pycom04g14860

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013085
Physical Location & Seq
Reverse (-)
17635047 .. 17636621
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g14860.1

Sequence Viewer

Length: 1575 bp
ATGGAACCCGGCTCCGCCGCCGTTGGCAAGCCCGGTCTCCTTAAGAACGTTTTTATACGGTGTTTTTTGTTCGGCGTTTTGATTGTTCTCTGCCGCTTCGCCTACGTCGTCACCGTCACCGGCGAGTCGTGTGAGCTCGGCAACTTCTGCTTCTTCTCTCTCCCGGAAAATCTTAACTTCGTTATAGCGAAGACCGGCAGTGCGGCCATCGCCGCCAAAAACGACGCCGTGCCATCGAGGTCGGCGGGGTCCTCGAAGCCCGATCTCTACACCAGCAAGGACTGGATCCAGGCCGTCCATTTCTACGCCGCCGTTTTCCAGGATCTCATGTCCCAGGGCTTCCTCTCCCGGAAAGCCAAGTCGCTCTGCGTCGAGACCCCCTCCGGTCACGACGTCTACGCCCTGAGAGAGTCCGGCGTCAAGGACGCGGTCGGTGTTTTTAAGAAAGCGTTGCGTCCTTTGGTAATTCCGGGCGAAGCGCACCGTTTACCGTTTGCTGACAACAGCTTTGACTTTGTTTTCTCCGGCGGCGGACGTCTCGACAAGTCGCCAAAGCCGGCGGATTTCGCCGCCGAAATTGTGCGGACGCTGAAGCCCGAAGGTTTTGCGGTGGTCCACGTGGGCGCGAGAGACACTTACAGCTTCCATTCGTTTATTGAATTGTTTAACTGCAGCAAGCTCGTGACTTCGCGCGACATCGACGGCTTCAATTCGTCGATGCCAAAGATTCGCGAGATGGTTTTGAGGAAGGAGTGCGGCGGCGGCGGCGGCGAGATTGAGGGTTTAGGCCACAGAGTCGAGAACCCTGGTGGCAGTGTTGGTGCTAAGTGCTCGGTTCCTGAGCATAAGATGGAGATGGTGAGAAAGGCGGAGCCTTTGATTGAAATAGAGCCGTTGAAGCCGTGGATTACTCTGAAGAAGAATATACAGAACGTTAAGTACCTGCCAACAATGGCGGATATAAGCTTCAAGAAGCGGTATGCTTACGTGGATGTCGGAGCTCGGAGCTATGGCTCCAGCATTGGCAGCTGGTTCAAGAAGCAGTACCCGAAACAGAACAAGAGTTTCGAAGTTTTCGCCATTGAAGCCGATAAGACATTCCATGATCAGTACAAGTTGAAGAAGGGGGTCACATTGCTACCTTATGCAGCTTGGGTGAGGAATGAAACATTGTCTTTCGAGATCAATAGCGACCCGGGTGAGAAGGTGAAGGAGAAAGGCAGAGGAATGGGGAGGATTCAGCCGGCAAAGGCGGCCAATGGCGGATTTAACAGAGAGGTGGATCAGATTCAGGGGTTTGATTTTGCGAATTGGTTGAAGAAGACCTTTTCGGAGAAGGATTTCGTGGTGATGAAGATGGATGTGGAAGGGACGGAATTCGATTTGATTCCGAGGCTGTTTGAGACCGGAGCAATCTGTTTGATCGACGAGATATTTCTCGAGTGCCATTACAACCGGTGGCAGAGGTGTTGCCCCGGCGAGAGGAGTTCGAAGTATGAGAAGAGTTATGGTCAGTGCTTGGACCTGTTTGCTTCACTGAGACAAAGTGGTGTTCTTGTTCATCAATGGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

525

Amino Acids

58.46

Weight (kDa)

8.83

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 155 - 204 7.5e-08 Methyltransferase domain
DUF7870 PF25276 300 - 375 4.5e-09 Domain of unknown function (DUF7870)
DUF7870 PF25276 428 - 524 1.6e-20 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01710
fragaria_vesca FvH4_6g10540
malus_domestica MD04G1167500.v1.1 MD12G1180800.v1.1
prunus_persica Prupe.6G285700_v2.0.a1
pyrus_communis pycom04g14860 pycom12g17040
rosa_chinensis RchiOBHm_Chr3g0461501
rosa_laevigata RLG00000024927
rosa_multiflora Rmu_sc0002677.1_g000002
rosa_roxburghii Rroxscaffold_6G00418230 Rroxscaffold_6G00418560
rosa_rugosa Rorug03G0051300
rosa_samantha Rh3AG109100 Rh3BG112900 Rh3CG114400 Rh3DG114500
rosa_wichuraiana Rw3G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 396, 538
Acc36I ACCTGC 1 cut(s) 951
AccI GTMKAC 1 cut(s) 396
AccII CGCG 5 cut(s) 428, 626, 691, 693, 732
AclI AACGTT 2 cut(s) 48, 933
AclWI GGATC 4 cut(s) 280, 293, 330, 1290
AcoI YGGCCR 2 cut(s) 204, 1254
AcsI RAATTY 1 cut(s) 1376
AcuI CTGAAG 2 cut(s) 611, 935
AcvI CACGTG 1 cut(s) 619
AcyI GRCGYC 4 cut(s) 225, 393, 417, 535
AfaI GTAC 3 cut(s) 941, 1046, 1112
AfiI CCNNNNNNNGG 1 cut(s) 1482
AflII CTTAAG 1 cut(s) 41
AgeI ACCGGT 1 cut(s) 1455
AgsI TTSAA 9 cut(s) 659, 709, 884, 898, 970, 1036, 1085, 1120, 1318
AjnI CCWGG 4 cut(s) 288, 318, 333, 805
AluBI AGCT 9 cut(s) 136, 507, 642, 679, 966, 1001, 1008, 1029, 1151
AluI AGCT 9 cut(s) 136, 507, 642, 679, 966, 1001, 1008, 1029, 1151
Alw21I GWGCWC 3 cut(s) 138, 833, 1003
Alw26I GTCTC 6 cut(s) 41, 368, 542, 624, 1397, 1534
AlwI GGATC 4 cut(s) 280, 293, 330, 1290
Ama87I CYCGRG 2 cut(s) 1195, 1439
AoxI GGCC 4 cut(s) 204, 291, 787, 1254
ApeKI GCWGC 3 cut(s) 672, 1026, 1148
ApoI RAATTY 1 cut(s) 1376
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
AsiGI ACCGGT 1 cut(s) 1455
Asp700I GAANNNNTTC 1 cut(s) 1340
AspLEI GCGC 3 cut(s) 481, 626, 693
AspS9I GGNCC 3 cut(s) 249, 613, 1522
AsuC2I CCSGG 8 cut(s) 9, 33, 164, 349, 471, 1196, 1197, 1476
AsuHPI GGTGA 7 cut(s) 103, 109, 871, 1168, 1211, 1219, 1360
AsuII TTCGAA 2 cut(s) 1068, 1490
AvaI CYCGRG 2 cut(s) 1195, 1439
AvaII GGWCC 3 cut(s) 249, 613, 1522
BamHI GGATCC 1 cut(s) 285
BanII GRGCYC 2 cut(s) 138, 1003
BauI CACGAG 1 cut(s) 680
BbrPI CACGTG 1 cut(s) 619
BbsI GAAGAC 2 cut(s) 197, 1328
Bbv12I GWGCWC 3 cut(s) 138, 833, 1003
BbvI GCAGC 3 cut(s) 684, 1038, 1160
BccI CCATC 6 cut(s) 215, 241, 730, 844, 850, 1351
BceAI ACGGC 7 cut(s) 5, 212, 278, 296, 718, 877, 886
BcgI CGANNNNNNTGC 4 cut(s) 587, 621, 661, 695
BciT130I CCWGG 4 cut(s) 290, 320, 335, 807
BclI TGATCA 1 cut(s) 1105
BcnI CCSGG 8 cut(s) 9, 33, 164, 349, 471, 1196, 1197, 1476
BcoDI GTCTC 6 cut(s) 41, 368, 542, 624, 1397, 1534
BfmI CTRYAG 1 cut(s) 670
BfrI CTTAAG 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 951
Bme18I GGWCC 3 cut(s) 249, 613, 1522
BmeT110I CYCGRG 2 cut(s) 1195, 1439
BmgT120I GGNCC 3 cut(s) 249, 613, 1522
BmiI GGNNCC 7 cut(s) 6, 13, 250, 287, 837, 873, 1015
BmsI GCATC 1 cut(s) 708
BpiI GAAGAC 2 cut(s) 197, 1328
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpmI CTGGAG 1 cut(s) 1000
Bpu10I CCTNAGC 1 cut(s) 840
Bpu14I TTCGAA 2 cut(s) 1068, 1490
BpuMI CCSGG 8 cut(s) 9, 33, 164, 349, 471, 1196, 1197, 1476
BsaAI YACGTR 2 cut(s) 619, 988
BsaHI GRCGYC 4 cut(s) 225, 393, 417, 535
BsaI GGTCTC 3 cut(s) 41, 368, 1397
BsaJI CCNNGG 7 cut(s) 333, 334, 805, 902, 1195, 1391, 1474
BsaWI WCCGGW 3 cut(s) 383, 1406, 1455
Bsc4I CCNNNNNNNGG 1 cut(s) 1482
Bse118I RCCGGY 5 cut(s) 119, 194, 556, 1243, 1455
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 1133
BseBI CCWGG 4 cut(s) 290, 320, 335, 807
BseDI CCNNGG 7 cut(s) 333, 334, 805, 902, 1195, 1391, 1474
BseGI GGATG 2 cut(s) 997, 1366
BseLI CCNNNNNNNGG 1 cut(s) 1482
BseMI GCAATG 1 cut(s) 1133
BseMII CTCAG 3 cut(s) 395, 831, 1529
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 1498
BseXI GCAGC 3 cut(s) 684, 1038, 1160
Bsh1236I CGCG 5 cut(s) 428, 626, 691, 693, 732
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 206, 293, 789, 1256
BshTI ACCGGT 1 cut(s) 1455
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 3 cut(s) 138, 833, 1003
BsiHKCI CYCGRG 2 cut(s) 1195, 1439
BslFI GGGAC 2 cut(s) 316, 1384
BslI CCNNNNNNNGG 1 cut(s) 1482
BsmAI GTCTC 6 cut(s) 41, 368, 542, 624, 1397, 1534
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 2 cut(s) 316, 1384
BsnI GGCC 4 cut(s) 206, 293, 789, 1256
Bso31I GGTCTC 3 cut(s) 41, 368, 1397
BsoBI CYCGRG 2 cut(s) 1195, 1439
Bsp119I TTCGAA 2 cut(s) 1068, 1490
Bsp1286I GDGCHC 3 cut(s) 138, 833, 1003
Bsp143I GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
Bsp68I TCGCGA 1 cut(s) 732
BspANI GGCC 4 cut(s) 206, 293, 789, 1256
BspCNI CTCAG 3 cut(s) 396, 832, 1530
BspFNI CGCG 5 cut(s) 428, 626, 691, 693, 732
BspLI GGNNCC 7 cut(s) 6, 13, 250, 287, 837, 873, 1015
BspMAI CTGCAG 1 cut(s) 674
BspMI ACCTGC 1 cut(s) 951
BspPI GGATC 4 cut(s) 280, 293, 330, 1290
BspT104I TTCGAA 2 cut(s) 1068, 1490
BspTI CTTAAG 1 cut(s) 41
BspTNI GGTCTC 3 cut(s) 41, 368, 1397
BsrDI GCAATG 1 cut(s) 1133
BsrFI RCCGGY 5 cut(s) 119, 194, 556, 1243, 1455
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 5 cut(s) 119, 194, 556, 1243, 1455
BssECI CCNNGG 7 cut(s) 333, 334, 805, 902, 1195, 1391, 1474
BssMI GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
BssNI GRCGYC 4 cut(s) 225, 393, 417, 535
BssSI CACGAG 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 680
Bst2UI CCWGG 4 cut(s) 290, 320, 335, 807
Bst4CI ACNGT 4 cut(s) 60, 115, 485, 492
Bst6I CTCTTC 1 cut(s) 1496
BstACI GRCGYC 4 cut(s) 225, 393, 417, 535
BstAFI CTTAAG 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 147
BstBAI YACGTR 2 cut(s) 619, 988
BstBI TTCGAA 2 cut(s) 1068, 1490
BstC8I GCNNGC 4 cut(s) 29, 558, 677, 1245
BstDEI CTNAG 4 cut(s) 404, 825, 840, 1538
BstDSI CCRYGG 1 cut(s) 902
BstF5I GGATG 2 cut(s) 997, 1366
BstFNI CGCG 5 cut(s) 428, 626, 691, 693, 732
BstHHI GCGC 3 cut(s) 481, 626, 693
BstKTI GATC 7 cut(s) 265, 288, 325, 1108, 1185, 1285, 1425
BstMAI GTCTC 6 cut(s) 41, 368, 542, 624, 1397, 1534
BstMBI GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
BstMCI CGRYCG 1 cut(s) 432
BstNI CCWGG 4 cut(s) 290, 320, 335, 807
BstSFI CTRYAG 1 cut(s) 670
BstUI CGCG 5 cut(s) 428, 626, 691, 693, 732
BstV1I GCAGC 3 cut(s) 684, 1038, 1160
BstV2I GAAGAC 2 cut(s) 197, 1328
BstX2I RGATCY 2 cut(s) 285, 322
BstYI RGATCY 2 cut(s) 285, 322
BsuRI GGCC 4 cut(s) 206, 293, 789, 1256
BtgI CCRYGG 1 cut(s) 902
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 997, 1366
BtsI GCAGTG 2 cut(s) 205, 820
BtsIMutI CAGTG 4 cut(s) 205, 820, 1520, 1535
BtuMI TCGCGA 1 cut(s) 732
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 4 cut(s) 29, 558, 677, 1245
CfoI GCGC 3 cut(s) 481, 626, 693
Cfr10I RCCGGY 5 cut(s) 119, 194, 556, 1243, 1455
Cfr13I GGNCC 3 cut(s) 249, 613, 1522
Cfr9I CCCGGG 1 cut(s) 1195
CseI GACGC 6 cut(s) 233, 358, 406, 434, 443, 595
Csp6I GTAC 3 cut(s) 940, 1045, 1111
CspAI ACCGGT 1 cut(s) 1455
CviAII CATG 2 cut(s) 328, 1103
CviQI GTAC 3 cut(s) 940, 1045, 1111
DdeI CTNAG 4 cut(s) 404, 825, 840, 1538
DpnI GATC 7 cut(s) 264, 287, 324, 1107, 1184, 1284, 1424
DpnII GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
EaeI YGGCCR 2 cut(s) 204, 1254
Eam1104I CTCTTC 1 cut(s) 1496
EarI CTCTTC 1 cut(s) 1496
EciI GGCGGA 6 cut(s) 4, 546, 575, 884, 971, 1278
Ecl136II GAGCTC 2 cut(s) 136, 1001
Eco24I GRGCYC 2 cut(s) 138, 1003
Eco31I GGTCTC 3 cut(s) 41, 368, 1397
Eco47I GGWCC 3 cut(s) 249, 613, 1522
Eco53kI GAGCTC 2 cut(s) 136, 1001
Eco57I CTGAAG 2 cut(s) 611, 935
Eco72I CACGTG 1 cut(s) 619
Eco88I CYCGRG 2 cut(s) 1195, 1439
EcoICRI GAGCTC 2 cut(s) 136, 1001
EcoO109I RGGNCCY 1 cut(s) 249
EcoRI GAATTC 1 cut(s) 1376
EcoRII CCWGG 4 cut(s) 288, 318, 333, 805
EcoT38I GRGCYC 2 cut(s) 138, 1003
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 2 cut(s) 331, 1106
FalI AAGNNNNNCTT 2 cut(s) 1310, 1342
FaqI GGGAC 2 cut(s) 316, 1384
FatI CATG 2 cut(s) 327, 1102
FauI CCCGC 1 cut(s) 238
FbaI TGATCA 1 cut(s) 1105
FblI GTMKAC 1 cut(s) 396
FokI GGATG 2 cut(s) 1004, 1373
FriOI GRGCYC 2 cut(s) 138, 1003
GlaI GCGC 3 cut(s) 480, 625, 692
GsuI CTGGAG 1 cut(s) 1000
HaeIII GGCC 4 cut(s) 206, 293, 789, 1256
HgaI GACGC 6 cut(s) 233, 358, 406, 434, 443, 595
HhaI GCGC 3 cut(s) 481, 626, 693
Hin1I GRCGYC 4 cut(s) 225, 393, 417, 535
Hin1II CATG 2 cut(s) 331, 1106
Hin6I GCGC 3 cut(s) 479, 624, 691
HinP1I GCGC 3 cut(s) 479, 624, 691
HindIII AAGCTT 1 cut(s) 964
HinfI GANTC 7 cut(s) 125, 410, 727, 795, 1237, 1288, 1387
HphI GGTGA 7 cut(s) 103, 109, 871, 1168, 1211, 1219, 1360
Hpy166II GTNNAC 3 cut(s) 397, 488, 616
Hpy188I TCNGA 6 cut(s) 915, 998, 1005, 1287, 1333, 1392
Hpy8I GTNNAC 3 cut(s) 397, 488, 616
Hpy99I CGWCG 7 cut(s) 110, 227, 374, 395, 704, 718, 1430
HpyAV CCTTC 7 cut(s) 593, 742, 1117, 1198, 1204, 1330, 1361
HpyCH4III ACNGT 4 cut(s) 60, 115, 485, 492
HpyCH4IV ACGT 7 cut(s) 48, 105, 393, 535, 618, 933, 987
HpyCH4V TGCA 2 cut(s) 672, 1148
HpyF3I CTNAG 4 cut(s) 404, 825, 840, 1538
HpySE526I ACGT 7 cut(s) 48, 105, 393, 535, 618, 933, 987
Hsp92I GRCGYC 4 cut(s) 225, 393, 417, 535
Hsp92II CATG 2 cut(s) 331, 1106
HspAI GCGC 3 cut(s) 479, 624, 691
KroI GCCGGC 2 cut(s) 556, 1243
KroNI GCCGGC 2 cut(s) 558, 1245
Ksp22I TGATCA 1 cut(s) 1105
Kzo9I GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
LmnI GCTCC 6 cut(s) 17, 871, 998, 1005, 1019, 1409
Lsp1109I GCAGC 3 cut(s) 684, 1038, 1160
LweI GCATC 1 cut(s) 708
MaeII ACGT 7 cut(s) 48, 105, 393, 535, 618, 933, 987
MaeIII GTNAC 5 cut(s) 109, 115, 386, 682, 1129
MalI GATC 7 cut(s) 264, 287, 324, 1107, 1184, 1284, 1424
MboI GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
MboII GAAGA 9 cut(s) 145, 202, 928, 931, 1132, 1330, 1333, 1366, 1513
MflI RGATCY 2 cut(s) 285, 322
MhlI GDGCHC 3 cut(s) 138, 833, 1003
MluCI AATT 6 cut(s) 465, 576, 659, 709, 1309, 1376
MlyI GAGTC 3 cut(s) 134, 419, 804
MmeI TCCRAC 1 cut(s) 976
MroNI GCCGGC 2 cut(s) 556, 1243
MroXI GAANNNNTTC 1 cut(s) 1340
MseI TTAA 6 cut(s) 42, 174, 441, 666, 936, 1269
MspA1I CMGCKG 1 cut(s) 1029
MspCI CTTAAG 1 cut(s) 41
MvaI CCWGG 4 cut(s) 290, 320, 335, 807
MvnI CGCG 5 cut(s) 428, 626, 691, 693, 732
NaeI GCCGGC 2 cut(s) 558, 1245
NciI CCSGG 8 cut(s) 9, 33, 164, 349, 471, 1196, 1197, 1476
NdeII GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
NgoMIV GCCGGC 2 cut(s) 556, 1243
NlaIII CATG 2 cut(s) 331, 1106
NlaIV GGNNCC 7 cut(s) 6, 13, 250, 287, 837, 873, 1015
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 5 cut(s) 109, 115, 386, 682, 1129
NruI TCGCGA 1 cut(s) 732
NspV TTCGAA 2 cut(s) 1068, 1490
PaeR7I CTCGAG 1 cut(s) 1439
PasI CCCWGGG 1 cut(s) 334
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 111
PdiI GCCGGC 2 cut(s) 558, 1245
PdmI GAANNNNTTC 1 cut(s) 1340
PfeI GAWTC 4 cut(s) 727, 1237, 1288, 1387
PflFI GACNNNGTC 1 cut(s) 428
PfoI TCCNGGA 3 cut(s) 162, 318, 347
PinAI ACCGGT 1 cut(s) 1455
PleI GAGTC 3 cut(s) 133, 418, 803
PmaCI CACGTG 1 cut(s) 619
PmlI CACGTG 1 cut(s) 619
PpsI GAGTC 3 cut(s) 133, 418, 803
Ppu21I YACGTR 2 cut(s) 619, 988
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 138, 1003
Psp1406I AACGTT 2 cut(s) 48, 933
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 4 cut(s) 288, 318, 333, 805
PspCI CACGTG 1 cut(s) 619
PspGI CCWGG 4 cut(s) 288, 318, 333, 805
PspN4I GGNNCC 7 cut(s) 6, 13, 250, 287, 837, 873, 1015
PspPI GGNCC 3 cut(s) 249, 613, 1522
PspPPI RGGWCCY 1 cut(s) 249
PsrI GAACNNNNNNTAC 2 cut(s) 923, 955
PstI CTGCAG 1 cut(s) 674
PsuI RGATCY 2 cut(s) 285, 322
PsyI GACNNNGTC 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 1029
RruI TCGCGA 1 cut(s) 732
RsaI GTAC 3 cut(s) 941, 1046, 1112
RsaNI GTAC 3 cut(s) 940, 1045, 1111
SacI GAGCTC 2 cut(s) 138, 1003
SaqAI TTAA 6 cut(s) 42, 174, 441, 666, 936, 1269
Sau3AI GATC 7 cut(s) 262, 285, 322, 1105, 1182, 1282, 1422
Sau96I GGNCC 3 cut(s) 249, 613, 1522
SchI GAGTC 3 cut(s) 134, 419, 804
SduI GDGCHC 3 cut(s) 138, 833, 1003
SfaNI GCATC 1 cut(s) 708
SfcI CTRYAG 1 cut(s) 670
Sfr274I CTCGAG 1 cut(s) 1439
SfuI TTCGAA 2 cut(s) 1068, 1490
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 3 cut(s) 249, 613, 1522
SlaI CTCGAG 1 cut(s) 1439
SmaI CCCGGG 1 cut(s) 1197
SmlI CTYRAG 2 cut(s) 41, 1439
SmoI CTYRAG 2 cut(s) 41, 1439
Sse9I AATT 6 cut(s) 465, 576, 659, 709, 1309, 1376
SstI GAGCTC 2 cut(s) 138, 1003
TaaI ACNGT 4 cut(s) 60, 115, 485, 492
TaiI ACGT 7 cut(s) 51, 108, 396, 538, 621, 936, 990
TasI AATT 6 cut(s) 465, 576, 659, 709, 1309, 1376
TatI WGTACW 1 cut(s) 1110
TfiI GAWTC 4 cut(s) 727, 1237, 1288, 1387
Tru1I TTAA 6 cut(s) 42, 174, 441, 666, 936, 1269
Tru9I TTAA 6 cut(s) 42, 174, 441, 666, 936, 1269
TscAI CASTG 4 cut(s) 205, 820, 1520, 1542
TseFI GTSAC 5 cut(s) 109, 115, 386, 682, 1129
TseI GCWGC 3 cut(s) 672, 1026, 1148
Tsp45I GTSAC 5 cut(s) 109, 115, 386, 682, 1129
TspDTI ATGAA 3 cut(s) 1179, 1367, 1550
TspGWI ACGGA 1 cut(s) 1388
TspMI CCCGGG 1 cut(s) 1195
TspRI CASTG 4 cut(s) 205, 820, 1520, 1542
Tth111I GACNNNGTC 1 cut(s) 428
Vha464I CTTAAG 1 cut(s) 41
VpaK11BI GGWCC 3 cut(s) 249, 613, 1522
XapI RAATTY 1 cut(s) 1376
XhoI CTCGAG 1 cut(s) 1439
XmaI CCCGGG 1 cut(s) 1195
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 1340
ZraI GACGTC 2 cut(s) 394, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.