Rmu_sc0030109.1_g000001

Belongs to the malate synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0030109.1
OG0014194
Physical Location & Seq
Forward (+)
1 .. 1961
1961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0030109.1_g000001.1.cds

Sequence Viewer

Length: 1307 bp
atgcactttcaccaagctgggagaatctaataagaggccaagtgaacctgaaagatgctgttgatgggagcataactttccatgataaggccagaaacagagtctacaagctcaacgatcacaacatagccaagctttttgtgcgtccaagaggttggcacttgcctgaggctcatattctcattgatggtgagccggcaactggttgccttgtcgactttggtctctacttctaccacaactatgcagcattccgccgcacccaaggtgaagggtctggacctttcttctacctccctaaaatggagcattcaagggaagcgaagatatggaattgtgtgttcgagaaggcagagaagatggtagggatagagagaggaagcatcagagccactgttctgatcgaaacacttccagctgtgtttcaaatggatgaaatcctgtatgagcttagggatcactctgttggtttgaactgtggaagatgggattacattttcagctatgtcaagaccttccaggctcaccctgaccgcctattgcctgaccgggttctagttggcatgaatcaacatttcatgaggagttactctgatctcctcatcaggacctgccataggcgcggtgcccacgccatgggaggcatggcagctcagattccaattagagatgatccagtagcaaatgaagcagcattggatttggtgaggaaggacaagctaagagaagtgaaagcaggacatgatggaacttgggcagcacatccaggactaatcccagcctgcatggaaatcttcacaaacaacatgggccgcaacacccccaaccaaatccaaaccatgaggcgagaagatgcatcctccataaccgaagaagacctcttgcagaggcctagaggagtccgaaccctggagggtctccggctgaacacccgggtggggatccagtacctggcagcatggctaaccggagctggttcggtgccactttacaatctcatggaagatgctgcaacagctgagataagcagggttcagaactggcagtggatcaagtatggagtggagttggatggggatggacttggagtgagagtggggaaggaactgtttgggagagtggtggaagacgaaatggagaggattgaaagagaggttgggaaggacaaatttaagaagggaatgtacagagaggcttgcaagatgttcgcaaggcaatgcacagcgcaaactctagacgattttctgactctcgacgcttataatcatatcgtcttgcaccaccccaaggcatcatccaggctctaa

Protein Analysis

434

Amino Acids

49.47

Weight (kDa)

7.8

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03860 AT5G03860
fragaria_vesca FvH4_5g07710
malus_domestica MD06G1147700.v1.1
prunus_persica Prupe.5G154600_v2.0.a1
pyrus_communis pycom06g13710
rosa_chinensis RchiOBHm_Chr7g0190721
rosa_laevigata RLG00000004501
rosa_multiflora Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii Rroxscaffold_3G00264350
rosa_rugosa Rorug06G0506700
rosa_samantha Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana Rw7G009890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1262
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 2 cut(s) 625, 981
AccB7I CCANNNNNTGG 2 cut(s) 636, 951
AccI GTMKAC 2 cut(s) 104, 215
AccII CGCG 1 cut(s) 623
AciI CCGC 5 cut(s) 255, 258, 534, 623, 813
AclWI GGATC 5 cut(s) 464, 667, 936, 949, 1057
AcsI RAATTY 1 cut(s) 1168
AfaI GTAC 2 cut(s) 949, 1186
AfiI CCNNNNNNNGG 9 cut(s) 87, 202, 303, 617, 636, 909, 938, 951, 1287
AgsI TTSAA 4 cut(s) 314, 427, 474, 1147
AjnI CCWGG 5 cut(s) 518, 765, 908, 950, 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 1139, 1171
AleI CACNNNNGTG 1 cut(s) 934
Alw26I GTCTC 2 cut(s) 229, 922
AlwI GGATC 5 cut(s) 464, 667, 936, 949, 1057
AlwNI CAGNNNCTG 2 cut(s) 611, 951
Ama87I CYCGRG 1 cut(s) 932
AoxI GGCC 4 cut(s) 36, 89, 810, 889
ApeKI GCWGC 6 cut(s) 247, 649, 691, 757, 955, 1009
ApoI RAATTY 1 cut(s) 1168
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 2 cut(s) 623, 1227
AspS9I GGNCC 3 cut(s) 280, 608, 810
AsuC2I CCSGG 3 cut(s) 550, 933, 934
AsuHPI GGTGA 4 cut(s) 202, 280, 517, 717
AvaI CYCGRG 1 cut(s) 932
AvaII GGWCC 2 cut(s) 280, 608
AxyI CCTNAGG 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 630
BamHI GGATCC 1 cut(s) 941
BanI GGYRCC 2 cut(s) 625, 981
BarI GAAGNNNNNNTAC 2 cut(s) 1168, 1200
BbsI GAAGAC 2 cut(s) 881, 1133
BbvI GCAGC 6 cut(s) 259, 661, 703, 769, 967, 996
BccI CCATC 7 cut(s) 58, 181, 354, 479, 739, 1066, 1072
BcgI CGANNNNNNTGC 2 cut(s) 1188, 1222
BciT130I CCWGG 5 cut(s) 520, 767, 910, 952, 1299
BcnI CCSGG 3 cut(s) 550, 933, 934
BcoDI GTCTC 2 cut(s) 229, 922
BfaI CTAG 3 cut(s) 556, 893, 1234
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 8 cut(s) 248, 258, 650, 692, 758, 813, 956, 1010
BlsI GCNGC 8 cut(s) 249, 259, 651, 693, 759, 814, 957, 1011
Bme1390I CCNGG 8 cut(s) 520, 550, 767, 910, 933, 934, 952, 1299
Bme18I GGWCC 2 cut(s) 280, 608
BmeT110I CYCGRG 1 cut(s) 932
BmgT120I GGNCC 3 cut(s) 280, 608, 810
BmiI GGNNCC 3 cut(s) 627, 943, 983
BmrFI CCNGG 8 cut(s) 520, 550, 767, 910, 933, 934, 952, 1299
BmsI GCATC 6 cut(s) 45, 392, 843, 865, 996, 1300
BoxI GACNNNNGTC 1 cut(s) 221
BpiI GAAGAC 2 cut(s) 881, 1133
BpmI CTGGAG 1 cut(s) 931
Bpu10I CCTNAGC 1 cut(s) 451
BpuMI CCSGG 3 cut(s) 550, 933, 934
BsaI GGTCTC 2 cut(s) 229, 922
BsaJI CCNNGG 5 cut(s) 264, 635, 908, 932, 1286
BsaWI WCCGGW 1 cut(s) 967
BsaXI ACNNNNNCTCC 2 cut(s) 1079, 1109
Bsc4I CCNNNNNNNGG 9 cut(s) 87, 202, 303, 617, 636, 909, 938, 951, 1287
Bse118I RCCGGY 1 cut(s) 195
Bse1I ACTGG 4 cut(s) 207, 676, 945, 1045
Bse21I CCTNAGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 1222
BseBI CCWGG 5 cut(s) 520, 767, 910, 952, 1299
BseDI CCNNGG 5 cut(s) 264, 635, 908, 932, 1286
BseGI GGATG 6 cut(s) 438, 762, 856, 1077, 1083, 1294
BseLI CCNNNNNNNGG 9 cut(s) 87, 202, 303, 617, 636, 909, 938, 951, 1287
BseMI GCAATG 1 cut(s) 1222
BseMII CTCAG 3 cut(s) 158, 667, 1010
BseNI ACTGG 4 cut(s) 207, 676, 945, 1045
BseRI GAGGAG 3 cut(s) 589, 597, 911
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 6 cut(s) 259, 661, 703, 769, 967, 996
BseYI CCCAGC 2 cut(s) 17, 777
Bsh1236I CGCG 1 cut(s) 623
BshFI GGCC 4 cut(s) 38, 91, 812, 891
BshNI GGYRCC 2 cut(s) 625, 981
BsiHKCI CYCGRG 1 cut(s) 932
BsiSI CCGG 5 cut(s) 196, 549, 921, 933, 968
BslI CCNNNNNNNGG 9 cut(s) 87, 202, 303, 617, 636, 909, 938, 951, 1287
BsmAI GTCTC 2 cut(s) 229, 922
BsmI GAATGC 2 cut(s) 250, 309
BsnI GGCC 4 cut(s) 38, 91, 812, 891
Bso31I GGTCTC 2 cut(s) 229, 922
BsoBI CYCGRG 1 cut(s) 932
Bsp1286I GDGCHC 1 cut(s) 630
Bsp1407I TGTACA 1 cut(s) 1184
Bsp143I GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
Bsp19I CCATGG 1 cut(s) 635
BspACI CCGC 5 cut(s) 255, 258, 534, 623, 813
BspANI GGCC 4 cut(s) 38, 91, 812, 891
BspCNI CTCAG 3 cut(s) 159, 666, 1011
BspFNI CGCG 1 cut(s) 623
BspHI TCATGA 1 cut(s) 578
BspLI GGNNCC 3 cut(s) 627, 943, 983
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 5 cut(s) 464, 667, 936, 949, 1057
BspT107I GGYRCC 2 cut(s) 625, 981
BspTNI GGTCTC 2 cut(s) 229, 922
BsrDI GCAATG 1 cut(s) 1222
BsrFI RCCGGY 1 cut(s) 195
BsrGI TGTACA 1 cut(s) 1184
BsrI ACTGG 4 cut(s) 207, 676, 945, 1045
BssAI RCCGGY 1 cut(s) 195
BssECI CCNNGG 5 cut(s) 264, 635, 908, 932, 1286
BssMI GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
BssT1I CCWWGG 3 cut(s) 264, 635, 1286
Bst2UI CCWGG 5 cut(s) 520, 767, 910, 952, 1299
Bst4CI ACNGT 3 cut(s) 396, 478, 1109
BstAUI TGTACA 1 cut(s) 1184
BstC8I GCNNGC 3 cut(s) 197, 783, 1197
BstDEI CTNAG 5 cut(s) 167, 451, 653, 721, 1019
BstDSI CCRYGG 1 cut(s) 635
BstENI CCTNNNNNAGG 1 cut(s) 615
BstF5I GGATG 6 cut(s) 438, 762, 856, 1077, 1083, 1294
BstFNI CGCG 1 cut(s) 623
BstHHI GCGC 2 cut(s) 623, 1227
BstKTI GATC 7 cut(s) 120, 404, 459, 597, 675, 944, 1052
BstMAI GTCTC 2 cut(s) 229, 922
BstMBI GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
BstMWI GCNNNNNNNGC 4 cut(s) 141, 620, 688, 1015
BstNI CCWGG 5 cut(s) 520, 767, 910, 952, 1299
BstPAI GACNNNNGTC 1 cut(s) 221
BstSCI CCNGG 8 cut(s) 518, 548, 765, 908, 931, 932, 950, 1297
BstSLI GKGCMC 1 cut(s) 630
BstUI CGCG 1 cut(s) 623
BstV1I GCAGC 6 cut(s) 259, 661, 703, 769, 967, 996
BstV2I GAAGAC 2 cut(s) 881, 1133
BstX2I RGATCY 1 cut(s) 941
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 941
Bsu36I CCTNAGG 1 cut(s) 167
BsuRI GGCC 4 cut(s) 38, 91, 812, 891
BtgI CCRYGG 1 cut(s) 635
BtsCI GGATG 6 cut(s) 438, 762, 856, 1077, 1083, 1294
BtsI GCAGTG 1 cut(s) 1051
BtsIMutI CAGTG 2 cut(s) 392, 1051
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 3 cut(s) 197, 783, 1197
CaiI CAGNNNCTG 2 cut(s) 611, 951
CciI TCATGA 1 cut(s) 578
CfoI GCGC 2 cut(s) 623, 1227
Cfr10I RCCGGY 1 cut(s) 195
Cfr13I GGNCC 3 cut(s) 280, 608, 810
Cfr9I CCCGGG 1 cut(s) 932
CseI GACGC 2 cut(s) 133, 1264
Csp6I GTAC 2 cut(s) 948, 1185
CviQI GTAC 2 cut(s) 948, 1185
DdeI CTNAG 5 cut(s) 167, 451, 653, 721, 1019
DpnI GATC 7 cut(s) 119, 403, 458, 596, 674, 943, 1051
DpnII GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
EciI GGCGGA 1 cut(s) 244
Eco130I CCWWGG 3 cut(s) 264, 635, 1286
Eco147I AGGCCT 1 cut(s) 891
Eco31I GGTCTC 2 cut(s) 229, 922
Eco47I GGWCC 2 cut(s) 280, 608
Eco81I CCTNAGG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 932
EcoNI CCTNNNNNAGG 1 cut(s) 615
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 5 cut(s) 518, 765, 908, 950, 1297
EcoT14I CCWWGG 3 cut(s) 264, 635, 1286
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 3 cut(s) 264, 635, 1286
FblI GTMKAC 2 cut(s) 104, 215
Fnu4HI GCNGC 8 cut(s) 248, 258, 650, 692, 758, 813, 956, 1010
FokI GGATG 6 cut(s) 445, 749, 843, 1084, 1090, 1281
Fsp4HI GCNGC 8 cut(s) 248, 258, 650, 692, 758, 813, 956, 1010
FspBI CTAG 3 cut(s) 556, 893, 1234
GlaI GCGC 2 cut(s) 622, 1226
GluI GCNGC 8 cut(s) 248, 258, 650, 692, 758, 813, 956, 1010
GsaI CCCAGC 2 cut(s) 21, 781
GsuI CTGGAG 1 cut(s) 931
HaeIII GGCC 4 cut(s) 38, 91, 812, 891
HapII CCGG 5 cut(s) 196, 549, 921, 933, 968
HgaI GACGC 2 cut(s) 133, 1264
HhaI GCGC 2 cut(s) 623, 1227
Hin6I GCGC 2 cut(s) 621, 1225
HinP1I GCGC 2 cut(s) 621, 1225
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 133
HinfI GANTC 6 cut(s) 24, 101, 567, 657, 899, 1248
HpaII CCGG 5 cut(s) 196, 549, 921, 933, 968
HphI GGTGA 4 cut(s) 202, 280, 517, 717
Hpy166II GTNNAC 3 cut(s) 45, 105, 216
Hpy188I TCNGA 7 cut(s) 388, 401, 594, 656, 904, 1037, 1247
Hpy188III TCNNGA 7 cut(s) 278, 345, 510, 579, 606, 1234, 1252
Hpy8I GTNNAC 3 cut(s) 45, 105, 216
Hpy99I CGWCG 1 cut(s) 1258
HpyAV CCTTC 7 cut(s) 265, 342, 525, 705, 1095, 1155, 1170
HpyCH4III ACNGT 3 cut(s) 396, 478, 1109
HpyCH4V TGCA 9 cut(s) 4, 247, 785, 856, 885, 1012, 1199, 1220, 1278
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 620, 688, 1015
HpyF3I CTNAG 5 cut(s) 167, 451, 653, 721, 1019
HspAI GCGC 2 cut(s) 621, 1225
KroI GCCGGC 1 cut(s) 195
KroNI GCCGGC 1 cut(s) 197
Kzo9I GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
LmnI GCTCC 3 cut(s) 68, 306, 970
Lsp1109I GCAGC 6 cut(s) 259, 661, 703, 769, 967, 996
LweI GCATC 6 cut(s) 45, 392, 843, 865, 996, 1300
MaeI CTAG 3 cut(s) 556, 893, 1234
MaeIII GTNAC 1 cut(s) 586
MalI GATC 7 cut(s) 119, 403, 458, 596, 674, 943, 1051
MboI GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
MflI RGATCY 1 cut(s) 941
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 3 cut(s) 333, 662, 1168
MlyI GAGTC 3 cut(s) 110, 908, 1242
MmeI TCCRAC 1 cut(s) 1049
Mph1103I ATGCAT 1 cut(s) 858
MroNI GCCGGC 1 cut(s) 195
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 1 cut(s) 1172
MslI CAYNNNNRTG 2 cut(s) 242, 934
MspA1I CMGCKG 2 cut(s) 418, 1018
MspI CCGG 5 cut(s) 196, 549, 921, 933, 968
MspR9I CCNGG 8 cut(s) 520, 550, 767, 910, 933, 934, 952, 1299
Mva1269I GAATGC 2 cut(s) 250, 309
MvaI CCWGG 5 cut(s) 520, 767, 910, 952, 1299
MvnI CGCG 1 cut(s) 623
MwoI GCNNNNNNNGC 4 cut(s) 141, 620, 688, 1015
NaeI GCCGGC 1 cut(s) 197
NciI CCSGG 3 cut(s) 550, 933, 934
NcoI CCATGG 1 cut(s) 635
NdeII GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
NgoMIV GCCGGC 1 cut(s) 195
NlaIV GGNNCC 3 cut(s) 627, 943, 983
NsiI ATGCAT 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 934
PagI TCATGA 1 cut(s) 578
PceI AGGCCT 1 cut(s) 891
PctI GAATGC 2 cut(s) 250, 309
PdiI GCCGGC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 3 cut(s) 24, 567, 657
PflMI CCANNNNNTGG 2 cut(s) 636, 951
PfoI TCCNGGA 1 cut(s) 765
PkrI GCNGC 8 cut(s) 249, 259, 651, 693, 759, 814, 957, 1011
PleI GAGTC 3 cut(s) 109, 907, 1242
PpsI GAGTC 3 cut(s) 109, 907, 1242
PpuMI RGGWCCY 1 cut(s) 608
PshAI GACNNNNGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 1262
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 5 cut(s) 518, 765, 908, 950, 1297
PspFI CCCAGC 2 cut(s) 17, 777
PspGI CCWGG 5 cut(s) 518, 765, 908, 950, 1297
PspN4I GGNNCC 3 cut(s) 627, 943, 983
PspPI GGNCC 3 cut(s) 280, 608, 810
PspPPI RGGWCCY 1 cut(s) 608
PstNI CAGNNNCTG 2 cut(s) 611, 951
PsuI RGATCY 1 cut(s) 941
PvuII CAGCTG 2 cut(s) 418, 1018
RsaI GTAC 2 cut(s) 949, 1186
RsaNI GTAC 2 cut(s) 948, 1185
RseI CAYNNNNRTG 2 cut(s) 242, 934
SalI GTCGAC 1 cut(s) 214
SaqAI TTAA 1 cut(s) 1172
SatI GCNGC 8 cut(s) 248, 258, 650, 692, 758, 813, 956, 1010
Sau3AI GATC 7 cut(s) 117, 401, 456, 594, 672, 941, 1049
Sau96I GGNCC 3 cut(s) 280, 608, 810
SchI GAGTC 3 cut(s) 110, 908, 1242
ScrFI CCNGG 8 cut(s) 520, 550, 767, 910, 933, 934, 952, 1299
SduI GDGCHC 1 cut(s) 630
SfaNI GCATC 6 cut(s) 45, 392, 843, 865, 996, 1300
SinI GGWCC 2 cut(s) 280, 608
SmaI CCCGGG 1 cut(s) 934
SmiMI CAYNNNNRTG 2 cut(s) 242, 934
Sse9I AATT 3 cut(s) 333, 662, 1168
SseBI AGGCCT 1 cut(s) 891
SsiI CCGC 5 cut(s) 255, 258, 534, 623, 813
SspMI CTAG 3 cut(s) 556, 893, 1234
StuI AGGCCT 1 cut(s) 891
StyD4I CCNGG 8 cut(s) 518, 548, 765, 908, 931, 932, 950, 1297
StyI CCWWGG 3 cut(s) 264, 635, 1286
TaaI ACNGT 3 cut(s) 396, 478, 1109
TaqI TCGA 4 cut(s) 215, 344, 404, 1253
TasI AATT 3 cut(s) 333, 662, 1168
TatI WGTACW 1 cut(s) 1184
TauI GCSGC 2 cut(s) 260, 815
TfiI GAWTC 3 cut(s) 24, 567, 657
Tru1I TTAA 1 cut(s) 1172
Tru9I TTAA 1 cut(s) 1172
TscAI CASTG 2 cut(s) 399, 1051
TseI GCWGC 6 cut(s) 247, 649, 691, 757, 955, 1009
TspDTI ATGAA 4 cut(s) 449, 567, 580, 701
TspMI CCCGGG 1 cut(s) 932
TspRI CASTG 2 cut(s) 399, 1051
Van91I CCANNNNNTGG 2 cut(s) 636, 951
VpaK11BI GGWCC 2 cut(s) 280, 608
XagI CCTNNNNNAGG 1 cut(s) 615
XapI RAATTY 1 cut(s) 1168
XbaI TCTAGA 1 cut(s) 1233
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XmaI CCCGGG 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 104, 215
XmnI GAANNNNTTC 1 cut(s) 410
XspI CTAG 3 cut(s) 556, 893, 1234
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.