AT5G03040

IQ-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008563
Physical Location & Seq
Reverse (-)
709948 .. 713133
3186 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03040.2

Sequence Viewer

Length: 1386 bp
ATGGGGAAAAAAGCTAAATGGTTTTCAAGTGTTAAGAAAGCATTCAGCCCAGATTCAAAGAAGTCGAAGCAAAAATTGGCTGAGGGACAAAATGGTGTTATCTCTAATCCTCCTGTTGTGGATAATGTTAGACAATCTTCTTCTTCTCCTCCTCCTGCTCTTGCTCCTCGTGAAGTGAGAGTAGCTGAAGTGATTGTTGAACGGAACAGGGATCTTTCACCTCCTTCTACAGCAGATGCTGTGAATGTTACAGCTACTGATGTTCCTGTAGTTCCATCTTCATCTGCTCCTGGTGTTGTTCGTCGCGCTACACCTACTCGATTTGCTGGAAAGTCAAACGAAGAAGCCGCTGCTATCTTGATCCAGACTATATTTAGAGGTTATTTGGCAAGGAGAGCGTTGCGGGCAATGAGGGGTTTGGTCAGACTTAAGTTATTGATGGAAGGATCTGTTGTTAAGCGGCAAGCTGCAAATACTCTAAAATGTATGCAGACTCTCTCTCGTGTACAGTCGCAGATCCGAGCTAGGAGAATCAGGATGTCAGAAGAGAATCAGGCTCGCCAGAAACAACTCCTTCAGAAACATGCTAAAGAGCTAGCTGGCTTGAAGAACGGGGATAACTGGAATGATAGCATTCAATCAAAGGAGAAAGTTGAAGCGAATTTGCTAAGCAAGTACGAGGCAACAATGAGAAGGGAAAGGGCATTGGCTTATTCATACTCTCATCAGCAAAACTGGAAGAACAACTCTAAATCTGGAAACCCGATGTTCATGGATCCAAGCAACCCGACATGGGGTTGGAGCTGGTTAGAGAGATGGATGGCTGGTAGGCCACTAGAGAGTTCCGAGAAAGAACAAAGCAACAGCAACAATGACAATGCTGCCTCGGTCAAGGGCTCTATTAACCGCAACGAAGCTGCAAAATCTCTAACCCGCAATGGCTCAACTCAACCAAACACACCATCATCCGCAAGAGGGACCCCAAGAAACAAAAACAGTTTCTTCTCACCTCCAACTCCCTCAAGGCTAAACCAATCCTCGAGGAAATCCAATGACGACGACTCCAAAAGCACAATCTCGGTCCTGTCCGAGAGGAACCGCAGACACAGCATTGCTGGTTCATCAGTCAGAGACGATGAGAGCCTCGCTGGCTCACCAGCTCTCCCGAGCTACATGGTTCCAACTAAATCAGCTCGAGCCAGGCTCAAGCCCCAAAGCCCATTAGGTGGTACCACACAGGAAAACGAAGGGTTCACAGACAAGGCATCAGCTAAGAAACGGCTCTCGTATCCAACTTCGCCTGCATTGCCTAAACCACGGCGGTTCTCAGCTCCCCCTAAGGTGGAGAGTGGCGGCGTTACCGTGACCAACGGAGCAGGCAGCTGA

Protein Analysis

461

Amino Acids

50.46

Weight (kDa)

10.64

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 366 - 435 8.6e-06 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1039
Acc65I GGTACC 1 cut(s) 1229
AccB1I GGYRCC 1 cut(s) 1229
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccII CGCG 1 cut(s) 306
AciI CCGC 9 cut(s) 348, 403, 460, 907, 934, 969, 1099, 1321, 1353
AclWI GGATC 6 cut(s) 219, 355, 454, 511, 770, 783
AcsI RAATTY 1 cut(s) 661
AcuI CTGAAG 2 cut(s) 207, 560
AfaI GTAC 3 cut(s) 507, 677, 1231
AfiI CCNNNNNNNGG 5 cut(s) 793, 794, 975, 1226, 1342
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 6 cut(s) 27, 57, 200, 607, 638, 656
AjnI CCWGG 2 cut(s) 289, 1199
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
Alw26I GTCTC 1 cut(s) 1125
AlwI GGATC 6 cut(s) 219, 355, 454, 511, 770, 783
AlwNI CAGNNNCTG 2 cut(s) 239, 257
Ama87I CYCGRG 3 cut(s) 1039, 1165, 1194
AoxI GGCC 1 cut(s) 830
ApeKI GCWGC 5 cut(s) 350, 467, 881, 917, 1380
ApoI RAATTY 1 cut(s) 661
Asp700I GAANNNNTTC 1 cut(s) 41
Asp718I GGTACC 1 cut(s) 1229
AspLEI GCGC 1 cut(s) 308
AspS9I GGNCC 2 cut(s) 978, 1081
AsuHPI GGTGA 3 cut(s) 210, 999, 1146
AsuNHI GCTAGC 1 cut(s) 595
AvaI CYCGRG 3 cut(s) 1039, 1165, 1194
AvaII GGWCC 2 cut(s) 978, 1081
AxyI CCTNAGG 1 cut(s) 1338
BamHI GGATCC 1 cut(s) 775
BanI GGYRCC 1 cut(s) 1229
BanII GRGCYC 1 cut(s) 899
BauI CACGAG 2 cut(s) 168, 501
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 4 cut(s) 337, 454, 868, 904
BccI CCATC 5 cut(s) 283, 433, 810, 814, 970
BceAI ACGGC 2 cut(s) 1295, 1334
BciT130I CCWGG 2 cut(s) 291, 1201
BciVI GTATCC 1 cut(s) 1299
BcoDI GTCTC 1 cut(s) 1125
BfaI CTAG 3 cut(s) 525, 596, 836
BfmI CTRYAG 2 cut(s) 228, 267
BfrI CTTAAG 1 cut(s) 428
BfuI GTATCC 1 cut(s) 1299
BglI GCCNNNNNGGC 1 cut(s) 1149
BisI GCNGC 8 cut(s) 348, 351, 461, 468, 882, 918, 1354, 1381
BlpI GCTNAGC 1 cut(s) 668
BlsI GCNGC 8 cut(s) 349, 352, 462, 469, 883, 919, 1355, 1382
Bme1390I CCNGG 2 cut(s) 291, 1201
Bme18I GGWCC 2 cut(s) 978, 1081
BmeT110I CYCGRG 3 cut(s) 1039, 1165, 1194
BmgT120I GGNCC 2 cut(s) 978, 1081
BmiI GGNNCC 6 cut(s) 777, 979, 980, 1097, 1179, 1231
BmrFI CCNGG 2 cut(s) 291, 1201
BmsI GCATC 2 cut(s) 226, 1274
BmtI GCTAGC 1 cut(s) 599
BplI GAGNNNNNCTC 2 cut(s) 1188, 1220
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 668
BpuEI CTTGAG 2 cut(s) 1006, 1190
BsaJI CCNNGG 2 cut(s) 885, 1316
BsaXI ACNNNNNCTCC 4 cut(s) 1046, 1076, 1146, 1176
Bsc4I CCNNNNNNNGG 5 cut(s) 793, 794, 975, 1226, 1342
Bse1I ACTGG 2 cut(s) 626, 740
Bse21I CCTNAGG 1 cut(s) 1338
Bse3DI GCAATG 4 cut(s) 414, 943, 1110, 1304
BseBI CCWGG 2 cut(s) 291, 1201
BseDI CCNNGG 2 cut(s) 885, 1316
BseGI GGATG 3 cut(s) 543, 825, 965
BseLI CCNNNNNNNGG 5 cut(s) 793, 794, 975, 1226, 1342
BseMI GCAATG 4 cut(s) 414, 943, 1110, 1304
BseMII CTCAG 2 cut(s) 72, 1341
BseNI ACTGG 2 cut(s) 626, 740
BseRI GAGGAG 3 cut(s) 138, 141, 156
BseXI GCAGC 4 cut(s) 337, 454, 868, 904
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 832
BshNI GGYRCC 1 cut(s) 1229
BsiHKCI CYCGRG 3 cut(s) 1039, 1165, 1194
BslFI GGGAC 2 cut(s) 99, 991
BslI CCNNNNNNNGG 5 cut(s) 793, 794, 975, 1226, 1342
BsmAI GTCTC 1 cut(s) 1125
BsmBI CGTCTC 1 cut(s) 1125
BsmFI GGGAC 2 cut(s) 99, 991
BsmI GAATGC 2 cut(s) 41, 633
BsnI GGCC 1 cut(s) 832
BsoBI CYCGRG 3 cut(s) 1039, 1165, 1194
Bsp1286I GDGCHC 1 cut(s) 899
Bsp1407I TGTACA 1 cut(s) 505
Bsp143I GATC 5 cut(s) 211, 360, 446, 516, 775
Bsp1720I GCTNAGC 1 cut(s) 668
BspACI CCGC 9 cut(s) 348, 403, 460, 907, 934, 969, 1099, 1321, 1353
BspANI GGCC 1 cut(s) 832
BspCNI CTCAG 2 cut(s) 73, 1340
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 6 cut(s) 777, 979, 980, 1097, 1179, 1231
BspOI GCTAGC 1 cut(s) 599
BspPI GGATC 6 cut(s) 219, 355, 454, 511, 770, 783
BspT107I GGYRCC 1 cut(s) 1229
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 4 cut(s) 414, 943, 1110, 1304
BsrGI TGTACA 1 cut(s) 505
BsrI ACTGG 2 cut(s) 626, 740
BssECI CCNNGG 2 cut(s) 885, 1316
BssMI GATC 5 cut(s) 211, 360, 446, 516, 775
BssSI CACGAG 2 cut(s) 168, 501
Bst2BI CACGAG 2 cut(s) 168, 501
Bst2UI CCWGG 2 cut(s) 291, 1201
Bst4CI ACNGT 3 cut(s) 510, 998, 1363
Bst6I CTCTTC 1 cut(s) 540
BstAFI CTTAAG 1 cut(s) 428
BstAUI TGTACA 1 cut(s) 505
BstC8I GCNNGC 8 cut(s) 405, 465, 559, 597, 601, 1150, 1302, 1378
BstDEI CTNAG 5 cut(s) 81, 668, 1272, 1327, 1338
BstDSI CCRYGG 1 cut(s) 1316
BstF5I GGATG 3 cut(s) 543, 825, 965
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 1 cut(s) 308
BstKTI GATC 5 cut(s) 214, 363, 449, 519, 778
BstMAI GTCTC 1 cut(s) 1125
BstMBI GATC 5 cut(s) 211, 360, 446, 516, 775
BstMWI GCNNNNNNNGC 5 cut(s) 395, 404, 1107, 1149, 1306
BstNI CCWGG 2 cut(s) 291, 1201
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 289, 1199
BstSFI CTRYAG 2 cut(s) 228, 267
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 4 cut(s) 337, 454, 868, 904
BstX2I RGATCY 4 cut(s) 211, 446, 516, 775
BstYI RGATCY 4 cut(s) 211, 446, 516, 775
Bsu36I CCTNAGG 1 cut(s) 1338
BsuI GTATCC 1 cut(s) 1299
BsuRI GGCC 1 cut(s) 832
BtgI CCRYGG 1 cut(s) 1316
BtsCI GGATG 3 cut(s) 543, 825, 965
Cac8I GCNNGC 8 cut(s) 405, 465, 559, 597, 601, 1150, 1302, 1378
CaiI CAGNNNCTG 2 cut(s) 239, 257
CfoI GCGC 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 978, 1081
Csp6I GTAC 3 cut(s) 506, 676, 1230
CviAII CATG 4 cut(s) 584, 772, 792, 1174
CviQI GTAC 3 cut(s) 506, 676, 1230
DdeI CTNAG 5 cut(s) 81, 668, 1272, 1327, 1338
DpnI GATC 5 cut(s) 213, 362, 448, 518, 777
DpnII GATC 5 cut(s) 211, 360, 446, 516, 775
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 899
Eco47I GGWCC 2 cut(s) 978, 1081
Eco57I CTGAAG 2 cut(s) 207, 560
Eco81I CCTNAGG 1 cut(s) 1338
Eco88I CYCGRG 3 cut(s) 1039, 1165, 1194
EcoO109I RGGNCCY 1 cut(s) 978
EcoRII CCWGG 2 cut(s) 289, 1199
EcoT38I GRGCYC 1 cut(s) 899
Esp3I CGTCTC 1 cut(s) 1125
FaeI CATG 4 cut(s) 587, 775, 795, 1177
FaiI YATR 7 cut(s) 371, 488, 585, 718, 773, 793, 1175
FaqI GGGAC 2 cut(s) 99, 991
FatI CATG 4 cut(s) 583, 771, 791, 1173
FauI CCCGC 2 cut(s) 396, 941
Fnu4HI GCNGC 8 cut(s) 348, 351, 461, 468, 882, 918, 1354, 1381
FokI GGATG 3 cut(s) 550, 832, 952
FriOI GRGCYC 1 cut(s) 899
Fsp4HI GCNGC 8 cut(s) 348, 351, 461, 468, 882, 918, 1354, 1381
FspBI CTAG 3 cut(s) 525, 596, 836
GlaI GCGC 1 cut(s) 307
GluI GCNGC 8 cut(s) 348, 351, 461, 468, 882, 918, 1354, 1381
HaeIII GGCC 1 cut(s) 832
HhaI GCGC 1 cut(s) 308
Hin1II CATG 4 cut(s) 587, 775, 795, 1177
Hin6I GCGC 1 cut(s) 306
HinP1I GCGC 1 cut(s) 306
HinfI GANTC 5 cut(s) 53, 493, 531, 550, 1061
HphI GGTGA 3 cut(s) 210, 999, 1146
Hpy166II GTNNAC 2 cut(s) 506, 1254
Hpy188I TCNGA 7 cut(s) 425, 521, 544, 579, 847, 1090, 1130
Hpy188III TCNNGA 6 cut(s) 170, 358, 364, 535, 756, 1165
Hpy8I GTNNAC 2 cut(s) 506, 1254
Hpy99I CGWCG 2 cut(s) 306, 1061
HpyAV CCTTC 5 cut(s) 234, 437, 584, 687, 1241
HpyCH4III ACNGT 3 cut(s) 510, 998, 1363
HpyCH4V TGCA 4 cut(s) 470, 490, 920, 1304
HpyF10VI GCNNNNNNNGC 5 cut(s) 395, 404, 1107, 1149, 1306
HpyF3I CTNAG 5 cut(s) 81, 668, 1272, 1327, 1338
Hsp92II CATG 4 cut(s) 587, 775, 795, 1177
HspAI GCGC 1 cut(s) 306
KflI GGGWCCC 1 cut(s) 978
KpnI GGTACC 1 cut(s) 1233
Kzo9I GATC 5 cut(s) 211, 360, 446, 516, 775
LmnI GCTCC 5 cut(s) 169, 292, 801, 1336, 1373
Lsp1109I GCAGC 4 cut(s) 337, 454, 868, 904
LweI GCATC 2 cut(s) 226, 1274
MaeI CTAG 3 cut(s) 525, 596, 836
MaeIII GTNAC 3 cut(s) 247, 1357, 1363
MalI GATC 5 cut(s) 213, 362, 448, 518, 777
MboI GATC 5 cut(s) 211, 360, 446, 516, 775
MboII GAAGA 9 cut(s) 129, 132, 135, 270, 353, 557, 619, 751, 994
MflI RGATCY 4 cut(s) 211, 446, 516, 775
MhlI GDGCHC 1 cut(s) 899
MluCI AATT 2 cut(s) 74, 661
MlyI GAGTC 2 cut(s) 487, 1055
MmeI TCCRAC 4 cut(s) 779, 1037, 1205, 1316
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 4 cut(s) 33, 429, 456, 903
MspA1I CMGCKG 2 cut(s) 350, 1383
MspCI CTTAAG 1 cut(s) 428
MspR9I CCNGG 2 cut(s) 291, 1201
Mva1269I GAATGC 2 cut(s) 41, 633
MvaI CCWGG 2 cut(s) 291, 1201
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 5 cut(s) 395, 404, 1107, 1149, 1306
NdeII GATC 5 cut(s) 211, 360, 446, 516, 775
NheI GCTAGC 1 cut(s) 595
NlaIII CATG 4 cut(s) 587, 775, 795, 1177
NlaIV GGNNCC 6 cut(s) 777, 979, 980, 1097, 1179, 1231
NmuCI GTSAC 1 cut(s) 1363
NspI RCATGY 1 cut(s) 587
PaeR7I CTCGAG 2 cut(s) 1039, 1194
PctI GAATGC 2 cut(s) 41, 633
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 3 cut(s) 53, 531, 550
PflMI CCANNNNNTGG 1 cut(s) 1226
PkrI GCNGC 8 cut(s) 349, 352, 462, 469, 883, 919, 1355, 1382
PleI GAGTC 2 cut(s) 487, 1055
PpsI GAGTC 2 cut(s) 487, 1055
PpuMI RGGWCCY 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 978
Psp6I CCWGG 2 cut(s) 289, 1199
PspGI CCWGG 2 cut(s) 289, 1199
PspN4I GGNNCC 6 cut(s) 777, 979, 980, 1097, 1179, 1231
PspPI GGNCC 2 cut(s) 978, 1081
PspPPI RGGWCCY 1 cut(s) 978
PspXI VCTCGAGB 2 cut(s) 1039, 1194
PstNI CAGNNNCTG 2 cut(s) 239, 257
PsuI RGATCY 4 cut(s) 211, 446, 516, 775
PvuII CAGCTG 1 cut(s) 1383
RsaI GTAC 3 cut(s) 507, 677, 1231
RsaNI GTAC 3 cut(s) 506, 676, 1230
SaqAI TTAA 4 cut(s) 33, 429, 456, 903
SatI GCNGC 8 cut(s) 348, 351, 461, 468, 882, 918, 1354, 1381
Sau3AI GATC 5 cut(s) 211, 360, 446, 516, 775
Sau96I GGNCC 2 cut(s) 978, 1081
SchI GAGTC 2 cut(s) 487, 1055
ScrFI CCNGG 2 cut(s) 291, 1201
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 2 cut(s) 226, 1274
SfcI CTRYAG 2 cut(s) 228, 267
Sfr274I CTCGAG 2 cut(s) 1039, 1194
SinI GGWCC 2 cut(s) 978, 1081
SlaI CTCGAG 2 cut(s) 1039, 1194
SmlI CTYRAG 5 cut(s) 428, 1021, 1039, 1194, 1205
SmoI CTYRAG 5 cut(s) 428, 1021, 1039, 1194, 1205
Sse9I AATT 2 cut(s) 74, 661
SsiI CCGC 9 cut(s) 348, 403, 460, 907, 934, 969, 1099, 1321, 1353
SspMI CTAG 3 cut(s) 525, 596, 836
StyD4I CCNGG 2 cut(s) 289, 1199
TaaI ACNGT 3 cut(s) 510, 998, 1363
TaqI TCGA 4 cut(s) 65, 319, 1040, 1195
TaqII GACCGA 2 cut(s) 877, 1069
TasI AATT 2 cut(s) 74, 661
TatI WGTACW 1 cut(s) 505
TauI GCSGC 3 cut(s) 350, 463, 1356
TfiI GAWTC 3 cut(s) 53, 531, 550
Tru1I TTAA 4 cut(s) 33, 429, 456, 903
Tru9I TTAA 4 cut(s) 33, 429, 456, 903
TseFI GTSAC 1 cut(s) 1363
TseI GCWGC 5 cut(s) 350, 467, 881, 917, 1380
Tsp45I GTSAC 1 cut(s) 1363
TspDTI ATGAA 4 cut(s) 270, 705, 760, 1110
TspGWI ACGGA 2 cut(s) 217, 1386
Van91I CCANNNNNTGG 1 cut(s) 1226
Vha464I CTTAAG 1 cut(s) 428
VpaK11BI GGWCC 2 cut(s) 978, 1081
XapI RAATTY 1 cut(s) 661
XceI RCATGY 1 cut(s) 587
XhoI CTCGAG 2 cut(s) 1039, 1194
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 3 cut(s) 525, 596, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.