Rroxscaffold_4G00281630

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011369
Physical Location & Seq
Reverse (-)
3851455 .. 3856097
4643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281630.1

Sequence Viewer

Length: 1482 bp
ATGGGGAATTCATTTGGGTGCTCTGCCTCCGGCGAGAGGCTGGTATCGGCGGCGAGAGATGGAGATGTGATTGAAGCGAAGATGTTATTGGATTGCAACCCTTGCCTTGCTAAGTACTCAACCTTTGGTGGGCTTAACTCTCCTCTCCATTTCGCCGCCGCAAAAGGCCACAACGAGATTGTTTCGTTGCTTCTGGAGAATGGAGCTGATGTCAATTCCAGAAATTACTGTGGGCAGACAGCATTGATGCAAGCTTGTCGATATGGACATTGGGAAGTTTTGCAAACGCTCCTGCTGTTTAGGTGCAATGTTACAAGAGCAGACTACCTTACAGGCAGAACAGCTCTTCATTTTGCTGCTGTAAATGGACATGTAAGATGTATAAGACTTGTTGTGGCTGACTTTGTTCCAAGTGTTCCTTATGAAGCTATGAATTCTCAGATAGATAGTGACAGAGGTGATGGTGCAAATGCGACAAGTAAAAATGAGCAAAGTGCTCTGTCCAAGTTTGTAAATAAGCCAGCAGATTGTGGCATTACTGCTCTTCATATGGCTGCATTGAATGGGTATTTTGATTGCGTACAGCTCCTACTTGATCTACATGCAAATGTGGCAGCTGTTACATTTCATTATGGAACGTCTGTGGATTTAATAGGTCTGGTTGCTATTAAGAGCCGGAAGTTCTCCATTGCACTACGCTGCTTGTGGGGGTGGCTTCCTCTTGATGTTGCCGGAATGTGGGGGCGCCACTGGCTTGAACCGCTGCTAGCACCCAATTCCGATGCAATAATACCGGCATTCCCCCTTTCTAATTACTTATCACTGCCTCTCATGAGCATACTGAACGTAGCAAGAGAGTGTGGATTCAAGTCTGGAACAACCTCAACCGATGAGGCTGATACTTGTGCTGTCTGCCTGGAAAGAGCATGTTTTGTTGCTGCAGAAGGATGTAAGCATGAGCTTTGTGTTAGATGTGCACTCTATCTCTGCTCAACAAGCAACATCCCTGCAGAAATGGTAGGCCCACCAGGGTCCATTCCATGCCCTTTTTGTAGGCATGGAATCATCTCGTTTGTAAAGTTGCCTGGTTCCCCAGCAAAGGAAAACAAAATTCCCATGTCACTCGGCCTCTGTACCCCATGCATGCTTCATCGTGACCCTCACCGTGAATCCCCAGCTGGTGCTCCAGAGATAAAAAAGAACCGTGTGGCTTCAGTTTCTTCAGAACTACTCTGCCCGGTCACTTGTAGTCCATTTCCTTCCGTTGCCATTCCATTGTGCACTTGCAATGATGGTCCATGCCCACCATTTGAATCCCGGAATGCAGAAACTCAAGATGAATTTCCCAGTTCACAAGCGACATCAACAGACCAGAGTAAAATGGAAGGGCCAAGACTTGAAAAAACAAGCTGTTCAAACATGTTCTGGAGCAGAAGAAGCTGCAGCAGAGAGCATCAGTGCAATGCTGAGATAAACGCTTGA

Protein Analysis

493

Amino Acids

53.28

Weight (kDa)

6.39

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 16 - 106 7.5e-14 Ankyrin repeats (3 copies)
Ank PF00023 46 - 75 8.2e-09 Ankyrin repeat
Ank_2 PF12796 49 - 132 7.8e-18 Ankyrin repeats (3 copies)
Ank_4 PF13637 49 - 98 3.6e-13 Ankyrin repeats (many copies)
Ank_5 PF13857 64 - 109 4.3e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 106 - 204 1.8e-09 Ankyrin repeats (3 copies)
Ank_5 PF13857 172 - 217 2.1e-06 Ankyrin repeats (many copies)
Ank PF00023 178 - 207 2.4e-06 Ankyrin repeat
RING_XB3-XBAT31 PF24921 301 - 356 1.4e-17 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 744
AciI CCGC 4 cut(s) 50, 156, 159, 761
AcsI RAATTY 4 cut(s) 7, 433, 1110, 1340
AcuI CTGAAG 2 cut(s) 1197, 1206
AcyI GRCGYC 1 cut(s) 745
AfaI GTAC 3 cut(s) 116, 582, 1135
AfiI CCNNNNNNNGG 4 cut(s) 36, 129, 738, 1099
AflIII ACRYGT 2 cut(s) 370, 1419
AgsI TTSAA 7 cut(s) 74, 562, 758, 868, 1313, 1400, 1416
AjnI CCWGG 3 cut(s) 915, 1027, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
Alw21I GWGCWC 5 cut(s) 23, 499, 979, 1186, 1283
Alw44I GTGCAC 2 cut(s) 975, 1279
AoxI GGCC 4 cut(s) 166, 1021, 1126, 1388
ApaLI GTGCAC 2 cut(s) 975, 1279
ApeKI GCWGC 8 cut(s) 356, 554, 614, 699, 763, 938, 1440, 1443
ApoI RAATTY 4 cut(s) 7, 433, 1110, 1340
AspLEI GCGC 1 cut(s) 747
AspS9I GGNCC 4 cut(s) 1022, 1032, 1295, 1388
AsuC2I CCSGG 2 cut(s) 1238, 1318
AsuHPI GGTGA 2 cut(s) 470, 1154
AsuNHI GCTAGC 1 cut(s) 766
AvaII GGWCC 2 cut(s) 1032, 1295
BaeGI GKGCMC 2 cut(s) 979, 1283
BanI GGYRCC 1 cut(s) 744
Bbv12I GWGCWC 5 cut(s) 23, 499, 979, 1186, 1283
BbvI GCAGC 8 cut(s) 343, 541, 626, 686, 750, 925, 1427, 1455
BccI CCATC 3 cut(s) 53, 455, 1286
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BciT130I CCWGG 3 cut(s) 917, 1029, 1086
BcnI CCSGG 2 cut(s) 1238, 1318
BfaI CTAG 1 cut(s) 767
BfmI CTRYAG 3 cut(s) 939, 1008, 1441
BfoI RGCGCY 1 cut(s) 748
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 5 cut(s) 917, 1029, 1086, 1238, 1318
Bme18I GGWCC 2 cut(s) 1032, 1295
BmgT120I GGNCC 4 cut(s) 1022, 1032, 1295, 1388
BmiI GGNNCC 3 cut(s) 746, 1033, 1090
BmrFI CCNGG 5 cut(s) 917, 1029, 1086, 1238, 1318
BmrI ACTGGG 1 cut(s) 1341
BmsI GCATC 3 cut(s) 237, 772, 1462
BmtI GCTAGC 1 cut(s) 770
BmuI ACTGGG 1 cut(s) 1341
BpmI CTGGAG 3 cut(s) 215, 1170, 1447
BpuEI CTTGAG 1 cut(s) 1317
BpuMI CCSGG 2 cut(s) 1238, 1318
BsaHI GRCGYC 1 cut(s) 745
BsaJI CCNNGG 1 cut(s) 1028
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 129, 738, 1099
Bse118I RCCGGY 1 cut(s) 793
Bse1I ACTGG 2 cut(s) 755, 1347
Bse3DI GCAATG 4 cut(s) 313, 687, 1294, 1468
BseBI CCWGG 3 cut(s) 917, 1029, 1086
BseDI CCNNGG 1 cut(s) 1028
BseGI GGATG 2 cut(s) 953, 1002
BseLI CCNNNNNNNGG 4 cut(s) 36, 129, 738, 1099
BseMI GCAATG 4 cut(s) 313, 687, 1294, 1468
BseMII CTCAG 2 cut(s) 452, 1458
BseNI ACTGG 2 cut(s) 755, 1347
BseRI GAGGAG 1 cut(s) 132
BseSI GKGCMC 2 cut(s) 979, 1283
BseXI GCAGC 8 cut(s) 343, 541, 626, 686, 750, 925, 1427, 1455
BseYI CCCAGC 2 cut(s) 1093, 1174
BshFI GGCC 4 cut(s) 168, 1023, 1128, 1390
BshNI GGYRCC 1 cut(s) 744
BsiHKAI GWGCWC 5 cut(s) 23, 499, 979, 1186, 1283
BsiSI CCGG 6 cut(s) 30, 676, 732, 794, 1238, 1318
BslI CCNNNNNNNGG 4 cut(s) 36, 129, 738, 1099
BsmI GAATGC 2 cut(s) 797, 1327
BsnI GGCC 4 cut(s) 168, 1023, 1128, 1390
Bsp1286I GDGCHC 5 cut(s) 23, 499, 979, 1186, 1283
Bsp143I GATC 1 cut(s) 595
BspACI CCGC 4 cut(s) 50, 156, 159, 761
BspANI GGCC 4 cut(s) 168, 1023, 1128, 1390
BspCNI CTCAG 2 cut(s) 451, 1459
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 3 cut(s) 746, 1033, 1090
BspMAI CTGCAG 3 cut(s) 943, 1012, 1445
BspOI GCTAGC 1 cut(s) 770
BspQI GCTCTTC 2 cut(s) 351, 549
BspT107I GGYRCC 1 cut(s) 744
BsrDI GCAATG 4 cut(s) 313, 687, 1294, 1468
BsrFI RCCGGY 1 cut(s) 793
BsrI ACTGG 2 cut(s) 755, 1347
BssAI RCCGGY 1 cut(s) 793
BssECI CCNNGG 1 cut(s) 1028
BssMI GATC 1 cut(s) 595
BssNI GRCGYC 1 cut(s) 745
Bst2UI CCWGG 3 cut(s) 917, 1029, 1086
Bst4CI ACNGT 3 cut(s) 230, 1166, 1205
Bst6I CTCTTC 2 cut(s) 351, 549
BstACI GRCGYC 1 cut(s) 745
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 4 cut(s) 252, 522, 768, 1145
BstDEI CTNAG 3 cut(s) 111, 438, 1467
BstF5I GGATG 2 cut(s) 953, 1002
BstH2I RGCGCY 1 cut(s) 748
BstHHI GCGC 1 cut(s) 747
BstKTI GATC 1 cut(s) 598
BstMBI GATC 1 cut(s) 595
BstMWI GCNNNNNNNGC 6 cut(s) 102, 611, 751, 760, 996, 1437
BstNI CCWGG 3 cut(s) 917, 1029, 1086
BstNSI RCATGY 5 cut(s) 374, 605, 930, 1147, 1423
BstSCI CCNGG 5 cut(s) 915, 1027, 1084, 1236, 1316
BstSFI CTRYAG 3 cut(s) 939, 1008, 1441
BstSLI GKGCMC 2 cut(s) 979, 1283
BstV1I GCAGC 8 cut(s) 343, 541, 626, 686, 750, 925, 1427, 1455
BsuRI GGCC 4 cut(s) 168, 1023, 1128, 1390
BtsCI GGATG 2 cut(s) 953, 1002
BtsI GCAGTG 1 cut(s) 821
BtsIMutI CAGTG 3 cut(s) 748, 821, 1463
Cac8I GCNNGC 4 cut(s) 252, 522, 768, 1145
CciI TCATGA 1 cut(s) 831
CfoI GCGC 1 cut(s) 747
Cfr10I RCCGGY 1 cut(s) 793
Cfr13I GGNCC 4 cut(s) 1022, 1032, 1295, 1388
Csp6I GTAC 3 cut(s) 115, 581, 1134
CviQI GTAC 3 cut(s) 115, 581, 1134
DdeI CTNAG 3 cut(s) 111, 438, 1467
DinI GGCGCC 1 cut(s) 746
DpnI GATC 1 cut(s) 597
DpnII GATC 1 cut(s) 595
Eam1104I CTCTTC 2 cut(s) 351, 549
EarI CTCTTC 2 cut(s) 351, 549
Eco47I GGWCC 2 cut(s) 1032, 1295
Eco57I CTGAAG 2 cut(s) 1197, 1206
EcoRI GAATTC 2 cut(s) 7, 433
EcoRII CCWGG 3 cut(s) 915, 1027, 1084
EcoT22I ATGCAT 1 cut(s) 1145
EgeI GGCGCC 1 cut(s) 746
EheI GGCGCC 1 cut(s) 746
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FauNDI CATATG 1 cut(s) 549
FokI GGATG 2 cut(s) 960, 989
FspBI CTAG 1 cut(s) 767
GlaI GCGC 1 cut(s) 746
GsaI CCCAGC 2 cut(s) 1097, 1178
GsuI CTGGAG 3 cut(s) 215, 1170, 1447
HaeII RGCGCY 1 cut(s) 748
HaeIII GGCC 4 cut(s) 168, 1023, 1128, 1390
HapII CCGG 6 cut(s) 30, 676, 732, 794, 1238, 1318
HhaI GCGC 1 cut(s) 747
Hin1I GRCGYC 1 cut(s) 745
Hin6I GCGC 1 cut(s) 745
HinP1I GCGC 1 cut(s) 745
HindIII AAGCTT 1 cut(s) 252
HinfI GANTC 4 cut(s) 864, 1062, 1169, 1313
HpaII CCGG 6 cut(s) 30, 676, 732, 794, 1238, 1318
HphI GGTGA 2 cut(s) 470, 1154
Hpy166II GTNNAC 3 cut(s) 977, 1281, 1352
Hpy188I TCNGA 3 cut(s) 441, 781, 1225
Hpy188III TCNNGA 9 cut(s) 194, 219, 722, 832, 873, 1154, 1187, 1334, 1426
Hpy8I GTNNAC 3 cut(s) 977, 1281, 1352
HpyAV CCTTC 3 cut(s) 938, 1269, 1379
HpyCH4III ACNGT 3 cut(s) 230, 1166, 1205
HpyCH4IV ACGT 2 cut(s) 638, 846
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 611, 751, 760, 996, 1437
HpyF3I CTNAG 3 cut(s) 111, 438, 1467
HpySE526I ACGT 2 cut(s) 638, 846
Hsp92I GRCGYC 1 cut(s) 745
HspAI GCGC 1 cut(s) 745
KasI GGCGCC 1 cut(s) 744
Kzo9I GATC 1 cut(s) 595
LguI GCTCTTC 2 cut(s) 351, 549
LmnI GCTCC 5 cut(s) 203, 294, 591, 1189, 1428
Lsp1109I GCAGC 8 cut(s) 343, 541, 626, 686, 750, 925, 1427, 1455
LweI GCATC 3 cut(s) 237, 772, 1462
MaeI CTAG 1 cut(s) 767
MaeII ACGT 2 cut(s) 638, 846
MaeIII GTNAC 6 cut(s) 310, 449, 619, 1119, 1154, 1240
MalI GATC 1 cut(s) 597
MboI GATC 1 cut(s) 595
MboII GAAGA 5 cut(s) 91, 338, 536, 1212, 1446
MhlI GDGCHC 5 cut(s) 23, 499, 979, 1186, 1283
MluCI AATT 8 cut(s) 7, 214, 223, 433, 775, 811, 1110, 1340
Mly113I GGCGCC 1 cut(s) 745
Mph1103I ATGCAT 1 cut(s) 1145
MseI TTAA 3 cut(s) 135, 650, 669
MslI CAYNNNNRTG 2 cut(s) 16, 606
MspA1I CMGCKG 3 cut(s) 617, 763, 1178
MspI CCGG 6 cut(s) 30, 676, 732, 794, 1238, 1318
MspR9I CCNGG 5 cut(s) 917, 1029, 1086, 1238, 1318
Mva1269I GAATGC 2 cut(s) 797, 1327
MvaI CCWGG 3 cut(s) 917, 1029, 1086
MwoI GCNNNNNNNGC 6 cut(s) 102, 611, 751, 760, 996, 1437
NarI GGCGCC 1 cut(s) 745
NciI CCSGG 2 cut(s) 1238, 1318
NdeI CATATG 1 cut(s) 549
NdeII GATC 1 cut(s) 595
NheI GCTAGC 1 cut(s) 766
NlaIV GGNNCC 3 cut(s) 746, 1033, 1090
NmeAIII GCCGAG 1 cut(s) 1104
NmuCI GTSAC 4 cut(s) 449, 1119, 1154, 1240
NsiI ATGCAT 1 cut(s) 1145
NspI RCATGY 5 cut(s) 374, 605, 930, 1147, 1423
PaeI GCATGC 1 cut(s) 1147
PagI TCATGA 1 cut(s) 831
PciI ACATGT 2 cut(s) 370, 1419
PciSI GCTCTTC 2 cut(s) 351, 549
PctI GAATGC 2 cut(s) 797, 1327
PfeI GAWTC 4 cut(s) 864, 1062, 1169, 1313
PfoI TCCNGGA 1 cut(s) 1316
PluTI GGCGCC 1 cut(s) 748
PscI ACATGT 2 cut(s) 370, 1419
Psp6I CCWGG 3 cut(s) 915, 1027, 1084
PspFI CCCAGC 2 cut(s) 1093, 1174
PspGI CCWGG 3 cut(s) 915, 1027, 1084
PspN4I GGNNCC 3 cut(s) 746, 1033, 1090
PspPI GGNCC 4 cut(s) 1022, 1032, 1295, 1388
PstI CTGCAG 3 cut(s) 943, 1012, 1445
PvuII CAGCTG 2 cut(s) 617, 1178
RsaI GTAC 3 cut(s) 116, 582, 1135
RsaNI GTAC 3 cut(s) 115, 581, 1134
RseI CAYNNNNRTG 2 cut(s) 16, 606
SapI GCTCTTC 2 cut(s) 351, 549
SaqAI TTAA 3 cut(s) 135, 650, 669
Sau3AI GATC 1 cut(s) 595
Sau96I GGNCC 4 cut(s) 1022, 1032, 1295, 1388
ScaI AGTACT 1 cut(s) 116
ScrFI CCNGG 5 cut(s) 917, 1029, 1086, 1238, 1318
SduI GDGCHC 5 cut(s) 23, 499, 979, 1186, 1283
SfaNI GCATC 3 cut(s) 237, 772, 1462
SfcI CTRYAG 3 cut(s) 939, 1008, 1441
SfoI GGCGCC 1 cut(s) 746
SinI GGWCC 2 cut(s) 1032, 1295
SmiMI CAYNNNNRTG 2 cut(s) 16, 606
SmlI CTYRAG 1 cut(s) 1332
SmoI CTYRAG 1 cut(s) 1332
SphI GCATGC 1 cut(s) 1147
Sse9I AATT 8 cut(s) 7, 214, 223, 433, 775, 811, 1110, 1340
SsiI CCGC 4 cut(s) 50, 156, 159, 761
SspDI GGCGCC 1 cut(s) 744
SspMI CTAG 1 cut(s) 767
StyD4I CCNGG 5 cut(s) 915, 1027, 1084, 1236, 1316
TaaI ACNGT 3 cut(s) 230, 1166, 1205
TaiI ACGT 2 cut(s) 641, 849
TaqI TCGA 1 cut(s) 259
TasI AATT 8 cut(s) 7, 214, 223, 433, 775, 811, 1110, 1340
TatI WGTACW 1 cut(s) 114
TauI GCSGC 3 cut(s) 53, 158, 161
TfiI GAWTC 4 cut(s) 864, 1062, 1169, 1313
Tru1I TTAA 3 cut(s) 135, 650, 669
Tru9I TTAA 3 cut(s) 135, 650, 669
TscAI CASTG 3 cut(s) 755, 828, 1463
TseFI GTSAC 4 cut(s) 449, 1119, 1154, 1240
TseI GCWGC 8 cut(s) 356, 554, 614, 699, 763, 938, 1440, 1443
Tsp45I GTSAC 4 cut(s) 449, 1119, 1154, 1240
TspDTI ATGAA 7 cut(s) 338, 438, 446, 536, 617, 1139, 1353
TspGWI ACGGA 1 cut(s) 1252
TspRI CASTG 3 cut(s) 755, 828, 1463
VneI GTGCAC 2 cut(s) 975, 1279
VpaK11BI GGWCC 2 cut(s) 1032, 1295
XapI RAATTY 4 cut(s) 7, 433, 1110, 1340
XceI RCATGY 5 cut(s) 374, 605, 930, 1147, 1423
XspI CTAG 1 cut(s) 767
ZrmI AGTACT 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 1145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.