Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0014194

Physical Location & Seq

Genomic Coordinates Reverse (-)

59899100 .. 59901745

Gene Structure 2646 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004501

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGATGGGAGTTGGCTCGTACGGGTACACTGCACCAGCGACGAACATGAACACCGGTGCAAGAGGGGGCTACGATGCCCCCGATGGAGTTGAAATTCGGGGACGGTATGACCAAGAGTATGCGAAGATCCTGAGCAAGGAGGCTTTGCAGTTCGTGGTGGAGTTGCAGAGGGAGTTCAGGAACAGGATTAAGTATGCATTGGAGTGTAGGAAGGAAGCTAAGAGGAGGTACAATGAGGGTGGTCTACCCGGGTTTGACCCCGCCACGAAGTTCATAAGGGAAGGGGATTGGGTTTGTGCGCCGGTCCCGAAAGCGGTGGCTGATCGGAGGGTGGAGATTACAGGACCTGTGGAGAGGAAGATGGTCATCAATGCTCTCAATTCTGGAGCAAAAGTTTTCATGGCTGATTTTGAAGATGCACTTTCACCAAGCTGGGAGAATCTAATAAGAGGCCAAGTGAACCTGAAAGATGCTGTGGATGGGAGCATAACTTTCCATGATAAGGCCAGAAACAGAGTCTACAAGCTCAACGATCACAACATAGCCAAGCTTTTTGTGCGTCCAAGAGGTTGGCACTTACCTGAGACTCATATTCTCATTGATGGTGAGCCTGCAACTGGTTGCCTTGTCGACTTTGGTCTCTACTTCTACCATAACTATGCAGCATTCCGCCGCACCCAAGGTGAAGGGTCTGGACCTTTCTTCTACCTCCCTAAAATGGAGCATTCAAGGGAAGCGAAGATATGGAATTGTGTGTTCGAGAAGGCAGAGAAGATGGTAGGGATAGAGAGAGGAAGCATCAGAGCCACTGTTCTGATCGAAACACTTCCAGCTGTGTTTCAAATGGATGAAATCCTGTATGAGCTTAGGGATCACTCTGTTGGTTTGAACTGTGGAAGATGGGATTACATTTTCAGCTATGTCAAGACCTTCCAGGCTCACCCTGACCGCCTATTGCCTGACCGGGTTCTAGTTGGCATGAATCAACATTTCATGAGGAGTTACTCTGATCTCCTCATCAGGACCTGTCATAGGCGCGGTGTCCACGCCATGGGAGGCATGGCAGCTCAGATTCCAATTAGAGATGATCCAGTAGCAAATGAAGCAGCATTGGATTTGGTGAGGAAGGACAAGCTGAGAGAAGTGAAAGCAGGACATGATGGAACTTGGGCAGCACATCCAGGACTAATCCCAGCCTGCATGGAAATCTTCACAAACAACATGGGCCACAACACCCCCAACCAAATCCAAACCATGAGGCGAGAAGATGCATCCTCCATAACCGAGGAAGACCTCTTGCAGAGGCCTAGAGGAGTCCGAACCCTGGAGGGTCTCCGGCTGAACACCCGGGTGGGAATCCAGTACCTGGCAGCATGGCTAACAGGAGCTGGTTCGGTGCCACTTTACAATCTCATGGAAGATGCTGCAACAGCTGAGATAAGCAGGGTTCAGAACTGGCAGTGGATCAAGTATGGAGTGGAGTTGGATGGAGATGGACTTGGAGTGAGAGTGGGGAAGGAGCTGTTTGGAAGAGTGGTGGAAGACGAAATGGAGAGGATTGAAAGAGAGGTTGGGAAGGACAAATTTAAGAAGGGAATGTACAAAGAGGCTTGCAAGATGTTCACAAGGCAATGCACAGCGCAAACTCTAGACGATTTTCTGACTCTCGATGCTTATAATCATATCGTCTTGCACCACCCCAAGGCATCATCCAGGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004474 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016740 GO:0016746 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00620 ko00630 ko01100 ko01110 ko01120 ko01200 map00620 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

Malate_synthase

Protein Analysis

574

Amino Acids

65.07

Weight (kDa)

7.98

Isoelectric Point (pI)

41.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_N	PF20656	28 - 90	5.8e-21	Malate synthase, N-terminal domain
MS_TIM-barrel	PF01274	183 - 434	4.1e-117	Malate synthase, TIM barrel domain
MS_C	PF20659	440 - 562	4.5e-44	Malate synthase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03860 AT5G03860
fragaria_vesca	FvH4_5g07710
malus_domestica	MD06G1147700.v1.1
prunus_persica	Prupe.5G154600_v2.0.a1
pyrus_communis	pycom06g13710
rosa_chinensis	RchiOBHm_Chr7g0190721
rosa_laevigata	RLG00000004501
rosa_multiflora	Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264350
rosa_rugosa	Rorug06G0506700
rosa_samantha	Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana	Rw7G009890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1677
AccB1I	GGYRCC	1 cut(s)	1396
AccB7I	CCANNNNNTGG	2 cut(s)	1051, 1366
AccI	GTMKAC	3 cut(s)	244, 519, 630
AccII	CGCG	1 cut(s)	1038
AciI	CCGC	6 cut(s)	261, 314, 670, 673, 949, 1038
AclWI	GGATC	4 cut(s)	121, 879, 1082, 1472
AcsI	RAATTY	2 cut(s)	93, 1583
AfaI	GTAC	5 cut(s)	20, 26, 230, 1364, 1601
AgeI	ACCGGT	1 cut(s)	53
AgsI	TTSAA	6 cut(s)	92, 413, 729, 842, 889, 1562
AjnI	CCWGG	5 cut(s)	933, 1180, 1323, 1365, 1712
AjuI	GAANNNNNNNTTGG	2 cut(s)	1554, 1586
AleI	CACNNNNGTG	1 cut(s)	1349
Alw26I	GTCTC	3 cut(s)	578, 644, 1337
AlwI	GGATC	4 cut(s)	121, 879, 1082, 1472
AlwNI	CAGNNNCTG	4 cut(s)	347, 1026, 1366, 1388
Ama87I	CYCGRG	2 cut(s)	248, 1347
AoxI	GGCC	4 cut(s)	451, 504, 1225, 1304
ApeKI	GCWGC	6 cut(s)	662, 1064, 1106, 1172, 1370, 1424
ApoI	RAATTY	2 cut(s)	93, 1583
AsiGI	ACCGGT	1 cut(s)	53
Asp700I	GAANNNNTTC	1 cut(s)	825
AspLEI	GCGC	3 cut(s)	301, 1038, 1642
AspS9I	GGNCC	5 cut(s)	304, 344, 695, 1023, 1225
AsuC2I	CCSGG	5 cut(s)	249, 250, 965, 1348, 1349
AsuHPI	GGTGA	5 cut(s)	417, 617, 695, 932, 1132
AvaI	CYCGRG	2 cut(s)	248, 1347
AvaII	GGWCC	4 cut(s)	304, 344, 695, 1023
BanI	GGYRCC	1 cut(s)	1396
BarI	GAAGNNNNNNTAC	2 cut(s)	1583, 1615
BbsI	GAAGAC	2 cut(s)	1296, 1548
BbvI	GCAGC	6 cut(s)	674, 1076, 1118, 1184, 1382, 1411
BccI	CCATC	9 cut(s)	77, 355, 473, 596, 769, 894, 1154, 1481, 1487
BciT130I	CCWGG	5 cut(s)	935, 1182, 1325, 1367, 1714
BcnI	CCSGG	5 cut(s)	249, 250, 965, 1348, 1349
BcoDI	GTCTC	3 cut(s)	578, 644, 1337
BfaI	CTAG	3 cut(s)	971, 1308, 1649
BisI	GCNGC	7 cut(s)	663, 673, 1065, 1107, 1173, 1371, 1425
BlsI	GCNGC	7 cut(s)	664, 674, 1066, 1108, 1174, 1372, 1426
Bme18I	GGWCC	4 cut(s)	304, 344, 695, 1023
BmeT110I	CYCGRG	2 cut(s)	248, 1347
BmgT120I	GGNCC	5 cut(s)	304, 344, 695, 1023, 1225
BmiI	GGNNCC	2 cut(s)	306, 1398
BmsI	GCATC	9 cut(s)	64, 406, 460, 807, 1258, 1280, 1411, 1660, 1715
BoxI	GACNNNNGTC	1 cut(s)	636
BpiI	GAAGAC	2 cut(s)	1296, 1548
BpmI	CTGGAG	2 cut(s)	405, 1346
Bpu10I	CCTNAGC	2 cut(s)	131, 866
BpuMI	CCSGG	5 cut(s)	249, 250, 965, 1348, 1349
BsaBI	GATNNNNATC	1 cut(s)	365
BsaI	GGTCTC	2 cut(s)	644, 1337
BsaJI	CCNNGG	7 cut(s)	248, 679, 1050, 1284, 1323, 1347, 1701
BsaWI	WCCGGW	1 cut(s)	53
BsaXI	ACNNNNNCTCC	4 cut(s)	378, 408, 1494, 1524
Bse118I	RCCGGY	2 cut(s)	53, 301
Bse1I	ACTGG	4 cut(s)	622, 1091, 1360, 1460
Bse3DI	GCAATG	1 cut(s)	1637
Bse8I	GATNNNNATC	1 cut(s)	365
BseBI	CCWGG	5 cut(s)	935, 1182, 1325, 1367, 1714
BseDI	CCNNGG	7 cut(s)	248, 679, 1050, 1284, 1323, 1347, 1701
BseGI	GGATG	6 cut(s)	484, 853, 1177, 1271, 1492, 1709
BseJI	GATNNNNATC	1 cut(s)	365
BseMI	GCAATG	1 cut(s)	1637
BseMII	CTCAG	5 cut(s)	122, 573, 1082, 1127, 1425
BseNI	ACTGG	4 cut(s)	622, 1091, 1360, 1460
BseRI	GAGGAG	4 cut(s)	238, 1004, 1012, 1326
BseXI	GCAGC	6 cut(s)	674, 1076, 1118, 1184, 1382, 1411
BseYI	CCCAGC	2 cut(s)	432, 1192
BsgI	GTGCAG	1 cut(s)	15
Bsh1236I	CGCG	1 cut(s)	1038
BshFI	GGCC	4 cut(s)	453, 506, 1227, 1306
BshNI	GGYRCC	1 cut(s)	1396
BshTI	ACCGGT	1 cut(s)	53
BsiHKCI	CYCGRG	2 cut(s)	248, 1347
BsiSI	CCGG	6 cut(s)	54, 249, 302, 964, 1336, 1348
BsiWI	CGTACG	1 cut(s)	18
BslFI	GGGAC	2 cut(s)	114, 290
BsmAI	GTCTC	3 cut(s)	578, 644, 1337
BsmFI	GGGAC	2 cut(s)	114, 290
BsmI	GAATGC	2 cut(s)	665, 724
BsnI	GGCC	4 cut(s)	453, 506, 1227, 1306
Bso31I	GGTCTC	2 cut(s)	644, 1337
BsoBI	CYCGRG	2 cut(s)	248, 1347
Bsp1407I	TGTACA	1 cut(s)	1599
Bsp143I	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
Bsp19I	CCATGG	1 cut(s)	1050
BspACI	CCGC	6 cut(s)	261, 314, 670, 673, 949, 1038
BspANI	GGCC	4 cut(s)	453, 506, 1227, 1306
BspCNI	CTCAG	5 cut(s)	123, 574, 1081, 1128, 1426
BspFNI	CGCG	1 cut(s)	1038
BspHI	TCATGA	1 cut(s)	993
BspLI	GGNNCC	2 cut(s)	306, 1398
BspPI	GGATC	4 cut(s)	121, 879, 1082, 1472
BspT107I	GGYRCC	1 cut(s)	1396
BspTNI	GGTCTC	2 cut(s)	644, 1337
BsrDI	GCAATG	1 cut(s)	1637
BsrFI	RCCGGY	2 cut(s)	53, 301
BsrGI	TGTACA	1 cut(s)	1599
BsrI	ACTGG	4 cut(s)	622, 1091, 1360, 1460
BssAI	RCCGGY	2 cut(s)	53, 301
BssECI	CCNNGG	7 cut(s)	248, 679, 1050, 1284, 1323, 1347, 1701
BssMI	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
BssT1I	CCWWGG	3 cut(s)	679, 1050, 1701
Bst2UI	CCWGG	5 cut(s)	935, 1182, 1325, 1367, 1714
Bst4CI	ACNGT	3 cut(s)	105, 811, 893
Bst6I	CTCTTC	1 cut(s)	1525
BstAUI	TGTACA	1 cut(s)	1599
BstC8I	GCNNGC	3 cut(s)	612, 1198, 1612
BstDEI	CTNAG	7 cut(s)	131, 219, 582, 866, 1068, 1136, 1434
BstDSI	CCRYGG	1 cut(s)	1050
BstENI	CCTNNNNNAGG	2 cut(s)	134, 1030
BstF5I	GGATG	6 cut(s)	484, 853, 1177, 1271, 1492, 1709
BstFNI	CGCG	1 cut(s)	1038
BstHHI	GCGC	3 cut(s)	301, 1038, 1642
BstKTI	GATC	8 cut(s)	129, 325, 535, 819, 874, 1012, 1090, 1467
BstMAI	GTCTC	3 cut(s)	578, 644, 1337
BstMBI	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
BstMWI	GCNNNNNNNGC	3 cut(s)	556, 1103, 1430
BstNI	CCWGG	5 cut(s)	935, 1182, 1325, 1367, 1714
BstPAI	GACNNNNGTC	1 cut(s)	636
BstUI	CGCG	1 cut(s)	1038
BstV1I	GCAGC	6 cut(s)	674, 1076, 1118, 1184, 1382, 1411
BstV2I	GAAGAC	2 cut(s)	1296, 1548
BstX2I	RGATCY	1 cut(s)	126
BstXI	CCANNNNNNTGG	1 cut(s)	570
BstYI	RGATCY	1 cut(s)	126
BsuRI	GGCC	4 cut(s)	453, 506, 1227, 1306
BtgI	CCRYGG	1 cut(s)	1050
BtsCI	GGATG	6 cut(s)	484, 853, 1177, 1271, 1492, 1709
BtsI	GCAGTG	2 cut(s)	27, 1466
BtsIMutI	CAGTG	3 cut(s)	27, 807, 1466
Cac8I	GCNNGC	3 cut(s)	612, 1198, 1612
CaiI	CAGNNNCTG	4 cut(s)	347, 1026, 1366, 1388
CciI	TCATGA	1 cut(s)	993
CfoI	GCGC	3 cut(s)	301, 1038, 1642
Cfr10I	RCCGGY	2 cut(s)	53, 301
Cfr13I	GGNCC	5 cut(s)	304, 344, 695, 1023, 1225
Cfr9I	CCCGGG	2 cut(s)	248, 1347
CseI	GACGC	1 cut(s)	548
Csp6I	GTAC	5 cut(s)	19, 25, 229, 1363, 1600
CspAI	ACCGGT	1 cut(s)	53
CspCI	CAANNNNNGTGG	2 cut(s)	220, 255
CviQI	GTAC	5 cut(s)	19, 25, 229, 1363, 1600
DdeI	CTNAG	7 cut(s)	131, 219, 582, 866, 1068, 1136, 1434
DpnI	GATC	8 cut(s)	128, 324, 534, 818, 873, 1011, 1089, 1466
DpnII	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
Eam1104I	CTCTTC	1 cut(s)	1525
EarI	CTCTTC	1 cut(s)	1525
EciI	GGCGGA	1 cut(s)	659
Eco130I	CCWWGG	3 cut(s)	679, 1050, 1701
Eco147I	AGGCCT	1 cut(s)	1306
Eco31I	GGTCTC	2 cut(s)	644, 1337
Eco47I	GGWCC	4 cut(s)	304, 344, 695, 1023
Eco88I	CYCGRG	2 cut(s)	248, 1347
EcoNI	CCTNNNNNAGG	2 cut(s)	134, 1030
EcoO109I	RGGNCCY	2 cut(s)	344, 1023
EcoRII	CCWGG	5 cut(s)	933, 1180, 1323, 1365, 1712
EcoT14I	CCWWGG	3 cut(s)	679, 1050, 1701
EcoT22I	ATGCAT	2 cut(s)	199, 1273
ErhI	CCWWGG	3 cut(s)	679, 1050, 1701
FalI	AAGNNNNNCTT	2 cut(s)	405, 437
FaqI	GGGAC	2 cut(s)	114, 290
FauI	CCCGC	1 cut(s)	268
FblI	GTMKAC	3 cut(s)	244, 519, 630
Fnu4HI	GCNGC	7 cut(s)	663, 673, 1065, 1107, 1173, 1371, 1425
FokI	GGATG	6 cut(s)	491, 860, 1164, 1258, 1499, 1696
Fsp4HI	GCNGC	7 cut(s)	663, 673, 1065, 1107, 1173, 1371, 1425
FspBI	CTAG	3 cut(s)	971, 1308, 1649
GlaI	GCGC	3 cut(s)	300, 1037, 1641
GluI	GCNGC	7 cut(s)	663, 673, 1065, 1107, 1173, 1371, 1425
GsaI	CCCAGC	2 cut(s)	436, 1196
GsuI	CTGGAG	2 cut(s)	405, 1346
HaeIII	GGCC	4 cut(s)	453, 506, 1227, 1306
HapII	CCGG	6 cut(s)	54, 249, 302, 964, 1336, 1348
HgaI	GACGC	1 cut(s)	548
HhaI	GCGC	3 cut(s)	301, 1038, 1642
Hin6I	GCGC	3 cut(s)	299, 1036, 1640
HinP1I	GCGC	3 cut(s)	299, 1036, 1640
HincII	GTYRAC	1 cut(s)	631
HindII	GTYRAC	1 cut(s)	631
HindIII	AAGCTT	1 cut(s)	548
HinfI	GANTC	8 cut(s)	439, 516, 586, 982, 1072, 1314, 1356, 1663
HpaII	CCGG	6 cut(s)	54, 249, 302, 964, 1336, 1348
HphI	GGTGA	5 cut(s)	417, 617, 695, 932, 1132
Hpy166II	GTNNAC	7 cut(s)	27, 245, 460, 520, 631, 1045, 1623
Hpy188I	TCNGA	8 cut(s)	327, 803, 816, 1009, 1071, 1319, 1452, 1662
Hpy8I	GTNNAC	7 cut(s)	27, 245, 460, 520, 631, 1045, 1623
Hpy99I	CGWCG	1 cut(s)	43
HpyAV	CCTTC	9 cut(s)	205, 275, 680, 757, 940, 1120, 1510, 1570, 1585
HpyCH4III	ACNGT	3 cut(s)	105, 811, 893
HpyF10VI	GCNNNNNNNGC	3 cut(s)	556, 1103, 1430
HpyF3I	CTNAG	7 cut(s)	131, 219, 582, 866, 1068, 1136, 1434
HspAI	GCGC	3 cut(s)	299, 1036, 1640
Kzo9I	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
LmnI	GCTCC	5 cut(s)	386, 483, 721, 1385, 1519
Lsp1109I	GCAGC	6 cut(s)	674, 1076, 1118, 1184, 1382, 1411
LweI	GCATC	9 cut(s)	64, 406, 460, 807, 1258, 1280, 1411, 1660, 1715
MaeI	CTAG	3 cut(s)	971, 1308, 1649
MaeIII	GTNAC	1 cut(s)	1001
MalI	GATC	8 cut(s)	128, 324, 534, 818, 873, 1011, 1089, 1466
MboI	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
MflI	RGATCY	1 cut(s)	126
MluCI	AATT	5 cut(s)	93, 379, 748, 1077, 1583
MlyI	GAGTC	4 cut(s)	525, 580, 1323, 1657
MmeI	TCCRAC	1 cut(s)	1464
Mph1103I	ATGCAT	2 cut(s)	199, 1273
MroXI	GAANNNNTTC	1 cut(s)	825
MseI	TTAA	2 cut(s)	189, 1587
MslI	CAYNNNNRTG	3 cut(s)	202, 657, 1349
MspA1I	CMGCKG	2 cut(s)	833, 1433
MspI	CCGG	6 cut(s)	54, 249, 302, 964, 1336, 1348
Mva1269I	GAATGC	2 cut(s)	665, 724
MvaI	CCWGG	5 cut(s)	935, 1182, 1325, 1367, 1714
MvnI	CGCG	1 cut(s)	1038
MwoI	GCNNNNNNNGC	3 cut(s)	556, 1103, 1430
NciI	CCSGG	5 cut(s)	249, 250, 965, 1348, 1349
NcoI	CCATGG	1 cut(s)	1050
NdeII	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
NlaIV	GGNNCC	2 cut(s)	306, 1398
NsiI	ATGCAT	2 cut(s)	199, 1273
OliI	CACNNNNGTG	1 cut(s)	1349
PagI	TCATGA	1 cut(s)	993
PceI	AGGCCT	1 cut(s)	1306
PcsI	WCGNNNNNNNCGW	1 cut(s)	78
PctI	GAATGC	2 cut(s)	665, 724
PdmI	GAANNNNTTC	1 cut(s)	825
PfeI	GAWTC	4 cut(s)	439, 982, 1072, 1356
Pfl23II	CGTACG	1 cut(s)	18
PflMI	CCANNNNNTGG	2 cut(s)	1051, 1366
PfoI	TCCNGGA	1 cut(s)	1180
PinAI	ACCGGT	1 cut(s)	53
PkrI	GCNGC	7 cut(s)	664, 674, 1066, 1108, 1174, 1372, 1426
PleI	GAGTC	4 cut(s)	524, 580, 1322, 1657
PpsI	GAGTC	4 cut(s)	524, 580, 1322, 1657
PpuMI	RGGWCCY	2 cut(s)	344, 1023
PshAI	GACNNNNGTC	1 cut(s)	636
PsiI	TTATAA	1 cut(s)	1677
Psp5II	RGGWCCY	2 cut(s)	344, 1023
Psp6I	CCWGG	5 cut(s)	933, 1180, 1323, 1365, 1712
PspFI	CCCAGC	2 cut(s)	432, 1192
PspGI	CCWGG	5 cut(s)	933, 1180, 1323, 1365, 1712
PspLI	CGTACG	1 cut(s)	18
PspN4I	GGNNCC	2 cut(s)	306, 1398
PspPI	GGNCC	5 cut(s)	304, 344, 695, 1023, 1225
PspPPI	RGGWCCY	2 cut(s)	344, 1023
PstNI	CAGNNNCTG	4 cut(s)	347, 1026, 1366, 1388
PsuI	RGATCY	1 cut(s)	126
PvuII	CAGCTG	2 cut(s)	833, 1433
RsaI	GTAC	5 cut(s)	20, 26, 230, 1364, 1601
RsaNI	GTAC	5 cut(s)	19, 25, 229, 1363, 1600
RseI	CAYNNNNRTG	3 cut(s)	202, 657, 1349
SalI	GTCGAC	1 cut(s)	629
SaqAI	TTAA	2 cut(s)	189, 1587
SatI	GCNGC	7 cut(s)	663, 673, 1065, 1107, 1173, 1371, 1425
Sau3AI	GATC	8 cut(s)	126, 322, 532, 816, 871, 1009, 1087, 1464
Sau96I	GGNCC	5 cut(s)	304, 344, 695, 1023, 1225
SchI	GAGTC	4 cut(s)	525, 580, 1323, 1657
SfaNI	GCATC	9 cut(s)	64, 406, 460, 807, 1258, 1280, 1411, 1660, 1715
SgrAI	CRCCGGYG	1 cut(s)	53
SinI	GGWCC	4 cut(s)	304, 344, 695, 1023
SmaI	CCCGGG	2 cut(s)	250, 1349
SmiMI	CAYNNNNRTG	3 cut(s)	202, 657, 1349
Sse9I	AATT	5 cut(s)	93, 379, 748, 1077, 1583
SseBI	AGGCCT	1 cut(s)	1306
SsiI	CCGC	6 cut(s)	261, 314, 670, 673, 949, 1038
SspMI	CTAG	3 cut(s)	971, 1308, 1649
StuI	AGGCCT	1 cut(s)	1306
StyI	CCWWGG	3 cut(s)	679, 1050, 1701
TaaI	ACNGT	3 cut(s)	105, 811, 893
TaqI	TCGA	4 cut(s)	630, 759, 819, 1668
TasI	AATT	5 cut(s)	93, 379, 748, 1077, 1583
TatI	WGTACW	1 cut(s)	1599
TauI	GCSGC	1 cut(s)	675
TfiI	GAWTC	4 cut(s)	439, 982, 1072, 1356
Tru1I	TTAA	2 cut(s)	189, 1587
Tru9I	TTAA	2 cut(s)	189, 1587
TscAI	CASTG	3 cut(s)	34, 814, 1466
TseI	GCWGC	6 cut(s)	662, 1064, 1106, 1172, 1370, 1424
TspDTI	ATGAA	7 cut(s)	62, 262, 388, 864, 982, 995, 1116
TspMI	CCCGGG	2 cut(s)	248, 1347
TspRI	CASTG	3 cut(s)	34, 814, 1466
Van91I	CCANNNNNTGG	2 cut(s)	1051, 1366
VpaK11BI	GGWCC	4 cut(s)	304, 344, 695, 1023
XagI	CCTNNNNNAGG	2 cut(s)	134, 1030
XapI	RAATTY	2 cut(s)	93, 1583
XbaI	TCTAGA	1 cut(s)	1648
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1057
XmaI	CCCGGG	2 cut(s)	248, 1347
XmiI	GTMKAC	3 cut(s)	244, 519, 630
XmnI	GAANNNNTTC	1 cut(s)	825
XspI	CTAG	3 cut(s)	971, 1308, 1649
Zsp2I	ATGCAT	2 cut(s)	199, 1273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.