FvH4_3g27940

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012390
Physical Location & Seq
Reverse (-)
20966054 .. 20967192
1139 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27940.t1

Sequence Viewer

Length: 645 bp
ATGGGTAATGCAGTGTCTCCATGTTTTCAACAAAACTCAAACTCGGCCGTCAAAGTAATCTTCTCCCAGCAAGGCACCACCAGGCTCCTCAAAGGAAAACACATCGCCGGAGAGATCATGTTCGAGTTTCCCGACCAAATGGTTTGCCATGCAGACTCTTTCTTCATCGGCCAACCTATTCCGGCCTTGTCCATCGACGACGAGCTCATGCTCGGCCAGACCTACTTCGTTCTTCCCCTCGACCGCTTCTCAGGCAGTGTGCTCTCCGCCTCCTCTCTCGCCGCGTTGAACTCATCGTCGGCGTCGAGCCCTAGGAGGAATTCTCCCATCAAATTTGGCGGGGAGAGCCCCTTTGAGTACATAAAGGGTTCCAATGGAAGGGTGCTGATCAAAGTTGTGCCGGAGTTCATCACAAGACTGATTTCAGCAAGGGATCAGGAAATAGGGTCCGAAAGCCCAAGCAACCGCTTTCTCTGTAACACACCGGAGCTTCAGAAACACTACGAGCAGCTTGTTGGCTCTAAAGAGCAATTATGGTCGCCTAAGCTTGAAACCATTTCAGAGCATAAGACCAGGTTGTCACCATGCAGATTTATAGGGCTCGAGTACTGGAAACCCAAAGAGATATTGCAAGTACTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

215

Amino Acids

23.83

Weight (kDa)

7.68

Isoelectric Point (pI)

52.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 188 2e-42 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 74
AccII CGCG 1 cut(s) 284
AciI CCGC 5 cut(s) 244, 267, 282, 339, 466
AclWI GGATC 1 cut(s) 441
AcoI YGGCCR 3 cut(s) 45, 169, 214
AcsI RAATTY 2 cut(s) 319, 332
AcuI CTGAAG 1 cut(s) 476
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 3 cut(s) 359, 608, 636
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 3 cut(s) 29, 289, 551
AjnI CCWGG 2 cut(s) 80, 572
AluBI AGCT 4 cut(s) 205, 490, 511, 547
AluI AGCT 4 cut(s) 205, 490, 511, 547
Alw21I GWGCWC 2 cut(s) 207, 264
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 4 cut(s) 45, 169, 183, 214
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 2 cut(s) 319, 332
AspA2I CCTAGG 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 573
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 1 cut(s) 447
AvrII CCTAGG 1 cut(s) 311
BanI GGYRCC 1 cut(s) 74
BanII GRGCYC 4 cut(s) 207, 311, 350, 603
Bbv12I GWGCWC 2 cut(s) 207, 264
BbvI GCAGC 1 cut(s) 520
BccI CCATC 2 cut(s) 200, 335
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 82, 574
BclI TGATCA 1 cut(s) 387
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 1 cut(s) 312
BisI GCNGC 2 cut(s) 282, 509
BlnI CCTAGG 1 cut(s) 311
BlsI GCNGC 2 cut(s) 283, 510
BmcAI AGTACT 2 cut(s) 608, 636
Bme1390I CCNGG 2 cut(s) 82, 574
Bme18I GGWCC 1 cut(s) 447
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 447
BmiI GGNNCC 4 cut(s) 76, 86, 370, 448
BmrFI CCNGG 2 cut(s) 82, 574
BplI GAGNNNNNCTC 2 cut(s) 307, 339
Bpu10I CCTNAGC 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 1 cut(s) 311
BsaWI WCCGGW 1 cut(s) 484
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 614
BseBI CCWGG 2 cut(s) 82, 574
BseDI CCNNGG 1 cut(s) 311
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 2 cut(s) 77, 262
BseX3I CGGCCG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 520
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 284
Bsh1285I CGRYCG 2 cut(s) 48, 244
BshFI GGCC 4 cut(s) 47, 171, 185, 216
BshNI GGYRCC 1 cut(s) 74
BsiEI CGRYCG 2 cut(s) 48, 244
BsiHKAI GWGCWC 2 cut(s) 207, 264
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 4 cut(s) 108, 182, 401, 485
BslI CCNNNNNNNGG 1 cut(s) 378
BsmAI GTCTC 1 cut(s) 21
BsnI GGCC 4 cut(s) 47, 171, 185, 216
BsoBI CYCGRG 1 cut(s) 602
Bsp1286I GDGCHC 5 cut(s) 207, 264, 311, 350, 603
Bsp143I GATC 3 cut(s) 114, 387, 433
BspACI CCGC 5 cut(s) 244, 267, 282, 339, 466
BspANI GGCC 4 cut(s) 47, 171, 185, 216
BspCNI CTCAG 1 cut(s) 263
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 4 cut(s) 76, 86, 370, 448
BspPI GGATC 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 74
BsrI ACTGG 1 cut(s) 614
BssECI CCNNGG 1 cut(s) 311
BssMI GATC 3 cut(s) 114, 387, 433
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 311
Bst2UI CCWGG 2 cut(s) 82, 574
BstACI GRCGYC 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 637
BstDEI CTNAG 2 cut(s) 250, 543
BstFNI CGCG 1 cut(s) 284
BstKTI GATC 3 cut(s) 117, 390, 436
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 3 cut(s) 114, 387, 433
BstMCI CGRYCG 2 cut(s) 48, 244
BstMWI GCNNNNNNNGC 3 cut(s) 252, 345, 637
BstNI CCWGG 2 cut(s) 82, 574
BstSCI CCNGG 2 cut(s) 80, 572
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 520
BstZI CGGCCG 1 cut(s) 45
BsuRI GGCC 4 cut(s) 47, 171, 185, 216
BtgZI GCGATG 1 cut(s) 88
BtsI GCAGTG 2 cut(s) 18, 262
BtsIMutI CAGTG 2 cut(s) 18, 262
Cfr13I GGNCC 1 cut(s) 447
CseI GACGC 1 cut(s) 291
CsiI ACCWGGT 1 cut(s) 572
Csp6I GTAC 3 cut(s) 358, 607, 635
CviAII CATG 5 cut(s) 21, 118, 149, 208, 585
CviQI GTAC 3 cut(s) 358, 607, 635
DdeI CTNAG 2 cut(s) 250, 543
DpnI GATC 3 cut(s) 116, 389, 435
DpnII GATC 3 cut(s) 114, 387, 433
DrdI GACNNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 3 cut(s) 45, 169, 214
EagI CGGCCG 1 cut(s) 45
EciI GGCGGA 1 cut(s) 256
Ecl136II GAGCTC 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 4 cut(s) 207, 311, 350, 603
Eco47I GGWCC 1 cut(s) 447
Eco52I CGGCCG 1 cut(s) 45
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 602
EcoICRI GAGCTC 1 cut(s) 205
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 2 cut(s) 80, 572
EcoT14I CCWWGG 1 cut(s) 311
EcoT38I GRGCYC 4 cut(s) 207, 311, 350, 603
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 5 cut(s) 24, 121, 152, 211, 588
FaiI YATR 9 cut(s) 22, 119, 150, 209, 362, 535, 567, 586, 596
FatI CATG 5 cut(s) 20, 117, 148, 207, 584
FauI CCCGC 1 cut(s) 332
FbaI TGATCA 1 cut(s) 387
Fnu4HI GCNGC 2 cut(s) 282, 509
FriOI GRGCYC 4 cut(s) 207, 311, 350, 603
Fsp4HI GCNGC 2 cut(s) 282, 509
FspBI CTAG 1 cut(s) 312
GluI GCNGC 2 cut(s) 282, 509
GsaI CCCAGC 1 cut(s) 70
HaeIII GGCC 4 cut(s) 47, 171, 185, 216
HapII CCGG 4 cut(s) 108, 182, 401, 485
HgaI GACGC 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 5 cut(s) 24, 121, 152, 211, 588
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 1 cut(s) 155
HpaII CCGG 4 cut(s) 108, 182, 401, 485
HphI GGTGA 1 cut(s) 573
Hpy188I TCNGA 3 cut(s) 451, 495, 562
Hpy188III TCNNGA 2 cut(s) 131, 437
Hpy99I CGWCG 4 cut(s) 200, 203, 301, 307
HpyAV CCTTC 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 11, 152, 588, 631
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 345, 637
HpyF3I CTNAG 2 cut(s) 250, 543
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 5 cut(s) 24, 121, 152, 211, 588
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 3 cut(s) 114, 387, 433
LmnI GCTCC 2 cut(s) 90, 487
Lsp1109I GCAGC 1 cut(s) 520
MabI ACCWGGT 1 cut(s) 572
MaeI CTAG 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 476, 579
MalI GATC 3 cut(s) 116, 389, 435
MboI GATC 3 cut(s) 114, 387, 433
MboII GAAGA 3 cut(s) 52, 154, 224
MhlI GDGCHC 5 cut(s) 207, 264, 311, 350, 603
MluCI AATT 3 cut(s) 319, 332, 530
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 5 cut(s) 98, 248, 280, 283, 309
MspI CCGG 4 cut(s) 108, 182, 401, 485
MspR9I CCNGG 2 cut(s) 82, 574
MvaI CCWGG 2 cut(s) 82, 574
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 3 cut(s) 252, 345, 637
NdeII GATC 3 cut(s) 114, 387, 433
NlaIII CATG 5 cut(s) 24, 121, 152, 211, 588
NlaIV GGNNCC 4 cut(s) 76, 86, 370, 448
NmeAIII GCCGAG 2 cut(s) 23, 192
NmuCI GTSAC 1 cut(s) 579
PaeR7I CTCGAG 1 cut(s) 602
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 302
PkrI GCNGC 2 cut(s) 283, 510
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 2 cut(s) 80, 572
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 2 cut(s) 80, 572
PspN4I GGNNCC 4 cut(s) 76, 86, 370, 448
PspPI GGNCC 1 cut(s) 447
PspXI VCTCGAGB 1 cut(s) 602
RsaI GTAC 3 cut(s) 359, 608, 636
RsaNI GTAC 3 cut(s) 358, 607, 635
SacI GAGCTC 1 cut(s) 207
SatI GCNGC 2 cut(s) 282, 509
Sau3AI GATC 3 cut(s) 114, 387, 433
Sau96I GGNCC 1 cut(s) 447
ScaI AGTACT 2 cut(s) 608, 636
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 82, 574
SduI GDGCHC 5 cut(s) 207, 264, 311, 350, 603
SetI ASST 7 cut(s) 178, 207, 224, 492, 513, 549, 578
SexAI ACCWGGT 1 cut(s) 572
Sfr274I CTCGAG 1 cut(s) 602
SinI GGWCC 1 cut(s) 447
SlaI CTCGAG 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 3 cut(s) 319, 332, 530
SsiI CCGC 5 cut(s) 244, 267, 282, 339, 466
SspMI CTAG 1 cut(s) 312
SstI GAGCTC 1 cut(s) 207
StyD4I CCNGG 2 cut(s) 80, 572
StyI CCWWGG 1 cut(s) 311
TaqI TCGA 5 cut(s) 123, 195, 240, 305, 603
TasI AATT 3 cut(s) 319, 332, 530
TatI WGTACW 3 cut(s) 357, 606, 634
TauI GCSGC 1 cut(s) 284
TscAI CASTG 2 cut(s) 18, 262
TseFI GTSAC 1 cut(s) 579
TseI GCWGC 1 cut(s) 508
Tsp45I GTSAC 1 cut(s) 579
TspDTI ATGAA 2 cut(s) 154, 397
TspRI CASTG 2 cut(s) 18, 262
VpaK11BI GGWCC 1 cut(s) 447
XapI RAATTY 2 cut(s) 319, 332
XhoI CTCGAG 1 cut(s) 602
XmaJI CCTAGG 1 cut(s) 311
XspI CTAG 1 cut(s) 312
ZrmI AGTACT 2 cut(s) 608, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.