FvH4_5g07710

Belongs to the malate synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014194
Physical Location & Seq
Reverse (-)
4516058 .. 4519179
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07710.t1

Sequence Viewer

Length: 1704 bp
ATGATGGGAGTTGGCTCTTACGGGTACGCTGCACCGGCCGGTGCAGGAGGGGGATACGACGCCCCCGAAGGAGTTGAGATTCGGGGACGGTATGACCAAGAGTATGCGAAGATTCTGAGCAAGGAGGCTTTGCAGTTCGTGGCGGAGTTGCAGAGGGAGTTCAGGAACAGGATCAAGTATGCATTGGAGTGTAGGAAGGAAGCTAAGAGGAGGTACAATGAGGGCGGTGTGCCAGGGTTTGATCCCGCCACGAAGTTCGTAAGGGAAGGGGATTGGGTTTGTGCACCGGTCCCGAAAGCGGTGGCTGATCGGAGAGTGGAGATTACAGGACCTGTGGAGAGGAAGATGGTCATCAATGCTCTCAATTCTGGAGCAAAAGTTTTCATGGCTGATTTTGAAGATGCGCTTTCACCAAGCTGGGAGAACCTTATAAGGGGTCAAGTGAACCTGAAAGATGCTGTGGATGGGAGCATAACTTTCCATGATAAGGCGAGGAACAGAGTCTACAAGCTCAATGATTGCAACATAGCCAAGCTTTTTGTGCGTCCAAGAGGTTGGCACTTGCCTGAGGCTCATATTTTCATTGATGGTGAGCCTGCAACTGGTTGCCTTGTGGACTTTGGTCTCTACTTCTACCACAACTATGCAGCTTTCAGTCGAACCCAAGGTGAAGGGTCTGGACCTTTCTTCTACCTCCCTAAAATGGAGCATTCAAGGGAAGCGAAGATATGGAATTGTGTGTTCGAGAAGGCCGAGAAGATGGTAGGGATAGAGAGAGGAAGCATCAGAGCCACTGTTCTGATCGAAACTCTTCCGGCTGTGTTTCAAATGGATGAAATCCTCTATGAGCTTAGGGATCACTCTGTTGGTTTGAACTGTGGAAGATGGGATTACATTTTTAGCTATGTCAAGACCTTCCAGGCTCACCCCGACCGCCTATTGCCTGATCGGGTTCTGGTTGGCATGAATCAACATTTCATGAGGAGTTACTCTGATCTACTCATCAGGACCTGCCATAGGCGCGGCGTCCACGCCATGGGAGGCATGGCAGCTCAGATACCAATTAGAGATGATCCAGTAGCAAATGAAGCAGCATTGGATTTGGTGAGGAAGGACAAGCTAAGAGAAGTGAAAGCAGGACATGATGGAACCTGGGCAGCACATCCAGGACTAATCCCAGCCTGCATGGAGATCTTCACAAACAACATGGGCCACACCCCTAACCAAATCCAAACCATGAGGCGAGAAGATGCATCCGCCATAACTGAAGAAGACCTCTTGCAGAGGCCCAGAGGGGTCCGAACCCTGGAGGGTCTGCGGCTGAACACCCGAGTAGGAATCCAATACCTGGCAGCATGGCTAACAGGAGCTGGTTCGGTACCTCTTTACAATCTCATGGAAGATGCTGCAACAGCTGAGATCAGCAGGGTCCAGAACTGGCAGTGGATCAAGTATGGAGTGGAGTTGGATGGAGATGGACTTGGAGTGAGGGTGGGGAAGGAGCTGTTTGGGAGGGTGGTGGAAGAAGAAATGGAGAGGATTGAGAGAGAGATTGGGAAGGACAAGTTTAAGAAGGGAATGTACAAAGAGGCTTGCAAGATGTTCACAAGGCAATGCACAGCGCAAACTCTAGACGACTTTCTGACTCTCGATGCCTATAATCATATCGTCTTGCACCACCCCAGGGCATCATCCAGGCTCTAG

Protein Analysis

568

Amino Acids

64.16

Weight (kDa)

7.25

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_N PF20656 23 - 85 3.7e-21 Malate synthase, N-terminal domain
MS_TIM-barrel PF01274 178 - 428 6.6e-118 Malate synthase, TIM barrel domain
MS_C PF20659 434 - 556 2.4e-44 Malate synthase, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03860 AT5G03860
fragaria_vesca FvH4_5g07710
malus_domestica MD06G1147700.v1.1
prunus_persica Prupe.5G154600_v2.0.a1
pyrus_communis pycom06g13710
rosa_chinensis RchiOBHm_Chr7g0190721
rosa_laevigata RLG00000004501
rosa_multiflora Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii Rroxscaffold_3G00264350
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana Rw7G009890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 1019
Acc65I GGTACC 1 cut(s) 1378
AccB1I GGYRCC 1 cut(s) 1378
AccB7I CCANNNNNTGG 2 cut(s) 1036, 1348
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 1023
AciI CCGC 8 cut(s) 143, 225, 246, 299, 934, 1023, 1257, 1318
AclWI GGATC 5 cut(s) 179, 236, 864, 1067, 1454
AcoI YGGCCR 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 1287
AcyI GRCGYC 2 cut(s) 60, 1026
AfaI GTAC 4 cut(s) 26, 215, 1380, 1583
AgeI ACCGGT 1 cut(s) 286
AgsI TTSAA 4 cut(s) 398, 714, 827, 874
AjnI CCWGG 8 cut(s) 232, 918, 1151, 1165, 1305, 1347, 1682, 1694
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 1 cut(s) 629
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 5 cut(s) 179, 236, 864, 1067, 1454
AlwNI CAGNNNCTG 3 cut(s) 332, 1011, 1370
Ama87I CYCGRG 1 cut(s) 1329
AoxI GGCC 4 cut(s) 36, 750, 1210, 1286
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 7 cut(s) 29, 647, 1049, 1091, 1157, 1352, 1406
AsiGI ACCGGT 1 cut(s) 286
Asp700I GAANNNNTTC 1 cut(s) 810
Asp718I GGTACC 1 cut(s) 1378
AspLEI GCGC 3 cut(s) 406, 1023, 1624
AspS9I GGNCC 8 cut(s) 289, 329, 680, 1008, 1210, 1287, 1297, 1429
AsuHPI GGTGA 5 cut(s) 402, 602, 680, 917, 1117
AvaI CYCGRG 1 cut(s) 1329
AvaII GGWCC 6 cut(s) 289, 329, 680, 1008, 1297, 1429
AxyI CCTNAGG 1 cut(s) 567
BaeGI GKGCMC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 1378
BarI GAAGNNNNNNTAC 2 cut(s) 1565, 1597
BbsI GAAGAC 1 cut(s) 1278
Bbv12I GWGCWC 1 cut(s) 286
BbvI GCAGC 7 cut(s) 16, 659, 1061, 1103, 1169, 1364, 1393
BccI CCATC 8 cut(s) 340, 458, 581, 754, 879, 1139, 1463, 1469
BciT130I CCWGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
BciVI GTATCC 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 629
BfaI CTAG 2 cut(s) 1631, 1702
BfuAI ACCTGC 1 cut(s) 1019
BfuI GTATCC 1 cut(s) 47
BglII AGATCT 1 cut(s) 1191
BisI GCNGC 9 cut(s) 30, 648, 1024, 1050, 1092, 1158, 1319, 1353, 1407
BlsI GCNGC 9 cut(s) 31, 649, 1025, 1051, 1093, 1159, 1320, 1354, 1408
Bme1390I CCNGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
Bme18I GGWCC 6 cut(s) 289, 329, 680, 1008, 1297, 1429
BmeT110I CYCGRG 1 cut(s) 1329
BmgT120I GGNCC 8 cut(s) 289, 329, 680, 1008, 1210, 1287, 1297, 1429
BmiI GGNNCC 5 cut(s) 291, 1150, 1298, 1380, 1430
BmrFI CCNGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
BmsI GCATC 8 cut(s) 391, 445, 792, 1240, 1262, 1393, 1642, 1697
BoxI GACNNNNGTC 1 cut(s) 621
BpiI GAAGAC 1 cut(s) 1278
BpmI CTGGAG 2 cut(s) 390, 1328
Bpu10I CCTNAGC 1 cut(s) 851
BsaBI GATNNNNATC 1 cut(s) 350
BsaHI GRCGYC 2 cut(s) 60, 1026
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 7 cut(s) 233, 664, 1035, 1152, 1305, 1682, 1683
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 4 cut(s) 363, 393, 1476, 1506
Bse118I RCCGGY 3 cut(s) 34, 38, 286
Bse1I ACTGG 3 cut(s) 607, 1076, 1442
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 1 cut(s) 1619
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
BseDI CCNNGG 7 cut(s) 233, 664, 1035, 1152, 1305, 1682, 1683
BseGI GGATG 6 cut(s) 469, 838, 1162, 1253, 1474, 1691
BseJI GATNNNNATC 1 cut(s) 350
BseMI GCAATG 1 cut(s) 1619
BseMII CTCAG 4 cut(s) 107, 558, 1067, 1407
BseNI ACTGG 3 cut(s) 607, 1076, 1442
BseRI GAGGAG 2 cut(s) 223, 997
BseSI GKGCMC 1 cut(s) 286
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 7 cut(s) 16, 659, 1061, 1103, 1169, 1364, 1393
BseYI CCCAGC 2 cut(s) 417, 1177
BsgI GTGCAG 2 cut(s) 15, 63
Bsh1236I CGCG 1 cut(s) 1023
Bsh1285I CGRYCG 2 cut(s) 39, 934
BshFI GGCC 4 cut(s) 38, 752, 1212, 1288
BshNI GGYRCC 1 cut(s) 1378
BshTI ACCGGT 1 cut(s) 286
BsiEI CGRYCG 2 cut(s) 39, 934
BsiHKAI GWGCWC 1 cut(s) 286
BsiHKCI CYCGRG 1 cut(s) 1329
BsiSI CCGG 4 cut(s) 35, 39, 287, 815
BslFI GGGAC 2 cut(s) 99, 275
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 2 cut(s) 99, 275
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 4 cut(s) 38, 752, 1212, 1288
Bso31I GGTCTC 1 cut(s) 629
BsoBI CYCGRG 1 cut(s) 1329
Bsp1286I GDGCHC 1 cut(s) 286
Bsp1407I TGTACA 1 cut(s) 1581
Bsp19I CCATGG 1 cut(s) 1035
BspACI CCGC 8 cut(s) 143, 225, 246, 299, 934, 1023, 1257, 1318
BspANI GGCC 4 cut(s) 38, 752, 1212, 1288
BspCNI CTCAG 4 cut(s) 108, 559, 1066, 1408
BspFNI CGCG 1 cut(s) 1023
BspHI TCATGA 1 cut(s) 978
BspLI GGNNCC 5 cut(s) 291, 1150, 1298, 1380, 1430
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 5 cut(s) 179, 236, 864, 1067, 1454
BspT107I GGYRCC 1 cut(s) 1378
BspTNI GGTCTC 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 1619
BsrFI RCCGGY 3 cut(s) 34, 38, 286
BsrGI TGTACA 1 cut(s) 1581
BsrI ACTGG 3 cut(s) 607, 1076, 1442
BssAI RCCGGY 3 cut(s) 34, 38, 286
BssECI CCNNGG 7 cut(s) 233, 664, 1035, 1152, 1305, 1682, 1683
BssNI GRCGYC 2 cut(s) 60, 1026
BssT1I CCWWGG 2 cut(s) 664, 1035
Bst2UI CCWGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
Bst4CI ACNGT 3 cut(s) 90, 796, 878
Bst6I CTCTTC 1 cut(s) 816
BstACI GRCGYC 2 cut(s) 60, 1026
BstAUI TGTACA 1 cut(s) 1581
BstC8I GCNNGC 3 cut(s) 597, 1183, 1594
BstDEI CTNAG 7 cut(s) 116, 204, 567, 851, 1053, 1121, 1416
BstDSI CCRYGG 1 cut(s) 1035
BstENI CCTNNNNNAGG 1 cut(s) 1015
BstF5I GGATG 6 cut(s) 469, 838, 1162, 1253, 1474, 1691
BstFNI CGCG 1 cut(s) 1023
BstHHI GCGC 3 cut(s) 406, 1023, 1624
BstMAI GTCTC 1 cut(s) 629
BstMCI CGRYCG 2 cut(s) 39, 934
BstMWI GCNNNNNNNGC 5 cut(s) 35, 541, 1020, 1088, 1412
BstNI CCWGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
BstPAI GACNNNNGTC 1 cut(s) 621
BstSCI CCNGG 8 cut(s) 232, 918, 1151, 1165, 1305, 1347, 1682, 1694
BstSLI GKGCMC 1 cut(s) 286
BstUI CGCG 1 cut(s) 1023
BstV1I GCAGC 7 cut(s) 16, 659, 1061, 1103, 1169, 1364, 1393
BstV2I GAAGAC 1 cut(s) 1278
BstX2I RGATCY 1 cut(s) 1191
BstXI CCANNNNNNTGG 1 cut(s) 555
BstYI RGATCY 1 cut(s) 1191
BstZI CGGCCG 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 567
BsuI GTATCC 1 cut(s) 47
BsuRI GGCC 4 cut(s) 38, 752, 1212, 1288
BtgI CCRYGG 1 cut(s) 1035
BtsCI GGATG 6 cut(s) 469, 838, 1162, 1253, 1474, 1691
BtsI GCAGTG 1 cut(s) 1448
BtsIMutI CAGTG 2 cut(s) 792, 1448
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 3 cut(s) 597, 1183, 1594
CaiI CAGNNNCTG 3 cut(s) 332, 1011, 1370
CciI TCATGA 1 cut(s) 978
CfoI GCGC 3 cut(s) 406, 1023, 1624
Cfr10I RCCGGY 3 cut(s) 34, 38, 286
Cfr13I GGNCC 8 cut(s) 289, 329, 680, 1008, 1210, 1287, 1297, 1429
CseI GACGC 3 cut(s) 68, 533, 1015
Csp6I GTAC 4 cut(s) 25, 214, 1379, 1582
CspAI ACCGGT 1 cut(s) 286
CviQI GTAC 4 cut(s) 25, 214, 1379, 1582
DdeI CTNAG 7 cut(s) 116, 204, 567, 851, 1053, 1121, 1416
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
EciI GGCGGA 2 cut(s) 158, 1246
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 2 cut(s) 664, 1035
Eco31I GGTCTC 1 cut(s) 629
Eco47I GGWCC 6 cut(s) 289, 329, 680, 1008, 1297, 1429
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 1287
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 1329
EcoNI CCTNNNNNAGG 1 cut(s) 1015
EcoO109I RGGNCCY 2 cut(s) 329, 1008
EcoRII CCWGG 8 cut(s) 232, 918, 1151, 1165, 1305, 1347, 1682, 1694
EcoT14I CCWWGG 2 cut(s) 664, 1035
EcoT22I ATGCAT 2 cut(s) 184, 1255
ErhI CCWWGG 2 cut(s) 664, 1035
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 2 cut(s) 99, 275
FauI CCCGC 1 cut(s) 253
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 9 cut(s) 30, 648, 1024, 1050, 1092, 1158, 1319, 1353, 1407
FokI GGATG 6 cut(s) 476, 845, 1149, 1240, 1481, 1678
Fsp4HI GCNGC 9 cut(s) 30, 648, 1024, 1050, 1092, 1158, 1319, 1353, 1407
FspBI CTAG 2 cut(s) 1631, 1702
GlaI GCGC 3 cut(s) 405, 1022, 1623
GluI GCNGC 9 cut(s) 30, 648, 1024, 1050, 1092, 1158, 1319, 1353, 1407
GsaI CCCAGC 2 cut(s) 421, 1181
GsuI CTGGAG 2 cut(s) 390, 1328
HaeIII GGCC 4 cut(s) 38, 752, 1212, 1288
HapII CCGG 4 cut(s) 35, 39, 287, 815
HgaI GACGC 3 cut(s) 68, 533, 1015
HhaI GCGC 3 cut(s) 406, 1023, 1624
Hin1I GRCGYC 2 cut(s) 60, 1026
Hin6I GCGC 3 cut(s) 404, 1021, 1622
HinP1I GCGC 3 cut(s) 404, 1021, 1622
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 6 cut(s) 79, 112, 501, 967, 1338, 1645
HpaII CCGG 4 cut(s) 35, 39, 287, 815
HphI GGTGA 5 cut(s) 402, 602, 680, 917, 1117
Hpy166II GTNNAC 6 cut(s) 284, 445, 505, 616, 1030, 1605
Hpy188I TCNGA 8 cut(s) 117, 312, 788, 801, 994, 1056, 1301, 1644
Hpy8I GTNNAC 6 cut(s) 284, 445, 505, 616, 1030, 1605
Hpy99I CGWCG 1 cut(s) 62
HpyCH4III ACNGT 3 cut(s) 90, 796, 878
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 541, 1020, 1088, 1412
HpyF3I CTNAG 7 cut(s) 116, 204, 567, 851, 1053, 1121, 1416
Hsp92I GRCGYC 2 cut(s) 60, 1026
HspAI GCGC 3 cut(s) 404, 1021, 1622
KpnI GGTACC 1 cut(s) 1382
LmnI GCTCC 5 cut(s) 371, 468, 706, 1367, 1501
Lsp1109I GCAGC 7 cut(s) 16, 659, 1061, 1103, 1169, 1364, 1393
LweI GCATC 8 cut(s) 391, 445, 792, 1240, 1262, 1393, 1642, 1697
MaeI CTAG 2 cut(s) 1631, 1702
MaeIII GTNAC 1 cut(s) 986
MflI RGATCY 1 cut(s) 1191
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 3 cut(s) 364, 733, 1062
MlyI GAGTC 2 cut(s) 510, 1639
MmeI TCCRAC 1 cut(s) 1446
Mph1103I ATGCAT 2 cut(s) 184, 1255
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 1 cut(s) 1569
MslI CAYNNNNRTG 2 cut(s) 187, 642
MspA1I CMGCKG 1 cut(s) 1415
MspI CCGG 4 cut(s) 35, 39, 287, 815
MspR9I CCNGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
MvnI CGCG 1 cut(s) 1023
MwoI GCNNNNNNNGC 5 cut(s) 35, 541, 1020, 1088, 1412
NcoI CCATGG 1 cut(s) 1035
NlaIV GGNNCC 5 cut(s) 291, 1150, 1298, 1380, 1430
NmeAIII GCCGAG 1 cut(s) 778
NsiI ATGCAT 2 cut(s) 184, 1255
PagI TCATGA 1 cut(s) 978
PasI CCCWGGG 1 cut(s) 1683
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 750
PctI GAATGC 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 4 cut(s) 79, 112, 967, 1338
PflMI CCANNNNNTGG 2 cut(s) 1036, 1348
PfoI TCCNGGA 1 cut(s) 1165
PinAI ACCGGT 1 cut(s) 286
PkrI GCNGC 9 cut(s) 31, 649, 1025, 1051, 1093, 1159, 1320, 1354, 1408
PleI GAGTC 2 cut(s) 509, 1639
PpsI GAGTC 2 cut(s) 509, 1639
PpuMI RGGWCCY 2 cut(s) 329, 1008
PshAI GACNNNNGTC 1 cut(s) 621
PsiI TTATAA 1 cut(s) 431
Psp5II RGGWCCY 2 cut(s) 329, 1008
Psp6I CCWGG 8 cut(s) 232, 918, 1151, 1165, 1305, 1347, 1682, 1694
PspFI CCCAGC 2 cut(s) 417, 1177
PspGI CCWGG 8 cut(s) 232, 918, 1151, 1165, 1305, 1347, 1682, 1694
PspN4I GGNNCC 5 cut(s) 291, 1150, 1298, 1380, 1430
PspPI GGNCC 8 cut(s) 289, 329, 680, 1008, 1210, 1287, 1297, 1429
PspPPI RGGWCCY 2 cut(s) 329, 1008
PsrI GAACNNNNNNTAC 2 cut(s) 488, 520
PstNI CAGNNNCTG 3 cut(s) 332, 1011, 1370
PsuI RGATCY 1 cut(s) 1191
PvuII CAGCTG 1 cut(s) 1415
RsaI GTAC 4 cut(s) 26, 215, 1380, 1583
RsaNI GTAC 4 cut(s) 25, 214, 1379, 1582
RseI CAYNNNNRTG 2 cut(s) 187, 642
SaqAI TTAA 1 cut(s) 1569
SatI GCNGC 9 cut(s) 30, 648, 1024, 1050, 1092, 1158, 1319, 1353, 1407
Sau96I GGNCC 8 cut(s) 289, 329, 680, 1008, 1210, 1287, 1297, 1429
SchI GAGTC 2 cut(s) 510, 1639
ScrFI CCNGG 8 cut(s) 234, 920, 1153, 1167, 1307, 1349, 1684, 1696
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 8 cut(s) 391, 445, 792, 1240, 1262, 1393, 1642, 1697
SinI GGWCC 6 cut(s) 289, 329, 680, 1008, 1297, 1429
SmiMI CAYNNNNRTG 2 cut(s) 187, 642
Sse9I AATT 3 cut(s) 364, 733, 1062
SsiI CCGC 8 cut(s) 143, 225, 246, 299, 934, 1023, 1257, 1318
SspMI CTAG 2 cut(s) 1631, 1702
StyD4I CCNGG 8 cut(s) 232, 918, 1151, 1165, 1305, 1347, 1682, 1694
StyI CCWWGG 2 cut(s) 664, 1035
TaaI ACNGT 3 cut(s) 90, 796, 878
TaqI TCGA 4 cut(s) 658, 744, 804, 1650
TasI AATT 3 cut(s) 364, 733, 1062
TatI WGTACW 1 cut(s) 1581
TauI GCSGC 2 cut(s) 1026, 1321
TfiI GAWTC 4 cut(s) 79, 112, 967, 1338
Tru1I TTAA 1 cut(s) 1569
Tru9I TTAA 1 cut(s) 1569
TscAI CASTG 2 cut(s) 799, 1448
TseI GCWGC 7 cut(s) 29, 647, 1049, 1091, 1157, 1352, 1406
TspDTI ATGAA 6 cut(s) 373, 571, 849, 967, 980, 1101
TspRI CASTG 2 cut(s) 799, 1448
Van91I CCANNNNNTGG 2 cut(s) 1036, 1348
VneI GTGCAC 1 cut(s) 282
VpaK11BI GGWCC 6 cut(s) 289, 329, 680, 1008, 1297, 1429
XagI CCTNNNNNAGG 1 cut(s) 1015
XbaI TCTAGA 1 cut(s) 1630
XcmI CCANNNNNNNNNTGG 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 2 cut(s) 1631, 1702
Zsp2I ATGCAT 2 cut(s) 184, 1255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.