AT5G07270

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011369
Physical Location & Seq
Forward (+)
2280560 .. 2283667
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G07270.1

Sequence Viewer

Length: 1542 bp
ATGGGTAATTCATTTGGGTGTTCTGCCTCCGGCGAGAGATTAGTGTCGGCGGCGAGAGATGGGGATTTCGTGGAAGCCAAGATGCTTCTCGATTGCAATCCATGTCTCGCCAAGTATTCAACTTTCGGTGGTCTTAATTCTCCTTTGCATTTCGCTGCAGCTAAAGGCCATAACGAGATCGTCGGATTGTTGCTCGAGAACGGAGCTGATGTGAATTCCAGAAATTATTGCGGCCAGACAGCATTGATGCAAGCTTGTAGATACGGACATTGGGAAGTTGTGCAAACTCTTCTGCTTTTCCGATGCAATGTTACAAGAGCTGATTATTTAGCTGGAAGAACAGCGCTTCATTTCGCTGCTGTGAATGGTCACGCCAGGTGTATTAGACTTGTCTTGGCTGATTTTTTACCTAGTGATAAACTTAACTCTCTTCCTGAAACTGGTGTTGTCACTGCAAAGAACAAATCTGAGCAAAGCGCATTGTCTAAATTTGTGAATAAGGCGGCTGATGGTGGAATAACAGCTCTTCACATGGCTGCCTTGAATGGATTATTTGATTGTGTGCAGCTTTTGCTTGATCTTGAGGCCAATGTTTCCGCTGTGACATTTCATTATGGAACATCAATGGATATGATTGGAGCTGGAAGCACGCCTTTACACTATGCTGCTTGTGGTGGGAATCTGAAATGTTGTCAGATTCTTCTTGCAAGAGGTGCAAGAAAGATGACATTGAACTGCAACGGGTGGCTACCTATTGACATAGCACGGATGTGGAGTCGTCATTGGTTAGAACCGTTGCTTTCACCAAATTCTGATGTTGTCATTCCAGCCTTTCCTCATTCCAACTACTTATCATTGCCCCTTTTGAGTATCCTCAACATTGCAAGAGAGTTCGGGTTGCAGTCTGCAACCATTGGAGATGAAGTCGATATCTGTGCCGTTTGCCTGGAAAGAACATGCACAGTTGCTGCTGAAGGTTGTGAGCATCAGCTATGTGTGAGATGTGCGTTATACCTTTGCTCTTCAAGCAATGTTCCCTCGGTGACAGTTGGCCCGCCCGGATCAATTCCTTGCCCTCTTTGTCGACATGGAATTACAGCGTTTAAACGACTCCCAAGCTCCCTGACAAGAGAAATGAAGTTACCCATGTCACTCGGGTTCTGTGCACCATGTATGCTTCACACGGGAGACACAACAGATCAATCATCACCGACATGCCCACCAACAGAACAACGCAGCAGCAAGACCCGTGCAGCCTCGGTTTCATCTGATATATTCTGTCCGGTAACATGCAGCCCATTCCCTTCAGTAAACATTCCAATGTGCACCTGCAACGAAGGGACATGTCCAAACTTTGAGACACATGGAACAGAGAGACATAGCGAAGAACACGTCGAATCTTCTCCTTCTCGAACAACAACTGAACAGGAGAAGATAGAAGAAGGGCAAAGACTTGGGAAAACAACAACTTGTTCAAGTATGTTCTGGGGAAGAAGAAGCTGTAGCAGAGAGAACCAATGCAACTCCGAGATCAATGCTTGA

Protein Analysis

513

Amino Acids

55.35

Weight (kDa)

6.46

Isoelectric Point (pI)

47.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 16 - 106 9.8e-14 Ankyrin repeats (3 copies)
Ank PF00023 46 - 75 2.8e-08 Ankyrin repeat
Ank_2 PF12796 49 - 132 7.5e-18 Ankyrin repeats (3 copies)
Ank_4 PF13637 49 - 98 8.2e-13 Ankyrin repeats (many copies)
Ank_5 PF13857 64 - 105 1.9e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 112 - 199 2.5e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 164 - 241 4.6e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 214 - 267 3.5e-06 Ankyrin repeats (3 copies)
Ank PF00023 215 - 239 8.2e-06 Ankyrin repeat
RING_XB3-XBAT31 PF24921 311 - 366 7.5e-18 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1337
Acc36I ACCTGC 1 cut(s) 1337
AccI GTMKAC 1 cut(s) 1084
AciI CCGC 5 cut(s) 50, 231, 503, 597, 1055
AclWI GGATC 1 cut(s) 1069
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 3 cut(s) 214, 488, 808
AcuI CTGAAG 2 cut(s) 993, 1290
AfeI AGCGCT 1 cut(s) 345
AfiI CCNNNNNNNGG 1 cut(s) 440
AflIII ACRYGT 2 cut(s) 1343, 1390
AgsI TTSAA 5 cut(s) 120, 544, 733, 1026, 1476
AjiI CACGTC 1 cut(s) 1393
AjnI CCWGG 2 cut(s) 374, 945
AleI CACNNNNGTG 1 cut(s) 769
Alw21I GWGCWC 2 cut(s) 1168, 1328
Alw26I GTCTC 4 cut(s) 110, 1182, 1352, 1369
Alw44I GTGCAC 2 cut(s) 1164, 1324
AlwI GGATC 1 cut(s) 1069
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 2 cut(s) 194, 1154
Aor51HI AGCGCT 1 cut(s) 345
AoxI GGCC 4 cut(s) 166, 232, 585, 1051
ApaLI GTGCAC 2 cut(s) 1164, 1324
ApoI RAATTY 3 cut(s) 214, 488, 808
ArsI GACNNNNNNTTYG 2 cut(s) 1377, 1409
AspLEI GCGC 2 cut(s) 346, 479
AspS9I GGNCC 1 cut(s) 1052
AsuC2I CCSGG 1 cut(s) 1059
AsuHPI GGTGA 3 cut(s) 795, 1054, 1200
AvaI CYCGRG 2 cut(s) 194, 1154
BaeGI GKGCMC 2 cut(s) 1168, 1328
Bbv12I GWGCWC 2 cut(s) 1168, 1328
BccI CCATC 2 cut(s) 53, 503
BceAI ACGGC 1 cut(s) 923
BcgI CGANNNNNNTGC 3 cut(s) 917, 951, 1517
BciT130I CCWGG 2 cut(s) 376, 947
BciVI GTATCC 1 cut(s) 881
BcnI CCSGG 1 cut(s) 1059
BcoDI GTCTC 4 cut(s) 110, 1182, 1352, 1369
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 2 cut(s) 156, 1501
BfoI RGCGCY 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 1337
BfuI GTATCC 1 cut(s) 881
Bme1390I CCNGG 3 cut(s) 376, 947, 1059
BmeT110I CYCGRG 2 cut(s) 194, 1154
BmgBI CACGTC 1 cut(s) 1393
BmgT120I GGNCC 1 cut(s) 1052
BmrFI CCNGG 3 cut(s) 376, 947, 1059
BmsI GCATC 4 cut(s) 72, 237, 293, 994
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 1059
BsaBI GATNNNNATC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 1038, 1257
BsaWI WCCGGW 1 cut(s) 1282
BsaXI ACNNNNNCTCC 4 cut(s) 195, 225, 909, 939
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse1I ACTGG 1 cut(s) 445
Bse3DI GCAATG 4 cut(s) 313, 854, 879, 1036
Bse8I GATNNNNATC 1 cut(s) 96
BseBI CCWGG 2 cut(s) 376, 947
BseDI CCNNGG 2 cut(s) 1038, 1257
BseGI GGATG 1 cut(s) 774
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMI GCAATG 4 cut(s) 313, 854, 879, 1036
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 1 cut(s) 445
BseSI GKGCMC 2 cut(s) 1168, 1328
BsgI GTGCAG 2 cut(s) 584, 1272
BshFI GGCC 4 cut(s) 168, 234, 587, 1053
BsiHKAI GWGCWC 2 cut(s) 1168, 1328
BsiHKCI CYCGRG 2 cut(s) 194, 1154
BsiSI CCGG 3 cut(s) 30, 1059, 1283
BslFI GGGAC 1 cut(s) 1354
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 4 cut(s) 110, 1182, 1352, 1369
BsmFI GGGAC 1 cut(s) 1354
BsnI GGCC 4 cut(s) 168, 234, 587, 1053
BsoBI CYCGRG 2 cut(s) 194, 1154
Bsp1286I GDGCHC 2 cut(s) 1168, 1328
Bsp143I GATC 5 cut(s) 177, 577, 1061, 1198, 1530
BspACI CCGC 5 cut(s) 50, 231, 503, 597, 1055
BspANI GGCC 4 cut(s) 168, 234, 587, 1053
BspCNI CTCAG 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 1337
BspPI GGATC 1 cut(s) 1069
BspQI GCTCTTC 2 cut(s) 531, 1027
BsrDI GCAATG 4 cut(s) 313, 854, 879, 1036
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 1038, 1257
BssMI GATC 5 cut(s) 177, 577, 1061, 1198, 1530
Bst2UI CCWGG 2 cut(s) 376, 947
Bst4CI ACNGT 3 cut(s) 795, 964, 1048
Bst6I CTCTTC 4 cut(s) 294, 435, 531, 1027
BstAPI GCANNNNNTGC 2 cut(s) 571, 713
BstC8I GCNNGC 3 cut(s) 252, 650, 1055
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 774
BstH2I RGCGCY 1 cut(s) 347
BstHHI GCGC 2 cut(s) 346, 479
BstKTI GATC 5 cut(s) 180, 580, 1064, 1201, 1533
BstMAI GTCTC 4 cut(s) 110, 1182, 1352, 1369
BstMBI GATC 5 cut(s) 177, 577, 1061, 1198, 1530
BstMWI GCNNNNNNNGC 3 cut(s) 571, 713, 1026
BstNI CCWGG 2 cut(s) 376, 947
BstNSI RCATGY 4 cut(s) 960, 1218, 1293, 1347
BstSCI CCNGG 3 cut(s) 374, 945, 1057
BstSFI CTRYAG 2 cut(s) 156, 1501
BstSLI GKGCMC 2 cut(s) 1168, 1328
BsuI GTATCC 1 cut(s) 881
BsuRI GGCC 4 cut(s) 168, 234, 587, 1053
BtrI CACGTC 1 cut(s) 1393
BtsCI GGATG 1 cut(s) 774
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 1 cut(s) 450
BveI ACCTGC 1 cut(s) 1337
Cac8I GCNNGC 3 cut(s) 252, 650, 1055
CaiI CAGNNNCTG 1 cut(s) 968
CfoI GCGC 2 cut(s) 346, 479
Cfr13I GGNCC 1 cut(s) 1052
DdeI CTNAG 1 cut(s) 468
DpnI GATC 5 cut(s) 179, 579, 1063, 1200, 1532
DpnII GATC 5 cut(s) 177, 577, 1061, 1198, 1530
DraI TTTAAA 1 cut(s) 1105
EaeI YGGCCR 1 cut(s) 232
Eam1104I CTCTTC 4 cut(s) 294, 435, 531, 1027
EarI CTCTTC 4 cut(s) 294, 435, 531, 1027
Eco32I GATATC 1 cut(s) 931
Eco47III AGCGCT 1 cut(s) 345
Eco57I CTGAAG 2 cut(s) 993, 1290
Eco88I CYCGRG 2 cut(s) 194, 1154
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 374, 945
EcoRV GATATC 1 cut(s) 931
FalI AAGNNNNNCTT 2 cut(s) 637, 669
FaqI GGGAC 1 cut(s) 1354
FauI CCCGC 1 cut(s) 1062
FblI GTMKAC 1 cut(s) 1084
FokI GGATG 1 cut(s) 781
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 345, 478
HaeII RGCGCY 1 cut(s) 347
HaeIII GGCC 4 cut(s) 168, 234, 587, 1053
HapII CCGG 3 cut(s) 30, 1059, 1283
HhaI GCGC 2 cut(s) 346, 479
Hin6I GCGC 2 cut(s) 344, 477
HinP1I GCGC 2 cut(s) 344, 477
HincII GTYRAC 1 cut(s) 1085
HindII GTYRAC 1 cut(s) 1085
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 5 cut(s) 679, 697, 775, 1110, 1397
HpaII CCGG 3 cut(s) 30, 1059, 1283
HphI GGTGA 3 cut(s) 795, 1054, 1200
Hpy166II GTNNAC 4 cut(s) 1085, 1166, 1312, 1326
Hpy188I TCNGA 8 cut(s) 185, 302, 469, 684, 696, 814, 1270, 1528
Hpy188III TCNNGA 6 cut(s) 89, 196, 219, 434, 581, 1410
Hpy8I GTNNAC 4 cut(s) 1085, 1166, 1312, 1326
Hpy99I CGWCG 2 cut(s) 185, 1397
HpyAV CCTTC 5 cut(s) 968, 1314, 1331, 1416, 1436
HpyCH4III ACNGT 3 cut(s) 795, 964, 1048
HpyCH4IV ACGT 1 cut(s) 1392
HpyF10VI GCNNNNNNNGC 3 cut(s) 571, 713, 1026
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 1 cut(s) 1392
HspAI GCGC 2 cut(s) 344, 477
Kzo9I GATC 5 cut(s) 177, 577, 1061, 1198, 1530
LguI GCTCTTC 2 cut(s) 531, 1027
LmnI GCTCC 3 cut(s) 203, 638, 1124
LweI GCATC 4 cut(s) 72, 237, 293, 994
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 1392
MaeIII GTNAC 8 cut(s) 310, 368, 448, 601, 1042, 1140, 1149, 1285
MalI GATC 5 cut(s) 179, 579, 1063, 1200, 1532
MboI GATC 5 cut(s) 177, 577, 1061, 1198, 1530
MhlI GDGCHC 2 cut(s) 1168, 1328
MluCI AATT 8 cut(s) 7, 136, 214, 223, 488, 808, 1065, 1092
MlyI GAGTC 2 cut(s) 784, 1104
MmeI TCCRAC 2 cut(s) 163, 867
MnlI CCTC 8 cut(s) 37, 577, 704, 846, 884, 1048, 1086, 1267
MseI TTAA 3 cut(s) 135, 423, 1104
MslI CAYNNNNRTG 4 cut(s) 16, 769, 1213, 1319
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 3 cut(s) 30, 1059, 1283
MspR9I CCNGG 3 cut(s) 376, 947, 1059
MssI GTTTAAAC 1 cut(s) 1105
MvaI CCWGG 2 cut(s) 376, 947
MwoI GCNNNNNNNGC 3 cut(s) 571, 713, 1026
NciI CCSGG 1 cut(s) 1059
NdeII GATC 5 cut(s) 177, 577, 1061, 1198, 1530
NmuCI GTSAC 5 cut(s) 368, 448, 601, 1042, 1149
NspI RCATGY 4 cut(s) 960, 1218, 1293, 1347
OliI CACNNNNGTG 1 cut(s) 769
PaeR7I CTCGAG 1 cut(s) 194
PaqCI CACCTGC 1 cut(s) 1337
PciI ACATGT 1 cut(s) 1343
PciSI GCTCTTC 2 cut(s) 531, 1027
PfeI GAWTC 3 cut(s) 679, 697, 1397
PleI GAGTC 2 cut(s) 783, 1104
PmeI GTTTAAAC 1 cut(s) 1105
PpsI GAGTC 2 cut(s) 783, 1104
PscI ACATGT 1 cut(s) 1343
Psp6I CCWGG 2 cut(s) 374, 945
PspGI CCWGG 2 cut(s) 374, 945
PspPI GGNCC 1 cut(s) 1052
PstI CTGCAG 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 968
RseI CAYNNNNRTG 4 cut(s) 16, 769, 1213, 1319
SalI GTCGAC 1 cut(s) 1083
SapI GCTCTTC 2 cut(s) 531, 1027
SaqAI TTAA 3 cut(s) 135, 423, 1104
Sau3AI GATC 5 cut(s) 177, 577, 1061, 1198, 1530
Sau96I GGNCC 1 cut(s) 1052
SchI GAGTC 2 cut(s) 784, 1104
ScrFI CCNGG 3 cut(s) 376, 947, 1059
SduI GDGCHC 2 cut(s) 1168, 1328
SfaNI GCATC 4 cut(s) 72, 237, 293, 994
SfcI CTRYAG 2 cut(s) 156, 1501
Sfr274I CTCGAG 1 cut(s) 194
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 4 cut(s) 16, 769, 1213, 1319
SmlI CTYRAG 2 cut(s) 194, 581
SmoI CTYRAG 2 cut(s) 194, 581
Sse9I AATT 8 cut(s) 7, 136, 214, 223, 488, 808, 1065, 1092
SsiI CCGC 5 cut(s) 50, 231, 503, 597, 1055
SspMI CTAG 1 cut(s) 411
StyD4I CCNGG 3 cut(s) 374, 945, 1057
TaaI ACNGT 3 cut(s) 795, 964, 1048
TaiI ACGT 1 cut(s) 1395
TaqI TCGA 6 cut(s) 90, 195, 927, 1084, 1395, 1411
TasI AATT 8 cut(s) 7, 136, 214, 223, 488, 808, 1065, 1092
TauI GCSGC 3 cut(s) 53, 234, 506
TfiI GAWTC 3 cut(s) 679, 697, 1397
Tru1I TTAA 3 cut(s) 135, 423, 1104
Tru9I TTAA 3 cut(s) 135, 423, 1104
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 5 cut(s) 368, 448, 601, 1042, 1149
Tsp45I GTSAC 5 cut(s) 368, 448, 601, 1042, 1149
TspDTI ATGAA 5 cut(s) 338, 599, 936, 1151, 1254
TspGWI ACGGA 3 cut(s) 216, 279, 781
TspRI CASTG 1 cut(s) 457
VneI GTGCAC 2 cut(s) 1164, 1324
XapI RAATTY 3 cut(s) 214, 488, 808
XceI RCATGY 4 cut(s) 960, 1218, 1293, 1347
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 1084
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.