MD11G1186400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012390
Physical Location & Seq
Reverse (-)
25377767 .. 25378414
648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1186400.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGGCAATGCAGTTTCTCCCTGTTTCCAAAAAAAAGCTAACTCAGATGTGAAAGTAATATTTTCAGAAGGCAGCACAAGACTCCTCAAAGGAAAACACATCGCTGGAGAGATCATGTTTGAGTTTCCAGACCAAATGGTTTGCGACGCAGACTCTTTCTTCATCGGCCACCCCGTTCCAGCTTTAGCCATCGACGACGAGCTCATGCATGGCCAGACCTATTTCGTTCTTCCGATTGATCGTTTCGCATGTAGCGTCCTCACAGCCTCGACTCTTGCCGCCCTAGGCTCATCGTCAAGCCCTAACAAGAAGGCAGCTCCTATCAATTTTGGGGAGTGCCCGTTTGAGTACATAAAGAGTTCGAATGGAAAGGTCTTGATCAAGGTGGTACCGGAGTTCATAACAAGGTTAATTGCAAGAGGTCACAAGAAAATAGATGGGTCGTTTGATGATGCTGCTACTAGTTCTGGTAGTAGTACTTTTCTTTGTAGCACACCTGAGCTGCAAAAACACTATGAACAGCTTGTTGTGTCTAAAGAGCAATTATGGTCGCCTAAGCTTGAAACTATTTCTGAATACAAGGTTAGGTTTTCACCATGCAAATATATAGGGCTGGAGTGGAAACAAAAAGAGCAAGAACTCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

216

Amino Acids

23.79

Weight (kDa)

6.52

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 192 3.9e-40 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 388
AccB1I GGYRCC 1 cut(s) 388
AciI CCGC 1 cut(s) 279
AcoI YGGCCR 2 cut(s) 166, 211
AfaI GTAC 3 cut(s) 350, 390, 478
AgsI TTSAA 1 cut(s) 563
AhlI ACTAGT 1 cut(s) 461
AluBI AGCT 7 cut(s) 38, 182, 202, 317, 502, 523, 559
AluI AGCT 7 cut(s) 38, 182, 202, 317, 502, 523, 559
Alw21I GWGCWC 1 cut(s) 204
AoxI GGCC 2 cut(s) 166, 211
ApeKI GCWGC 4 cut(s) 72, 314, 455, 502
Asp718I GGTACC 1 cut(s) 388
AspA2I CCTAGG 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 585
AsuII TTCGAA 1 cut(s) 362
AvrII CCTAGG 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 341
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BalI TGGCCA 1 cut(s) 213
BanI GGYRCC 1 cut(s) 388
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 4 cut(s) 84, 326, 442, 489
BccI CCATC 2 cut(s) 197, 431
BclI TGATCA 1 cut(s) 378
BcuI ACTAGT 1 cut(s) 461
BfaI CTAG 2 cut(s) 284, 462
BisI GCNGC 5 cut(s) 73, 279, 315, 456, 503
BlnI CCTAGG 1 cut(s) 283
BlsI GCNGC 5 cut(s) 74, 280, 316, 457, 504
BmcAI AGTACT 1 cut(s) 478
BmiI GGNNCC 1 cut(s) 390
BmsI GCATC 1 cut(s) 442
BpmI CTGGAG 2 cut(s) 126, 635
Bpu10I CCTNAGC 2 cut(s) 498, 555
Bpu14I TTCGAA 1 cut(s) 362
BsaJI CCNNGG 1 cut(s) 283
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bse3DI GCAATG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 57, 489
BseRI GAGGAG 1 cut(s) 74
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 4 cut(s) 84, 326, 442, 489
BshFI GGCC 2 cut(s) 168, 213
BshNI GGYRCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 204
BsiSI CCGG 1 cut(s) 392
BsnI GGCC 2 cut(s) 168, 213
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 204, 341
Bsp143I GATC 3 cut(s) 111, 238, 378
BspACI CCGC 1 cut(s) 279
BspANI GGCC 2 cut(s) 168, 213
BspCNI CTCAG 2 cut(s) 56, 490
BspLI GGNNCC 1 cut(s) 390
BspT104I TTCGAA 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 3 cut(s) 111, 238, 378
BssT1I CCWWGG 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 362
BstDEI CTNAG 3 cut(s) 43, 498, 555
BstKTI GATC 3 cut(s) 114, 241, 381
BstMBI GATC 3 cut(s) 111, 238, 378
BstNSI RCATGY 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 341
BstV1I GCAGC 4 cut(s) 84, 326, 442, 489
BsuRI GGCC 2 cut(s) 168, 213
BtgZI GCGATG 1 cut(s) 85
CseI GACGC 2 cut(s) 155, 244
Csp6I GTAC 3 cut(s) 349, 389, 477
CviAII CATG 5 cut(s) 115, 205, 209, 249, 597
CviQI GTAC 3 cut(s) 349, 389, 477
DdeI CTNAG 3 cut(s) 43, 498, 555
DpnI GATC 3 cut(s) 113, 240, 380
DpnII GATC 3 cut(s) 111, 238, 378
EaeI YGGCCR 2 cut(s) 166, 211
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 202
EcoICRI GAGCTC 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 210
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 5 cut(s) 118, 208, 212, 252, 600
FatI CATG 5 cut(s) 114, 204, 208, 248, 596
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 5 cut(s) 73, 279, 315, 456, 503
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 5 cut(s) 73, 279, 315, 456, 503
FspBI CTAG 2 cut(s) 284, 462
GluI GCNGC 5 cut(s) 73, 279, 315, 456, 503
GsuI CTGGAG 2 cut(s) 126, 635
HaeIII GGCC 2 cut(s) 168, 213
HapII CCGG 1 cut(s) 392
HgaI GACGC 2 cut(s) 155, 244
Hin1II CATG 5 cut(s) 118, 208, 212, 252, 600
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 81, 152, 271
HpaII CCGG 1 cut(s) 392
HphI GGTGA 1 cut(s) 585
Hpy188I TCNGA 4 cut(s) 46, 67, 234, 574
Hpy188III TCNNGA 2 cut(s) 128, 376
Hpy99I CGWCG 3 cut(s) 149, 197, 200
HpyAV CCTTC 2 cut(s) 62, 304
HpyCH4V TGCA 5 cut(s) 11, 208, 416, 505, 600
HpyF3I CTNAG 3 cut(s) 43, 498, 555
Hsp92II CATG 5 cut(s) 118, 208, 212, 252, 600
KpnI GGTACC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 3 cut(s) 111, 238, 378
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 9 cut(s) 34, 90, 141, 192, 227, 405, 453, 510, 599
Lsp1109I GCAGC 4 cut(s) 84, 326, 442, 489
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 284, 462
MaeIII GTNAC 1 cut(s) 422
MalI GATC 3 cut(s) 113, 240, 380
MboI GATC 3 cut(s) 111, 238, 378
MboII GAAGA 2 cut(s) 151, 221
MhlI GDGCHC 2 cut(s) 204, 341
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 3 cut(s) 325, 411, 542
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 3 cut(s) 75, 146, 265
MnlI CCTC 4 cut(s) 95, 269, 277, 413
Mox20I TGGCCA 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 210
MscI TGGCCA 1 cut(s) 213
MseI TTAA 1 cut(s) 410
Msp20I TGGCCA 1 cut(s) 213
MspI CCGG 1 cut(s) 392
NdeII GATC 3 cut(s) 111, 238, 378
NlaIII CATG 5 cut(s) 118, 208, 212, 252, 600
NlaIV GGNNCC 1 cut(s) 390
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 210
NspI RCATGY 1 cut(s) 252
NspV TTCGAA 1 cut(s) 362
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 252
PkrI GCNGC 5 cut(s) 74, 280, 316, 457, 504
PleI GAGTC 3 cut(s) 75, 146, 265
PpsI GAGTC 3 cut(s) 75, 146, 265
Psp124BI GAGCTC 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 390
RsaI GTAC 3 cut(s) 350, 390, 478
RsaNI GTAC 3 cut(s) 349, 389, 477
SacI GAGCTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 5 cut(s) 73, 279, 315, 456, 503
Sau3AI GATC 3 cut(s) 111, 238, 378
ScaI AGTACT 1 cut(s) 478
SchI GAGTC 3 cut(s) 75, 146, 265
SduI GDGCHC 2 cut(s) 204, 341
SfaNI GCATC 1 cut(s) 442
SfuI TTCGAA 1 cut(s) 362
SpeI ACTAGT 1 cut(s) 461
Sse9I AATT 3 cut(s) 325, 411, 542
SsiI CCGC 1 cut(s) 279
SspI AATATT 1 cut(s) 60
SspMI CTAG 2 cut(s) 284, 462
SstI GAGCTC 1 cut(s) 204
StyI CCWWGG 1 cut(s) 283
TaqI TCGA 3 cut(s) 192, 269, 362
TasI AATT 3 cut(s) 325, 411, 542
TatI WGTACW 2 cut(s) 348, 476
TauI GCSGC 1 cut(s) 281
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 4 cut(s) 72, 314, 455, 502
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 3 cut(s) 151, 388, 531
XceI RCATGY 1 cut(s) 252
XmaJI CCTAGG 1 cut(s) 283
XspI CTAG 2 cut(s) 284, 462
ZrmI AGTACT 1 cut(s) 478
Zsp2I ATGCAT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.