Rroxscaffold_1G00030140

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012390
Physical Location & Seq
Forward (+)
39513155 .. 39516580
3426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00030140.1

Sequence Viewer

Length: 684 bp
ATGGGTAATGCAGTGTCTCCATGTTTTCAACAAAACTCAAACTCAGCTGTTAAAGTAATCTTTTCCCAAGGCACCACCAGACTCCTCAGAGGAAAACACATCGCCGGAGAGATCATGTTCGAGTTTCCCGACCAGATGGTTTGCCATGCAGACTCCTTCTTCATCGGTCAACCTATTCCGGCCTTGTCCATCGACGACGAGCTTATGCTCGGCCAGACCTACTTCGTTCTTCCCCTCGATCGCTTCGCAGGCAGTGTGCTCTCCGCCTCCTCTCTCGCGGCGTTAAACTCGTCGAGCCCTAAGAATTCTCCAATCAAATTCGGGGAGAGCCCCTTCGAGTACATAAAGGGTTCCAATGGAAAGGTGTTGATCAAAGTTGCGCCGGAGTTCATTACCAGGCTGATTACAAGAGGTCAGGAAATGGAGTCCGAAAGTCCAGGCAATCGTTTTCTCTGTAGCACACCGGAGCTTCAGAAACACTACGAGCAGCTTGTTGGTTCTAAAGAGCAATCGTGGTCGCCCAAGCTTGAAACCATTTCCGAGCATAAAATTAGATTTTCACCATGCAGATTCATAGGGCTGGAGTACTGGAAACCGAAAGAGACGCAAGTTTCTCAGTGGATACAGTCTGCTCTGTCTCCACTTTCACGCTCCCGTTACTTTCCTCTGTTATTGTCTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

227

Amino Acids

25.44

Weight (kDa)

8.66

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 181 1.6e-42 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccII CGCG 1 cut(s) 278
AciI CCGC 2 cut(s) 264, 278
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 304, 317
AcuI CTGAAG 1 cut(s) 455
AfaI GTAC 2 cut(s) 341, 587
AgsI TTSAA 2 cut(s) 29, 530
AjnI CCWGG 2 cut(s) 395, 436
AluBI AGCT 5 cut(s) 47, 202, 469, 490, 526
AluI AGCT 5 cut(s) 47, 202, 469, 490, 526
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 3 cut(s) 21, 596, 642
AoxI GGCC 2 cut(s) 180, 211
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 2 cut(s) 304, 317
AspLEI GCGC 1 cut(s) 382
AsuHPI GGTGA 1 cut(s) 552
BanI GGYRCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 299, 332
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 499
BccI CCATC 2 cut(s) 130, 197
BciT130I CCWGG 2 cut(s) 397, 438
BciVI GTATCC 1 cut(s) 615
BclI TGATCA 1 cut(s) 369
BcoDI GTCTC 3 cut(s) 21, 596, 642
BfmI CTRYAG 1 cut(s) 454
BfuI GTATCC 1 cut(s) 615
BisI GCNGC 2 cut(s) 279, 488
BlsI GCNGC 2 cut(s) 280, 489
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 2 cut(s) 397, 438
BmiI GGNNCC 2 cut(s) 73, 352
BmrFI CCNGG 2 cut(s) 397, 438
BpmI CTGGAG 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 463
Bse1I ACTGG 1 cut(s) 593
BseBI CCWGG 2 cut(s) 397, 438
BseDI CCNNGG 1 cut(s) 67
BseMII CTCAG 3 cut(s) 57, 100, 629
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 2 cut(s) 74, 259
BseXI GCAGC 1 cut(s) 499
Bsh1236I CGCG 1 cut(s) 278
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 2 cut(s) 182, 213
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 241
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 4 cut(s) 105, 179, 383, 464
BsmAI GTCTC 3 cut(s) 21, 596, 642
BsmBI CGTCTC 1 cut(s) 596
BsnI GGCC 2 cut(s) 182, 213
Bsp1286I GDGCHC 3 cut(s) 261, 299, 332
Bsp143I GATC 3 cut(s) 111, 238, 369
BspACI CCGC 2 cut(s) 264, 278
BspANI GGCC 2 cut(s) 182, 213
BspCNI CTCAG 3 cut(s) 56, 99, 628
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 73, 352
BspT107I GGYRCC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 3 cut(s) 111, 238, 369
BssT1I CCWWGG 1 cut(s) 67
Bst2UI CCWGG 2 cut(s) 397, 438
Bst4CI ACNGT 1 cut(s) 627
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 4 cut(s) 43, 86, 300, 615
BstFNI CGCG 1 cut(s) 278
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 3 cut(s) 114, 241, 372
BstMAI GTCTC 3 cut(s) 21, 596, 642
BstMBI GATC 3 cut(s) 111, 238, 369
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 249
BstNI CCWGG 2 cut(s) 397, 438
BstSCI CCNGG 2 cut(s) 395, 436
BstSFI CTRYAG 1 cut(s) 454
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 499
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 2 cut(s) 182, 213
BtgZI GCGATG 1 cut(s) 85
BtsI GCAGTG 2 cut(s) 18, 259
BtsIMutI CAGTG 3 cut(s) 18, 259, 623
Cac8I GCNNGC 1 cut(s) 250
CfoI GCGC 1 cut(s) 382
CseI GACGC 1 cut(s) 613
Csp6I GTAC 2 cut(s) 340, 586
CviAII CATG 4 cut(s) 21, 115, 146, 564
CviQI GTAC 2 cut(s) 340, 586
DdeI CTNAG 4 cut(s) 43, 86, 300, 615
DpnI GATC 3 cut(s) 113, 240, 371
DpnII GATC 3 cut(s) 111, 238, 369
EaeI YGGCCR 1 cut(s) 211
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 67
Eco24I GRGCYC 2 cut(s) 299, 332
Eco57I CTGAAG 1 cut(s) 455
EcoRI GAATTC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 395, 436
EcoT14I CCWWGG 1 cut(s) 67
EcoT38I GRGCYC 2 cut(s) 299, 332
ErhI CCWWGG 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 596
FaeI CATG 4 cut(s) 24, 118, 149, 567
FaiI YATR 8 cut(s) 22, 116, 147, 206, 344, 546, 565, 575
FatI CATG 4 cut(s) 20, 114, 145, 563
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 279, 488
FriOI GRGCYC 2 cut(s) 299, 332
Fsp4HI GCNGC 2 cut(s) 279, 488
GlaI GCGC 1 cut(s) 381
GluI GCNGC 2 cut(s) 279, 488
GsuI CTGGAG 1 cut(s) 602
HaeIII GGCC 2 cut(s) 182, 213
HapII CCGG 4 cut(s) 105, 179, 383, 464
HgaI GACGC 1 cut(s) 613
HhaI GCGC 1 cut(s) 382
Hin1II CATG 4 cut(s) 24, 118, 149, 567
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 1 cut(s) 170
HindII GTYRAC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 4 cut(s) 81, 152, 425, 570
HpaII CCGG 4 cut(s) 105, 179, 383, 464
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 1 cut(s) 170
Hpy188I TCNGA 4 cut(s) 89, 430, 474, 541
Hpy188III TCNNGA 2 cut(s) 128, 416
Hpy8I GTNNAC 1 cut(s) 170
Hpy99I CGWCG 3 cut(s) 197, 200, 295
HpyAV CCTTC 2 cut(s) 166, 343
HpyCH4III ACNGT 1 cut(s) 627
HpyCH4V TGCA 3 cut(s) 11, 149, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 249
HpyF3I CTNAG 4 cut(s) 43, 86, 300, 615
Hsp92II CATG 4 cut(s) 24, 118, 149, 567
HspAI GCGC 1 cut(s) 380
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 3 cut(s) 111, 238, 369
LmnI GCTCC 2 cut(s) 466, 656
Lsp1109I GCAGC 1 cut(s) 499
MaeIII GTNAC 1 cut(s) 656
MalI GATC 3 cut(s) 113, 240, 371
MboI GATC 3 cut(s) 111, 238, 369
MboII GAAGA 2 cut(s) 151, 221
MhlI GDGCHC 3 cut(s) 261, 299, 332
MluCI AATT 3 cut(s) 304, 317, 549
MlyI GAGTC 3 cut(s) 75, 146, 434
MnlI CCTC 7 cut(s) 83, 95, 245, 277, 280, 404, 675
MseI TTAA 2 cut(s) 51, 284
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 4 cut(s) 105, 179, 383, 464
MspR9I CCNGG 2 cut(s) 397, 438
MvaI CCWGG 2 cut(s) 397, 438
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 249
NdeII GATC 3 cut(s) 111, 238, 369
NlaIII CATG 4 cut(s) 24, 118, 149, 567
NlaIV GGNNCC 2 cut(s) 73, 352
NmeAIII GCCGAG 1 cut(s) 189
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 1 cut(s) 570
PkrI GCNGC 2 cut(s) 280, 489
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 3 cut(s) 75, 146, 433
PpsI GAGTC 3 cut(s) 75, 146, 433
Psp6I CCWGG 2 cut(s) 395, 436
PspGI CCWGG 2 cut(s) 395, 436
PspN4I GGNNCC 2 cut(s) 73, 352
PvuI CGATCG 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 2 cut(s) 341, 587
RsaNI GTAC 2 cut(s) 340, 586
SaqAI TTAA 2 cut(s) 51, 284
SatI GCNGC 2 cut(s) 279, 488
Sau3AI GATC 3 cut(s) 111, 238, 369
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 3 cut(s) 75, 146, 434
ScrFI CCNGG 2 cut(s) 397, 438
SduI GDGCHC 3 cut(s) 261, 299, 332
SetI ASST 9 cut(s) 49, 175, 204, 221, 366, 415, 471, 492, 528
SfcI CTRYAG 1 cut(s) 454
Sse9I AATT 3 cut(s) 304, 317, 549
SsiI CCGC 2 cut(s) 264, 278
StyD4I CCNGG 2 cut(s) 395, 436
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 627
TaqI TCGA 5 cut(s) 120, 192, 237, 293, 336
TaqII GACCGA 1 cut(s) 155
TasI AATT 3 cut(s) 304, 317, 549
TatI WGTACW 2 cut(s) 339, 585
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 1 cut(s) 570
Tru1I TTAA 2 cut(s) 51, 284
Tru9I TTAA 2 cut(s) 51, 284
TscAI CASTG 3 cut(s) 18, 259, 623
TseI GCWGC 1 cut(s) 487
TspDTI ATGAA 3 cut(s) 151, 379, 562
TspRI CASTG 3 cut(s) 18, 259, 623
XapI RAATTY 2 cut(s) 304, 317
ZrmI AGTACT 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.