MD03G1167500.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012390
Physical Location & Seq
Reverse (-)
22396919 .. 22397563
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1167500.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGGCAATGCAGTTTCTCCCTGCTTTCAGCAAAAAACAAACTCAGTTGTGAAAGTAATATTTTCAGAAGGCAGCACCACAAGATTCCTCAAAGGAAAACACATCGCTGGAGAGATCATGTTCGAGTTCCCTGACCAAATGGTTTGCCACGCAGACTCTTTCTTCATCGGCCGCCCGGTTCCTGCCTTAGCCATCAACGACGAGCTCATGCTTGGCCAGACCTATTTCGTTCTTCCGATTGATCGTTTCGCATGCAGCGTCCTCTCAGCCTCGACCCTCGCCGCCCTAGGCTCATCGTCGAGGCCCAACAAGAAGGCAGCTCCTATCAATTTCGGGGAGTGCCCGTTTGAGTATATAAAGGGTTCAAACGGAAAGGTCTTGATCAAGGTGGTGCCAGAGTTCATAGCAAGGCTAATTGCAAGAAGTCACAAGGAAATTGATGGGTCGTCTGAGGCTATAAGTTCTGTAAATACTACTTTTCTTTGTAGCACACCTGAGCTGCAAAAGCACTATGAACAACTCGTTGTGTCTAAAGAGCAATTATGGTCGCCTAAGCTTGAAACCATTTCTGAGTATAAGGTTAGGTTTTCACCATGCAAATATATAGGGCTGGAGTGGAAAGAAAAAGAGCAGGAAGTCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

215

Amino Acids

23.83

Weight (kDa)

7.59

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 191 1.3e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 391
AciI CCGC 2 cut(s) 172, 282
AcoI YGGCCR 2 cut(s) 169, 214
AgsI TTSAA 2 cut(s) 366, 560
AluBI AGCT 4 cut(s) 205, 320, 499, 556
AluI AGCT 4 cut(s) 205, 320, 499, 556
Alw21I GWGCWC 1 cut(s) 207
AoxI GGCC 3 cut(s) 169, 214, 302
ApeKI GCWGC 4 cut(s) 72, 255, 317, 499
AspA2I CCTAGG 1 cut(s) 286
AspS9I GGNCC 1 cut(s) 303
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 582
AvrII CCTAGG 1 cut(s) 286
BaeGI GKGCMC 1 cut(s) 344
BalI TGGCCA 1 cut(s) 216
BanI GGYRCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 207
BbvI GCAGC 4 cut(s) 84, 267, 329, 486
BccI CCATC 2 cut(s) 200, 434
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 176
BfaI CTAG 2 cut(s) 287, 643
BisI GCNGC 6 cut(s) 73, 172, 256, 282, 318, 500
BlnI CCTAGG 1 cut(s) 286
BlsI GCNGC 6 cut(s) 74, 173, 257, 283, 319, 501
Bme1390I CCNGG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 2 cut(s) 180, 393
BmrFI CCNGG 1 cut(s) 176
BpmI CTGGAG 2 cut(s) 129, 632
Bpu10I CCTNAGC 3 cut(s) 187, 495, 552
BpuMI CCSGG 1 cut(s) 176
BsaJI CCNNGG 1 cut(s) 286
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bse3DI GCAATG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 286
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 5 cut(s) 57, 279, 441, 486, 561
BseSI GKGCMC 1 cut(s) 344
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 4 cut(s) 84, 267, 329, 486
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 3 cut(s) 171, 216, 304
BshNI GGYRCC 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 176
BsnI GGCC 3 cut(s) 171, 216, 304
Bsp1286I GDGCHC 2 cut(s) 207, 344
Bsp143I GATC 3 cut(s) 114, 241, 381
BspACI CCGC 2 cut(s) 172, 282
BspANI GGCC 3 cut(s) 171, 216, 304
BspCNI CTCAG 5 cut(s) 56, 278, 442, 487, 562
BspLI GGNNCC 2 cut(s) 180, 393
BspT107I GGYRCC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 114, 241, 381
BssT1I CCWWGG 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 7 cut(s) 43, 187, 265, 450, 495, 552, 570
BstKTI GATC 3 cut(s) 117, 244, 384
BstMBI GATC 3 cut(s) 114, 241, 381
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 505
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 344
BstV1I GCAGC 4 cut(s) 84, 267, 329, 486
BstZI CGGCCG 1 cut(s) 169
BsuRI GGCC 3 cut(s) 171, 216, 304
BtgZI GCGATG 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 303
CseI GACGC 1 cut(s) 247
CviAII CATG 4 cut(s) 118, 208, 252, 594
DdeI CTNAG 7 cut(s) 43, 187, 265, 450, 495, 552, 570
DpnI GATC 3 cut(s) 116, 243, 383
DpnII GATC 3 cut(s) 114, 241, 381
EaeI YGGCCR 2 cut(s) 169, 214
EagI CGGCCG 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 207
Eco52I CGGCCG 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 4 cut(s) 121, 211, 255, 597
FatI CATG 4 cut(s) 117, 207, 251, 593
FbaI TGATCA 1 cut(s) 381
Fnu4HI GCNGC 6 cut(s) 73, 172, 256, 282, 318, 500
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 6 cut(s) 73, 172, 256, 282, 318, 500
FspBI CTAG 2 cut(s) 287, 643
GluI GCNGC 6 cut(s) 73, 172, 256, 282, 318, 500
GsuI CTGGAG 2 cut(s) 129, 632
HaeIII GGCC 3 cut(s) 171, 216, 304
HapII CCGG 1 cut(s) 176
HgaI GACGC 1 cut(s) 247
Hin1II CATG 4 cut(s) 121, 211, 255, 597
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 2 cut(s) 84, 155
HpaII CCGG 1 cut(s) 176
HphI GGTGA 1 cut(s) 582
Hpy188I TCNGA 4 cut(s) 67, 237, 451, 571
Hpy188III TCNNGA 1 cut(s) 379
Hpy99I CGWCG 2 cut(s) 203, 301
HpyAV CCTTC 2 cut(s) 62, 307
HpyCH4V TGCA 5 cut(s) 11, 255, 419, 502, 597
HpyF10VI GCNNNNNNNGC 1 cut(s) 505
HpyF3I CTNAG 7 cut(s) 43, 187, 265, 450, 495, 552, 570
Hsp92II CATG 4 cut(s) 121, 211, 255, 597
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 3 cut(s) 114, 241, 381
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 4 cut(s) 84, 267, 329, 486
MaeI CTAG 2 cut(s) 287, 643
MaeIII GTNAC 1 cut(s) 425
MalI GATC 3 cut(s) 116, 243, 383
MboI GATC 3 cut(s) 114, 241, 381
MboII GAAGA 2 cut(s) 154, 224
MhlI GDGCHC 2 cut(s) 207, 344
MlsI TGGCCA 1 cut(s) 216
MluCI AATT 4 cut(s) 328, 414, 435, 539
MluNI TGGCCA 1 cut(s) 216
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 6 cut(s) 98, 272, 280, 287, 294, 445
Mox20I TGGCCA 1 cut(s) 216
MscI TGGCCA 1 cut(s) 216
Msp20I TGGCCA 1 cut(s) 216
MspI CCGG 1 cut(s) 176
MspR9I CCNGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 505
NciI CCSGG 1 cut(s) 176
NdeII GATC 3 cut(s) 114, 241, 381
NlaIII CATG 4 cut(s) 121, 211, 255, 597
NlaIV GGNNCC 2 cut(s) 180, 393
NmuCI GTSAC 1 cut(s) 425
NspI RCATGY 1 cut(s) 255
PaeI GCATGC 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 6 cut(s) 74, 173, 257, 283, 319, 501
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 180, 393
PspPI GGNCC 1 cut(s) 303
SacI GAGCTC 1 cut(s) 207
SatI GCNGC 6 cut(s) 73, 172, 256, 282, 318, 500
Sau3AI GATC 3 cut(s) 114, 241, 381
Sau96I GGNCC 1 cut(s) 303
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 2 cut(s) 207, 344
SphI GCATGC 1 cut(s) 255
Sse9I AATT 4 cut(s) 328, 414, 435, 539
SsiI CCGC 2 cut(s) 172, 282
SspI AATATT 1 cut(s) 60
SspMI CTAG 2 cut(s) 287, 643
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 1 cut(s) 286
TaqI TCGA 3 cut(s) 123, 272, 299
TasI AATT 4 cut(s) 328, 414, 435, 539
TauI GCSGC 2 cut(s) 174, 284
TfiI GAWTC 1 cut(s) 84
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 4 cut(s) 72, 255, 317, 499
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 3 cut(s) 154, 391, 528
TspGWI ACGGA 1 cut(s) 384
XceI RCATGY 1 cut(s) 255
XmaJI CCTAGG 1 cut(s) 286
XspI CTAG 2 cut(s) 287, 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.