RLG00000026559

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0011369
Physical Location & Seq
Reverse (-)
3829469 .. 3833271
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026559

Sequence Viewer

Length: 1461 bp
ATGGGGAATTCATTTGGGTGCTCTGCCTCCGGCGAGAGGCTGGTATCGGCGGCGAGAGATGGAGATGTGATTGAAGCGAAGATGTTATTGGATTGCAACCCTTGCCTTGCGAAAGTTTTGGAAAAGATACAGCTGTGGGATCTTTTGCATTGTGTGCAGCACTATTGTGCATTGATGCAAGCTTGTCGATATGGACATTGGGAAGTTTTGCAAACGCTTCTGCTGTTTAGGTGCAATGTTACAAGAGCAGACTACCTTACAGGCAGAACAGCTCTTCATTTTGCTGCTGTAAATGGACATGTAAGATGTATAAGACTTGTTGTGGCTGACTTTGTTCCAAGTGTTCTTTATGAAGCTATGAATTCTCAGATAGATAGTGACAGAGGTGACGGTGCAAATGCGACAAGTAAAAATGAGCAAAGTGCTCTGTCCAAGTTTGTAAATAAGCCAGCAGATTGTGGCATTACCGCTCTTCATATGGCTGCATTGAATGGGTATTTTGATTGCGTACAGCTCCTACTTGATCTTCATGCAAATGTGGCAGCTGTTACATTTCATTATGGAACGTCTGTGGATTTAATAGGAGCCGGAAGTTCTCCATTGCACTATGCTGCTTGTGGGGGTAACTTAAAATGCTGTCAGATCCTCCTTGCAAGAGGTGCTAGCAAGATGACTTTAAATTGCAATGGGTGGCTTCCTCTTGATGTTGCCAGAATGTGGGGGCGCCACTGGCTTGAACCGCTGCTAGCACCCAATTCCGATACAATAATACCGGCATTCCCCCTTTCTAATTACTTATCACTGCCTCTCATGAGCATACTGAACGTAGCAAGAGAGTGTGGATTCAAGTCTGGAACAACCTCAACCGATGAGGCTGATACTTGTGCTGTCTGCCTGGAAAGAGCATGTTTTGTTGCTGCAGAAGGATGTAAGCATGAGCTTTGTGTTAGATGCGCACTCTATCTCTGCTCAACAAGCAACATCCCTGCAGAAATGGTAGGCCCACCAGGGTCCATTCCATGCCCTTTTTGTAGGCATGGAATCATCTCGTTTGTGAAGTTGCCTGGTTCCCCAGCAAAGGAAAACAAAATACCCATGTCACTCGGCCTCTGTACCCCATGCATGCTTCATCGTGACCCACACCGTGAATCCCCAGCTTGTGCTCCAGAGATAAAAAAGAACCGTGTGGCCTCAGTTTCTTCAGAACTACTCTGCCCGGTCACTTGTAGTCCATTTCCTTCCGTTGCCATTCCATTGTGCACTTGCAATGATGGTCCATGCCCACCATTTGAATCCCGGAATGCAGAAACTCAAGATGAATTTCCCGGTTCACAAGCAACATCAACAGACCAGAGTAAAATGGAAGGGCCAAGACTTGAAAAAACAAGCTGTTCAAACATGTTCTGGAGCAGAAGAAGCTGCAGTAGAGAGCATCAGTGCAATGCTGAGATAAACGCTTGA

Protein Analysis

487

Amino Acids

52.68

Weight (kDa)

6.42

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 47 - 108 3.4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 58 - 180 1.2e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 58 - 106 7.9e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 148 - 193 2.1e-06 Ankyrin repeats (many copies)
Ank PF00023 154 - 183 2.3e-06 Ankyrin repeat
Ank_2 PF12796 196 - 244 1.3e-06 Ankyrin repeats (3 copies)
RING_XB3-XBAT31 PF24921 294 - 349 1.4e-17 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 955
AccB1I GGYRCC 1 cut(s) 723
AccB7I CCANNNNNTGG 1 cut(s) 717
AccBSI CCGCTC 1 cut(s) 470
AciI CCGC 3 cut(s) 50, 468, 740
AclWI GGATC 2 cut(s) 147, 637
AcsI RAATTY 3 cut(s) 7, 361, 1319
AcuI CTGAAG 1 cut(s) 1185
AcyI GRCGYC 1 cut(s) 724
AdeI CACNNNGTG 1 cut(s) 1145
AfaI GTAC 2 cut(s) 510, 1114
AfiI CCNNNNNNNGG 3 cut(s) 36, 717, 1078
AflIII ACRYGT 2 cut(s) 298, 1398
AgsI TTSAA 7 cut(s) 74, 490, 737, 847, 1292, 1379, 1395
AjnI CCWGG 3 cut(s) 894, 1006, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AleI CACNNNNGTG 1 cut(s) 165
Alw21I GWGCWC 4 cut(s) 23, 427, 1165, 1262
Alw44I GTGCAC 1 cut(s) 1258
AlwI GGATC 2 cut(s) 147, 637
AoxI GGCC 4 cut(s) 1000, 1105, 1188, 1367
ApaLI GTGCAC 1 cut(s) 1258
ApeKI GCWGC 8 cut(s) 157, 284, 482, 542, 611, 742, 917, 1419
ApoI RAATTY 3 cut(s) 7, 361, 1319
AspLEI GCGC 2 cut(s) 726, 956
AspS9I GGNCC 4 cut(s) 1001, 1011, 1274, 1367
AsuC2I CCSGG 3 cut(s) 1217, 1297, 1326
AsuHPI GGTGA 1 cut(s) 398
AsuNHI GCTAGC 2 cut(s) 662, 745
AvaII GGWCC 2 cut(s) 1011, 1274
BaeGI GKGCMC 1 cut(s) 1262
BaeI ACNNNNGTAYC 2 cut(s) 119, 152
BanI GGYRCC 1 cut(s) 723
Bbv12I GWGCWC 4 cut(s) 23, 427, 1165, 1262
BbvI GCAGC 8 cut(s) 169, 271, 469, 554, 598, 729, 904, 1406
BccI CCATC 2 cut(s) 53, 1265
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 3 cut(s) 896, 1008, 1065
BcnI CCSGG 3 cut(s) 1217, 1297, 1326
BfaI CTAG 2 cut(s) 663, 746
BfmI CTRYAG 3 cut(s) 918, 987, 1420
BfoI RGCGCY 1 cut(s) 727
BisI GCNGC 9 cut(s) 51, 158, 285, 483, 543, 612, 743, 918, 1420
BlsI GCNGC 9 cut(s) 52, 159, 286, 484, 544, 613, 744, 919, 1421
Bme1390I CCNGG 6 cut(s) 896, 1008, 1065, 1217, 1297, 1326
Bme18I GGWCC 2 cut(s) 1011, 1274
BmgT120I GGNCC 4 cut(s) 1001, 1011, 1274, 1367
BmiI GGNNCC 4 cut(s) 586, 725, 1012, 1069
BmrFI CCNGG 6 cut(s) 896, 1008, 1065, 1217, 1297, 1326
BmsI GCATC 3 cut(s) 165, 941, 1441
BmtI GCTAGC 2 cut(s) 666, 749
BpmI CTGGAG 2 cut(s) 1149, 1426
BpuEI CTTGAG 1 cut(s) 1296
BpuMI CCSGG 3 cut(s) 1217, 1297, 1326
BsaHI GRCGYC 1 cut(s) 724
BsaJI CCNNGG 1 cut(s) 1007
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 717, 1078
Bse118I RCCGGY 1 cut(s) 772
Bse1I ACTGG 1 cut(s) 734
Bse3DI GCAATG 5 cut(s) 241, 599, 691, 1273, 1447
BseBI CCWGG 3 cut(s) 896, 1008, 1065
BseDI CCNNGG 1 cut(s) 1007
BseGI GGATG 2 cut(s) 932, 981
BseLI CCNNNNNNNGG 3 cut(s) 36, 717, 1078
BseMI GCAATG 5 cut(s) 241, 599, 691, 1273, 1447
BseMII CTCAG 3 cut(s) 380, 1206, 1437
BseNI ACTGG 1 cut(s) 734
BseSI GKGCMC 1 cut(s) 1262
BseXI GCAGC 8 cut(s) 169, 271, 469, 554, 598, 729, 904, 1406
BseYI CCCAGC 2 cut(s) 1072, 1153
BsgI GTGCAG 1 cut(s) 176
BshFI GGCC 4 cut(s) 1002, 1107, 1190, 1369
BshNI GGYRCC 1 cut(s) 723
BsiHKAI GWGCWC 4 cut(s) 23, 427, 1165, 1262
BsiSI CCGG 6 cut(s) 30, 588, 773, 1217, 1297, 1326
BslI CCNNNNNNNGG 3 cut(s) 36, 717, 1078
BsmI GAATGC 2 cut(s) 776, 1306
BsnI GGCC 4 cut(s) 1002, 1107, 1190, 1369
Bsp1286I GDGCHC 4 cut(s) 23, 427, 1165, 1262
Bsp143I GATC 3 cut(s) 139, 523, 642
BspACI CCGC 3 cut(s) 50, 468, 740
BspANI GGCC 4 cut(s) 1002, 1107, 1190, 1369
BspCNI CTCAG 3 cut(s) 379, 1205, 1438
BspHI TCATGA 1 cut(s) 810
BspLI GGNNCC 4 cut(s) 586, 725, 1012, 1069
BspMAI CTGCAG 3 cut(s) 922, 991, 1424
BspOI GCTAGC 2 cut(s) 666, 749
BspPI GGATC 2 cut(s) 147, 637
BspQI GCTCTTC 2 cut(s) 279, 477
BspT107I GGYRCC 1 cut(s) 723
BsrBI CCGCTC 1 cut(s) 470
BsrDI GCAATG 5 cut(s) 241, 599, 691, 1273, 1447
BsrFI RCCGGY 1 cut(s) 772
BsrI ACTGG 1 cut(s) 734
BssAI RCCGGY 1 cut(s) 772
BssECI CCNNGG 1 cut(s) 1007
BssMI GATC 3 cut(s) 139, 523, 642
BssNI GRCGYC 1 cut(s) 724
Bst2UI CCWGG 3 cut(s) 896, 1008, 1065
Bst4CI ACNGT 3 cut(s) 392, 1145, 1184
Bst6I CTCTTC 2 cut(s) 279, 477
BstACI GRCGYC 1 cut(s) 724
BstAPI GCANNNNNTGC 3 cut(s) 102, 154, 659
BstC8I GCNNGC 5 cut(s) 180, 450, 664, 747, 1124
BstDEI CTNAG 3 cut(s) 366, 1192, 1446
BstF5I GGATG 2 cut(s) 932, 981
BstH2I RGCGCY 1 cut(s) 727
BstHHI GCGC 2 cut(s) 726, 956
BstKTI GATC 3 cut(s) 142, 526, 645
BstMBI GATC 3 cut(s) 139, 523, 642
BstMWI GCNNNNNNNGC 8 cut(s) 102, 154, 539, 659, 730, 739, 975, 1416
BstNI CCWGG 3 cut(s) 896, 1008, 1065
BstNSI RCATGY 4 cut(s) 302, 909, 1126, 1402
BstSCI CCNGG 6 cut(s) 894, 1006, 1063, 1215, 1295, 1324
BstSFI CTRYAG 3 cut(s) 918, 987, 1420
BstSLI GKGCMC 1 cut(s) 1262
BstV1I GCAGC 8 cut(s) 169, 271, 469, 554, 598, 729, 904, 1406
BstX2I RGATCY 2 cut(s) 139, 642
BstYI RGATCY 2 cut(s) 139, 642
BsuRI GGCC 4 cut(s) 1002, 1107, 1190, 1369
BtsCI GGATG 2 cut(s) 932, 981
BtsI GCAGTG 1 cut(s) 800
BtsIMutI CAGTG 3 cut(s) 727, 800, 1442
Cac8I GCNNGC 5 cut(s) 180, 450, 664, 747, 1124
CciI TCATGA 1 cut(s) 810
CfoI GCGC 2 cut(s) 726, 956
Cfr10I RCCGGY 1 cut(s) 772
Cfr13I GGNCC 4 cut(s) 1001, 1011, 1274, 1367
Csp6I GTAC 2 cut(s) 509, 1113
CviQI GTAC 2 cut(s) 509, 1113
DdeI CTNAG 3 cut(s) 366, 1192, 1446
DinI GGCGCC 1 cut(s) 725
DpnI GATC 3 cut(s) 141, 525, 644
DpnII GATC 3 cut(s) 139, 523, 642
DraI TTTAAA 1 cut(s) 678
DraIII CACNNNGTG 1 cut(s) 1145
Eam1104I CTCTTC 2 cut(s) 279, 477
EarI CTCTTC 2 cut(s) 279, 477
Eco47I GGWCC 2 cut(s) 1011, 1274
Eco57I CTGAAG 1 cut(s) 1185
EcoRI GAATTC 2 cut(s) 7, 361
EcoRII CCWGG 3 cut(s) 894, 1006, 1063
EcoT22I ATGCAT 1 cut(s) 1124
EgeI GGCGCC 1 cut(s) 725
EheI GGCGCC 1 cut(s) 725
FauNDI CATATG 1 cut(s) 477
Fnu4HI GCNGC 9 cut(s) 51, 158, 285, 483, 543, 612, 743, 918, 1420
FokI GGATG 2 cut(s) 939, 968
Fsp4HI GCNGC 9 cut(s) 51, 158, 285, 483, 543, 612, 743, 918, 1420
FspAI RTGCGCAY 1 cut(s) 955
FspBI CTAG 2 cut(s) 663, 746
FspI TGCGCA 1 cut(s) 955
GlaI GCGC 2 cut(s) 725, 955
GluI GCNGC 9 cut(s) 51, 158, 285, 483, 543, 612, 743, 918, 1420
GsaI CCCAGC 2 cut(s) 1076, 1157
GsuI CTGGAG 2 cut(s) 1149, 1426
HaeII RGCGCY 1 cut(s) 727
HaeIII GGCC 4 cut(s) 1002, 1107, 1190, 1369
HapII CCGG 6 cut(s) 30, 588, 773, 1217, 1297, 1326
HhaI GCGC 2 cut(s) 726, 956
Hin1I GRCGYC 1 cut(s) 724
Hin6I GCGC 2 cut(s) 724, 954
HinP1I GCGC 2 cut(s) 724, 954
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 4 cut(s) 843, 1041, 1148, 1292
HpaII CCGG 6 cut(s) 30, 588, 773, 1217, 1297, 1326
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 2 cut(s) 1260, 1331
Hpy188I TCNGA 4 cut(s) 369, 642, 760, 1204
Hpy188III TCNNGA 7 cut(s) 701, 811, 852, 1133, 1166, 1313, 1405
Hpy8I GTNNAC 2 cut(s) 1260, 1331
HpyAV CCTTC 3 cut(s) 917, 1248, 1358
HpyCH4III ACNGT 3 cut(s) 392, 1145, 1184
HpyCH4IV ACGT 2 cut(s) 566, 825
HpyF10VI GCNNNNNNNGC 8 cut(s) 102, 154, 539, 659, 730, 739, 975, 1416
HpyF3I CTNAG 3 cut(s) 366, 1192, 1446
HpySE526I ACGT 2 cut(s) 566, 825
Hsp92I GRCGYC 1 cut(s) 724
HspAI GCGC 2 cut(s) 724, 954
KasI GGCGCC 1 cut(s) 723
Kzo9I GATC 3 cut(s) 139, 523, 642
LguI GCTCTTC 2 cut(s) 279, 477
LmnI GCTCC 4 cut(s) 519, 584, 1168, 1407
Lsp1109I GCAGC 8 cut(s) 169, 271, 469, 554, 598, 729, 904, 1406
LweI GCATC 3 cut(s) 165, 941, 1441
MaeI CTAG 2 cut(s) 663, 746
MaeII ACGT 2 cut(s) 566, 825
MaeIII GTNAC 8 cut(s) 238, 377, 386, 547, 623, 1098, 1133, 1219
MalI GATC 3 cut(s) 141, 525, 644
MbiI CCGCTC 1 cut(s) 470
MboI GATC 3 cut(s) 139, 523, 642
MboII GAAGA 6 cut(s) 91, 266, 464, 518, 1191, 1425
MflI RGATCY 2 cut(s) 139, 642
MhlI GDGCHC 4 cut(s) 23, 427, 1165, 1262
MluCI AATT 6 cut(s) 7, 361, 679, 754, 790, 1319
Mly113I GGCGCC 1 cut(s) 724
Mph1103I ATGCAT 1 cut(s) 1124
MseI TTAA 3 cut(s) 578, 629, 677
MslI CAYNNNNRTG 3 cut(s) 16, 165, 534
MspA1I CMGCKG 3 cut(s) 133, 545, 742
MspI CCGG 6 cut(s) 30, 588, 773, 1217, 1297, 1326
MspR9I CCNGG 6 cut(s) 896, 1008, 1065, 1217, 1297, 1326
Mva1269I GAATGC 2 cut(s) 776, 1306
MvaI CCWGG 3 cut(s) 896, 1008, 1065
MwoI GCNNNNNNNGC 8 cut(s) 102, 154, 539, 659, 730, 739, 975, 1416
NarI GGCGCC 1 cut(s) 724
NciI CCSGG 3 cut(s) 1217, 1297, 1326
NdeI CATATG 1 cut(s) 477
NdeII GATC 3 cut(s) 139, 523, 642
NheI GCTAGC 2 cut(s) 662, 745
NlaIV GGNNCC 4 cut(s) 586, 725, 1012, 1069
NmeAIII GCCGAG 1 cut(s) 1083
NmuCI GTSAC 5 cut(s) 377, 386, 1098, 1133, 1219
NsbI TGCGCA 1 cut(s) 955
NsiI ATGCAT 1 cut(s) 1124
NspI RCATGY 4 cut(s) 302, 909, 1126, 1402
OliI CACNNNNGTG 1 cut(s) 165
PaeI GCATGC 1 cut(s) 1126
PagI TCATGA 1 cut(s) 810
PciI ACATGT 2 cut(s) 298, 1398
PciSI GCTCTTC 2 cut(s) 279, 477
PctI GAATGC 2 cut(s) 776, 1306
PfeI GAWTC 4 cut(s) 843, 1041, 1148, 1292
PflMI CCANNNNNTGG 1 cut(s) 717
PfoI TCCNGGA 1 cut(s) 1295
PkrI GCNGC 9 cut(s) 52, 159, 286, 484, 544, 613, 744, 919, 1421
PluTI GGCGCC 1 cut(s) 727
PscI ACATGT 2 cut(s) 298, 1398
Psp6I CCWGG 3 cut(s) 894, 1006, 1063
PspFI CCCAGC 2 cut(s) 1072, 1153
PspGI CCWGG 3 cut(s) 894, 1006, 1063
PspN4I GGNNCC 4 cut(s) 586, 725, 1012, 1069
PspPI GGNCC 4 cut(s) 1001, 1011, 1274, 1367
PstI CTGCAG 3 cut(s) 922, 991, 1424
PsuI RGATCY 2 cut(s) 139, 642
PvuII CAGCTG 2 cut(s) 133, 545
RsaI GTAC 2 cut(s) 510, 1114
RsaNI GTAC 2 cut(s) 509, 1113
RseI CAYNNNNRTG 3 cut(s) 16, 165, 534
SapI GCTCTTC 2 cut(s) 279, 477
SaqAI TTAA 3 cut(s) 578, 629, 677
SatI GCNGC 9 cut(s) 51, 158, 285, 483, 543, 612, 743, 918, 1420
Sau3AI GATC 3 cut(s) 139, 523, 642
Sau96I GGNCC 4 cut(s) 1001, 1011, 1274, 1367
ScrFI CCNGG 6 cut(s) 896, 1008, 1065, 1217, 1297, 1326
SduI GDGCHC 4 cut(s) 23, 427, 1165, 1262
SfaNI GCATC 3 cut(s) 165, 941, 1441
SfcI CTRYAG 3 cut(s) 918, 987, 1420
SfoI GGCGCC 1 cut(s) 725
SinI GGWCC 2 cut(s) 1011, 1274
SmiMI CAYNNNNRTG 3 cut(s) 16, 165, 534
SmlI CTYRAG 1 cut(s) 1311
SmoI CTYRAG 1 cut(s) 1311
SphI GCATGC 1 cut(s) 1126
Sse9I AATT 6 cut(s) 7, 361, 679, 754, 790, 1319
SsiI CCGC 3 cut(s) 50, 468, 740
SspDI GGCGCC 1 cut(s) 723
SspMI CTAG 2 cut(s) 663, 746
StyD4I CCNGG 6 cut(s) 894, 1006, 1063, 1215, 1295, 1324
TaaI ACNGT 3 cut(s) 392, 1145, 1184
TaiI ACGT 2 cut(s) 569, 828
TaqI TCGA 1 cut(s) 187
TasI AATT 6 cut(s) 7, 361, 679, 754, 790, 1319
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 4 cut(s) 843, 1041, 1148, 1292
Tru1I TTAA 3 cut(s) 578, 629, 677
Tru9I TTAA 3 cut(s) 578, 629, 677
TscAI CASTG 3 cut(s) 734, 807, 1442
TseFI GTSAC 5 cut(s) 377, 386, 1098, 1133, 1219
TseI GCWGC 8 cut(s) 157, 284, 482, 542, 611, 742, 917, 1419
Tsp45I GTSAC 5 cut(s) 377, 386, 1098, 1133, 1219
TspDTI ATGAA 8 cut(s) 266, 366, 374, 464, 518, 545, 1118, 1332
TspGWI ACGGA 1 cut(s) 1231
TspRI CASTG 3 cut(s) 734, 807, 1442
Van91I CCANNNNNTGG 1 cut(s) 717
VneI GTGCAC 1 cut(s) 1258
VpaK11BI GGWCC 2 cut(s) 1011, 1274
XapI RAATTY 3 cut(s) 7, 361, 1319
XceI RCATGY 4 cut(s) 302, 909, 1126, 1402
XspI CTAG 2 cut(s) 663, 746
Zsp2I ATGCAT 1 cut(s) 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.