 203, 459, 545, 557, 709, 1112
TfiI GAWTC 5 cut(s) 154, 338, 1198, 1249, 1348
Tru1I TTAA 3 cut(s) 693, 930, 1146
Tru9I TTAA 3 cut(s) 693, 930, 1146
TscAI CASTG 2 cut(s) 1481, 1532
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 177, 1099
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 4 cut(s) 1052, 1094, 1328, 1511
TspGWI ACGGA 1 cut(s) 1349
TspMI CCCGGG 1 cut(s) 1157
TspRI CASTG 2 cut(s) 1481, 1532
Tth111I GACNNNGTC 1 cut(s) 1132
Van91I CCANNNNNTGG 1 cut(s) 1480
VpaK11BI GGWCC 1 cut(s) 598
VspI ATTAAT 1 cut(s) 1146
XapI RAATTY 3 cut(s) 133, 763, 1337
XmaI CCCGGG 1 cut(s) 1157
XmnI GAANNNNTTC 2 cut(s) 1286, 1301
XspI CTAG 1 cut(s) 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.