AT5G05270

Belongs to the chalcone isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014035
Physical Location & Seq
Forward (+)
1563286 .. 1565324
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05270.1

Sequence Viewer

Length: 630 bp
ATGGGAACAGAGATGGTCATGGTTCACGAGGTTCCTTTTCCTCCACAGATCATCACTTCCAAGCCACTCTCTCTTCTGGGCCAAGGGATCACAGACATTGAGATCCACTTTCTTCAAGTGAAGTTCACTGCGATCGGAGTTTACTTAGATCCTTCAGATGTTAAAACACATCTTGATAACTGGAAAGGCAAAACCGGAAAAGAACTCGCCGGCGATGATGACTTCTTCGACGCCCTTGCCTCCGCGGAGATGGAGAAGGTTATAAGAGTGGTGGTGATAAAGGAGATAAAAGGAGCTCAGTACGGAGTGCAGCTAGAGAATACGGTGAGAGATCGTTTGGCTGAGGAGGATAAGTACGAGGAAGAAGAAGAAACTGAGCTCGAGAAGGTCGTTGGCTTTTTCCAGTCCAAGTACTTCAAAGCTAACTCCGTTATCACTTACCATTTCTCAGCCAAAGATGGCATTTGCGAGATCGGGTTTGAGACGGAAGGTAAAGAAGAGGAGAAACTGAAGGTGGAGAATGCGAATGTGGTGGGAATGATGCAGAGATGGTATTTGTCCGGAAGTCGTGGAGTCTCACCGTCGACTATTGTCTCCATCGCTGATTCAATCTCCGCAGTTTTAACCTAA

Protein Analysis

209

Amino Acids

23.33

Weight (kDa)

4.74

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chalcone PF02431 10 - 206 1.2e-29 Chalcone-flavanone isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05270 AT5G05270
fragaria_vesca FvH4_7g25890 FvH4_7g25890
malus_domestica MD01G1167300.v1.1 MD07G1233400.v1.1
prunus_persica Prupe.2G263900_v2.0.a1
pyrus_communis pycom01g18040
rosa_laevigata RLG00000026916
rosa_multiflora Rmu_sc0001755.1_g000020
rosa_roxburghii Rroxscaffold_4G00285160
rosa_rugosa Rorug01G0370200
rosa_samantha Rh1AG378700 Rh1BG342000 Rh1CG355600 Rh1DG373100
rosa_wichuraiana Rw1G033320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 263
AccI GTMKAC 1 cut(s) 584
AccII CGCG 1 cut(s) 245
AccIII TCCGGA 1 cut(s) 560
AciI CCGC 3 cut(s) 243, 245, 615
AclWI GGATC 3 cut(s) 95, 97, 143
AcuI CTGAAG 2 cut(s) 138, 530
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 3 cut(s) 302, 356, 413
AgsI TTSAA 3 cut(s) 116, 418, 609
AluBI AGCT 4 cut(s) 296, 313, 379, 422
AluI AGCT 4 cut(s) 296, 313, 379, 422
Alw21I GWGCWC 2 cut(s) 298, 381
Alw26I GTCTC 3 cut(s) 476, 580, 598
AlwI GGATC 3 cut(s) 95, 97, 143
Ama87I CYCGRG 1 cut(s) 380
Aor13HI TCCGGA 1 cut(s) 560
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 310
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 3 cut(s) 286, 337, 570
AvaI CYCGRG 1 cut(s) 380
BanII GRGCYC 2 cut(s) 298, 381
BauI CACGAG 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 298, 381
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 322
BccI CCATC 5 cut(s) 7, 244, 452, 543, 605
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BcoDI GTCTC 3 cut(s) 476, 580, 598
BfaI CTAG 1 cut(s) 314
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 79
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 531
BoxI GACNNNNGTC 1 cut(s) 590
Bpu10I CCTNAGC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 82, 243
BsaWI WCCGGW 2 cut(s) 194, 560
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 2 cut(s) 185, 403
BseAI TCCGGA 1 cut(s) 560
BseDI CCNNGG 2 cut(s) 82, 243
BseMII CTCAG 4 cut(s) 311, 333, 366, 462
BseNI ACTGG 2 cut(s) 185, 403
BseRI GAGGAG 2 cut(s) 359, 515
BseXI GCAGC 1 cut(s) 322
BsgI GTGCAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 245
Bsh1285I CGRYCG 1 cut(s) 135
BshFI GGCC 1 cut(s) 81
BsiEI CGRYCG 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 298, 381
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 195, 210, 561
BsmAI GTCTC 3 cut(s) 476, 580, 598
BsmBI CGTCTC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 298, 381
Bsp13I TCCGGA 1 cut(s) 560
Bsp143I GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
BspACI CCGC 3 cut(s) 243, 245, 615
BspANI GGCC 1 cut(s) 81
BspCNI CTCAG 4 cut(s) 310, 334, 367, 461
BspEI TCCGGA 1 cut(s) 560
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 1 cut(s) 33
BspPI GGATC 3 cut(s) 95, 97, 143
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 2 cut(s) 185, 403
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 82, 243
BssMI GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 82
Bst2BI CACGAG 1 cut(s) 26
Bst4CI ACNGT 2 cut(s) 325, 582
Bst6I CTCTTC 2 cut(s) 78, 492
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 5 cut(s) 145, 297, 342, 375, 448
BstDSI CCRYGG 1 cut(s) 243
BstFNI CGCG 1 cut(s) 245
BstKTI GATC 7 cut(s) 51, 90, 105, 135, 151, 334, 474
BstMAI GTCTC 3 cut(s) 476, 580, 598
BstMBI GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
BstMCI CGRYCG 1 cut(s) 135
BstPAI GACNNNNGTC 1 cut(s) 590
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 322
BstX2I RGATCY 2 cut(s) 102, 148
BstYI RGATCY 2 cut(s) 102, 148
BsuRI GGCC 1 cut(s) 81
BtgI CCRYGG 1 cut(s) 243
BtgZI GCGATG 2 cut(s) 228, 583
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 79
Cfr42I CCGCGG 1 cut(s) 246
CseI GACGC 1 cut(s) 239
Csp6I GTAC 3 cut(s) 301, 355, 412
CviAII CATG 1 cut(s) 19
CviJI RGCY 9 cut(s) 64, 81, 296, 313, 341, 379, 396, 422, 452
CviKI_1 RGCY 9 cut(s) 64, 81, 296, 313, 341, 379, 396, 422, 452
CviQI GTAC 3 cut(s) 301, 355, 412
DdeI CTNAG 5 cut(s) 145, 297, 342, 375, 448
DpnI GATC 7 cut(s) 50, 89, 104, 134, 150, 333, 473
DpnII GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
Eam1104I CTCTTC 2 cut(s) 78, 492
EarI CTCTTC 2 cut(s) 78, 492
Ecl136II GAGCTC 2 cut(s) 296, 379
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 2 cut(s) 298, 381
Eco53kI GAGCTC 2 cut(s) 296, 379
Eco57I CTGAAG 2 cut(s) 138, 530
Eco88I CYCGRG 1 cut(s) 380
EcoICRI GAGCTC 2 cut(s) 296, 379
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 2 cut(s) 298, 381
ErhI CCWWGG 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 476
FaeI CATG 1 cut(s) 22
FaiI YATR 2 cut(s) 20, 263
FatI CATG 1 cut(s) 18
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 1 cut(s) 311
FriOI GRGCYC 2 cut(s) 298, 381
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 1 cut(s) 314
GluI GCNGC 1 cut(s) 311
HaeIII GGCC 1 cut(s) 81
HapII CCGG 3 cut(s) 195, 210, 561
HgaI GACGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 1 cut(s) 22
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HinfI GANTC 2 cut(s) 573, 605
HpaII CCGG 3 cut(s) 195, 210, 561
HphI GGTGA 3 cut(s) 286, 337, 570
Hpy166II GTNNAC 4 cut(s) 25, 126, 142, 585
Hpy188I TCNGA 2 cut(s) 137, 157
Hpy188III TCNNGA 4 cut(s) 26, 173, 382, 561
Hpy8I GTNNAC 4 cut(s) 25, 126, 142, 585
Hpy99I CGWCG 2 cut(s) 233, 586
HpyAV CCTTC 5 cut(s) 162, 250, 379, 482, 505
HpyCH4III ACNGT 2 cut(s) 325, 582
HpyCH4V TGCA 2 cut(s) 310, 544
HpyF3I CTNAG 5 cut(s) 145, 297, 342, 375, 448
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 1 cut(s) 22
Kpn2I TCCGGA 1 cut(s) 560
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
KspI CCGCGG 1 cut(s) 246
Kzo9I GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
LmnI GCTCC 1 cut(s) 293
LpnPI CCDG 6 cut(s) 62, 166, 208, 223, 416, 574
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 531
MaeI CTAG 1 cut(s) 314
MalI GATC 7 cut(s) 50, 89, 104, 134, 150, 333, 473
MboI GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
MboII GAAGA 7 cut(s) 65, 104, 217, 374, 377, 380, 509
MflI RGATCY 2 cut(s) 102, 148
MhlI GDGCHC 2 cut(s) 298, 381
MlyI GAGTC 1 cut(s) 582
MnlI CCTC 7 cut(s) 22, 51, 250, 337, 340, 352, 493
MreI CGCCGGCG 1 cut(s) 209
MroI TCCGGA 1 cut(s) 560
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 2 cut(s) 162, 623
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 3 cut(s) 195, 210, 561
Mva1269I GAATGC 1 cut(s) 526
MvnI CGCG 1 cut(s) 245
NaeI GCCGGC 1 cut(s) 211
NdeII GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 33
PaeR7I CTCGAG 1 cut(s) 380
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PctI GAATGC 1 cut(s) 526
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 605
PkrI GCNGC 1 cut(s) 312
Ple19I CGATCG 1 cut(s) 135
PleI GAGTC 1 cut(s) 581
PpsI GAGTC 1 cut(s) 581
PshAI GACNNNNGTC 1 cut(s) 590
PsiI TTATAA 1 cut(s) 263
Psp124BI GAGCTC 2 cut(s) 298, 381
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 79
PsuI RGATCY 2 cut(s) 102, 148
PvuI CGATCG 1 cut(s) 135
RsaI GTAC 3 cut(s) 302, 356, 413
RsaNI GTAC 3 cut(s) 301, 355, 412
SacI GAGCTC 2 cut(s) 298, 381
SacII CCGCGG 1 cut(s) 246
SalI GTCGAC 1 cut(s) 583
SaqAI TTAA 2 cut(s) 162, 623
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 7 cut(s) 48, 87, 102, 132, 148, 331, 471
Sau96I GGNCC 1 cut(s) 79
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 1 cut(s) 582
SduI GDGCHC 2 cut(s) 298, 381
SfaNI GCATC 1 cut(s) 531
Sfr274I CTCGAG 1 cut(s) 380
Sfr303I CCGCGG 1 cut(s) 246
SgrAI CRCCGGYG 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 246
SlaI CTCGAG 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SsiI CCGC 3 cut(s) 243, 245, 615
SspMI CTAG 1 cut(s) 314
SstI GAGCTC 2 cut(s) 298, 381
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 2 cut(s) 325, 582
TaqI TCGA 3 cut(s) 228, 381, 584
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 1 cut(s) 605
Tru1I TTAA 2 cut(s) 162, 623
Tru9I TTAA 2 cut(s) 162, 623
TscAI CASTG 1 cut(s) 133
TseI GCWGC 1 cut(s) 310
TspGWI ACGGA 3 cut(s) 318, 418, 500
TspRI CASTG 1 cut(s) 133
XhoI CTCGAG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 584
XspI CTAG 1 cut(s) 314
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.