RLG00000034716

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012390
Physical Location & Seq
Reverse (-)
52762942 .. 52763691
750 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034716

Sequence Viewer

Length: 411 bp
ATGACTTTCTCCAAGTCAAATCTATCCCCTCCCGGCTTCTTATCTTGTAGTGTGCTCTCCGCCTCCTCTCTTGCGGCGTTAAATTCGTCGAGCCCTAAGAATTCTCCGATCAAATTCGGGGAGAGCCCCTTCGAGTACATAAAGGGTTCCAATGGAAAGGTGTTGATCAAAGTTGCGCCGGAGTTCATTACCAGGCTGATTACAAGAGGTCAGGAAATGGAGTCCGAAAGTCCAGGCAATCGTTTTCTCTGTAGCACACCGGAGCTTCAGAAACACTACGAGCAGCTTGTTGGTTCTAAAGAGCAATTGTGGTCGCCCAAGCTTGAAACCATTTCTGAGCATAAAATTAGGTTTTCACCATGCAGATTCATAGGGCTGGAGTACTGGAAACCGAAAGAGACGCAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

137

Amino Acids

15.23

Weight (kDa)

9.03

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 17 - 113 4.4e-09 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12340 AT5G12340
fragaria_vesca FvH4_3g27940
malus_domestica MD03G1167500.v1.1 MD11G1186400.v1.1
prunus_persica Prupe.4G267600_v2.0.a1
pyrus_communis pycom03g12270 pycom11g16110
rosa_chinensis RchiOBHm_Chr5g0050661
rosa_laevigata RLG00000034716
rosa_multiflora Rmu_ssc0000350.1_g000037 Rmu_ssc0000350.1_g000038
rosa_roxburghii Rroxscaffold_1G00030140
rosa_rugosa Rorug05G0262800
rosa_samantha Rh5AG334800 Rh5BG344100 Rh5CG371900 Rh5DG357200
rosa_wichuraiana Rw5G031580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 60, 74
AcsI RAATTY 3 cut(s) 82, 100, 113
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 2 cut(s) 137, 383
AgsI TTSAA 1 cut(s) 326
AjnI CCWGG 2 cut(s) 191, 232
AluBI AGCT 3 cut(s) 265, 286, 322
AluI AGCT 3 cut(s) 265, 286, 322
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 3 cut(s) 82, 100, 113
AspLEI GCGC 1 cut(s) 178
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 1 cut(s) 348
BanII GRGCYC 2 cut(s) 95, 128
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 1 cut(s) 295
BciT130I CCWGG 2 cut(s) 193, 234
BclI TGATCA 1 cut(s) 165
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 392
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 2 cut(s) 75, 284
BlsI GCNGC 2 cut(s) 76, 285
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 3 cut(s) 33, 193, 234
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 3 cut(s) 33, 193, 234
BpmI CTGGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 259
Bse1I ACTGG 1 cut(s) 389
BseBI CCWGG 2 cut(s) 193, 234
BseMII CTCAG 1 cut(s) 327
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 3 cut(s) 33, 179, 260
BsmAI GTCTC 1 cut(s) 392
BsmBI CGTCTC 1 cut(s) 392
Bsp1286I GDGCHC 3 cut(s) 57, 95, 128
Bsp143I GATC 2 cut(s) 108, 165
BspACI CCGC 2 cut(s) 60, 74
BspCNI CTCAG 1 cut(s) 328
BspLI GGNNCC 1 cut(s) 148
BsrI ACTGG 1 cut(s) 389
BssMI GATC 2 cut(s) 108, 165
Bst2UI CCWGG 2 cut(s) 193, 234
BstDEI CTNAG 2 cut(s) 96, 336
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 2 cut(s) 111, 168
BstMAI GTCTC 1 cut(s) 392
BstMBI GATC 2 cut(s) 108, 165
BstNI CCWGG 2 cut(s) 193, 234
BstSCI CCNGG 3 cut(s) 31, 191, 232
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 295
CfoI GCGC 1 cut(s) 178
Csp6I GTAC 2 cut(s) 136, 382
CviAII CATG 1 cut(s) 360
CviJI RGCY 8 cut(s) 36, 93, 126, 196, 265, 286, 322, 376
CviKI_1 RGCY 8 cut(s) 36, 93, 126, 196, 265, 286, 322, 376
CviQI GTAC 2 cut(s) 136, 382
DdeI CTNAG 2 cut(s) 96, 336
DpnI GATC 2 cut(s) 110, 167
DpnII GATC 2 cut(s) 108, 165
EciI GGCGGA 1 cut(s) 49
Eco24I GRGCYC 2 cut(s) 95, 128
Eco57I CTGAAG 1 cut(s) 251
EcoRI GAATTC 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 191, 232
EcoT38I GRGCYC 2 cut(s) 95, 128
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 1 cut(s) 363
FaiI YATR 4 cut(s) 140, 342, 361, 371
FatI CATG 1 cut(s) 359
FbaI TGATCA 1 cut(s) 165
Fnu4HI GCNGC 2 cut(s) 75, 284
FriOI GRGCYC 2 cut(s) 95, 128
Fsp4HI GCNGC 2 cut(s) 75, 284
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 75, 284
GsuI CTGGAG 1 cut(s) 398
HapII CCGG 3 cut(s) 33, 179, 260
HhaI GCGC 1 cut(s) 178
Hin1II CATG 1 cut(s) 363
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 2 cut(s) 221, 366
HpaII CCGG 3 cut(s) 33, 179, 260
HphI GGTGA 1 cut(s) 348
Hpy188I TCNGA 4 cut(s) 108, 226, 270, 337
Hpy188III TCNNGA 1 cut(s) 212
Hpy99I CGWCG 1 cut(s) 91
HpyAV CCTTC 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 363
HpyF3I CTNAG 2 cut(s) 96, 336
Hsp92II CATG 1 cut(s) 363
HspAI GCGC 1 cut(s) 176
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 2 cut(s) 108, 165
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 1 cut(s) 295
MalI GATC 2 cut(s) 110, 167
MboI GATC 2 cut(s) 108, 165
MfeI CAATTG 1 cut(s) 305
MhlI GDGCHC 3 cut(s) 57, 95, 128
MluCI AATT 5 cut(s) 82, 100, 113, 305, 345
MlyI GAGTC 1 cut(s) 230
MnlI CCTC 4 cut(s) 39, 73, 76, 200
MseI TTAA 1 cut(s) 80
MspI CCGG 3 cut(s) 33, 179, 260
MspR9I CCNGG 3 cut(s) 33, 193, 234
MunI CAATTG 1 cut(s) 305
MvaI CCWGG 2 cut(s) 193, 234
NciI CCSGG 1 cut(s) 33
NdeII GATC 2 cut(s) 108, 165
NlaIII CATG 1 cut(s) 363
NlaIV GGNNCC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 366
PkrI GCNGC 2 cut(s) 76, 285
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 191, 232
PspGI CCWGG 2 cut(s) 191, 232
PspN4I GGNNCC 1 cut(s) 148
RsaI GTAC 2 cut(s) 137, 383
RsaNI GTAC 2 cut(s) 136, 382
SaqAI TTAA 1 cut(s) 80
SatI GCNGC 2 cut(s) 75, 284
Sau3AI GATC 2 cut(s) 108, 165
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 3 cut(s) 33, 193, 234
SduI GDGCHC 3 cut(s) 57, 95, 128
SetI ASST 6 cut(s) 162, 211, 267, 288, 324, 353
SfcI CTRYAG 1 cut(s) 250
Sse9I AATT 5 cut(s) 82, 100, 113, 305, 345
SsiI CCGC 2 cut(s) 60, 74
StyD4I CCNGG 3 cut(s) 31, 191, 232
TaqI TCGA 2 cut(s) 89, 132
TasI AATT 5 cut(s) 82, 100, 113, 305, 345
TatI WGTACW 2 cut(s) 135, 381
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 1 cut(s) 80
Tru9I TTAA 1 cut(s) 80
TseI GCWGC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 175, 358
XapI RAATTY 3 cut(s) 82, 100, 113
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.