MD01G1139100.v1.1

Tropinone reductase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011641
Physical Location & Seq
Forward (+)
24878823 .. 24882032
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1139100.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGAAACTTTAGTTTGGTACGTCTCTTCTGTAGTATTGGAGGCGCTGCCTCTTTGGCAACTTGCGATAAGTTTACAGTTCACTGAAGTTTGCTTGCAAACCGCGAAAATGGCCAAGGCAGAGAGCAATTACCCAAACGCCAGATGGTCTCTCCACGGCGGGGCTGCCCTCGTCACCGGCGGAACTCGTGGAATTGGGTACGCTTTGGTGGAGGAGCTAGCTGGGTTCGGCGCGGCGGTGCACACCTGTTCTCGAAACGAAGCAGAACTCTCCAAGTGCTTAGAGCAATGGAAGGCTAAGGGCTTTTCAGTCACTGGGTCAATCTGCGATGTTTCTTCCAAAACCGAGAGAGAAAAGCTCATCGAGCAAGTCGCTTCCACTTTCAATGGCAAGCTCAACATACTTGTAAACAATGTTGGGACTAACATCAGGAAGCCAACAACTGAGTACACTTCTGAAGAATATTCATCACTCATGGCTACCAACTTAGAATCTACATACCATCTTTCCCAACTTGCACATCCTCTTTTGAAAGCATCTGGGCGAGGAAGCATTGTGTTTGTATCCTCTGTTGCCGGGCTGGCCAGTATAGCTTCCGGGTCCATTTATGGAGCAAGTAAAGCTGCAATCAATCAGCTTACGAAAAATCTGGCTTGTGAGTGGGCGAAAGACAATATCAGGAGCAATGCTGTCTGCCCTTGGTACACTAGAACCTCACTTGTGGAACATTTGCTGGATAACAAGGAGTTTTTGGAAGAGATAACGGCGCGAACTCCGCTTGAGCGCGTTGCAGAGCCGGAGGAGGTGTCATCTCTGGTGGCGTTCCTTTGCCTGCCCGCCGCATCGTACATCACCGGGCAGATTATTTCTGTTGATGGAGGAATGACTGTAAACAGTTTCAACCCTACTAGGCGGCCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00960 ko01100 ko01110 map00960 map01100 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

308

Amino Acids

33.28

Weight (kDa)

6.02

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KR PF08659 54 - 215 3.2e-13 KR domain
adh_short PF00106 55 - 246 2.7e-53 short chain dehydrogenase
adh_short_C2 PF13561 63 - 296 8.9e-62 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011641)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06060
fragaria_vesca FvH4_7g23050 FvH4_7g23050
malus_domestica MD01G1139100.v1.1
prunus_persica Prupe.2G239600_v2.0.a1 Prupe.2G239600_v2.0.a1 Prupe.2G239600_v2.0.a1
pyrus_communis pycom01g15970
rosa_chinensis RchiOBHm_Chr1g0368291
rosa_laevigata RLG00000027223
rosa_multiflora Rmu_sc0006033.1_g000017 Rmu_sc0009391.1_g000003
rosa_roxburghii Rroxscaffold_4G00288680
rosa_rugosa Rorug01G0339900
rosa_samantha Rh1AG347400 Rh1BG310400 Rh1CG325800 Rh1DG342200
rosa_wichuraiana Rw1G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 104, 233, 769, 786
AciI CCGC 9 cut(s) 102, 159, 180, 233, 236, 776, 837, 840, 913
AcoI YGGCCR 3 cut(s) 111, 582, 914
AcuI CTGAAG 2 cut(s) 105, 477
AfaI GTAC 5 cut(s) 20, 200, 449, 704, 848
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 3 cut(s) 385, 532, 901
AluBI AGCT 7 cut(s) 217, 221, 358, 394, 593, 623, 637
AluI AGCT 7 cut(s) 217, 221, 358, 394, 593, 623, 637
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 2 cut(s) 28, 153
Alw44I GTGCAC 1 cut(s) 239
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 3 cut(s) 111, 582, 914
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 3 cut(s) 46, 164, 623
AspLEI GCGC 4 cut(s) 46, 233, 769, 786
AspS9I GGNCC 1 cut(s) 600
AsuC2I CCSGG 3 cut(s) 577, 598, 856
AsuHPI GGTGA 2 cut(s) 166, 844
AsuNHI GCTAGC 1 cut(s) 217
AvaII GGWCC 1 cut(s) 600
BaeGI GKGCMC 1 cut(s) 243
BalI TGGCCA 2 cut(s) 113, 584
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 33, 151, 610
BccI CCATC 3 cut(s) 138, 510, 869
BceAI ACGGC 2 cut(s) 172, 780
BciVI GTATCC 1 cut(s) 574
BcnI CCSGG 3 cut(s) 577, 598, 856
BcoDI GTCTC 2 cut(s) 28, 153
BfaI CTAG 3 cut(s) 218, 708, 909
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 47
BfuI GTATCC 1 cut(s) 574
BglI GCCNNNNNGGC 2 cut(s) 55, 581
BisI GCNGC 6 cut(s) 47, 165, 234, 624, 840, 914
BlsI GCNGC 6 cut(s) 48, 166, 235, 625, 841, 915
Bme1390I CCNGG 3 cut(s) 577, 598, 856
Bme18I GGWCC 1 cut(s) 600
BmgT120I GGNCC 1 cut(s) 600
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 3 cut(s) 577, 598, 856
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 545, 851
BmtI GCTAGC 1 cut(s) 221
BmuI ACTGGG 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 342, 374
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 800
BpuMI CCSGG 3 cut(s) 577, 598, 856
BsaI GGTCTC 1 cut(s) 153
BsaJI CCNNGG 3 cut(s) 114, 154, 698
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 2 cut(s) 319, 585
Bse3DI GCAATG 2 cut(s) 293, 691
BseDI CCNNGG 3 cut(s) 114, 154, 698
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMI GCAATG 2 cut(s) 293, 691
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 2 cut(s) 319, 585
BseRI GAGGAG 2 cut(s) 227, 815
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 3 cut(s) 33, 151, 610
BseYI CCCAGC 1 cut(s) 221
Bsh1236I CGCG 4 cut(s) 104, 233, 769, 786
BshFI GGCC 3 cut(s) 113, 584, 916
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 5 cut(s) 177, 576, 597, 797, 855
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 2 cut(s) 28, 153
BsmBI CGTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 3 cut(s) 113, 584, 916
Bso31I GGTCTC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 243
BspACI CCGC 9 cut(s) 102, 159, 180, 233, 236, 776, 837, 840, 913
BspANI GGCC 3 cut(s) 113, 584, 916
BspCNI CTCAG 1 cut(s) 436
BspFNI CGCG 4 cut(s) 104, 233, 769, 786
BspLI GGNNCC 1 cut(s) 601
BspOI GCTAGC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 153
BsrDI GCAATG 2 cut(s) 293, 691
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 2 cut(s) 319, 585
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 114, 154, 698
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 114, 698
Bst2BI CACGAG 1 cut(s) 186
Bst4CI ACNGT 3 cut(s) 78, 889, 896
Bst6I CTCTTC 2 cut(s) 31, 750
BstC8I GCNNGC 6 cut(s) 95, 219, 392, 582, 833, 837
BstDEI CTNAG 4 cut(s) 280, 297, 444, 487
BstDSI CCRYGG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 4 cut(s) 104, 233, 769, 786
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 4 cut(s) 46, 233, 769, 786
BstMAI GTCTC 2 cut(s) 28, 153
BstMWI GCNNNNNNNGC 7 cut(s) 55, 110, 364, 581, 590, 620, 775
BstSCI CCNGG 3 cut(s) 575, 596, 854
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 4 cut(s) 104, 233, 769, 786
BstV1I GCAGC 3 cut(s) 33, 151, 610
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 3 cut(s) 113, 584, 916
BtgI CCRYGG 1 cut(s) 154
BtgZI GCGATG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 520
BtsIMutI CAGTG 2 cut(s) 81, 312
Cac8I GCNNGC 6 cut(s) 95, 219, 392, 582, 833, 837
CaiI CAGNNNCTG 1 cut(s) 314
CfoI GCGC 4 cut(s) 46, 233, 769, 786
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 600
Csp6I GTAC 5 cut(s) 19, 199, 448, 703, 847
CviAII CATG 1 cut(s) 475
CviQI GTAC 5 cut(s) 19, 199, 448, 703, 847
DdeI CTNAG 4 cut(s) 280, 297, 444, 487
EaeI YGGCCR 3 cut(s) 111, 582, 914
Eam1104I CTCTTC 2 cut(s) 31, 750
EarI CTCTTC 2 cut(s) 31, 750
EciI GGCGGA 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 114, 698
Eco31I GGTCTC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 600
Eco57I CTGAAG 2 cut(s) 105, 477
EcoT14I CCWWGG 2 cut(s) 114, 698
ErhI CCWWGG 2 cut(s) 114, 698
Esp3I CGTCTC 1 cut(s) 28
FaeI CATG 1 cut(s) 478
FaiI YATR 5 cut(s) 401, 476, 499, 590, 609
FaqI GGGAC 1 cut(s) 433
FatI CATG 1 cut(s) 474
FauI CCCGC 2 cut(s) 152, 844
Fnu4HI GCNGC 6 cut(s) 47, 165, 234, 624, 840, 914
FokI GGATG 1 cut(s) 507
Fsp4HI GCNGC 6 cut(s) 47, 165, 234, 624, 840, 914
FspBI CTAG 3 cut(s) 218, 708, 909
GlaI GCGC 4 cut(s) 45, 232, 768, 785
GluI GCNGC 6 cut(s) 47, 165, 234, 624, 840, 914
GsaI CCCAGC 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 3 cut(s) 113, 584, 916
HapII CCGG 5 cut(s) 177, 576, 597, 797, 855
HhaI GCGC 4 cut(s) 46, 233, 769, 786
Hin1II CATG 1 cut(s) 478
Hin6I GCGC 4 cut(s) 44, 231, 767, 784
HinP1I GCGC 4 cut(s) 44, 231, 767, 784
HinfI GANTC 1 cut(s) 491
HpaII CCGG 5 cut(s) 177, 576, 597, 797, 855
HphI GGTGA 2 cut(s) 166, 844
Hpy166II GTNNAC 7 cut(s) 74, 81, 241, 409, 450, 705, 892
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 3 cut(s) 252, 430, 679
Hpy8I GTNNAC 7 cut(s) 74, 81, 241, 409, 450, 705, 892
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 3 cut(s) 78, 889, 896
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 5 cut(s) 97, 241, 518, 626, 791
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 110, 364, 581, 590, 620, 775
HpyF3I CTNAG 4 cut(s) 280, 297, 444, 487
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 1 cut(s) 478
HspAI GCGC 4 cut(s) 44, 231, 767, 784
LmnI GCTCC 3 cut(s) 214, 611, 681
Lsp1109I GCAGC 3 cut(s) 33, 151, 610
LweI GCATC 2 cut(s) 545, 851
MaeI CTAG 3 cut(s) 218, 708, 909
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 172, 310
MboII GAAGA 4 cut(s) 18, 327, 470, 767
MhlI GDGCHC 1 cut(s) 243
MlsI TGGCCA 2 cut(s) 113, 584
MluCI AATT 2 cut(s) 127, 192
MluNI TGGCCA 2 cut(s) 113, 584
Mox20I TGGCCA 2 cut(s) 113, 584
MscI TGGCCA 2 cut(s) 113, 584
Msp20I TGGCCA 2 cut(s) 113, 584
MspI CCGG 5 cut(s) 177, 576, 597, 797, 855
MspR9I CCNGG 3 cut(s) 577, 598, 856
MvnI CGCG 4 cut(s) 104, 233, 769, 786
MwoI GCNNNNNNNGC 7 cut(s) 55, 110, 364, 581, 590, 620, 775
NciI CCSGG 3 cut(s) 577, 598, 856
NheI GCTAGC 1 cut(s) 217
NlaIII CATG 1 cut(s) 478
NlaIV GGNNCC 1 cut(s) 601
NmuCI GTSAC 2 cut(s) 172, 310
PfeI GAWTC 1 cut(s) 491
PkrI GCNGC 6 cut(s) 48, 166, 235, 625, 841, 915
PspFI CCCAGC 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 1 cut(s) 600
PstNI CAGNNNCTG 1 cut(s) 314
RsaI GTAC 5 cut(s) 20, 200, 449, 704, 848
RsaNI GTAC 5 cut(s) 19, 199, 448, 703, 847
SatI GCNGC 6 cut(s) 47, 165, 234, 624, 840, 914
Sau96I GGNCC 1 cut(s) 600
ScrFI CCNGG 3 cut(s) 577, 598, 856
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 2 cut(s) 545, 851
SfcI CTRYAG 1 cut(s) 30
SgrAI CRCCGGYG 1 cut(s) 176
SinI GGWCC 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 2 cut(s) 127, 192
SsiI CCGC 9 cut(s) 102, 159, 180, 233, 236, 776, 837, 840, 913
SspI AATATT 1 cut(s) 464
SspMI CTAG 3 cut(s) 218, 708, 909
StyD4I CCNGG 3 cut(s) 575, 596, 854
StyI CCWWGG 2 cut(s) 114, 698
TaaI ACNGT 3 cut(s) 78, 889, 896
TaiI ACGT 1 cut(s) 24
TaqI TCGA 2 cut(s) 253, 363
TasI AATT 2 cut(s) 127, 192
TatI WGTACW 1 cut(s) 447
TauI GCSGC 3 cut(s) 236, 842, 916
TfiI GAWTC 1 cut(s) 491
TscAI CASTG 2 cut(s) 88, 319
TseFI GTSAC 2 cut(s) 172, 310
TseI GCWGC 3 cut(s) 46, 164, 623
Tsp45I GTSAC 2 cut(s) 172, 310
TspDTI ATGAA 1 cut(s) 456
TspRI CASTG 2 cut(s) 88, 319
VneI GTGCAC 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 600
XcmI CCANNNNNNNNNTGG 1 cut(s) 141
XspI CTAG 3 cut(s) 218, 708, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.