MD04G1167500.v1.1

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013085
Physical Location & Seq
Reverse (-)
25775112 .. 25776804
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1167500.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGGAACCCGGCTCCGCCGCCGCAGGCAAGCCCGGCCTCCTTAAGAACGTTTTTATACGGTGTTTTTTGTTCGGCGTTTTGATTGTTCTCTGCCGCTTCGCCTACGTCGTCACCGTCACCGGCGAGTCGTGTGAGCTCGGCAACTTCTGCTTCTTCTCTCTCCCGGAAAATCTTAACTTCGTTATAGCGAATACCGGCAGTGCGGCCATCGCCGCCAAAAACGACGCCGTGCCATCGAGGTCGGCGAGGTCCTTGAAGCCCGATCTCTACACCAGCAAGGACTGGATCCAGGCCGTCCATTTCTTCTCCGCCGTTTTCCAGGATCTCATGTCCCAGGGCTTCCTCTCCCGGAAAGCCAAGTCGCTCTGCGTCGAGACCCCCTCCGGGCACGACGTCTACGCCCTGAGAGAGTCCGGCGTCAAGGACGCGGTCGGTGTTTTTAAGAAAGCGTTGCGTCCCTTGGTAATTCCGGGCGAAGCGCACCGTTTACCGTTTGCTGACAACAGCTTTGACTTTGTTTTCTCTGGCGGCGGACGTCTCGACAAGTCGCCAAAGCCGGCGGATTTCGCCGCCGAAATCGTGCGGACGCTGAAGCCCGAAGGGTTTGCGGTGGTCCACGTGGGCGCGAGAGACACTTACAGCTTCCATTCGTTTATTGAATTGTTCAATTGCAGCAAGCTCGTGACTTCGCGCGACATCGACGGCTTCAATTCGTCGATGCCAAAGGTTCGCGAGATGGTTTTGAGGAAGGAGTGCGGCAGCGGCGGCGAGATTGAGGGTTTAGGCCACAGAGTCGAGAACCCTGGTGGCAGTGTTGGTGCTAAGTGCTCGGTTCCTGAGCATAAGATGGAGCTGGTGAGAAAGGCGGAGCCTTTGATTGAAATAGAGCCGTTGAAGCCGTGGATTACTCTGAAGAAGAATATACAGAACGTTAAGTACCTGCCAACAATGGCGGATATAAGCTTCAAGAAGCGATATGCTTACGTGGATGTCGGAGCTCGGAGCTATGGCTCCAGCATTGGCAGCTGGTTCAAGAAGCAGTACCCGAAACAGAACAAGACTTTCGAAGTTTTCGCCATTGAAGCCGATAAGACTTTCCATGATCAGTACAAGTTGAAGAAGGGGGTCACATTGCTACCTTATGCAGCTTGGGTGAGGAATGAAACGTTGTCTTTCGAGATCAATGGCGACCCGGGTGAGAAGGTGACGGAGAAAGGCAGAGGAATGGGGAGGATTCAGCCGGCAAAGGCGGCCAATGGCGGATTTAACAGAGAGGTGGATCAGATTCAGGGGTTTGATTTTGCGAATTGGTTGAAGAAGACCTTTTCGGAGAAGGATTTCGTGGTGATGAAGATGGATGTTGAAGGGACGGAATTCGATTTGATTCCGAGGCTGTTTGAGACCGGGGCAATCTGTTTGATCGACGAGATATTTCTCGAGTGCCATTACAACCGGTGGCAGAGGTGTTGCCCCGGGGAGCGGAGTTCGAAGTATGAGAAGAGTTATGGCCAGTGCTTGGACCTGTTTGCTTCACTGAGACAAAGTGGTGTTCTTGTTCATCAATGGTGGAAAAACTTTAGAATTTGTTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

532

Amino Acids

59.43

Weight (kDa)

8.88

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 155 - 204 7.7e-08 Methyltransferase domain
DUF7870 PF25276 299 - 374 4.8e-09 Domain of unknown function (DUF7870)
DUF7870 PF25276 427 - 523 1.7e-20 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01710
fragaria_vesca FvH4_6g10540
malus_domestica MD04G1167500.v1.1 MD12G1180800.v1.1
prunus_persica Prupe.6G285700_v2.0.a1
pyrus_communis pycom04g14860 pycom12g17040
rosa_chinensis RchiOBHm_Chr3g0461501
rosa_laevigata RLG00000024927
rosa_multiflora Rmu_sc0002677.1_g000002
rosa_roxburghii Rroxscaffold_6G00418230 Rroxscaffold_6G00418560
rosa_rugosa Rorug03G0051300
rosa_samantha Rh3AG109100 Rh3BG112900 Rh3CG114400 Rh3DG114500
rosa_wichuraiana Rw3G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 396, 538
Acc36I ACCTGC 1 cut(s) 948
AccB7I CCANNNNNTGG 1 cut(s) 1516
AccBSI CCGCTC 1 cut(s) 1480
AccI GTMKAC 1 cut(s) 396
AccII CGCG 5 cut(s) 428, 626, 691, 693, 732
AclI AACGTT 3 cut(s) 48, 930, 1166
AclWI GGATC 4 cut(s) 280, 293, 330, 1287
AcoI YGGCCR 3 cut(s) 204, 1251, 1507
AcsI RAATTY 2 cut(s) 1373, 1581
AcuI CTGAAG 2 cut(s) 611, 932
AcvI CACGTG 1 cut(s) 619
AcyI GRCGYC 4 cut(s) 225, 393, 417, 535
AfaI GTAC 3 cut(s) 938, 1043, 1109
AfiI CCNNNNNNNGG 3 cut(s) 384, 1479, 1516
AflII CTTAAG 1 cut(s) 41
AgeI ACCGGT 1 cut(s) 1452
AjnI CCWGG 4 cut(s) 288, 318, 333, 802
Alw21I GWGCWC 3 cut(s) 138, 830, 1000
Alw26I GTCTC 5 cut(s) 368, 542, 624, 1394, 1531
AlwI GGATC 4 cut(s) 280, 293, 330, 1287
Ama87I CYCGRG 3 cut(s) 1192, 1436, 1472
AoxI GGCC 6 cut(s) 34, 204, 291, 784, 1251, 1507
ApeKI GCWGC 4 cut(s) 672, 759, 1023, 1145
ApoI RAATTY 2 cut(s) 1373, 1581
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
AsiGI ACCGGT 1 cut(s) 1452
Asp700I GAANNNNTTC 1 cut(s) 1337
AspLEI GCGC 3 cut(s) 481, 626, 693
AspS9I GGNCC 3 cut(s) 249, 613, 1519
AsuHPI GGTGA 7 cut(s) 103, 109, 868, 1165, 1208, 1216, 1357
AsuII TTCGAA 2 cut(s) 1065, 1487
AvaI CYCGRG 3 cut(s) 1192, 1436, 1472
AvaII GGWCC 3 cut(s) 249, 613, 1519
BaeGI GKGCMC 1 cut(s) 390
BalI TGGCCA 1 cut(s) 1509
BamHI GGATCC 1 cut(s) 285
BanII GRGCYC 2 cut(s) 138, 1000
BauI CACGAG 1 cut(s) 680
BbrPI CACGTG 1 cut(s) 619
BbsI GAAGAC 1 cut(s) 1325
Bbv12I GWGCWC 3 cut(s) 138, 830, 1000
BbvI GCAGC 4 cut(s) 684, 771, 1035, 1157
BccI CCATC 5 cut(s) 215, 241, 730, 841, 1348
BceAI ACGGC 6 cut(s) 212, 278, 296, 718, 874, 883
BcgI CGANNNNNNTGC 4 cut(s) 587, 621, 661, 695
BciT130I CCWGG 4 cut(s) 290, 320, 335, 804
BclI TGATCA 1 cut(s) 1102
BcoDI GTCTC 5 cut(s) 368, 542, 624, 1394, 1531
BfrI CTTAAG 1 cut(s) 41
BfuAI ACCTGC 1 cut(s) 948
Bme18I GGWCC 3 cut(s) 249, 613, 1519
BmeT110I CYCGRG 3 cut(s) 1192, 1436, 1472
BmgT120I GGNCC 3 cut(s) 249, 613, 1519
BmiI GGNNCC 6 cut(s) 6, 13, 287, 834, 870, 1012
BmsI GCATC 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 1325
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpmI CTGGAG 1 cut(s) 997
Bpu10I CCTNAGC 1 cut(s) 837
Bpu14I TTCGAA 2 cut(s) 1065, 1487
BsaAI YACGTR 2 cut(s) 619, 985
BsaHI GRCGYC 4 cut(s) 225, 393, 417, 535
BsaI GGTCTC 2 cut(s) 368, 1394
BsaWI WCCGGW 1 cut(s) 1452
Bsc4I CCNNNNNNNGG 3 cut(s) 384, 1479, 1516
Bse118I RCCGGY 5 cut(s) 119, 194, 556, 1240, 1452
Bse1I ACTGG 2 cut(s) 287, 1510
Bse3DI GCAATG 1 cut(s) 1130
BseBI CCWGG 4 cut(s) 290, 320, 335, 804
BseGI GGATG 2 cut(s) 994, 1363
BseLI CCNNNNNNNGG 3 cut(s) 384, 1479, 1516
BseMI GCAATG 1 cut(s) 1130
BseMII CTCAG 3 cut(s) 395, 828, 1526
BseNI ACTGG 2 cut(s) 287, 1510
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 4 cut(s) 684, 771, 1035, 1157
Bsh1236I CGCG 5 cut(s) 428, 626, 691, 693, 732
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 6 cut(s) 36, 206, 293, 786, 1253, 1509
BshTI ACCGGT 1 cut(s) 1452
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 3 cut(s) 138, 830, 1000
BsiHKCI CYCGRG 3 cut(s) 1192, 1436, 1472
BslFI GGGAC 3 cut(s) 316, 441, 1381
BslI CCNNNNNNNGG 3 cut(s) 384, 1479, 1516
BsmAI GTCTC 5 cut(s) 368, 542, 624, 1394, 1531
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 3 cut(s) 316, 441, 1381
BsnI GGCC 6 cut(s) 36, 206, 293, 786, 1253, 1509
Bso31I GGTCTC 2 cut(s) 368, 1394
BsoBI CYCGRG 3 cut(s) 1192, 1436, 1472
Bsp119I TTCGAA 2 cut(s) 1065, 1487
Bsp1286I GDGCHC 4 cut(s) 138, 390, 830, 1000
Bsp143I GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
Bsp68I TCGCGA 1 cut(s) 732
BspANI GGCC 6 cut(s) 36, 206, 293, 786, 1253, 1509
BspCNI CTCAG 3 cut(s) 396, 829, 1527
BspFNI CGCG 5 cut(s) 428, 626, 691, 693, 732
BspLI GGNNCC 6 cut(s) 6, 13, 287, 834, 870, 1012
BspMI ACCTGC 1 cut(s) 948
BspPI GGATC 4 cut(s) 280, 293, 330, 1287
BspT104I TTCGAA 2 cut(s) 1065, 1487
BspTI CTTAAG 1 cut(s) 41
BspTNI GGTCTC 2 cut(s) 368, 1394
BsrBI CCGCTC 1 cut(s) 1480
BsrDI GCAATG 1 cut(s) 1130
BsrFI RCCGGY 5 cut(s) 119, 194, 556, 1240, 1452
BsrI ACTGG 2 cut(s) 287, 1510
BssAI RCCGGY 5 cut(s) 119, 194, 556, 1240, 1452
BssMI GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
BssNI GRCGYC 4 cut(s) 225, 393, 417, 535
BssSI CACGAG 1 cut(s) 680
BssT1I CCWWGG 1 cut(s) 459
Bst2BI CACGAG 1 cut(s) 680
Bst2UI CCWGG 4 cut(s) 290, 320, 335, 804
Bst4CI ACNGT 4 cut(s) 60, 115, 485, 492
Bst6I CTCTTC 1 cut(s) 1493
BstACI GRCGYC 4 cut(s) 225, 393, 417, 535
BstAFI CTTAAG 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 147
BstBAI YACGTR 2 cut(s) 619, 985
BstBI TTCGAA 2 cut(s) 1065, 1487
BstC8I GCNNGC 5 cut(s) 25, 29, 558, 677, 1242
BstDEI CTNAG 4 cut(s) 404, 822, 837, 1535
BstDSI CCRYGG 1 cut(s) 899
BstF5I GGATG 2 cut(s) 994, 1363
BstFNI CGCG 5 cut(s) 428, 626, 691, 693, 732
BstHHI GCGC 3 cut(s) 481, 626, 693
BstKTI GATC 7 cut(s) 265, 288, 325, 1105, 1182, 1282, 1422
BstMAI GTCTC 5 cut(s) 368, 542, 624, 1394, 1531
BstMBI GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
BstMCI CGRYCG 1 cut(s) 432
BstNI CCWGG 4 cut(s) 290, 320, 335, 804
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 5 cut(s) 428, 626, 691, 693, 732
BstV1I GCAGC 4 cut(s) 684, 771, 1035, 1157
BstV2I GAAGAC 1 cut(s) 1325
BstX2I RGATCY 2 cut(s) 285, 322
BstYI RGATCY 2 cut(s) 285, 322
BsuRI GGCC 6 cut(s) 36, 206, 293, 786, 1253, 1509
BtgI CCRYGG 1 cut(s) 899
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 2 cut(s) 994, 1363
BtsI GCAGTG 2 cut(s) 205, 817
BtsIMutI CAGTG 4 cut(s) 205, 817, 1517, 1532
BtuMI TCGCGA 1 cut(s) 732
BveI ACCTGC 1 cut(s) 948
Cac8I GCNNGC 5 cut(s) 25, 29, 558, 677, 1242
CfoI GCGC 3 cut(s) 481, 626, 693
Cfr10I RCCGGY 5 cut(s) 119, 194, 556, 1240, 1452
Cfr13I GGNCC 3 cut(s) 249, 613, 1519
Cfr9I CCCGGG 2 cut(s) 1192, 1472
CseI GACGC 6 cut(s) 233, 358, 406, 434, 443, 595
Csp6I GTAC 3 cut(s) 937, 1042, 1108
CspAI ACCGGT 1 cut(s) 1452
CviAII CATG 2 cut(s) 328, 1100
CviQI GTAC 3 cut(s) 937, 1042, 1108
DdeI CTNAG 4 cut(s) 404, 822, 837, 1535
DpnI GATC 7 cut(s) 264, 287, 324, 1104, 1181, 1281, 1421
DpnII GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
EaeI YGGCCR 3 cut(s) 204, 1251, 1507
Eam1104I CTCTTC 1 cut(s) 1493
EarI CTCTTC 1 cut(s) 1493
EciI GGCGGA 7 cut(s) 4, 298, 546, 575, 881, 968, 1275
Ecl136II GAGCTC 2 cut(s) 136, 998
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 2 cut(s) 138, 1000
Eco31I GGTCTC 2 cut(s) 368, 1394
Eco47I GGWCC 3 cut(s) 249, 613, 1519
Eco53kI GAGCTC 2 cut(s) 136, 998
Eco57I CTGAAG 2 cut(s) 611, 932
Eco72I CACGTG 1 cut(s) 619
Eco88I CYCGRG 3 cut(s) 1192, 1436, 1472
EcoICRI GAGCTC 2 cut(s) 136, 998
EcoO109I RGGNCCY 1 cut(s) 249
EcoRI GAATTC 1 cut(s) 1373
EcoRII CCWGG 4 cut(s) 288, 318, 333, 802
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 2 cut(s) 138, 1000
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 2 cut(s) 331, 1103
FalI AAGNNNNNCTT 2 cut(s) 1307, 1339
FaqI GGGAC 3 cut(s) 316, 441, 1381
FatI CATG 2 cut(s) 327, 1099
FbaI TGATCA 1 cut(s) 1102
FblI GTMKAC 1 cut(s) 396
FokI GGATG 2 cut(s) 1001, 1370
FriOI GRGCYC 2 cut(s) 138, 1000
GlaI GCGC 3 cut(s) 480, 625, 692
GsuI CTGGAG 1 cut(s) 997
HaeIII GGCC 6 cut(s) 36, 206, 293, 786, 1253, 1509
HgaI GACGC 6 cut(s) 233, 358, 406, 434, 443, 595
HhaI GCGC 3 cut(s) 481, 626, 693
Hin1I GRCGYC 4 cut(s) 225, 393, 417, 535
Hin1II CATG 2 cut(s) 331, 1103
Hin6I GCGC 3 cut(s) 479, 624, 691
HinP1I GCGC 3 cut(s) 479, 624, 691
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 6 cut(s) 125, 410, 792, 1234, 1285, 1384
HphI GGTGA 7 cut(s) 103, 109, 868, 1165, 1208, 1216, 1357
Hpy166II GTNNAC 3 cut(s) 397, 488, 616
Hpy188I TCNGA 6 cut(s) 912, 995, 1002, 1284, 1330, 1389
Hpy8I GTNNAC 3 cut(s) 397, 488, 616
Hpy99I CGWCG 7 cut(s) 110, 227, 374, 395, 704, 718, 1427
HpyAV CCTTC 6 cut(s) 593, 742, 1114, 1195, 1327, 1358
HpyCH4III ACNGT 4 cut(s) 60, 115, 485, 492
HpyCH4IV ACGT 8 cut(s) 48, 105, 393, 535, 618, 930, 984, 1166
HpyCH4V TGCA 2 cut(s) 672, 1145
HpyF3I CTNAG 4 cut(s) 404, 822, 837, 1535
HpySE526I ACGT 8 cut(s) 48, 105, 393, 535, 618, 930, 984, 1166
Hsp92I GRCGYC 4 cut(s) 225, 393, 417, 535
Hsp92II CATG 2 cut(s) 331, 1103
HspAI GCGC 3 cut(s) 479, 624, 691
KroI GCCGGC 2 cut(s) 556, 1240
KroNI GCCGGC 2 cut(s) 558, 1242
Ksp22I TGATCA 1 cut(s) 1102
Kzo9I GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
LmnI GCTCC 7 cut(s) 17, 850, 868, 995, 1002, 1016, 1477
Lsp1109I GCAGC 4 cut(s) 684, 771, 1035, 1157
LweI GCATC 1 cut(s) 708
MaeII ACGT 8 cut(s) 48, 105, 393, 535, 618, 930, 984, 1166
MaeIII GTNAC 5 cut(s) 109, 115, 682, 1126, 1204
MalI GATC 7 cut(s) 264, 287, 324, 1104, 1181, 1281, 1421
MbiI CCGCTC 1 cut(s) 1480
MboI GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
MboII GAAGA 9 cut(s) 145, 295, 925, 928, 1129, 1327, 1330, 1363, 1510
MfeI CAATTG 1 cut(s) 667
MflI RGATCY 2 cut(s) 285, 322
MhlI GDGCHC 4 cut(s) 138, 390, 830, 1000
MlsI TGGCCA 1 cut(s) 1509
MluCI AATT 7 cut(s) 465, 659, 667, 709, 1306, 1373, 1581
MluNI TGGCCA 1 cut(s) 1509
MlyI GAGTC 3 cut(s) 134, 419, 801
MmeI TCCRAC 1 cut(s) 973
Mox20I TGGCCA 1 cut(s) 1509
MroNI GCCGGC 2 cut(s) 556, 1240
MroXI GAANNNNTTC 1 cut(s) 1337
MscI TGGCCA 1 cut(s) 1509
MseI TTAA 5 cut(s) 42, 174, 441, 933, 1266
Msp20I TGGCCA 1 cut(s) 1509
MspA1I CMGCKG 2 cut(s) 762, 1026
MspCI CTTAAG 1 cut(s) 41
MunI CAATTG 1 cut(s) 667
MvaI CCWGG 4 cut(s) 290, 320, 335, 804
MvnI CGCG 5 cut(s) 428, 626, 691, 693, 732
NaeI GCCGGC 2 cut(s) 558, 1242
NdeII GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
NgoMIV GCCGGC 2 cut(s) 556, 1240
NlaIII CATG 2 cut(s) 331, 1103
NlaIV GGNNCC 6 cut(s) 6, 13, 287, 834, 870, 1012
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 5 cut(s) 109, 115, 682, 1126, 1204
NruI TCGCGA 1 cut(s) 732
NspV TTCGAA 2 cut(s) 1065, 1487
PaeR7I CTCGAG 1 cut(s) 1436
PasI CCCWGGG 1 cut(s) 334
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 111, 242
PdiI GCCGGC 2 cut(s) 558, 1242
PdmI GAANNNNTTC 1 cut(s) 1337
PfeI GAWTC 3 cut(s) 1234, 1285, 1384
PflFI GACNNNGTC 1 cut(s) 428
PflMI CCANNNNNTGG 1 cut(s) 1516
PfoI TCCNGGA 3 cut(s) 162, 318, 347
PinAI ACCGGT 1 cut(s) 1452
PleI GAGTC 3 cut(s) 133, 418, 800
PmaCI CACGTG 1 cut(s) 619
PmlI CACGTG 1 cut(s) 619
PpsI GAGTC 3 cut(s) 133, 418, 800
Ppu21I YACGTR 2 cut(s) 619, 985
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 2 cut(s) 138, 1000
Psp1406I AACGTT 3 cut(s) 48, 930, 1166
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 4 cut(s) 288, 318, 333, 802
PspCI CACGTG 1 cut(s) 619
PspGI CCWGG 4 cut(s) 288, 318, 333, 802
PspN4I GGNNCC 6 cut(s) 6, 13, 287, 834, 870, 1012
PspPI GGNCC 3 cut(s) 249, 613, 1519
PspPPI RGGWCCY 1 cut(s) 249
PsrI GAACNNNNNNTAC 2 cut(s) 920, 952
PsuI RGATCY 2 cut(s) 285, 322
PsyI GACNNNGTC 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 1026
RruI TCGCGA 1 cut(s) 732
RsaI GTAC 3 cut(s) 938, 1043, 1109
RsaNI GTAC 3 cut(s) 937, 1042, 1108
SacI GAGCTC 2 cut(s) 138, 1000
SaqAI TTAA 5 cut(s) 42, 174, 441, 933, 1266
Sau3AI GATC 7 cut(s) 262, 285, 322, 1102, 1179, 1279, 1419
Sau96I GGNCC 3 cut(s) 249, 613, 1519
SchI GAGTC 3 cut(s) 134, 419, 801
SduI GDGCHC 4 cut(s) 138, 390, 830, 1000
SfaNI GCATC 1 cut(s) 708
Sfr274I CTCGAG 1 cut(s) 1436
SfuI TTCGAA 2 cut(s) 1065, 1487
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 3 cut(s) 249, 613, 1519
SlaI CTCGAG 1 cut(s) 1436
SmaI CCCGGG 2 cut(s) 1194, 1474
SmlI CTYRAG 2 cut(s) 41, 1436
SmoI CTYRAG 2 cut(s) 41, 1436
Sse9I AATT 7 cut(s) 465, 659, 667, 709, 1306, 1373, 1581
SstI GAGCTC 2 cut(s) 138, 1000
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 4 cut(s) 60, 115, 485, 492
TaiI ACGT 8 cut(s) 51, 108, 396, 538, 621, 933, 987, 1169
TasI AATT 7 cut(s) 465, 659, 667, 709, 1306, 1373, 1581
TatI WGTACW 1 cut(s) 1107
TfiI GAWTC 3 cut(s) 1234, 1285, 1384
Tru1I TTAA 5 cut(s) 42, 174, 441, 933, 1266
Tru9I TTAA 5 cut(s) 42, 174, 441, 933, 1266
TscAI CASTG 4 cut(s) 205, 817, 1517, 1539
TseFI GTSAC 5 cut(s) 109, 115, 682, 1126, 1204
TseI GCWGC 4 cut(s) 672, 759, 1023, 1145
Tsp45I GTSAC 5 cut(s) 109, 115, 682, 1126, 1204
TspDTI ATGAA 3 cut(s) 1176, 1364, 1547
TspGWI ACGGA 2 cut(s) 1223, 1385
TspMI CCCGGG 2 cut(s) 1192, 1472
TspRI CASTG 4 cut(s) 205, 817, 1517, 1539
Tth111I GACNNNGTC 1 cut(s) 428
Van91I CCANNNNNTGG 1 cut(s) 1516
Vha464I CTTAAG 1 cut(s) 41
VpaK11BI GGWCC 3 cut(s) 249, 613, 1519
XapI RAATTY 2 cut(s) 1373, 1581
XhoI CTCGAG 1 cut(s) 1436
XmaI CCCGGG 2 cut(s) 1192, 1472
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 1337
ZraI GACGTC 2 cut(s) 394, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.