MD12G1123500.v1.1

IQ-DOMAIN 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008563
Physical Location & Seq
Forward (+)
19798246 .. 19802087
3842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1123500.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGGGGAAGAAAGGTTGGTTTTCTTCAGTAAAGAAAGCTCTGAGCCCTGATTCCAAGGAGAAGAAAGAACAGAGATCAGATAAATCGAAAAAGAAATGGTTTGGGAAGCAAAAGTACCCGGAATTCGAGTCTACCTCTTATCAACCATCCACTATATTGCCACCTCTTCCGCCACGAGAAGAGGTGAAATTAACCAATGCGGAGAATGAGCAGAATAATGATGCTTACTCTGTTCCAGTCGCCACCACAGTCTCCACCACTGCAGTGGCTGAGCCTGTGGTTTCTCTGGCTCCTCCGACTCAAGCGGCTGTGGAGGTCGTCAGACTCCCTACAATTACTCAGTATGCTGGGAAATCAAGGGAGGAAGTGGCAGCTATCAAAATTCAAACCGCATTTCGAGGATACCTGGCAAGAAGGGCATTGAGGGCTTTAAGAGGGCTGGTTAGGCTGAAATCATTGATAGAGGGGTCTGTTGTGAAACGGCAAGCCACAAATACCCTCCGATGCATGCAGACTCTATCTCGTGTGCAATCTCAGATACGTTCCAGGAGGATTAGGATGTTAGAAGAGAATCAGGCTCTGCAAAGGCAACTCCTACTAAAACATGCAAAAGAGCTTGAAACTTTGCGGCTCGGGGATGAATGGGATGATAGCATACAGTCGAAGGAGCAAGTTGAAGCTAACCTATTAAGCAAACACGAGGCAACGATGAGAAGGGAAAGAGCACTTGCTTATGCATTTTCTCATCAGAAAAACGGGAAGAATACTACGAAATCTGTAAACCCTATGTTCATGGATCCAAGCAATCCCACCTGGGGTTGGAGCTGGTTGGAAAGGTGGATGGCTGCCCGGCCTTGGGAGAGTCGTGGCATGACGGATAAAGACATGCAAGACGACCATGCATCTGTAAAGAGTGCAAGTCGCGGCATGTCTGCTGGAGAAATTAATAAGTCCTATGCTCGCTACCTCCTGAATTCTGACAAGCAATCCCCAACAGCCAGTGAAAAGCCAAGCCATCCTGGATTCCGGTCCCCTTCAACTCCTTCCAGGCCAGCTTCAAAAGTAGCTCGAAAGTTAAAGCCAGCAGCAAGCCCAAGGGGCAGTAGGGTGCTGGATGATGACACGAAAAGCATGGTGAGTGTCCAATCAACTCAGTTTAGGAGGCACAGCATTGCTGGCTCCTCAGTGAGGGATGACGAAAGCCTAGATAGTTCTCCAGCAGTTCCGAGTTACATGGTACCAACTAAATCTGCAAGAGCTAAGTCCAGGCTGCAGAGCCCAGCAGAGAAGAATGGGATCACTGAGAAGGACTCGCCGCCTGAGTCAGCTAAGAAACGGCTGTCTTTCCCAGCCTCCCCCGCTCGACCAAGGCGGCATTCTGGTCCACCAAGGGTTGACAGCAGTATAATTACAGAAAATAAAACGACCGATATTGGAGTCGAAAGCTAA

Protein Analysis

483

Amino Acids

53.8

Weight (kDa)

10.09

Isoelectric Point (pI)

63.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 124 - 142 5.6e-06 IQ calmodulin-binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1237
AccB1I GGYRCC 1 cut(s) 1237
AccBSI CCGCTC 1 cut(s) 1361
AccI GTMKAC 1 cut(s) 131
AccII CGCG 1 cut(s) 924
AciI CCGC 9 cut(s) 170, 200, 305, 390, 628, 924, 1316, 1359, 1372
AclWI GGATC 3 cut(s) 791, 804, 1304
AcsI RAATTY 3 cut(s) 122, 381, 973
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 2 cut(s) 116, 1239
AfiI CCNNNNNNNGG 5 cut(s) 815, 819, 855, 856, 1188
AgsI TTSAA 5 cut(s) 386, 620, 677, 1038, 1059
AjnI CCWGG 6 cut(s) 405, 545, 812, 1018, 1046, 1265
AjuI GAANNNNNNNTTGG 2 cut(s) 1360, 1392
AleI CACNNNNGTG 1 cut(s) 263
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 3 cut(s) 791, 804, 1304
AlwNI CAGNNNCTG 2 cut(s) 269, 580
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 2 cut(s) 851, 1049
ApeKI GCWGC 4 cut(s) 371, 845, 1085, 1270
ApoI RAATTY 3 cut(s) 122, 381, 973
AseI ATTAAT 1 cut(s) 945
Asp718I GGTACC 1 cut(s) 1237
AspS9I GGNCC 2 cut(s) 1029, 1382
AsuC2I CCSGG 2 cut(s) 119, 850
AsuHPI GGTGA 2 cut(s) 196, 1147
AvaI CYCGRG 1 cut(s) 632
AvaII GGWCC 2 cut(s) 1029, 1382
BamHI GGATCC 1 cut(s) 796
BanI GGYRCC 1 cut(s) 1237
BanII GRGCYC 2 cut(s) 47, 1280
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BauI CACGAG 3 cut(s) 174, 522, 698
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 4 cut(s) 383, 832, 1097, 1257
BccI CCATC 3 cut(s) 154, 835, 1023
BceAI ACGGC 2 cut(s) 497, 1352
BciT130I CCWGG 6 cut(s) 407, 547, 814, 1020, 1048, 1267
BciVI GTATCC 1 cut(s) 395
BcnI CCSGG 2 cut(s) 119, 850
BcoDI GTCTC 1 cut(s) 256
BfaI CTAG 1 cut(s) 1205
BfmI CTRYAG 2 cut(s) 261, 1271
BfuI GTATCC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 1098
BisI GCNGC 9 cut(s) 306, 372, 629, 846, 925, 1086, 1271, 1316, 1373
BlpI GCTNAGC 1 cut(s) 270
BlsI GCNGC 9 cut(s) 307, 373, 630, 847, 926, 1087, 1272, 1317, 1374
Bme1390I CCNGG 8 cut(s) 119, 407, 547, 814, 850, 1020, 1048, 1267
Bme18I GGWCC 2 cut(s) 1029, 1382
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 1029, 1382
BmiI GGNNCC 5 cut(s) 291, 798, 1031, 1180, 1239
BmrFI CCNGG 8 cut(s) 119, 407, 547, 814, 850, 1020, 1048, 1267
BmsI GCATC 3 cut(s) 211, 494, 911
BplI GAGNNNNNCTC 4 cut(s) 119, 151, 1295, 1327
BpmI CTGGAG 2 cut(s) 957, 1200
Bpu1102I GCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 119, 850
BsaJI CCNNGG 6 cut(s) 54, 813, 854, 1094, 1367, 1388
BsaWI WCCGGW 1 cut(s) 1026
Bsc4I CCNNNNNNNGG 5 cut(s) 815, 819, 855, 856, 1188
Bse1I ACTGG 2 cut(s) 236, 999
Bse3DI GCAATG 1 cut(s) 1170
BseBI CCWGG 6 cut(s) 407, 547, 814, 1020, 1048, 1267
BseDI CCNNGG 6 cut(s) 54, 813, 854, 1094, 1367, 1388
BseGI GGATG 8 cut(s) 146, 564, 643, 652, 846, 1015, 1120, 1198
BseLI CCNNNNNNNGG 5 cut(s) 815, 819, 855, 856, 1188
BseMI GCAATG 1 cut(s) 1170
BseMII CTCAG 8 cut(s) 32, 261, 353, 548, 1166, 1197, 1293, 1311
BseNI ACTGG 2 cut(s) 236, 999
BseRI GAGGAG 2 cut(s) 282, 1171
BseXI GCAGC 4 cut(s) 383, 832, 1097, 1257
BseYI CCCAGC 3 cut(s) 347, 1279, 1348
Bsh1236I CGCG 1 cut(s) 924
Bsh1285I CGRYCG 1 cut(s) 1428
BshFI GGCC 2 cut(s) 853, 1051
BshNI GGYRCC 1 cut(s) 1237
BsiEI CGRYCG 1 cut(s) 1428
BsiHKAI GWGCWC 1 cut(s) 727
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 3 cut(s) 119, 850, 1027
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 5 cut(s) 815, 819, 855, 856, 1188
BsmAI GTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 1015
BsmI GAATGC 1 cut(s) 1375
BsnI GGCC 2 cut(s) 853, 1051
BsoBI CYCGRG 1 cut(s) 632
Bsp1286I GDGCHC 3 cut(s) 47, 727, 1280
Bsp143I GATC 3 cut(s) 74, 796, 1296
Bsp1720I GCTNAGC 1 cut(s) 270
BspACI CCGC 9 cut(s) 170, 200, 305, 390, 628, 924, 1316, 1359, 1372
BspANI GGCC 2 cut(s) 853, 1051
BspCNI CTCAG 8 cut(s) 33, 262, 352, 547, 1165, 1196, 1294, 1312
BspFNI CGCG 1 cut(s) 924
BspLI GGNNCC 5 cut(s) 291, 798, 1031, 1180, 1239
BspMAI CTGCAG 2 cut(s) 265, 1275
BspPI GGATC 3 cut(s) 791, 804, 1304
BspT107I GGYRCC 1 cut(s) 1237
BsrBI CCGCTC 1 cut(s) 1361
BsrDI GCAATG 1 cut(s) 1170
BsrI ACTGG 2 cut(s) 236, 999
BssECI CCNNGG 6 cut(s) 54, 813, 854, 1094, 1367, 1388
BssMI GATC 3 cut(s) 74, 796, 1296
BssSI CACGAG 3 cut(s) 174, 522, 698
BssT1I CCWWGG 5 cut(s) 54, 854, 1094, 1367, 1388
Bst2BI CACGAG 3 cut(s) 174, 522, 698
Bst2UI CCWGG 6 cut(s) 407, 547, 814, 1020, 1048, 1267
Bst4CI ACNGT 2 cut(s) 250, 660
Bst6I CTCTTC 3 cut(s) 171, 174, 561
BstC8I GCNNGC 7 cut(s) 486, 509, 961, 1053, 1083, 1090, 1177
BstENI CCTNNNNNAGG 1 cut(s) 1186
BstF5I GGATG 8 cut(s) 146, 564, 643, 652, 846, 1015, 1120, 1198
BstFNI CGCG 1 cut(s) 924
BstKTI GATC 3 cut(s) 77, 799, 1299
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 3 cut(s) 74, 796, 1296
BstMCI CGRYCG 1 cut(s) 1428
BstMWI GCNNNNNNNGC 6 cut(s) 416, 425, 445, 1098, 1176, 1358
BstNI CCWGG 6 cut(s) 407, 547, 814, 1020, 1048, 1267
BstNSI RCATGY 4 cut(s) 511, 608, 889, 931
BstSCI CCNGG 8 cut(s) 117, 405, 545, 812, 848, 1018, 1046, 1265
BstSFI CTRYAG 2 cut(s) 261, 1271
BstUI CGCG 1 cut(s) 924
BstV1I GCAGC 4 cut(s) 383, 832, 1097, 1257
BstX2I RGATCY 1 cut(s) 796
BstXI CCANNNNNNTGG 1 cut(s) 265
BstYI RGATCY 1 cut(s) 796
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 2 cut(s) 853, 1051
BtsCI GGATG 8 cut(s) 146, 564, 643, 652, 846, 1015, 1120, 1198
BtsI GCAGTG 2 cut(s) 258, 270
BtsIMutI CAGTG 5 cut(s) 258, 270, 1006, 1191, 1299
Cac8I GCNNGC 7 cut(s) 486, 509, 961, 1053, 1083, 1090, 1177
CaiI CAGNNNCTG 2 cut(s) 269, 580
Cfr13I GGNCC 2 cut(s) 1029, 1382
Csp6I GTAC 2 cut(s) 115, 1238
CviAII CATG 9 cut(s) 508, 605, 793, 871, 886, 899, 928, 1132, 1234
CviQI GTAC 2 cut(s) 115, 1238
DpnI GATC 3 cut(s) 76, 798, 1298
DpnII GATC 3 cut(s) 74, 796, 1296
Eam1104I CTCTTC 3 cut(s) 171, 174, 561
EarI CTCTTC 3 cut(s) 171, 174, 561
EciI GGCGGA 1 cut(s) 159
Eco130I CCWWGG 5 cut(s) 54, 854, 1094, 1367, 1388
Eco24I GRGCYC 2 cut(s) 47, 1280
Eco47I GGWCC 2 cut(s) 1029, 1382
Eco57I CTGAAG 1 cut(s) 9
Eco88I CYCGRG 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 1186
EcoRI GAATTC 2 cut(s) 122, 973
EcoRII CCWGG 6 cut(s) 405, 545, 812, 1018, 1046, 1265
EcoT14I CCWWGG 5 cut(s) 54, 854, 1094, 1367, 1388
EcoT22I ATGCAT 3 cut(s) 509, 739, 904
EcoT38I GRGCYC 2 cut(s) 47, 1280
ErhI CCWWGG 5 cut(s) 54, 854, 1094, 1367, 1388
FaeI CATG 9 cut(s) 511, 608, 796, 874, 889, 902, 931, 1135, 1237
FaqI GGGAC 1 cut(s) 1015
FatI CATG 9 cut(s) 507, 604, 792, 870, 885, 898, 927, 1131, 1233
FauI CCCGC 1 cut(s) 1366
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 9 cut(s) 306, 372, 629, 846, 925, 1086, 1271, 1316, 1373
FokI GGATG 8 cut(s) 133, 571, 650, 659, 853, 1002, 1127, 1205
FriOI GRGCYC 2 cut(s) 47, 1280
Fsp4HI GCNGC 9 cut(s) 306, 372, 629, 846, 925, 1086, 1271, 1316, 1373
FspBI CTAG 1 cut(s) 1205
GluI GCNGC 9 cut(s) 306, 372, 629, 846, 925, 1086, 1271, 1316, 1373
GsaI CCCAGC 3 cut(s) 351, 1283, 1352
GsuI CTGGAG 2 cut(s) 957, 1200
HaeIII GGCC 2 cut(s) 853, 1051
HapII CCGG 3 cut(s) 119, 850, 1027
Hin1II CATG 9 cut(s) 511, 608, 796, 874, 889, 902, 931, 1135, 1237
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HpaII CCGG 3 cut(s) 119, 850, 1027
HphI GGTGA 2 cut(s) 196, 1147
Hpy166II GTNNAC 4 cut(s) 132, 781, 1385, 1396
Hpy188I TCNGA 9 cut(s) 42, 79, 297, 323, 503, 537, 750, 979, 1227
Hpy188III TCNNGA 1 cut(s) 970
Hpy8I GTNNAC 4 cut(s) 132, 781, 1385, 1396
HpyAV CCTTC 6 cut(s) 408, 658, 708, 1044, 1053, 1300
HpyCH4III ACNGT 2 cut(s) 250, 660
HpyCH4IV ACGT 1 cut(s) 541
HpyF10VI GCNNNNNNNGC 6 cut(s) 416, 425, 445, 1098, 1176, 1358
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 9 cut(s) 511, 608, 796, 874, 889, 902, 931, 1135, 1237
KpnI GGTACC 1 cut(s) 1241
Kzo9I GATC 3 cut(s) 74, 796, 1296
LmnI GCTCC 4 cut(s) 295, 667, 822, 1184
Lsp1109I GCAGC 4 cut(s) 383, 832, 1097, 1257
LweI GCATC 3 cut(s) 211, 494, 911
MaeI CTAG 1 cut(s) 1205
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 1 cut(s) 1229
MalI GATC 3 cut(s) 76, 798, 1298
MbiI CCGCTC 1 cut(s) 1361
MboI GATC 3 cut(s) 74, 796, 1296
MboII GAAGA 8 cut(s) 15, 19, 73, 158, 191, 578, 772, 1300
MflI RGATCY 1 cut(s) 796
MhlI GDGCHC 3 cut(s) 47, 727, 1280
MluCI AATT 7 cut(s) 122, 188, 333, 381, 942, 973, 1407
MlyI GAGTC 8 cut(s) 137, 292, 318, 508, 871, 1304, 1331, 1446
MmeI TCCRAC 3 cut(s) 320, 800, 810
Mph1103I ATGCAT 3 cut(s) 509, 739, 904
MseI TTAA 5 cut(s) 191, 431, 689, 945, 1076
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 3 cut(s) 119, 850, 1027
MspR9I CCNGG 8 cut(s) 119, 407, 547, 814, 850, 1020, 1048, 1267
Mva1269I GAATGC 1 cut(s) 1375
MvaI CCWGG 6 cut(s) 407, 547, 814, 1020, 1048, 1267
MvnI CGCG 1 cut(s) 924
MwoI GCNNNNNNNGC 6 cut(s) 416, 425, 445, 1098, 1176, 1358
NciI CCSGG 2 cut(s) 119, 850
NdeII GATC 3 cut(s) 74, 796, 1296
NlaIII CATG 9 cut(s) 511, 608, 796, 874, 889, 902, 931, 1135, 1237
NlaIV GGNNCC 5 cut(s) 291, 798, 1031, 1180, 1239
NsiI ATGCAT 3 cut(s) 509, 739, 904
NspI RCATGY 4 cut(s) 511, 608, 889, 931
OliI CACNNNNGTG 1 cut(s) 263
PaeI GCATGC 1 cut(s) 511
PctI GAATGC 1 cut(s) 1375
PfeI GAWTC 3 cut(s) 50, 571, 1023
PfoI TCCNGGA 2 cut(s) 545, 1018
PkrI GCNGC 9 cut(s) 307, 373, 630, 847, 926, 1087, 1272, 1317, 1374
PleI GAGTC 8 cut(s) 136, 292, 318, 508, 870, 1304, 1330, 1445
PpsI GAGTC 8 cut(s) 136, 292, 318, 508, 870, 1304, 1330, 1445
PshBI ATTAAT 1 cut(s) 945
Psp6I CCWGG 6 cut(s) 405, 545, 812, 1018, 1046, 1265
PspFI CCCAGC 3 cut(s) 347, 1279, 1348
PspGI CCWGG 6 cut(s) 405, 545, 812, 1018, 1046, 1265
PspN4I GGNNCC 5 cut(s) 291, 798, 1031, 1180, 1239
PspPI GGNCC 2 cut(s) 1029, 1382
PstI CTGCAG 2 cut(s) 265, 1275
PstNI CAGNNNCTG 2 cut(s) 269, 580
PsuI RGATCY 1 cut(s) 796
RsaI GTAC 2 cut(s) 116, 1239
RsaNI GTAC 2 cut(s) 115, 1238
RseI CAYNNNNRTG 1 cut(s) 263
SaqAI TTAA 5 cut(s) 191, 431, 689, 945, 1076
SatI GCNGC 9 cut(s) 306, 372, 629, 846, 925, 1086, 1271, 1316, 1373
Sau3AI GATC 3 cut(s) 74, 796, 1296
Sau96I GGNCC 2 cut(s) 1029, 1382
SchI GAGTC 8 cut(s) 137, 292, 318, 508, 871, 1304, 1331, 1446
ScrFI CCNGG 8 cut(s) 119, 407, 547, 814, 850, 1020, 1048, 1267
SduI GDGCHC 3 cut(s) 47, 727, 1280
SfaNI GCATC 3 cut(s) 211, 494, 911
SfcI CTRYAG 2 cut(s) 261, 1271
SinI GGWCC 2 cut(s) 1029, 1382
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SphI GCATGC 1 cut(s) 511
Sse9I AATT 7 cut(s) 122, 188, 333, 381, 942, 973, 1407
SsiI CCGC 9 cut(s) 170, 200, 305, 390, 628, 924, 1316, 1359, 1372
SspMI CTAG 1 cut(s) 1205
StyD4I CCNGG 8 cut(s) 117, 405, 545, 812, 848, 1018, 1046, 1265
StyI CCWWGG 5 cut(s) 54, 854, 1094, 1367, 1388
TaaI ACNGT 2 cut(s) 250, 660
TaiI ACGT 1 cut(s) 544
TaqI TCGA 7 cut(s) 86, 126, 397, 662, 1069, 1363, 1440
TaqII GACCGA 1 cut(s) 1442
TasI AATT 7 cut(s) 122, 188, 333, 381, 942, 973, 1407
TauI GCSGC 5 cut(s) 308, 631, 927, 1318, 1375
TfiI GAWTC 3 cut(s) 50, 571, 1023
Tru1I TTAA 5 cut(s) 191, 431, 689, 945, 1076
Tru9I TTAA 5 cut(s) 191, 431, 689, 945, 1076
TscAI CASTG 5 cut(s) 265, 270, 1006, 1191, 1306
TseI GCWGC 4 cut(s) 371, 845, 1085, 1270
TspDTI ATGAA 2 cut(s) 654, 781
TspGWI ACGGA 1 cut(s) 890
TspRI CASTG 5 cut(s) 265, 270, 1006, 1191, 1306
VpaK11BI GGWCC 2 cut(s) 1029, 1382
VspI ATTAAT 1 cut(s) 945
XagI CCTNNNNNAGG 1 cut(s) 1186
XapI RAATTY 3 cut(s) 122, 381, 973
XceI RCATGY 4 cut(s) 511, 608, 889, 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XmiI GTMKAC 1 cut(s) 131
XspI CTAG 1 cut(s) 1205
Zsp2I ATGCAT 3 cut(s) 509, 739, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.