Rorug05G0262800

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012390
Physical Location & Seq
Reverse (-)
29756350 .. 29758518
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0262800.1

Sequence Viewer

Length: 732 bp
ATGGGTTCAATACTTCAATTTCCTTTTGACTCAGAACATTATCACTACCGTCGCATCCGAGTCATTGGTAACTACCAAAAGAAGAGGTTTGCCCTCGTGACTTTGTCTAATACTACAGTACCGTTTTACCAAGGCCACAAGAACACCACCTTTTTGAGTGACATAATTGTTCAAGGGCAGCAGGTGGTGAAGTTTGGGGAAAAGGAGATTTCCGAGTTTAACAAGGAGACTGTTGCCGGAATTTACAGTATTGATGCAGTGCTCGCTCTTCGGGTGAAACTCAGGTTGGGCAAGATAAAGTTTCACAACTATGGCCCCGACAATATCCACTGCAAGCTGAAACTTCCTTTGAGTGCTAGAAATAGTACTTCTAATAGATTGGATTTCCAGCAGAAGGCTTTCGATCCTTGCTATTGGTCAACGATCTCTGTAGGCCCCAGTGAGTTAGAGACACCCACCCGTGCAGTCTCTCATGATTCTCGTCCTTTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTGGTGACATTATTGGCCTTGAATTAAAGGGAGATAATGCACTTATTGCTGCTGATATTGAGCGTTCTTCACTTGTTTTATGTGGCAATGCATTCTCAGATTCAAATGCAACTAAAGAAGCACTAGCTGTCTTGTCCGGACCTATTATATTTGCCCGAAGAGGCCTTCCCCTATCTGCACTAGAGTTTTATTTTGGATGCACTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

243

Amino Acids

26.95

Weight (kDa)

8.82

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 172
Acc36I ACCTGC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 524
AccIII TCCGGA 1 cut(s) 659
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 1 cut(s) 240
AfaI GTAC 2 cut(s) 120, 367
AfiI CCNNNNNNNGG 2 cut(s) 394, 524
AgsI TTSAA 5 cut(s) 9, 17, 173, 545, 627
AjuI GAANNNNNNNTTGG 2 cut(s) 269, 301
AluBI AGCT 2 cut(s) 337, 650
AluI AGCT 2 cut(s) 337, 650
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 3 cut(s) 221, 443, 472
AlwI GGATC 1 cut(s) 398
Aor13HI TCCGGA 1 cut(s) 659
AoxI GGCC 5 cut(s) 133, 313, 433, 538, 685
ApeKI GCWGC 2 cut(s) 178, 572
ApoI RAATTY 1 cut(s) 240
Asp700I GAANNNNTTC 1 cut(s) 398
AspS9I GGNCC 3 cut(s) 314, 434, 662
AsuHPI GGTGA 3 cut(s) 199, 286, 539
AvaII GGWCC 1 cut(s) 662
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BauI CACGAG 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 190, 559
BcoDI GTCTC 3 cut(s) 221, 443, 472
BfaI CTAG 3 cut(s) 357, 647, 704
BfmI CTRYAG 2 cut(s) 114, 429
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 2 cut(s) 179, 573
BlsI GCNGC 2 cut(s) 180, 574
BmcAI AGTACT 1 cut(s) 367
Bme18I GGWCC 1 cut(s) 662
BmgT120I GGNCC 3 cut(s) 314, 434, 662
BmiI GGNNCC 2 cut(s) 316, 436
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 3 cut(s) 63, 244, 710
BmuI ACTGGG 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 130
BsaWI WCCGGW 1 cut(s) 659
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 524
Bse1I ACTGG 1 cut(s) 438
Bse3DI GCAATG 1 cut(s) 616
BseAI TCCGGA 1 cut(s) 659
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 2 cut(s) 54, 725
BseLI CCNNNNNNNGG 2 cut(s) 394, 524
BseMI GCAATG 1 cut(s) 616
BseMII CTCAG 3 cut(s) 45, 295, 633
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 190, 559
BsgI GTGCAG 2 cut(s) 483, 684
BshFI GGCC 5 cut(s) 135, 315, 435, 540, 687
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 2 cut(s) 237, 660
BslI CCNNNNNNNGG 2 cut(s) 394, 524
BsmAI GTCTC 3 cut(s) 221, 443, 472
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 5 cut(s) 135, 315, 435, 540, 687
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 659
Bsp143I GATC 2 cut(s) 403, 423
BspANI GGCC 5 cut(s) 135, 315, 435, 540, 687
BspCNI CTCAG 3 cut(s) 44, 294, 632
BspEI TCCGGA 1 cut(s) 659
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 2 cut(s) 316, 436
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 1 cut(s) 398
BspQI GCTCTTC 1 cut(s) 273
BsrDI GCAATG 1 cut(s) 616
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 2 cut(s) 403, 423
BssSI CACGAG 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 95
Bst4CI ACNGT 6 cut(s) 50, 118, 123, 232, 248, 493
Bst6I CTCTTC 3 cut(s) 77, 273, 676
BstAPI GCANNNNNTGC 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 264, 335
BstDEI CTNAG 3 cut(s) 31, 281, 619
BstF5I GGATG 2 cut(s) 54, 725
BstKTI GATC 2 cut(s) 406, 426
BstMAI GTCTC 3 cut(s) 221, 443, 472
BstMBI GATC 2 cut(s) 403, 423
BstMWI GCNNNNNNNGC 2 cut(s) 263, 569
BstSFI CTRYAG 2 cut(s) 114, 429
BstV1I GCAGC 2 cut(s) 190, 559
BsuRI GGCC 5 cut(s) 135, 315, 435, 540, 687
BtsCI GGATG 2 cut(s) 54, 725
BtsI GCAGTG 3 cut(s) 264, 328, 723
BtsIMutI CAGTG 4 cut(s) 264, 328, 445, 723
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 2 cut(s) 264, 335
CciI TCATGA 1 cut(s) 472
Cfr13I GGNCC 3 cut(s) 314, 434, 662
Csp6I GTAC 2 cut(s) 119, 366
CviAII CATG 1 cut(s) 473
CviJI RGCY 9 cut(s) 135, 315, 337, 398, 435, 503, 540, 650, 687
CviKI_1 RGCY 9 cut(s) 135, 315, 337, 398, 435, 503, 540, 650, 687
CviQI GTAC 2 cut(s) 119, 366
DdeI CTNAG 3 cut(s) 31, 281, 619
DpnI GATC 2 cut(s) 405, 425
DpnII GATC 2 cut(s) 403, 423
Eam1104I CTCTTC 3 cut(s) 77, 273, 676
EarI CTCTTC 3 cut(s) 77, 273, 676
Eco130I CCWWGG 1 cut(s) 130
Eco147I AGGCCT 1 cut(s) 687
Eco47I GGWCC 1 cut(s) 662
EcoO109I RGGNCCY 1 cut(s) 434
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 476
FaiI YATR 7 cut(s) 164, 312, 474, 498, 512, 604, 671
FatI CATG 1 cut(s) 472
Fnu4HI GCNGC 2 cut(s) 179, 573
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 2 cut(s) 179, 573
FspBI CTAG 3 cut(s) 357, 647, 704
GluI GCNGC 2 cut(s) 179, 573
HaeIII GGCC 5 cut(s) 135, 315, 435, 540, 687
HapII CCGG 2 cut(s) 237, 660
Hin1II CATG 1 cut(s) 476
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HinfI GANTC 4 cut(s) 29, 60, 476, 623
HpaII CCGG 2 cut(s) 237, 660
HphI GGTGA 3 cut(s) 199, 286, 539
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 4 cut(s) 34, 59, 214, 622
Hpy188III TCNNGA 4 cut(s) 97, 473, 518, 660
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 2 cut(s) 388, 698
HpyCH4III ACNGT 6 cut(s) 50, 118, 123, 232, 248, 493
HpyCH4V TGCA 8 cut(s) 257, 333, 464, 563, 614, 632, 701, 723
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 569
HpyF3I CTNAG 3 cut(s) 31, 281, 619
Hsp92II CATG 1 cut(s) 476
Kpn2I TCCGGA 1 cut(s) 659
Kzo9I GATC 2 cut(s) 403, 423
LguI GCTCTTC 1 cut(s) 273
LpnPI CCDG 7 cut(s) 167, 250, 268, 401, 451, 531, 673
Lsp1109I GCAGC 2 cut(s) 190, 559
LweI GCATC 3 cut(s) 63, 244, 710
MaeI CTAG 3 cut(s) 357, 647, 704
MaeIII GTNAC 4 cut(s) 68, 97, 158, 527
MalI GATC 2 cut(s) 405, 425
MboI GATC 2 cut(s) 403, 423
MboII GAAGA 4 cut(s) 94, 260, 582, 693
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 4 cut(s) 17, 165, 240, 545
MlyI GAGTC 2 cut(s) 23, 69
MnlI CCTC 3 cut(s) 78, 104, 677
Mph1103I ATGCAT 1 cut(s) 616
MroI TCCGGA 1 cut(s) 659
MroXI GAANNNNTTC 1 cut(s) 398
MseI TTAA 3 cut(s) 219, 488, 548
MslI CAYNNNNRTG 1 cut(s) 309
MspI CCGG 2 cut(s) 237, 660
Mva1269I GAATGC 1 cut(s) 614
MwoI GCNNNNNNNGC 2 cut(s) 263, 569
NdeII GATC 2 cut(s) 403, 423
NlaIII CATG 1 cut(s) 476
NlaIV GGNNCC 2 cut(s) 316, 436
NmuCI GTSAC 3 cut(s) 97, 158, 527
NsiI ATGCAT 1 cut(s) 616
PagI TCATGA 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 172
PceI AGGCCT 1 cut(s) 687
PciSI GCTCTTC 1 cut(s) 273
PctI GAATGC 1 cut(s) 614
PdmI GAANNNNTTC 1 cut(s) 398
PfeI GAWTC 2 cut(s) 476, 623
PflFI GACNNNGTC 1 cut(s) 103
PflMI CCANNNNNTGG 1 cut(s) 524
PkrI GCNGC 2 cut(s) 180, 574
PleI GAGTC 2 cut(s) 23, 68
PpsI GAGTC 2 cut(s) 23, 68
PspN4I GGNNCC 2 cut(s) 316, 436
PspPI GGNCC 3 cut(s) 314, 434, 662
PsyI GACNNNGTC 1 cut(s) 103
RsaI GTAC 2 cut(s) 120, 367
RsaNI GTAC 2 cut(s) 119, 366
RseI CAYNNNNRTG 1 cut(s) 309
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 3 cut(s) 219, 488, 548
SatI GCNGC 2 cut(s) 179, 573
Sau3AI GATC 2 cut(s) 403, 423
Sau96I GGNCC 3 cut(s) 314, 434, 662
ScaI AGTACT 1 cut(s) 367
SchI GAGTC 2 cut(s) 23, 69
SduI GDGCHC 1 cut(s) 264
SetI ASST 7 cut(s) 89, 152, 186, 287, 339, 652, 667
SfaNI GCATC 3 cut(s) 63, 244, 710
SfcI CTRYAG 2 cut(s) 114, 429
SinI GGWCC 1 cut(s) 662
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 4 cut(s) 17, 165, 240, 545
SseBI AGGCCT 1 cut(s) 687
SspMI CTAG 3 cut(s) 357, 647, 704
StuI AGGCCT 1 cut(s) 687
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 6 cut(s) 50, 118, 123, 232, 248, 493
TaqI TCGA 1 cut(s) 402
TasI AATT 4 cut(s) 17, 165, 240, 545
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 2 cut(s) 476, 623
Tru1I TTAA 3 cut(s) 219, 488, 548
Tru9I TTAA 3 cut(s) 219, 488, 548
TscAI CASTG 4 cut(s) 264, 335, 445, 730
TseFI GTSAC 3 cut(s) 97, 158, 527
TseI GCWGC 2 cut(s) 178, 572
Tsp45I GTSAC 3 cut(s) 97, 158, 527
TspRI CASTG 4 cut(s) 264, 335, 445, 730
Tth111I GACNNNGTC 1 cut(s) 103
Van91I CCANNNNNTGG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 662
XapI RAATTY 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 398
XspI CTAG 3 cut(s) 357, 647, 704
ZrmI AGTACT 1 cut(s) 367
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.