AT5G12340

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012390
Physical Location & Seq
Reverse (-)
3992623 .. 3993767
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12340.1

Sequence Viewer

Length: 663 bp
ATGGGAAGATCTCTAATCAATTCTTGTTTTCCGATAATCCGTAGACGCCAAGGAATAGTCACGCTTGTTTTCTTTGAGCATGTATCAACAAAAACCCTATGTGGCTTCAAGAAACATGTTGCTGGAGAAATCATGTTTGAGTTTCCTGATCAAATCGTGTGCCATGCAGATTCTTTTTACATCGGTCGTCAGATTCCAGCTCTAGCCATGGACGACTATCTAATCCCAGGTCAAACTTATTTCGTCTTACCGATCGAACGTTTTCAATACAAAATCTTAACCACTTCGTGTCTCTCAATCTTCAACTCTAATCTTGAAAACGTTCGGTCAAACACTCCACCGTTGAATTTCACTGCGCCGTCGAGTCCACCACCGTTTGAGTATTCTCAGGGACCAAACGGAAAGATTCTGATAAAAGTTTCTCCGGAGTTTATCATTAGTCTTATATGTAAAAACAGAAACATCAGAGAAGAAGAAGCCACAAGTTGTGGTGAAAACAGTGCGATTTGTAATTCTCCCGAGTTAAAGAAGCAGTATGATCAGCTTGTTGGAACGAGAGAGCACATGTGGTCACCGAATTTGCAGACGATCTCCGAGCATAAGATTAGGGTTTCTCCATTAAGGTTCTTCGGTCTAAATCGGAAGCAAGATGAAGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

220

Amino Acids

25.11

Weight (kDa)

8.46

Isoelectric Point (pI)

59.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 199 1.1e-27 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 43
AccIII TCCGGA 1 cut(s) 424
AclI AACGTT 2 cut(s) 259, 321
AcsI RAATTY 2 cut(s) 346, 577
AcyI GRCGYC 1 cut(s) 46
AdeI CACNNNGTG 1 cut(s) 288
AflIII ACRYGT 2 cut(s) 115, 564
AgsI TTSAA 5 cut(s) 109, 266, 304, 317, 346
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 2 cut(s) 200, 544
AluI AGCT 2 cut(s) 200, 544
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 1 cut(s) 296
Ama87I CYCGRG 1 cut(s) 518
Aor13HI TCCGGA 1 cut(s) 424
ApoI RAATTY 2 cut(s) 346, 577
Asp700I GAANNNNTTC 2 cut(s) 261, 321
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 392
AsuHPI GGTGA 2 cut(s) 503, 564
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 564
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 2 cut(s) 148, 538
BcoDI GTCTC 1 cut(s) 296
BfaI CTAG 1 cut(s) 203
BglII AGATCT 1 cut(s) 8
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 1 cut(s) 392
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 1 cut(s) 392
BmiI GGNNCC 1 cut(s) 393
BmrFI CCNGG 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 49, 207, 226
BsaWI WCCGGW 1 cut(s) 424
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 3 cut(s) 49, 207, 226
BseMII CTCAG 1 cut(s) 401
Bsh1285I CGRYCG 2 cut(s) 187, 255
BsiEI CGRYCG 2 cut(s) 187, 255
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 405
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 1 cut(s) 564
Bsp13I TCCGGA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 8, 148, 252, 538, 588
Bsp19I CCATGG 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 400
BspEI TCCGGA 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 393
BssECI CCNNGG 3 cut(s) 49, 207, 226
BssMI GATC 5 cut(s) 8, 148, 252, 538, 588
BssNI GRCGYC 1 cut(s) 46
BssT1I CCWWGG 2 cut(s) 49, 207
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 3 cut(s) 342, 375, 500
BstACI GRCGYC 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 387
BstDSI CCRYGG 1 cut(s) 207
BstEII GGTNACC 1 cut(s) 570
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 5 cut(s) 11, 151, 255, 541, 591
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 5 cut(s) 8, 148, 252, 538, 588
BstMCI CGRYCG 2 cut(s) 187, 255
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 3 cut(s) 83, 119, 568
BstPI GGTNACC 1 cut(s) 570
BstSCI CCNGG 1 cut(s) 226
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 207
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 351, 505
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 1 cut(s) 392
CseI GACGC 1 cut(s) 54
CviAII CATG 6 cut(s) 80, 116, 133, 164, 208, 565
CviJI RGCY 5 cut(s) 105, 200, 206, 479, 544
CviKI_1 RGCY 5 cut(s) 105, 200, 206, 479, 544
DdeI CTNAG 1 cut(s) 387
DpnI GATC 5 cut(s) 10, 150, 254, 540, 590
DpnII GATC 5 cut(s) 8, 148, 252, 538, 588
DraIII CACNNNGTG 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 49, 207
Eco47I GGWCC 1 cut(s) 392
Eco88I CYCGRG 1 cut(s) 518
Eco91I GGTNACC 1 cut(s) 570
EcoO65I GGTNACC 1 cut(s) 570
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 49, 207
ErhI CCWWGG 2 cut(s) 49, 207
FaeI CATG 6 cut(s) 83, 119, 136, 167, 211, 568
FaqI GGGAC 1 cut(s) 405
FatI CATG 6 cut(s) 79, 115, 132, 163, 207, 564
FbaI TGATCA 2 cut(s) 148, 538
FblI GTMKAC 1 cut(s) 43
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 144
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 358
Hin1I GRCGYC 1 cut(s) 46
Hin1II CATG 6 cut(s) 83, 119, 136, 167, 211, 568
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 4 cut(s) 170, 193, 364, 406
HpaII CCGG 1 cut(s) 425
HphI GGTGA 2 cut(s) 503, 564
Hpy166II GTNNAC 2 cut(s) 44, 368
Hpy188I TCNGA 6 cut(s) 33, 192, 411, 467, 595, 642
Hpy188III TCNNGA 5 cut(s) 109, 146, 314, 425, 518
Hpy8I GTNNAC 2 cut(s) 44, 368
Hpy99I CGWCG 1 cut(s) 364
HpyCH4III ACNGT 3 cut(s) 342, 375, 500
HpyCH4IV ACGT 2 cut(s) 259, 321
HpyCH4V TGCA 2 cut(s) 167, 583
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 2 cut(s) 259, 321
Hsp92I GRCGYC 1 cut(s) 46
Hsp92II CATG 6 cut(s) 83, 119, 136, 167, 211, 568
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 424
Ksp22I TGATCA 2 cut(s) 148, 538
Kzo9I GATC 5 cut(s) 8, 148, 252, 538, 588
LpnPI CCDG 7 cut(s) 108, 159, 210, 213, 240, 374, 438
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 259, 321
MaeIII GTNAC 2 cut(s) 58, 570
MalI GATC 5 cut(s) 10, 150, 254, 540, 590
MboI GATC 5 cut(s) 8, 148, 252, 538, 588
MboII GAAGA 5 cut(s) 18, 292, 482, 485, 619
MflI RGATCY 1 cut(s) 8
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 5 cut(s) 19, 346, 511, 577, 658
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 529
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 2 cut(s) 261, 321
MseI TTAA 3 cut(s) 278, 524, 620
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
NcoI CCATGG 1 cut(s) 207
NdeII GATC 5 cut(s) 8, 148, 252, 538, 588
NlaIII CATG 6 cut(s) 83, 119, 136, 167, 211, 568
NlaIV GGNNCC 1 cut(s) 393
NmuCI GTSAC 2 cut(s) 58, 570
NspI RCATGY 3 cut(s) 83, 119, 568
PciI ACATGT 2 cut(s) 115, 564
PdmI GAANNNNTTC 2 cut(s) 261, 321
PfeI GAWTC 3 cut(s) 170, 193, 406
Ple19I CGATCG 1 cut(s) 255
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PscI ACATGT 2 cut(s) 115, 564
Psp1406I AACGTT 2 cut(s) 259, 321
Psp6I CCWGG 1 cut(s) 226
PspEI GGTNACC 1 cut(s) 570
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 1 cut(s) 392
PsuI RGATCY 1 cut(s) 8
PvuI CGATCG 1 cut(s) 255
SaqAI TTAA 3 cut(s) 278, 524, 620
Sau3AI GATC 5 cut(s) 8, 148, 252, 538, 588
Sau96I GGNCC 1 cut(s) 392
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 564
SetI ASST 6 cut(s) 202, 232, 262, 324, 546, 626
SinI GGWCC 1 cut(s) 392
Sse9I AATT 5 cut(s) 19, 346, 511, 577, 658
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 2 cut(s) 49, 207
TaaI ACNGT 3 cut(s) 342, 375, 500
TaiI ACGT 2 cut(s) 262, 324
TaqI TCGA 2 cut(s) 255, 362
TaqII GACCGA 3 cut(s) 173, 315, 620
TasI AATT 5 cut(s) 19, 346, 511, 577, 658
TfiI GAWTC 3 cut(s) 170, 193, 406
Tru1I TTAA 3 cut(s) 278, 524, 620
Tru9I TTAA 3 cut(s) 278, 524, 620
TscAI CASTG 2 cut(s) 358, 505
TseFI GTSAC 2 cut(s) 58, 570
Tsp45I GTSAC 2 cut(s) 58, 570
TspGWI ACGGA 2 cut(s) 29, 414
TspRI CASTG 2 cut(s) 358, 505
VpaK11BI GGWCC 1 cut(s) 392
XapI RAATTY 2 cut(s) 346, 577
XceI RCATGY 3 cut(s) 83, 119, 568
XmiI GTMKAC 1 cut(s) 43
XmnI GAANNNNTTC 2 cut(s) 261, 321
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.