AT5G06060

tropinone reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011641
Physical Location & Seq
Reverse (-)
1823839 .. 1825926
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06060.1

Sequence Viewer

Length: 795 bp
ATGGAAACTGACAAAAGATGGTCTCTCGCCGGGAAAACAGCTCTGGTAACCGGCGGGACTCGTGGAATCGGGAGAGCAGTTGTAGAGGAACTAGCAAAATTTGGTGCAAAAGTTCATACTTGTTCAAGGAACCAGGAAGAGCTAAATGCATGCTTGAATGATTGGAAAGCGAATGGTTTAGTCGTGTCTGGTTCGGTTTGTGATGCTTCGGTTAGGGATCAGAGGGAGAAGTTGATTCAGGAAGCTTCTTCTGCCTTCAGTGGCAAGCTCAACATCCTTATAAACAATGTTGGAACTAATGTCAGGAAACCAACAGTTGAATACTCAAGCGAGGAATATGCCAAAATCATGTCGACCAACTTAGAATCCGCTTTCCATTTATCTCAAATTGCTCATCCTCTTCTAAAAGCATCTGGTGTCGGAAGCATTGTGTTCATCTCCTCTGTAGCTGGCCTGGTGCATCTTAGCAGTGGATCTATCTATGGTGCAACTAAAGGAGCACTTAATCAGCTTACAAGAAATCTAGCTTGCGAGTGGGCAAGCGACAACATCAGAACCAATTGCGTGGCGCCATGGTACATCAAGACCTCACTTGTGGAAACGCTACTTGAGAAGAAAGAATTTGTGGAGGCTGTAGTTTCAAGGACCCCACTTGGGCGCGTTGGAGAACCAGAGGAAGTCTCATCGTTGGTTGCCTTTCTCTGCCTTCCCGCATCATCTTATATTACCGGACAGGTCATATCCGTCGATGGAGGATTCACTGTCAACGGTTTTAGCTATGCTATGAAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

264

Amino Acids

28.29

Weight (kDa)

7.6

Isoelectric Point (pI)

30.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SDR PF23441 9 - 253 1.3e-08 SDR-like rossmann domain
adh_short PF00106 12 - 205 1.1e-52 short chain dehydrogenase
KR PF08659 13 - 173 8.3e-14 KR domain
adh_short_C2 PF13561 21 - 254 1.8e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011641)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06060
fragaria_vesca FvH4_7g23050 FvH4_7g23050
malus_domestica MD01G1139100.v1.1
prunus_persica Prupe.2G239600_v2.0.a1 Prupe.2G239600_v2.0.a1 Prupe.2G239600_v2.0.a1
pyrus_communis pycom01g15970
rosa_chinensis RchiOBHm_Chr1g0368291
rosa_laevigata RLG00000027223
rosa_multiflora Rmu_sc0006033.1_g000017 Rmu_sc0009391.1_g000003
rosa_roxburghii Rroxscaffold_4G00288680
rosa_rugosa Rorug01G0339900
rosa_samantha Rh1AG347400 Rh1BG310400 Rh1CG325800 Rh1DG342200
rosa_wichuraiana Rw1G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 281
AccB1I GGYRCC 1 cut(s) 568
AccI GTMKAC 1 cut(s) 353
AccII CGCG 1 cut(s) 660
AciI CCGC 3 cut(s) 54, 369, 711
AclWI GGATC 2 cut(s) 225, 481
AcsI RAATTY 2 cut(s) 98, 620
AcuI CTGAAG 1 cut(s) 241
AcyI GRCGYC 1 cut(s) 569
AfaI GTAC 1 cut(s) 578
AfiI CCNNNNNNNGG 1 cut(s) 654
AgsI TTSAA 4 cut(s) 126, 157, 320, 642
AjnI CCWGG 2 cut(s) 132, 453
AluBI AGCT 8 cut(s) 41, 142, 245, 268, 449, 511, 527, 777
AluI AGCT 8 cut(s) 41, 142, 245, 268, 449, 511, 527, 777
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 27, 685
AlwI GGATC 2 cut(s) 225, 481
AoxI GGCC 1 cut(s) 451
ApoI RAATTY 2 cut(s) 98, 620
AspLEI GCGC 2 cut(s) 571, 660
AspS9I GGNCC 1 cut(s) 645
AsuC2I CCSGG 1 cut(s) 31
AvaII GGWCC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 568
BauI CACGAG 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 502
BccI CCATC 2 cut(s) 12, 743
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 2 cut(s) 134, 455
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 2 cut(s) 27, 685
BfaI CTAG 2 cut(s) 92, 524
BfmI CTRYAG 2 cut(s) 444, 633
BfoI RGCGCY 1 cut(s) 572
Bme1390I CCNGG 3 cut(s) 31, 134, 455
Bme18I GGWCC 1 cut(s) 645
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 3 cut(s) 131, 570, 647
BmrFI CCNGG 3 cut(s) 31, 134, 455
BmsI GCATC 4 cut(s) 193, 419, 469, 722
BplI GAGNNNNNCTC 2 cut(s) 665, 697
BpuEI CTTGAG 2 cut(s) 310, 629
BpuMI CCSGG 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 569
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 572
BsaWI WCCGGW 1 cut(s) 728
Bsc4I CCNNNNNNNGG 1 cut(s) 654
Bse118I RCCGGY 1 cut(s) 50
BseBI CCWGG 2 cut(s) 134, 455
BseDI CCNNGG 1 cut(s) 572
BseGI GGATG 2 cut(s) 273, 394
BseLI CCNNNNNNNGG 1 cut(s) 654
BseRI GAGGAG 1 cut(s) 430
Bsh1236I CGCG 1 cut(s) 660
BshFI GGCC 1 cut(s) 453
BshNI GGYRCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 502
BsiSI CCGG 3 cut(s) 30, 51, 729
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 654
BsmAI GTCTC 2 cut(s) 27, 685
BsmFI GGGAC 1 cut(s) 70
BsnI GGCC 1 cut(s) 453
Bso31I GGTCTC 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 2 cut(s) 217, 473
Bsp19I CCATGG 1 cut(s) 572
BspACI CCGC 3 cut(s) 54, 369, 711
BspANI GGCC 1 cut(s) 453
BspFNI CGCG 1 cut(s) 660
BspLI GGNNCC 3 cut(s) 131, 570, 647
BspPI GGATC 2 cut(s) 225, 481
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 1 cut(s) 572
BssMI GATC 2 cut(s) 217, 473
BssNI GRCGYC 1 cut(s) 569
BssSI CACGAG 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 572
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 2 cut(s) 134, 455
Bst4CI ACNGT 3 cut(s) 316, 763, 770
Bst6I CTCTTC 2 cut(s) 132, 405
BstACI GRCGYC 1 cut(s) 569
BstC8I GCNNGC 5 cut(s) 151, 266, 451, 529, 541
BstDEI CTNAG 2 cut(s) 361, 464
BstDSI CCRYGG 1 cut(s) 572
BstEII GGTNACC 1 cut(s) 46
BstF5I GGATG 2 cut(s) 273, 394
BstFNI CGCG 1 cut(s) 660
BstH2I RGCGCY 1 cut(s) 572
BstHHI GCGC 2 cut(s) 571, 660
BstKTI GATC 2 cut(s) 220, 476
BstMAI GTCTC 2 cut(s) 27, 685
BstMBI GATC 2 cut(s) 217, 473
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 2 cut(s) 134, 455
BstNSI RCATGY 1 cut(s) 153
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 3 cut(s) 29, 132, 453
BstSFI CTRYAG 2 cut(s) 444, 633
BstUI CGCG 1 cut(s) 660
BstX2I RGATCY 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 1 cut(s) 473
BsuRI GGCC 1 cut(s) 453
BtgI CCRYGG 1 cut(s) 572
BtsCI GGATG 2 cut(s) 273, 394
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 3 cut(s) 265, 475, 759
Cac8I GCNNGC 5 cut(s) 151, 266, 451, 529, 541
CfoI GCGC 2 cut(s) 571, 660
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 645
Csp6I GTAC 1 cut(s) 577
CviAII CATG 3 cut(s) 150, 349, 573
CviQI GTAC 1 cut(s) 577
DdeI CTNAG 2 cut(s) 361, 464
DinI GGCGCC 1 cut(s) 570
DpnI GATC 2 cut(s) 219, 475
DpnII GATC 2 cut(s) 217, 473
Eam1104I CTCTTC 2 cut(s) 132, 405
EarI CTCTTC 2 cut(s) 132, 405
Eco130I CCWWGG 1 cut(s) 572
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 241
Eco91I GGTNACC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 645
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 132, 453
EcoT14I CCWWGG 1 cut(s) 572
EcoT22I ATGCAT 1 cut(s) 151
EgeI GGCGCC 1 cut(s) 570
EheI GGCGCC 1 cut(s) 570
ErhI CCWWGG 1 cut(s) 572
FaeI CATG 3 cut(s) 153, 352, 576
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 1 cut(s) 70
FatI CATG 3 cut(s) 149, 348, 572
FauI CCCGC 2 cut(s) 47, 718
FblI GTMKAC 1 cut(s) 353
FokI GGATG 2 cut(s) 260, 381
FspBI CTAG 2 cut(s) 92, 524
GlaI GCGC 2 cut(s) 570, 659
HaeII RGCGCY 1 cut(s) 572
HaeIII GGCC 1 cut(s) 453
HapII CCGG 3 cut(s) 30, 51, 729
HhaI GCGC 2 cut(s) 571, 660
Hin1I GRCGYC 1 cut(s) 569
Hin1II CATG 3 cut(s) 153, 352, 576
Hin6I GCGC 2 cut(s) 569, 658
HinP1I GCGC 2 cut(s) 569, 658
HincII GTYRAC 2 cut(s) 354, 766
HindII GTYRAC 2 cut(s) 354, 766
HindIII AAGCTT 1 cut(s) 243
HinfI GANTC 5 cut(s) 58, 66, 235, 365, 756
HpaII CCGG 3 cut(s) 30, 51, 729
Hpy166II GTNNAC 2 cut(s) 354, 766
Hpy188I TCNGA 3 cut(s) 222, 422, 554
Hpy188III TCNNGA 4 cut(s) 70, 239, 304, 583
Hpy8I GTNNAC 2 cut(s) 354, 766
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 265, 716
HpyCH4III ACNGT 3 cut(s) 316, 763, 770
HpyCH4V TGCA 4 cut(s) 107, 149, 460, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 361, 464
Hsp92I GRCGYC 1 cut(s) 569
Hsp92II CATG 3 cut(s) 153, 352, 576
HspAI GCGC 2 cut(s) 569, 658
KasI GGCGCC 1 cut(s) 568
Kzo9I GATC 2 cut(s) 217, 473
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 1 cut(s) 497
LweI GCATC 4 cut(s) 193, 419, 469, 722
MaeI CTAG 2 cut(s) 92, 524
MaeIII GTNAC 1 cut(s) 46
MalI GATC 2 cut(s) 219, 475
MboI GATC 2 cut(s) 217, 473
MboII GAAGA 4 cut(s) 149, 240, 392, 625
MfeI CAATTG 1 cut(s) 559
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 4 cut(s) 98, 387, 559, 620
Mly113I GGCGCC 1 cut(s) 569
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 3 cut(s) 271, 400, 643
MnlI CCTC 9 cut(s) 79, 216, 325, 408, 451, 598, 622, 667, 746
Mph1103I ATGCAT 1 cut(s) 151
MseI TTAA 2 cut(s) 504, 793
MspI CCGG 3 cut(s) 30, 51, 729
MspR9I CCNGG 3 cut(s) 31, 134, 455
MunI CAATTG 1 cut(s) 559
MvaI CCWGG 2 cut(s) 134, 455
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 1 cut(s) 251
NarI GGCGCC 1 cut(s) 569
NciI CCSGG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 572
NdeII GATC 2 cut(s) 217, 473
NlaIII CATG 3 cut(s) 153, 352, 576
NlaIV GGNNCC 3 cut(s) 131, 570, 647
NsiI ATGCAT 1 cut(s) 151
NspI RCATGY 1 cut(s) 153
PaeI GCATGC 1 cut(s) 153
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 4 cut(s) 66, 235, 365, 756
PleI GAGTC 1 cut(s) 52
PluTI GGCGCC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 645
PsiI TTATAA 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 645
Psp6I CCWGG 2 cut(s) 132, 453
PspEI GGTNACC 1 cut(s) 46
PspGI CCWGG 2 cut(s) 132, 453
PspN4I GGNNCC 3 cut(s) 131, 570, 647
PspPI GGNCC 1 cut(s) 645
PspPPI RGGWCCY 1 cut(s) 645
PsuI RGATCY 1 cut(s) 473
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
SalI GTCGAC 1 cut(s) 352
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 504, 793
Sau3AI GATC 2 cut(s) 217, 473
Sau96I GGNCC 1 cut(s) 645
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 3 cut(s) 31, 134, 455
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 4 cut(s) 193, 419, 469, 722
SfcI CTRYAG 2 cut(s) 444, 633
SfoI GGCGCC 1 cut(s) 570
SinI GGWCC 1 cut(s) 645
SmlI CTYRAG 2 cut(s) 325, 608
SmoI CTYRAG 2 cut(s) 325, 608
SphI GCATGC 1 cut(s) 153
Sse9I AATT 4 cut(s) 98, 387, 559, 620
SsiI CCGC 3 cut(s) 54, 369, 711
SspDI GGCGCC 1 cut(s) 568
SspMI CTAG 2 cut(s) 92, 524
StyD4I CCNGG 3 cut(s) 29, 132, 453
StyI CCWWGG 1 cut(s) 572
TaaI ACNGT 3 cut(s) 316, 763, 770
TaqI TCGA 2 cut(s) 353, 747
TasI AATT 4 cut(s) 98, 387, 559, 620
TfiI GAWTC 4 cut(s) 66, 235, 365, 756
Tru1I TTAA 2 cut(s) 504, 793
Tru9I TTAA 2 cut(s) 504, 793
TscAI CASTG 3 cut(s) 265, 475, 766
TspDTI ATGAA 2 cut(s) 104, 424
TspGWI ACGGA 1 cut(s) 733
TspRI CASTG 3 cut(s) 265, 475, 766
VpaK11BI GGWCC 1 cut(s) 645
XapI RAATTY 2 cut(s) 98, 620
XceI RCATGY 1 cut(s) 153
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 2 cut(s) 92, 524
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.