Rh1DG374200

U-box domain-containing protein 62-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006755
Physical Location & Seq
Reverse (-)
59776809 .. 59779644
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG374200.1

Sequence Viewer

Length: 1332 bp
ATGTCCTCCGAAGAACTGGGTCTCAACTCGCCGCAGCTAGTCTTCCACCAAGACGAGGCCTTGCGGTTCGGGGCACCGCCTCAGCGCCGGGTCGGGGACTCGGGTCCGACGAAGACCCGAGAGCTCAGCGGCTTCATCGACGATCGGTTCTTCCCGCCGCAAAACGCCGACTTCCGGCGGGGATTGTACAGCGAGAACCCGGAGCGGCGGGACCCGCCGGAGCCCCGGAACTGGAATCCGAATGCGACCGCCGTGAGGACGCCGAGCGGCGAGGGATCGGACGACGATGAGGATGAGGATGACGACGACGACGACGTCGAGGACGACGATGATGACGGCGACGTCGGAGGGTTGTTCGGAGTGGAGAATAGGAGCAAGGGCAATGGTGGTAATAATGTGAGTAATGGCGAGGACAAAATGGGAAATGGGAATGTTAAGCACCAGCAGCTCCATTCTTCTTTTGGTGGGGCTTTGATATTAGGATCAAGTAGGGAGGTGTTGGAGAAAGACCATAGTGTTAGCGTAGGGCAGCAGACACTGAATAACAACAACATCAGGGACAGTCCCAGTAATAATCATCATGAGCACCTGCCAGGAAGGGTTGGGAGTTATCAGAATGCAATCACGGTTGCTGAATCATCAGACGGCGACATGTATTACTCGCAGTATTTGCAGGGCTCGGAGGGCTCTGGCGCTGCTCAGAAGGATTTGGTTGTGGAAAATGGTTGTGGGTTTAGTGGAAGAAAGGATGCTTTGTGTTCAAGTGAGTCTGGAGACTCTTTGAGGGCGATTCTCTCTGATCCTGTGACGGGAGCTCTGATGGATGATGCAATGATATTGCCTTGTGGGCATTCATTTGGAGGCGGTGGAGTACAGCAAGTTATTAGAATGTTCTATTTGTTCCACGCTGAATTCCTCTATGCCAAAACTGGCATATCAGCATCCTTTTTACAGCCAGAACACTTTGGTCTGGCATTCCGTCGTGAAGAAGAGTTACACTTTCATCGCTCATCCAAAAGGAAAAGAGAAAGGTTTGACCAGGACAAGGGTGGTTATGGTGATTTAGCACTCACAGATCCTCCAAGAGGTAGAGGTGTTCAGTTTCCATTTGCTGTTATGGATCGAGTTATTATAAAGGGTAATAAGAGGACACCACAACGCTTCGTTGGGCGTGAGGCAGTTGTGACAACACAATGCTTAAATGGATGGTATGTGGTAAAGACATTGGATAATGCAGAGAGTGTAAAGTTGCAGTATCGCTCACTTGCCAAGGTACCAGATGATTCATCATCCAAGGCCATGCCGAGCAAGATGGCACCTAACTGGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

443

Amino Acids

48.69

Weight (kDa)

5.06

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB62-63_C PF23112 366 - 424 5e-35 U-box protein 62/63 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1133
AarI CACCTGC 1 cut(s) 597
AasI GACNNNNNNGTC 2 cut(s) 314, 341
AatII GACGTC 2 cut(s) 318, 345
Acc36I ACCTGC 1 cut(s) 597
Acc65I GGTACC 1 cut(s) 1273
AccB1I GGYRCC 3 cut(s) 73, 1273, 1315
AccBSI CCGCTC 2 cut(s) 205, 267
AclWI GGATC 5 cut(s) 283, 490, 794, 1070, 1128
AcsI RAATTY 1 cut(s) 911
AcyI GRCGYC 3 cut(s) 260, 315, 342
AfaI GTAC 3 cut(s) 188, 873, 1275
AfiI CCNNNNNNNGG 8 cut(s) 55, 94, 174, 231, 255, 809, 1045, 1085
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 1 cut(s) 762
AjnI CCWGG 2 cut(s) 592, 1038
AluBI AGCT 4 cut(s) 37, 124, 448, 815
AluI AGCT 4 cut(s) 37, 124, 448, 815
Alw21I GWGCWC 3 cut(s) 126, 588, 817
Alw26I GTCTC 2 cut(s) 26, 768
AlwI GGATC 5 cut(s) 283, 490, 794, 1070, 1128
AlwNI CAGNNNCTG 1 cut(s) 538
Ama87I CYCGRG 2 cut(s) 100, 117
AoxI GGCC 2 cut(s) 57, 1296
ApeKI GCWGC 4 cut(s) 34, 445, 529, 695
ApoI RAATTY 1 cut(s) 911
Asp718I GGTACC 1 cut(s) 1273
AspLEI GCGC 2 cut(s) 87, 695
AspS9I GGNCC 2 cut(s) 104, 211
AsuC2I CCSGG 3 cut(s) 89, 200, 226
AsuHPI GGTGA 1 cut(s) 1070
AvaI CYCGRG 2 cut(s) 100, 117
AvaII GGWCC 2 cut(s) 104, 211
BaeGI GKGCMC 1 cut(s) 76
BanI GGYRCC 3 cut(s) 73, 1273, 1315
BanII GRGCYC 5 cut(s) 126, 225, 680, 689, 817
BarI GAAGNNNNNNTAC 2 cut(s) 978, 1010
BbsI GAAGAC 2 cut(s) 34, 119
Bbv12I GWGCWC 3 cut(s) 126, 588, 817
BbvCI CCTCAGC 1 cut(s) 81
BbvI GCAGC 4 cut(s) 46, 457, 541, 682
BccI CCATC 3 cut(s) 814, 1200, 1306
BceAI ACGGC 3 cut(s) 236, 352, 661
BciT130I CCWGG 2 cut(s) 594, 1040
BcnI CCSGG 3 cut(s) 89, 200, 226
BcoDI GTCTC 2 cut(s) 26, 768
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 2 cut(s) 88, 696
BfuAI ACCTGC 1 cut(s) 597
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 9 cut(s) 32, 35, 130, 158, 206, 268, 446, 530, 696
BlpI GCTNAGC 1 cut(s) 125
BlsI GCNGC 9 cut(s) 33, 36, 131, 159, 207, 269, 447, 531, 697
Bme1390I CCNGG 5 cut(s) 89, 200, 226, 594, 1040
Bme18I GGWCC 2 cut(s) 104, 211
BmeT110I CYCGRG 2 cut(s) 100, 117
BmgT120I GGNCC 2 cut(s) 104, 211
BmiI GGNNCC 7 cut(s) 75, 105, 212, 213, 222, 1275, 1317
BmrFI CCNGG 5 cut(s) 89, 200, 226, 594, 1040
BmrI ACTGGG 2 cut(s) 26, 561
BmsI GCATC 3 cut(s) 739, 817, 950
BmuI ACTGGG 2 cut(s) 26, 561
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 2 cut(s) 34, 119
BpmI CTGGAG 1 cut(s) 792
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 125
BpuMI CCSGG 3 cut(s) 89, 200, 226
BsaHI GRCGYC 3 cut(s) 260, 315, 342
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 3 cut(s) 224, 1269, 1293
BsaXI ACNNNNNCTCC 2 cut(s) 1063, 1093
Bsc4I CCNNNNNNNGG 8 cut(s) 55, 94, 174, 231, 255, 809, 1045, 1085
Bse1I ACTGG 5 cut(s) 21, 236, 567, 934, 1328
Bse3DI GCAATG 2 cut(s) 388, 837
BseBI CCWGG 2 cut(s) 594, 1040
BseDI CCNNGG 3 cut(s) 224, 1269, 1293
BseGI GGATG 8 cut(s) 298, 304, 754, 829, 941, 1010, 1211, 1289
BseLI CCNNNNNNNGG 8 cut(s) 55, 94, 174, 231, 255, 809, 1045, 1085
BseMI GCAATG 2 cut(s) 388, 837
BseMII CTCAG 3 cut(s) 95, 139, 713
BseNI ACTGG 5 cut(s) 21, 236, 567, 934, 1328
BseSI GKGCMC 1 cut(s) 76
BseXI GCAGC 4 cut(s) 46, 457, 541, 682
Bsh1285I CGRYCG 2 cut(s) 145, 249
BshFI GGCC 2 cut(s) 59, 1298
BshNI GGYRCC 3 cut(s) 73, 1273, 1315
BsiEI CGRYCG 2 cut(s) 145, 249
BsiHKAI GWGCWC 3 cut(s) 126, 588, 817
BsiHKCI CYCGRG 2 cut(s) 100, 117
BsiSI CCGG 5 cut(s) 88, 175, 200, 218, 226
BslFI GGGAC 4 cut(s) 110, 224, 549, 572
BslI CCNNNNNNNGG 8 cut(s) 55, 94, 174, 231, 255, 809, 1045, 1085
BsmAI GTCTC 2 cut(s) 26, 768
BsmFI GGGAC 4 cut(s) 110, 224, 549, 572
BsmI GAATGC 4 cut(s) 247, 622, 850, 974
BsnI GGCC 2 cut(s) 59, 1298
Bso31I GGTCTC 1 cut(s) 26
BsoBI CYCGRG 2 cut(s) 100, 117
Bsp1286I GDGCHC 7 cut(s) 76, 126, 225, 588, 680, 689, 817
Bsp1407I TGTACA 1 cut(s) 186
Bsp143I GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
Bsp1720I GCTNAGC 1 cut(s) 125
BspANI GGCC 2 cut(s) 59, 1298
BspCNI CTCAG 3 cut(s) 94, 138, 712
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 7 cut(s) 75, 105, 212, 213, 222, 1275, 1317
BspMI ACCTGC 1 cut(s) 597
BspPI GGATC 5 cut(s) 283, 490, 794, 1070, 1128
BspT107I GGYRCC 3 cut(s) 73, 1273, 1315
BspTNI GGTCTC 1 cut(s) 26
BsrBI CCGCTC 2 cut(s) 205, 267
BsrDI GCAATG 2 cut(s) 388, 837
BsrGI TGTACA 1 cut(s) 186
BsrI ACTGG 5 cut(s) 21, 236, 567, 934, 1328
BssECI CCNNGG 3 cut(s) 224, 1269, 1293
BssMI GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
BssNI GRCGYC 3 cut(s) 260, 315, 342
BssT1I CCWWGG 2 cut(s) 1269, 1293
Bst2UI CCWGG 2 cut(s) 594, 1040
Bst4CI ACNGT 2 cut(s) 563, 628
Bst6I CTCTTC 1 cut(s) 984
BstACI GRCGYC 3 cut(s) 260, 315, 342
BstAPI GCANNNNNTGC 1 cut(s) 670
BstAUI TGTACA 1 cut(s) 186
BstDEI CTNAG 3 cut(s) 81, 125, 699
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 8 cut(s) 298, 304, 754, 829, 941, 1010, 1211, 1289
BstH2I RGCGCY 2 cut(s) 88, 696
BstHHI GCGC 2 cut(s) 87, 695
BstKTI GATC 6 cut(s) 145, 278, 485, 802, 1078, 1123
BstMAI GTCTC 2 cut(s) 26, 768
BstMBI GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
BstMCI CGRYCG 2 cut(s) 145, 249
BstMWI GCNNNNNNNGC 5 cut(s) 214, 445, 670, 684, 847
BstNI CCWGG 2 cut(s) 594, 1040
BstNSI RCATGY 1 cut(s) 655
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 5 cut(s) 87, 198, 224, 592, 1038
BstSLI GKGCMC 1 cut(s) 76
BstV1I GCAGC 4 cut(s) 46, 457, 541, 682
BstV2I GAAGAC 2 cut(s) 34, 119
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BsuRI GGCC 2 cut(s) 59, 1298
BtgZI GCGATG 1 cut(s) 989
BtsCI GGATG 8 cut(s) 298, 304, 754, 829, 941, 1010, 1211, 1289
BtsIMutI CAGTG 1 cut(s) 536
BveI ACCTGC 1 cut(s) 597
CaiI CAGNNNCTG 1 cut(s) 538
CciI TCATGA 1 cut(s) 580
CfoI GCGC 2 cut(s) 87, 695
Cfr13I GGNCC 2 cut(s) 104, 211
CseI GACGC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 187, 872, 1274
CviAII CATG 3 cut(s) 581, 652, 1300
CviQI GTAC 3 cut(s) 187, 872, 1274
DdeI CTNAG 3 cut(s) 81, 125, 699
DpnI GATC 6 cut(s) 144, 277, 484, 801, 1077, 1122
DpnII GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
DrdI GACNNNNNNGTC 2 cut(s) 314, 341
DseDI GACNNNNNNGTC 2 cut(s) 314, 341
Eam1104I CTCTTC 1 cut(s) 984
EarI CTCTTC 1 cut(s) 984
Ecl136II GAGCTC 2 cut(s) 124, 815
Eco130I CCWWGG 2 cut(s) 1269, 1293
Eco147I AGGCCT 1 cut(s) 59
Eco24I GRGCYC 5 cut(s) 126, 225, 680, 689, 817
Eco31I GGTCTC 1 cut(s) 26
Eco47I GGWCC 2 cut(s) 104, 211
Eco53kI GAGCTC 2 cut(s) 124, 815
Eco88I CYCGRG 2 cut(s) 100, 117
EcoICRI GAGCTC 2 cut(s) 124, 815
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoO109I RGGNCCY 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 911
EcoRII CCWGG 2 cut(s) 592, 1038
EcoT14I CCWWGG 2 cut(s) 1269, 1293
EcoT38I GRGCYC 5 cut(s) 126, 225, 680, 689, 817
ErhI CCWWGG 2 cut(s) 1269, 1293
FaeI CATG 3 cut(s) 584, 655, 1303
FaqI GGGAC 4 cut(s) 110, 224, 549, 572
FatI CATG 3 cut(s) 580, 651, 1299
FauI CCCGC 4 cut(s) 162, 171, 201, 222
Fnu4HI GCNGC 9 cut(s) 32, 35, 130, 158, 206, 268, 446, 530, 696
FokI GGATG 8 cut(s) 305, 311, 761, 836, 928, 997, 1218, 1276
FriOI GRGCYC 5 cut(s) 126, 225, 680, 689, 817
Fsp4HI GCNGC 9 cut(s) 32, 35, 130, 158, 206, 268, 446, 530, 696
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 86, 694
GluI GCNGC 9 cut(s) 32, 35, 130, 158, 206, 268, 446, 530, 696
GsuI CTGGAG 1 cut(s) 792
HaeII RGCGCY 2 cut(s) 88, 696
HaeIII GGCC 2 cut(s) 59, 1298
HapII CCGG 5 cut(s) 88, 175, 200, 218, 226
HgaI GACGC 1 cut(s) 268
HhaI GCGC 2 cut(s) 87, 695
Hin1I GRCGYC 3 cut(s) 260, 315, 342
Hin1II CATG 3 cut(s) 584, 655, 1303
Hin6I GCGC 2 cut(s) 85, 693
HinP1I GCGC 2 cut(s) 85, 693
HinfI GANTC 7 cut(s) 98, 235, 635, 767, 776, 790, 1283
HpaII CCGG 5 cut(s) 88, 175, 200, 218, 226
HphI GGTGA 1 cut(s) 1070
Hpy188III TCNNGA 3 cut(s) 581, 771, 983
HpyAV CCTTC 2 cut(s) 591, 697
HpyCH4III ACNGT 2 cut(s) 563, 628
HpyCH4IV ACGT 2 cut(s) 315, 342
HpyCH4V TGCA 5 cut(s) 620, 673, 830, 1235, 1252
HpyF10VI GCNNNNNNNGC 5 cut(s) 214, 445, 670, 684, 847
HpyF3I CTNAG 3 cut(s) 81, 125, 699
HpySE526I ACGT 2 cut(s) 315, 342
Hsp92I GRCGYC 3 cut(s) 260, 315, 342
Hsp92II CATG 3 cut(s) 584, 655, 1303
HspAI GCGC 2 cut(s) 85, 693
KflI GGGWCCC 1 cut(s) 211
KpnI GGTACC 1 cut(s) 1277
Kzo9I GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
LmnI GCTCC 5 cut(s) 202, 220, 372, 453, 812
Lsp1109I GCAGC 4 cut(s) 46, 457, 541, 682
LweI GCATC 3 cut(s) 739, 817, 950
MaeI CTAG 1 cut(s) 38
MaeII ACGT 2 cut(s) 315, 342
MaeIII GTNAC 3 cut(s) 805, 993, 1183
MalI GATC 6 cut(s) 144, 277, 484, 801, 1077, 1122
MbiI CCGCTC 2 cut(s) 205, 267
MboI GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
MboII GAAGA 8 cut(s) 23, 34, 124, 142, 447, 753, 998, 1001
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 7 cut(s) 76, 126, 225, 588, 680, 689, 817
MluCI AATT 1 cut(s) 911
MlyI GAGTC 3 cut(s) 92, 770, 776
MmeI TCCRAC 3 cut(s) 131, 325, 480
MseI TTAA 2 cut(s) 435, 1199
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 5 cut(s) 88, 175, 200, 218, 226
MspR9I CCNGG 5 cut(s) 89, 200, 226, 594, 1040
Mva1269I GAATGC 4 cut(s) 247, 622, 850, 974
MvaI CCWGG 2 cut(s) 594, 1040
MwoI GCNNNNNNNGC 5 cut(s) 214, 445, 670, 684, 847
NciI CCSGG 3 cut(s) 89, 200, 226
NdeII GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
NlaIII CATG 3 cut(s) 584, 655, 1303
NlaIV GGNNCC 7 cut(s) 75, 105, 212, 213, 222, 1275, 1317
NmeAIII GCCGAG 2 cut(s) 288, 1329
NmuCI GTSAC 2 cut(s) 805, 1183
NspI RCATGY 1 cut(s) 655
PagI TCATGA 1 cut(s) 580
PaqCI CACCTGC 1 cut(s) 597
PceI AGGCCT 1 cut(s) 59
PciI ACATGT 1 cut(s) 651
PcsI WCGNNNNNNNCGW 6 cut(s) 107, 309, 312, 315, 321, 324
PctI GAATGC 4 cut(s) 247, 622, 850, 974
PfeI GAWTC 4 cut(s) 235, 635, 790, 1283
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 9 cut(s) 33, 36, 131, 159, 207, 269, 447, 531, 697
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 3 cut(s) 92, 770, 775
PpsI GAGTC 3 cut(s) 92, 770, 775
PpuMI RGGWCCY 1 cut(s) 211
PscI ACATGT 1 cut(s) 651
PshAI GACNNNNGTC 1 cut(s) 102
PsiI TTATAA 1 cut(s) 1133
Psp124BI GAGCTC 2 cut(s) 126, 817
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 2 cut(s) 592, 1038
PspGI CCWGG 2 cut(s) 592, 1038
PspN4I GGNNCC 7 cut(s) 75, 105, 212, 213, 222, 1275, 1317
PspPI GGNCC 2 cut(s) 104, 211
PspPPI RGGWCCY 1 cut(s) 211
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 1075
PsyI GACNNNGTC 1 cut(s) 314
PvuI CGATCG 1 cut(s) 145
RsaI GTAC 3 cut(s) 188, 873, 1275
RsaNI GTAC 3 cut(s) 187, 872, 1274
SacI GAGCTC 2 cut(s) 126, 817
SaqAI TTAA 2 cut(s) 435, 1199
SatI GCNGC 9 cut(s) 32, 35, 130, 158, 206, 268, 446, 530, 696
Sau3AI GATC 6 cut(s) 142, 275, 482, 799, 1075, 1120
Sau96I GGNCC 2 cut(s) 104, 211
SchI GAGTC 3 cut(s) 92, 770, 776
ScrFI CCNGG 5 cut(s) 89, 200, 226, 594, 1040
SduI GDGCHC 7 cut(s) 76, 126, 225, 588, 680, 689, 817
SfaNI GCATC 3 cut(s) 739, 817, 950
SinI GGWCC 2 cut(s) 104, 211
Sse9I AATT 1 cut(s) 911
SseBI AGGCCT 1 cut(s) 59
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 2 cut(s) 126, 817
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 5 cut(s) 87, 198, 224, 592, 1038
StyI CCWWGG 2 cut(s) 1269, 1293
TaaI ACNGT 2 cut(s) 563, 628
TaiI ACGT 2 cut(s) 318, 345
TaqI TCGA 3 cut(s) 138, 318, 1123
TasI AATT 1 cut(s) 911
TatI WGTACW 2 cut(s) 186, 871
TauI GCSGC 5 cut(s) 34, 132, 160, 208, 270
TfiI GAWTC 4 cut(s) 235, 635, 790, 1283
Tru1I TTAA 2 cut(s) 435, 1199
Tru9I TTAA 2 cut(s) 435, 1199
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 2 cut(s) 805, 1183
TseI GCWGC 4 cut(s) 34, 445, 529, 695
Tsp45I GTSAC 2 cut(s) 805, 1183
TspDTI ATGAA 4 cut(s) 124, 843, 992, 1275
TspGWI ACGGA 1 cut(s) 968
TspRI CASTG 1 cut(s) 543
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 104, 211
XagI CCTNNNNNAGG 1 cut(s) 1083
XapI RAATTY 1 cut(s) 911
XceI RCATGY 1 cut(s) 655
XcmI CCANNNNNNNNNTGG 2 cut(s) 458, 1046
XspI CTAG 1 cut(s) 38
ZraI GACGTC 2 cut(s) 316, 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.