Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014194

Physical Location & Seq

Genomic Coordinates Reverse (-)

1032099 .. 1034973

Gene Structure 2875 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G03860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGGAGCTCGAGACCTCAGTTTATCGACCAAACGTCGCCGTTTATGATTCTCCGGATGGTGTGGAGGTTCGAGGACGGTACGACCAAATCTTCGCCAAGATTTTGACGAGAGAAGCTCTTAGCTTTGTGGCTGAGTTACAGAGAGAGTTTCGTGGCCATGTGAAGTATGCCATGGAGTGCCGGAGAGAGGCAAGACGGCGTTATAACTCCGGTGCCGTTCCTGGGTTTGATCCTTCCACTAAGTTCATCAGAGACGGTGATTGGAGCTGTGCCTCCGTCCCTCCTGCCGTTGCTGACCGGAGAGTTGAGATCACCGGTCCGGTCGAGAGGAAGATGATCATCAACGCTCTTAACTCCGGAGCTAAAGTCTTTATGGCTGATTTCGAGGACGCTTTGTCTCCAAGCTGGGAGAATCTGATGAGAGGGCATGTGAACCTGAAAGATGCAGTGGATGGATCCATTACGTTCCACGACAAGTCAAGGAACAGAGTTTACAAGCTCAACGACCAAACCGCTAAGCTTTTTGTCCGGCCAAGAGGTTGGCATCTCCCGGAGGCTCACATCCTCATCGACGGTGAACCCGCCACTGGATGTCTTGTCGATTTTGGCCTTTACTTCTTCCACAACTACGCCAAATTTAGACAGACTCAAGGTTCCGGTTTCGGTCCATTCTTCTATCTCCCCAAAATGGAACATTCTAGGGAAGCAAAGATATGGAACAGTGTTTTTGAGCGTGCTGAGAAGATGGCTGGGATAGAGAGAGGAAGCATTAGAGCAACTGTGCTTATCGAGACCCTCCCGGCTGTCTTCCAGATGAATGAGATACTCTATGAACTACGTGATCACTCAGTCGGTTTAAACTGTGGAAGATGGGATTATATCTTCAGCTATGTCAAAACCTTCCAAGCTCACCCTGACCGGCTCTTACCAGACCGTGTCCTAGTTGGCATGGGCCAGCATTTCATGAGAAGTTACTCTGATCTTCTCATCCGTACTTGTCATAAGCGCGGTGTCCACGCCATGGGTGGCATGGCGGCTCAGATTCCGATAAGAGATGACCCAAAAGCGAATGAGATGGCATTGGATCTAGTGAGGAAAGACAAGCTGAGAGAGGTAAGAGCAGGGCATGATGGAACATGGGCGGCTCATCCAGGACTCATCCCAATCTGCATGGAAGCATTCACTGGTCACATGGGAAAAAGTCCAAACCAAATCAAATCAGTGAAGCGCGAGGACGCAGCCGCAATAACAGAGGAAGATCTGCTTCAAATACCGCGAGGAGTGCGTACACTCGAGGGTTTAAGGCTGAACACACGTGTAGGAATTCAATACTTAGCCGCATGGTTAACAGGATCAGGATCTGTCCCACTCTATAACCTAATGGAAGATGCTGCTACGGCTGAGATAAGCCGTGTCCAGAATTGGCAATGGATTAGGTATGGAGTGGAACTTGATGGAGACGGGCTTGGAGTTCGAGTGAGTAAAGAGTTGTTTGGGAGAGTTGTCGAAGAAGAGATGGAAAGAATTGAGAAAGAAGTTGGGAAAGACAAGTTTAAGAATGGAATGTATAAGGAAGCTTGCAAGATGTTTACAAAGCAGTGTACTGCACCTGAGCTTGATGATTTTCTTACACTTGCTGTTTATAATCACATTGTAGCTCACTACCCTATCAATGTCTCAAGGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004474 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016740 GO:0016746 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00620 ko00630 ko01100 ko01110 ko01120 ko01200 map00620 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

Malate_synthase

Protein Analysis

562

Amino Acids

63.89

Weight (kDa)

8.02

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_N	PF20656	19 - 81	7.3e-23	Malate synthase, N-terminal domain
MS_TIM-barrel	PF01274	173 - 424	7.8e-121	Malate synthase, TIM barrel domain
MS_C	PF20659	429 - 551	8.2e-46	Malate synthase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03860 AT5G03860
fragaria_vesca	FvH4_5g07710
malus_domestica	MD06G1147700.v1.1
prunus_persica	Prupe.5G154600_v2.0.a1
pyrus_communis	pycom06g13710
rosa_chinensis	RchiOBHm_Chr7g0190721
rosa_laevigata	RLG00000004501
rosa_multiflora	Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264350
rosa_rugosa	Rorug06G0506700
rosa_samantha	Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana	Rw7G009890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	204, 1644
AccB1I	GGYRCC	1 cut(s)	212
AccB7I	CCANNNNNTGG	1 cut(s)	1021
AccII	CGCG	3 cut(s)	1008, 1230, 1276
AccIII	TCCGGA	2 cut(s)	52, 356
AciI	CCGC	8 cut(s)	513, 582, 1008, 1034, 1142, 1242, 1274, 1338
AclWI	GGATC	6 cut(s)	224, 450, 463, 1092, 1360, 1366
AcoI	YGGCCR	2 cut(s)	154, 530
AcsI	RAATTY	2 cut(s)	635, 1323
AcuI	CTGAAG	1 cut(s)	868
AcvI	CACGTG	1 cut(s)	1316
AfaI	GTAC	4 cut(s)	80, 994, 1288, 1603
AfiI	CCNNNNNNNGG	5 cut(s)	187, 222, 587, 688, 1021
AflIII	ACRYGT	2 cut(s)	1313, 1315
AgeI	ACCGGT	1 cut(s)	314
AgsI	TTSAA	2 cut(s)	1268, 1328
AhdI	GACNNNNNGTC	2 cut(s)	32, 394
AjnI	CCWGG	2 cut(s)	220, 1150
Alw21I	GWGCWC	1 cut(s)	9
Alw26I	GTCTC	6 cut(s)	5, 246, 402, 785, 1452, 1681
AlwI	GGATC	6 cut(s)	224, 450, 463, 1092, 1360, 1366
AlwNI	CAGNNNCTG	1 cut(s)	1361
Ama87I	CYCGRG	2 cut(s)	8, 1292
Aor13HI	TCCGGA	2 cut(s)	52, 356
AoxI	GGCC	4 cut(s)	154, 530, 607, 952
ApeKI	GCWGC	2 cut(s)	1238, 1391
ApoI	RAATTY	2 cut(s)	635, 1323
ArsI	GACNNNNNNTTYG	2 cut(s)	74, 106
AsiGI	ACCGGT	1 cut(s)	314
AspLEI	GCGC	2 cut(s)	1008, 1230
AspS9I	GGNCC	3 cut(s)	317, 665, 952
AsuC2I	CCSGG	2 cut(s)	551, 800
AsuHPI	GGTGA	4 cut(s)	269, 304, 587, 902
AvaI	CYCGRG	2 cut(s)	8, 1292
AvaII	GGWCC	2 cut(s)	317, 665
BalI	TGGCCA	1 cut(s)	156
BamHI	GGATCC	1 cut(s)	455
BanI	GGYRCC	1 cut(s)	212
BanII	GRGCYC	1 cut(s)	9
BbrPI	CACGTG	1 cut(s)	1316
BbsI	GAAGAC	1 cut(s)	799
Bbv12I	GWGCWC	1 cut(s)	9
BbvI	GCAGC	2 cut(s)	1250, 1378
BccI	CCATC	8 cut(s)	50, 446, 739, 864, 1069, 1124, 1448, 1510
BceAI	ACGGC	6 cut(s)	23, 200, 212, 272, 1395, 1413
BciT130I	CCWGG	2 cut(s)	222, 1152
BclI	TGATCA	2 cut(s)	336, 841
BcnI	CCSGG	2 cut(s)	551, 800
BcoDI	GTCTC	6 cut(s)	5, 246, 402, 785, 1452, 1681
BfaI	CTAG	3 cut(s)	699, 941, 1088
BglII	AGATCT	1 cut(s)	1258
BisI	GCNGC	6 cut(s)	1035, 1143, 1239, 1242, 1338, 1392
BlpI	GCTNAGC	1 cut(s)	516
BlsI	GCNGC	6 cut(s)	1036, 1144, 1240, 1243, 1339, 1393
Bme1390I	CCNGG	4 cut(s)	222, 551, 800, 1152
Bme18I	GGWCC	2 cut(s)	317, 665
BmeRI	GACNNNNNGTC	2 cut(s)	32, 394
BmeT110I	CYCGRG	2 cut(s)	8, 1292
BmgT120I	GGNCC	3 cut(s)	317, 665, 952
BmiI	GGNNCC	3 cut(s)	214, 457, 655
BmrFI	CCNGG	4 cut(s)	222, 551, 800, 1152
BmsI	GCATC	3 cut(s)	433, 553, 1378
BpiI	GAAGAC	1 cut(s)	799
BplI	GAGNNNNNCTC	2 cut(s)	100, 132
Bpu10I	CCTNAGC	1 cut(s)	1611
Bpu1102I	GCTNAGC	1 cut(s)	516
BpuEI	CTTGAG	2 cut(s)	633, 1663
BpuMI	CCSGG	2 cut(s)	551, 800
BsaAI	YACGTR	2 cut(s)	839, 1316
BsaBI	GATNNNNATC	2 cut(s)	338, 1357
BsaI	GGTCTC	2 cut(s)	5, 785
BsaJI	CCNNGG	3 cut(s)	171, 221, 1020
BsaWI	WCCGGW	7 cut(s)	52, 209, 297, 314, 319, 356, 656
BsaXI	ACNNNNNCTCC	4 cut(s)	351, 381, 1461, 1491
Bsc4I	CCNNNNNNNGG	5 cut(s)	187, 222, 587, 688, 1021
Bse118I	RCCGGY	2 cut(s)	314, 918
Bse1I	ACTGG	2 cut(s)	592, 1189
Bse3DI	GCAATG	1 cut(s)	1433
Bse8I	GATNNNNATC	2 cut(s)	338, 1357
BseAI	TCCGGA	2 cut(s)	52, 356
BseBI	CCWGG	2 cut(s)	222, 1152
BseDI	CCNNGG	3 cut(s)	171, 221, 1020
BseGI	GGATG	7 cut(s)	61, 457, 561, 596, 987, 1147, 1158
BseJI	GATNNNNATC	2 cut(s)	338, 1357
BseLI	CCNNNNNNNGG	5 cut(s)	187, 222, 587, 688, 1021
BseMI	GCAATG	1 cut(s)	1433
BseMII	CTCAG	8 cut(s)	30, 123, 729, 861, 1052, 1097, 1392, 1602
BseNI	ACTGG	2 cut(s)	592, 1189
BseRI	GAGGAG	1 cut(s)	1293
BseXI	GCAGC	2 cut(s)	1250, 1378
BseYI	CCCAGC	2 cut(s)	405, 749
BsgI	GTGCAG	1 cut(s)	1590
Bsh1236I	CGCG	3 cut(s)	1008, 1230, 1276
Bsh1285I	CGRYCG	1 cut(s)	324
BshFI	GGCC	4 cut(s)	156, 532, 609, 954
BshNI	GGYRCC	1 cut(s)	212
BshTI	ACCGGT	1 cut(s)	314
BsiEI	CGRYCG	1 cut(s)	324
BsiHKAI	GWGCWC	1 cut(s)	9
BsiHKCI	CYCGRG	2 cut(s)	8, 1292
BslFI	GGGAC	2 cut(s)	263, 1349
BslI	CCNNNNNNNGG	5 cut(s)	187, 222, 587, 688, 1021
BsmAI	GTCTC	6 cut(s)	5, 246, 402, 785, 1452, 1681
BsmBI	CGTCTC	2 cut(s)	246, 1452
BsmFI	GGGAC	2 cut(s)	263, 1349
BsmI	GAATGC	1 cut(s)	1178
BsnI	GGCC	4 cut(s)	156, 532, 609, 954
Bso31I	GGTCTC	2 cut(s)	5, 785
BsoBI	CYCGRG	2 cut(s)	8, 1292
Bsp1286I	GDGCHC	1 cut(s)	9
Bsp13I	TCCGGA	2 cut(s)	52, 356
Bsp1720I	GCTNAGC	1 cut(s)	516
Bsp19I	CCATGG	2 cut(s)	171, 1020
BspACI	CCGC	8 cut(s)	513, 582, 1008, 1034, 1142, 1242, 1274, 1338
BspANI	GGCC	4 cut(s)	156, 532, 609, 954
BspCNI	CTCAG	8 cut(s)	29, 124, 730, 860, 1051, 1098, 1393, 1603
BspEI	TCCGGA	2 cut(s)	52, 356
BspFNI	CGCG	3 cut(s)	1008, 1230, 1276
BspHI	TCATGA	1 cut(s)	963
BspLI	GGNNCC	3 cut(s)	214, 457, 655
BspPI	GGATC	6 cut(s)	224, 450, 463, 1092, 1360, 1366
BspT107I	GGYRCC	1 cut(s)	212
BspTNI	GGTCTC	2 cut(s)	5, 785
BsrDI	GCAATG	1 cut(s)	1433
BsrFI	RCCGGY	2 cut(s)	314, 918
BsrI	ACTGG	2 cut(s)	592, 1189
BssAI	RCCGGY	2 cut(s)	314, 918
BssECI	CCNNGG	3 cut(s)	171, 221, 1020
BssT1I	CCWWGG	2 cut(s)	171, 1020
Bst2UI	CCWGG	2 cut(s)	222, 1152
Bst4CI	ACNGT	7 cut(s)	78, 257, 575, 722, 781, 863, 935
Bst6I	CTCTTC	1 cut(s)	1506
BstBAI	YACGTR	2 cut(s)	839, 1316
BstC8I	GCNNGC	3 cut(s)	735, 956, 1579
BstDSI	CCRYGG	2 cut(s)	171, 1020
BstF5I	GGATG	7 cut(s)	61, 457, 561, 596, 987, 1147, 1158
BstFNI	CGCG	3 cut(s)	1008, 1230, 1276
BstHHI	GCGC	2 cut(s)	1008, 1230
BstMAI	GTCTC	6 cut(s)	5, 246, 402, 785, 1452, 1681
BstMCI	CGRYCG	1 cut(s)	324
BstMWI	GCNNNNNNNGC	2 cut(s)	1282, 1397
BstNI	CCWGG	2 cut(s)	222, 1152
BstNSI	RCATGY	1 cut(s)	431
BstSCI	CCNGG	4 cut(s)	220, 549, 798, 1150
BstUI	CGCG	3 cut(s)	1008, 1230, 1276
BstV1I	GCAGC	2 cut(s)	1250, 1378
BstV2I	GAAGAC	1 cut(s)	799
BstX2I	RGATCY	4 cut(s)	455, 1084, 1258, 1358
BstXI	CCANNNNNNTGG	1 cut(s)	540
BstYI	RGATCY	4 cut(s)	455, 1084, 1258, 1358
BsuRI	GGCC	4 cut(s)	156, 532, 609, 954
BtgI	CCRYGG	2 cut(s)	171, 1020
BtsCI	GGATG	7 cut(s)	61, 457, 561, 596, 987, 1147, 1158
BtsI	GCAGTG	2 cut(s)	453, 1604
BtsIMutI	CAGTG	6 cut(s)	453, 585, 727, 1182, 1227, 1604
Cac8I	GCNNGC	3 cut(s)	735, 956, 1579
CaiI	CAGNNNCTG	1 cut(s)	1361
CciI	TCATGA	1 cut(s)	963
CfoI	GCGC	2 cut(s)	1008, 1230
Cfr10I	RCCGGY	2 cut(s)	314, 918
Cfr13I	GGNCC	3 cut(s)	317, 665, 952
CpoI	CGGWCCG	1 cut(s)	317
CseI	GACGC	2 cut(s)	398, 1244
Csp6I	GTAC	4 cut(s)	79, 993, 1287, 1602
CspAI	ACCGGT	1 cut(s)	314
CspI	CGGWCCG	1 cut(s)	317
CviQI	GTAC	4 cut(s)	79, 993, 1287, 1602
DraI	TTTAAA	1 cut(s)	858
DriI	GACNNNNNGTC	2 cut(s)	32, 394
EaeI	YGGCCR	2 cut(s)	154, 530
Eam1104I	CTCTTC	1 cut(s)	1506
Eam1105I	GACNNNNNGTC	2 cut(s)	32, 394
EarI	CTCTTC	1 cut(s)	1506
Ecl136II	GAGCTC	1 cut(s)	7
Eco130I	CCWWGG	2 cut(s)	171, 1020
Eco24I	GRGCYC	1 cut(s)	9
Eco31I	GGTCTC	2 cut(s)	5, 785
Eco47I	GGWCC	2 cut(s)	317, 665
Eco53kI	GAGCTC	1 cut(s)	7
Eco57I	CTGAAG	1 cut(s)	868
Eco72I	CACGTG	1 cut(s)	1316
Eco88I	CYCGRG	2 cut(s)	8, 1292
EcoICRI	GAGCTC	1 cut(s)	7
EcoRI	GAATTC	1 cut(s)	1323
EcoRII	CCWGG	2 cut(s)	220, 1150
EcoT14I	CCWWGG	2 cut(s)	171, 1020
EcoT38I	GRGCYC	1 cut(s)	9
ErhI	CCWWGG	2 cut(s)	171, 1020
Esp3I	CGTCTC	2 cut(s)	246, 1452
FalI	AAGNNNNNCTT	2 cut(s)	1248, 1280
FaqI	GGGAC	2 cut(s)	263, 1349
FauI	CCCGC	1 cut(s)	589
FbaI	TGATCA	2 cut(s)	336, 841
Fnu4HI	GCNGC	6 cut(s)	1035, 1143, 1239, 1242, 1338, 1392
FokI	GGATG	7 cut(s)	68, 464, 548, 603, 974, 1134, 1145
FriOI	GRGCYC	1 cut(s)	9
Fsp4HI	GCNGC	6 cut(s)	1035, 1143, 1239, 1242, 1338, 1392
FspBI	CTAG	3 cut(s)	699, 941, 1088
GlaI	GCGC	2 cut(s)	1007, 1229
GluI	GCNGC	6 cut(s)	1035, 1143, 1239, 1242, 1338, 1392
GsaI	CCCAGC	2 cut(s)	409, 753
HaeIII	GGCC	4 cut(s)	156, 532, 609, 954
HgaI	GACGC	2 cut(s)	398, 1244
HhaI	GCGC	2 cut(s)	1008, 1230
Hin6I	GCGC	2 cut(s)	1006, 1228
HinP1I	GCGC	2 cut(s)	1006, 1228
HincII	GTYRAC	1 cut(s)	1347
HindII	GTYRAC	1 cut(s)	1347
HindIII	AAGCTT	2 cut(s)	518, 1575
HinfI	GANTC	5 cut(s)	47, 412, 646, 1042, 1155
HpaI	GTTAAC	1 cut(s)	1347
HphI	GGTGA	4 cut(s)	269, 304, 587, 902
Hpy166II	GTNNAC	8 cut(s)	433, 493, 578, 1015, 1289, 1347, 1590, 1602
Hpy188I	TCNGA	6 cut(s)	251, 417, 979, 1041, 1047, 1688
Hpy188III	TCNNGA	9 cut(s)	10, 53, 325, 357, 790, 811, 964, 1356, 1417
Hpy8I	GTNNAC	8 cut(s)	433, 493, 578, 1015, 1289, 1347, 1590, 1602
Hpy99I	CGWCG	2 cut(s)	38, 575
HpyAV	CCTTC	2 cut(s)	243, 910
HpyCH4III	ACNGT	7 cut(s)	78, 257, 575, 722, 781, 863, 935
HpyCH4IV	ACGT	4 cut(s)	33, 464, 838, 1315
HpyCH4V	TGCA	4 cut(s)	446, 1170, 1581, 1607
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1282, 1397
HpySE526I	ACGT	4 cut(s)	33, 464, 838, 1315
HspAI	GCGC	2 cut(s)	1006, 1228
Kpn2I	TCCGGA	2 cut(s)	52, 356
Ksp22I	TGATCA	2 cut(s)	336, 841
KspAI	GTTAAC	1 cut(s)	1347
LmnI	GCTCC	3 cut(s)	4, 264, 359
Lsp1109I	GCAGC	2 cut(s)	1250, 1378
LweI	GCATC	3 cut(s)	433, 553, 1378
MaeI	CTAG	3 cut(s)	699, 941, 1088
MaeII	ACGT	4 cut(s)	33, 464, 838, 1315
MaeIII	GTNAC	3 cut(s)	135, 971, 1187
MflI	RGATCY	4 cut(s)	455, 1084, 1258, 1358
MhlI	GDGCHC	1 cut(s)	9
MlsI	TGGCCA	1 cut(s)	156
MluCI	AATT	4 cut(s)	635, 1323, 1420, 1524
MluNI	TGGCCA	1 cut(s)	156
MlyI	GAGTC	2 cut(s)	640, 1149
Mox20I	TGGCCA	1 cut(s)	156
MroI	TCCGGA	2 cut(s)	52, 356
MscI	TGGCCA	1 cut(s)	156
MseI	TTAA	5 cut(s)	351, 857, 1301, 1346, 1554
Msp20I	TGGCCA	1 cut(s)	156
MspR9I	CCNGG	4 cut(s)	222, 551, 800, 1152
MssI	GTTTAAAC	1 cut(s)	858
Mva1269I	GAATGC	1 cut(s)	1178
MvaI	CCWGG	2 cut(s)	222, 1152
MvnI	CGCG	3 cut(s)	1008, 1230, 1276
MwoI	GCNNNNNNNGC	2 cut(s)	1282, 1397
NciI	CCSGG	2 cut(s)	551, 800
NcoI	CCATGG	2 cut(s)	171, 1020
NlaIV	GGNNCC	3 cut(s)	214, 457, 655
NmuCI	GTSAC	1 cut(s)	1187
NspI	RCATGY	1 cut(s)	431
PaeR7I	CTCGAG	2 cut(s)	8, 1292
PagI	TCATGA	1 cut(s)	963
PctI	GAATGC	1 cut(s)	1178
PfeI	GAWTC	3 cut(s)	47, 412, 1042
PflFI	GACNNNGTC	1 cut(s)	935
PflMI	CCANNNNNTGG	1 cut(s)	1021
PfoI	TCCNGGA	2 cut(s)	549, 1150
PinAI	ACCGGT	1 cut(s)	314
PkrI	GCNGC	6 cut(s)	1036, 1144, 1240, 1243, 1339, 1393
PleI	GAGTC	2 cut(s)	640, 1149
PmaCI	CACGTG	1 cut(s)	1316
PmeI	GTTTAAAC	1 cut(s)	858
PmlI	CACGTG	1 cut(s)	1316
PpsI	GAGTC	2 cut(s)	640, 1149
Ppu21I	YACGTR	2 cut(s)	839, 1316
PsiI	TTATAA	2 cut(s)	204, 1644
Psp124BI	GAGCTC	1 cut(s)	9
Psp6I	CCWGG	2 cut(s)	220, 1150
PspCI	CACGTG	1 cut(s)	1316
PspFI	CCCAGC	2 cut(s)	405, 749
PspGI	CCWGG	2 cut(s)	220, 1150
PspN4I	GGNNCC	3 cut(s)	214, 457, 655
PspPI	GGNCC	3 cut(s)	317, 665, 952
PspXI	VCTCGAGB	1 cut(s)	1292
PsrI	GAACNNNNNNTAC	2 cut(s)	476, 508
PstNI	CAGNNNCTG	1 cut(s)	1361
PsuI	RGATCY	4 cut(s)	455, 1084, 1258, 1358
PsyI	GACNNNGTC	1 cut(s)	935
RsaI	GTAC	4 cut(s)	80, 994, 1288, 1603
RsaNI	GTAC	4 cut(s)	79, 993, 1287, 1602
Rsr2I	CGGWCCG	1 cut(s)	317
RsrII	CGGWCCG	1 cut(s)	317
SacI	GAGCTC	1 cut(s)	9
SaqAI	TTAA	5 cut(s)	351, 857, 1301, 1346, 1554
SatI	GCNGC	6 cut(s)	1035, 1143, 1239, 1242, 1338, 1392
Sau96I	GGNCC	3 cut(s)	317, 665, 952
SchI	GAGTC	2 cut(s)	640, 1149
ScrFI	CCNGG	4 cut(s)	222, 551, 800, 1152
SduI	GDGCHC	1 cut(s)	9
SfaNI	GCATC	3 cut(s)	433, 553, 1378
Sfr274I	CTCGAG	2 cut(s)	8, 1292
SinI	GGWCC	2 cut(s)	317, 665
SlaI	CTCGAG	2 cut(s)	8, 1292
SmlI	CTYRAG	4 cut(s)	8, 648, 1292, 1678
SmoI	CTYRAG	4 cut(s)	8, 648, 1292, 1678
Sse9I	AATT	4 cut(s)	635, 1323, 1420, 1524
SsiI	CCGC	8 cut(s)	513, 582, 1008, 1034, 1142, 1242, 1274, 1338
SspMI	CTAG	3 cut(s)	699, 941, 1088
SstI	GAGCTC	1 cut(s)	9
StyD4I	CCNGG	4 cut(s)	220, 549, 798, 1150
StyI	CCWWGG	2 cut(s)	171, 1020
TaaI	ACNGT	7 cut(s)	78, 257, 575, 722, 781, 863, 935
TaiI	ACGT	4 cut(s)	36, 467, 841, 1318
TaqII	GACCGA	1 cut(s)	653
TasI	AATT	4 cut(s)	635, 1323, 1420, 1524
TatI	WGTACW	1 cut(s)	1601
TauI	GCSGC	4 cut(s)	1037, 1145, 1244, 1340
TfiI	GAWTC	3 cut(s)	47, 412, 1042
Tru1I	TTAA	5 cut(s)	351, 857, 1301, 1346, 1554
Tru9I	TTAA	5 cut(s)	351, 857, 1301, 1346, 1554
TscAI	CASTG	6 cut(s)	453, 592, 727, 1189, 1227, 1604
TseFI	GTSAC	1 cut(s)	1187
TseI	GCWGC	2 cut(s)	1238, 1391
Tsp45I	GTSAC	1 cut(s)	1187
TspDTI	ATGAA	4 cut(s)	235, 830, 846, 952
TspGWI	ACGGA	2 cut(s)	265, 980
TspRI	CASTG	6 cut(s)	453, 592, 727, 1189, 1227, 1604
Tth111I	GACNNNGTC	1 cut(s)	935
Van91I	CCANNNNNTGG	1 cut(s)	1021
VpaK11BI	GGWCC	2 cut(s)	317, 665
XapI	RAATTY	2 cut(s)	635, 1323
XceI	RCATGY	1 cut(s)	431
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1022, 1027
XhoI	CTCGAG	2 cut(s)	8, 1292
XspI	CTAG	3 cut(s)	699, 941, 1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.