AT5G12940

leucine-rich repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012849
Physical Location & Seq
Forward (+)
4087689 .. 4089146
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12940.1

Sequence Viewer

Length: 1116 bp
ATGGATACTAAGAAATCGTTAGTGATCCTATTGACTAACGTCGTCGTCTTCTTGCTTCTCTCCACGACTGTCCATTCTTGCTTACCGTCCGACCGAGCGGCGCTTCTAGAGTTCCGAGCAAAGCTCAACGAGCCCTACATTGGAGTTTTCAACACATGGAAAGGTCTAGACTGTTGCAAAGGCTGGTATGGTGTGAGCTGCGATCCCAACACCCGTCGAGTCGCCGGCATTACACTCCGAGGTGAATCCGAAGACCCGCTTTTCCAAAAGGCGAAACGGAGCGGACTAATGACCGGCTCCATTTCGCCCTCTATATGTAAGCTCACTCGTCTCTCCGGTATCATCATCGCTGATTGGAAGGGAATCTCCGGTGTGATTCCTAGTTGCATTGAGAATCTCCCTTTCTTGAGACATTTAGATCTCGTCGGAAATAAATTCTCCGGCGTGATTCCGGCGAATATCGGGAAGTTACTGAGGCTTAAAGTGTTGAATCTAGCTGATAATCACTTATACGGTGTGATTCCTCCGTCGATCACGAGATTAGTGAGTTTATCGCATTTGGATCTCAGGAATAACAATATCTCGGGAGTCATACCGCGAGATATTGGTCGTTTAAAGATGGTGAGTCGTGTCCTTTTAAGCGGTAACAAAATCTCGGGTCAAATACCCGATTCTCTGACCCGGATATACCGGCTCGCGGATCTTGAGCTCTCGATGAACCGACTCACCGGTCCGATTCCGGCTTCGTTCGGTAAAATGTCGGTGCTCGCGACTCTCAATCTCGATGGGAACTTGATCTCCGGTATGATTCCGGGGAGTCTATTGGCATCATCGATCTCGAATTTGAATCTGAGCGGGAATCTTATAACCGGAAGTATACCTAACACGTTTGGACCGAGATCGTACTTCACGGTGCTGGATCTTGCGAACAATCGTCTTCAGGGACCGATTCCGGCGAGTATCACGGCGGCGTCGTTTATTGGTCACCTTGACGTGAGCCATAACCATTTGTGTGGGAAGATTCCGATGGGATCTCCGTTTGATCATCTTGACGCGACGTCGTTTGCTTACAATGCTTGTCTTTGTGGAAAGCCTCTTGGGAATTGTAAGAAATAA

Protein Analysis

371

Amino Acids

39.95

Weight (kDa)

9.52

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 3.7e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 159 - 218 1e-08 Leucine rich repeat
LRR_8 PF13855 231 - 289 3.4e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012849)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20820 AT5G12940
fragaria_vesca FvH4_3g43270
malus_domestica MD03G1023300.v1.1 MD11G1028200.v1.1
prunus_persica Prupe.6G022400_v2.0.a1
pyrus_communis pycom03g01990 pycom11g02190
rosa_chinensis RchiOBHm_Chr5g0077331
rosa_laevigata RLG00000036686
rosa_multiflora Rmu_sc0000433.1_g000028
rosa_roxburghii Rroxscaffold_1G00004580
rosa_rugosa Rorug05G0453800
rosa_samantha Rh5AG509000 Rh5BG530300 Rh5CG554700 Rh5DG542600
rosa_wichuraiana Rw5G046840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
AasI GACNNNNNNGTC 1 cut(s) 729
AatII GACGTC 1 cut(s) 1061
AccBSI CCGCTC 3 cut(s) 98, 282, 855
AccI GTMKAC 1 cut(s) 877
AccII CGCG 4 cut(s) 598, 698, 770, 1055
AciI CCGC 8 cut(s) 98, 257, 282, 596, 642, 698, 855, 968
AclWI GGATC 6 cut(s) 19, 197, 570, 708, 927, 1039
AcsI RAATTY 2 cut(s) 434, 841
AcuI CTGAAG 1 cut(s) 923
AcyI GRCGYC 2 cut(s) 971, 1058
AfaI GTAC 1 cut(s) 905
AfiI CCNNNNNNNGG 2 cut(s) 140, 697
AflIII ACRYGT 1 cut(s) 885
AgeI ACCGGT 1 cut(s) 728
AgsI TTSAA 3 cut(s) 151, 490, 847
AhdI GACNNNNNGTC 1 cut(s) 1057
AjiI CACGTC 1 cut(s) 994
AloI GAACNNNNNNTCC 2 cut(s) 782, 814
AluBI AGCT 5 cut(s) 124, 198, 322, 497, 709
AluI AGCT 5 cut(s) 124, 198, 322, 497, 709
Alw21I GWGCWC 2 cut(s) 711, 768
Alw26I GTCTC 2 cut(s) 335, 403
AlwI GGATC 6 cut(s) 19, 197, 570, 708, 927, 1039
Ama87I CYCGRG 2 cut(s) 583, 655
ApeKI GCWGC 1 cut(s) 198
ApoI RAATTY 2 cut(s) 434, 841
AsiGI ACCGGT 1 cut(s) 728
AspLEI GCGC 1 cut(s) 103
AspS9I GGNCC 3 cut(s) 731, 893, 944
AsuC2I CCSGG 2 cut(s) 682, 813
AsuHPI GGTGA 4 cut(s) 254, 634, 718, 977
AvaI CYCGRG 2 cut(s) 583, 655
AvaII GGWCC 3 cut(s) 731, 893, 944
BanII GRGCYC 2 cut(s) 135, 711
BauI CACGAG 1 cut(s) 535
BbsI GAAGAC 3 cut(s) 40, 258, 929
Bbv12I GWGCWC 2 cut(s) 711, 768
BbvI GCAGC 1 cut(s) 185
BccI CCATC 3 cut(s) 613, 779, 1021
BceAI ACGGC 1 cut(s) 981
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 2 cut(s) 682, 813
BcoDI GTCTC 2 cut(s) 335, 403
BfaI CTAG 4 cut(s) 107, 167, 381, 494
BfoI RGCGCY 1 cut(s) 104
BglII AGATCT 1 cut(s) 418
BisI GCNGC 3 cut(s) 99, 199, 969
BlsI GCNGC 3 cut(s) 100, 200, 970
Bme1390I CCNGG 2 cut(s) 682, 813
Bme18I GGWCC 3 cut(s) 731, 893, 944
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmeT110I CYCGRG 2 cut(s) 583, 655
BmgBI CACGTC 1 cut(s) 994
BmgT120I GGNCC 3 cut(s) 731, 893, 944
BmiI GGNNCC 2 cut(s) 298, 945
BmrFI CCNGG 2 cut(s) 682, 813
BmsI GCATC 1 cut(s) 836
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 3 cut(s) 40, 258, 929
BplI GAGNNNNNCTC 2 cut(s) 108, 140
BpuEI CTTGAG 2 cut(s) 427, 725
BpuMI CCSGG 2 cut(s) 682, 813
Bsa29I ATCGAT 1 cut(s) 833
BsaHI GRCGYC 2 cut(s) 971, 1058
BsaJI CCNNGG 2 cut(s) 238, 812
BsaWI WCCGGW 5 cut(s) 335, 368, 728, 800, 869
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 697
Bse118I RCCGGY 4 cut(s) 224, 293, 690, 728
BseCI ATCGAT 1 cut(s) 833
BseDI CCNNGG 2 cut(s) 238, 812
BseLI CCNNNNNNNGG 2 cut(s) 140, 697
BseMII CTCAG 3 cut(s) 464, 580, 842
BseXI GCAGC 1 cut(s) 185
Bsh1236I CGCG 4 cut(s) 598, 698, 770, 1055
Bsh1285I CGRYCG 1 cut(s) 94
BshTI ACCGGT 1 cut(s) 728
BshVI ATCGAT 1 cut(s) 833
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 711, 768
BsiHKCI CYCGRG 2 cut(s) 583, 655
BslFI GGGAC 1 cut(s) 957
BslI CCNNNNNNNGG 2 cut(s) 140, 697
BsmAI GTCTC 2 cut(s) 335, 403
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 957
BsoBI CYCGRG 2 cut(s) 583, 655
Bsp1286I GDGCHC 3 cut(s) 135, 711, 768
Bsp68I TCGCGA 1 cut(s) 770
BspACI CCGC 8 cut(s) 98, 257, 282, 596, 642, 698, 855, 968
BspCNI CTCAG 3 cut(s) 465, 579, 843
BspDI ATCGAT 1 cut(s) 833
BspFNI CGCG 4 cut(s) 598, 698, 770, 1055
BspLI GGNNCC 2 cut(s) 298, 945
BspPI GGATC 6 cut(s) 19, 197, 570, 708, 927, 1039
BsrBI CCGCTC 3 cut(s) 98, 282, 855
BsrFI RCCGGY 4 cut(s) 224, 293, 690, 728
BssAI RCCGGY 4 cut(s) 224, 293, 690, 728
BssECI CCNNGG 2 cut(s) 238, 812
BssNAI GTATAC 1 cut(s) 878
BssNI GRCGYC 2 cut(s) 971, 1058
BssSI CACGAG 1 cut(s) 535
Bst1107I GTATAC 1 cut(s) 878
Bst2BI CACGAG 1 cut(s) 535
Bst4CI ACNGT 5 cut(s) 70, 87, 173, 515, 913
BstACI GRCGYC 2 cut(s) 971, 1058
BstC8I GCNNGC 3 cut(s) 226, 696, 768
BstDEI CTNAG 4 cut(s) 9, 473, 566, 851
BstEII GGTNACC 1 cut(s) 983
BstFNI CGCG 4 cut(s) 598, 698, 770, 1055
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 1 cut(s) 103
BstMAI GTCTC 2 cut(s) 335, 403
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 2 cut(s) 130, 1073
BstPAI GACNNNNGTC 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 983
BstSCI CCNGG 2 cut(s) 680, 811
BstUI CGCG 4 cut(s) 598, 698, 770, 1055
BstV1I GCAGC 1 cut(s) 185
BstV2I GAAGAC 3 cut(s) 40, 258, 929
BstX2I RGATCY 5 cut(s) 418, 562, 700, 919, 1031
BstXI CCANNNNNNTGG 1 cut(s) 1013
BstYI RGATCY 5 cut(s) 418, 562, 700, 919, 1031
BstZ17I GTATAC 1 cut(s) 878
Bsu15I ATCGAT 1 cut(s) 833
BsuTUI ATCGAT 1 cut(s) 833
BtgZI GCGATG 1 cut(s) 331
BtrI CACGTC 1 cut(s) 994
BtuMI TCGCGA 1 cut(s) 770
Cac8I GCNNGC 3 cut(s) 226, 696, 768
CfoI GCGC 1 cut(s) 103
Cfr10I RCCGGY 4 cut(s) 224, 293, 690, 728
Cfr13I GGNCC 3 cut(s) 731, 893, 944
ClaI ATCGAT 1 cut(s) 833
CpoI CGGWCCG 1 cut(s) 731
CseI GACGC 2 cut(s) 960, 1061
Csp6I GTAC 1 cut(s) 904
CspAI ACCGGT 1 cut(s) 728
CspI CGGWCCG 1 cut(s) 731
CviAII CATG 1 cut(s) 156
CviQI GTAC 1 cut(s) 904
DdeI CTNAG 4 cut(s) 9, 473, 566, 851
DraI TTTAAA 1 cut(s) 615
DrdI GACNNNNNNGTC 1 cut(s) 729
DriI GACNNNNNGTC 1 cut(s) 1057
DseDI GACNNNNNNGTC 1 cut(s) 729
Eam1105I GACNNNNNGTC 1 cut(s) 1057
Ecl136II GAGCTC 1 cut(s) 709
Eco24I GRGCYC 2 cut(s) 135, 711
Eco47I GGWCC 3 cut(s) 731, 893, 944
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 1 cut(s) 923
Eco88I CYCGRG 2 cut(s) 583, 655
Eco91I GGTNACC 1 cut(s) 983
EcoICRI GAGCTC 1 cut(s) 709
EcoO65I GGTNACC 1 cut(s) 983
EcoT38I GRGCYC 2 cut(s) 135, 711
Esp3I CGTCTC 1 cut(s) 335
FaeI CATG 1 cut(s) 159
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 1 cut(s) 957
FatI CATG 1 cut(s) 155
FauI CCCGC 2 cut(s) 264, 848
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 877
Fnu4HI GCNGC 3 cut(s) 99, 199, 969
FriOI GRGCYC 2 cut(s) 135, 711
Fsp4HI GCNGC 3 cut(s) 99, 199, 969
FspBI CTAG 4 cut(s) 107, 167, 381, 494
GlaI GCGC 1 cut(s) 102
GluI GCNGC 3 cut(s) 99, 199, 969
HaeII RGCGCY 1 cut(s) 104
HgaI GACGC 2 cut(s) 960, 1061
HhaI GCGC 1 cut(s) 103
Hin1I GRCGYC 2 cut(s) 971, 1058
Hin1II CATG 1 cut(s) 159
Hin6I GCGC 1 cut(s) 101
HinP1I GCGC 1 cut(s) 101
HphI GGTGA 4 cut(s) 254, 634, 718, 977
Hpy166II GTNNAC 1 cut(s) 878
Hpy188I TCNGA 9 cut(s) 91, 116, 239, 250, 428, 678, 735, 852, 1026
Hpy8I GTNNAC 1 cut(s) 878
Hpy99I CGWCG 8 cut(s) 44, 47, 219, 428, 532, 976, 1060, 1063
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 5 cut(s) 70, 87, 173, 515, 913
HpyCH4IV ACGT 4 cut(s) 39, 887, 993, 1058
HpyCH4V TGCA 2 cut(s) 177, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 1073
HpyF3I CTNAG 4 cut(s) 9, 473, 566, 851
HpySE526I ACGT 4 cut(s) 39, 887, 993, 1058
Hsp92I GRCGYC 2 cut(s) 971, 1058
Hsp92II CATG 1 cut(s) 159
HspAI GCGC 1 cut(s) 101
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 1042
LmnI GCTCC 2 cut(s) 279, 302
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 1 cut(s) 836
MaeI CTAG 4 cut(s) 107, 167, 381, 494
MaeII ACGT 4 cut(s) 39, 887, 993, 1058
MaeIII GTNAC 3 cut(s) 468, 644, 983
MbiI CCGCTC 3 cut(s) 98, 282, 855
MboII GAAGA 4 cut(s) 40, 263, 929, 1030
MflI RGATCY 5 cut(s) 418, 562, 700, 919, 1031
MhlI GDGCHC 3 cut(s) 135, 711, 768
MluCI AATT 3 cut(s) 434, 841, 1102
MlyI GAGTC 6 cut(s) 228, 597, 634, 717, 766, 826
MmeI TCCRAC 2 cut(s) 114, 406
MnlI CCTC 5 cut(s) 233, 319, 468, 534, 1104
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 3 cut(s) 480, 614, 638
MslI CAYNNNNRTG 1 cut(s) 1011
MspR9I CCNGG 2 cut(s) 682, 813
MvnI CGCG 4 cut(s) 598, 698, 770, 1055
MwoI GCNNNNNNNGC 2 cut(s) 130, 1073
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 2 cut(s) 682, 813
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 1 cut(s) 159
NlaIV GGNNCC 2 cut(s) 298, 945
NmuCI GTSAC 1 cut(s) 983
NruI TCGCGA 1 cut(s) 770
PcsI WCGNNNNNNNCGW 3 cut(s) 767, 893, 971
PdiI GCCGGC 1 cut(s) 226
PinAI ACCGGT 1 cut(s) 728
PkrI GCNGC 3 cut(s) 100, 200, 970
PleI GAGTC 6 cut(s) 227, 596, 633, 717, 766, 825
PpsI GAGTC 6 cut(s) 227, 596, 633, 717, 766, 825
PshAI GACNNNNGTC 1 cut(s) 38
PsiI TTATAA 1 cut(s) 866
Psp124BI GAGCTC 1 cut(s) 711
PspEI GGTNACC 1 cut(s) 983
PspN4I GGNNCC 2 cut(s) 298, 945
PspPI GGNCC 3 cut(s) 731, 893, 944
PsuI RGATCY 5 cut(s) 418, 562, 700, 919, 1031
RruI TCGCGA 1 cut(s) 770
RsaI GTAC 1 cut(s) 905
RsaNI GTAC 1 cut(s) 904
RseI CAYNNNNRTG 1 cut(s) 1011
Rsr2I CGGWCCG 1 cut(s) 731
RsrII CGGWCCG 1 cut(s) 731
SacI GAGCTC 1 cut(s) 711
SaqAI TTAA 3 cut(s) 480, 614, 638
SatI GCNGC 3 cut(s) 99, 199, 969
Sau96I GGNCC 3 cut(s) 731, 893, 944
SchI GAGTC 6 cut(s) 228, 597, 634, 717, 766, 826
ScrFI CCNGG 2 cut(s) 682, 813
SduI GDGCHC 3 cut(s) 135, 711, 768
SfaNI GCATC 1 cut(s) 836
SinI GGWCC 3 cut(s) 731, 893, 944
SmiMI CAYNNNNRTG 1 cut(s) 1011
SmlI CTYRAG 2 cut(s) 406, 704
SmoI CTYRAG 2 cut(s) 406, 704
Sse9I AATT 3 cut(s) 434, 841, 1102
SsiI CCGC 8 cut(s) 98, 257, 282, 596, 642, 698, 855, 968
SspMI CTAG 4 cut(s) 107, 167, 381, 494
SstI GAGCTC 1 cut(s) 711
StyD4I CCNGG 2 cut(s) 680, 811
TaaI ACNGT 5 cut(s) 70, 87, 173, 515, 913
TaiI ACGT 4 cut(s) 42, 890, 996, 1061
TaqI TCGA 6 cut(s) 217, 530, 713, 783, 833, 839
TaqII GACCGA 3 cut(s) 108, 910, 961
TasI AATT 3 cut(s) 434, 841, 1102
TauI GCSGC 2 cut(s) 101, 971
Tru1I TTAA 3 cut(s) 480, 614, 638
Tru9I TTAA 3 cut(s) 480, 614, 638
TseFI GTSAC 1 cut(s) 983
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 983
TspDTI ATGAA 1 cut(s) 731
TspGWI ACGGA 3 cut(s) 292, 516, 1026
VpaK11BI GGWCC 3 cut(s) 731, 893, 944
XapI RAATTY 2 cut(s) 434, 841
XbaI TCTAGA 2 cut(s) 106, 166
XmiI GTMKAC 1 cut(s) 877
XspI CTAG 4 cut(s) 107, 167, 381, 494
ZraI GACGTC 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.