Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0014194

Physical Location & Seq

Genomic Coordinates Forward (+)

28972560 .. 28976385

Gene Structure 3826 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1147700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGATAGGAGTTGGCACATATGGCTATCCTGAACAGGCCACCATGAGGAGCGGTGGTGGCGGCGGAGGATATGACGTTCCCGATGGTGTCGACATTCGGGGACGGTACGACGGAGAGTTTGCGAGGATTCTGACCAAGGATGCTTTACAGTTTGTGGTGGAGTTGCAGAGGGAATTTAGGAACCGAATCAAGTATGCGTTGGAGTGTCGGAAGGAGGCTAAGAGGAGGTACAATGAAGGGGCTTTGCCAGGGTTTGATCCTGCTACCAAGTTCATTAGGGAGGGGAATTGGGTTTGTGCTCCGGTACCGGAAGCGGTGGCGGATCGGAAGGTGGAGATTACGGGACCTGTGGAGAGGAAGATGATCATCAATGCTCTCAACTCTGGAGCCAAAGTTTTCATGGCGGACTTCGAAGATGCACTGTCACCAACTTGGGAGAACTTGATGAGAGGGCAAGTGAATCTGAAGGATGCTGTGGATGGGAGCATAACATTCCATGACAAGGCCAGGAATAGGGTTTACAAGCTGAACGACAGAAACACAGCCAAGCTTTTTGTTCGTCCGAGAGGTTGGCACTTGCCTGAGGCTCACATTTTCATTGATGGTGAGCCTGCAACTGGTTGCCTTGTGGACTTCGGCCTTTACTTTTACCACAACTATAAAGCATTCAGGCAAACCCAAGGGGCTGGATTTGGACCTTTCTTCTATCTCCCTAAAATGGAGACCTCAAGGGAAGCTAAGATATGGAACTGTGTGTTTGAGAGGGCAGAGAAGATGGCAGGGATAGAGAGAGGTAGCATCAGGGCAACTGTGCTGATTGAAACACTTCCAGCTGTGTTTCAAATGGATGAGATCCTCTATGAGCTGAGGGACCACTCTGTTGGCTTGAACTGTGGAAGATGGGATTACATTTTCAGCTATGTCAAAACCTTCCAGGCTCACCCCGACCGCCTCTTGCCAGACCGTGTTCTGGTGGGCATGACTCAACATTTCATGAGGAGCTACTCTGACCTCCTCATCCGGACGTGCCATAGGCGCGGCGTGCATGCCATGGGTGGCATGGCAGCTCAGATTCCAATCAGGGATGATCCAAAAGCAAATGAAGCAGCCTTGGACTTGGTGAGGAAAGACAAGCTAAGGGAGGTGAAAGCAGGACATGATGGAACATGGGCAGCTCACCCAGGGCTAATCCCAGCCTGCATGGATATCTTCACCAACAACATGCCGCCCAACACCCCTAACCAAATCCAATCCATGAGAAGAGAAGATGCATCCACCATAACAGAAGAAGACCTCCTCCAGAGGCCTCGCGGCGTCCGAACCCTAGAAGGTCTCCGGCTCAACACCCGGGTAGGGATCCAGTACGTGGCAGCATGGCTCACTGGGACAGGCTCAGTGCCACTCTACAACCTCATGGAGGATGCAGCCACTGCGGAAATAAGCAGGGTGCAGAACTGGCAGTGGATCAAGTATGGAGTGGAGTTGGACGGTGACGTGCTCGGAGTGAGGGTTGGGAAGGAGCTGTTTGGGAGAGTGGTGGACGAAGAAATGGAGAGGATTGAGAGGGAGGTTGGGAAGGAGAAGTTTAAGAAGGGAATGTATAAGGAGGCTTGCAGGATTTTCACCAGGCAATGCACCGCTCAAACACTTGATGATTTTCTCACTCTGGATGCCTACAATCGCATTGTGCTACACTACCCAAAGGGATCATCCAGGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004474 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016740 GO:0016746 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00620 ko00630 ko01100 ko01110 ko01120 ko01200 map00620 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

Malate_synthase

Protein Analysis

574

Amino Acids

65.12

Weight (kDa)

8.41

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_N	PF20656	28 - 90	1.5e-21	Malate synthase, N-terminal domain
MS_TIM-barrel	PF01274	183 - 434	8.4e-117	Malate synthase, TIM barrel domain
MS_C	PF20659	440 - 562	1e-44	Malate synthase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03860 AT5G03860
fragaria_vesca	FvH4_5g07710
malus_domestica	MD06G1147700.v1.1
prunus_persica	Prupe.5G154600_v2.0.a1
pyrus_communis	pycom06g13710
rosa_chinensis	RchiOBHm_Chr7g0190721
rosa_laevigata	RLG00000004501
rosa_multiflora	Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264350
rosa_rugosa	Rorug06G0506700
rosa_samantha	Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana	Rw7G009890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	304
AccB1I	GGYRCC	1 cut(s)	304
AccB7I	CCANNNNNTGG	1 cut(s)	1366
AccBSI	CCGCTC	2 cut(s)	51, 1640
AccI	GTMKAC	1 cut(s)	90
AccII	CGCG	2 cut(s)	1038, 1311
AccIII	TCCGGA	1 cut(s)	1020
AclWI	GGATC	8 cut(s)	251, 330, 847, 1082, 1351, 1364, 1472, 1714
AcsI	RAATTY	1 cut(s)	173
AcuI	CTGAAG	1 cut(s)	485
AcyI	GRCGYC	1 cut(s)	1314
AfaI	GTAC	4 cut(s)	107, 230, 306, 1364
AfiI	CCNNNNNNNGG	9 cut(s)	45, 502, 513, 617, 718, 970, 1353, 1366, 1417
AgsI	TTSAA	3 cut(s)	821, 842, 889
AjiI	CACGTC	2 cut(s)	1026, 1495
AjnI	CCWGG	6 cut(s)	247, 506, 933, 1180, 1625, 1712
AloI	GAACNNNNNNTCC	2 cut(s)	60, 92
Alw21I	GWGCWC	2 cut(s)	301, 1500
Alw26I	GTCTC	2 cut(s)	716, 1337
AlwI	GGATC	8 cut(s)	251, 330, 847, 1082, 1351, 1364, 1472, 1714
AlwNI	CAGNNNCTG	2 cut(s)	1430, 1719
Ama87I	CYCGRG	1 cut(s)	1347
Aor13HI	TCCGGA	1 cut(s)	1020
AoxI	GGCC	4 cut(s)	36, 504, 637, 1304
ApeKI	GCWGC	5 cut(s)	1064, 1106, 1172, 1370, 1424
ApoI	RAATTY	1 cut(s)	173
ArsI	GACNNNNNNTTYG	2 cut(s)	101, 133
Asp700I	GAANNNNTTC	1 cut(s)	825
Asp718I	GGTACC	1 cut(s)	304
AspLEI	GCGC	1 cut(s)	1038
AspS9I	GGNCC	3 cut(s)	344, 695, 871
AsuC2I	CCSGG	2 cut(s)	1348, 1349
AsuHPI	GGTGA	9 cut(s)	417, 617, 932, 1132, 1156, 1169, 1204, 1502, 1615
AsuII	TTCGAA	1 cut(s)	411
AvaI	CYCGRG	1 cut(s)	1347
AvaII	GGWCC	3 cut(s)	344, 695, 871
AxyI	CCTNAGG	1 cut(s)	582
BamHI	GGATCC	1 cut(s)	1356
BanI	GGYRCC	1 cut(s)	304
BbsI	GAAGAC	1 cut(s)	1296
Bbv12I	GWGCWC	2 cut(s)	301, 1500
BbvCI	CCTCAGC	1 cut(s)	866
BbvI	GCAGC	5 cut(s)	1076, 1118, 1184, 1382, 1436
BccI	CCATC	6 cut(s)	77, 473, 596, 769, 894, 1154
BciT130I	CCWGG	6 cut(s)	249, 508, 935, 1182, 1627, 1714
BclI	TGATCA	1 cut(s)	363
BcnI	CCSGG	2 cut(s)	1348, 1349
BcoDI	GTCTC	2 cut(s)	716, 1337
BfaI	CTAG	1 cut(s)	1325
BisI	GCNGC	9 cut(s)	61, 1039, 1065, 1107, 1173, 1226, 1312, 1371, 1425
BlsI	GCNGC	9 cut(s)	62, 1040, 1066, 1108, 1174, 1227, 1313, 1372, 1426
Bme1390I	CCNGG	8 cut(s)	249, 508, 935, 1182, 1348, 1349, 1627, 1714
Bme18I	GGWCC	3 cut(s)	344, 695, 871
BmeT110I	CYCGRG	1 cut(s)	1347
BmgBI	CACGTC	2 cut(s)	1026, 1495
BmgT120I	GGNCC	3 cut(s)	344, 695, 871
BmiI	GGNNCC	6 cut(s)	182, 306, 345, 388, 872, 1358
BmrFI	CCNGG	8 cut(s)	249, 508, 935, 1182, 1348, 1349, 1627, 1714
BmrI	ACTGGG	1 cut(s)	1392
BmsI	GCATC	8 cut(s)	130, 406, 460, 807, 1258, 1280, 1411, 1660
BmuI	ACTGGG	1 cut(s)	1392
BpiI	GAAGAC	1 cut(s)	1296
BpmI	CTGGAG	2 cut(s)	405, 1283
Bpu10I	CCTNAGC	2 cut(s)	866, 1136
Bpu14I	TTCGAA	1 cut(s)	411
BpuEI	CTTGAG	1 cut(s)	712
BpuMI	CCSGG	2 cut(s)	1348, 1349
BsaAI	YACGTR	1 cut(s)	1366
BsaBI	GATNNNNATC	2 cut(s)	365, 1076
BsaHI	GRCGYC	1 cut(s)	1314
BsaI	GGTCTC	2 cut(s)	716, 1337
BsaJI	CCNNGG	8 cut(s)	135, 248, 679, 1050, 1110, 1180, 1181, 1347
BsaWI	WCCGGW	3 cut(s)	301, 307, 1020
BsaXI	ACNNNNNCTCC	6 cut(s)	40, 70, 378, 408, 1494, 1524
Bsc4I	CCNNNNNNNGG	9 cut(s)	45, 502, 513, 617, 718, 970, 1353, 1366, 1417
Bse1I	ACTGG	4 cut(s)	622, 1360, 1387, 1460
Bse21I	CCTNAGG	1 cut(s)	582
Bse3DI	GCAATG	1 cut(s)	1637
Bse8I	GATNNNNATC	2 cut(s)	365, 1076
BseAI	TCCGGA	1 cut(s)	1020
BseBI	CCWGG	6 cut(s)	249, 508, 935, 1182, 1627, 1714
BseDI	CCNNGG	8 cut(s)	135, 248, 679, 1050, 1110, 1180, 1181, 1347
BseJI	GATNNNNATC	2 cut(s)	365, 1076
BseLI	CCNNNNNNNGG	9 cut(s)	45, 502, 513, 617, 718, 970, 1353, 1366, 1417
BseMI	GCAATG	1 cut(s)	1637
BseMII	CTCAG	4 cut(s)	573, 857, 1082, 1407
BseNI	ACTGG	4 cut(s)	622, 1360, 1387, 1460
BseRI	GAGGAG	5 cut(s)	61, 238, 1004, 1012, 1286
BseXI	GCAGC	5 cut(s)	1076, 1118, 1184, 1382, 1436
BseYI	CCCAGC	1 cut(s)	1192
BsgI	GTGCAG	1 cut(s)	1469
Bsh1236I	CGCG	2 cut(s)	1038, 1311
Bsh1285I	CGRYCG	1 cut(s)	949
BshFI	GGCC	4 cut(s)	38, 506, 639, 1306
BshNI	GGYRCC	1 cut(s)	304
BsiEI	CGRYCG	1 cut(s)	949
BsiHKAI	GWGCWC	2 cut(s)	301, 1500
BsiHKCI	CYCGRG	1 cut(s)	1347
BsiSI	CCGG	5 cut(s)	302, 308, 1021, 1336, 1348
BslFI	GGGAC	4 cut(s)	114, 357, 884, 1399
BslI	CCNNNNNNNGG	9 cut(s)	45, 502, 513, 617, 718, 970, 1353, 1366, 1417
BsmAI	GTCTC	2 cut(s)	716, 1337
BsmFI	GGGAC	4 cut(s)	114, 357, 884, 1399
BsmI	GAATGC	1 cut(s)	665
BsnI	GGCC	4 cut(s)	38, 506, 639, 1306
Bso31I	GGTCTC	2 cut(s)	716, 1337
BsoBI	CYCGRG	1 cut(s)	1347
Bsp119I	TTCGAA	1 cut(s)	411
Bsp1286I	GDGCHC	2 cut(s)	301, 1500
Bsp13I	TCCGGA	1 cut(s)	1020
Bsp143I	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
Bsp19I	CCATGG	1 cut(s)	1050
BspANI	GGCC	4 cut(s)	38, 506, 639, 1306
BspCNI	CTCAG	4 cut(s)	574, 858, 1081, 1406
BspEI	TCCGGA	1 cut(s)	1020
BspFNI	CGCG	2 cut(s)	1038, 1311
BspHI	TCATGA	1 cut(s)	993
BspLI	GGNNCC	6 cut(s)	182, 306, 345, 388, 872, 1358
BspPI	GGATC	8 cut(s)	251, 330, 847, 1082, 1351, 1364, 1472, 1714
BspT104I	TTCGAA	1 cut(s)	411
BspT107I	GGYRCC	1 cut(s)	304
BspTNI	GGTCTC	2 cut(s)	716, 1337
BsrBI	CCGCTC	2 cut(s)	51, 1640
BsrDI	GCAATG	1 cut(s)	1637
BsrI	ACTGG	4 cut(s)	622, 1360, 1387, 1460
BssECI	CCNNGG	8 cut(s)	135, 248, 679, 1050, 1110, 1180, 1181, 1347
BssMI	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
BssNI	GRCGYC	1 cut(s)	1314
BssT1I	CCWWGG	4 cut(s)	135, 679, 1050, 1110
Bst2UI	CCWGG	6 cut(s)	249, 508, 935, 1182, 1627, 1714
Bst4CI	ACNGT	8 cut(s)	105, 150, 423, 752, 811, 893, 965, 1490
Bst6I	CTCTTC	1 cut(s)	1255
BstACI	GRCGYC	1 cut(s)	1314
BstAPI	GCANNNNNTGC	1 cut(s)	1430
BstBAI	YACGTR	1 cut(s)	1366
BstBI	TTCGAA	1 cut(s)	411
BstC8I	GCNNGC	5 cut(s)	612, 1043, 1047, 1198, 1612
BstDEI	CTNAG	7 cut(s)	219, 582, 738, 866, 1068, 1136, 1393
BstDSI	CCRYGG	1 cut(s)	1050
BstENI	CCTNNNNNAGG	1 cut(s)	1415
BstFNI	CGCG	2 cut(s)	1038, 1311
BstHHI	GCGC	1 cut(s)	1038
BstKTI	GATC	8 cut(s)	259, 325, 366, 855, 1090, 1359, 1467, 1709
BstMAI	GTCTC	2 cut(s)	716, 1337
BstMBI	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
BstMCI	CGRYCG	1 cut(s)	949
BstMWI	GCNNNNNNNGC	7 cut(s)	21, 57, 1035, 1042, 1103, 1430, 1456
BstNI	CCWGG	6 cut(s)	249, 508, 935, 1182, 1627, 1714
BstNSI	RCATGY	2 cut(s)	1049, 1225
BstSCI	CCNGG	8 cut(s)	247, 506, 933, 1180, 1346, 1347, 1625, 1712
BstUI	CGCG	2 cut(s)	1038, 1311
BstV1I	GCAGC	5 cut(s)	1076, 1118, 1184, 1382, 1436
BstV2I	GAAGAC	1 cut(s)	1296
BstX2I	RGATCY	2 cut(s)	852, 1356
BstXI	CCANNNNNNTGG	2 cut(s)	686, 881
BstYI	RGATCY	2 cut(s)	852, 1356
Bsu36I	CCTNAGG	1 cut(s)	582
BsuRI	GGCC	4 cut(s)	38, 506, 639, 1306
BtgI	CCRYGG	1 cut(s)	1050
BtrI	CACGTC	2 cut(s)	1026, 1495
BtsI	GCAGTG	2 cut(s)	1428, 1466
BtsIMutI	CAGTG	5 cut(s)	419, 1380, 1401, 1428, 1466
Cac8I	GCNNGC	5 cut(s)	612, 1043, 1047, 1198, 1612
CaiI	CAGNNNCTG	2 cut(s)	1430, 1719
CciI	TCATGA	1 cut(s)	993
CfoI	GCGC	1 cut(s)	1038
Cfr13I	GGNCC	3 cut(s)	344, 695, 871
Cfr9I	CCCGGG	1 cut(s)	1347
CseI	GACGC	1 cut(s)	1303
Csp6I	GTAC	4 cut(s)	106, 229, 305, 1363
CviQI	GTAC	4 cut(s)	106, 229, 305, 1363
DdeI	CTNAG	7 cut(s)	219, 582, 738, 866, 1068, 1136, 1393
DpnI	GATC	8 cut(s)	258, 324, 365, 854, 1089, 1358, 1466, 1708
DpnII	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
Eam1104I	CTCTTC	1 cut(s)	1255
EarI	CTCTTC	1 cut(s)	1255
EciI	GGCGGA	3 cut(s)	78, 335, 419
Eco130I	CCWWGG	4 cut(s)	135, 679, 1050, 1110
Eco147I	AGGCCT	1 cut(s)	1306
Eco31I	GGTCTC	2 cut(s)	716, 1337
Eco32I	GATATC	1 cut(s)	1207
Eco47I	GGWCC	3 cut(s)	344, 695, 871
Eco57I	CTGAAG	1 cut(s)	485
Eco81I	CCTNAGG	1 cut(s)	582
Eco88I	CYCGRG	1 cut(s)	1347
EcoNI	CCTNNNNNAGG	1 cut(s)	1415
EcoO109I	RGGNCCY	1 cut(s)	344
EcoRII	CCWGG	6 cut(s)	247, 506, 933, 1180, 1625, 1712
EcoRV	GATATC	1 cut(s)	1207
EcoT14I	CCWWGG	4 cut(s)	135, 679, 1050, 1110
EcoT22I	ATGCAT	1 cut(s)	1273
ErhI	CCWWGG	4 cut(s)	135, 679, 1050, 1110
FaqI	GGGAC	4 cut(s)	114, 357, 884, 1399
FauNDI	CATATG	1 cut(s)	19
FbaI	TGATCA	1 cut(s)	363
FblI	GTMKAC	1 cut(s)	90
Fnu4HI	GCNGC	9 cut(s)	61, 1039, 1065, 1107, 1173, 1226, 1312, 1371, 1425
Fsp4HI	GCNGC	9 cut(s)	61, 1039, 1065, 1107, 1173, 1226, 1312, 1371, 1425
FspBI	CTAG	1 cut(s)	1325
GlaI	GCGC	1 cut(s)	1037
GluI	GCNGC	9 cut(s)	61, 1039, 1065, 1107, 1173, 1226, 1312, 1371, 1425
GsaI	CCCAGC	1 cut(s)	1196
GsuI	CTGGAG	2 cut(s)	405, 1283
HaeIII	GGCC	4 cut(s)	38, 506, 639, 1306
HapII	CCGG	5 cut(s)	302, 308, 1021, 1336, 1348
HgaI	GACGC	1 cut(s)	1303
HhaI	GCGC	1 cut(s)	1038
Hin1I	GRCGYC	1 cut(s)	1314
Hin6I	GCGC	1 cut(s)	1036
HinP1I	GCGC	1 cut(s)	1036
HincII	GTYRAC	1 cut(s)	91
HindII	GTYRAC	1 cut(s)	91
HindIII	AAGCTT	1 cut(s)	548
HinfI	GANTC	5 cut(s)	127, 186, 460, 982, 1072
HpaII	CCGG	5 cut(s)	302, 308, 1021, 1336, 1348
HphI	GGTGA	9 cut(s)	417, 617, 932, 1132, 1156, 1169, 1204, 1502, 1615
Hpy166II	GTNNAC	4 cut(s)	91, 520, 631, 1540
Hpy188III	TCNNGA	7 cut(s)	29, 80, 384, 994, 1021, 1300, 1667
Hpy8I	GTNNAC	4 cut(s)	91, 520, 631, 1540
Hpy99I	CGWCG	1 cut(s)	113
HpyAV	CCTTC	9 cut(s)	205, 230, 322, 460, 940, 1322, 1510, 1570, 1585
HpyCH4III	ACNGT	8 cut(s)	105, 150, 423, 752, 811, 893, 965, 1490
HpyCH4IV	ACGT	4 cut(s)	75, 1025, 1365, 1494
HpyF10VI	GCNNNNNNNGC	7 cut(s)	21, 57, 1035, 1042, 1103, 1430, 1456
HpyF3I	CTNAG	7 cut(s)	219, 582, 738, 866, 1068, 1136, 1393
HpySE526I	ACGT	4 cut(s)	75, 1025, 1365, 1494
Hsp92I	GRCGYC	1 cut(s)	1314
HspAI	GCGC	1 cut(s)	1036
Kpn2I	TCCGGA	1 cut(s)	1020
KpnI	GGTACC	1 cut(s)	308
Ksp22I	TGATCA	1 cut(s)	363
Kzo9I	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
LmnI	GCTCC	6 cut(s)	48, 304, 386, 483, 999, 1519
Lsp1109I	GCAGC	5 cut(s)	1076, 1118, 1184, 1382, 1436
LweI	GCATC	8 cut(s)	130, 406, 460, 807, 1258, 1280, 1411, 1660
MaeI	CTAG	1 cut(s)	1325
MaeII	ACGT	4 cut(s)	75, 1025, 1365, 1494
MaeIII	GTNAC	2 cut(s)	423, 1490
MalI	GATC	8 cut(s)	258, 324, 365, 854, 1089, 1358, 1466, 1708
MbiI	CCGCTC	2 cut(s)	51, 1640
MboI	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
MflI	RGATCY	2 cut(s)	852, 1356
MhlI	GDGCHC	2 cut(s)	301, 1500
MluCI	AATT	2 cut(s)	173, 286
MlyI	GAGTC	1 cut(s)	976
MmeI	TCCRAC	3 cut(s)	180, 188, 1464
Mph1103I	ATGCAT	1 cut(s)	1273
MroI	TCCGGA	1 cut(s)	1020
MroXI	GAANNNNTTC	1 cut(s)	825
MseI	TTAA	1 cut(s)	1587
MspA1I	CMGCKG	1 cut(s)	833
MspI	CCGG	5 cut(s)	302, 308, 1021, 1336, 1348
MspR9I	CCNGG	8 cut(s)	249, 508, 935, 1182, 1348, 1349, 1627, 1714
Mva1269I	GAATGC	1 cut(s)	665
MvaI	CCWGG	6 cut(s)	249, 508, 935, 1182, 1627, 1714
MvnI	CGCG	2 cut(s)	1038, 1311
MwoI	GCNNNNNNNGC	7 cut(s)	21, 57, 1035, 1042, 1103, 1430, 1456
NciI	CCSGG	2 cut(s)	1348, 1349
NcoI	CCATGG	1 cut(s)	1050
NdeI	CATATG	1 cut(s)	19
NdeII	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
NlaIV	GGNNCC	6 cut(s)	182, 306, 345, 388, 872, 1358
NmuCI	GTSAC	2 cut(s)	423, 1490
NsiI	ATGCAT	1 cut(s)	1273
NspI	RCATGY	2 cut(s)	1049, 1225
NspV	TTCGAA	1 cut(s)	411
PaeI	GCATGC	1 cut(s)	1049
PagI	TCATGA	1 cut(s)	993
PasI	CCCWGGG	1 cut(s)	1181
PceI	AGGCCT	1 cut(s)	1306
PcsI	WCGNNNNNNNCGW	1 cut(s)	1315
PctI	GAATGC	1 cut(s)	665
PdmI	GAANNNNTTC	1 cut(s)	825
PfeI	GAWTC	4 cut(s)	127, 186, 460, 1072
PflMI	CCANNNNNTGG	1 cut(s)	1366
PkrI	GCNGC	9 cut(s)	62, 1040, 1066, 1108, 1174, 1227, 1313, 1372, 1426
PleI	GAGTC	1 cut(s)	976
PpsI	GAGTC	1 cut(s)	976
Ppu21I	YACGTR	1 cut(s)	1366
PpuMI	RGGWCCY	1 cut(s)	344
Psp5II	RGGWCCY	1 cut(s)	344
Psp6I	CCWGG	6 cut(s)	247, 506, 933, 1180, 1625, 1712
PspFI	CCCAGC	1 cut(s)	1192
PspGI	CCWGG	6 cut(s)	247, 506, 933, 1180, 1625, 1712
PspN4I	GGNNCC	6 cut(s)	182, 306, 345, 388, 872, 1358
PspPI	GGNCC	3 cut(s)	344, 695, 871
PspPPI	RGGWCCY	1 cut(s)	344
PstNI	CAGNNNCTG	2 cut(s)	1430, 1719
PsuI	RGATCY	2 cut(s)	852, 1356
PvuII	CAGCTG	1 cut(s)	833
RsaI	GTAC	4 cut(s)	107, 230, 306, 1364
RsaNI	GTAC	4 cut(s)	106, 229, 305, 1363
SalI	GTCGAC	1 cut(s)	89
SaqAI	TTAA	1 cut(s)	1587
SatI	GCNGC	9 cut(s)	61, 1039, 1065, 1107, 1173, 1226, 1312, 1371, 1425
Sau3AI	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
Sau96I	GGNCC	3 cut(s)	344, 695, 871
SchI	GAGTC	1 cut(s)	976
ScrFI	CCNGG	8 cut(s)	249, 508, 935, 1182, 1348, 1349, 1627, 1714
SduI	GDGCHC	2 cut(s)	301, 1500
SfaNI	GCATC	8 cut(s)	130, 406, 460, 807, 1258, 1280, 1411, 1660
SfuI	TTCGAA	1 cut(s)	411
SinI	GGWCC	3 cut(s)	344, 695, 871
SmaI	CCCGGG	1 cut(s)	1349
SmlI	CTYRAG	1 cut(s)	727
SmoI	CTYRAG	1 cut(s)	727
SphI	GCATGC	1 cut(s)	1049
Sse9I	AATT	2 cut(s)	173, 286
SseBI	AGGCCT	1 cut(s)	1306
SspMI	CTAG	1 cut(s)	1325
StuI	AGGCCT	1 cut(s)	1306
StyD4I	CCNGG	8 cut(s)	247, 506, 933, 1180, 1346, 1347, 1625, 1712
StyI	CCWWGG	4 cut(s)	135, 679, 1050, 1110
TaaI	ACNGT	8 cut(s)	105, 150, 423, 752, 811, 893, 965, 1490
TaiI	ACGT	4 cut(s)	78, 1028, 1368, 1497
TaqI	TCGA	2 cut(s)	90, 411
TasI	AATT	2 cut(s)	173, 286
TauI	GCSGC	4 cut(s)	63, 1041, 1228, 1314
TfiI	GAWTC	4 cut(s)	127, 186, 460, 1072
Tru1I	TTAA	1 cut(s)	1587
Tru9I	TTAA	1 cut(s)	1587
TscAI	CASTG	5 cut(s)	426, 1387, 1401, 1435, 1466
TseFI	GTSAC	2 cut(s)	423, 1490
TseI	GCWGC	5 cut(s)	1064, 1106, 1172, 1370, 1424
Tsp45I	GTSAC	2 cut(s)	423, 1490
TspDTI	ATGAA	6 cut(s)	249, 262, 388, 586, 982, 1116
TspGWI	ACGGA	1 cut(s)	126
TspMI	CCCGGG	1 cut(s)	1347
TspRI	CASTG	5 cut(s)	426, 1387, 1401, 1435, 1466
Van91I	CCANNNNNTGG	1 cut(s)	1366
VpaK11BI	GGWCC	3 cut(s)	344, 695, 871
XagI	CCTNNNNNAGG	1 cut(s)	1415
XapI	RAATTY	1 cut(s)	173
XceI	RCATGY	2 cut(s)	1049, 1225
XcmI	CCANNNNNNNNNTGG	2 cut(s)	397, 1057
XmaI	CCCGGG	1 cut(s)	1347
XmiI	GTMKAC	1 cut(s)	90
XmnI	GAANNNNTTC	1 cut(s)	825
XspI	CTAG	1 cut(s)	1325
Zsp2I	ATGCAT	1 cut(s)	1273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.