Prupe.2G264200_v2.0.a1

U-box domain-containing protein 62-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0006755
Physical Location & Seq
Reverse (-)
27178165 .. 27181906
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G264200.1

Sequence Viewer

Length: 1335 bp
ATGTCCTCCGAAAAATTAGGTCTGGTTCCGACCCAGAACGGGCTCAACTCTCAGCTCGTTTTCCAAGACGAGAACTTGCGCTTCAATTGCGGAGCTTCGCCTCAGCGCCGGGTCGGGGACTCGGGTCCGAAGACCCGAGAGCTCAGCGGCTTCATTGACGACCGGTTTTTCGCGCCGCAAAGCGCTGATTTCCGCCCCAGCATGTATAACGAGAATCCGGACCGCCGGGAGCCACCCGAACCCCGAAACTGGAACTCGAATGGAGCCGACACGACGCCGAGCGGTGAGGGATCGGACGAGGATGACGACGACGACGACGAGGAGGATGACGTGGACGATGATGATGAGGTTGATGAAGGTGACGCTGAAGTTGGAGGGCTTGTCGGAGTGGATAACAGAAACAAGGGCATTGCTAGTAATAATGGGAACAATGGGGAAGGCAAAATGGGAAATGGGAAAGTTAAGCACCATAACCAGCACCATTCTTCTTTTGGTGGAACTGTGATAGTAGGATCAAGTAGAGAGGTGTTGGTGAAAGACCATAGTGTGGGGCAGCAAATAATGAACAACAACACAAGGGCTAGTCCTAGTGATACTCAGCAAGGAAGGCTTGGAAACTATCACAATGTGGTCACAGTCGCAGAACCAGACGGTGACATTTATTATTCTCAGTATTTGCAGGGGTCTGAGGGGTCTGGTTCTGCTCAAAAGGATTCAGTTGTGGAAAATGGATGTGGGTTTAGTGGAAGAAAGGATGTTATGTATTCGTCCGAGTCTGGAGACTCTTTGAGAGCAATTCTCTCTGATCCTGTGACGGGAGCTCTGATGGATGATGCAATGATAATGCCTTGCGGGCATTCCTTTGGAGGTAGTGGAATACAGCATGTTATTAGAATGAAAGCTTGCTACACCTGTTCTCAGTCAATTTCAGAAGATTCAATTGCCCCAAATCTCTCTCTTCGAGCTGCTGTGCTGGCATTCCGCCGGGAAGAAGAGTTACAATTTTATCGCTCATCCAAAAGAAGAAGAGAAAGGTTTGACCAGGAAAAGGGTGGTTACAGTGATTCAGTTCTCGTGGACACTCCGAGGGGTAGAGGTGTTCAGTTTCCATTTGTAGTGACAGACCGAGTTATTATAAAGGGGAATAAGAGGACACCGCAGCGCTTTGTGGGGCGCGAGGCTGTTGTCACAACACAATGCTTAAATGGATGGTATGTGGTAAAGACATTGGATAATGCAGAGAGTGTAAAATTGCAGTACCGGTCACTTGCCAAGGTATCCGATGAACCCTCATCTAAGCCAATGTCAAGCAAGATGGGACCTAACTGGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

445

Amino Acids

48.64

Weight (kDa)

4.97

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1136
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 282
AccII CGCG 2 cut(s) 173, 1176
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 9 cut(s) 90, 147, 176, 193, 223, 282, 852, 982, 1157
AclWI GGATC 3 cut(s) 298, 520, 800
AcuI CTGAAG 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 628
AfaI GTAC 1 cut(s) 1259
AfeI AGCGCT 2 cut(s) 184, 1163
AfiI CCNNNNNNNGG 6 cut(s) 39, 115, 249, 547, 815, 1048
AgeI ACCGGT 2 cut(s) 162, 1260
AgsI TTSAA 2 cut(s) 85, 939
AjiI CACGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 1041
AluBI AGCT 6 cut(s) 55, 95, 142, 821, 902, 965
AluI AGCT 6 cut(s) 55, 95, 142, 821, 902, 965
Alw21I GWGCWC 2 cut(s) 144, 823
Alw26I GTCTC 1 cut(s) 774
AlwI GGATC 3 cut(s) 298, 520, 800
Ama87I CYCGRG 2 cut(s) 121, 135
Aor13HI TCCGGA 1 cut(s) 217
Aor51HI AGCGCT 2 cut(s) 184, 1163
ApeKI GCWGC 3 cut(s) 553, 965, 1159
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AsiGI ACCGGT 2 cut(s) 162, 1260
AspLEI GCGC 6 cut(s) 81, 108, 175, 185, 1164, 1176
AspS9I GGNCC 3 cut(s) 125, 220, 1319
AsuC2I CCSGG 3 cut(s) 110, 227, 986
AsuHPI GGTGA 4 cut(s) 296, 371, 544, 665
AvaI CYCGRG 2 cut(s) 121, 135
AvaII GGWCC 3 cut(s) 125, 220, 1319
BanII GRGCYC 3 cut(s) 45, 144, 823
BarI GAAGNNNNNNTAC 2 cut(s) 981, 1013
BauI CACGAG 1 cut(s) 1073
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 144, 823
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 565, 952, 1171
BccI CCATC 3 cut(s) 820, 1203, 1309
BciT130I CCWGG 1 cut(s) 1043
BciVI GTATCC 1 cut(s) 1288
BcnI CCSGG 3 cut(s) 110, 227, 986
BcoDI GTCTC 1 cut(s) 774
BfaI CTAG 4 cut(s) 414, 582, 588, 1333
BfoI RGCGCY 3 cut(s) 109, 186, 1165
BfuI GTATCC 1 cut(s) 1288
BglI GCCNNNNNGGC 1 cut(s) 853
BisI GCNGC 5 cut(s) 148, 176, 554, 966, 1160
BlpI GCTNAGC 1 cut(s) 143
BlsI GCNGC 5 cut(s) 149, 177, 555, 967, 1161
Bme1390I CCNGG 4 cut(s) 110, 227, 986, 1043
Bme18I GGWCC 3 cut(s) 125, 220, 1319
BmeT110I CYCGRG 2 cut(s) 121, 135
BmgBI CACGTC 1 cut(s) 331
BmgT120I GGNCC 3 cut(s) 125, 220, 1319
BmiI GGNNCC 5 cut(s) 27, 126, 231, 265, 1320
BmrFI CCNGG 4 cut(s) 110, 227, 986, 1043
BmsI GCATC 1 cut(s) 823
BoxI GACNNNNGTC 1 cut(s) 123
BpiI GAAGAC 1 cut(s) 137
BplI GAGNNNNNCTC 2 cut(s) 783, 815
BpmI CTGGAG 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 102
Bpu1102I GCTNAGC 1 cut(s) 143
BpuMI CCSGG 3 cut(s) 110, 227, 986
BsaHI GRCGYC 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 1085, 1272
BsaWI WCCGGW 3 cut(s) 162, 217, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 6 cut(s) 39, 115, 249, 547, 815, 1048
Bse118I RCCGGY 2 cut(s) 162, 1260
Bse1I ACTGG 2 cut(s) 254, 1331
Bse3DI GCAATG 2 cut(s) 408, 843
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 2 cut(s) 1085, 1272
BseGI GGATG 7 cut(s) 307, 331, 737, 760, 835, 1013, 1214
BseLI CCNNNNNNNGG 6 cut(s) 39, 115, 249, 547, 815, 1048
BseMI GCAATG 2 cut(s) 408, 843
BseMII CTCAG 7 cut(s) 65, 116, 157, 611, 678, 683, 932
BseNI ACTGG 2 cut(s) 254, 1331
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 3 cut(s) 565, 952, 1171
BseYI CCCAGC 1 cut(s) 197
Bsh1236I CGCG 2 cut(s) 173, 1176
Bsh1285I CGRYCG 1 cut(s) 163
BshTI ACCGGT 2 cut(s) 162, 1260
BsiEI CGRYCG 1 cut(s) 163
BsiHKAI GWGCWC 2 cut(s) 144, 823
BsiHKCI CYCGRG 2 cut(s) 121, 135
BsiSI CCGG 6 cut(s) 109, 163, 218, 226, 985, 1261
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 6 cut(s) 39, 115, 249, 547, 815, 1048
BsmAI GTCTC 1 cut(s) 774
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 2 cut(s) 856, 977
BsoBI CYCGRG 2 cut(s) 121, 135
Bsp1286I GDGCHC 3 cut(s) 45, 144, 823
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 3 cut(s) 290, 512, 805
Bsp1720I GCTNAGC 1 cut(s) 143
BspACI CCGC 9 cut(s) 90, 147, 176, 193, 223, 282, 852, 982, 1157
BspCNI CTCAG 7 cut(s) 64, 115, 156, 610, 679, 682, 931
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 2 cut(s) 173, 1176
BspLI GGNNCC 5 cut(s) 27, 126, 231, 265, 1320
BspPI GGATC 3 cut(s) 298, 520, 800
BsrBI CCGCTC 1 cut(s) 282
BsrDI GCAATG 2 cut(s) 408, 843
BsrFI RCCGGY 2 cut(s) 162, 1260
BsrI ACTGG 2 cut(s) 254, 1331
BssAI RCCGGY 2 cut(s) 162, 1260
BssECI CCNNGG 2 cut(s) 1085, 1272
BssMI GATC 3 cut(s) 290, 512, 805
BssNI GRCGYC 1 cut(s) 275
BssSI CACGAG 1 cut(s) 1073
BssT1I CCWWGG 1 cut(s) 1272
Bst2BI CACGAG 1 cut(s) 1073
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 4 cut(s) 502, 637, 653, 1061
Bst6I CTCTTC 3 cut(s) 963, 987, 1021
BstACI GRCGYC 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 854, 904, 975
BstDEI CTNAG 8 cut(s) 51, 102, 143, 597, 669, 687, 918, 1296
BstF5I GGATG 7 cut(s) 307, 331, 737, 760, 835, 1013, 1214
BstFNI CGCG 2 cut(s) 173, 1176
BstH2I RGCGCY 3 cut(s) 109, 186, 1165
BstHHI GCGC 6 cut(s) 81, 108, 175, 185, 1164, 1176
BstKTI GATC 3 cut(s) 293, 515, 808
BstMAI GTCTC 1 cut(s) 774
BstMBI GATC 3 cut(s) 290, 512, 805
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 4 cut(s) 87, 607, 853, 974
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 2 cut(s) 205, 887
BstPAI GACNNNNGTC 1 cut(s) 123
BstSCI CCNGG 4 cut(s) 108, 225, 984, 1041
BstUI CGCG 2 cut(s) 173, 1176
BstV1I GCAGC 3 cut(s) 565, 952, 1171
BstV2I GAAGAC 1 cut(s) 137
BsuI GTATCC 1 cut(s) 1288
BtrI CACGTC 1 cut(s) 331
BtsCI GGATG 7 cut(s) 307, 331, 737, 760, 835, 1013, 1214
BtsIMutI CAGTG 1 cut(s) 1066
Cac8I GCNNGC 3 cut(s) 854, 904, 975
CfoI GCGC 6 cut(s) 81, 108, 175, 185, 1164, 1176
Cfr10I RCCGGY 2 cut(s) 162, 1260
Cfr13I GGNCC 3 cut(s) 125, 220, 1319
CpoI CGGWCCG 1 cut(s) 220
CseI GACGC 2 cut(s) 283, 371
Csp6I GTAC 1 cut(s) 1258
CspAI ACCGGT 2 cut(s) 162, 1260
CspI CGGWCCG 1 cut(s) 220
CviAII CATG 2 cut(s) 202, 884
CviQI GTAC 1 cut(s) 1258
DdeI CTNAG 8 cut(s) 51, 102, 143, 597, 669, 687, 918, 1296
DpnI GATC 3 cut(s) 292, 514, 807
DpnII GATC 3 cut(s) 290, 512, 805
DraIII CACNNNGTG 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 963, 987, 1021
EarI CTCTTC 3 cut(s) 963, 987, 1021
EciI GGCGGA 2 cut(s) 182, 971
Ecl136II GAGCTC 2 cut(s) 142, 821
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 3 cut(s) 45, 144, 823
Eco47I GGWCC 3 cut(s) 125, 220, 1319
Eco47III AGCGCT 2 cut(s) 184, 1163
Eco53kI GAGCTC 2 cut(s) 142, 821
Eco57I CTGAAG 1 cut(s) 387
Eco88I CYCGRG 2 cut(s) 121, 135
EcoICRI GAGCTC 2 cut(s) 142, 821
EcoO109I RGGNCCY 1 cut(s) 1319
EcoRII CCWGG 1 cut(s) 1041
EcoT14I CCWWGG 1 cut(s) 1272
EcoT38I GRGCYC 3 cut(s) 45, 144, 823
ErhI CCWWGG 1 cut(s) 1272
FaeI CATG 2 cut(s) 205, 887
FaiI YATR 8 cut(s) 203, 207, 471, 543, 761, 885, 1136, 1215
FalI AAGNNNNNCTT 2 cut(s) 594, 626
FaqI GGGAC 1 cut(s) 131
FatI CATG 2 cut(s) 201, 883
FauI CCCGC 1 cut(s) 845
Fnu4HI GCNGC 5 cut(s) 148, 176, 554, 966, 1160
FokI GGATG 7 cut(s) 314, 338, 744, 767, 842, 1000, 1221
FriOI GRGCYC 3 cut(s) 45, 144, 823
Fsp4HI GCNGC 5 cut(s) 148, 176, 554, 966, 1160
FspBI CTAG 4 cut(s) 414, 582, 588, 1333
GlaI GCGC 6 cut(s) 80, 107, 174, 184, 1163, 1175
GluI GCNGC 5 cut(s) 148, 176, 554, 966, 1160
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 798
HaeII RGCGCY 3 cut(s) 109, 186, 1165
HapII CCGG 6 cut(s) 109, 163, 218, 226, 985, 1261
HgaI GACGC 2 cut(s) 283, 371
HhaI GCGC 6 cut(s) 81, 108, 175, 185, 1164, 1176
Hin1I GRCGYC 1 cut(s) 275
Hin1II CATG 2 cut(s) 205, 887
Hin6I GCGC 6 cut(s) 79, 106, 173, 183, 1162, 1174
HinP1I GCGC 6 cut(s) 79, 106, 173, 183, 1162, 1174
HindIII AAGCTT 1 cut(s) 900
HinfI GANTC 7 cut(s) 119, 214, 713, 773, 782, 935, 1064
HpaII CCGG 6 cut(s) 109, 163, 218, 226, 985, 1261
HphI GGTGA 4 cut(s) 296, 371, 544, 665
Hpy166II GTNNAC 2 cut(s) 334, 1078
Hpy188III TCNNGA 2 cut(s) 218, 777
Hpy8I GTNNAC 2 cut(s) 334, 1078
Hpy99I CGWCG 5 cut(s) 277, 311, 314, 317, 320
HpyAV CCTTC 3 cut(s) 350, 431, 600
HpyCH4III ACNGT 4 cut(s) 502, 637, 653, 1061
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 4 cut(s) 679, 836, 1238, 1255
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 607, 853, 974
HpyF3I CTNAG 8 cut(s) 51, 102, 143, 597, 669, 687, 918, 1296
HpySE526I ACGT 1 cut(s) 330
Hsp92I GRCGYC 1 cut(s) 275
Hsp92II CATG 2 cut(s) 205, 887
HspAI GCGC 6 cut(s) 79, 106, 173, 183, 1162, 1174
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 3 cut(s) 290, 512, 805
LmnI GCTCC 4 cut(s) 92, 229, 263, 818
Lsp1109I GCAGC 3 cut(s) 565, 952, 1171
LweI GCATC 1 cut(s) 823
MaeI CTAG 4 cut(s) 414, 582, 588, 1333
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 9 cut(s) 359, 631, 653, 811, 996, 1055, 1117, 1186, 1263
MalI GATC 3 cut(s) 292, 514, 807
MbiI CCGCTC 1 cut(s) 282
MboI GATC 3 cut(s) 290, 512, 805
MboII GAAGA 9 cut(s) 142, 477, 759, 944, 950, 1001, 1004, 1035, 1038
MfeI CAATTG 2 cut(s) 85, 939
MhlI GDGCHC 3 cut(s) 45, 144, 823
MluCI AATT 7 cut(s) 14, 85, 795, 924, 939, 1001, 1250
MlyI GAGTC 3 cut(s) 113, 776, 782
MmeI TCCRAC 3 cut(s) 53, 352, 364
MroI TCCGGA 1 cut(s) 217
MseI TTAA 2 cut(s) 462, 1202
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 6 cut(s) 109, 163, 218, 226, 985, 1261
MspR9I CCNGG 4 cut(s) 110, 227, 986, 1043
MunI CAATTG 2 cut(s) 85, 939
Mva1269I GAATGC 2 cut(s) 856, 977
MvaI CCWGG 1 cut(s) 1043
MvnI CGCG 2 cut(s) 173, 1176
MwoI GCNNNNNNNGC 4 cut(s) 87, 607, 853, 974
NciI CCSGG 3 cut(s) 110, 227, 986
NdeII GATC 3 cut(s) 290, 512, 805
NlaIII CATG 2 cut(s) 205, 887
NlaIV GGNNCC 5 cut(s) 27, 126, 231, 265, 1320
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 7 cut(s) 359, 631, 653, 811, 1117, 1186, 1263
NspI RCATGY 2 cut(s) 205, 887
PcsI WCGNNNNNNNCGW 3 cut(s) 303, 312, 315
PctI GAATGC 2 cut(s) 856, 977
PfeI GAWTC 4 cut(s) 214, 713, 935, 1064
PflMI CCANNNNNTGG 1 cut(s) 547
PinAI ACCGGT 2 cut(s) 162, 1260
PkrI GCNGC 5 cut(s) 149, 177, 555, 967, 1161
PleI GAGTC 3 cut(s) 113, 776, 781
PpsI GAGTC 3 cut(s) 113, 776, 781
PpuMI RGGWCCY 1 cut(s) 1319
PshAI GACNNNNGTC 1 cut(s) 123
PsiI TTATAA 1 cut(s) 1136
Psp124BI GAGCTC 2 cut(s) 144, 823
Psp5II RGGWCCY 1 cut(s) 1319
Psp6I CCWGG 1 cut(s) 1041
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 5 cut(s) 27, 126, 231, 265, 1320
PspPI GGNCC 3 cut(s) 125, 220, 1319
PspPPI RGGWCCY 1 cut(s) 1319
RsaI GTAC 1 cut(s) 1259
RsaNI GTAC 1 cut(s) 1258
Rsr2I CGGWCCG 1 cut(s) 220
RsrII CGGWCCG 1 cut(s) 220
SacI GAGCTC 2 cut(s) 144, 823
SaqAI TTAA 2 cut(s) 462, 1202
SatI GCNGC 5 cut(s) 148, 176, 554, 966, 1160
Sau3AI GATC 3 cut(s) 290, 512, 805
Sau96I GGNCC 3 cut(s) 125, 220, 1319
SchI GAGTC 3 cut(s) 113, 776, 782
ScrFI CCNGG 4 cut(s) 110, 227, 986, 1043
SduI GDGCHC 3 cut(s) 45, 144, 823
SfaNI GCATC 1 cut(s) 823
SinI GGWCC 3 cut(s) 125, 220, 1319
Sse9I AATT 7 cut(s) 14, 85, 795, 924, 939, 1001, 1250
SsiI CCGC 9 cut(s) 90, 147, 176, 193, 223, 282, 852, 982, 1157
SspMI CTAG 4 cut(s) 414, 582, 588, 1333
SstI GAGCTC 2 cut(s) 144, 823
StyD4I CCNGG 4 cut(s) 108, 225, 984, 1041
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 4 cut(s) 502, 637, 653, 1061
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 257, 961
TaqII GACCGA 1 cut(s) 1140
TasI AATT 7 cut(s) 14, 85, 795, 924, 939, 1001, 1250
TauI GCSGC 2 cut(s) 150, 178
TfiI GAWTC 4 cut(s) 214, 713, 935, 1064
Tru1I TTAA 2 cut(s) 462, 1202
Tru9I TTAA 2 cut(s) 462, 1202
TscAI CASTG 1 cut(s) 1066
TseFI GTSAC 7 cut(s) 359, 631, 653, 811, 1117, 1186, 1263
TseI GCWGC 3 cut(s) 553, 965, 1159
Tsp45I GTSAC 7 cut(s) 359, 631, 653, 811, 1117, 1186, 1263
TspDTI ATGAA 5 cut(s) 142, 369, 578, 911, 1299
TspRI CASTG 1 cut(s) 1066
Van91I CCANNNNNTGG 1 cut(s) 547
VpaK11BI GGWCC 3 cut(s) 125, 220, 1319
XceI RCATGY 2 cut(s) 205, 887
XcmI CCANNNNNNNNNTGG 2 cut(s) 488, 1049
XspI CTAG 4 cut(s) 414, 582, 588, 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.