AT5G05230

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006755
Physical Location & Seq
Forward (+)
1551298 .. 1553808
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05230.1

Sequence Viewer

Length: 1092 bp
ATGTCCGGCAAAGGTCTATCTCCGGCGCCGTTCAACGCGCAGCCGATGATTGTCCAAGATGCTGACCCGTTACGTTTTCGGGTCGGCGAACAGGATCCTAAAACCCGTGAATTCGCTGCCTTTATCGGCGACCACCGGTATTTCGCGGCAGCAGCAGCAGCAGCCGCCGCAGCTAATCCACATCCTCATCTCGAGTTCCGTCAGAATTTCTACTCGGAGAAACCCATTATCGGAAACCCTAACGACAGTGGTGGATCCGACGGCGAGGATGACGTTGACGTGGAAGAAGAAGACGAGGATGATGACTTAGACGGAAACGAAGGTGACATCGGCATGAACAAAGACGCTGGTGAAGATTCTGTATCTGCAGGAGCTGTGATAGTGGTTGGACAAGACAACGCAGCTTACTATTCCCAGCATTTTAAGACAGTGGAAGCGAGTTTTGTTTCTAGGAATGAGGAATCATCAATCGCTGCGGATAATGGGTGTGATTTCAGTGGCAGAAGAGACCTTTCTTCTTCTTCGAGCAATTCGATCGAGTCATTGAGAACCATTTTGTCTGATCCTACAACAGGCTCTTTGATGGCTGATGCTATGATTTTACCTTGCGGACACACTTTTGGAGCTGGAGGGATAGAACAAGTTAAACAGATGAAAGCTTGTTGCACATGTTCACAACCAGTCTCAGAAGACTCGATAACCCCAAATCTCACTTTACGAGTTGCGGTACAAGCATTCTGCCGAGAAGAGAATTCACAGTCAAACCATCCATCTAAGAGAAAACGAGAAGGATTTGATCAAGAGAGACGCGCATTTGGTGTCACGAATCATTCAGGTCGTTCAAGAAACAAAAGTAACCATTTCCCATTTGCTGTGGCAGATCGAGTTATAATAAAGGGAAATAAGAGGACACCACCTCGTTTTGTTGGCCGGGAAGCTGTTGTGACTACTCAATGCTTAAATGGCTGGTATGTGGTGAAAACACTGGACAACGCAGAGAGTGTGAAGCTTCAGTATCGTTCATTGGCTAAAGCTCCAGAAGATCCATCAGCTAAAGCAACACCAAACAAGATGGTCTCTAACTGGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

363

Amino Acids

39.37

Weight (kDa)

5.15

Isoelectric Point (pI)

54.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB62-63_C PF23112 287 - 344 2.2e-33 U-box protein 62/63 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
AccB1I GGYRCC 1 cut(s) 25
AccII CGCG 3 cut(s) 38, 146, 810
AciI CCGC 6 cut(s) 146, 165, 168, 476, 609, 725
AclWI GGATC 6 cut(s) 89, 102, 249, 262, 557, 1037
AcoI YGGCCR 1 cut(s) 928
AcsI RAATTY 3 cut(s) 110, 205, 751
AcuI CTGAAG 1 cut(s) 995
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 1 cut(s) 729
AfiI CCNNNNNNNGG 2 cut(s) 230, 572
AflIII ACRYGT 1 cut(s) 668
AgeI ACCGGT 1 cut(s) 135
AgsI TTSAA 2 cut(s) 34, 843
AjiI CACGTC 1 cut(s) 280
AluBI AGCT 9 cut(s) 173, 374, 404, 626, 659, 938, 1009, 1034, 1052
AluI AGCT 9 cut(s) 173, 374, 404, 626, 659, 938, 1009, 1034, 1052
Alw26I GTCTC 4 cut(s) 501, 688, 799, 1081
AlwI GGATC 6 cut(s) 89, 102, 249, 262, 557, 1037
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 1 cut(s) 928
ApoI RAATTY 3 cut(s) 110, 205, 751
AsiGI ACCGGT 1 cut(s) 135
AspLEI GCGC 3 cut(s) 28, 40, 812
AsuC2I CCSGG 1 cut(s) 932
AsuHPI GGTGA 3 cut(s) 335, 362, 988
AvaI CYCGRG 1 cut(s) 191
BamHI GGATCC 2 cut(s) 94, 254
BanI GGYRCC 1 cut(s) 25
BbsI GAAGAC 2 cut(s) 297, 696
BccI CCATC 5 cut(s) 577, 774, 778, 1054, 1066
BceAI ACGGC 2 cut(s) 13, 277
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 1 cut(s) 932
BcoDI GTCTC 4 cut(s) 501, 688, 799, 1081
BfaI CTAG 1 cut(s) 450
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 932
BmeT110I CYCGRG 1 cut(s) 191
BmgBI CACGTC 1 cut(s) 280
BmiI GGNNCC 3 cut(s) 27, 96, 256
BmrFI CCNGG 1 cut(s) 932
BmsI GCATC 2 cut(s) 49, 580
BpiI GAAGAC 2 cut(s) 297, 696
BpmI CTGGAG 2 cut(s) 648, 1020
BpuMI CCSGG 1 cut(s) 932
BsaHI GRCGYC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 501, 1081
BsaWI WCCGGW 1 cut(s) 135
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 572
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 3 cut(s) 680, 990, 1088
BseGI GGATG 4 cut(s) 181, 274, 304, 766
BseLI CCNNNNNNNGG 2 cut(s) 230, 572
BseMII CTCAG 1 cut(s) 699
BseNI ACTGG 3 cut(s) 680, 990, 1088
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 3 cut(s) 38, 146, 810
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 1 cut(s) 930
BshNI GGYRCC 1 cut(s) 25
BshTI ACCGGT 1 cut(s) 135
BsiEI CGRYCG 1 cut(s) 537
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 4 cut(s) 6, 23, 136, 931
BslI CCNNNNNNNGG 2 cut(s) 230, 572
BsmAI GTCTC 4 cut(s) 501, 688, 799, 1081
BsmBI CGTCTC 1 cut(s) 799
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 1 cut(s) 930
Bso31I GGTCTC 2 cut(s) 501, 1081
BsoBI CYCGRG 1 cut(s) 191
Bsp143I GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
BspACI CCGC 6 cut(s) 146, 165, 168, 476, 609, 725
BspANI GGCC 1 cut(s) 930
BspCNI CTCAG 1 cut(s) 698
BspFNI CGCG 3 cut(s) 38, 146, 810
BspLI GGNNCC 3 cut(s) 27, 96, 256
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 6 cut(s) 89, 102, 249, 262, 557, 1037
BspT107I GGYRCC 1 cut(s) 25
BspTNI GGTCTC 2 cut(s) 501, 1081
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 3 cut(s) 680, 990, 1088
BssAI RCCGGY 1 cut(s) 135
BssMI GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
BssNI GRCGYC 1 cut(s) 26
Bst4CI ACNGT 3 cut(s) 248, 430, 759
Bst6I CTCTTC 2 cut(s) 499, 741
BstACI GRCGYC 1 cut(s) 26
BstDEI CTNAG 3 cut(s) 307, 685, 774
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 4 cut(s) 181, 274, 304, 766
BstFNI CGCG 3 cut(s) 38, 146, 810
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 3 cut(s) 28, 40, 812
BstKTI GATC 7 cut(s) 97, 257, 537, 565, 799, 883, 1045
BstMAI GTCTC 4 cut(s) 501, 688, 799, 1081
BstMBI GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
BstMCI CGRYCG 1 cut(s) 537
BstMWI GCNNNNNNNGC 9 cut(s) 152, 155, 158, 161, 164, 167, 170, 731, 963
BstNSI RCATGY 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 930
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 3 cut(s) 38, 146, 810
BstV2I GAAGAC 2 cut(s) 297, 696
BstX2I RGATCY 3 cut(s) 94, 254, 1042
BstYI RGATCY 3 cut(s) 94, 254, 1042
BsuRI GGCC 1 cut(s) 930
BtrI CACGTC 1 cut(s) 280
BtsCI GGATG 4 cut(s) 181, 274, 304, 766
BtsIMutI CAGTG 4 cut(s) 253, 435, 502, 983
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 3 cut(s) 28, 40, 812
Cfr10I RCCGGY 1 cut(s) 135
CseI GACGC 2 cut(s) 353, 816
Csp6I GTAC 1 cut(s) 728
CspAI ACCGGT 1 cut(s) 135
CviAII CATG 2 cut(s) 334, 669
CviQI GTAC 1 cut(s) 728
DdeI CTNAG 3 cut(s) 307, 685, 774
DinI GGCGCC 1 cut(s) 27
DpnI GATC 7 cut(s) 96, 256, 536, 564, 798, 882, 1044
DpnII GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
EaeI YGGCCR 1 cut(s) 928
Eam1104I CTCTTC 2 cut(s) 499, 741
EarI CTCTTC 2 cut(s) 499, 741
Eco31I GGTCTC 2 cut(s) 501, 1081
Eco57I CTGAAG 1 cut(s) 995
Eco88I CYCGRG 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoRI GAATTC 2 cut(s) 110, 751
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 799
FaeI CATG 2 cut(s) 337, 672
FaiI YATR 5 cut(s) 335, 596, 670, 890, 972
FatI CATG 2 cut(s) 333, 668
FbaI TGATCA 1 cut(s) 796
FokI GGATG 4 cut(s) 168, 281, 311, 753
FspBI CTAG 1 cut(s) 450
GlaI GCGC 3 cut(s) 27, 39, 811
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 2 cut(s) 648, 1020
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 1 cut(s) 930
HapII CCGG 4 cut(s) 6, 23, 136, 931
HgaI GACGC 2 cut(s) 353, 816
HhaI GCGC 3 cut(s) 28, 40, 812
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 2 cut(s) 337, 672
Hin6I GCGC 3 cut(s) 26, 38, 810
HinP1I GCGC 3 cut(s) 26, 38, 810
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HindIII AAGCTT 2 cut(s) 657, 1007
HinfI GANTC 5 cut(s) 356, 461, 539, 692, 826
HpaII CCGG 4 cut(s) 6, 23, 136, 931
HphI GGTGA 3 cut(s) 335, 362, 988
Hpy166II GTNNAC 2 cut(s) 277, 674
Hpy188I TCNGA 6 cut(s) 204, 217, 233, 259, 562, 688
Hpy188III TCNNGA 5 cut(s) 191, 800, 823, 843, 1037
Hpy8I GTNNAC 2 cut(s) 277, 674
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 314, 782
HpyCH4III ACNGT 3 cut(s) 248, 430, 759
HpyCH4IV ACGT 3 cut(s) 73, 273, 279
HpyCH4V TGCA 2 cut(s) 368, 666
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 155, 158, 161, 164, 167, 170, 731, 963
HpyF3I CTNAG 3 cut(s) 307, 685, 774
HpySE526I ACGT 3 cut(s) 73, 273, 279
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 2 cut(s) 337, 672
HspAI GCGC 3 cut(s) 26, 38, 810
KasI GGCGCC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
LmnI GCTCC 3 cut(s) 371, 623, 1039
LweI GCATC 2 cut(s) 49, 580
MaeI CTAG 1 cut(s) 450
MaeII ACGT 3 cut(s) 73, 273, 279
MaeIII GTNAC 5 cut(s) 69, 323, 820, 854, 943
MalI GATC 7 cut(s) 96, 256, 536, 564, 798, 882, 1044
MboI GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
MflI RGATCY 3 cut(s) 94, 254, 1042
MluCI AATT 4 cut(s) 110, 205, 529, 751
Mly113I GGCGCC 1 cut(s) 26
MlyI GAGTC 2 cut(s) 548, 686
MmeI TCCRAC 2 cut(s) 282, 367
MnlI CCTC 7 cut(s) 195, 259, 289, 451, 623, 900, 927
MseI TTAA 3 cut(s) 423, 645, 959
MslI CAYNNNNRTG 1 cut(s) 332
MspI CCGG 4 cut(s) 6, 23, 136, 931
MspR9I CCNGG 1 cut(s) 932
Mva1269I GAATGC 1 cut(s) 734
MvnI CGCG 3 cut(s) 38, 146, 810
MwoI GCNNNNNNNGC 9 cut(s) 152, 155, 158, 161, 164, 167, 170, 731, 963
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 1 cut(s) 932
NdeII GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
NlaIII CATG 2 cut(s) 337, 672
NlaIV GGNNCC 3 cut(s) 27, 96, 256
NmeAIII GCCGAG 1 cut(s) 767
NmuCI GTSAC 3 cut(s) 323, 820, 943
NspI RCATGY 1 cut(s) 672
PaeR7I CTCGAG 1 cut(s) 191
PciI ACATGT 1 cut(s) 668
PcsI WCGNNNNNNNCGW 1 cut(s) 530
PctI GAATGC 1 cut(s) 734
PfeI GAWTC 3 cut(s) 356, 461, 826
PinAI ACCGGT 1 cut(s) 135
Ple19I CGATCG 1 cut(s) 537
PleI GAGTC 2 cut(s) 547, 686
PluTI GGCGCC 1 cut(s) 29
PpsI GAGTC 2 cut(s) 547, 686
PscI ACATGT 1 cut(s) 668
PsiI TTATAA 1 cut(s) 890
PspFI CCCAGC 1 cut(s) 414
PspN4I GGNNCC 3 cut(s) 27, 96, 256
PstI CTGCAG 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 3 cut(s) 94, 254, 1042
PvuI CGATCG 1 cut(s) 537
RsaI GTAC 1 cut(s) 729
RsaNI GTAC 1 cut(s) 728
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 3 cut(s) 423, 645, 959
Sau3AI GATC 7 cut(s) 94, 254, 534, 562, 796, 880, 1042
SchI GAGTC 2 cut(s) 548, 686
ScrFI CCNGG 1 cut(s) 932
SfaNI GCATC 2 cut(s) 49, 580
SfcI CTRYAG 1 cut(s) 366
SfoI GGCGCC 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 4 cut(s) 110, 205, 529, 751
SsiI CCGC 6 cut(s) 146, 165, 168, 476, 609, 725
SspDI GGCGCC 1 cut(s) 25
SspMI CTAG 1 cut(s) 450
StyD4I CCNGG 1 cut(s) 930
TaaI ACNGT 3 cut(s) 248, 430, 759
TaiI ACGT 3 cut(s) 76, 276, 282
TaqI TCGA 6 cut(s) 192, 524, 533, 537, 695, 883
TasI AATT 4 cut(s) 110, 205, 529, 751
TauI GCSGC 3 cut(s) 149, 167, 170
TfiI GAWTC 3 cut(s) 356, 461, 826
Tru1I TTAA 3 cut(s) 423, 645, 959
Tru9I TTAA 3 cut(s) 423, 645, 959
TscAI CASTG 4 cut(s) 253, 435, 502, 990
TseFI GTSAC 3 cut(s) 323, 820, 943
Tsp45I GTSAC 3 cut(s) 323, 820, 943
TspDTI ATGAA 3 cut(s) 350, 668, 1011
TspGWI ACGGA 2 cut(s) 188, 327
TspRI CASTG 4 cut(s) 253, 435, 502, 990
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 3 cut(s) 110, 205, 751
XceI RCATGY 1 cut(s) 672
XhoI CTCGAG 1 cut(s) 191
XspI CTAG 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.