MD12G1180800.v1.1

Methyltransferase FkbM domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013085
Physical Location & Seq
Reverse (-)
26069138 .. 26070700
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1180800.v1.1.491

Sequence Viewer

Length: 1563 bp
ATGGAACCCGGCGCCGCCGCGGCAGGCAAGCCAGGCCTACTGAAGAACGTTTTTATACGGTTCTTTTTGTTCGGCGTTTTGATCGTGCTCTGTCGCTTCGCCTACGTCGTCACCGTCACCGGCGAGTCGTGCGAGCTCGGGAACTTCTGCTTCTTCTCTCTGCCGGAAAATCTCAACTTCGTTATAGCGAATACTGGCGGTTCGGCCATCGCCGCAAAAAAAGAAGCCGTTCCGTCGATTTCGGTGGGGTCCACGAAGCCCGAACTCTACACCAGCAAGGACTGGATCAAGGCTGTCCATTTCTACTCCGCCGTGTTCCAGGATCTCATGTCTCAGGGCTTCCTCTCCCGGAAAGCCAAGTCGCTCTGCGTCGAGACCCCCGCCGGGCACGACGTCTACGCCCTGAGAGAGTCCGGCGTCAAGGGCGCGGTCGGTGTTTTCAAGAAAGCGTCGAGACCTTTGGTAATTCCCGGCGAATCGCACCGTTTGCCGTTCGCTGACAACAGCTTTGACTTTGTTTTCTCCGGCGGAGGACGGCTCGAGAAGTCGCCAAAGCCGGCGGATGTCGCCGCCGAGATCGTGCGGACGCTGAAGCCCGAAGGGTTTGCGGTGGTCCACGTCGGCGCGAGAGACACTTACAGCTTCCATTCGTTTATTGAATTGTTCAATTGCAGCAAGCTCGTGACTTCGCGGGACATCGACGGCTTCGATCCGCCGATGCCGAAGATTCGCGAGATGGTTTTGAGGAAGGATTGCGGCGAGAGTGAGGGTTTCGGTCACAGGGCCGAGAACCCTGGTGGCGGTGCTGGTAATAAGTGCTCAGTTCCTGGGCATAAGATGGATCTCATCAGAAAGGCCGAGCCTTTGATTGAAATAGAGCCGTTGAAGCCGTGGATTACTCTGAAGAAGAACATACAGAACGTGAAGTACCTCCCGACAATGGCGGATATAAGCTTCAAGAAGCGGTATGCTTACGTGGATGTCGGAGCTCGGAGCTATGGCTCCAGCATTGGCAGCTGGTTCAAGAAGCAGTACCCGAAACAGAACAGGACTTTCGAAGTTTTCGCCATTGAAGCCGATAAGACTTTTCATGACCAGTACAAGTTGAAGAAGGGGGTCACATTGCTGCCTTACGCAGCTTGGGTGAGGAACGAGACATTGTCCTTCGAGATCAATGGCGACCCGGGTGAGAAGGTGAAGGATAAAGGGAGAGGAATGGGGAGGATTCAGCCGGCGAATCCAGCCAGTGGGGGGTTTAACGGAGAGGTGGATCGGATTCAGGGGTTTGATTTTGTGAATTGGTTGAAGAACACCTTTTCGGAAAAGGATTTTGTGGTGATGAAGATGGATGTGGAAGGGACGGAATTCGATTTGATTCCGAGGCTTTTCGAGACAGGGGCAATCTGTTTGATCGACGAGGTTTTTCTCGAGTGCCATTACAATCGGTGGCAGAGGTGTTGCCCCGGCGAGAGGAGTTCCAAGTATGAGAAGAGTTACGGTCAGTGTTTGGACCTGTATGCTTCACTTAGACAAAGTGGAGTTCTTGTTCATCAATGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_21

Protein Analysis

521

Amino Acids

57.95

Weight (kDa)

8.8

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 155 - 204 2.1e-07 Methyltransferase domain
DUF7870 PF25276 296 - 371 6.6e-09 Domain of unknown function (DUF7870)
DUF7870 PF25276 427 - 520 3.9e-20 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013085)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01710
fragaria_vesca FvH4_6g10540
malus_domestica MD04G1167500.v1.1 MD12G1180800.v1.1
prunus_persica Prupe.6G285700_v2.0.a1
pyrus_communis pycom04g14860 pycom12g17040
rosa_chinensis RchiOBHm_Chr3g0461501
rosa_laevigata RLG00000024927
rosa_multiflora Rmu_sc0002677.1_g000002
rosa_roxburghii Rroxscaffold_6G00418230 Rroxscaffold_6G00418560
rosa_rugosa Rorug03G0051300
rosa_samantha Rh3AG109100 Rh3BG112900 Rh3CG114400 Rh3DG114500
rosa_wichuraiana Rw3G009270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 396
AccB1I GGYRCC 1 cut(s) 11
AccB7I CCANNNNNTGG 1 cut(s) 1247
AccI GTMKAC 1 cut(s) 396
AccII CGCG 5 cut(s) 20, 428, 626, 691, 732
AclI AACGTT 1 cut(s) 48
AclWI GGATC 5 cut(s) 293, 330, 704, 849, 1278
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 1364
AcuI CTGAAG 3 cut(s) 62, 611, 923
AcyI GRCGYC 3 cut(s) 12, 393, 417
AfaI GTAC 3 cut(s) 929, 1034, 1100
AfiI CCNNNNNNNGG 6 cut(s) 384, 800, 940, 1247, 1251, 1470
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 4 cut(s) 31, 318, 793, 826
AloI GAACNNNNNNTCC 2 cut(s) 1530, 1562
AluBI AGCT 9 cut(s) 136, 507, 642, 679, 954, 989, 996, 1017, 1139
AluI AGCT 9 cut(s) 136, 507, 642, 679, 954, 989, 996, 1017, 1139
Alw21I GWGCWC 4 cut(s) 90, 138, 821, 991
Alw26I GTCTC 6 cut(s) 336, 368, 448, 624, 1148, 1385
AlwI GGATC 5 cut(s) 293, 330, 704, 849, 1278
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 4 cut(s) 137, 539, 1183, 1427
AoxI GGCC 4 cut(s) 34, 204, 783, 855
ApeKI GCWGC 4 cut(s) 672, 1014, 1126, 1136
ApoI RAATTY 1 cut(s) 1364
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 3 cut(s) 14, 428, 626
AspS9I GGNCC 4 cut(s) 249, 613, 783, 1510
AsuC2I CCSGG 7 cut(s) 9, 349, 385, 471, 1184, 1185, 1464
AsuHPI GGTGA 6 cut(s) 103, 109, 1156, 1199, 1207, 1348
AsuII TTCGAA 1 cut(s) 1056
AvaI CYCGRG 4 cut(s) 137, 539, 1183, 1427
AvaII GGWCC 3 cut(s) 249, 613, 1510
BaeGI GKGCMC 1 cut(s) 390
BanI GGYRCC 1 cut(s) 11
BanII GRGCYC 2 cut(s) 138, 991
BauI CACGAG 1 cut(s) 680
Bbv12I GWGCWC 4 cut(s) 90, 138, 821, 991
BbvI GCAGC 4 cut(s) 684, 1026, 1113, 1148
BccI CCATC 4 cut(s) 215, 730, 832, 1339
BceAI ACGGC 7 cut(s) 212, 296, 475, 551, 718, 865, 874
BcgI CGANNNNNNTGC 4 cut(s) 587, 621, 661, 695
BciT130I CCWGG 4 cut(s) 33, 320, 795, 828
BcnI CCSGG 7 cut(s) 9, 349, 385, 471, 1184, 1185, 1464
BcoDI GTCTC 6 cut(s) 336, 368, 448, 624, 1148, 1385
BfoI RGCGCY 1 cut(s) 15
BglI GCCNNNNNGGC 1 cut(s) 20
Bme18I GGWCC 3 cut(s) 249, 613, 1510
BmeT110I CYCGRG 4 cut(s) 137, 539, 1183, 1427
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 4 cut(s) 249, 613, 783, 1510
BmiI GGNNCC 4 cut(s) 6, 13, 250, 1003
BmsI GCATC 1 cut(s) 708
BplI GAGNNNNNCTC 2 cut(s) 522, 554
BpmI CTGGAG 1 cut(s) 988
Bpu14I TTCGAA 1 cut(s) 1056
BpuMI CCSGG 7 cut(s) 9, 349, 385, 471, 1184, 1185, 1464
BsaAI YACGTR 1 cut(s) 976
BsaHI GRCGYC 3 cut(s) 12, 393, 417
BsaI GGTCTC 2 cut(s) 368, 448
BsaJI CCNNGG 7 cut(s) 18, 793, 827, 890, 1183, 1379, 1462
BsaXI ACNNNNNCTCC 2 cut(s) 1530, 1560
Bsc4I CCNNNNNNNGG 6 cut(s) 384, 800, 940, 1247, 1251, 1470
Bse118I RCCGGY 3 cut(s) 119, 556, 1231
Bse1I ACTGG 4 cut(s) 199, 287, 1096, 1245
Bse3DI GCAATG 1 cut(s) 1121
BseBI CCWGG 4 cut(s) 33, 320, 795, 828
BseDI CCNNGG 7 cut(s) 18, 793, 827, 890, 1183, 1379, 1462
BseGI GGATG 3 cut(s) 568, 985, 1354
BseLI CCNNNNNNNGG 6 cut(s) 384, 800, 940, 1247, 1251, 1470
BseMI GCAATG 1 cut(s) 1121
BseMII CTCAG 3 cut(s) 347, 395, 834
BseNI ACTGG 4 cut(s) 199, 287, 1096, 1245
BseRI GAGGAG 1 cut(s) 1486
BseSI GKGCMC 1 cut(s) 390
BseXI GCAGC 4 cut(s) 684, 1026, 1113, 1148
Bsh1236I CGCG 5 cut(s) 20, 428, 626, 691, 732
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 36, 206, 785, 857
BshNI GGYRCC 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 4 cut(s) 90, 138, 821, 991
BsiHKCI CYCGRG 4 cut(s) 137, 539, 1183, 1427
BslFI GGGAC 2 cut(s) 707, 1372
BslI CCNNNNNNNGG 6 cut(s) 384, 800, 940, 1247, 1251, 1470
BsmAI GTCTC 6 cut(s) 336, 368, 448, 624, 1148, 1385
BsmFI GGGAC 2 cut(s) 707, 1372
BsnI GGCC 4 cut(s) 36, 206, 785, 857
Bso31I GGTCTC 2 cut(s) 368, 448
BsoBI CYCGRG 4 cut(s) 137, 539, 1183, 1427
Bsp119I TTCGAA 1 cut(s) 1056
Bsp1286I GDGCHC 5 cut(s) 90, 138, 390, 821, 991
Bsp143I GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
Bsp68I TCGCGA 1 cut(s) 732
BspANI GGCC 4 cut(s) 36, 206, 785, 857
BspCNI CTCAG 3 cut(s) 346, 396, 833
BspFNI CGCG 5 cut(s) 20, 428, 626, 691, 732
BspHI TCATGA 1 cut(s) 1090
BspLI GGNNCC 4 cut(s) 6, 13, 250, 1003
BspPI GGATC 5 cut(s) 293, 330, 704, 849, 1278
BspT104I TTCGAA 1 cut(s) 1056
BspT107I GGYRCC 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 368, 448
BsrDI GCAATG 1 cut(s) 1121
BsrFI RCCGGY 3 cut(s) 119, 556, 1231
BsrI ACTGG 4 cut(s) 199, 287, 1096, 1245
BssAI RCCGGY 3 cut(s) 119, 556, 1231
BssECI CCNNGG 7 cut(s) 18, 793, 827, 890, 1183, 1379, 1462
BssMI GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
BssNI GRCGYC 3 cut(s) 12, 393, 417
BssSI CACGAG 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 680
Bst2UI CCWGG 4 cut(s) 33, 320, 795, 828
Bst4CI ACNGT 4 cut(s) 60, 115, 485, 1499
Bst6I CTCTTC 1 cut(s) 1484
BstACI GRCGYC 3 cut(s) 12, 393, 417
BstAPI GCANNNNNTGC 1 cut(s) 487
BstBAI YACGTR 1 cut(s) 976
BstBI TTCGAA 1 cut(s) 1056
BstC8I GCNNGC 6 cut(s) 25, 29, 134, 558, 677, 1233
BstDEI CTNAG 4 cut(s) 333, 404, 820, 1526
BstDSI CCRYGG 2 cut(s) 18, 890
BstF5I GGATG 3 cut(s) 568, 985, 1354
BstFNI CGCG 5 cut(s) 20, 428, 626, 691, 732
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 3 cut(s) 14, 428, 626
BstKTI GATC 9 cut(s) 84, 288, 325, 579, 712, 844, 1173, 1273, 1413
BstMAI GTCTC 6 cut(s) 336, 368, 448, 624, 1148, 1385
BstMBI GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
BstMCI CGRYCG 1 cut(s) 432
BstNI CCWGG 4 cut(s) 33, 320, 795, 828
BstSLI GKGCMC 1 cut(s) 390
BstUI CGCG 5 cut(s) 20, 428, 626, 691, 732
BstV1I GCAGC 4 cut(s) 684, 1026, 1113, 1148
BstX2I RGATCY 2 cut(s) 322, 841
BstYI RGATCY 2 cut(s) 322, 841
BsuRI GGCC 4 cut(s) 36, 206, 785, 857
BtgI CCRYGG 2 cut(s) 18, 890
BtgZI GCGATG 1 cut(s) 193
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 3 cut(s) 568, 985, 1354
BtsIMutI CAGTG 2 cut(s) 1252, 1508
BtuMI TCGCGA 1 cut(s) 732
Cac8I GCNNGC 6 cut(s) 25, 29, 134, 558, 677, 1233
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 1090
CfoI GCGC 3 cut(s) 14, 428, 626
Cfr10I RCCGGY 3 cut(s) 119, 556, 1231
Cfr13I GGNCC 4 cut(s) 249, 613, 783, 1510
Cfr42I CCGCGG 1 cut(s) 21
Cfr9I CCCGGG 1 cut(s) 1183
CseI GACGC 4 cut(s) 358, 406, 438, 595
Csp6I GTAC 3 cut(s) 928, 1033, 1099
CviAII CATG 2 cut(s) 328, 1091
CviQI GTAC 3 cut(s) 928, 1033, 1099
DdeI CTNAG 4 cut(s) 333, 404, 820, 1526
DinI GGCGCC 1 cut(s) 13
DpnI GATC 9 cut(s) 83, 287, 324, 578, 711, 843, 1172, 1272, 1412
DpnII GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 1484
EarI CTCTTC 1 cut(s) 1484
EciI GGCGGA 5 cut(s) 298, 543, 575, 702, 959
Ecl136II GAGCTC 2 cut(s) 136, 989
Eco147I AGGCCT 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 138, 991
Eco31I GGTCTC 2 cut(s) 368, 448
Eco47I GGWCC 3 cut(s) 249, 613, 1510
Eco53kI GAGCTC 2 cut(s) 136, 989
Eco57I CTGAAG 3 cut(s) 62, 611, 923
Eco88I CYCGRG 4 cut(s) 137, 539, 1183, 1427
EcoICRI GAGCTC 2 cut(s) 136, 989
EcoRI GAATTC 1 cut(s) 1364
EcoRII CCWGG 4 cut(s) 31, 318, 793, 826
EcoT38I GRGCYC 2 cut(s) 138, 991
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
FaeI CATG 2 cut(s) 331, 1094
FalI AAGNNNNNCTT 2 cut(s) 1298, 1330
FaqI GGGAC 2 cut(s) 707, 1372
FatI CATG 2 cut(s) 327, 1090
FauI CCCGC 2 cut(s) 388, 684
FblI GTMKAC 1 cut(s) 396
FokI GGATG 3 cut(s) 575, 992, 1361
FriOI GRGCYC 2 cut(s) 138, 991
GlaI GCGC 3 cut(s) 13, 427, 625
GsuI CTGGAG 1 cut(s) 988
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 4 cut(s) 36, 206, 785, 857
HgaI GACGC 4 cut(s) 358, 406, 438, 595
HhaI GCGC 3 cut(s) 14, 428, 626
Hin1I GRCGYC 3 cut(s) 12, 393, 417
Hin1II CATG 2 cut(s) 331, 1094
Hin6I GCGC 3 cut(s) 12, 426, 624
HinP1I GCGC 3 cut(s) 12, 426, 624
HindIII AAGCTT 1 cut(s) 952
HinfI GANTC 8 cut(s) 125, 410, 476, 727, 1225, 1237, 1276, 1375
HphI GGTGA 6 cut(s) 103, 109, 1156, 1199, 1207, 1348
Hpy166II GTNNAC 3 cut(s) 252, 397, 616
Hpy188I TCNGA 7 cut(s) 851, 903, 986, 993, 1275, 1321, 1380
Hpy8I GTNNAC 3 cut(s) 252, 397, 616
Hpy99I CGWCG 8 cut(s) 110, 238, 374, 395, 454, 623, 704, 1418
HpyAV CCTTC 7 cut(s) 593, 742, 1105, 1174, 1186, 1192, 1349
HpyCH4III ACNGT 4 cut(s) 60, 115, 485, 1499
HpyCH4IV ACGT 6 cut(s) 48, 105, 393, 618, 921, 975
HpyCH4V TGCA 1 cut(s) 672
HpyF3I CTNAG 4 cut(s) 333, 404, 820, 1526
HpySE526I ACGT 6 cut(s) 48, 105, 393, 618, 921, 975
Hsp92I GRCGYC 3 cut(s) 12, 393, 417
Hsp92II CATG 2 cut(s) 331, 1094
HspAI GCGC 3 cut(s) 12, 426, 624
KasI GGCGCC 1 cut(s) 11
KroI GCCGGC 2 cut(s) 556, 1231
KroNI GCCGGC 2 cut(s) 558, 1233
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
LmnI GCTCC 3 cut(s) 986, 993, 1007
Lsp1109I GCAGC 4 cut(s) 684, 1026, 1113, 1148
LweI GCATC 1 cut(s) 708
MaeII ACGT 6 cut(s) 48, 105, 393, 618, 921, 975
MaeIII GTNAC 6 cut(s) 109, 115, 682, 776, 1117, 1493
MalI GATC 9 cut(s) 83, 287, 324, 578, 711, 843, 1172, 1272, 1412
MboI GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
MboII GAAGA 9 cut(s) 55, 145, 736, 916, 919, 1120, 1318, 1354, 1501
MfeI CAATTG 1 cut(s) 667
MflI RGATCY 2 cut(s) 322, 841
MhlI GDGCHC 5 cut(s) 90, 138, 390, 821, 991
MluCI AATT 5 cut(s) 465, 659, 667, 1297, 1364
Mly113I GGCGCC 1 cut(s) 12
MlyI GAGTC 2 cut(s) 134, 419
MmeI TCCRAC 1 cut(s) 964
MroNI GCCGGC 2 cut(s) 556, 1231
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 1 cut(s) 1257
MspA1I CMGCKG 2 cut(s) 20, 1017
MunI CAATTG 1 cut(s) 667
MvaI CCWGG 4 cut(s) 33, 320, 795, 828
MvnI CGCG 5 cut(s) 20, 428, 626, 691, 732
NaeI GCCGGC 2 cut(s) 558, 1233
NarI GGCGCC 1 cut(s) 12
NciI CCSGG 7 cut(s) 9, 349, 385, 471, 1184, 1185, 1464
NdeII GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
NgoMIV GCCGGC 2 cut(s) 556, 1231
NlaIII CATG 2 cut(s) 331, 1094
NlaIV GGNNCC 4 cut(s) 6, 13, 250, 1003
NmeAIII GCCGAG 3 cut(s) 598, 811, 883
NmuCI GTSAC 5 cut(s) 109, 115, 682, 776, 1117
NruI TCGCGA 1 cut(s) 732
NspV TTCGAA 1 cut(s) 1056
PaeR7I CTCGAG 2 cut(s) 539, 1427
PagI TCATGA 1 cut(s) 1090
PceI AGGCCT 1 cut(s) 36
PcsI WCGNNNNNNNCGW 3 cut(s) 105, 111, 705
PdiI GCCGGC 2 cut(s) 558, 1233
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 6 cut(s) 476, 727, 1225, 1237, 1276, 1375
PflFI GACNNNGTC 1 cut(s) 1159
PflMI CCANNNNNTGG 1 cut(s) 1247
PfoI TCCNGGA 2 cut(s) 318, 347
PleI GAGTC 2 cut(s) 133, 418
PluTI GGCGCC 1 cut(s) 15
PpsI GAGTC 2 cut(s) 133, 418
Ppu21I YACGTR 1 cut(s) 976
Psp124BI GAGCTC 2 cut(s) 138, 991
Psp1406I AACGTT 1 cut(s) 48
Psp6I CCWGG 4 cut(s) 31, 318, 793, 826
PspGI CCWGG 4 cut(s) 31, 318, 793, 826
PspN4I GGNNCC 4 cut(s) 6, 13, 250, 1003
PspPI GGNCC 4 cut(s) 249, 613, 783, 1510
PsrI GAACNNNNNNTAC 2 cut(s) 911, 943
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 322, 841
PsyI GACNNNGTC 1 cut(s) 1159
PvuII CAGCTG 1 cut(s) 1017
RruI TCGCGA 1 cut(s) 732
RsaI GTAC 3 cut(s) 929, 1034, 1100
RsaNI GTAC 3 cut(s) 928, 1033, 1099
SacI GAGCTC 2 cut(s) 138, 991
SacII CCGCGG 1 cut(s) 21
SaqAI TTAA 1 cut(s) 1257
Sau3AI GATC 9 cut(s) 81, 285, 322, 576, 709, 841, 1170, 1270, 1410
Sau96I GGNCC 4 cut(s) 249, 613, 783, 1510
SchI GAGTC 2 cut(s) 134, 419
SduI GDGCHC 5 cut(s) 90, 138, 390, 821, 991
SfaNI GCATC 1 cut(s) 708
SfoI GGCGCC 1 cut(s) 13
Sfr274I CTCGAG 2 cut(s) 539, 1427
Sfr303I CCGCGG 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 1056
SgrAI CRCCGGYG 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 3 cut(s) 249, 613, 1510
SlaI CTCGAG 2 cut(s) 539, 1427
SmaI CCCGGG 1 cut(s) 1185
SmlI CTYRAG 2 cut(s) 539, 1427
SmoI CTYRAG 2 cut(s) 539, 1427
Sse9I AATT 5 cut(s) 465, 659, 667, 1297, 1364
SseBI AGGCCT 1 cut(s) 36
SspDI GGCGCC 1 cut(s) 11
SstI GAGCTC 2 cut(s) 138, 991
StuI AGGCCT 1 cut(s) 36
TaaI ACNGT 4 cut(s) 60, 115, 485, 1499
TaiI ACGT 6 cut(s) 51, 108, 396, 621, 924, 978
TaqII GACCGA 1 cut(s) 764
TasI AATT 5 cut(s) 465, 659, 667, 1297, 1364
TatI WGTACW 1 cut(s) 1098
TauI GCSGC 6 cut(s) 17, 20, 23, 215, 572, 759
TfiI GAWTC 6 cut(s) 476, 727, 1225, 1237, 1276, 1375
Tru1I TTAA 1 cut(s) 1257
Tru9I TTAA 1 cut(s) 1257
TscAI CASTG 2 cut(s) 1252, 1508
TseFI GTSAC 5 cut(s) 109, 115, 682, 776, 1117
TseI GCWGC 4 cut(s) 672, 1014, 1126, 1136
Tsp45I GTSAC 5 cut(s) 109, 115, 682, 776, 1117
TspDTI ATGAA 3 cut(s) 1079, 1355, 1538
TspGWI ACGGA 3 cut(s) 222, 1275, 1376
TspMI CCCGGG 1 cut(s) 1183
TspRI CASTG 2 cut(s) 1252, 1508
Tth111I GACNNNGTC 1 cut(s) 1159
Van91I CCANNNNNTGG 1 cut(s) 1247
VpaK11BI GGWCC 3 cut(s) 249, 613, 1510
XapI RAATTY 1 cut(s) 1364
XhoI CTCGAG 2 cut(s) 539, 1427
XmaI CCCGGG 1 cut(s) 1183
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 228
ZraI GACGTC 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.