Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0014194

Physical Location & Seq

Genomic Coordinates Reverse (-)

58031104 .. 58034633

Gene Structure 3530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00264350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1959 bp

ATGATGGGAGTTGGCTCGTACGGGTACCCTGCACCAGCGACGAACATGAACACCGGTGCAAGAGGGGGCTACGATGCCCCCGAAGGAGTTGAAATTCGGGGACGGTATGACCAAGAGTATGCGAAGATTCTGAGCAAGGAGGCTTTGCAGTTCGTTGTGGAGTTGCAGAGGGAGTTCAGGAACAGGATTAAGTATGCATTGGAGTGTAGGAAGGAGGCTAAGAGGAGGTACAATGAGGGTGGTCTACCCGGGTTTGACCCCGCCACGAAGTTCATAAGGGAAGGGGATTGGGTTTGTGCACCGGTCCCGAAAGCGGTGGCTGATCGGAGGGTGGAGATTACAGGACCTGTGGAGAGGAAGATGGTCATCAATGCTCTCAATTCTGGAGCAAAGGTTTTCATGGCTGATTTTGAAGATGCACTTTCACCGAGCTGGGAGAATCTAATAAGAGGCCAAGTGAACCTGAAAGATGCTGTGGATGGGAGCATAACTTTCCATGACAAGGCCAGGAACAGAGTCTACAAGCTCAATGATCACAACATAGCCAAGCTTTTTGTGCGTCCAAGAGGTTGGCACTTGCCTGAGGCTCATATTCTCATTGATGGTGAGCCGGCAACTGGTTGCCTTGTGGACTTTGGTCTCTACTTTTACCACAACTATGCAGCATTCCGCCGCACCCAAGGTGAAGGGGAAGCGAAGATATGGAATTGTGTGTTCGAGAAGGCAGAGAAGATGGTAGGGATAGAGAGAGGAAGCATCAGAGCCACTGTTCTGATTGAAACACTTCCAGCTGTGTTTCAAATGGATGAAATCCTGTATGAGCTTAGGGATCACTCTGTTGGTTTGAACTGTGGAAGATGGGATTACATTTTCAGCTATGTCAAGACCTTCCAGGCTCACCCTGACCGCCTATTGCCTGACCGGGTTCTAGTTGGCATGAATCAACATTTCATGAGGAGTTACTCTGATCTCCTCATCAGGACCTGCCATAGGCGCGGTGTCCACGCCATGGGAGGCATGGCAGCTCAGATTCCAATTAGAGATGATCCAGTAGCAAATGAAGCAGCATTGGATTTGGTGAGGAAGGACAAGCTAAGAGAAGTGAAAGCAGGACATGATGGAACTTGGGCAGCACATCCAGGACTAATCCCAGCCTGCATGGAAATCTTCACAAACAACATGGGCCACAACACCCCCAACCAAATCCAAACCATGAGGCGAGAAGATGCATCCTCCATAACCGAAGAAGACCTCTTGCAGAGGCCTAGAGGAGTCCGAACCCTGGAGGGTCTCCGGCTGAACACCCGGGTGGGGATCCAGTACCTGGCAGCATGGCTAACTGGAGCTGGCTCGGTGCCACTTTACAATCTCATGGAAGATGCTGCAACAGCTGAGATAAGCAGGGTTCAGAACTGGCAGTGGATCAAGTATGGAGTGGAGTTGGATGGAGATGGACTTGGAGTGAGAGTGGGGAAGGAGCTGTTTGGTAGAGTGGTGGAAGACGAAATGGAGAGGATTGAAAGAGAGGTCGGGAAGGACAAATTTAAGAAGGGAATGTACAAAGAGGCTTGCAAGATGTTCACAAGGCAATGCACAGCACGAACTCTAGACGATTTTCTGACTCTCGATGCTTACAATCATATCGCTCGGCTTCCCCGGCATCCTAAGATTCACGAGCGAGTCACCGAGTCGGCCGCCGAGTTGGAGGCGAGGCAAGCGCAGCTCGGAGACCCAAGTAGTATCGTAGCAAAAGACGCGGCACCGGTTGCGGGGAGCGGAGGGCGTGGAGAAATCCCGGCAGTTCCATGCGCTAATCGGATCCCGGAGGTTGAGCCCGAAGGTGGTGAGGGCGTGGATCTTGGCCGGGCTTTCGGGGTTGCGCTAGGCGGGCGAGAGAAAGAAGGGTGCGCCGCAAAGCGCGAGAAAGAAAAGAAGAAGCGCATCCATAATGGGTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004474 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016740 GO:0016746 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00620 ko00630 ko01100 ko01110 ko01120 ko01200 map00620 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

Malate_synthase

Protein Analysis

652

Amino Acids

73.1

Weight (kDa)

7.92

Isoelectric Point (pI)

41.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_N	PF20656	28 - 90	8.4e-21	Malate synthase, N-terminal domain
MS_TIM-barrel	PF01274	183 - 420	3.8e-104	Malate synthase, TIM barrel domain
MS_C	PF20659	426 - 548	9.2e-44	Malate synthase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03860 AT5G03860
fragaria_vesca	FvH4_5g07710
malus_domestica	MD06G1147700.v1.1
prunus_persica	Prupe.5G154600_v2.0.a1
pyrus_communis	pycom06g13710
rosa_chinensis	RchiOBHm_Chr7g0190721
rosa_laevigata	RLG00000004501
rosa_multiflora	Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264350
rosa_rugosa	Rorug06G0506700
rosa_samantha	Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana	Rw7G009890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	992
Acc65I	GGTACC	1 cut(s)	24
AccB1I	GGYRCC	3 cut(s)	24, 1354, 1759
AccB7I	CCANNNNNTGG	2 cut(s)	1009, 1324
AccBSI	CCGCTC	1 cut(s)	1776
AccI	GTMKAC	2 cut(s)	244, 519
AccII	CGCG	3 cut(s)	996, 1757, 1920
AclWI	GGATC	8 cut(s)	837, 1040, 1309, 1322, 1430, 1813, 1826, 1863
AcoI	YGGCCR	2 cut(s)	1692, 1861
AcsI	RAATTY	2 cut(s)	93, 1541
AfaI	GTAC	5 cut(s)	20, 26, 230, 1322, 1559
AgeI	ACCGGT	3 cut(s)	53, 301, 1762
AgsI	TTSAA	6 cut(s)	92, 413, 779, 800, 847, 1520
AjnI	CCWGG	5 cut(s)	506, 891, 1138, 1281, 1323
AleI	CACNNNNGTG	1 cut(s)	1307
Alw21I	GWGCWC	1 cut(s)	301
Alw26I	GTCTC	3 cut(s)	644, 1295, 1722
Alw44I	GTGCAC	1 cut(s)	297
AlwI	GGATC	8 cut(s)	837, 1040, 1309, 1322, 1430, 1813, 1826, 1863
AlwNI	CAGNNNCTG	3 cut(s)	347, 984, 1324
Ama87I	CYCGRG	2 cut(s)	248, 1305
AoxI	GGCC	6 cut(s)	451, 504, 1183, 1262, 1692, 1861
ApaLI	GTGCAC	1 cut(s)	297
ApeKI	GCWGC	7 cut(s)	662, 1022, 1064, 1130, 1328, 1382, 1720
ApoI	RAATTY	2 cut(s)	93, 1541
AsiGI	ACCGGT	3 cut(s)	53, 301, 1762
Asp700I	GAANNNNTTC	1 cut(s)	783
Asp718I	GGTACC	1 cut(s)	24
AspLEI	GCGC	7 cut(s)	996, 1720, 1811, 1882, 1910, 1920, 1941
AspS9I	GGNCC	4 cut(s)	304, 344, 981, 1183
AsuC2I	CCSGG	9 cut(s)	249, 250, 923, 1306, 1307, 1657, 1796, 1823, 1865
AsuHPI	GGTGA	7 cut(s)	417, 617, 695, 890, 1090, 1675, 1856
AvaI	CYCGRG	2 cut(s)	248, 1305
AvaII	GGWCC	3 cut(s)	304, 344, 981
AxyI	CCTNAGG	1 cut(s)	582
BaeGI	GKGCMC	1 cut(s)	301
BamHI	GGATCC	2 cut(s)	1314, 1818
BanI	GGYRCC	3 cut(s)	24, 1354, 1759
BanII	GRGCYC	1 cut(s)	1836
BarI	GAAGNNNNNNTAC	2 cut(s)	1541, 1573
BauI	CACGAG	1 cut(s)	1673
BbsI	GAAGAC	2 cut(s)	1254, 1506
Bbv12I	GWGCWC	1 cut(s)	301
BbvI	GCAGC	7 cut(s)	674, 1034, 1076, 1142, 1340, 1369, 1732
BccI	CCATC	8 cut(s)	355, 473, 596, 727, 852, 1112, 1439, 1445
BciT130I	CCWGG	5 cut(s)	508, 893, 1140, 1283, 1325
BclI	TGATCA	1 cut(s)	532
BcnI	CCSGG	9 cut(s)	249, 250, 923, 1306, 1307, 1657, 1796, 1823, 1865
BcoDI	GTCTC	3 cut(s)	644, 1295, 1722
BfaI	CTAG	4 cut(s)	929, 1266, 1607, 1883
BfuAI	ACCTGC	1 cut(s)	992
Bme18I	GGWCC	3 cut(s)	304, 344, 981
BmeT110I	CYCGRG	2 cut(s)	248, 1305
BmgT120I	GGNCC	4 cut(s)	304, 344, 981, 1183
BmiI	GGNNCC	6 cut(s)	26, 306, 1316, 1356, 1761, 1820
BoxI	GACNNNNGTC	1 cut(s)	636
BpiI	GAAGAC	2 cut(s)	1254, 1506
BpmI	CTGGAG	3 cut(s)	405, 1304, 1362
Bpu10I	CCTNAGC	1 cut(s)	824
BpuMI	CCSGG	9 cut(s)	249, 250, 923, 1306, 1307, 1657, 1796, 1823, 1865
BsaBI	GATNNNNATC	1 cut(s)	365
BsaI	GGTCTC	3 cut(s)	644, 1295, 1722
BsaJI	CCNNGG	6 cut(s)	248, 679, 1008, 1281, 1305, 1655
BsaWI	WCCGGW	3 cut(s)	53, 301, 1762
BsaXI	ACNNNNNCTCC	4 cut(s)	378, 408, 1452, 1482
Bse118I	RCCGGY	4 cut(s)	53, 301, 610, 1762
Bse1I	ACTGG	5 cut(s)	622, 1049, 1318, 1345, 1418
Bse21I	CCTNAGG	1 cut(s)	582
Bse3DI	GCAATG	1 cut(s)	1595
Bse8I	GATNNNNATC	1 cut(s)	365
BseBI	CCWGG	5 cut(s)	508, 893, 1140, 1283, 1325
BseDI	CCNNGG	6 cut(s)	248, 679, 1008, 1281, 1305, 1655
BseGI	GGATG	7 cut(s)	484, 811, 1135, 1229, 1450, 1660, 1941
BseJI	GATNNNNATC	1 cut(s)	365
BseMI	GCAATG	1 cut(s)	1595
BseMII	CTCAG	4 cut(s)	122, 573, 1040, 1383
BseNI	ACTGG	5 cut(s)	622, 1049, 1318, 1345, 1418
BseRI	GAGGAG	4 cut(s)	238, 962, 970, 1284
BseSI	GKGCMC	1 cut(s)	301
BseX3I	CGGCCG	1 cut(s)	1692
BseXI	GCAGC	7 cut(s)	674, 1034, 1076, 1142, 1340, 1369, 1732
BseYI	CCCAGC	2 cut(s)	432, 1150
BsgI	GTGCAG	1 cut(s)	15
Bsh1236I	CGCG	3 cut(s)	996, 1757, 1920
Bsh1285I	CGRYCG	1 cut(s)	1695
BshFI	GGCC	6 cut(s)	453, 506, 1185, 1264, 1694, 1863
BshNI	GGYRCC	3 cut(s)	24, 1354, 1759
BshTI	ACCGGT	3 cut(s)	53, 301, 1762
BsiEI	CGRYCG	1 cut(s)	1695
BsiHKAI	GWGCWC	1 cut(s)	301
BsiHKCI	CYCGRG	2 cut(s)	248, 1305
BsiWI	CGTACG	1 cut(s)	18
BslFI	GGGAC	2 cut(s)	114, 290
BsmAI	GTCTC	3 cut(s)	644, 1295, 1722
BsmFI	GGGAC	2 cut(s)	114, 290
BsmI	GAATGC	1 cut(s)	665
BsnI	GGCC	6 cut(s)	453, 506, 1185, 1264, 1694, 1863
Bso31I	GGTCTC	3 cut(s)	644, 1295, 1722
BsoBI	CYCGRG	2 cut(s)	248, 1305
Bsp1286I	GDGCHC	2 cut(s)	301, 1836
Bsp1407I	TGTACA	1 cut(s)	1557
Bsp143I	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
Bsp19I	CCATGG	1 cut(s)	1008
BspANI	GGCC	6 cut(s)	453, 506, 1185, 1264, 1694, 1863
BspCNI	CTCAG	4 cut(s)	123, 574, 1039, 1384
BspFNI	CGCG	3 cut(s)	996, 1757, 1920
BspHI	TCATGA	1 cut(s)	951
BspLI	GGNNCC	6 cut(s)	26, 306, 1316, 1356, 1761, 1820
BspMI	ACCTGC	1 cut(s)	992
BspPI	GGATC	8 cut(s)	837, 1040, 1309, 1322, 1430, 1813, 1826, 1863
BspT107I	GGYRCC	3 cut(s)	24, 1354, 1759
BspTNI	GGTCTC	3 cut(s)	644, 1295, 1722
BsrBI	CCGCTC	1 cut(s)	1776
BsrDI	GCAATG	1 cut(s)	1595
BsrFI	RCCGGY	4 cut(s)	53, 301, 610, 1762
BsrGI	TGTACA	1 cut(s)	1557
BsrI	ACTGG	5 cut(s)	622, 1049, 1318, 1345, 1418
BssAI	RCCGGY	4 cut(s)	53, 301, 610, 1762
BssECI	CCNNGG	6 cut(s)	248, 679, 1008, 1281, 1305, 1655
BssMI	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
BssSI	CACGAG	1 cut(s)	1673
BssT1I	CCWWGG	2 cut(s)	679, 1008
Bst2BI	CACGAG	1 cut(s)	1673
Bst2UI	CCWGG	5 cut(s)	508, 893, 1140, 1283, 1325
Bst4CI	ACNGT	3 cut(s)	105, 769, 851
BstAPI	GCANNNNNTGC	1 cut(s)	1766
BstAUI	TGTACA	1 cut(s)	1557
BstC8I	GCNNGC	6 cut(s)	612, 1156, 1348, 1570, 1716, 1889
BstDEI	CTNAG	8 cut(s)	131, 219, 582, 824, 1026, 1094, 1392, 1665
BstDSI	CCRYGG	1 cut(s)	1008
BstENI	CCTNNNNNAGG	1 cut(s)	988
BstF5I	GGATG	7 cut(s)	484, 811, 1135, 1229, 1450, 1660, 1941
BstFNI	CGCG	3 cut(s)	996, 1757, 1920
BstHHI	GCGC	7 cut(s)	996, 1720, 1811, 1882, 1910, 1920, 1941
BstKTI	GATC	9 cut(s)	325, 535, 832, 970, 1048, 1317, 1425, 1821, 1858
BstMAI	GTCTC	3 cut(s)	644, 1295, 1722
BstMBI	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
BstMCI	CGRYCG	1 cut(s)	1695
BstNI	CCWGG	5 cut(s)	508, 893, 1140, 1283, 1325
BstPAI	GACNNNNGTC	1 cut(s)	636
BstSLI	GKGCMC	1 cut(s)	301
BstUI	CGCG	3 cut(s)	996, 1757, 1920
BstV1I	GCAGC	7 cut(s)	674, 1034, 1076, 1142, 1340, 1369, 1732
BstV2I	GAAGAC	2 cut(s)	1254, 1506
BstX2I	RGATCY	3 cut(s)	1314, 1818, 1855
BstXI	CCANNNNNNTGG	1 cut(s)	570
BstYI	RGATCY	3 cut(s)	1314, 1818, 1855
BstZI	CGGCCG	1 cut(s)	1692
Bsu36I	CCTNAGG	1 cut(s)	582
BsuRI	GGCC	6 cut(s)	453, 506, 1185, 1264, 1694, 1863
BtgI	CCRYGG	1 cut(s)	1008
BtsCI	GGATG	7 cut(s)	484, 811, 1135, 1229, 1450, 1660, 1941
BtsI	GCAGTG	1 cut(s)	1424
BtsIMutI	CAGTG	2 cut(s)	765, 1424
BveI	ACCTGC	1 cut(s)	992
Cac8I	GCNNGC	6 cut(s)	612, 1156, 1348, 1570, 1716, 1889
CaiI	CAGNNNCTG	3 cut(s)	347, 984, 1324
CciI	TCATGA	1 cut(s)	951
CfoI	GCGC	7 cut(s)	996, 1720, 1811, 1882, 1910, 1920, 1941
Cfr10I	RCCGGY	4 cut(s)	53, 301, 610, 1762
Cfr13I	GGNCC	4 cut(s)	304, 344, 981, 1183
Cfr9I	CCCGGG	2 cut(s)	248, 1305
CseI	GACGC	2 cut(s)	548, 1763
Csp6I	GTAC	5 cut(s)	19, 25, 229, 1321, 1558
CspAI	ACCGGT	3 cut(s)	53, 301, 1762
CspCI	CAANNNNNGTGG	2 cut(s)	220, 255
CviQI	GTAC	5 cut(s)	19, 25, 229, 1321, 1558
DdeI	CTNAG	8 cut(s)	131, 219, 582, 824, 1026, 1094, 1392, 1665
DpnI	GATC	9 cut(s)	324, 534, 831, 969, 1047, 1316, 1424, 1820, 1857
DpnII	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
EaeI	YGGCCR	2 cut(s)	1692, 1861
EagI	CGGCCG	1 cut(s)	1692
EciI	GGCGGA	1 cut(s)	659
EclXI	CGGCCG	1 cut(s)	1692
Eco130I	CCWWGG	2 cut(s)	679, 1008
Eco147I	AGGCCT	1 cut(s)	1264
Eco24I	GRGCYC	1 cut(s)	1836
Eco31I	GGTCTC	3 cut(s)	644, 1295, 1722
Eco47I	GGWCC	3 cut(s)	304, 344, 981
Eco52I	CGGCCG	1 cut(s)	1692
Eco81I	CCTNAGG	1 cut(s)	582
Eco88I	CYCGRG	2 cut(s)	248, 1305
EcoNI	CCTNNNNNAGG	1 cut(s)	988
EcoO109I	RGGNCCY	2 cut(s)	344, 981
EcoRII	CCWGG	5 cut(s)	506, 891, 1138, 1281, 1323
EcoT14I	CCWWGG	2 cut(s)	679, 1008
EcoT22I	ATGCAT	2 cut(s)	199, 1231
EcoT38I	GRGCYC	1 cut(s)	1836
ErhI	CCWWGG	2 cut(s)	679, 1008
FalI	AAGNNNNNCTT	2 cut(s)	405, 437
FaqI	GGGAC	2 cut(s)	114, 290
FauI	CCCGC	3 cut(s)	268, 1762, 1880
FbaI	TGATCA	1 cut(s)	532
FblI	GTMKAC	2 cut(s)	244, 519
FokI	GGATG	7 cut(s)	491, 818, 1122, 1216, 1457, 1647, 1928
FriOI	GRGCYC	1 cut(s)	1836
FspBI	CTAG	4 cut(s)	929, 1266, 1607, 1883
GlaI	GCGC	7 cut(s)	995, 1719, 1810, 1881, 1909, 1919, 1940
GsaI	CCCAGC	2 cut(s)	436, 1154
GsuI	CTGGAG	3 cut(s)	405, 1304, 1362
HaeIII	GGCC	6 cut(s)	453, 506, 1185, 1264, 1694, 1863
HgaI	GACGC	2 cut(s)	548, 1763
HhaI	GCGC	7 cut(s)	996, 1720, 1811, 1882, 1910, 1920, 1941
Hin6I	GCGC	7 cut(s)	994, 1718, 1809, 1880, 1908, 1918, 1939
HinP1I	GCGC	7 cut(s)	994, 1718, 1809, 1880, 1908, 1918, 1939
HindIII	AAGCTT	1 cut(s)	548
HphI	GGTGA	7 cut(s)	417, 617, 695, 890, 1090, 1675, 1856
Hpy166II	GTNNAC	7 cut(s)	245, 299, 460, 520, 631, 1003, 1581
Hpy8I	GTNNAC	7 cut(s)	245, 299, 460, 520, 631, 1003, 1581
Hpy99I	CGWCG	1 cut(s)	43
HpyCH4III	ACNGT	3 cut(s)	105, 769, 851
HpyF3I	CTNAG	8 cut(s)	131, 219, 582, 824, 1026, 1094, 1392, 1665
HspAI	GCGC	7 cut(s)	994, 1718, 1809, 1880, 1908, 1918, 1939
KpnI	GGTACC	1 cut(s)	28
KroI	GCCGGC	1 cut(s)	610
KroNI	GCCGGC	1 cut(s)	612
Ksp22I	TGATCA	1 cut(s)	532
Kzo9I	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
LmnI	GCTCC	5 cut(s)	386, 483, 1343, 1477, 1773
Lsp1109I	GCAGC	7 cut(s)	674, 1034, 1076, 1142, 1340, 1369, 1732
MaeI	CTAG	4 cut(s)	929, 1266, 1607, 1883
MaeIII	GTNAC	2 cut(s)	959, 1681
MalI	GATC	9 cut(s)	324, 534, 831, 969, 1047, 1316, 1424, 1820, 1857
MbiI	CCGCTC	1 cut(s)	1776
MboI	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
MflI	RGATCY	3 cut(s)	1314, 1818, 1855
MhlI	GDGCHC	2 cut(s)	301, 1836
MluCI	AATT	5 cut(s)	93, 379, 706, 1035, 1541
MlyI	GAGTC	5 cut(s)	525, 1281, 1615, 1689, 1697
MmeI	TCCRAC	2 cut(s)	1422, 1683
Mph1103I	ATGCAT	2 cut(s)	199, 1231
MroNI	GCCGGC	1 cut(s)	610
MroXI	GAANNNNTTC	1 cut(s)	783
MseI	TTAA	2 cut(s)	189, 1545
MslI	CAYNNNNRTG	3 cut(s)	202, 657, 1307
MspA1I	CMGCKG	2 cut(s)	791, 1391
Mva1269I	GAATGC	1 cut(s)	665
MvaI	CCWGG	5 cut(s)	508, 893, 1140, 1283, 1325
MvnI	CGCG	3 cut(s)	996, 1757, 1920
NaeI	GCCGGC	1 cut(s)	612
NciI	CCSGG	9 cut(s)	249, 250, 923, 1306, 1307, 1657, 1796, 1823, 1865
NcoI	CCATGG	1 cut(s)	1008
NdeII	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
NgoMIV	GCCGGC	1 cut(s)	610
NlaIV	GGNNCC	6 cut(s)	26, 306, 1316, 1356, 1761, 1820
NmeAIII	GCCGAG	2 cut(s)	1627, 1723
NmuCI	GTSAC	1 cut(s)	1681
NsiI	ATGCAT	2 cut(s)	199, 1231
OliI	CACNNNNGTG	1 cut(s)	1307
PagI	TCATGA	1 cut(s)	951
PceI	AGGCCT	1 cut(s)	1264
PcsI	WCGNNNNNNNCGW	1 cut(s)	78
PctI	GAATGC	1 cut(s)	665
PdiI	GCCGGC	1 cut(s)	612
PdmI	GAANNNNTTC	1 cut(s)	783
PfeI	GAWTC	5 cut(s)	127, 439, 940, 1030, 1669
Pfl23II	CGTACG	1 cut(s)	18
PflMI	CCANNNNNTGG	2 cut(s)	1009, 1324
PfoI	TCCNGGA	2 cut(s)	1138, 1821
PinAI	ACCGGT	3 cut(s)	53, 301, 1762
PleI	GAGTC	5 cut(s)	524, 1280, 1615, 1688, 1696
PpsI	GAGTC	5 cut(s)	524, 1280, 1615, 1688, 1696
PpuMI	RGGWCCY	2 cut(s)	344, 981
PshAI	GACNNNNGTC	1 cut(s)	636
Psp5II	RGGWCCY	2 cut(s)	344, 981
Psp6I	CCWGG	5 cut(s)	506, 891, 1138, 1281, 1323
PspFI	CCCAGC	2 cut(s)	432, 1150
PspGI	CCWGG	5 cut(s)	506, 891, 1138, 1281, 1323
PspLI	CGTACG	1 cut(s)	18
PspN4I	GGNNCC	6 cut(s)	26, 306, 1316, 1356, 1761, 1820
PspPI	GGNCC	4 cut(s)	304, 344, 981, 1183
PspPPI	RGGWCCY	2 cut(s)	344, 981
PsrI	GAACNNNNNNTAC	2 cut(s)	503, 535
PstNI	CAGNNNCTG	3 cut(s)	347, 984, 1324
PsuI	RGATCY	3 cut(s)	1314, 1818, 1855
PvuII	CAGCTG	2 cut(s)	791, 1391
RsaI	GTAC	5 cut(s)	20, 26, 230, 1322, 1559
RsaNI	GTAC	5 cut(s)	19, 25, 229, 1321, 1558
RseI	CAYNNNNRTG	3 cut(s)	202, 657, 1307
SaqAI	TTAA	2 cut(s)	189, 1545
Sau3AI	GATC	9 cut(s)	322, 532, 829, 967, 1045, 1314, 1422, 1818, 1855
Sau96I	GGNCC	4 cut(s)	304, 344, 981, 1183
SchI	GAGTC	5 cut(s)	525, 1281, 1615, 1689, 1697
SduI	GDGCHC	2 cut(s)	301, 1836
SgrAI	CRCCGGYG	1 cut(s)	53
SinI	GGWCC	3 cut(s)	304, 344, 981
SmaI	CCCGGG	2 cut(s)	250, 1307
SmiMI	CAYNNNNRTG	3 cut(s)	202, 657, 1307
Sse9I	AATT	5 cut(s)	93, 379, 706, 1035, 1541
SseBI	AGGCCT	1 cut(s)	1264
SspMI	CTAG	4 cut(s)	929, 1266, 1607, 1883
StuI	AGGCCT	1 cut(s)	1264
StyI	CCWWGG	2 cut(s)	679, 1008
TaaI	ACNGT	3 cut(s)	105, 769, 851
TaqI	TCGA	2 cut(s)	717, 1626
TasI	AATT	5 cut(s)	93, 379, 706, 1035, 1541
TatI	WGTACW	1 cut(s)	1557
TauI	GCSGC	4 cut(s)	675, 1697, 1760, 1913
TfiI	GAWTC	5 cut(s)	127, 439, 940, 1030, 1669
Tru1I	TTAA	2 cut(s)	189, 1545
Tru9I	TTAA	2 cut(s)	189, 1545
TscAI	CASTG	2 cut(s)	772, 1424
TseFI	GTSAC	1 cut(s)	1681
TseI	GCWGC	7 cut(s)	662, 1022, 1064, 1130, 1328, 1382, 1720
Tsp45I	GTSAC	1 cut(s)	1681
TspDTI	ATGAA	7 cut(s)	62, 262, 388, 822, 940, 953, 1074
TspMI	CCCGGG	2 cut(s)	248, 1305
TspRI	CASTG	2 cut(s)	772, 1424
Van91I	CCANNNNNTGG	2 cut(s)	1009, 1324
VneI	GTGCAC	1 cut(s)	297
VpaK11BI	GGWCC	3 cut(s)	304, 344, 981
XagI	CCTNNNNNAGG	1 cut(s)	988
XapI	RAATTY	2 cut(s)	93, 1541
XbaI	TCTAGA	1 cut(s)	1606
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1015
XmaI	CCCGGG	2 cut(s)	248, 1305
XmiI	GTMKAC	2 cut(s)	244, 519
XmnI	GAANNNNTTC	1 cut(s)	783
XspI	CTAG	4 cut(s)	929, 1266, 1607, 1883
Zsp2I	ATGCAT	2 cut(s)	199, 1231

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.