Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008563

Physical Location & Seq

Genomic Coordinates Forward (+)

19096474 .. 19100543

Gene Structure 4070 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1103600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGGGGAAGAAAGGTTGGTTTTCTTCAGTAAAGAAAGCTCTGAGCCCTGATTCTAAGAAGAAAGAGCAGAGATCAGATCAATCAAAAAAGAAATGGTTTGGGAAACAAAAGTACCCAGAGTTTGAGTCTACTTCTTATCAACCGTCCATTATATTGCCACCTCTTCCACCACGAGAAGAGGTGAAATTAACCAATGTGGAGAATGAGCACAACAATGATGCTTACTCTGTTCCAGTCACCACCACAGTCACCCCTACCGCAGTGGCTGAGGCTGCAGTTTCTGTGGCTCCTCCAACTCAAGCTGTTGTGGAGGTTGTCAGACTCCCTACAACTACTCAGTATGCTGGAAAATCAAGGGAGGAAGTGGCAGCAATCAAGATTCAAACGGCATTTCGAGGATACCTGGCAAGAAGGGCGTTGAGGGCGTTAAGAGGGCTTGTTAGGCTGAAATCATTGATAGAGGGATCTGTTGTGAAACGGCAAGCCACAAATACCCTCCGATGCATGCAGACTCTATCTCGTGTGCAGTCTCAGATACGTTCCAGGAGGATTAGGATGTTAGAAGAGAATCAGGCTCTGCAAAGGCAACTCCTACTAAAACATGCAAAAGAGCTTGAGACATTGCGGCTTGGGGATGAATGGGATGATAGCATACAGTCAAAGGAGCAAGTTGAAGCAAACCTATTAAGCAAACACGAGGCAGCAATGAGAAGGGAAAGAGCACTCGCTTATGCATTCTCTCATCAGAAAAATGGGAAGAATACTACAAAATCTGTAAACCCAATGTTCATGGATCCAAGCAATCCCACATGGGGTTGGAGCTGGTTGGAAAGGTGGATGGCTGCCCGGCCTTGGGAGAGTCGTGGCATGACAGATAAAGACATGCAGGATGACCATGCATCTGTAAAGAGTGCAAGTCGCGGCATTTCTGCTGGAGAAATTAACAAGTCCTATGCTCGCTACCTCCTCAATTCCGACAAGCAATCCCCAACAGCCAGTGAAAAGCCAAGCCATCCTGGATTTCGGTCCCCTTCAAGTCCTTCTAGGCCAGCTTCGAAAGTAGCTCGAAAATTAAAGCCAGCAGCAAGCCCAAGGGGTAGTAGGGTCCCAGATTCTGACACGAAAAGCATGGTCAGTGTGCAATCAACTCAGTTAAGGAGGCACAGCATTGCTGGCTCCTCTGTGAGGGATGACGAAAGCCTAGATAGTTCTCCAGCAGTTCCAAGTTACATGGTACCAACAAAATCCGCAAGAGCTAAGTCCAGGCTGCAGAGCCCAGCAGAGAAGAATGGGATCACCGAGAAGGACTTGATGCCTGAGTCAGCTAAGAAACGGCTGTCATTCCCAGCCTCCCCCGCGCGACCAAGGCGGCATTCTGGTCCACCAAGGGTTGACAGCAGTATAATTACAGCAAACAAAGTGAGTGATATTGGAGTCGAAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005856 GO:0005874 GO:0008017 GO:0008092 GO:0015630 GO:0015631 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4005 IQ

Protein Analysis

482

Amino Acids

53.55

Weight (kDa)

10.18

Isoelectric Point (pI)

63.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IQ	PF00612	123 - 141	5.6e-06	IQ calmodulin-binding motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008563)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52290 AT5G03040 AT5G03040 AT5G03040
fragaria_vesca	FvH4_6g15440 FvH4_6g15440
malus_domestica	MD04G1103600.v1.1 MD12G1123500.v1.1
prunus_persica	Prupe.6G239700_v2.0.a1
pyrus_communis	pycom04g09880 pycom12g12220
rosa_chinensis	RchiOBHm_Chr3g0468921
rosa_laevigata	RLG00000024400
rosa_multiflora	Rmu_co8378931.1_g000001 Rmu_sc0001639.1_g000010 Rmu_sc0003298.1_g000001
rosa_roxburghii	Rroxscaffold_6G00412460
rosa_rugosa	Rorug03G0097600 Rorug03G0097600
rosa_samantha	Rh3AG147200 Rh3BG169800 Rh3DG205300
rosa_wichuraiana	Rw3G013850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1234
AccB1I	GGYRCC	1 cut(s)	1234
AccI	GTMKAC	1 cut(s)	128
AccII	CGCG	3 cut(s)	921, 1358, 1360
AciI	CCGC	6 cut(s)	258, 625, 921, 1248, 1356, 1369
AclWI	GGATC	4 cut(s)	472, 788, 801, 1301
AcuI	CTGAAG	1 cut(s)	9
AfaI	GTAC	2 cut(s)	113, 1236
AfiI	CCNNNNNNNGG	4 cut(s)	812, 852, 853, 1185
AgsI	TTSAA	3 cut(s)	383, 674, 1035
AjnI	CCWGG	4 cut(s)	402, 542, 1015, 1262
AjuI	GAANNNNNNNTTGG	2 cut(s)	1357, 1389
AluBI	AGCT	9 cut(s)	38, 302, 613, 822, 1052, 1064, 1256, 1325, 1443
AluI	AGCT	9 cut(s)	38, 302, 613, 822, 1052, 1064, 1256, 1325, 1443
Alw21I	GWGCWC	2 cut(s)	210, 724
Alw26I	GTCTC	2 cut(s)	534, 611
AlwI	GGATC	4 cut(s)	472, 788, 801, 1301
AlwNI	CAGNNNCTG	4 cut(s)	266, 281, 577, 1115
AoxI	GGCC	2 cut(s)	848, 1046
ApeKI	GCWGC	6 cut(s)	272, 368, 701, 842, 1082, 1267
Asp718I	GGTACC	1 cut(s)	1234
AspLEI	GCGC	1 cut(s)	1360
AspS9I	GGNCC	3 cut(s)	1026, 1105, 1379
AsuC2I	CCSGG	1 cut(s)	847
AsuHPI	GGTGA	4 cut(s)	193, 229, 241, 1288
AsuII	TTCGAA	1 cut(s)	1055
AvaII	GGWCC	3 cut(s)	1026, 1105, 1379
BaeI	ACNNNNGTAYC	2 cut(s)	95, 128
BamHI	GGATCC	1 cut(s)	793
BanI	GGYRCC	1 cut(s)	1234
BanII	GRGCYC	2 cut(s)	47, 1277
BauI	CACGAG	3 cut(s)	171, 519, 695
Bbv12I	GWGCWC	2 cut(s)	210, 724
BbvCI	CCTCAGC	1 cut(s)	267
BbvI	GCAGC	6 cut(s)	259, 380, 713, 829, 1094, 1254
BccI	CCATC	2 cut(s)	832, 1020
BceAI	ACGGC	3 cut(s)	402, 494, 1349
BciT130I	CCWGG	4 cut(s)	404, 544, 1017, 1264
BciVI	GTATCC	1 cut(s)	392
BcnI	CCSGG	1 cut(s)	847
BcoDI	GTCTC	2 cut(s)	534, 611
BfaI	CTAG	2 cut(s)	1044, 1202
BfmI	CTRYAG	2 cut(s)	273, 1268
BfuI	GTATCC	1 cut(s)	392
BisI	GCNGC	9 cut(s)	273, 369, 626, 702, 843, 922, 1083, 1268, 1370
BlsI	GCNGC	9 cut(s)	274, 370, 627, 703, 844, 923, 1084, 1269, 1371
Bme1390I	CCNGG	5 cut(s)	404, 544, 847, 1017, 1264
Bme18I	GGWCC	3 cut(s)	1026, 1105, 1379
BmgT120I	GGNCC	3 cut(s)	1026, 1105, 1379
BmiI	GGNNCC	7 cut(s)	288, 795, 1028, 1106, 1107, 1177, 1236
BmrFI	CCNGG	5 cut(s)	404, 544, 847, 1017, 1264
BmsI	GCATC	4 cut(s)	208, 491, 908, 1302
BpmI	CTGGAG	2 cut(s)	954, 1197
Bpu10I	CCTNAGC	1 cut(s)	267
Bpu14I	TTCGAA	1 cut(s)	1055
BpuEI	CTTGAG	2 cut(s)	282, 635
BpuMI	CCSGG	1 cut(s)	847
BsaJI	CCNNGG	4 cut(s)	851, 1091, 1364, 1385
Bsc4I	CCNNNNNNNGG	4 cut(s)	812, 852, 853, 1185
Bse1I	ACTGG	2 cut(s)	233, 996
Bse3DI	GCAATG	3 cut(s)	620, 711, 1167
BseBI	CCWGG	4 cut(s)	404, 544, 1017, 1264
BseDI	CCNNGG	4 cut(s)	851, 1091, 1364, 1385
BseGI	GGATG	7 cut(s)	561, 640, 649, 843, 895, 1012, 1195
BseLI	CCNNNNNNNGG	4 cut(s)	812, 852, 853, 1185
BseMI	GCAATG	3 cut(s)	620, 711, 1167
BseMII	CTCAG	6 cut(s)	32, 258, 350, 545, 1163, 1308
BseNI	ACTGG	2 cut(s)	233, 996
BseRI	GAGGAG	3 cut(s)	279, 956, 1168
BseXI	GCAGC	6 cut(s)	259, 380, 713, 829, 1094, 1254
BseYI	CCCAGC	2 cut(s)	1276, 1345
BsgI	GTGCAG	1 cut(s)	545
Bsh1236I	CGCG	3 cut(s)	921, 1358, 1360
BshFI	GGCC	2 cut(s)	850, 1048
BshNI	GGYRCC	1 cut(s)	1234
BsiHKAI	GWGCWC	2 cut(s)	210, 724
BsiSI	CCGG	1 cut(s)	847
BslFI	GGGAC	2 cut(s)	1012, 1091
BslI	CCNNNNNNNGG	4 cut(s)	812, 852, 853, 1185
BsmAI	GTCTC	2 cut(s)	534, 611
BsmFI	GGGAC	2 cut(s)	1012, 1091
BsmI	GAATGC	2 cut(s)	734, 1372
BsnI	GGCC	2 cut(s)	850, 1048
Bsp119I	TTCGAA	1 cut(s)	1055
Bsp1286I	GDGCHC	4 cut(s)	47, 210, 724, 1277
Bsp143I	GATC	5 cut(s)	71, 76, 464, 793, 1293
BspACI	CCGC	6 cut(s)	258, 625, 921, 1248, 1356, 1369
BspANI	GGCC	2 cut(s)	850, 1048
BspCNI	CTCAG	6 cut(s)	33, 259, 349, 544, 1162, 1309
BspFNI	CGCG	3 cut(s)	921, 1358, 1360
BspLI	GGNNCC	7 cut(s)	288, 795, 1028, 1106, 1107, 1177, 1236
BspMAI	CTGCAG	2 cut(s)	277, 1272
BspPI	GGATC	4 cut(s)	472, 788, 801, 1301
BspT104I	TTCGAA	1 cut(s)	1055
BspT107I	GGYRCC	1 cut(s)	1234
BsrDI	GCAATG	3 cut(s)	620, 711, 1167
BsrI	ACTGG	2 cut(s)	233, 996
BssECI	CCNNGG	4 cut(s)	851, 1091, 1364, 1385
BssMI	GATC	5 cut(s)	71, 76, 464, 793, 1293
BssSI	CACGAG	3 cut(s)	171, 519, 695
BssT1I	CCWWGG	4 cut(s)	851, 1091, 1364, 1385
Bst2BI	CACGAG	3 cut(s)	171, 519, 695
Bst2UI	CCWGG	4 cut(s)	404, 544, 1017, 1264
Bst4CI	ACNGT	3 cut(s)	144, 247, 657
Bst6I	CTCTTC	3 cut(s)	168, 171, 558
BstBI	TTCGAA	1 cut(s)	1055
BstC8I	GCNNGC	7 cut(s)	483, 506, 958, 1050, 1080, 1087, 1174
BstDEI	CTNAG	9 cut(s)	41, 54, 267, 336, 531, 1149, 1257, 1317, 1326
BstENI	CCTNNNNNAGG	1 cut(s)	1183
BstF5I	GGATG	7 cut(s)	561, 640, 649, 843, 895, 1012, 1195
BstFNI	CGCG	3 cut(s)	921, 1358, 1360
BstHHI	GCGC	1 cut(s)	1360
BstKTI	GATC	5 cut(s)	74, 79, 467, 796, 1296
BstMAI	GTCTC	2 cut(s)	534, 611
BstMBI	GATC	5 cut(s)	71, 76, 464, 793, 1293
BstMWI	GCNNNNNNNGC	7 cut(s)	272, 413, 422, 442, 1173, 1355, 1366
BstNI	CCWGG	4 cut(s)	404, 544, 1017, 1264
BstNSI	RCATGY	3 cut(s)	508, 605, 886
BstSCI	CCNGG	5 cut(s)	402, 542, 845, 1015, 1262
BstSFI	CTRYAG	2 cut(s)	273, 1268
BstUI	CGCG	3 cut(s)	921, 1358, 1360
BstV1I	GCAGC	6 cut(s)	259, 380, 713, 829, 1094, 1254
BstX2I	RGATCY	2 cut(s)	464, 793
BstYI	RGATCY	2 cut(s)	464, 793
BsuI	GTATCC	1 cut(s)	392
BsuRI	GGCC	2 cut(s)	850, 1048
BtsCI	GGATG	7 cut(s)	561, 640, 649, 843, 895, 1012, 1195
BtsI	GCAGTG	1 cut(s)	267
BtsIMutI	CAGTG	3 cut(s)	267, 1003, 1141
Cac8I	GCNNGC	7 cut(s)	483, 506, 958, 1050, 1080, 1087, 1174
CaiI	CAGNNNCTG	4 cut(s)	266, 281, 577, 1115
CfoI	GCGC	1 cut(s)	1360
Cfr13I	GGNCC	3 cut(s)	1026, 1105, 1379
Csp6I	GTAC	2 cut(s)	112, 1235
CviAII	CATG	9 cut(s)	505, 602, 790, 810, 868, 883, 896, 1129, 1231
CviQI	GTAC	2 cut(s)	112, 1235
DdeI	CTNAG	9 cut(s)	41, 54, 267, 336, 531, 1149, 1257, 1317, 1326
DpnI	GATC	5 cut(s)	73, 78, 466, 795, 1295
DpnII	GATC	5 cut(s)	71, 76, 464, 793, 1293
Eam1104I	CTCTTC	3 cut(s)	168, 171, 558
EarI	CTCTTC	3 cut(s)	168, 171, 558
Eco130I	CCWWGG	4 cut(s)	851, 1091, 1364, 1385
Eco24I	GRGCYC	2 cut(s)	47, 1277
Eco47I	GGWCC	3 cut(s)	1026, 1105, 1379
Eco57I	CTGAAG	1 cut(s)	9
EcoNI	CCTNNNNNAGG	1 cut(s)	1183
EcoO109I	RGGNCCY	1 cut(s)	1105
EcoRII	CCWGG	4 cut(s)	402, 542, 1015, 1262
EcoT14I	CCWWGG	4 cut(s)	851, 1091, 1364, 1385
EcoT22I	ATGCAT	3 cut(s)	506, 736, 901
EcoT38I	GRGCYC	2 cut(s)	47, 1277
ErhI	CCWWGG	4 cut(s)	851, 1091, 1364, 1385
FaeI	CATG	9 cut(s)	508, 605, 793, 813, 871, 886, 899, 1132, 1234
FaqI	GGGAC	2 cut(s)	1012, 1091
FatI	CATG	9 cut(s)	504, 601, 789, 809, 867, 882, 895, 1128, 1230
FauI	CCCGC	1 cut(s)	1363
FblI	GTMKAC	1 cut(s)	128
Fnu4HI	GCNGC	9 cut(s)	273, 369, 626, 702, 843, 922, 1083, 1268, 1370
FokI	GGATG	7 cut(s)	568, 647, 656, 850, 902, 999, 1202
FriOI	GRGCYC	2 cut(s)	47, 1277
Fsp4HI	GCNGC	9 cut(s)	273, 369, 626, 702, 843, 922, 1083, 1268, 1370
FspBI	CTAG	2 cut(s)	1044, 1202
GlaI	GCGC	1 cut(s)	1359
GluI	GCNGC	9 cut(s)	273, 369, 626, 702, 843, 922, 1083, 1268, 1370
GsaI	CCCAGC	2 cut(s)	1280, 1349
GsuI	CTGGAG	2 cut(s)	954, 1197
HaeIII	GGCC	2 cut(s)	850, 1048
HapII	CCGG	1 cut(s)	847
HhaI	GCGC	1 cut(s)	1360
Hin1II	CATG	9 cut(s)	508, 605, 793, 813, 871, 886, 899, 1132, 1234
Hin6I	GCGC	1 cut(s)	1358
HinP1I	GCGC	1 cut(s)	1358
HincII	GTYRAC	1 cut(s)	1393
HindII	GTYRAC	1 cut(s)	1393
HpaII	CCGG	1 cut(s)	847
HphI	GGTGA	4 cut(s)	193, 229, 241, 1288
Hpy166II	GTNNAC	4 cut(s)	129, 778, 1382, 1393
Hpy188I	TCNGA	8 cut(s)	42, 76, 320, 500, 534, 747, 976, 1117
Hpy188III	TCNNGA	1 cut(s)	376
Hpy8I	GTNNAC	4 cut(s)	129, 778, 1382, 1393
HpyAV	CCTTC	5 cut(s)	405, 705, 1041, 1050, 1297
HpyCH4III	ACNGT	3 cut(s)	144, 247, 657
HpyCH4IV	ACGT	1 cut(s)	538
HpyF10VI	GCNNNNNNNGC	7 cut(s)	272, 413, 422, 442, 1173, 1355, 1366
HpyF3I	CTNAG	9 cut(s)	41, 54, 267, 336, 531, 1149, 1257, 1317, 1326
HpySE526I	ACGT	1 cut(s)	538
Hsp92II	CATG	9 cut(s)	508, 605, 793, 813, 871, 886, 899, 1132, 1234
HspAI	GCGC	1 cut(s)	1358
KflI	GGGWCCC	1 cut(s)	1105
KpnI	GGTACC	1 cut(s)	1238
Kzo9I	GATC	5 cut(s)	71, 76, 464, 793, 1293
LmnI	GCTCC	4 cut(s)	292, 664, 819, 1181
Lsp1109I	GCAGC	6 cut(s)	259, 380, 713, 829, 1094, 1254
LweI	GCATC	4 cut(s)	208, 491, 908, 1302
MaeI	CTAG	2 cut(s)	1044, 1202
MaeII	ACGT	1 cut(s)	538
MaeIII	GTNAC	3 cut(s)	235, 247, 1226
MalI	GATC	5 cut(s)	73, 78, 466, 795, 1295
MboI	GATC	5 cut(s)	71, 76, 464, 793, 1293
MboII	GAAGA	8 cut(s)	15, 19, 70, 155, 188, 575, 769, 1297
MflI	RGATCY	2 cut(s)	464, 793
MhlI	GDGCHC	4 cut(s)	47, 210, 724, 1277
MluCI	AATT	5 cut(s)	185, 939, 970, 1070, 1404
MlyI	GAGTC	6 cut(s)	134, 315, 505, 868, 1328, 1443
MmeI	TCCRAC	4 cut(s)	317, 797, 807, 999
Mph1103I	ATGCAT	3 cut(s)	506, 736, 901
MseI	TTAA	6 cut(s)	188, 428, 686, 942, 1073, 1154
MslI	CAYNNNNRTG	1 cut(s)	213
MspI	CCGG	1 cut(s)	847
MspR9I	CCNGG	5 cut(s)	404, 544, 847, 1017, 1264
Mva1269I	GAATGC	2 cut(s)	734, 1372
MvaI	CCWGG	4 cut(s)	404, 544, 1017, 1264
MvnI	CGCG	3 cut(s)	921, 1358, 1360
MwoI	GCNNNNNNNGC	7 cut(s)	272, 413, 422, 442, 1173, 1355, 1366
NciI	CCSGG	1 cut(s)	847
NdeII	GATC	5 cut(s)	71, 76, 464, 793, 1293
NlaIII	CATG	9 cut(s)	508, 605, 793, 813, 871, 886, 899, 1132, 1234
NlaIV	GGNNCC	7 cut(s)	288, 795, 1028, 1106, 1107, 1177, 1236
NmuCI	GTSAC	2 cut(s)	235, 247
NsiI	ATGCAT	3 cut(s)	506, 736, 901
NspI	RCATGY	3 cut(s)	508, 605, 886
NspV	TTCGAA	1 cut(s)	1055
PaeI	GCATGC	1 cut(s)	508
PctI	GAATGC	2 cut(s)	734, 1372
PfeI	GAWTC	4 cut(s)	50, 379, 568, 1112
PfoI	TCCNGGA	2 cut(s)	542, 1015
PkrI	GCNGC	9 cut(s)	274, 370, 627, 703, 844, 923, 1084, 1269, 1371
PleI	GAGTC	6 cut(s)	133, 315, 505, 867, 1327, 1442
PpsI	GAGTC	6 cut(s)	133, 315, 505, 867, 1327, 1442
PpuMI	RGGWCCY	1 cut(s)	1105
Psp5II	RGGWCCY	1 cut(s)	1105
Psp6I	CCWGG	4 cut(s)	402, 542, 1015, 1262
PspFI	CCCAGC	2 cut(s)	1276, 1345
PspGI	CCWGG	4 cut(s)	402, 542, 1015, 1262
PspN4I	GGNNCC	7 cut(s)	288, 795, 1028, 1106, 1107, 1177, 1236
PspPI	GGNCC	3 cut(s)	1026, 1105, 1379
PspPPI	RGGWCCY	1 cut(s)	1105
PstI	CTGCAG	2 cut(s)	277, 1272
PstNI	CAGNNNCTG	4 cut(s)	266, 281, 577, 1115
PsuI	RGATCY	2 cut(s)	464, 793
RsaI	GTAC	2 cut(s)	113, 1236
RsaNI	GTAC	2 cut(s)	112, 1235
RseI	CAYNNNNRTG	1 cut(s)	213
SaqAI	TTAA	6 cut(s)	188, 428, 686, 942, 1073, 1154
SatI	GCNGC	9 cut(s)	273, 369, 626, 702, 843, 922, 1083, 1268, 1370
Sau3AI	GATC	5 cut(s)	71, 76, 464, 793, 1293
Sau96I	GGNCC	3 cut(s)	1026, 1105, 1379
SchI	GAGTC	6 cut(s)	134, 315, 505, 868, 1328, 1443
ScrFI	CCNGG	5 cut(s)	404, 544, 847, 1017, 1264
SduI	GDGCHC	4 cut(s)	47, 210, 724, 1277
SfaNI	GCATC	4 cut(s)	208, 491, 908, 1302
SfcI	CTRYAG	2 cut(s)	273, 1268
SfuI	TTCGAA	1 cut(s)	1055
SinI	GGWCC	3 cut(s)	1026, 1105, 1379
SmiMI	CAYNNNNRTG	1 cut(s)	213
SmlI	CTYRAG	2 cut(s)	297, 614
SmoI	CTYRAG	2 cut(s)	297, 614
SphI	GCATGC	1 cut(s)	508
Sse9I	AATT	5 cut(s)	185, 939, 970, 1070, 1404
SsiI	CCGC	6 cut(s)	258, 625, 921, 1248, 1356, 1369
SspMI	CTAG	2 cut(s)	1044, 1202
StyD4I	CCNGG	5 cut(s)	402, 542, 845, 1015, 1262
StyI	CCWWGG	4 cut(s)	851, 1091, 1364, 1385
TaaI	ACNGT	3 cut(s)	144, 247, 657
TaiI	ACGT	1 cut(s)	541
TaqI	TCGA	4 cut(s)	394, 1055, 1066, 1437
TaqII	GACCGA	1 cut(s)	1014
TasI	AATT	5 cut(s)	185, 939, 970, 1070, 1404
TauI	GCSGC	3 cut(s)	628, 924, 1372
TfiI	GAWTC	4 cut(s)	50, 379, 568, 1112
Tru1I	TTAA	6 cut(s)	188, 428, 686, 942, 1073, 1154
Tru9I	TTAA	6 cut(s)	188, 428, 686, 942, 1073, 1154
TscAI	CASTG	3 cut(s)	267, 1003, 1141
TseFI	GTSAC	2 cut(s)	235, 247
TseI	GCWGC	6 cut(s)	272, 368, 701, 842, 1082, 1267
Tsp45I	GTSAC	2 cut(s)	235, 247
TspDTI	ATGAA	2 cut(s)	651, 778
TspRI	CASTG	3 cut(s)	267, 1003, 1141
VpaK11BI	GGWCC	3 cut(s)	1026, 1105, 1379
XagI	CCTNNNNNAGG	1 cut(s)	1183
XceI	RCATGY	3 cut(s)	508, 605, 886
XmiI	GTMKAC	1 cut(s)	128
XspI	CTAG	2 cut(s)	1044, 1202
Zsp2I	ATGCAT	3 cut(s)	506, 736, 901

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.